Cosmetic composition comprising a combination of at least one oligosaccharide and / or polysaccharide combined with mannose monosaccharide, and its use in maintaining the balance of skin bacterial flora.

Abstract

The present invention relates to a cosmetic composition for topical application, comprising, in a physiologically acceptable medium, at least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soybean-derived oligosaccharides, pyrodextrin, isomaltooligosaccharides, xylooligosaccharides, transgalactooligosaccharides and mixtures thereof, and at least one mannose monosaccharide. The present invention also relates to a cosmetic treatment method for the care of the skin and / or mucous membranes, comprising the application of said composition, in particular to skin and / or mucous membranes subjected to external aggression, and to its cosmetic use.

Description

[Technical Field]

[0001] The present invention relates to a cosmetic composition for topical application, comprising a combination of at least one oligosaccharide and / or polysaccharide combined with a mannose monosaccharide in a physiologically acceptable medium. The present invention also relates to the use and methods of cosmetic treatments that utilize this combination in maintaining and / or restoring the balance of the bacterial flora of the skin and / or mucous membranes. [Background technology]

[0002] Human skin is composed of two compartments: the dermis, which is the deep compartment, and the epidermis, which is the surface compartment.

[0003] The skin plays a major role in protecting against external attacks, such as environmental attacks, climatic influences (heat, cold, UV rays, tobacco, etc.), pollution, allergens, pathogenic bacteria, mechanical attacks (hair removal, shaving, abrasive washing), and chemical attacks (detergents). This property, known as the barrier function, is primarily performed by the outermost layer of the epidermis, namely the stratum corneum or keratin layer.

[0004] The skin also represents a complex ecosystem where several types of microorganisms, such as bacteria and fungi, proliferate. These microorganisms constitute the skin flora, also known as the microbial skin bacterial flora.

[0005] To date, over 500 bacterial species have been detected on healthy skin, containing more than 2 million genes. (Skin 1cm²) 2 Approximately 10 6 The bacteria present. The skin microbiome of healthy individuals is described as exhibiting specificity in three areas: wet, dry, and oily.

[0006] In particular, the following can be identified: - The beneficial symbiotic resident bacterial flora consists of microorganisms that obtain their nutrients from the skin, provide known benefits to the skin, and proliferate conventionally and permanently on healthy skin; a particular representative is the strain of Staphylococcus epidermidis. This strain is particularly found on the surface of healthy skin and is involved in maintaining the balance of the symbiotic skin flora. - For example, transient flora present on skin under abnormal conditions due to contact with contaminants; if it proliferates, this flora can become pathogenic. A possible example is Staphylococcus aureus, which is potentially pathogenic to human skin.

[0007] For example, if the barrier is adversely affected after an external attack, or if the balance between symbiotic organisms and pathogens is disrupted, then, for example, inflammation, stinging, pulling, redness, itching, or even skin diseases, such as deterioration of skin characteristics and / or skin infections, may occur (Non-Patent Literature 1; Non-Patent Literature 2).

[0008] Furthermore, it is clear that the characteristics of the skin barrier and mucous membranes are affected daily by external attacks from irritants (detergents, surfactants, acids, bases, oxidizing agents, reducing agents, concentrated solvents), mechanical stress (friction, scrubbing, shaving, or hair removal), or heat or climatic imbalances (cold, dryness).

[0009] Therefore, in particular the following [1]~[9] Due to their effects, the (re)colonization of the skin by beneficial microorganisms of the symbiotic skin flora appears to be fundamental to skin physiology and immunity: - Limitation of the adhesion of pathogenic microorganisms (by stimulating antimicrobial peptides or competitive adhesion); - Inflammation and immune dysregulation; - scar formation; - In particular, the restoration / recovery of the barrier function following the external attacks mentioned above.

[0010] Cosmetic solutions that are known to act on the microbial skin flora already exist.

[0011] (Patent Document 1) provides a composition of prebiotic lipids in particular, and probiotics for correcting dysbiosis (colony formation of pathogenic microorganisms) or maintaining the balance of beneficial symbiotic flora, such as Bacillus licheniformis, Bifidobacterium breve, Bifidobacterium infantis, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus sakei, Lactobacillus paracasei, and Staphylococcus epidermidis. The invention describes a product comprising a composition containing epidermidis and Staphylococcus xylosus.

[0012] Furthermore, (Patent Document 2) discloses the use of pullulan or its derivatives or hyaluronic acid or its salts or derivatives, alginic acid, its salts or derivatives, and a combination thereof of polysaccharides selected from mixtures of these polysaccharides for maintaining and / or preserving the balance of the microbial skin and / or mucosal flora.

[0013] (Patent Document 3) discloses a prebiotic skincare mask containing alpha-gluco-oligosaccharides, inulin, yeast, and bacteria of the genus Lactobacillus, particularly for enhancing the presence of healthy skin flora and preventing the formation of skin colonies by pathogens.

[0014] Nevertheless, among consumers, there is an ever - increasing need for careful treatment of the skin, particularly the skin flora, which has led to the search for novel compounds that are active in maintaining or restoring the balance of the bacterial skin and / or mucosal flora.

Prior Art Documents

Patent Documents

[0015]

Patent Document 1

Patent Document 2

Patent Document 3

Non - Patent Documents

[0016]

Non - Patent Document 1

Non - Patent Document 2

Summary of the Invention

Problems to be Solved by the Invention

[0017] Therefore, there is a need to provide novel components that are active on the microbial skin flora and enable the maintenance and / or restoration of the balance of the bacterial flora of the skin and / or mucosa, particularly by protecting the commensal flora.

Means for Solving the Problems

[0018] Unexpectedly, the inventors have found that a combination of oligosaccharides and / or polysaccharides selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soybean-derived oligosaccharides, pyrodextrin, isomaltoligosaccharides, xylooligosaccharides, transgalactooligosaccharides and mixtures thereof, and mannose monosaccharides, has the ability to maintain and / or restore the balance of the microbial skin and / or mucosal flora by restoring two parameters that decrease particularly in the case of external attack: an appropriate amount of bacteria on the skin (i.e., bacterial load) and the diversity of bacteria on the skin (i.e., alpha and beta diversity).

[0019] Furthermore, this combination enables the prevention and / or treatment of loss of aesthetic qualities of the skin and / or mucous membranes, in particular the prevention and / or treatment of dry skin and / or rough skin.

[0020] "Loss of the aesthetic qualities of the skin" refers to all changes in the skin that have lost their aesthetic appearance, preferably the skin of the face, particularly the cheeks and / or forehead, to the appearance of a particularly rough texture and / or the presence of signs of dryness.

[0021] Furthermore, this combination enables the prevention and / or treatment of sensitive skin, particularly the prevention and / or treatment of sensitive skin discomfort, such as a pulling sensation.

[0022] Therefore, the subject of the present invention is a cosmetic composition for topical application, in a physiologically acceptable medium, - At least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soy-derived oligosaccharides, pyrodextrin, isomaltoligosaccharides, xylooligosaccharides, transgalactooligosaccharides and mixtures thereof, - At least one mannose monosaccharide and It is a cosmetic composition containing [a certain ingredient].

[0023] In particular, the oligosaccharides and / or polysaccharides and the mannose monosaccharide are prebiotics.

[0024] The present invention relates to a method of cosmetic treatment for the care of skin and / or mucous membranes, and also to a method comprising the application of compositions according to the present invention to skin and / or mucous membranes that have been subjected to external attacks.

