Composite polyester fiber, polyester fiber cloth, fiber cloth carrier, and preparation method and use

Abstract

A composite polyester fiber, and a polyester fiber cloth, a fiber cloth carrier and a cell culture carrier which comprise the composite polyester fiber, and a preparation method and use. The composite polyester fiber has a sheath-core structure, wherein the mass ratio of a core layer material to a sheath layer material is x, 1.5≤x≤4, and the average diameter D of the composite polyester fiber is 10-40 μm. When the polyester fiber cloth, the fiber cloth carrier or the cell culture carrier which are prepared from the composite polyester fiber are used for cell culture, the glycometabolism capability of cells can be improved.

Description

Composite polyester fiber, polyester fiber cloth, fiber cloth carrier, and preparation method and application thereof Technical Field

[0001] The present invention relates to the technical field of composite polyester fibers, and in particular to a composite polyester fiber, a polyester fiber cloth made of the composite polyester, a fiber cloth carrier, and a preparation method and application thereof. Background Art

[0002] Currently, large-scale animal cell culture technology is widely used in the production of various cells and cell products, including biological products such as enzymes, growth factors, vaccines, and antibodies. Most animal cells tend to grow adherently, so when cultured in vitro, they typically require a support for growth. This support is commonly referred to by those skilled in the art as a cell culture support. Current cell culture supports include granular supports with a diameter of 2-11 mm, porous spherical supports, or sheet-like fiber supports. Current microcarriers include macroporous microcarriers made of silk fibroin and chitosan, and hybrid microcarriers made of chitosan and gelatin. Spherical microcarriers suffer from the problem that cells can only adhere to the surface and have a low area-to-volume ratio. While porous microcarriers contain pores that facilitate cell attachment, these pores are easily blocked, which in turn affects oxygen and material exchange. Currently, research on fiber scaffold supports is limited both domestically and internationally. The main fiber scaffolds are electrospun materials, which are primarily used for in vivo transplantation in tissue engineering, while pure in vitro large-scale cell culture is relatively rare. The structural characteristics of electrospun fiber scaffolds are that the electrospun fibers have a nanoscale microstructure with many voids, which can simply mimic the distribution of extracellular matrix fibers for cell attachment and growth. However, the voids are small, and cells can easily block the relevant voids after attaching in sheets, which severely restricts material exchange. In addition, the cells easily float on the surface of the culture medium, which is not conducive to the contact between cells and the culture medium.

[0003] The smallest component unit of the carrier for cell culture is fiber. The fiber can be especially polyester fiber.

[0004] Polyester fiber is a product made by applying polyester in fiber form. The most commonly used polyester material is polyethylene terephthalate (PET), although other polyesters such as polybutylene terephthalate and polylactic acid are also used. The methods for preparing polyester into fibers can be roughly divided into melt spinning and solution spinning. Solution spinning is further divided into dry spinning and wet spinning, depending on whether the solvent used is volatile. Melt spinning can be simply divided into direct spinning, mixed spinning, and composite spinning. Composite spinning can produce composite fibers.

[0005] Composite fibers are chemical fibers made from two or more polymers, or the same polymer with different properties, through a composite spinning process. Depending on the distribution of the two polymers across the cross-section, they can be categorized as sheath-core, segmented, island-in-the-sea, and parallel types.

[0006] However, the prior art lacks polyester fibers, polyester fiber cloths, and fiber cloth carriers for efficient cell culture, and lacks methods for obtaining the polyester fibers and preparing polyester fiber cloths / fiber cloth carriers to achieve efficient cell culture.

[0007] Summary of the Invention

[0008] One of the objectives of the present invention is to overcome the low cell survival rate of existing cell culture carriers when used for cell culture. The present invention provides a composite polyester fiber with excellent thickness uniformity, a polyester fiber cloth made from the composite polyester, a fiber cloth carrier, and methods for preparing and using the same. The composite polyester fiber, fiber cloth, and carrier prepared from the composite polyester fiber of the present invention can significantly improve cell survival rate and accelerate cell proliferation when used for cell culture.

[0009] During the research process, the inventors found that by controlling the average diameter D of the composite polyester fiber, the standard deviation of the diameter of the composite polyester fiber, the dosage ratio of the core layer and the sheath material, the melting point difference of the core layer and the sheath material, the intrinsic viscosity difference and the standard deviation, and by using a high-precision spinneret, preferably making the dosage ratio of the core layer and the sheath material and the average diameter D of the fiber and the standard deviation of the diameter meet the conditions of the present invention, a composite polyester fiber with good thickness uniformity can be obtained, which can then be used to manufacture polyester fiber cloth and fiber cloth carrier for cell culture.

[0010] Therefore, in a first aspect, the present invention provides a composite polyester fiber having a core-sheath structure; wherein the mass ratio of the core layer material to the sheath layer material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, more preferably 2≤x≤3; and wherein the average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, more preferably 18-30 μm, and the diameter standard deviation is ≤2.2 μm, preferably ≤2.0 μm, more preferably ≤1.8 μm.

[0011] A second aspect of the present invention provides a method for preparing the composite polyester fiber, which comprises: melting the core layer material and the skin layer material separately to obtain a melt, and spinning the melt to obtain the composite polyester fiber.

[0012] A third aspect of the present invention provides a polyester fiber cloth, which comprises the composite polyester fiber or the composite polyester fiber prepared by the above method; for example, the polyester fiber cloth can be prepared by using the composite polyester fiber.

[0013] A fourth aspect of the present invention provides a method for preparing the above-mentioned polyester fiber cloth, which comprises laying the composite polyester fibers and hot pressing to obtain the polyester fiber cloth.

[0014] A fifth aspect of the present invention provides a fiber cloth carrier for cell culture, wherein the fiber cloth carrier is made of the above-mentioned polyester fiber cloth.

[0015] A sixth aspect of the present invention provides a method for preparing the above-mentioned fiber cloth carrier, wherein the method comprises: pretreating and / or sterilizing the above-mentioned polyester fiber cloth.

[0016] A seventh aspect of the present invention provides use of the composite polyester fiber in preparing a fiber cloth carrier for cell culture.

[0017] An eighth aspect of the present invention provides a use of the polyester fiber cloth or fiber cloth carrier in cell culture.

[0018] The ninth aspect of the present invention provides a method for culturing cells, which comprises: sterilizing the above-mentioned polyester fiber cloth and placing it in a culture medium as a cell carrier, or placing the above-mentioned fiber cloth carrier in a culture medium as a cell carrier, and inoculating cells on the carrier for in vitro culture; preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0019] Through the above technical solution, the present invention achieves the following beneficial effects:

[0020] The composite polyester fiber of the present invention has a small diameter and a small standard deviation of the diameter. When used in cell culture, the composite polyester fiber and the polyester fiber cloth or fiber cloth carrier made therefrom can significantly improve the sugar metabolism ability of cells. A higher sugar metabolism ability indicates a higher cell reproduction rate and survival rate (only living cells can metabolize sugar; dead cells cannot). Therefore, the composite polyester fiber and the fiber cloth and carrier made therefrom can achieve better results when used in cell culture. Therefore, the present invention can significantly improve cell survival rate and accelerate cell reproduction. DETAILED DESCRIPTION

[0021] The endpoints of the ranges and any values ​​disclosed herein are not limited to the precise ranges or values, and these ranges or values ​​should be understood to include values ​​close to these ranges or values. For numerical ranges, the endpoints of each range, the endpoints of each range and individual point values, and the individual point values ​​can be combined with each other to obtain one or more new numerical ranges, which should be considered to be specifically disclosed herein.

