Composition for improving soil environment comprising hypnum plumaeforme wilson or microorganisms derived therefrom, and method for improving soil environment using same

Abstract

The present invention relates to a composition for improving a soil environment comprising Hypnum Plumaeforme Wilson or microorganisms derived therefrom, and a method for improving a soil environment using same. A soil environment for plant growth can be created by treating soil with the composition according to the present invention to increase the organic matter, nitrogen content, cation exchange capacity, etc. of the soil.

Description

Composition for improving soil environment containing hairy feather moss or microorganisms derived therefrom, and method for improving soil environment using the same

[0001] The present invention relates to a composition for improving a soil environment comprising Hypnum plumaeforme or microorganisms derived therefrom, and a method for improving a soil environment using the same, and more specifically, to a composition capable of creating an environment for plant growth by increasing organic matter, total nitrogen content, and cation exchange capacity of the soil by applying the composition to the soil.

[0002] As bryophytes, mosses were the first plants to adapt to life on land, and possess exceptional environmental adaptability and survival capabilities. This resilience allows mosses to be found in every region of the globe, from tropical rainforests to tundra, excluding deserts and oceans. They are also among the first species to emerge in areas devastated by natural disasters like forest fires and droughts.

[0003] Meanwhile, soils that do not have the conditions for plant growth, such as depleted or polluted soil, can be stabilized by moss and biodiversity can increase in the future. However, because it takes a considerable amount of time for moss to grow sufficiently, improving the soil environment using moss may be inefficient in terms of time and physical aspects.

[0004] Therefore, if microorganisms with soil growth improvement potential are isolated from existing moss with confirmed soil environment improvement ability, it is expected that the efficiency of soil environment improvement can be increased by treating the microorganisms as a substitute for moss or treating them in combination with moss.

[0005] Accordingly, the inventors of the present invention confirmed that when hairy feather moss or microorganisms isolated therefrom are treated and cultured in soil, it is possible to improve the soil environment by increasing the organic matter, nitrogen content, and cation exchange capacity in the soil.

[0006] Accordingly, the purpose of the present invention is to provide a composition for improving soil environment containing Hypnum plumaeforme.

[0007] Another object of the present invention is to provide a microorganism isolated from a hairy feather moss.

[0008] Another object of the present invention is to provide a composition for improving soil environment containing microorganisms derived from hairy moss.

[0009] Another object of the present invention is to provide a method for improving the soil environment through a composition for improving the soil environment.

[0010] The present invention relates to a composition for improving a soil environment including a hairy moss or a microorganism derived therefrom, and a method for improving a soil environment using the same. The composition of the present invention enables improvement of the soil environment by increasing the organic matter or total nitrogen content and cation exchange capacity in the soil.

[0011] Hereinafter, the present invention will be described in more detail.

[0012] One aspect of the present invention relates to a composition for improving soil environment, comprising Hypnum plumaeforme, a pulverized product thereof, or a culture thereof.

[0013] The term "Hypnum moss" in this specification refers to a plant species belonging to the genus Hypnum of the Hypnaceae family among bryophytes, which is distributed throughout Asia including Korea, China, and Japan, as well as Russia and Hawaii, and is a perennial moss species that grows in a mat-like shape on rocks or the ground in sunny mountains and fields.

[0014] The term "crushed material" in this specification means a state in which an object is broken into pieces or particles due to an external force applied to the object, and the crushed material may have an average particle size of 0.01 to 50 mm, and more specifically, may have an average particle size of 0.5 to 10 mm, but is not limited thereto.

[0015] When producing a powdered product of the hairy moss according to the present invention, not only hairy moss that has not undergone a separate treatment process, but also frozen hairy moss, dried hairy moss, freeze-dried hairy moss, or hairy moss that has undergone other preservation treatments can be used.

[0016] The term "culture" in this specification means a material obtained by growing or propagating a subject for a predetermined period of time in an environment appropriate to the growth environment of the subject, and obtaining part or all of the subject upon completion of growth. The culture may further contain other organisms or biological or chemical impurities other than the subject to be grown.

