Moisture-locking and repairing composition for hair and use thereof

Abstract

A moisture-locking and repairing composition for hair and the use thereof. The functional components of the composition are a yeast fermentation product filtrate or lysate, and a composite hyaluronic acid or a salt thereof, wherein the content of the yeast fermentation product filtrate or lysate is 1-90 wt%, and the content of the composite hyaluronic acid or a salt thereof is 1-20 wt%. Compared with a single component, the composition has a synergistic effect in the aspects of improving the strength and toughness of hair, preventing hair breakage, resisting frizz, etc., and has wide application prospects in the field of hair washing and hair care.

Description

A hair water-locking and repairing composition and its application Technical Field

[0001] The present invention relates to a hair moisture-locking and repairing composition and application thereof, and in particular to a hair moisture-locking and repairing composition and application thereof with the functions of improving hair toughness, preventing hair breakage and resisting frizz, and belongs to the technical field of hair products. Background Art

[0002] Today, people's demand for hair care is growing, with frequent washing, perming, dyeing, and styling. Furthermore, environmental stress can easily cause hair damage, which often leads to dry, frizzy hair, decreased hair flexibility, damaged hair, and brittle hair. Therefore, the development of active ingredients and products with hair conditioning and repairing properties has become a research hotspot. Currently, the most widely used hair repair active ingredients fall into three categories: 1) Cationic polymer conditioning agents, such as polyquaternium salts and cationic modified polysaccharides, are commonly used hair conditioning agents that improve hair flexibility and repair the hair cuticle; 2) Proteins, hydrolyzed proteins, and peptides are macromolecules that can repair damaged hair, increase its mechanical strength, and reduce breakage; 3) Small molecule moisturizing ingredients, such as panthenol, amino acids, and organic acids, have also been shown to lock in moisture and improve hair quality.

[0003] Hyaluronic acid (HA) is a macromolecular bioactive substance that can bind to large amounts of water molecules through hydrogen bonding, resulting in moisturizing and hydration. In recent years, it has been widely used in skincare products. my country's microbial fermentation-based HA production technology is a global leader. In recent years, local companies have pioneered the use of enzymatic cleavage to produce oligomeric HA, expanding the deep moisturizing, anti-inflammatory, soothing, and repairing properties of HAs of varying molecular weights. However, the application of HA, particularly oligomeric HA, in hair care technology remains limited.

[0004] Summary of the Invention

[0005] The present invention aims to provide a hair moisture-locking and repairing composition. The composition comprises a yeast fermentation product filtrate or lysate and a composite hyaluronic acid or a salt thereof. Experimental findings unexpectedly revealed that the composition has a synergistic effect compared to a single component in terms of improving hair strength, preventing hair breakage, and combating frizz. The composition has broad application prospects in the field of hair shampooing and hair care.

[0006] The present invention provides a hair moisture-locking and repairing composition, the effective ingredients of which are yeast fermentation product filtrate or lysate and composite hyaluronic acid or its salt, the content of yeast fermentation product filtrate or lysate is 1-90wt%, the content of composite hyaluronic acid or its salt is 1-20wt%, and the total content of the two is less than or equal to 100wt%.

[0007] Furthermore, in the above-mentioned hair moisture-locking and repairing composition, the content of the yeast fermentation product filtrate or lysate is preferably 5 to 50 wt %, more preferably 5 to 30 wt %.

[0008] Furthermore, in the above-mentioned hair moisture-locking and repairing composition, the content of the composite hyaluronic acid or its salt is 1 to 15 wt %, more preferably 1 to 10 wt %.

[0009] For example, the content of the yeast fermentation product filtrate or lysate can be 1 wt%, 5 wt%, 10 wt%, 15 wt%, 20 wt%, 25 wt%, 30 wt%, 35 wt%, 40 wt%, 45 wt%, 50 wt%, 55 wt%, 60 wt%, 65 wt%, 70 wt%, 75 wt%, 80 wt%, 85 wt%, 90 wt%, etc. The content of the composite hyaluronic acid or its salt can be 1 wt%, 2 wt%, 3 wt%, 4 wt%, 5 wt%, 6 wt%, 7 wt%, 8 wt%, 9 wt%, 10 wt%, 11 wt%, 12 wt%, 13 wt%, 14 wt%, 15 wt%, 16 wt%, 17 wt%, 18 wt%, 19 wt%, 20 wt%, etc.