[0022] A first aspect of the present invention provides a composite polyester fiber having a sheath-core structure; wherein the mass ratio of the core layer material to the sheath layer material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, more preferably 2≤x≤3; and wherein the average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, more preferably 18-30 μm, and the diameter standard deviation is ≤2.2 μm, preferably ≤2.0 μm, more preferably ≤1.8 μm.

[0023] In the present invention, preferably, the fiber cloth prepared by using a sheath-core structure composite polyester fiber that meets the above-mentioned mass ratio, average diameter and diameter standard deviation can improve the cell reproduction rate and survival rate, so that the cell sugar metabolism value is above 15 g / day; and when the average diameter and diameter standard deviation of the polyester fiber deviate from the above range, the cell sugar metabolism value is below 10 g / day.

[0024] In the present invention, the core layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably homopolyethylene terephthalate, and the skin layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably copolyethylene terephthalate.

[0025] In the present invention, the amount of the core layer material is greater than that of the sheath material. The mass ratio (weight ratio) of the core layer material to the sheath material can be 1.5, 1.6, 1.7, 1.8, 1.9, 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9, 4, or a range consisting of any two of the foregoing. Preferably, it is 2-3.6, and more preferably 2-3. By limiting the weight ratio of the core layer material to the sheath material within the preferred range, a smaller standard deviation of the diameter of the composite polyester fiber can be obtained.

[0026] In the present invention, the skin-to-core mass ratio can be determined by the following method: about 10 mg of fiber or fiber cloth sample is dissolved in 50-100 mL of o-chlorophenol at 120-140° C., and then measured using liquid chromatography, such as high-temperature liquid chromatography, with a non-polar or weakly polar filler such as C18 (ODS); the mobile phase of the liquid chromatography can use a solvent with a strong polarity, such as a mixture of trifluoroacetic acid, phenol and 1,1,2,2-tetrachloroethane (mass ratio 60:40), a mixture of phenol and chloroform (volume ratio 1:1), etc.; two main peaks will appear, namely the skin material peak and the core material peak; the peak area of ​​each peak is integrated, and the ratio represents the mass ratio of the skin material to the core material, that is, the skin-to-core mass ratio. For example, when copolyester uses cyclohexanedimethanol as a comonomer, the homopolyester peaks first and the copolyester peaks later; when copolyester uses isophthalic acid as a comonomer, the copolyester peaks first and the homopolyester peaks later.

[0027] In the present invention, the average diameter of the composite polyester fiber can be 10 μm, 15 μm, 17 μm, 18 μm, 20 μm, 22 μm, 24 μm, 26 μm, 28 μm, 30 μm, 32 μm, 40 μm, or a range consisting of any two of the above.

[0028] The standard deviation of the diameter of the composite polyester fiber can be 2.2 μm, 2.1 μm, 2.0 μm, 1.9 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, and a range formed by any two of the above points, for example, 0.1-2.2 μm.

[0029] In the present invention, the test method for the standard deviation of the diameter of the composite polyester fiber is as follows: randomly select a small section from 50 composite polyester fibers, take a scanning electron microscope (SEM) photograph, and measure the diameter of each fiber from the photograph (measure each fiber once) to an accuracy of 0.1 μm. The arithmetic mean of the diameters of the 50 fibers is the average diameter of the composite polyester fiber. The standard deviation of these diameter values ​​is then calculated using the standard deviation calculation method. The calculation formula for the diameter standard deviation is as follows:

[0030] Where n is the number of composite polyester fiber samples (i.e. 50), i is the fiber sample number, and d i is the diameter of the fiber numbered i, It is the arithmetic mean of the diameters of 50 fiber samples.

[0031] In some embodiments of the present invention, the composite polyester fiber is a composite polyester fiber having a length greater than 150 mm.

[0032] According to the present invention, preferably, the intrinsic viscosity of the skin material is 0.01-0.18 dL / g higher than the intrinsic viscosity of the core material, preferably 0.01-0.15 dL / g, and the standard deviation of the intrinsic viscosities of the core material and the skin material is ≤0.02 dL / g, preferably ≤0.015 dL / g.

[0033] In the present invention, the intrinsic viscosity of polyester is tested using the intrinsic viscosity test method described in GBT14190-2017. The standard deviation of the intrinsic viscosity is determined by randomly selecting six samples, testing their intrinsic viscosity according to the intrinsic viscosity test method described in GBT14190-2017, and calculating the arithmetic mean of the measured intrinsic viscosity values ​​as the intrinsic viscosity of the polyester. A smaller standard deviation of the intrinsic viscosity indicates a higher uniformity of the intrinsic viscosity across the same batch of materials. The standard deviation of the intrinsic viscosity is calculated using the following formula:

[0034] Where m is the number of samples (i.e. 6), i is the sample number, and η i is the intrinsic viscosity of sample numbered i, It is the arithmetic mean of the intrinsic viscosity of 6 samples.

[0035] According to the present invention, the standard deviation of the intrinsic viscosity of the core layer material and the skin layer material can be independently 0.02 dL / g, 0.018 dL / g, 0.015 dL / g, 0.01 dL / g, 0.009 dL / g, 0.008 dL / g, 0.007 dL / g, 0.006 dL / g, 0.005 dL / g, 0.004 dL / g, 0.003 dL / g, 0.002 dL / g, 0.001 dL / g, 0.0001 dL / g, and the range formed by any two of the above points, for example, 0.0001-0.02 dL / g.

[0036] According to the present invention, the melting point of the core layer material is more than 20°C higher than the melting point of the skin layer material; for example, 20°C, 22°C, 24°C, 25°C, 30°C, 40°C, 50°C, 60°C, 80°C, 100°C, 110°C, 120°C, 130°C, 140°C, 150°C, 160°C, and a range consisting of any two of the above points, for example, 20-160°C; preferably, the melting point of the core layer material is at least 22°C higher than the melting point of the skin layer material, more preferably at least 24°C higher.

[0037] In the present invention, the melting points of the core layer material and the skin layer material are measured by differential scanning calorimetry. Specifically, the sample is heated from 20°C to 300°C at a heating rate of 10°C / min, and the peak temperature of the endothermic peak between 100°C and 260°C is taken as the melting point.

[0038] According to the present invention, surprisingly, the inventors have further discovered that when a fiber cloth prepared from polyester fibers having a mass ratio x of the core layer material to the skin layer material and an average diameter D of the polyester fibers satisfying the following formula (1) is used for cell culture, it can further improve the sugar metabolism capacity of cells:

[0039] The numerical value of the average diameter D of the composite polyester fiber used in formula (1) is in μm. For example, if the average diameter D is 20 μm, the numerical value of D in formula (1) is 20.

[0040] Further preferably, when the mass ratio of the core layer material to the skin layer material of the composite polyester fiber is 2-3 and satisfies formula (1), the sugar metabolism ability of cells can be further improved when the composite polyester fiber and the polyester fiber cloth or carrier prepared therefrom are used to culture cells.

[0041] According to the present invention, in some embodiments, preferably, the core layer material is homopolyethylene terephthalate. More preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8 dL / g, such as 0.6 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.8 dL / g, and the range of any two of the above. The melting point is above 240°C, for example, 240°C, 245°C, 248°C, 250°C, 255°C, 260°C, 265°C, 270°C, 280°C, 290°C, 300°C, 320°C, 350°C, 380°C, and a range consisting of any two of the above points, more preferably 245-280°C.

[0042] According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the homopolyethylene terephthalate is ≤0.015 dL / g, preferably ≤0.01 dL / g, more preferably ≤0.005 dL / g.