[0017] The term "soil environment" in this specification refers to all physical, chemical or biological properties of the soil, and more specifically, may refer to, but is not limited to, soil organic matter content, total nitrogen content in the soil, ion content in the soil, phosphate content in the soil, characteristics of soil microbial communities, content of plant-useful microorganisms in the soil, cation exchange capacity of the soil and soil pH, etc. that affect soil-based plant growth.

[0018] The term "soil environment improvement" in this specification means changing the soil environment so as to ensure the stability, diversity, and sustainability of the soil-based ecosystem, and more specifically, it can mean changing the soil environment so as to be more suitable for the growth of plant groups that play a producer role in the soil ecosystem, thereby ensuring the stability and diversity of the soil ecosystem. It is thought that the composition comprising the hairy feather moss or microorganisms derived from the hairy feather moss according to the present invention can increase the organic matter content, nitrogen content, and cation exchange capacity in the soil, and thus can be utilized as a preparation for improving the plant growth environment for polluted or depleted soil (Figs. 6 to 11).

[0019] The composition according to the present invention may further include additives generally used for soil management, for example, organic additives such as organic acids, peat, or crushed and fermented products of organisms, inorganic additives including minerals such as sand, clay, and zeolite, soil microbial agents, soil activators, or molding agents such as thickeners for molding soil particles, but are not limited thereto.

[0020] Another aspect of the present invention relates to a microorganism isolated from a hairy moss.

[0021] The term "microorganism" in this specification collectively refers to organisms having a size that is difficult to identify with the naked eye, for example, a size of less than 0.1 mm. Microorganisms such as bacteria, fungi, algae, flagellates, ciliates, spores, amoebas, slime molds, or oomycetes can be isolated from the hairy moss according to the present invention, and for example, the microorganism isolated from the hairy moss can be bacteria.

[0022] The microorganism according to the present invention may inhabit the surface of the root or frond of the hairy feather moss, or inside the tissue of the hairy feather moss.

[0023] In one embodiment of the present invention, the microorganism may be Priestia aryabhattai.

[0024] In one embodiment of the present invention, Priestia aryabhattai may be a strain deposited with the Biological Resource Center of the Korea Research Institute of Bioscience and Biotechnology under the deposit number KCTC19097P and named as “Priestia aryabhattaiCOFN001.”

[0025] Another aspect of the present invention relates to a composition for improving soil environment, comprising a microorganism derived from hairy moss or a culture thereof.

[0026] In one embodiment of the present invention, the microorganism may be Priestia ariabatae.

[0027] Another aspect of the present invention relates to a method for improving a soil environment, comprising the following steps: a treatment step of treating soil with a composition for improving a soil environment, the composition comprising at least one selected from the group consisting of hairy moss, a pulverized product of hairy moss, a culture of hairy moss, a microorganism derived from hairy moss, and a culture of a microorganism derived from hairy moss.

[0028] In one embodiment of the present invention, the treatment step may be a complex treatment of a moss composition comprising at least one selected from the group consisting of a hairy moss, a pulverized hairy moss, and a culture of a hairy moss, and a microbial composition comprising at least one selected from the group consisting of a hairy moss-derived microorganism, and a culture of a hairy moss-derived microorganism.

[0029] When the moss composition and the microbial composition according to the present invention are combined, the moss composition and the microbial composition may be treated simultaneously or sequentially at predetermined time intervals. For example, the soil may be treated with the moss composition 0, 1, 3, 5, 7, 14, 21, or 28 days after the microbial composition is treated. Conversely, the soil may be treated with the moss composition first and then the microbial composition may be treated.

[0030] In one embodiment of the present invention, the method may further include a soil culturing step of cultivating soil treated with a composition for improving soil environment for a predetermined period of time.

[0031] When cultivating soil treated with a composition for improving soil environment, a synergistic improvement effect on growth conditions can be obtained through the growth of microorganisms sprayed on the soil and the interaction between microorganisms and moss.

[0032] The present invention relates to a composition for improving a soil environment including a hairy moss or a microorganism derived therefrom, and a method for improving a soil environment using the same. When the composition according to the present invention is applied to soil, the organic matter or nitrogen content and cation exchange capacity in the soil increase, and therefore, the soil environment of devastated or contaminated soil can be improved through the composition of the present invention.