[0010] Furthermore, the ratio of the yeast fermentation product filtrate or lysate to the composite hyaluronic acid or its salt is 1:4 to 9:1, preferably 1:3 to 2:1.

[0011] For example, the ratio of the yeast fermentation product filtrate or lysate to the complex hyaluronic acid or its salt can be 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, etc.

[0012] Furthermore, the hair moisture-locking and repairing composition may contain water as a solvent in addition to the effective ingredients, and the water content may be up to 100 wt%.

[0013] Furthermore, the yeast fermentation product filtrate or lysate described in the present invention refers to the fermentation filtrate or lysate obtained by yeast fermenting rice, beans, or wheat. For example, the fermentation filtrate is the fermentation product obtained by yeast propagation and / or fermentation in a culture medium containing rice, beans, or wheat. The fermentation product filtrate is obtained by filtering the fermentation product, and the fermentation product lysate is the substance in the cells of the fermentation product obtained by disrupting the cells in the fermentation product. In addition to containing rice, beans, or wheat, the culture medium may also contain one or more of the following fermentation-required ingredients: a carbon source (sugar), a nitrogen source (yeast powder, peptone, etc.), and inorganic salts, depending on the fermentation requirements.

[0014] Preferably, the yeast fermentation product filtrate or lysate is a fermentation filtrate or lysate obtained by fermenting rice, especially brown rice (whole grain rice), with yeast, which can be referred to as yeast / rice fermentation product filtrate or yeast / rice fermentation lysate. The yeast / rice fermentation product filtrate is prepared by the following method: brown rice is germinated and then crushed into brown rice flour, which is then prepared into rice milk, which is enzymatically hydrolyzed and then added with yeast for fermentation, and the fermentation liquid is removed from impurities and sterilized to obtain the obtained product.

[0015] Furthermore, the composite hyaluronic acid or its salt is a composition of oligomeric hyaluronic acid or its salt and low molecular weight hyaluronic acid or its salt, wherein the molecular weight of the oligomeric hyaluronic acid or its salt is less than or equal to 8 kDa, preferably less than or equal to 5 kDa, and the molecular weight of the low molecular weight hyaluronic acid or its salt is 20 kDa-80 kDa, preferably 30 kDa-50 kDa.

[0016] Furthermore, the mass ratio of the oligomeric hyaluronic acid or its salt to the low molecular weight hyaluronic acid or its salt is 1:5-5:1, preferably 1:4-3:2.

[0017] For example, the mass ratio of the oligomeric hyaluronic acid or its salt to the low molecular weight hyaluronic acid or its salt can be 1:5, 1:4.5, 1:4, 1:3.5, 1:3, 1:2.5, 1:2, 1:1.5, 1:1, 1.5:1, 2:1, 3:1, 4:1, 5:1, etc.

[0018] Furthermore, salts of oligomeric or low molecular weight hyaluronic acid include sodium salt, potassium salt, calcium salt, zinc salt, magnesium salt and the like.

[0019] The hair moisture-locking and repairing composition of the present invention exhibits unexpected synergistic effects in improving hair strength, preventing hair breakage, and combating frizz, and has promising application prospects in hair products. These products can be either hair care products or hair cleansing products, and can be either rinse-off or leave-on.

[0020] The present invention also provides a hair product, the effective ingredients of which include the hair moisture-locking and repairing composition of the present invention.

[0021] Furthermore, the above-mentioned hair products, in addition to the hair moisture-locking and repairing composition of the present invention, may also include other functional ingredients, such as other ingredients with moisturizing, hydrating, moisture-locking, repairing, cleaning, anti-dandruff, and anti-hair loss effects, such as peptides, panthenol, amino acids, etc.

[0022] Furthermore, the content of the hair moisture-locking and repairing composition in the hair product is 0.5-10 wt %, preferably 1-5 wt %, and more preferably 2-3 wt %.

[0023] For example, the content of the hair moisture-locking and repairing composition in the hair product may be 0.5wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, 6.5wt%, 7wt%, 7.5wt%, 8wt%, 8.5wt%, 9wt%, 9.5wt%, 10wt%, etc.