[0043] According to the present invention, in some embodiments, preferably, the skin material is copolyethylene terephthalate; more preferably, the intrinsic viscosity of the copolyethylene terephthalate is 0.601-0.9 dL / g, such as 0.61 dL / g, 0.62 dL / g, 0.64 dL / g, 0.66 dL / g, 0.68 dL / g, 0.70 dL / g, 0.72 dL / g, 0.74 dL / g, 0.76 dL / g, 0.78 dL / g, 0.80 dL / g, 0.82 dL / g, 0.84 dL / g, 0.86dL / g, 0.88dL / g, 0.9dL / g and the range formed by any two of the above points, preferably 0.61-0.9dL / g, more preferably 0.61-0.82dL / g, and the melting point is below 240°C, for example 240°C, 230°C, 235°C, 220°C, 200°C, 190°C, 180°C, 170°C, 160°C, 150°C, 145°C, 140°C, 135°C, 130°C, 120°C, and the range formed by any two of the above points, more preferably 130-240°C.

[0044] According to the present invention, preferably, the standard deviation of the intrinsic viscosity of the copolyethylene terephthalate is ≤0.015 dL / g, preferably ≤0.01 dL / g.

[0045] According to the present invention, preferably, the copolyethylene terephthalate comprises or consists of structural units derived from terephthalic acid, ethylene glycol, and comonomer Y. The content of the structural units derived from comonomer Y in the copolyethylene terephthalate may be 0.6-20 mol%, for example, 0.6 mol%, 0.75 mol%, 1 mol%, 1.5 mol%, 3 mol%, 5 mol%, 10 mol%, 15 mol%, 18 mol%, 20 mol%, or any range consisting of any two of the foregoing, preferably 0.6-18 mol%, and more preferably 0.7-15 mol%. In the present invention, the content of the structural units derived from comonomer Y refers to the percentage of the molar amount of the structural units derived from comonomer Y to the total molar amount of the structural units derived from terephthalic acid, ethylene glycol, and comonomer Y.

[0046] According to the present invention, there is no particular limitation on the type of the comonomer Y, as long as the melting point and intrinsic viscosity difference between the skin material and the core material and the standard deviation of the intrinsic viscosity can meet the requirements. Preferably, the comonomer Y can be selected from at least one of a dicarboxylic acid, a diol and a tetraol. More preferably, the dicarboxylic acid is a dicarboxylic acid containing a benzene ring, more preferably isophthalic acid and / or phthalic acid. More preferably, the diol is a diol having a carbon number of 3-20, more preferably at least one of butanediol, hexanediol, cyclohexanedimethanol and pentanediol (e.g., neopentanediol). More preferably, the tetraol is a tetraol having a carbon number of 3-20. In the present invention, the carbon number of the diol or tetraol can be independently 3, 4, 5, 6, 7, 8, 9, 10, 12, 15 or 20.

[0047] In the present invention, the comonomer Y may also be selected from acid monomers in other forms such as anhydrides, acid chlorides, methanol esters, and ethanol esters, and alcohol monomers in other forms such as ethers, hemiacetals, and acetals.

[0048] According to the present invention, preferably, the Sb content in the core layer material and the sheath layer material of the polyester fiber is less than 20 ppm. In the present invention, "ppm" refers to the "weight" content.

[0049] A second aspect of the present invention provides a method for preparing the composite polyester fiber, which comprises: melting the core layer material and the skin layer material separately to obtain a melt, and spinning the melt to obtain the composite polyester fiber.

[0050] In the present invention, the shape of each spinneret of the spinneret used in the spinning process is two concentric circles, and the difference between the maximum diameter and the minimum diameter of all inner circles of the spinneret (also known as the range, that is, the pore size of the largest hole minus the pore size of the smallest hole) is ≤2μm, and the difference between the maximum diameter and the minimum diameter of all outer circles of the spinneret (also known as the range, that is, the pore size of the largest hole minus the pore size of the smallest hole) is ≤2μm. In the present invention, the difference between the maximum diameter and the minimum diameter of all inner circles and the difference between the maximum diameter and the minimum diameter of all outer circles can each independently be 0.1μm, 0.5μm, 1.5μm, 2μm, and a range consisting of any two of the above points, for example, 0.1-2μm.

[0051] In the present invention, it can be understood that the core layer material forms the core layer structure of the composite polyester fiber after passing through the inner circle of the spinneret, and the skin layer material forms the skin structure of the composite polyester fiber after passing through the outer circle of the spinneret, and then after cooling and stretching, a composite polyester fiber with a skin-core structure is formed.

[0052] According to the present invention, preferably, the inner diameter of the inner circle of the spinneret is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm. In the present invention, the precise aperture of the spinneret is measured by a profilometer. For example, a Bruker Contour GT profilometer can be used.

[0053] According to the present invention, preferably, the process of spinning the melt to obtain a composite polyester fiber having a sheath-core structure comprises the following steps:

[0054] (1) feeding the melt (core layer material melt and skin layer material melt) into a spinneret for spinning to obtain a filamentous melt having a skin-core structure;

[0055] (2) cooling the filamentous melt having a skin-core structure to obtain a spun fiber; and

[0056] (3) The spun fibers are stretched to obtain composite polyester fibers.

[0057] According to the present invention, preferably, the core layer material melt is obtained by melting the core layer material at 220-280°C (eg, 220°C, 240°C, 260°C, 280°C, and a range consisting of any two of the above).

[0058] According to the present invention, preferably, the skin material melt is obtained by melting the skin material at 200-280°C (eg, 200°C, 220°C, 240°C, 260°C, 280°C, and a range consisting of any two of the above).

[0059] In the present invention, it is understood that the temperature of the core layer material melt and the skin layer material melt refers to the temperature of the spinneret when the core layer material melt and the skin layer material melt are extruded from the spinneret outlet.

[0060] According to the present invention, preferably, side blowing is used for cooling, wherein the wind speed of the side blowing can be 1.5-2m / s, such as 1.5m / s, 1.6m / s, 1.7m / s, 1.8m / s, 1.9m / s, 2m / s, and the range formed by any two of the above points, more preferably 1.5-1.8m / s; and the temperature is 15-30℃, such as 15℃, 20℃, 25℃, 30℃, and the range formed by any two of the above points, more preferably 15-20℃.

[0061] According to the present invention, stretching is preferably performed using a high-speed airflow, wherein the flow rate of the high-speed airflow is 2500-5500 m / min, for example, preferably 3000-5000 m / min. Advantageously, stretching using a high-speed airflow can further preferentially orient the fiber structure along the processing direction of the fiber, thereby achieving more uniform fiber thickness and improving the mechanical properties of the fiber.

[0062] According to the present invention, preferably, the stretching method includes at least one of a circular tube stretching method and a slit stretching method.

[0063] The circular tube stretching method of the present invention refers to a method in which a plurality of spun fibers (e.g., 2-20 fibers) are fed into a long circular tube and stretched by air at a constant flow rate within the tube. In the circular tube stretching method, the air flow rate can be 2500-5500 m / min, preferably 3000-5000 m / min.

[0064] According to the present invention, the inner diameter of the circular tube used in the circular tube method is preferably 0.5-5 cm, and the distance between two adjacent circular tubes is 0.5-10 cm. The distance between two adjacent circular tubes refers to the distance between the centers of the two adjacent circular tubes. The length of the circular tube can be 0.1-2 m. The length of the circular tube refers to the distance along the direction of fiber ejection.

[0065] According to the present invention, the slits in the slit drawing method preferably have a spacing of 0.3-3 cm and a width of 10-60 cm. It is understood that the slit width refers to the distance parallel to the long side of the spinneret. The slit length can be 10-60 cm. The slit length refers to the distance along the direction of fiber ejection.

[0066] According to the present invention, preferably, a high-speed airflow is used in the slit to stretch the spun fibers, wherein the flow rate of the high-speed airflow is 2500-5500 m / min, preferably 3000-5000 m / min.