[0025] In particular, the cosmetic treatment method according to the present invention makes it possible to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0026] Furthermore, the cosmetic treatment method according to the present invention enables the prevention and / or treatment of loss of aesthetic properties of the skin and / or mucous membranes, in particular the prevention and / or treatment of dry skin and / or rough skin.

[0027] The cosmetic treatment method according to the present invention also enables the prevention and / or treatment of sensitive skin, and in particular the prevention and / or treatment of discomfort caused by sensitive skin.

[0028] The present invention also relates to the topical cosmetic use of the aforementioned combination, and optionally at least one probiotic microorganism, for the care of skin, particularly skin subjected to external attacks.

[0029] The present invention also relates to the use of the above combination to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0030] Furthermore, the present invention also relates to the use of the above combination for the prevention and / or treatment of sensitive skin, in particular for the prevention and / or treatment of discomfort of sensitive skin.

[0031] Finally, the present invention also relates to the use of the above combination for the prevention and / or treatment of loss of aesthetic properties of the skin and / or mucous membranes, in particular for the prevention and / or treatment of dry skin and / or rough skin.

[0032] definition The term "prebiotics" refers to substrates that are selectively used by host microorganisms to produce health benefits [Gibson, Glenn R.; Hutkins, Robert; Sanders, Mary Ellen; Prescott, Susan L.; Reimer, Raylene A.; Salminen, Seppo J.; Scott, Karen; Stanton, Catherine; Swanson, Kelly S.; Cani, Patrice D.; Verbeke, Kristin; Reid, Gregor (2017). “Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics”. Nature Reviews Gastroenterology & Hepatology. 14(8):491-502].

[0033] Prebiotics are used to maintain an environment that is particularly favorable to the growth of beneficial endogenous skin flora. Prebiotics are used, in particular, to direct the overall metabolism of skin flora to maintain a good balance of skin flora without promoting the growth of certain pathogenic bacteria. For this purpose, the prebiotics utilized in connection with the present invention can be metabolized by beneficial bacteria of the skin flora. According to one particularly preferred embodiment, the prebiotic used in the composition of the present invention is a prebiotic that is metabolized by beneficial bacteria of the skin but not by undesirable bacteria that cause skin diseases for which cosmetic treatment is desired. According to this embodiment, the prebiotic modulates the composition and / or activity of the natural ecosystem by promoting those microorganisms that have a positive effect on the skin.

[0034] Oligosaccharides and polysaccharides are carbohydrates.

[0035] Oligosaccharides and / or polysaccharides can be produced from glucose, galactose, xylose, maltose, saccharose, lactose, starch, xylan, hemicellulose, inulin, gum, especially acacia gum, or mixtures thereof.

[0036] The term "oligosaccharide" is intended to mean oligoholoside or oligoside. It is an oligomer composed of several n monosaccharides through one or more alpha or beta glycosidic bonds, where n is 2 to 10, preferably 2 to 6. In particular, fructooligosaccharides, glucooligosaccharides, soy-derived oligosaccharides, pyrodextrins, isomaltoligosaccharides, xylooligosaccharides and transgalactooligosaccharides may be described.

[0037] The term "polysaccharide" is intended to mean polyosides, polyholosides, or other complex carbohydrates. These are polymers consisting of several monosaccharide units n linked together by sugar bonds, where n is 11 or greater, preferably 11-200, more preferably 11-100, and even more preferably 20-80. Inulin may be described in particular.

[0038] The phrase "composition containing a physiologically acceptable medium" is intended to mean a composition containing a medium that is compatible with the skin.

[0039] According to one particular embodiment, the pH of the cosmetic composition is 4 to 7.5, particularly 4.5 to 7, and especially 4.7 to 6.

[0040] The term “skin” is intended to mean all skin of the human body, preferably the skin of the face, scalp, neck, arms and forearms, or more preferably the skin of the face, in particular the skin of the forehead, nose, cheeks, chin and around the eyes.

[0041] According to the present invention, the terms “prevent” or “prevention” are intended to mean reducing the risk of a given phenomenon occurring or delaying its occurrence.

[0042] The terms “to treat” or “to cure” are intended to mean any action aimed at improving an individual’s comfort or well-being, and therefore include reducing, mitigating and treating.

[0043] In the context of this invention, the term "external attack" refers to environmental attacks, climatic attacks (heat, cold, UV, tobacco, etc.), pollution, allergens, pathogenic bacteria, mechanical attacks (hair removal, shaving, abrasive cleaning), and chemical attacks (detergents).

[0044] The term "bacterial load" refers to the amount of bacteria, particularly the amount of bacterial DNA found on the skin and / or mucous membranes.

[0045] The term "relative abundance" refers to the proportion of each genus / bacteria in the sample or population under investigation.

[0046] The terms "alpha diversity" or "Shannon index" refer to the number of different bacterial species / genera in a sample or population under investigation. A higher number of different bacterial species / genera indicates a richer and more diverse sample or population.

[0047] In particular, the Shannon index is calculated for each sample or population under investigation and is based on the following three different parameters: - Number of different bacterial species / genera; - In particular, the incidence of the bacteria in question in the sample or population under investigation, regardless of whether the bacteria are present in all individuals of the sample or population, or in only a small number of individuals; - The mass of each bacterial species / genus observed in the sample or population under investigation (the calculation takes into account the relative abundance as defined above).

[0048] The term "beta diversity" refers to the number of different bacterial species / genera between two samples or populations; in other words, the number of bacterial species / genera that are not common to the two samples or populations. Therefore, unlike alpha diversity, beta diversity is calculated based on two samples or populations.

[0049] Beta diversity is based on three different parameters (such as UniFrac distance): - Number of different bacterial species / genera; - Their relative abundance (i.e., the mass of each bacterial species / genus); - Phylogenetic differences between these bacterial species / genera. [Modes for carrying out the invention]

[0050] Oligosaccharides, polysaccharides The composition according to the present invention comprises at least one oligosaccharide and / or polysaccharide selected from the group consisting of inulin, fructooligosaccharides, glucooligosaccharides, soybean-derived oligosaccharides, pyrodextrin, isomaltoligosaccharides, xylooligosaccharides, transgalactooligosaccharides, and mixtures thereof.

[0051] As an example, the following oligosaccharides and / or polysaccharides are listed.

[0052] Inulin Inulin is particularly abundant in plants, especially the rhizomes of Jerusalem artichoke and chicory, from which it is industrially extracted. It is also found in other plants belonging to the Asteraceae family, such as Jerusalem artichoke, dahlia onion, and burdock. It is considered a soluble dietary fiber. [Chemical formula 1] [ka]

[0053] Inulin is a polydisperse linear polymer of general formula (I): GFn (G = glucose, F = fructose, n is in the range of 2 to 60 or more), where the fructose units are linked together by β(2→1) bonds. Therefore, inulin corresponds to a chain of fructose units with glucose units at the ends.

[0054] Among the inulins available for use and commercially available, Inutec H25P (n=2-7) and Inutec N25 (average n=25) from Orafti may be listed.

[0055] Fructooligosaccharides Fructooligosaccharides (or FOS), also called oligofructose or oligofructan, are short chains of fructose linked together by β-1,2 bonds.

[0056] Fructooligosaccharides (or FOS) correspond to the general formula (II): G(F)n (where G is a glucose unit, F is a fructose unit, and n is in the range of 1 to 10). [Chemical formula 2] [ka]

[0057] Fructooligosaccharides (FOS) are - Partial enzymatic hydrolysis of inulin (e.g., Raftilose® from Orafti-Belgique), or - Enzymatic synthesis from saccharose (e.g., Actilight® from Beghin Meiji Industries-France), or - Extraction from yacon or jicama (Polymnia sonchifolia, synonym: Smallanthus sonchifolia) It is produced by, in particular, extraction, which is carried out by room-temperature compression of yacon tubers in the absence of a solvent.