[0033] FIG. 1 is a diagram showing a phylogenetic tree according to the strain identification results of Sample 1 and Sample 2 among nine types of microorganisms selected from Feathery Moss according to one embodiment of the present invention.

[0034] FIG. 2 is a diagram showing a phylogenetic tree according to the strain identification results of samples 3 and 5 to 7 among nine types of microorganisms selected from hairy moss according to one embodiment of the present invention.

[0035] FIG. 3 is a diagram showing a phylogenetic tree according to the strain identification results of sample 4 among 9 types of microorganisms selected from hairy feather moss according to one embodiment of the present invention.

[0036] FIG. 4 is a diagram showing a phylogenetic tree according to the strain identification results of Sample 8 and Sample 9 among nine types of microorganisms selected from Feathery Moss according to one embodiment of the present invention.

[0037] Figure 5 is a photographic material showing a schematic process of evaluating the soil environment improvement ability of hairy moss or hairy moss-derived microorganisms.

[0038] Figure 6 is a graph comparing the organic matter (%) content of reclaimed land soil treated with feather moss according to one embodiment of the present invention with that of the untreated group.

[0039] Figure 7 is a graph comparing the total nitrogen content N (%) when the hairy feather moss according to one embodiment of the present invention was applied to reclaimed land soil with that of the untreated group.

[0040] Figure 8 shows the cation exchange capacity (cmol) when the hairy moss according to one embodiment of the present invention was applied to reclaimed land soil. + This is a graph comparing the untreated group ( / kg).

[0041] Figure 9 is a graph comparing the organic matter (%) content of reclaimed land soil treated with microorganisms derived from hairy moss according to one embodiment of the present invention with that of the untreated group.

[0042] Figure 10 is a graph comparing the total nitrogen content N (%) when microorganisms derived from hairy moss were treated to reclaimed land soil according to one embodiment of the present invention with that of the untreated group.

[0043] Figure 11 shows the cation exchange capacity (cmol) when the microorganism derived from the hairy moss according to one embodiment of the present invention was treated to reclaimed land soil. + This is a graph comparing the untreated group ( / kg).

[0044] A composition for improving soil environment, comprising Hypnum plumaeforme, its crushed product, or its culture.

[0045] Hereinafter, the present invention will be described in more detail with reference to the following examples. However, these examples are intended only to illustrate the present invention, and the scope of the present invention is not limited by these examples.

[0046] Throughout this specification, "%" used to indicate the concentration of a particular substance is (wt / wt)% for solid / solid, (wt / vol)% for solid / liquid, and (vol / vol)% for liquid / liquid, unless otherwise stated.

[0047] Unless otherwise specified, all numbers, values ​​and / or expressions expressing ingredients, reaction conditions and quantities of ingredients used herein are to be understood as being modified in all instances by the term "about" because these numbers are approximations that inherently reflect, among other things, the various uncertainties of measurement encountered in obtaining such values.

[0048] Additionally, when a numerical range is disclosed herein, such range is continuous and includes all values ​​from the minimum value to the maximum value inclusive, unless otherwise specified.

[0049] Also, the term "or" in this specification is intended to mean an inclusive "or" rather than an exclusive "or." That is, where a connection or use between components is not otherwise specified or clear from context, i.e., if X includes A; X includes B; or X includes both A and B, "X includes A or B" can be applied to any of these cases.

[0050]

[0051] Example 1. Obtaining microorganisms derived from hairy feather moss

[0052] After collecting hairy feather moss through legal procedures from a forest in Bukpyeong-myeon, Jeongseon-gun, Gangwon-do, microorganisms present in the collected hairy feather moss were obtained.

[0053] Specifically, 100 mg of hairy feather moss was washed with 10% NaCl in distilled water and then mixed with 10 mL of 10% NaCl. 1 mL of the mixed solution was taken, spread on an LB agar plate, and cultured in a 37°C incubator.

[0054] Afterwards, single colonies formed on the LB agar plate where the culture was completed were separated, mixed with 10 mL of new LB medium, and cultured in a shaking incubator at 37°C. The single colony mixture solution where the shaking culture was completed was spread on an LB agar plate, thereby completing the isolation of microorganisms derived from the feather moss.