[0067] A third aspect of the present invention provides a polyester fiber cloth comprising the composite polyester fiber described above or the composite polyester fiber produced by the method described above. In some embodiments, the polyester fiber cloth is made from the composite polyester fiber described above, for example, the polyester fiber cloth is prepared from the composite polyester fiber described above. In some embodiments, the polyester fiber cloth may comprise only the composite polyester fiber described above and not other fibers. According to the present invention, the polyester fiber cloth is preferably a nonwoven fabric.

[0068] According to the present invention, preferably, the polyester fiber cloth has a thickness of 0.35-0.6 mm, preferably 0.4-0.5 mm, and an area density of 70-160 g / m 2 , preferably 80-130g / m 2 .

[0069] In the present invention, the thickness of the polyester fiber cloth can be 0.35mm, 0.4mm, 0.41mm, 0.42mm, 0.43mm, 0.44mm, 0.45mm, 0.46mm, 0.47mm, 0.48mm, 0.49mm, 0.50mm, 0.51mm, 0.52mm, 0.53mm, 0.54mm, 0.55mm, 0.56mm, 0.57mm, 0.58mm, 0.59mm or 0.60mm, or a range consisting of any two of the above. The surface density of the polyester fiber can be 70g / m 2 , 80g / m 2 , 85g / m 2 , 90g / m 2 , 95g / m 2 、 100g / m 2 , 105g / m 2 , 110g / m 2 , 115g / m 2 , 120g / m 2 , 125g / m 2 , 130g / m 2 , 135g / m 2 , 140g / m 2 , 150g / m 2 or 160g / m 2 , and the range consisting of any two of the above points.

[0070] Generally, the polyester fiber cloth of the present invention can be prepared from the composite polyester fiber of the present invention by various methods generally known in the art.

[0071] In the present invention, the standard deviation of the fiber diameters in the polyester fiber cloth is ≤ 2.4 μm, preferably ≤ 2.2 μm, and more preferably ≤ 2.0 μm. The standard deviation of the fiber diameters in the polyester fiber cloth is measured by taking a scanning electron microscope (SEM) photograph of the polyester fiber cloth and measuring the diameters of 50 randomly selected fibers from the photograph (each fiber is measured once) to an accuracy of 0.1 μm. The standard deviation of these diameter values ​​is then calculated using the standard deviation calculation method. The diameter standard deviation is calculated using the following formula:

[0072] Where n is the number of composite polyester fiber samples (i.e. 50), i is the fiber sample number, and d i is the diameter of the fiber numbered i, It is the arithmetic mean of the diameters of 50 fiber samples.

[0073] The standard deviation of the fiber diameters in the polyester fiber cloth may be 2.4 μm, 2.3 μm, 2.2 μm, 2.1 μm, 2.0 μm, 1.9 μm, 1.8 μm, 1.6 μm, 1.4 μm, 1.2 μm, 1 μm, 0.8 μm, 0.6 μm, 0.4 μm, 0.2 μm, 0.1 μm, or a range consisting of any two of the above points, for example, 0.1-2.4 μm.

[0074] The method for testing the surface density of the fiber cloth in the present invention is: using a 1 / 10,000 balance to measure the surface density of 1 cm 2 The fiber cloth of the desired area is weighed, and the mass of the fiber cloth is then divided by its area. In the present invention, the thickness of the fiber cloth is measured using a thickness gauge, such as a dial-type thickness gauge. For example, a Japanese Mitutoyo 547-313 thickness gauge can be used.

[0075] A fourth aspect of the present invention provides a method for preparing the above-mentioned polyester fiber cloth, which comprises laying and hot-pressing the composite polyester fiber of the present invention to obtain the polyester fiber cloth.

[0076] According to the present invention, preferably, the temperature of the hot pressing is 180-220°C.

[0077] Instead of using long fibers, the method may also be such that a slurry of short fibers containing polyester fibers is wet-laid and heat-treated in sequence.

[0078] In the method, the length of the short polyester fibers may be 1 mm, 2 mm, 3 mm, 4 mm, 5 mm, 6 mm, 7 mm or 8 mm, and a range consisting of any two of the above.

[0079] Preferably, the length of the short polyester fibers is 1-4 mm, more preferably 2-3 mm.

[0080] Preferably, the polyester fiber content in the polyester fiber-containing slurry is 5-30% by weight, for example, 5% by weight, 8% by weight, 10% by weight, 12% by weight, 14% by weight, 16% by weight, 18% by weight, 20% by weight, 25% by weight, 30% by weight, and ranges consisting of any two of the foregoing, more preferably 10-20% by weight.

[0081] According to the present invention, preferably, the slurry containing polyester fibers further comprises a dispersant, and the dispersant is at least one of polyethylene glycol, fatty alcohol and polyacrylamide.

[0082] According to the present invention, preferably, the content of the dispersant in the slurry containing polyester fibers is 0.05-2 weight %, for example, 0.05 weight %, 0.5 weight %, 0.8 weight %, 0.9 weight %, 1 weight %, 1.1 weight %, 1.2 weight %, 1.3 weight %, 1.4 weight %, 1.5 weight %, 1.8 weight %, 2 weight %, and ranges consisting of any two of the above.

[0083] According to the present invention, preferably, the dispersant is a combination of polyethylene glycol, fatty alcohol and polyacrylamide. More preferably, the weight ratio of polyethylene glycol, fatty alcohol and polyacrylamide is 1:0.5-2:0.5-2, and more preferably 1:1-1.2:1-1.2.

[0084] In the present invention, the weight ratio of polyethylene glycol to fatty alcohol can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, or a range consisting of any two of the above.

[0085] In the present invention, the weight ratio of polyethylene glycol to polyacrylamide can be 1:0.5, 1:0.7, 1:0.9, 1:1, 1:1.1, 1:1.2, 1:1.3, 1:1.5, 1:2, or a range consisting of any two of the above.

[0086] According to the present invention, preferably, the molecular weight of the polyethylene glycol is 400-4000 g / mol (for example, 400 g / mol, 500 g / mol, 600 g / mol, 700 g / mol, 800 g / mol, 900 g / mol, 1000 g / mol, 1500 g / mol, 1600 g / mol, 1700 g / mol, 2000 g / mol, and a range consisting of any two of the above points), more preferably 500-2000 g / mol.

[0087] According to the present invention, preferably, the fatty alcohol is a fatty alcohol having 3-12 carbon atoms, more preferably, the number of hydroxyl groups in the fatty alcohol is 1-3; further preferably, the fatty alcohol is at least one of octanol, heptanol, hexanol, pentanol and butanol.

[0088] According to the present invention, preferably, the molecular weight of the polyacrylamide is 50,000-5,000,000 g / mol (for example, 50,000 g / mol, 100,000 g / mol, 150,000 g / mol, 180,000 g / mol, 200,000 g / mol, 220,000 g / mol, 240,000 g / mol, 250,000 g / mol, 300,000 g / mol, 400,000 g / mol, 1,000,000 g / mol, 5,000,000 g / mol, and a range consisting of any two of the above points), more preferably 200,000-250,000 g / mol.

[0089] In the present invention, unless otherwise specified, molecular weights are number average molecular weights.

[0090] According to the present invention, preferably, the temperature of the heat treatment is 180-220°C.

[0091] A fifth aspect of the present invention provides a fiber cloth carrier for cell culture, wherein the fiber cloth carrier is made of the above-mentioned polyester fiber cloth. In some embodiments, preferably, the fiber cloth carrier is a sheet-shaped fiber cloth carrier.

[0092] In the present invention, the polyester fiber cloth of the present invention may be pretreated and / or sterilized to prepare the fiber cloth carrier.

[0093] Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution.