[0058] The FOS used in this invention may be a mixture of FOS. In particular, GF2+GF3+GF4 mixtures such as Quantum FOS95 from Quantum hi-Tech or Actilight® from Beghin Meiji Industries-France may be described, the latter corresponding to a mixture of 37% GF2, 53% GF3, and 10% GF4.

[0059] Gluco-oligosaccharide GOS Gluco-oligosaccharides (GOS) or oligoglucans are oligosaccharides composed of sequences of α-1,6-linked glucose units, which may also contain α-1,2;α-1,3;α-1,4 links. They are synthesized by transglycosylation reactions catalyzed by enzymes of the glucansucrose family.

[0060] Preferably, the gluco-oligosaccharide is an oligosaccharide composed of a sequence of α-1,6- and α-1,2-linked glucose units.

[0061] Advantageously, the number of glucose units is 2 to 10, more preferably 4 to 6, and more preferably 4.

[0062] In addition, gluco-oligosaccharides can be synthesized by polymerization of glucose molecules, catalyzed by specific glycosyltransferase enzymes extracted and purified from bacterial strains of Leuconostoc mesenteroides. This reaction requires the use of an acceptor: maltose (glucose-glucose) and a glucose donor: saccharose (glucose-fructose).

[0063] In a preferred embodiment, the gluco-oligosaccharide (GOS) is of the following formula (III): [Chemical formula 3] [ka] It has.

[0064] Among the GOS that are available and commercially obtainable, Bioecolia (registered trademark) from Solabia may be described.

[0065] Soybean-derived oligosaccharides They are extracted directly from soybeans and do not require any enzymatic treatment. They contain natural raffinose and stachyose, and their formula is [α-D-Gal-(1→6)-] m -α-D-Glu-(1→2)-β-D-Fru (m = 1 for raffinose and m = 2 for stachyose).

[0066] The available derived oligosaccharides include Soya-oligo from Calpis Food Industry Co., Ltd. (Japan).

[0067] Pyrodextrin Pyrodextrin is a mixture of oligosaccharides from the hydrolysis of starch.

[0068] Isomaltooligosaccharides They are produced from starch. These are α-(1,6)-linked glucose oligomers having a degree of polymerization of 2 to 5. As an example, Isomalto900P from Showa Sango may be used.

[0069] Xylooligosaccharides Xylooligosaccharides are oligosaccharides consisting of β-(1,4)-linked xylose. As an example, Xylo 95P from Suntory Limited may be used.

[0070] Transgalactooligosaccharides These are linear oligomers of galactose with the chemical structure α-D-glucose-(1→4)-[β-D-galactose-(1→6)]n (2 < n < 5) obtained by the fermentation of lactose. As an example, TOS 100 from Yakult Honsha Co., Ltd. (Japan) may be used.

[0071] The oligosaccharides and / or polysaccharides according to the present invention are present in the composition according to the present invention at a concentration of 0.01% to 20% by mass, preferably 0.05% to 10% by mass, and more preferably 0.05% to 5% by mass, based on the total mass of the composition (i.e., the total concentration of oligosaccharides and / or polysaccharides in the composition according to the present invention).

[0072] Oligosaccharide and / or polysaccharide mixture According to one preferred embodiment of the present invention, oligosaccharides and / or polysaccharides are used in the form of a mixture in the composition according to the present invention.

[0073] In a first embodiment of the present invention, it may be a mixture of oligosaccharides of the same type. For example, as described above, a mixture of FOS may be used, and in particular a GF2+GF3+GF4 mixture such as Quantum FOS95 from Quantum hi-Tech or Actilight® from Beghin Meiji Industries-France may be used, the latter corresponding to a mixture of 37% GF2, 53% GF3, and 10% GF4.

[0074] According to a second embodiment of the present invention, it may be a mixture of different types of oligosaccharides and / or polysaccharides. The present invention relates in particular to the use of a mixture of inulin with GOS, FOS, soy-derived oligosaccharides, pyrodextrin, isomaltoligosaccharides, xylooligosaccharides and / or transgalactooligosaccharides. According to one particular embodiment, a mixture of inulin with GOS such as Bioline from Gova Ingredients is used. The present invention also relates to the use of a mixture of GOS and FOS.

[0075] Preferably, in this second embodiment, the composition according to the present invention is - At least one gluco-oligosaccharide (GOS), - At least one fructooligosaccharide (FOS) and The composition contains a mixture of oligosaccharides, wherein the gluco-oligosaccharide (GOS) is present in the composition according to the present invention at a concentration of 0.01% to 10% by mass, preferably 0.05% to 5% by mass, more preferably 0.1% to 1% by mass, and even more preferably 0.2% to 0.8% by mass, based on the total mass of the composition. The fructooligosaccharide (FOS) is present in the composition according to the present invention at a concentration of 0.001% to 5% by mass, preferably 0.01% to 1% by mass, more preferably 0.01% to 0.5% by mass, and even more preferably 0.05% to 0.3% by mass, based on the total mass of the composition.

[0076] Advantageously, in a second embodiment of the present invention, the composition according to the present invention is - At least one gluco-oligosaccharide (GOS), - At least one fructooligosaccharide (FOS) and The mixture contains oligosaccharides, and the [GOS / FOS] mass ratio is at least 2, preferably 2-4, and more preferably 3-4.

[0077] A mixture of prebiotic oligosaccharides and probiotic microorganisms sold by Solabias under the name Ecoskin® may be particularly used.

[0078] The aforementioned mixture, in particular, - At least one gluco-oligosaccharide (GOS) in an amount of 60% to 80% by mass relative to the total mass of the mixture, - At least one fructooligosaccharide (FOS) in an amount of 10% to 25% by mass relative to the total mass of the mixture Includes.

[0079] In particular, the mixture contains 70% by mass of gluco-oligosaccharide (GOS), 19% by mass of yacon (Polymnia sonchifolia) tuber juice, 1% by mass of Lactobacillus acidophilus and Lactobacillus casei, and 10% by mass of maltodextrin.

[0080] Probiotics In addition to the two variants described above, the composition according to the present invention may also contain at least one probiotic microorganism.

[0081] For the purposes of this invention, the term “probiotic microorganism” is intended to mean a living microorganism that, when consumed in appropriate amounts, has a positive effect on the health of its host ("Joint FAO / WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotic in Food Including Powder Milk with Live Lactic Acid Bacteria, 6 October 2001") and, in particular, can improve the balance of intestinal microbiota.

[0082] In the case of skin, the probiotic microorganisms are those that, when applied to the skin in appropriate amounts, have a positive effect on the aesthetic properties of the skin and mucous membranes.

[0083] More specifically, these are probiotic microorganisms from the group of lactic acid bacteria, particularly those of the genus Lactobacillus. Examples of these lactic acid bacteria include, more specifically, Lactobacillus casei, Lactobacillus acidophilus, and mixtures thereof.

[0084] Specific examples of probiotic microorganisms include Lactobacillus acidophilus, Lactobacillus alimentarius, Lactobacillus curvatus, Lactobacillus delbruckii subsp. Lactis, Lactobacillus gasseri, Lactobacillus johnsonii, Lactobacillus reuteri, Lactobacillus casei, Lactobacillus rhamnosus (Lactobacillus GG), Lactobacillus salmon Lactobacillus, Lactococcus lactis, Streptococcus thermophilus, Lactobacillus acidophilus, L. delbrueckii, L. helveticus, L. salivarius, L. curvatus, L. plantarum, L. sakei, L. brevis, L. buchneri, L. fermentum, L. reuteri, Lactobacillus bulgaricus, Lactobacillus longum These include Lactobacillus lactis, Bifidobacterium longum, and mixtures thereof.