[0055] Next, the microbial community was analyzed by identifying the entire isolated microorganisms through 16S rRNA sequencing (CJ Bioscience). Among the analyzed microbial communities, those expected to be useful were isolated. At this time, useful microorganisms were selected by screening tests to exclude those known to be harmful or whose beneficial properties were already known through a literature review.

[0056] The selected microorganisms were spread on agar plates and cultured at 37°C. Nine microorganisms were finally selected through single colony screening, and a single colony of each microorganism was obtained. The nine microorganism samples were delivered to the Korea Research Institute of Bioscience and Biotechnology (KCTC) for strain identification based on 16S rRNA sequencing. The results of the 16S rRNA analysis of each sample are shown in Table 1.

[0057]

[0058]

[0059] In addition, the 16S rRNA sequences analyzed for each microorganism were aligned with reference to the rRNA secondary structure, and the % similarity with the standard strain was calculated (EzBioCloud server), and a phylogenetic analysis was performed based on the derived % similarity. The phylogenetic tree created for the strains of Sample 1 and Sample 2 is shown in Fig. 1, the phylogenetic tree created for the strains of Sample 3 and Samples 5 to 7 is shown in Fig. 2, the phylogenetic tree created for the strain of Sample 4 is shown in Fig. 3, and the phylogenetic tree created for the strains of Sample 8 and Sample 9 is shown in Fig. 4.

[0060] The analysis results for the strains in each sample are as follows.

[0061] The strain of sample 1 was found to have the highest similarity of 99.66% with the standard strain of Lysinibacillus sphaericus, indicating a high probability of belonging to that species.

[0062] The strain of sample 2 was found to have the highest similarity of 100% with the standard strain of Lysinibacillus fusiformis, indicating a high probability of belonging to that species.

[0063] The strain of sample 3 was confirmed to have a similarity of 99.86% with the standard strain of Bacillus toyonensis, 99.80% with the standard strains of Bacillus mobilis and Bacillus pacificus, and 99.66 to 99.73% with the standard strains of Bacillus wiedmanii, Bacillus proteolyticus, Bacillus albus, Bacillus luti, and Bacillus fungorum. However, since these Bacillus standard strains correspond to the "Bacillus cereus clade" and accurate species identification is not possible with 16S rRNA sequencing alone, it was found that although the strain of sample 3 definitely belongs to the genus Bacillus, additional analyses such as marker gene (house-keeping gene) or whole genome analysis are necessary for accurate species identification.

[0064] The strain of sample 4 was confirmed to have the highest similarity of 99.80% and 99.66% with the standard strains of Priestia aryabhattai and Priestia megaterium, respectively, indicating a high probability of belonging to the species Priestia aryabhattai.

[0065] The strain of sample 5 was confirmed to have 100% similarity to the standard strain of Bacillus toyonensis, 99.93% similarity to the standard strains of Bacillus mobilis and Bacillus pacificus, and 99.73 to 99.80% similarity to the standard strains of Bacillus widmanii, Bacillus proteolyticus, Bacillus albus, Bacillus ruti, and Bacillus fungorum. However, since it corresponds to the "Bacillus cereus clade" like the strain of sample 3, accurate species distinction is not possible with 16S rRNA sequencing alone, so although it is certain that the strain of sample 5 belongs to the genus Bacillus, additional analyses such as marker gene or whole-genome analysis are necessary for accurate species identification.

[0066] The strain of sample 6 was confirmed to have 99.93% similarity to the standard strains of Bacillus albus and Bacillus ruti, 99.86% similarity to the standard strains of Bacillus cereus, Bacillus widmanii, and Bacillus tropicus, and 99.80% similarity to the standard strains of Bacillus proteolyticus, Bacillus paranthracis, Bacillus nitratireducens, and Bacillus fungorum. However, since it corresponds to the "Bacillus cereus clade" like the strains of samples 3 and 5, accurate species distinction is not possible with 16S rRNA sequencing alone, so although it is certain that the strain of sample 6 belongs to the genus Bacillus, additional analyses such as marker gene or whole-genome analysis are necessary for accurate species identification.