[0094] Preferably, the sterilization includes at least one of ethylene oxide sterilization, high-temperature steam sterilization and irradiation sterilization, preferably high-temperature steam sterilization.

[0095] According to the present invention, preferably, the conditions for high-temperature steam sterilization include: a temperature of 115-135°C, such as 115°C, 120°C, 125°C, 130°C, 135°C, and a range consisting of any two of the above points, and a time of 10-60min, such as 10min, 20min, 30min, 40min, 50min, 60min, and a range consisting of any two of the above points.

[0096] According to the present invention, preferably, the conditions for irradiation sterilization include: irradiating the pretreated fiber cloth with high-energy electron rays, with an irradiation dose of 5-30 kGy and an irradiation time of less than 10 seconds.

[0097] According to the present invention, preferably, the fiber cloth is pretreated before sterilization. In some embodiments, the pretreatment can be performed by pretreating the fiber cloth with a hydrogen peroxide solution.

[0098] According to the present invention, preferably, the concentration of H2O2 in the hydrogen peroxide solution is 5-10% by weight.

[0099] According to the present invention, preferably, the pretreatment conditions include: a temperature of 15-40° C., and a time of 0.5-4 h.

[0100] According to the present invention, preferably, the pretreated fiber cloth can be cleaned under ultrasound and then sterilized.

[0101] According to the present invention, preferably, the cleaning agent used for cleaning is water, preferably double distilled water and / or ultrapure water.

[0102] According to the present invention, preferably, the cells are adherent cells, preferably Vero cells and / or L929 cells.

[0103] In some embodiments, the fiber cloth carrier optionally comprises or is made of multiple layers, preferably 2-7 layers, of the polyester fiber cloth of the present invention. Preferably, weld lines, for example 2-5 weld lines, are located between the multiple layers of polyester fiber cloth. Preferably, the shortest distance between any two weld lines is greater than 0.5 mm. More preferably, the weld lines are straight lines. In the present invention, weld lines refer to lines formed by welding paths, and welding refers to the use of heat to partially fuse multiple layers of polyester fiber cloth.

[0104] In some embodiments, the fiber cloth carrier is preferably circular, elliptical or rectangular. Preferably, the diameter of the circle, the major axis of the ellipse or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm.

[0105] A sixth aspect of the present invention provides a method for preparing the above-mentioned fiber cloth carrier, wherein the method comprises: pretreating and / or sterilizing the polyester fiber cloth.

[0106] Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution.

[0107] Preferably, the sterilization includes at least one of ethylene oxide sterilization, high-temperature steam sterilization and irradiation sterilization, preferably high-temperature steam sterilization.

[0108] In a seventh aspect, the present invention provides use of the composite polyester fiber of the present invention or the composite polyester fiber prepared by the method for preparing the composite polyester fiber of the present invention in preparing a fiber cloth carrier for cell culture.

[0109] An eighth aspect of the present invention provides a use of the polyester fiber cloth or fiber cloth carrier of the present invention in cell culture.

[0110] According to the present invention, the application can preferably increase the cellular sugar metabolism rate. Preferably, using the fiber cloth of the present invention for cell culture can increase the cellular sugar metabolism rate to above 15 g / day, for example, 15-35 g / day, preferably 20-33 g / day, and more preferably 27-32 g / day.

[0111] Preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0112] A ninth aspect of the present invention provides a method for culturing cells, comprising: sterilizing the polyester fiber cloth of the present invention and placing it in a culture medium as a carrier, or placing the fiber cloth carrier of the present invention in a culture medium as a carrier, and inoculating cells on the carrier and culturing in vitro; preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

[0113] The present invention will be described in detail below by way of examples.

[0114] Unless otherwise specified, the spinnerets were from Changzhou Jier Precision Machinery Manufacturing Co., Ltd.

[0115] The Sb content in the core material and the skin material is less than 20 ppm.

[0116] The room temperature is about "15-20°C".

[0117] In the present invention, the melting point is determined by placing approximately 5 mg of a sample in a differential scanning calorimeter (DSC) instrument (TA Instruments Q100). The sample is heated from 20°C to 300°C at a rate of 10°C / min, and the endothermic peak between 100°C and 260°C is observed. The sample exhibits a single endothermic peak within this temperature range. The temperature of the peak is taken as the melting point.

[0118] In the following examples, the homemade polyester involved is prepared by mixing PTA (terephthalic acid, analytical grade), EG (ethylene glycol, analytical grade) and an optional comonomer Y (e.g., 1,4-cyclohexanedimethanol or isophthalic acid, analytical grade) in a reactor according to the required amount of the product, heating to 258-263°C, reacting for about 1 hour, then adding tetrabutyl titanate, raising the temperature to 275-290°C, and vacuuming with an oil pump. The reaction is carried out for about 2 hours to obtain the desired polyester.

[0119] Specifically, the preparation method of homemade polyester A is as follows: 166g PTA and 64g EG are added to a reactor, stirred and heated to 258-263°C, reacted for about 1 hour, then tetrabutyl titanate is added, the temperature is raised to 275-290°C, and vacuum is applied with an oil pump, and the reaction is continued for about 2 hours to obtain the desired polyester. The preparation method of homemade polyester B is as follows: 133g PTA and 51g EG are added to a reactor, stirred and heated to 258-263°C, reacted for about 1 hour, then tetrabutyl titanate is added, the temperature is raised to 275-290°C, and vacuum is applied with an oil pump, and the reaction is continued for about 2 hours to obtain the desired polyester. The preparation method of homemade polyester C is as follows: 133g PTA, 35.4g EG, and 34.6g 1,4-cyclohexanedimethanol are added to a reactor, stirred and heated to 258-263°C, and reacted for about 1 hour. Then, tetrabutyl titanate is added, the temperature is raised to 275-290°C, and vacuum is applied with an oil pump. The reaction is carried out for about 2 hours to obtain the desired polyester.

[0120] Example 1

[0121] (1) Preparation of polyester fiber:

[0122] Homopolyethylene terephthalate (manufactured by Sinopec Yizheng Chemical Fiber Co., Ltd., hereinafter referred to as "Yizheng Chemical Fiber," model FG600, intrinsic viscosity 0.675 dL / g, standard deviation of intrinsic viscosity 0.003 dL / g, melting point 261°C) was used as the core material. Copolyethylene terephthalate (Yizheng Chemical Fiber, model FG702, intrinsic viscosity 0.78 dL / g, standard deviation of intrinsic viscosity 0.006 dL / g, comonomer cyclohexanedimethanol, comonomer content 15 mol%, melting point 140°C) was used as the skin material. The core material to skin material mass ratio was 3, and the fibers were extruded from a spinneret at an outlet temperature of approximately 280°C. The spinneret is equipped with 48 spinneret holes, each shaped like two concentric circles. The inner diameter of the inner circle is 20 microns, with a range (maximum hole diameter minus minimum hole diameter) of 1.2 microns. The outer circle has an inner diameter of 30 microns, with a range of 1.1 microns. After extrusion from the spinneret, the polymer melt is cooled by air at room temperature at a speed of 1.5-1.8 m / s. The cooled fibers are then fed evenly or nearly evenly into five steel circular tubes (with an inner diameter of 2 cm, a distance of 3.5 cm between adjacent tubes, and a length of 1 m). The cooled fibers are then stretched within the tubes at a wind speed of approximately 4000-4500 m / min to produce composite polyester fibers.

[0123] The average diameter of the composite polyester fiber was measured and the standard deviation of the diameter was calculated. The test results are shown in Table 1.