[0085] Generally, the composition according to the invention generally contains at least one probiotic microorganism in an amount of 0.0001 to 20% by weight, based on the total weight of the composition.

[0086] Advantageously, the probiotic microorganism is present in the composition according to the invention at a concentration of 0.0001% to 10% by weight, preferably 0.001% to 5% by weight, even more preferably 0.001% to 1% by weight, based on the total weight of the composition.

[0087] The microorganisms can be included in the composition according to the invention in a viable, semi-active or inactive, dead state.

[0088] They can also be included in the form of a fraction of cell components or in the form of metabolites. The microorganisms, metabolites or fractions can be introduced in the form of freeze-dried powder, culture supernatant and / or, where appropriate, in a concentrated form.

[0089] According to one preferred embodiment of the invention, these microorganisms are in an inactive form, in particular inactivated by heat or high pressure.

[0090] The terms "inactive form", "non-renewable form" and "dead form" are synonymous herein.

[0091] "Semi-active" bacteria are bacteria that have partially or completely lost their growth characteristics.

[0092] When the microorganisms are in a viable form and incorporated into the composition, the amount of live microorganisms can range from 10 3 to 10 15 cfu / g, particularly from 10 5 to 10 15 cfu / g, more particularly from 10 7 to 10 12 cfu / g per gram of the composition.

[0093] In one particularly preferred embodiment, the composition according to the invention - At least one gluco-oligosaccharide (GOS), - At least one fructooligosaccharide (FOS) and The composition contains a mixture of oligosaccharides, wherein the gluco-oligosaccharide (GOS) is present in the composition according to the present invention at a concentration of 0.01% to 10% by mass, preferably 0.05% to 5% by mass, more preferably 0.1% to 1% by mass, and even more preferably 0.2% to 0.8% by mass, based on the total mass of the composition. The fructooligosaccharide (FOS) is present in the composition according to the present invention at a concentration of 0.001% to 5% by mass, preferably 0.01% to 1% by mass, more preferably 0.01% to 0.5% by mass, and even more preferably 0.05% to 0.3% by mass, based on the total mass of the composition. The composition according to the present invention also includes, in addition to 0.0001% to 10% by mass, preferably 0.001% to 5% by mass, and more preferably 0.001% to 1% by mass, a probiotic microorganism selected from bacteria of the genus Lactobacillus, particularly Lactobacillus casei, Lactobacillus acidophilus, and mixtures thereof, based on the total mass of the composition.

[0094] Advantageously, the compositions according to the present invention are - At least one gluco-oligosaccharide (GOS), - At least one fructooligosaccharide (FOS) and The mixture contains oligosaccharides, and the [GOS / FOS] mass ratio is at least 2, preferably 2-4, and more preferably 3-4.

[0095] A mixture of prebiotic oligosaccharides and probiotic microorganisms sold by Solabias under the name Ecoskin® may be particularly used.

[0096] The aforementioned mixture, in particular, - At least one gluco-oligosaccharide (GOS) in an amount of 60% to 80% by mass relative to the total mass of the mixture, - At least one fructooligosaccharide (FOS) in an amount of 10% to 25% by mass relative to the total mass of the mixture, - 0.001% to 15% by mass of at least one probiotic microorganism selected from Lactobacillus casei, Lactobacillus acidophilus, and mixtures thereof, relative to the total mass of the mixture. Includes.

[0097] In particular, the mixture contains 70% by mass of gluco-oligosaccharide (GOS), 19% by mass of yacon (Polymnia sonchifolia) tuber juice, 1% by mass of Lactobacillus acidophilus and Lactobacillus casei, and 10% by mass of maltodextrin.

[0098] Mannose The composition according to the present invention contains mannose monosaccharide.

[0099] Mannose is a monosaccharide (simple, non-hydrolyzable sugar) consisting of six carbon atoms; it is a hexose. Its empirical formula is the same as glucose: C6H 12 It is O6 and a C2 epimer (i.e., its spatial configuration is exactly the same as glucose, except for the substituent on carbon 2, which is the reverse of that).

[0100] The monosaccharide in question according to the present invention corresponds to the following formula (IV). [Chemical formula 4] [ka]

[0101] Naturally, all enantiomers of mannose are taken into consideration in this invention.

[0102] Mannose can exist in solvated form (including hydrates), or in the form of a mixture of D and L stereoisomers: DL-mannose.

[0103] According to one preferred embodiment, the mannose monosaccharide is D-mannose of the following formula (V): [Chemical formula 5] [ka]

[0104] D-mannose is naturally present in plants, particularly in certain fruits including cranberries, or in hardwoods (beech and birch).

[0105] Examples of suitable D-mannose for the present invention include D-mannose sold by Danisco Sweeteners® or Symrise®.

[0106] Generally, mannose monosaccharide can be used in an amount of 0.01% to 20% by mass, preferably 0.05% to 10% by mass, and most preferably 0.1% to 5% by mass, relative to the total mass of the composition containing it.

[0107] As described above, the compositions according to the present invention include a physiologically acceptable medium. More specifically, the physiologically acceptable medium may include one or more water-soluble organic solvents that can be selected from water and / or linear or branched C1-C6 monoalcohols such as ethanol, isopropanol, tert-butanol or n-butanol; polyols such as glycerol, propylene glycol, hexylene glycol (or 2-methyl-2,4-pentanediol) and polyethylene glycol; polyol ethers such as dipropylene glycol monomethyl ether; and mixtures thereof.

[0108] Preferably, the composition according to the present invention has a water content in the range of 20% to 95% by mass, and more preferably 40% to 90% by mass, based on the total mass of the composition.

[0109] Advantageously, the composition contains one or more water-soluble organic solvents in an amount ranging from 0.5% to 25% by mass, preferably 5% to 20% by mass, and more preferably 10% to 15% by mass, relative to the total mass of the composition.

[0110] Presentation form The cosmetic composition according to the present invention is applied topically.

[0111] Compositions intended for external topical administration may be aqueous, aqueous-alcoholic, or oily solutions, lotions, or whey-type solutions or dispersions, milk-type liquid or semi-liquid-viscosity emulsions obtained by dispersion of a fatty phase in an aqueous phase (O / W) or vice versa (W / O), or cream-type soft, semi-solid, or solid-viscosity suspensions or emulsions, aqueous or anhydrous gels, microemulsions, microcapsules, fine particles, or ionic and / or nonionic porous dispersions.

[0112] These compositions are prepared by conventional methods.

[0113] The topical compositions according to the present invention can be advantageously formulated in any of the presented forms suitable for skincare, particularly in the form of a cream for cleansing, protecting, treating or caring for the face or body, in the form of a mask left on the skin or hair, in the form of a makeup removal milk, in the form of a body protection or care milk, or in the form of a lotion, gel or foam for skincare, such as a cleansing lotion or a bath additive composition.

[0114] Alternatively, the topical compositions according to the present invention may be advantageously formulated in any of the presented forms suitable for hair care, particularly in the form of a hair lotion, a shampoo, particularly in the form of an anti-dandruff shampoo, a conditioner, a disentangler, a hair cream or gel, a treatment lotion, a hair loss prevention lotion or gel, an antiparasitic shampoo or a treatment shampoo, particularly in the form of an anti-seborrheic shampoo, a scalp care product, particularly in the form of an anti-irritant, anti-aging, or constitution-improving product.