[0067] The strain of sample 7 was confirmed to have 100% similarity to the standard strain of Bacillus toyonensis, 99.93% similarity to the standard strains of Bacillus mobilis and Bacillus pacificus, and 99.80 to 99.86% similarity to the standard strains of Bacillus widmanii, Bacillus proteolyticus, Bacillus albus, Bacillus ruti, and Bacillus fungorum. However, since it corresponds to the "Bacillus cereus clade" like the strains of samples 3, 5, and 6, accurate species distinction is not possible with 16S rRNA sequencing alone, so although it is certain that the strain of sample 7 belongs to the genus Bacillus, additional analyses such as marker gene or whole-genome analysis are necessary for accurate species identification.

[0068] The strain of sample 8 was confirmed to have 100% similarity to the standard strain of Paenibacillus taichungensis, indicating a high probability of belonging to that species.

[0069] The strain of sample 9 was confirmed to have 99.59% similarity to the standard strains of Paenibacillus amylolyticus and Paenibacilus tundrae, confirming its belonging to the genus Paenibacillus. However, further analysis, such as marker gene or whole-genome analysis, is required for accurate species identification.

[0070]

[0071] Example 2. Evaluation of soil environment improvement capacity

[0072] The soil environment improvement ability of the hairy moss collected according to Example 1 and microorganisms derived from the hairy moss was evaluated. A schematic process for evaluating the soil environment improvement ability of the hairy moss and microorganisms derived from the hairy moss is shown in Figure 5.

[0073]

[0074] 2-1. Evaluation of the soil environment improvement ability of hairy moss

[0075] To examine whether hairy feather moss is suitable for use as an effective ingredient in a composition for improving soil environment, the soil environment improvement ability of hairy feather moss was evaluated.

[0076] Specifically, after collecting the reclaimed soil from Taean-gun, Chungcheongnam-do as a target for soil environment improvement evaluation, 9 g of crushed hairy feather moss and 100 mL of distilled water were sprayed on 900 g of the collected soil at weekly intervals and cultured for approximately 1 month.

[0077] Then, the control group that did not treat any substance for the soil and the experimental group that treated the hairy moss were set as evaluation groups, and soil component analysis was conducted for each group (AT Analysis Center). The analysis results are shown in Table 2 below, and the organic matter (%), total nitrogen N (%), and cation exchange capacity (cmol) of the control and experimental groups + The results of comparing ( / kg) are shown in Figures 6 to 8 and Tables 3 to 5.

[0078] Analysis items Control group Experimental group (feather moss treatment) Organic matter (%) 0.64 2.65 N (%) 0.02 8 0.079 Available P2O5 (mg / kg) 8.29 10.14 Exchangeable K (cmol + / kg)0.0680.19Exchangeable Ca (cmol + / kg)0.411.22 Substitutable Mg(cmol + / kg)0.290.66 Substitutable Na (cmol + / kg)0.0860.12 Cation exchange capacity (cmol + / kg)2.104.20pH[1:5]6.836.46Electrical Conductivity[1:5] (dS / m)0.0580.068NaCl (%)0.0480.0062Moisture (%)5.535.31Lead (mg / kg)0.0220.024Chromium (mg / kg)Not DetectedNot DetectedCopper (mg / kg)2.672.80Nickel (mg / kg)Not DetectedNot DetectedZinc (mg / kg)Not Detected0.57

[0079]

[0080] Untreated groupExperimental groupOrganic matter content (%)0.642.65

[0081]

[0082] Untreated group, experimental group, total nitrogen N (%) 0.028 0.079

[0083]

[0084] Untreated group Experimental group Cation exchange capacity (cmol + / kg)2.104.20

[0085]

[0086] As a result of the analysis, the soil treated with hairy moss had about 4.1 times more organic matter (%), about 2.8 times more total nitrogen (N) and about 2.8 times more cation exchange capacity (cmol) than the untreated group. + / kg) was found to have increased by about 2 times, which meant that the conditions required for plant growth could be supplemented by treating the reclaimed land soil with incomplete growth with hairy feather moss.

[0087]

[0088] 2-2. Evaluation of the soil environment improvement ability of microorganisms derived from hairy moss

[0089] To examine whether microorganisms derived from Pleurotus eryngii could be used as effective ingredients in soil environment improvement compositions, the soil environment improvement capacity of Pleurotus eryngii-derived microorganisms was evaluated. The strain of Sample 4, which was identified as a Priestia ariabatai strain and was the only one among nine samples to have a phylogenetic tree constructed based on 16S rRNA sequence analysis, was utilized.