[0124] The average diameter of composite polyester fibers was determined by randomly cutting a small section from each of 50 composite polyester fibers. Scanning electron microscope (SEM) photographs (Hitachi S-4800) were taken. The diameter of each fiber was measured from the photographs to the nearest 0.1 μm. The average of these diameters was calculated using the average method, and the standard deviation of these diameters was calculated using the standard deviation method. The test results are shown in Table 1.

[0125] (2) Preparation of polyester fiber cloth:

[0126] The polyester fibers are laid out using a web laying machine, and then the fiber web of a certain thickness is hot pressed at 190-200°C using a non-embossed polished hot roller to obtain a fiber cloth. The test method for the surface density of the fiber cloth is: use a 1 / 10,000 balance to measure the surface density of 1cm 2 The fiber cloth was weighed and then divided by its area. The thickness of the fiber cloth was measured using a Mitutoyo thickness gauge (model 547-313). The surface density and thickness of the fiber cloth are shown in Table 1.

[0127] Example 2

[0128] The method of Example 1 was followed, except that copolyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol%, melting point 237°C) was used as the skin material.

[0129] Example 3

[0130] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 2, the inner diameter of the inner circle of each spinneret hole of the spinneret used was 18 μm, with a range of 1.1 μm, and the inner diameter of the outer circle was 32 μm, with a range of 1.6 μm.

[0131] Example 4

[0132] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 3.6.

[0133] Example 5

[0134] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 1.6.

[0135] Example 6

[0136] The method of Example 1 was followed, except that the cooled fibers were fed into a slit with a spacing of 1 cm, a width of 50 cm, and a length of 50 cm, and the cooled fibers were stretched at a wind speed of 3500-4000 m / min to obtain polyester fibers.

[0137] Example 7

[0138] The method of Example 6 was followed, except that polyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, standard deviation of intrinsic viscosity 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol% based on all monomers, melting point 237°C) was used as the skin material.

[0139] Example 8

[0140] The method of Example 6 was followed, except that the mass ratio of the core layer material to the skin layer material was 2, the inner diameter of the inner circle of the spinneret's spinneret hole was 18 μm with a range of 1.1 μm, and the inner diameter of the outer circle of the spinneret hole was 32 μm with a range of 1.6 μm.

[0141] Example 9

[0142] The method of Example 6 was followed, except that the mass ratio of the core layer material to the skin layer material was 3.6.

[0143] Example 10

[0144] The method of Example 6 was followed, except that the mass ratio of the core layer material to the skin layer material was 1.6.

[0145] Example 11

[0146] (1) Preparation of polyester fiber:

[0147] Polyester fiber was obtained according to step (1) in Example 1.

[0148] (2) Preparation of polyester fiber cloth:

[0149] The polyester fibers obtained in step (1) are cut to a length of 3±0.1 mm to obtain polyester staple fibers. The polyester staple fibers are uniformly mixed with water and a dispersant to obtain a slurry containing polyester staple fibers, wherein the polyester staple fibers in the slurry contain 15% by weight of polyester staple fibers and 1% by weight of dispersant. The dispersant is polyethylene glycol, fatty alcohol, and polyacrylamide in a weight ratio of 1:1:1, the molecular weight of the polyethylene glycol is 800 g / mol, the fatty alcohol is n-octanol, and the molecular weight of the polyacrylamide is 200,000 g / mol. The slurry containing polyester staple fibers is then transported to the web forming area of ​​a wet web forming machine, water is filtered through a web curtain, and the polyester staple fibers form a fiber web on the web curtain. After the fiber web is dried, it is hot-pressed at 190-200°C using a non-embossed polished hot roller to obtain a fiber cloth.

[0150] Example 12

[0151] The method of Example 11 was followed, except that in step (1), copolyethylene terephthalate (Yizheng Chemical Fiber, model BG804, intrinsic viscosity 0.801 dL / g, intrinsic viscosity standard deviation 0.005 dL / g, comonomer cyclohexanedimethanol, comonomer content 0.75 mol% based on all monomers, melting point 237°C) was used as the skin material.

[0152] Example 13

[0153] The method of Example 11 was followed, except that in step (1), the mass ratio of the core layer material to the skin layer material was 2, the inner diameter of the inner circle of the spinneret's spinneret hole was 18 μm, with a range of 1.1 μm, and the inner diameter of the outer circle of the spinneret hole was 32 μm, with a range of 1.6 μm.

[0154] Example 14

[0155] The method of Example 11 was followed, except that in step (1), the mass ratio of the core layer material to the skin layer material was 3.6.

[0156] Example 15

[0157] The method of Example 11 was followed, except that in step (1), the mass ratio of the core layer material to the skin layer material was 1.6.

[0158] Example 16

[0159] The method of Example 11 is followed, except that in step (2), the polyester fibers obtained in step (1) are cut to a length of 2±0.1 mm to obtain polyester staple fibers. The polyester staple fibers are uniformly mixed with water and a dispersant to obtain a slurry containing polyester staple fibers, wherein the slurry containing polyester staple fibers has a content of 18% by weight of polyester staple fibers and a content of 1.5% by weight of the dispersant. The dispersant is polyethylene glycol, a fatty alcohol, and polyacrylamide in a weight ratio of 1:1.2:1.2, the molecular weight of the polyethylene glycol is 1600 g / mol, the fatty alcohol is n-octanol, and the molecular weight of the polyacrylamide is 250,000 g / mol.

[0160] Example 17

[0161] The method of Example 11 was followed, except that in step (2), the fatty alcohol and polyacrylamide were replaced by polyethylene glycol of equal weight.

[0162] Comparative Example 1

[0163] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 1.

[0164] Comparative Example 2

[0165] The method of Example 1 was followed, except that the mass ratio of the core layer material to the skin layer material was 5.

[0166] Comparative Example 3

[0167] The method of Example 1 was followed, except that the raw material used for homopolyethylene terephthalate was homemade polyester A, which had an intrinsic viscosity of 0.51 dL / g, a standard deviation of the intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.

[0168] Comparative Example 4

[0169] The method of Example 1 was followed, except that the spinneret holes were not arranged in a concentric circle structure, but in a parallel structure that divided the circle into two. The inner diameter of the circular spinneret holes was 30 μm, and the range was 1.1 μm.

[0170] Comparative Example 5

[0171] The method of Example 1 was followed, except that the spinneret had a larger aperture range, with the inner diameter of the inner circle being 20 μm and the range being 3.6 μm, and the inner diameter of the outer circle being 30 μm and the range being 5.3 μm.

[0172] Comparative Example 6

[0173] The method of Example 1 was followed, except that the homopolyethylene terephthalate was replaced by BG85 from Yizheng Chemical Fiber, which had an intrinsic viscosity of 0.879 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0174] Comparative Example 7

[0175] The method of Example 1 was followed, except that the homopolyethylene terephthalate was replaced by a self-made polyester B having an intrinsic viscosity of 0.675 dL / g, a standard deviation of the intrinsic viscosity of 0.031 dL / g, and a melting point of 260°C; the copolyethylene terephthalate was replaced by a self-made polyester C having an intrinsic viscosity of 0.782 dL / g, a standard deviation of the intrinsic viscosity of 0.025 dL / g, and a melting point of 143°C; the comonomer was cyclohexanedimethanol, and the comonomer content was 15 mol%.

[0176] Comparative Example 8

[0177] The method of Example 1 was followed, except that the homopolyethylene terephthalate was replaced by BG804 from Yizheng Chemical Fiber, which had an intrinsic viscosity of 0.801 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 237°C.

[0178] Comparative Example 9

[0179] The method of Example 1 was followed, except that the copolyethylene terephthalate was replaced by BG85 from Yizheng Chemical Fiber, which had an intrinsic viscosity of 0.879 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0180] Comparative Example 10

[0181] The method of Example 1 was followed, except that the wind speed in the circular tube was 1500-2000 m / min.