[0115] Supplement In known methods, the presentation form intended for topical administration may also contain adjuvants common in the fields of cosmetic, pharmaceutical, and / or dermatology, such as hydrophilic or lipophilic gelling agents, hydrophilic or lipophilic surfactants, preservatives, antioxidants, solvents, fragrances, fillers, screening agents, fatty substances such as oils, emulsifiers, odor absorbers, and colorants. The amounts of these various adjuvants are, for example, amounts from 0.01% to 20% of the total mass of the composition, as is customary in the field under consideration. Depending on their properties, these adjuvants may be introduced into the fatty phase and / or aqueous phase.

[0116] Examples of hydrophilic gelling agents usable in the compositions according to the present invention include modified or unmodified carboxyvinyl polymers, such as products sold by Goodrich under the names Carbopol (INCI name: carbomer) and Pemulen (INCI name: acrylate / C10-30 alkyl acrylate crosspolymer) or crosslinked sodium polyacrylate sold by Cognis under the name Cosmedia SP (INCI name: sodium polyacrylate); polyacrylamide; 2-acrylamide-2-methylpropanesulfonic acid homopolymer, such as products sold by Clariant under the name Hostacerin AMPS (INCI name: ammonium polyacryldimethyltaurate); crosslinked anion copolymers of acrylamide and AMPS provided in emulsion form, such as Sepigel 305 (CTFA name: polyacrylamide / C13-14 isoparaffin / Laureth-7) and Simulgel sold by SEPPIC. Products marketed under the name 600 (CTFA name: acrylamide / sodium acryloyldimethyl taurate copolymer / isohexadecane / Polysorbate 80); acrylate / acrylonitrile copolymers, e.g., Hypan SS201 marketed by Kingston; synthetic neutral polymers, e.g., poly-N-vinylpyrrolidone; polysaccharides, e.g., guar gum, xanthan gum, and cellulosic derivatives may be described. The amount of these polymers may range from, for example, 0.05% to 5% by mass, and more preferably from 0.1% to 3% by mass, relative to the total mass of the composition.

[0117] Examples of lipophilic gelling agents that may be described include modified clays, such as benton, metal salts of fatty acids, such as aluminum stearate, and hydrophobic silica, or alternatively, ethyl cellulose and polyethylene.

[0118] When the composition of the present invention is an emulsion, the proportion of the fatty phase may be in the range of 5% to 80% by mass, preferably 10% to 50% by mass, based on the total mass of the composition. The oils, emulsifiers, and co-emulsifiers used in the composition in emulsion form are selected from those conventionally used in cosmetics and / or dermatology. The emulsifiers and co-emulsifiers may be present in the composition in the range of 0.3% to 30% by mass, preferably 0.5% to 20% by mass, based on the total mass of the composition.

[0119] When the composition of the present invention is an oily solution or gel, the fatty phase may constitute 90% or more of the total mass of the composition.

[0120] Examples of oils that may be used include the following: - Plant-derived hydrocarbon oils, such as liquid fatty acid triglycerides containing 4 to 10 carbon atoms, such as heptanoic acid or octanoic acid triglycerides, or such as sunflower oil, corn oil, soybean oil, mallow oil, grapeseed oil, sesame seed oil, hazelnut oil, apricot oil, macadamia oil, arara oil, castor oil, avocado oil, caprylic / capric acid triglycerides, such as those sold by Stearineries Dubois or by Dynamit Nobel under the names Miglyol 810, 812, and 818, jojoba oil, and shea butter oil; - In particular, synthetic esters and ethers of fatty acids, for example, oils of formulas R1COOR2 and R1OR2 (wherein R1 represents a fatty acid residue containing 8 to 29 carbon atoms, and R2 represents a branched or unbranched hydrocarbon chain containing 3 to 30 carbon atoms), for example, purcellin oil, isononyl isononanoate, isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, 2-octyldodecyl erucate, or isostearyl isostearate; hydroxyl Esters, such as isostearyl lactate, octyl hydroxystearate, octyldodecyl hydroxystearate, diisostearyl malate, triisocetyl citrate, and fatty alcohols heptanoates, octanoates, and decanoates; polyol esters, such as propylene glycol dioctanoate, neopentyl glycol diheptanoate, and diethylene glycol diisononanoate; and pentaerythritol esters, such as pentaerythrityl tetraisostearate; - Mineral or synthetic linear or branched hydrocarbons, such as liquid paraffins and their derivatives which may be volatile or nonvolatile, petrolatum, polydecene, hydrogenated polyisobutene, such as pearlleam oil, fatty alcohols having 8 to 26 carbon atoms, such as cetyl alcohol, stearyl alcohol and mixtures thereof (cetylstearyl alcohol), octyldodecanol, 2-butyloctanol, 10 2-Hexyldecanol, 2-Undecylpentadecanol, Oleyl alcohol or Linoleyl alcohol; Silicone oils, e.g., volatile or non-volatile polymethylsiloxanes (PDMS) having linear or cyclic silicone chains, which are liquid or paste at ambient temperature, especially cyclopolydimethylsiloxane (cyclomethicone), e.g., cyclohexasiloxane; Polydimethylsiloxanes containing alkyl, alkoxyl or phenyl groups having 2 to 24 carbon atoms at the pendant group or silicone chain end; Phenylated silicones, e.g., phenyl trimethicone, phenyl dimethicone, phenyl trimethylsiloxydiphenylsiloxane, diphenyl dimethicone, diphenylmethyldiphenyltrisiloxane, 2-phenylethyltrimethylsiloxysilicate and polymethylphenylsiloxane; and - A mixture of those.

[0121] In the list of oils above, the term "hydrocarbon oil" refers to any oil that contains primarily carbon and hydrogen atoms, and optionally ester, ether, fluoro, carboxylic acid, and / or alcohol groups.

[0122] Emulsifiers may include amphoteric, cationic, anionic, or nonionic surfactants, which are used alone or optionally in combination with coemulsifiers in mixtures.

[0123] With respect to O / W emulsions, for example, nonionic emulsifiers such as oxyalkylene (more particularly polyoxyethylene) fatty acid esters of glycerol; oxyalkylene fatty acid esters of sorbitan; oxyalkylene (oxyethylene and / or oxypropylene) fatty acid esters; oxyalkylene (oxyethylene and / or oxypropylene) fatty alcohol ethers; sugar esters, particularly oxyalkylene sugar esters, esters of phosphoric acid and fatty alcohols; and mixtures thereof may be described.

[0124] In one preferred embodiment, the composition according to the present invention includes silicone fatty substances such as silicone oils, gums and waxes; non-silicone fatty substances such as oils, pastes and waxes of plant, mineral, animal and / or synthetic origin; fatty acids having 8 to 32 carbon atoms; in particular, formula R 1 COOR 2 and R 1 Ure 2 (In the formula, R 1 represents a fatty acid residue containing 8 to 29 carbon atoms, and R 2 The following are examples of compounds containing a polymer: synthetic esters and ethers of branched or unbranched hydrocarbon chains containing 3 to 30 carbon atoms; linear or branched hydrocarbons of mineral or synthetic origin; fatty alcohols having 8 to 26 carbon atoms; water; C2-C6 monoalcohols; glycols selected from propylene glycol, butylene glycol, and pentylene glycol; ketones; and at least one component selected from thickeners, emulsifiers, surfactants, gelling agents, fragrances, fillers, colorants, moisturizers, vitamins selected from vitamins A, E, and B3.

[0125] The amounts of these various components are, for example, amounts ranging from 0.01% to 20% of the total mass of the composition, which are conventionally used in the field under consideration.