[0024] Furthermore, the hair product can be a hair care product or a hair cleansing product. The hair product can be either a rinse-off type or a leave-on type.

[0025] Furthermore, the hair products may be various types such as shampoo, conditioner, hair mask, spray, etc.

[0026] Furthermore, the hair product may also include auxiliary ingredients necessary for preparing different dosage forms. Each auxiliary ingredient may be selected from the existing technology, and the preparation method of the dosage form may also be carried out according to the existing technology.

[0027] The present invention compounds yeast fermentation product filtrate or lysate, oligomeric hyaluronic acid or its salt, low molecular weight hyaluronic acid or its salt. Yeast fermentation product filtrate or lysate contains rich active substances such as amino acids, polypeptides, minerals, etc., which can penetrate into the hair core and play a role in repairing damaged chemical bonds and thus repairing keratin. Hyaluronic acid selects different molecular weights for compounding, which can achieve its effective penetration and distribution in hair, achieve the effect of three-dimensional repair and water lock, strengthen the hydrogen bonding effect inside the hair, and realize keratin structure consolidation, thereby effectively improving hair toughness, repairing and / or protecting the problem of impaired mechanical properties of hair brought about by various damage pressures (including bleaching, perming and dyeing hair, pollution exposure, ultraviolet irradiation, multiple washings, high temperature, etc.); In addition, the composition also has the effect of lubricating hair surface and resisting curling and frizz, and improves the use performance of hair care products and hair cleaning products. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a fluorescence micrograph of fluorescein-labeled sodium hyaluronate penetrating into hair, A is 30-50 kDa sodium hyaluronate, and B is 1460 kDa high-molecular sodium hyaluronate.

[0029] FIG2 shows the strength-repairing effect of the composition of the present invention on damaged hair and healthy hair in various conditions.

[0030] FIG3 shows the anti-frizz effect of the composition in Example 1 of the present invention on hair. DETAILED DESCRIPTION

[0031] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below with reference to specific embodiments and drawings.

[0032] In the following examples, the yeast / rice fermentation product filtrate used is INCI named SACCHAROMYCES / RICE FERMENT FILTRATE, and the raw materials used are commercially available products with the trade names: Brown Rice Ferment Filtrate (Bioyouth TM -Brice). The lactobacillus / rye flour fermentation product used was a commercially available product. The hyaluronic acid used was sourced from Bloomage Biotechnology Co., Ltd.

[0033] Test Example 1 Tensile Strength Test

[0034] Weigh the raw materials according to the formula in Table 1 below, add the raw materials into water, and mix them evenly to obtain a hair moisture-locking and repairing composition.

[0035] Table 1

[0036] The tensile strength test of the hair moisture-locking and repairing composition prepared above was performed using the following method:

[0037] First, the compositions prepared in each Example and Comparative Example were diluted with deionized water. The aqueous solutions of the compositions except those in Example 2 were diluted with water so that the active ingredient content in each composition solution was 2.5 wt %. The prepared solutions were placed in spray bottles or pump bottles and used as test samples.

[0038] #For Comparative Example 5, due to the high viscosity of 1460KDa sodium hyaluronate, it cannot be prepared as a 10% solution, so it is prepared as a 2% solution, and the addition amount of yeast / rice fermentation product filtrate is also adjusted to 2%.

[0039] Thirteen heavily damaged hair tresses (multiple bleached hair tresses, 15 cm long, 1 g in weight) were selected and pre-shampooed with 10% SDS. The hair was then air-dried at a constant temperature and humidity of (25±2)°C and (50±5)%. After the hair tresses were completely dry, twelve of the tresses were sprayed with an equal amount (0.3 g) of the test sample from each example and comparative example, and one tress was sprayed with an equal amount (0.3 g) of water, mimicking the use of a hair spray. After air-drying at a constant temperature and humidity, this procedure was repeated ten times. After the final application and air-drying, all tresses were rinsed with deionized water to remove any residual sample from the surface of the tresses, and then air-dried at a constant temperature and humidity. The hair diameters were measured using an SN-1200W high-definition camera, averaging the diameters at three locations in the middle of the hair. Thirty hair strands with diameters within 10 μm from each of the thirteen hair bundles were tested using a fiber strength tester. The tensile strength of the control group and each sample group was calculated and compared using the following formula: σ = Fb / So

[0040] Where σ is the tensile strength, Fb is the maximum force at break, and So is the original cross-sectional area of ​​the specimen. Furthermore, the average elastic modulus of the hair is calculated from the elastic modulus area of ​​the tensile curve.