[0182] Comparative Example 11

[0183] The method of Example 1 was followed, except that after cooling, the molten filaments were not fed into a round tube, but were stretched and laid using a swinging wire method.

[0184] Comparative Examples 12-13

[0185] The method of Example 1 was followed, except that different surface densities and thicknesses of the fiber cloth were obtained by adjusting the process parameters of the laying and hot pressing. The surface density and thickness of the fiber cloth are shown in Table 1.

[0186] Comparative Example 14

[0187] The method of Example 6 was followed, except that the mass ratio of the core layer material to the skin layer material was 1.

[0188] Comparative Example 15

[0189] The method of Example 6 was followed, except that the mass ratio of the core layer material to the skin layer material was 5.

[0190] Comparative Example 16

[0191] The method of Example 6 was followed, except that the raw material used for homopolyethylene terephthalate was homemade polyester A, which had an intrinsic viscosity of 0.51 dL / g, a standard deviation of the intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.

[0192] Comparative Example 17

[0193] The method of Example 6 was followed, except that the spinneret holes were not arranged in a concentric circle structure, but in a parallel structure that divided the circle into two. The inner diameter of the circular spinneret holes was 30 μm, and the range was 1.1 μm.

[0194] Comparative Example 18

[0195] The method of Example 6 was followed, except that the spinneret had a larger aperture range, with the inner diameter of the inner circle being 20 μm and the range being 3.6 μm, and the inner diameter of the outer circle being 30 μm and the range being 5.3 μm.

[0196] Comparative Example 19

[0197] The method of Example 6 was followed, except that the homopolyethylene terephthalate used was BG85 from Yizheng Chemical Fiber, with an intrinsic viscosity of 0.879 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0198] Comparative Example 20

[0199] The method of Example 6 was followed, except that the homopolyethylene terephthalate was replaced by a self-made polyester B having an intrinsic viscosity of 0.675 dL / g, a standard deviation of the intrinsic viscosity of 0.031 dL / g, and a melting point of 260°C; the copolyethylene terephthalate was replaced by a self-made polyester C having an intrinsic viscosity of 0.782 dL / g, a standard deviation of the intrinsic viscosity of 0.025 dL / g, and a melting point of 143°C; the comonomer was cyclohexanedimethanol, and the comonomer content was 15 mol%.

[0200] Comparative Example 21

[0201] The method of Example 6 was followed, except that the homopolyethylene terephthalate was replaced by BG804 from Yizheng Chemical Fiber, which had an intrinsic viscosity of 0.801 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 237°C.

[0202] Comparative Example 22

[0203] The method of Example 6 was followed, except that the co-polyethylene terephthalate was replaced with BG85 from Yizheng Chemical Fiber, which had an intrinsic viscosity of 0.879 dL / g, a standard deviation of the intrinsic viscosity of 0.005 dL / g, and a melting point of 248°C.

[0204] Comparative Example 23

[0205] The method of Example 6 was followed, except that the wind speed in the slit was 1500-2000 m / min.

[0206] Comparative Example 24

[0207] The method of Example 11 was followed, except that the mass ratio of the core layer material to the skin layer material was 1.

[0208] Comparative Example 25

[0209] The method of Example 11 was followed, except that homopolyethylene terephthalate was replaced by self-made polyester A, which had an intrinsic viscosity of 0.51 dL / g, a standard deviation of intrinsic viscosity of 0.01 dL / g, and a melting point of 260°C.

[0210] Comparative Example 26

[0211] The method of Example 11 was followed, except that the spinneret had a larger aperture range, with the inner diameter of the inner circle being 20 μm and the range being 3.6 μm, and the inner diameter of the outer circle being 30 μm and the range being 5.3 μm.

[0212] Comparative Example 27

[0213] The method of Example 11 is followed, except that in step (2), the polyester fiber obtained in step (1) is cut to a length of 10±0.1 mm to obtain polyester staple fibers.

[0214] Comparative Example 28

[0215] The method of Example 1 was followed, except that the speed of the web laying machine receiving the curtain was accelerated to three times the original speed during web laying.

[0216] Test Case

[0217] Pretreatment and sterilization of the fiber cloth and sheet-like fiber cloth carrier: Soak in a 7% by weight hydrogen peroxide solution at room temperature for 60 minutes, then ultrasonically clean with ultrapure water and dry at 80°C. After drying, sterilize the fiber cloth and sheet-like fiber cloth carrier with high-temperature steam at 121°C for 60 minutes.

[0218] Then the sugar metabolism ability of Vero cells was tested when the sterilized fiber cloth or sheet fiber cloth carrier was used as a carrier. The test method for sugar metabolism is as follows: the fiber cloth or sheet fiber cloth carrier was cut into small pieces of 6mm×6mm, 100g of 6mm×6mm fiber cloth or sheet fiber cloth carrier, 8L PBS buffer and about 200mL of 199 culture medium (purchased from Beijing Tsinghua Tianyi Biotechnology Co., Ltd.) were placed in a 10L basket reactor, 10% of high-quality newborn calf serum (purchased from Lanzhou Minhai Bioengineering Co., Ltd.) relative to the culture medium was added, and an appropriate amount of 20% by weight concentration of glucose was added to make the total glucose concentration 0.5% by weight. Vero cells (purchased from the National Biomedical Experimental Cell Resource Bank, 4th generation Vero cells, per 1cm 2 The inoculation volume of cells on the fiber cloth was 0.5×10 6 ), cultured at 37°C with ventilation, pH 7.3, DO (dissolved oxygen as a percentage of saturated dissolved oxygen) 60%, 5% carbon dioxide concentration, and slow stirring during culture for 7 days. Samples were taken every 24 hours and the glucose content was detected using a glucose detection kit (purchased from Nanjing Jiancheng Bioengineering Research Institute), and the glucose concentration was supplemented to 0.5% by weight with a 20% by weight glucose solution. The reduction in the measured glucose content was converted into a glucose consumption rate value in g / day. The glucose consumption rate was plotted against the culture time, and the value at 100 hours was taken as the 100-hour glucose metabolism rate value. The 100-hour glucose metabolism rate values ​​are shown in Table 1.

[0219] Table 1

[0220] The results in Table 1 show that the composite polyester fibers of the present invention or the composite polyester fibers prepared using the method of the present invention have a small standard deviation in diameter. The carrier prepared from the composite polyester fibers of the present invention can significantly improve the sugar metabolism ability of cells.

[0221] The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited thereto. Within the technical concept of the present invention, various simple variations of the technical solution of the present invention may be made, including combining the various technical features in any other appropriate manner. These simple variations and combinations should also be regarded as disclosed in the present invention and fall within the scope of protection of the present invention.

Claims

1. A composite polyester fiber, characterized in that: The composite polyester fiber has a skin-core structure; wherein the mass ratio of the core layer material to the skin layer material is x, and 1.5≤x≤4, preferably 2≤x≤3.6, and more preferably 2≤x≤3; and The average diameter D of the composite polyester fiber is 10-40 μm, preferably 17-32 μm, more preferably 18-30 μm, and the standard deviation of the diameter is ≤2.2 μm, preferably ≤2.0 μm, more preferably ≤1.8 μm.

2. The composite polyester fiber according to claim 1, wherein The melting point of the core material is higher than that of the skin material by more than 20°C, preferably more than 22°C; the intrinsic viscosity of the skin material is higher than that of the core material by 0.01-0.18 dL / g, preferably 0.01-0.15 dL / g, and the standard deviation of the intrinsic viscosity of the core material and the skin material is ≤0.02 dL / g, preferably ≤0.015 dL / g; and / or The core layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably homopolyethylene terephthalate, and the skin layer material is homopolyethylene terephthalate, copolyethylene terephthalate or a combination thereof, preferably copolyethylene terephthalate.