[0090] Specifically, as a subject of soil environment improvement evaluation, reclaimed soil from Taean-gun, Chungcheongnam-do was collected, and 100 mL of a culture solution containing the strain of sample 4 obtained according to Example 1 was sprayed on 900 g of the collected soil at weekly intervals, and cultured for approximately 1 month.

[0091] Then, a control group was set up in which 100 mL of the liquid medium used for microbial culture was sprayed at one-week intervals on the soil, and an experimental group was set up in which the strain culture of sample 4 was sprayed. Then, soil component analysis was performed on each group (AT Analysis Center). The analysis results are shown in Table 6 below, and the organic matter (%), total nitrogen N (%), and cation exchange capacity (cmol) of the control and experimental groups are + The results of comparing / kg) are shown in Figures 9 to 11 and Tables 7 to 9.

[0092] Analysis items Control group Experimental group (strain treatment of sample 4) Organic matter (%) 0.66 0.87 N (%) 0.04 4 0.063 Available P2O5 (mg / kg) 20.18 15.74 Exchangeable K (cmol + / kg)0.210.21Exchangeable Ca (cmol + / kg)0.400.52 Substitutable Mg(cmol + / kg)0.360.46 Substitutable Na (cmol + / kg)1.120.48 Cation exchange capacity (cmol + / kg)2.603.10pH[1:5]6.126.23

[0093]

[0094] Control group, experimental group, organic matter content (%) 0.660.87

[0095]

[0096] Control group experimental group total nitrogen N (%) 0.0440.063

[0097]

[0098] Control group Experimental group Cation exchange capacity (cmol + / kg)2.603.10

[0099]

[0100] As a result of the analysis, the organic matter (%) was approximately 1.3 times higher, the total nitrogen content N (%) was approximately 1.4 times higher, and the cation exchange capacity (cmol + / kg) was found to have increased by about 1.2 times, which meant that the conditions required for plant growth could be supplemented by treating microorganisms derived from hairy feather moss in reclaimed soil with incomplete growth.

[0101] From the above experimental results, it was thought that the strain of Pseudomonas araliabataei derived from Pseudomonas araliabataei could improve the soil environment suitable for plant growth by improving the organic matter content, nitrogen content, and cation exchange capacity of degraded or polluted soil.

[0102] While the present invention has been described in detail through representative examples above, those skilled in the art will understand that various modifications to the above-described embodiments are possible without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be defined not only by the claims described below but also by all changes or modifications derived from the claims and equivalent concepts.

[0103] The purpose of the present invention is to provide a composition for improving soil environment containing Hypnum plumaeforme.

[0104] Another object of the present invention is to provide a microorganism isolated from a hairy feather moss.

[0105] Another object of the present invention is to provide a composition for improving soil environment containing microorganisms derived from hairy moss.

[0106] Another object of the present invention is to provide a method for improving the soil environment through a composition for improving the soil environment.

[0107]

Claims

1. A composition for improving soil environment, comprising Hypnum plumaeforme, its crushed product, or its culture.

2. A composition for improving soil environment, comprising a microorganism derived from feather moss or a culture thereof.

3. A composition according to claim 2, wherein the microorganism is Priestia aryabhattai.

4. Method for improving soil environment including the following steps: A treatment step of treating soil with a composition for improving soil environment, the composition including at least one selected from the group consisting of hairy moss, a pulverized product of hairy moss, a culture of hairy moss, a microorganism derived from hairy moss, and a culture of a microorganism derived from hairy moss.

5. In the fourth paragraph, the treatment step is a method in which a moss composition including at least one selected from the group consisting of a hairy moss, a pulverized hairy moss, and a culture of a hairy moss, and a microbial composition including at least one selected from the group consisting of a hairy moss-derived microorganism, and a culture of a hairy moss-derived microorganism are complexly treated.

6. In the fourth paragraph, the method, A soil cultivation step in which soil treated with a composition for improving soil environment is cultivated for a predetermined period of time; A method comprising:

Images