[0041] The results of the hair tensile strength test are shown in Table 2 below:

[0042] Table 2

[0043] As can be seen from the results in the above table, the tensile strength of Examples 1-7 is higher than that of the blank control, indicating that the combination of yeast / rice fermentation product filtrate and sodium hyaluronate is indeed beneficial for improving the strength of hair.

[0044] The tensile strength of Example 1 was significantly higher than that of Comparative Examples 1 and 2, as well as the blank control. This indicates that the combination of yeast / rice fermentation filtrate and two-molecular-weight sodium hyaluronate improves hair strength more effectively than the combination of yeast / rice fermentation filtrate and sodium hyaluronate alone, demonstrating the necessity of combining two-molecular-weight sodium hyaluronate. The tensile strength results of Examples 1-7 demonstrate that the closer the ratio of yeast / rice fermentation filtrate to two-molecular-weight sodium hyaluronate is to 1:1, the more pronounced the improvement in hair strength.

[0045] Furthermore, the tensile strength of Example 1 was higher than that of Comparative Examples 3 and 4, demonstrating that the yeast / rice fermentation product filtrate and the two sodium hyaluronates in the composition exhibit synergistic effects in improving hair strength, while substitution of other types or varieties of fermentation product filtrate lacked a significant synergistic effect. The tensile strengths produced by Example 1 and Comparative Examples 1 and 2 were significantly higher than those of Comparative Example 5, demonstrating that the choice of hyaluronic acid molecular weight significantly impacts hair strength.

[0046] As shown in Figure 1, the results of the fluorescein-labeled sodium hyaluronate penetration experiment in hair show that the 1460kDa high molecular weight sodium hyaluronate has no permeability, while the 30-50kDa sodium hyaluronate can penetrate into the hair and even the hair core, thus achieving a repairing effect. The specific experimental steps are as follows:

[0047] Place 0.2g of sodium hyaluronate in a stoppered test tube and add 2mL of 0.05mol / L NaOH aqueous solution. Stopper and seal the tube, then vortex to dissolve the sodium hyaluronate. Accurately add 0.04g of FITC, seal the tube, mix thoroughly using a vortex mixer, and place in a 95°C water bath. Incubate for 45 minutes, then remove from the tube. Cool to room temperature and add 18mL of sodium chloride-saturated anhydrous ethanol. Centrifuge and discard the supernatant. The resulting precipitate is the crude FITC-labeled sodium hyaluronate. Add 20mL of sodium chloride-saturated anhydrous ethanol to the precipitate, vortex to evenly disperse the labeled sodium hyaluronate precipitate in the sodium chloride-saturated anhydrous ethanol. Centrifuge and discard the supernatant. This is the first alcohol wash. Repeat this alcohol wash six times, then freeze-dry the precipitate to obtain the sodium hyaluronate-FITC fluorescent marker. The experiment involved carefully measuring 0.05g of sodium hyaluronate-FITC fluorescent marker into a stoppered test tube. 10mL of deionized water was added, sealed, and vortexed to dissolve. Twenty randomly selected hair strands were immersed in the fluorescently labeled sodium hyaluronate solution. After soaking for 4 hours, the strands were removed and thoroughly rinsed to remove any residual sodium hyaluronate. Cross-sections of the hair strands were then obtained using cryosectioning and observed using a fluorescence microscope. As can be seen in the figure, the 1460kDa fluorescently labeled high-molecular-weight sodium hyaluronate (Figure 1, right) showed minimal penetration into the hair, while the 30-50kDa fluorescently labeled sodium hyaluronate (Figure 1, left) exhibited significant fluorescence throughout the hair strands, even within the hair core.

