A set of 3D cell culture colloids and a method for culturing those 3D cells.

A 3D cell culture method using sodium alginate and gelatin-based colloids with controlled formation and dissolution steps addresses the impracticalities of Matrigel, enabling efficient and wide-ranging 3D cell culture with faster spheroid formation and easy analysis.

JP7884846B2Inactive Publication Date: 2026-07-06GECOLL BIOMEDICAL CO LTD
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Patent Information

Authority / Receiving Office
JP · JP
Patent Type
Patents
Current Assignee / Owner
GECOLL BIOMEDICAL CO LTD
Filing Date
2020-08-26
Publication Date
2026-07-06
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

Conventional 3D cell culture methods using Matrigel face challenges such as complex preparation, difficulty in controlling colloid formation and concentration, high cost, and susceptibility to coagulation at room temperature, making them impractical for widespread use.

Method used

A 3D cell culture method using a set of colloids comprising gel material A, buffer C, and buffer D, involving steps like forming a colloid at low temperature, crosslinking with buffer C, and dissolving with buffer D for easy cell analysis, utilizing components like sodium alginate, gelatin, and divalent cation salts.

Benefits of technology

The method enables convenient and efficient 3D cell culture of various cell lines with controlled colloid formation, faster spheroid formation, and easy post-culture analysis, overcoming the limitations of Matrigel-based methods.

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Abstract

A set of 3D cell culture colloids and a method for 3D cell culture therewith are provided. [Solution] The 3D cell culture colloid set includes gel material A, buffer solution C, and buffer solution D. The 3D cell culture method includes the steps of adding cells to a mixture containing gel material A, allowing it to react at low temperature to obtain a colloid containing the cells, adding buffer solution C to the colloid to crosslink it, removing buffer solution C and adding a growth medium, and culturing the cells until spheroids are formed within the colloid. The present invention also further dissolves the colloid with buffer solution D, and extracts and analyzes the cells after culturing. Therefore, the 3D cell culture colloid set of the present invention is easy to use and can be used for 3D cell culture of a variety of cell lines.
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Citation Information

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