[0126] Cosmetic treatments and cosmetic uses The present invention relates to a method for the cosmetic care treatment of the skin and / or mucous membranes, and also to a method comprising the application of a composition according to the present invention to skin and / or mucous membranes that have been subjected to external attacks.

[0127] In particular, the cosmetic treatment method according to the present invention makes it possible to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0128] Furthermore, the cosmetic treatment method according to the present invention enables the prevention and / or treatment of loss of aesthetic properties of the skin and / or mucous membranes, in particular the prevention and / or treatment of dry skin and / or rough skin.

[0129] The cosmetic treatment method according to the present invention also enables the prevention and / or treatment of sensitive skin, and in particular the prevention and / or treatment of discomfort caused by sensitive skin.

[0130] The present invention also relates to topical cosmetic use of the above-defined combination, and optionally, at least one of the above-defined probiotic microorganisms, for the care of skin, particularly skin subjected to external attacks.

[0131] The present invention also relates to the use of the above combination to maintain and / or restore the balance of the bacterial flora of the skin and / or mucous membranes.

[0132] Furthermore, the present invention also relates to the use of the above combination for the prevention and / or treatment of sensitive skin, in particular for the prevention and / or treatment of discomfort of sensitive skin.

[0133] Regarding discomfort, these may typically be sensations of itching, heat or stinging and / or pulling. Representative visible skin signs that may be specifically described include itching, dry patches, erythema and / or redness.

[0134] These phenomena are more common in most exposed areas of the body, namely the hands, feet, face, and scalp.

[0135] Therefore, the appearance of unpleasant symptoms that occur a few minutes after individual contact with the causative factor is one of the essential characteristics of sensitive skin. This is mainly associated with dasteric sensations, which are sensory nerve signs. Dasteric sensations are understood to be sensations such as stinging, burning, itching or itching, heat, discomfort, and pulling. These subjective symptoms usually exist in the absence of visible clinical signs such as desquamation. It is now known that these skin intolerance reactions are associated with the release of neuropeptides by nerve endings, particularly in the epidermis and dermis.

[0136] Furthermore, the present invention relates to preventing and / or treating the signs of diasteric sensation in sensitive skin.

[0137] The present invention proposes to prevent and / or reduce skin discomfort sensations such as stinging, burning, itching or itching, heat, discomfort, erythema and / or tightness, particularly in people with sensitive skin.

[0138] In contrast to skin recognized as "allergic," the reactivity of sensitive skin is not an immunological process; that is, it does not arise in response to the presence of an allergen.

[0139] Furthermore, its response mechanism is said to be "non-specific." In this respect, it differs from skin exhibiting inflammatory and allergic reactions such as dermatosis, eczema, and / or ichthyosis.

[0140] For the purposes of this invention, the term "sensitive skin" includes hypersensitive skin and intolerant skin.

[0141] In relation to the present invention, the sensitive skin under consideration does not exhibit any inflammatory properties.

[0142] Finally, the present invention also relates to the use of the above combination for the prevention and / or treatment of loss of aesthetic properties of the skin and / or mucous membranes, in particular for the prevention and / or treatment of dry skin and / or rough skin.

[0143] Dry skin essentially manifests as an unpleasant sensation, such as a feeling of tension and / or stiffness. Such dry skin may feel rough to the touch and / or appear scaly. When the skin is only slightly dry, the scales are numerous but barely visible to the naked eye. They gradually become less numerous, but as the condition worsens, they become more visible to the naked eye.

[0144] The cause of this dry skin can be congenital or acquired.

[0145] The aforementioned combination is preferably intended to treat non-pathological congenital dry skin or non-pathological acquired dry skin.

[0146] In cases of acquired dry skin, external parameters such as exposure to chemical agents, harsh weather conditions or sunlight, or other specific treatments (e.g., retinoids) are determinants. These external influences can cause the skin to dry out momentarily and locally.

[0147] Non-pathological congenital dry skin is dry skin whose severity may depend on the external factors already indicated. This skin category includes aged skin (characterized by a general decrease in skin metabolism with age), delicate skin (highly sensitive to external factors and often accompanied by erythema), and typical xerosis (often due to genetic causes and primarily appearing on the face, hands, feet, and backs of the hands).

[0148] The following examples illustrate the present invention and are provided purely as non-limiting examples.

[0149] Throughout the following text, unless otherwise stated, percentages are given on a mass basis.

[0150] The phrase "at least one" is synonymous with "one or more."

[0151] Expressions such as "...~..." and "...~...", "at least...", or "at most" should be understood as comprehensive limitations unless otherwise specified.

[0152] The following embodiments and drawings are provided as illustrative examples, not limiting the field of the present invention. [Examples]

[0153] Example 1 - Evaluation of the recovery of skin bacterial flora after application of the composition according to the present invention following the use of an aggressive cleanser. A group of 30 women aged 30-50 was selected. In this study, the subjects' facial skin was cleansed using an aggressive cleanser.

[0154] The cleanser composition contained water, triethanolamine, glycerin, myristic acid, lauric acid, cocamidopropyl betaine, lauryl phosphate, hydroxypropyl methylcellulose, pentasodium triphosphate, O-cymen-5-ol (isopropylmethylphenol), benzophenone-4, and butylhydroxytoluene.

[0155] The skin of the subject's cheek was evaluated as follows: - Bare skin (D0): T0be, T0af, T3h and T6h; - Immediately after application of composition 1 (D5): T3h and T6h; - 14 days after treatment with composition 1 (D19): T3h and T6h.

[0156] T0be: Before cleansing; T0af: Immediately after cleansing; T3h: 3 hours after cleansing; T6h: 6 hours after cleansing.

[0157] The evaluation will be conducted based on the following criteria: Roughness: - Roughness was assessed by a clinical score: clinicians assessed skin roughness to touch without touching the cheek area where sampling was performed; - The scale is 0 to 4, as follows: 0 = Absent: A perfectly smooth and resilient surface. 1 = Mild: Slight irregularity and roughness when in contact in the tangential direction. 2 = Moderate: Significant skin irregularity, rough appearance, and slight hardening detected by vertical contact. 3 = Severe: More pronounced irregularity and roughness combined with hardening of the skin. 4 = Extreme: Major disorder of skin pattern due to very pronounced irregularities and more pronounced signs.

[0158] Drying: - Dryness was assessed by a clinical score: clinicians investigated dryness of the facial skin. - The scale is 0 to 3, as follows: 0 = Skin that is not dry 1 = Mildly dry (mildly rough) 2 = Moderately dry (moderate roughness, some shedding) 3 = Severe dryness (extremely rough and severely flaky).

[0159] Discomfort: - The sensation of discomfort (stinging, itching, heat, pulling) was assessed by self-evaluation: The interviewer asked volunteers to perform self-evaluations immediately after intensive cleansing and during kinetics at 3 and 6 hours later, using the following scale from 0 to 4: 0 = None at all 1 = Mild: 2=moderate: 3=Severe: 4 = Very severe.

[0160] Analysis of bacterial load and bacterial population: - Total bacterial load was assessed by QPCR (QPCR on V1-V3 16S rRNA using the SYBER Green n triple (primer set 27F / 534R) KAPA Biosystems kit). - Metagenomic analysis of bacterial populations was performed using next-generation sequencing (V1-V3 growth on the Illumina MiSeq platform (primer set 27F / 534R)), taxonomic assignment was performed using SILVA bases, and OTU was analyzed by QIIME (open reference), thus making it possible to calculate alpha diversity (Shannon index) and beta diversity (UniFrac distance).

[0161] The following composition 1 according to the present invention was prepared.