[0048] Further analysis of the tensile curve characteristics obtained in each experiment shows that the elastic modulus and elongation at break of hair are different when tested with different test samples. The elastic modulus and elongation at break of hair of some test samples are shown in Table 3 below:

[0049] Table 3

[0050] Note: * indicates that the statistical data of this group are significantly different from those of the blank control group (P<0.05).

[0051] The above results show that the effects of yeast / rice fermentation product filtrate on the mechanical properties of hair vary when combined with different hyaluronic acids. When yeast / rice fermentation product filtrate is combined with oligomeric sodium hyaluronate (Comparative Example 1), the hair's elongation at break is significantly improved, while the hair's elastic modulus is slightly affected. When yeast / rice fermentation product filtrate is combined with low-molecular sodium hyaluronate (Comparative Example 2), the hair's elastic modulus is significantly improved, while the hair's elongation at break is slightly affected. When yeast / rice fermentation product filtrate is compounded with two sodium hyaluronates, both the hair's elastic modulus and elongation at break are significantly improved (Example 1). Therefore, it is necessary to compound the two types of sodium hyaluronate in the composition. The principle is that low molecular weight sodium hyaluronate and oligomeric sodium hyaluronate have complementary effects in enhancing the strength of hair: low molecular weight sodium hyaluronate is more effective in improving the elastic modulus of hair, while oligomeric sodium hyaluronate is more effective in improving the elongation at break of hair. This may be because low molecular weight sodium hyaluronate has a strong water-locking effect and dominates the strengthening of the hydrogen bond network; while oligomeric sodium hyaluronate has higher activity and permeability, and thus interacts stronger with keratin.

[0052] Test Example 2 Antioxidant Activity Test

[0053] The yeast / rice fermentation product filtrate is rich in active ingredients such as peptides, amino acids, phytic acid, ferulic acid, lactic acid, phytamides, and sterols. These small-molecule active ingredients not only enhance the composition's moisture retention and hair strength through high permeability, but also possess antioxidant and free radical scavenging properties. Given that hair generates oxidative free radicals under UV irradiation, further damaging the hair structure and weakening its strength, the yeast / rice fermentation product filtrate in the composition also provides protection against UV damage.

[0054] To verify this antioxidant property, solutions of the hair moisturizing and repairing compositions from Example 1, Comparative Example 3, and Comparative Example 4 were diluted with deionized water to a 2.5% wt. concentration, each serving as test samples. The hair treatment method included selecting healthy hair tresses, washing them with 10% SDS, and storing them under constant temperature and humidity conditions of (25±2)°C and (50±5)%. Each tress was treated according to the method listed in Table 4, with UV irradiation performed in a xenon lamp simulation chamber. After 24 hours of irradiation, an equal amount of the sample was sprayed onto the tresses. The treated hair was then tested for tensile strength according to the method in Test Example 1.

[0055] Determination of DPPH scavenging rate: 2 mL of a 2 mmol / L DPPH solution was used as a blank control. 2 mL of anhydrous ethanol was used instead of the DPPH solution in the control group. The sample group consisted of 2 mL of anhydrous ethanol and 2 mL of the sample from Example 1, Comparative Example 3, and Comparative Example 4 diluted to a 2.5 wt% concentration. The mixture was thoroughly shaken and incubated in the dark for 30 minutes. The absorbance (D) was measured at 517 nm. This experiment was repeated three times. The scavenging rate was calculated and the antioxidant activity of each sample was analyzed. Scavenging rate (%) = [1 - (D - D) / D × 100%.

[0056] The experimental results are shown in Table 4 below.

[0057] Table 4

[0058] As can be seen from the above data, when healthy hair is not treated with any product, the strength decreases by 19.0% after ultraviolet irradiation. However, after Example 1 is treated with the composition, the degree of decrease in strength after ultraviolet irradiation is significantly reduced, which is significantly less than that of Comparative Examples 3 and 4, indicating that the composition of Example 1 has ultraviolet protection properties for hair, indicating that the combination of the yeast / rice fermentation product filtrate of the present application and the compounded hyaluronic acid has synergistic antioxidant activity.

[0059] Test Example 3 Combing Performance Test

[0060] The experiment was conducted at a temperature of (25±2)°C and a humidity of (50±5)%. A 40cm long, 25g human hair piece was thoroughly moistened with 40°C warm water. Using a dropper, 5mL of a 10% SDS aqueous solution was evenly applied to both sides of the piece and rubbed to create a lather. Avoid folding or twisting the hair together to prevent tangles. Rinse off the lather with warm water. Repeat this process twice before allowing the hair to air dry in a constant temperature and humidity environment.

[0061] The hair moisture-locking and repairing compositions of Example 1 and Comparative Examples 1-3 were diluted with deionized water until the hair moisture-locking and repairing compositions were 2.5 wt % each, and used as test samples.

[0062] 1.5g of each test sample prepared in each example and comparative example was accurately measured and evenly sprayed onto a hair tress. The hair tress was then briefly combed using a comb, with water serving as a blank control. After the hair tresses were air-dried in a constant temperature and humidity environment, the dry combing work of each tress was measured using a combing instrument. Two hair pieces of the same specifications were used for the test, and the test was repeated seven times. Data processing yielded a combability curve, which was then integrated to determine the combing work. Comparing the combing work before and after use revealed the sample's ability to improve the combability of the hair tress.

[0063] The experimental results are shown in Table 5 below:

[0064] Table 5 Dry combing work of hair (unit: *10cN*mm)

[0065] As shown in the results of Comparative Examples 1, 2, and the blank control in the table above, the combination of single hyaluronic acid and yeast / rice fermentation product filtrate can reduce the combing work of hair. Comparing the results of Example 1 with Comparative Examples 1 and 2, it can be seen that the combination of compounded hyaluronic acid and yeast / rice fermentation filtrate is more effective in reducing the combing work of hair than the combination of single molecular weight hyaluronic acid and yeast / rice fermentation filtrate. Furthermore, the results of Example 1 and Comparative Example 3 show that the combination of compounded hyaluronic acid and yeast / rice fermentation filtrate achieves a better combing work effect than the combination of compounded hyaluronic acid and other fermentation filtrates. This demonstrates that the composition has the technical effect of optimizing the combing properties of hair bundles. This is because the composition contains active ingredients of different molecular weights, which can also form a dense and well-developed lubricating film on the hair surface, lubricating the hair strands. Improving combing properties is very important for preventing hair breakage, as hair that is difficult to comb and easily tangled is a major cause of hair breakage during combing. Therefore, the composition of the present invention can not only improve the toughness of single hair fibers, but also improve the combing properties of hair bundles, thereby achieving a comprehensive technical effect of preventing hair breakage.

[0066] Test Example 4: Tensile Strength Test of Hair of Different Conditions

[0067] In the aforementioned experiments, the damaged hair used was damaged hair caused by multiple bleaching. In daily life, hair faces various different pressures, such as hair dyeing, chemical perming, exposure to pollutants, ultraviolet radiation, multiple washing, thermal straightening, etc.

[0068] To test the effect of the composition of Example 1 on the strength of various types of damaged hair, a solution of the hair-moisturizing and repairing composition of Example 1 was diluted with water to create a test sample with a concentration of 2.5 wt%. Following the method of Experimental Example 1, this test sample was applied to various types of damaged hair, and the tensile strength of the hair before and after application was measured. Pure water was used as a blank control. The experimental results are shown in Figure 2, demonstrating that the composition of the present invention is suitable for repairing the strength of various types of damaged hair while also enhancing the strength of healthy hair.

[0069] Test Example 5 Anti-frizz performance

[0070] The hair moisture-locking and repairing composition of Example 1 was diluted with water to prepare a test sample with a concentration of 2.5 wt%.

[0071] Anti-frizz test: Six hair tresses (3 healthy tresses and 3 damaged tresses, each 15 cm long and 1 g in weight) were pre-shampooed with 10% SDS and air-dried at a constant temperature and humidity of (25±2)°C and (50±5)%. Once completely dry, each tress was sprayed with 0.3 g of deionized water and the test sample. After air-drying under constant temperature and humidity, the tresses were placed in an environment with a humidity of approximately 80% (simulating a rainy day). Photos were taken at 0 and 4 hours. The degree of frizz and volume of the tresses in the high-humidity environment were compared to assess the product's anti-frizz performance.