[0162] [Table 1]

[0163] Composition 1 is prepared as follows: The aqueous phase components are homogenized while stirring, and the mixture is heated to a temperature of 60°C. The fatty phase components, which have been pre-mixed and heated to a temperature of 60°C, are added to this heated mixture. Then, the gelling agent is added. The mixture is kept under stirring until emulsification occurs, and the mixture is cooled to a temperature of 22°C to 23°C while continuously stirring.

[0164] Composition 1 was applied to the entire face.

[0165] Evaluation of skin roughness

[0166] [Table 2]

[0167] The values ​​listed in Table 2 above represent the average (n=30) skin roughness scores evaluated using the scale detailed above.

[0168] Conclusion: Using the area under the curve, 14 days after treatment (D19), the application of composition 1 according to the present invention shows a significant reduction in skin roughness compared to the absence of the composition (p<0.001).

[0169] Evaluation of skin dryness

[0170] [Table 3]

[0171] The values ​​listed in Table 3 above represent the average (n=30) skin dryness scores evaluated using the scale detailed above.

[0172] Conclusion: - Using the area under the curve, 14 days after treatment (D19), the application of composition 1 according to the present invention showed a significant reduction in skin dryness compared to the non-application of the composition (p=0.0001). - Fourteen days after treatment (D19), the application of composition 1 according to the present invention showed a significant reduction in skin dryness compared to the single application of the composition on D5 (p<0.0001).

[0173] Evaluation of discomfort

[0174] [Table 4]

[0175] The values ​​reported in Table 4 above represent the mean (n=30) of skin discomfort scores assessed using the scale detailed above.

[0176] Conclusion: - Using the area under the curve, the application of composition 1 according to the present invention shows a significant reduction in skin discomfort after single application at D5 compared to no application of the composition (p<0.001). - Furthermore, 14 days after treatment (D19), the application of composition 1 according to the present invention showed a significant reduction in skin discomfort compared to the single application of the composition on D5 (p<0.001). - Finally, 14 days after treatment (D19), the application of composition 1 according to the present invention showed a significant reduction in skin discomfort compared to the absence of the composition (p<0.001).

[0177] Evaluation of the common logarithmic bacterial load

[0178] [Table 5]

[0179] The values ​​reported in Table 5 above represent the average (n=30) of bacterial load using the evaluation method detailed above.

[0180] Conclusion: - Using the area under the curve, the application of composition 1 according to the present invention shows a significant increase in bacterial load after single application at D5 compared to the absence of the composition (p<0.001). - Furthermore, 14 days after treatment (D19), the application of composition 1 according to the present invention showed a significant increase in bacterial load compared to the absence of the composition (p<0.001), indicating complete recovery of the bacterial load.

[0181] Evaluation of Alpha Diversity (Shannon Index)

[0182] [Table 6]

[0183] The values ​​reported in Table 6 above represent the central alpha diversity using the evaluation method described in detail above.

[0184] conclusion - A significant decrease in alpha diversity was observed after cleansing (p<0.05). In bare skin where a significant decrease in diversity (p<0.05) was observed, no recovery was seen 3 hours and 6 hours after cleansing. - The initial application of composition 1 according to the present invention in D5 indicated the start of alpha diversity recovery (p=0.024), signaling the beginning of recovery. - After 14 days (D19), the application of composition 1 according to the present invention showed a significant increase in alpha diversity, indicating a recovery of alpha diversity. The diversity at T6h after cleansing was no different from that at T0 before cleansing (p>0.05), which is an indicator of complete recovery of alpha diversity. - Furthermore, 14 days later (D19), the application of composition 1 according to the present invention showed a significantly different recovery of alpha diversity compared to the absence of the composition (p=0.023).

[0185] Beta diversity / UniFrac distance evaluation - The value R represents the phylogenetic distance compared to T0 before cleansing.

[0186] [Table 7]

[0187] The values ​​reported in Table 7 above represent the average (n=30) of beta diversity using the evaluation method detailed above. A larger decrease in the value R indicates a smaller phylogenetic distance compared to T0 before cleansing, and greater recovery performance of the bacterial population.

[0188] Conclusion: - Cleansing with aggressive cleansers causes significant alteration of the structure of the skin's bacterial population. It is important to note that the changes after T3h are greater than those at T0 after cleansing. This means that skin bacteria are difficult to restore. - Compared to untreated cases, lower R values ​​have already been observed after the application of Composition 1 according to the present invention alone, which indicates the initiation of recovery. - 14 days of application of composition 1 according to the present invention led to smaller changes immediately after cleansing, and moreover, a lower R value was also obtained compared to single application: 3 hours after cleansing. The R value was very low, and the value p was no longer significantly different, suggesting that the bacterial population was similar to that before cleansing and indicating an overall recovery of bacterial beta diversity.

[0189] Example 2 - Comparison of post-application skin bacterial flora recovery between compositions according to the present invention and compositions other than those according to the present invention after use of an aggressive cleanser. A group of 30 women aged 55-65 was selected. In this study, the facial skin of the subjects was cleansed using an aggressive cleanser. The protocol applied was the same as that described in Example 1 above, except that composition 1 was replaced with compositions 2 (according to the present invention) and 3 (not according to the present invention).

[0190] The cleanser composition contained water, triethanolamine, glycerin, myristic acid, lauric acid, cocamidopropyl betaine, lauryl phosphate, hydroxypropyl methylcellulose, pentasodium triphosphate, O-cymen-5-ol (isopropylmethylphenol), benzophenone-4, and butylhydroxytoluene.

[0191] The skin of the subject's cheek was evaluated as follows: - Bare skin (D0): T0be, T0af, T3h and T6h; - Immediately after application of the composition (D3): T3h and T6h; - 15 days after treatment with the composition (D18): T3h and T6h.

[0192] T0be: Before cleansing; T0af: Immediately after cleansing; T3h: 3 hours after cleansing; T6h: 6 hours after cleansing.

[0193] The evaluation will be conducted based on the following criteria. - tension sensation - Bacterial load - Alpha diversity (Shannon index) - Beta diversity

[0194] The score scale used is the same as that shown in Example 1.

[0195] The following compositions 2 and 3 (other than the present invention) were prepared.

[0196] [Table 8]

[0197] Compositions 2 and 3 were prepared as follows: Since the compounds are insoluble at cold temperatures, they were mixed and heated to 80°C to form a pre-emulsion. Compositions 2 and 3 were then cooled to 35°C. After that, a gelling agent was added to dissolve them. The probiotic fraction was added at ambient temperature.

[0198] Compositions 2 and 3 were applied to the entire face.

[0199] Evaluation of tensile sensation

[0200] [Table 9]

[0201] [Table 10]

[0202] Conclusion: From the first application of compositions 2 and 3, a significantly more rapid reduction in the tensile sensation induced by aggressive cleansers was observed compared to bare skin (D0) (p<0.001). Fifteen days after application of compositions 2 and 3, compared to bare skin, they provided significant protection against the tensile sensation induced by aggressive cleansers (p<0.001).

[0203] Assessment of bacterial load

[0204] [Table 11]

[0205] [Table 12]

[0206] Conclusion: With the application of two compositions 2 and 3, bacterial load can be recovered significantly more rapidly and completely from 3 hours (p<0.0001); this effectiveness was also confirmed 15 days after application, where the two formulations enabled significantly more complete and rapid recovery of total cellular load from 3 hours (p<0.0001).

[0207] Evaluation of Alpha Diversity (Shannon Index)

[0208] [Table 13]

[0209] The values ​​reported in Table 13 (Composition 3) above represent the average alpha diversity evaluated by the method detailed above.