[0072] The experimental results are shown in Figure 3. Figure 3a shows a healthy hair tress before treatment in a high-humidity environment (for before-and-after comparison), Figure 3b shows an image of a healthy hair tress treated with the test sample after standing at 80% high humidity for 4 hours, and Figure 3c shows an image of a healthy hair tress treated with deionized water after standing at 80% high humidity for 4 hours. Figure 3e shows a damaged hair tress before treatment in a high-humidity environment (for before-and-after comparison), Figure 3d shows an image of a damaged hair tress treated with the test sample after standing at 80% high humidity for 4 hours, and Figure 3f shows an image of a damaged hair tress treated with deionized water after standing at 80% high humidity for 4 hours. As shown in Figure 3, the hair tress untreated with the test sample significantly increases in volume in a humid environment, with the hair tress, especially the ends, becoming frizzy and tangled. However, the hair tress treated with the test sample shows minimal volume change in a high-humidity environment. Therefore, the composition of the present invention also has anti-frizz and anti-curling properties, preventing hair from becoming unmanageable in high-humidity conditions.

[0073] Test Example 6: Hair Moisturizing Efficacy Test

[0074] Hair contains both free and bound water, with free water having a greater impact on hair properties. Excessive free water often disrupts hydrogen bonds within the hair and reduces keratin crystallinity, leading to a decrease in hair strength. The composition of the present invention combines sodium hyaluronate of varying molecular weights with a yeast / rice fermentation product filtrate. The water-locking component has a molecular weight ranging from tens of thousands to hundreds of thousands and contains a rich array of moisturizing groups (carboxyl, amino, and hydroxyl groups). This creates a three-dimensional water-locking effect, strengthens hydrogen bonding within the hair, and consolidates the keratin structure, ultimately enhancing hair strength.

[0075] The hair moisture retention efficacy test method is as follows: The experiment was conducted at a temperature of (25±2)°C and a humidity of 50±5%. Six 40cm long, 25g human hair swatches were pre-shampooed with 10% SDS and then air-dried in a constant temperature and humidity environment for 24 hours. A certain amount of each swatch was first placed in a moisture analyzer at 65°C for 20 minutes to measure the water loss rate at 65°C (reflecting the free water content in the hair, excluding structural water). Then, 1g of deionized water and 1g of a 0.5% diluted hair moisture-repairing composition were precisely measured and evenly sprayed onto the swatches. The swatches were then air-dried at a constant temperature and humidity for 12 hours, then rinsed to remove any residual sample from the surface. This procedure was repeated ten times, and a certain amount of each swatch was then placed in a moisture analyzer to measure the water loss rate at 65°C. The thermal weight loss of the hair during this process was recorded to determine the free water content in the hair. The experimental results are shown in Table 6 below:

[0076] Table 6

[0077] As can be seen from the results in the table above, Example 1 significantly reduces the free water content in hair. This demonstrates that the composition of the present invention has the best hair moisture retention, and this moisture retention is a key factor in improving hair strength and producing the anti-frizz and frizz-fighting effects.

[0078] Test Example 7 Screening of Hair Moisturizing and Repairing Composition Content

[0079] The hair moisturizing and repairing composition of Example 1 was diluted with deionized water to obtain the active ingredient content of the hair moisturizing and repairing composition as shown in Table 7. The resulting aqueous solutions containing different weight percentages of the hair moisturizing and repairing composition were tested for hair tensile strength using the method described in Test Example 1. The results are shown in Table 7.

[0080] Table 7

[0081] The above results indicate that, in actual use, the composition does not exhibit a significant effect on enhancing hair strength at a concentration of 0.1%, whereas a significant effect is exhibited at concentrations between 0.5% and 10%. Therefore, the composition is preferably used in hair products at a concentration of 0.5% to 10%. Furthermore, the composition is more preferably used in hair products at a concentration of 1% to 5%, and even more preferably, at a concentration of 2% to 3%.