[0210] The recovery observed at D3 was better, but there was no significant difference compared to D0, and no significant difference in the recovery of bacterial diversity was observed between D0 (bare skin), D3 (single application), and D18 (14 days after application). Therefore, there was no better recovery compared to bare skin.

[0211] [Table 14]

[0212] The values ​​reported in Table 14 (Composition 2 according to the present invention) above represent the average alpha diversity evaluated by the method detailed above.

[0213] Compared with D0(T6h), a significant recovery is observed at D18(T6h) (p < 0.005). 15 days after use, the bacterial skin diversity rapidly recovers 2.73 times after the attack. The recovery is greater and more rapid at D18 compared to the bare skin at D0 and compared to a single application of Composition 2 at D3.

[0214] Evaluation of beta diversity - The value R represents the phylogenetic distance compared to T0 before cleansing.

[0215]

Table 15

[0216] The values reported in Table 15 above (Composition 3) represent the average (n = 30) of beta diversity evaluated by the method detailed above. The greater the decrease in the value R, the smaller the phylogenetic distance compared to T0 before cleansing and the greater the recovery performance of the bacterial population.

[0217] Based on the reference data at T0 before cleansing (bare skin), an increase in the R value is seen. This indicates the change and distance of the bacterial population. At D3 and D18, the value R decreased but remained significantly different compared to D0, again indicating the change and lack of recovery of the bacterial population at D0, D3, and D18.

[0218]

Table 16

[0219] The values reported in Table 16 above (Composition 2) represent the average (n = 30) of beta diversity evaluated by the method detailed above. The greater the decrease in the value R, the smaller the phylogenetic distance compared to T0 before cleansing and the greater the recovery performance of the bacterial population.

[0220] Based on reference data from before cleaning, no recovery is observed at D0. The R value remains substantially unchanged between after cleaning (R=0.071) and T6h (R=0.057).

[0221] No recovery was observed in D3. The R value remained virtually unchanged between immediately after washing (R=0.097) and T6h (R=0.079).

[0222] A rapid recovery is observed on D18. The R value decreases rapidly after cleansing (R=0.100), T3h (R=0.036), and T6h (R=0.018). Significant full recovery is observed from T3h to D18.

[0223] General conclusion: Compositions 2 (according to the present invention) and 3 (other than the present invention) enable a reduction in the sensation of tension.

[0224] Only composition 2 according to the present invention, which contains a combination of oligosaccharide and mannose, allows for a more rapid and substantial recovery in alpha and beta diversity after the use of an aggressive cleanser.

[0225] References: [1]Lai Y, Di Nardo A, Nakatsuji T, et al.Commensal bacteria regulate Toll-like receptor 3-dependent inflammation after skin injury.Nature Medicine 2009,15:1377-1382 [2] Nakatsuji T, Gallo RL. Antimicrobial Peptides: Old Molecules with New Ideas J Invest Dermatol 2012,132,3:887-895 [3]Yuki T,Yoshida H,Akazawa Y et al.Activation of TLR2 Enhances Tight Junction Barrier in Epidermal Keratinocytes.J Immunol 2011,187,6,3230-3237 [4]Byrd AL,Belkaid Y,Segre JA.The human skin microbiome.Nature Reviews Microbiology 2018,16:143-155 [5]Stacy A,Belkaid Y.Microbial guardians of skin health.Sciences 2019,363,6424:227-228 [6]Linehan JL,Harrison OJ,Seong-JI et al.Non-classical Immunity Controls Microbiota Impact on Skin Immunity and Tissue Repair.Cell 2018,172:784-796 [7]Lee SE,Kim JM,Jeong SK,et al.Protease-activated receptor-2 mediates the expression of inflammatory cytokines,antimicrobial peptides,and matrix metalloproteinases in keratinocytes in response to Propionibacterium acnes.Archives of Dermatol Res 2010,302,10,745-756 [8]Iinuma K,Sato T,Akimoto N,et aI.Involvement of Propionibacterium acnes in the Augmentation of Lipogenesis in Hamster Sebaceous Glands In Vivo and In Vitro.J Invest Dermatol 2009,129,9:2113-2119 [9]Allhorn M,Arve S,Brueggemann H et al.A novel enzyme with antioxidant capacity produced by the ubiquitous skin colonizer Propionibacterium acnes.Sci Rep.2016;6:36412

Claims

1. A cosmetic composition for topical application, in a physiologically acceptable medium, - At least one oligosaccharide and / or polysaccharide, and - At least one mannose monosaccharide Includes, The oligosaccharide and / or polysaccharide is in the form of a mixture containing at least one gluco-oligosaccharide (GOS) and at least one fructo-oligosaccharide (FOS), The aforementioned gluco-oligosaccharide (GOS) is present in the composition at a concentration of 0.01% to 10% by mass relative to the total mass of the composition. The fructooligosaccharide (FOS) is present in the composition at a concentration of 0.001% to 5% by mass relative to the total mass of the composition. The mannose monosaccharide is present in a concentration of 0.1% to 5% by mass relative to the total mass of the composition. The composition further comprises at least one probiotic microorganism selected from Lactobacillus acidophilus, Lactobacillus casei, and mixtures thereof. Cosmetic composition.

2. The composition according to claim 1, further comprising: silicone fatty substances, gums and waxes; non-silicone fatty substances, pastes and waxes of plant, mineral, animal and / or synthetic origin; fatty acids having 8 to 32 carbon atoms; synthetic esters and ethers; linear or branched hydrocarbons of mineral or synthetic origin; fatty alcohols having 8 to 26 carbon atoms; water; C2 to C6 monoalcohols; glycols selected from propylene glycol, butylene glycol and pentylene glycol; ketones; thickeners, emulsifiers, surfactants, gelling agents, fragrances, fillers, colorants, moisturizers, vitamins selected from vitamins A, E and B3; and at least one component selected from polymers.

3. The composition according to claim 1 or 2, wherein the oligosaccharide and / or polysaccharide is present in a concentration of 0.01% to 20% by mass relative to the total mass of the composition.

4. The composition according to any one of claims 1 to 3, wherein the probiotic microorganism is present in a concentration of 0.0001% to 10% by mass relative to the total mass of the composition.

5. A cosmetic method (excluding medical procedures) for skin care, comprising the application of a composition according to any one of claims 1 to 4 to the skin.

6. The cosmetic method according to claim 5, for maintaining and / or restoring the balance of the skin's bacterial flora.

7. The cosmetic method according to claim 6, for preventing and / or treating sensitive skin.

8. The cosmetic method according to claim 7, for preventing and / or treating the loss of aesthetic properties of the skin.

9. A cosmetic use (excluding medical procedures) for skin care, which involves topical application of at least oligosaccharides and / or polysaccharides, at least one mannose monosaccharide, and at least one probiotic microorganism, The oligosaccharide and / or polysaccharide is in the form of a mixture containing at least one gluco-oligosaccharide (GOS) and at least one fructo-oligosaccharide (FOS), The aforementioned gluco-oligosaccharide (GOS) is used in the cosmetic composition at a concentration of 0.01% to 10% by mass relative to the total mass of the composition. The fructooligosaccharide (FOS) is used in the cosmetic composition at a concentration of 0.001% to 5% by mass relative to the total mass of the composition. The mannose monosaccharide is used in the cosmetic composition at a concentration of 0.1% to 5% by mass relative to the total mass of the composition. The composition further comprises at least one probiotic microorganism selected from Lactobacillus acidophilus, Lactobacillus casei, and mixtures thereof. use.

10. The use according to claim 9, which is for maintaining and / or restoring the balance of the skin's bacterial flora.

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