[0082] Test Example 8 Screening of the Compound Ratio of Oligomeric Sodium Hyaluronate and Low Molecular Sodium Hyaluronate

[0083] According to the method of Example 1, yeast / rice fermentation product filtrate, oligomeric sodium hyaluronate, low-molecular sodium hyaluronate, and water were mixed to prepare a hair moisturizing and repairing composition solution having a yeast / rice fermentation product filtrate content of 10 wt% and a total content of oligomeric sodium hyaluronate and low-molecular sodium hyaluronate of 10 wt%. The mass ratio of oligomeric sodium hyaluronate to low-molecular sodium hyaluronate was varied to obtain multiple sets of hair moisturizing and repairing composition solutions as shown in Table 8 below. Each set of hair moisturizing and repairing composition solutions was diluted with water to obtain test samples containing 2.5 wt% of the hair moisturizing and repairing composition. The test samples were tested for hair tensile strength according to the method of Test Example 1. The experimental results are shown in Table 8 below:

[0084] Table 8

[0085] The above experimental results demonstrate that a ratio of oligomeric sodium hyaluronate to low-molecular sodium hyaluronate within the range of 1:5 to 5:1 effectively enhances hair strength, demonstrating a significant synergistic effect. Specifically, a ratio of 3:2 to 1:4 significantly enhances hair strength by over 20%. Therefore, the optimal mass ratio of oligomeric sodium hyaluronate to low-molecular sodium hyaluronate is 3:2 to 1:4.

Claims

1. A hair moisture-locking and repairing composition, characterized in that: The effective ingredients include yeast fermentation product filtrate or lysate and composite hyaluronic acid or its salt, the content of yeast fermentation product filtrate or lysate is 1-90wt%, and the content of composite hyaluronic acid or its salt is 1-20wt%.

2. The hair moisture-locking and repairing composition according to claim 1, characterized in that: The content of the yeast fermentation product filtrate or lysate is 5-50wt%, and the content of the composite hyaluronic acid or its salt is 1-15wt%; preferably, the content of the yeast fermentation product filtrate or lysate is 5-30wt%, and the content of the composite hyaluronic acid or its salt is 1-10wt%.

3. The hair moisture-locking and repairing composition according to claim 1 or 2, characterized in that: The ratio of the yeast fermentation product filtrate or lysate to the composite hyaluronic acid or its salt is 1:4 to 9:1, preferably 1:3 to 2:

1.

4. The hair moisture-locking and repairing composition according to any one of claims 1 to 3, characterized in that: The composite hyaluronic acid or its salt is a composition of oligomeric hyaluronic acid or its salt and low molecular weight hyaluronic acid or its salt. The mass ratio of the oligomeric hyaluronic acid or its salt to the low molecular weight hyaluronic acid or its salt is 1:5 to 5:1, preferably 1:4 to 3:

2.

5. The hair moisture-locking and repairing composition according to claim 4, characterized in that: The molecular weight of the oligomeric hyaluronic acid or its salt is less than or equal to 8 kDa, preferably less than or equal to 5 kDa, and the molecular weight of the low molecular weight hyaluronic acid or its salt is 20 kDa-80 kDa, preferably 30 kDa-50 kDa.

6. The hair moisture-locking and repairing composition according to claim 4, characterized in that: The salts of oligomeric hyaluronic acid and low molecular weight hyaluronic acid are both selected from sodium salt, potassium salt, calcium salt, zinc salt or magnesium salt thereof.

7. The hair moisture-locking and repairing composition according to any one of claims 1 to 3, characterized in that: The yeast fermentation product filtrate or lysate refers to the fermentation filtrate or lysate obtained by fermenting rice, beans or wheat and other plants with yeast; preferably, the yeast fermentation product filtrate or lysate refers to the fermentation filtrate or lysate obtained by fermenting rice with yeast.

8. The hair moisture-locking and repairing composition according to any one of claims 1 to 7, characterized in that: Also includes water, which makes up the balance.

9. Use of the hair moisture-locking and repairing composition according to any one of claims 1 to 8 in a hair product; preferably, the hair product is a rinse-off or leave-on hair care product, or a rinse-off or leave-on hair cleansing product.

10. A hair product, characterized in that: The effective ingredients include the hair moisture-locking and repairing composition according to any one of claims 1 to 8.

11. The hair product according to claim 10, characterized in that: The content of the hair moisture-locking and repairing composition in the hair product is 0.5-10%, preferably 1-5%, more preferably 2-3%; preferably, the hair product is a rinse-off and / or leave-on hair care product or a rinse-off and / or leave-on hair cleaning product.

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