3. The composite polyester fiber according to any one of claims 1 to 2, characterized in that The mass ratio x of the core layer material to the skin layer material of the composite polyester fiber and the average diameter D of the composite polyester fiber satisfy the following formula (1): The numerical value of the average diameter D of the composite polyester fiber used in formula (1) is in micrometers.

4. The composite polyester fiber according to any one of claims 1 to 3, wherein The core layer material is homopolyethylene terephthalate; preferably, the intrinsic viscosity of the homopolyethylene terephthalate is 0.6-0.8dL / g, preferably 0.62-0.8dL / g, more preferably 0.65-0.7dL / g, and the melting point is above 240°C, more preferably 245-280°C, and / or preferably, the standard deviation of the intrinsic viscosity of the homopolyethylene terephthalate is ≤0.015dL / g, preferably ≤0.01dL / g, more preferably ≤0.005dL / g.

5. The composite polyester fiber according to any one of claims 1 to 4, in, The skin material is copolyethylene terephthalate; Preferably, the intrinsic viscosity of the co-polyethylene terephthalate is 0.601-0.9 dL / g, preferably 0.61-0.9 dL / g, more preferably 0.61-0.82 dL / g, and the melting point is below 240°C, more preferably 130-240°C, and / or Preferably, the standard deviation of the intrinsic viscosity of the co-polyethylene terephthalate is ≤0.015 dL / g, preferably ≤0.01 dL / g.

6. The composite polyester fiber according to claim 5, wherein The copolyethylene terephthalate comprises structural units derived from terephthalic acid, ethylene glycol and comonomer Y, and the content of the structural units derived from comonomer Y in the copolyethylene terephthalate is 0.6-20 mol %, preferably 0.6-18 mol %, and more preferably 0.7-15 mol %; Preferably, the comonomer Y is selected from dicarboxylic acids, diols and tetraols, preferably at least one selected from isophthalic acid, phthalic acid, butanediol, hexanediol, cyclohexanedimethanol and pentanediol.

7. A method for preparing the composite polyester fiber according to any one of claims 1 to 6, characterized in that: The method comprises: melting the core layer material and the skin layer material respectively to obtain a melt, and spinning the melt to obtain the composite polyester fiber.

8. The method for preparing a composite polyester fiber according to claim 7, in, The shape of each spinneret of the spinneret used in the spinning process is two concentric circles, the difference between the maximum diameter and the minimum diameter of all inner circles of the spinneret is ≤ 2 μm, and the difference between the maximum diameter and the minimum diameter of all outer circles of the spinneret is ≤ 2 μm, and / or The inner diameter of the inner circle of the spinneret hole is 15-25 μm, and the inner diameter of the outer circle is 25-35 μm.

9. The method according to any one of claims 7 to 8, wherein the method comprises: (1) feeding the melted core layer material and the skin layer material into a spinneret for spinning to obtain a filamentary melt having a skin-core structure; (2) cooling the filament melt having a skin-core structure to obtain a primary fiber; and (3) The spun fibers are stretched to obtain the composite polyester fibers.

10. The method according to claim 9, wherein: The spun fibers are stretched using a high-speed airflow, preferably a high-speed airflow of 2500-5500 m / min; preferably, the stretching includes at least one of a circular tube stretching method and a slit stretching method.

11. The method according to claim 10, wherein: In the slit stretching method, the slits have a spacing of 0.3-3 cm, a width of 10-60 cm and a length of 10-60 cm; or In the round tube method, the inner diameter of the round tube is 0.5-5 cm, the distance between two adjacent round tubes is 0.5-10 cm, and the length of the round tube is 0.1-2 m.

12. A polyester fiber cloth, characterized in that: The polyester fiber cloth comprises the composite polyester fiber according to any one of claims 1 to 6 or the composite polyester fiber produced by the method according to any one of claims 7 to 11.

13. The polyester fiber cloth according to claim 12, wherein The polyester fiber cloth has a thickness of 0.35-0.6 mm, preferably 0.4-0.5 mm, and a surface density of 70-160 g / m 2 , preferably 80-130g / m 2 ; and / or The polyester fiber cloth is a non-woven fabric; and / or The standard deviation of fiber diameters in the polyester fiber cloth is ≤2.4 μm, preferably ≤2.2 μm, and more preferably ≤2.0 μm.

14. A method for preparing the polyester fiber cloth according to any one of claims 12 to 13, comprising laying and hot pressing the composite polyester fiber to obtain the polyester fiber cloth.

15. The method according to claim 14, wherein: The temperature of the hot pressing is 180-220°C.

16. The method according to claim 14 or 15, wherein: The slurry containing the short fibers of the composite polyester fibers is wet-laid.

17. The method according to claim 16, wherein: The content of the composite polyester fiber in the slurry is 5-30% by weight, preferably 10-20% by weight, and / or The slurry further comprises a dispersant, preferably, the dispersant is at least one of polyethylene glycol, fatty alcohol and polyacrylamide, and / or preferably, the content of the dispersant in the slurry is 0.05-2% by weight.

18. The method according to claim 17, wherein: The dispersant is a combination of polyethylene glycol, fatty alcohol and polyacrylamide, wherein the weight ratio of the polyethylene glycol, fatty alcohol and polyacrylamide is 1:0.5-2:0.5-2; More preferably, the molecular weight of the polyethylene glycol is 400-4000 g / mol; more preferably, the fatty alcohol is a fatty alcohol having 3-12 carbon atoms, and further preferably, the number of hydroxyl groups in the fatty alcohol is 1-3; and / or more preferably, the molecular weight of the polyacrylamide is 50000-5000000 g / mol.

19. A fiber cloth carrier for cell culture, characterized in that: The fiber cloth carrier is made of the polyester fiber cloth according to any one of claims 12 to 13 or is prepared by the method for preparing the polyester fiber cloth according to any one of claims 14 to 18.

20. The fiber cloth carrier according to claim 19, wherein: The fiber cloth carrier is prepared by pretreating and / or sterilizing the polyester fiber cloth; Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution; and / or Preferably, the sterilization includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

21. The fiber cloth carrier according to any one of claims 19 to 20, characterized in that It comprises multiple layers, preferably 2-7 layers, of polyester fiber cloth according to any one of claims 12 to 13; preferably, there are welding lines between the polyester fiber cloths, preferably the shortest distance between any two welding lines is >0.5 mm, and more preferably, the welding lines are straight lines.

22. The fiber cloth carrier according to any one of claims 19 to 21, characterized in that The fiber cloth carrier is circular, elliptical or rectangular; preferably, the diameter of the circle, the major axis of the ellipse or the length of the rectangle is greater than or equal to 2 mm and less than or equal to 15 mm.

23. A method for preparing a fiber cloth carrier according to any one of claims 19 to 22, characterized in that: The method comprises: pre-treating and / or sterilizing the polyester fiber cloth; Preferably, the pretreatment comprises soaking the polyester fiber cloth in a hydrogen peroxide solution; and / or Preferably, the sterilization includes at least one of ethylene oxide sterilization, high temperature steam sterilization and irradiation sterilization, preferably high temperature steam sterilization.

24. Use of the composite polyester fiber according to any one of claims 1 to 6 or the composite polyester fiber prepared by the method according to any one of claims 7 to 11 in preparing a fiber cloth carrier for cell culture.

25. Use of the polyester fiber cloth according to any one of claims 12 to 13 or the fiber cloth carrier according to any one of claims 19 to 22 in cell culture, preferably the cells are adherent cells, more preferably Vero cells and / or L929 cells.

26. A method for culturing cells, characterized in that: The method comprises: sterilizing the polyester fiber cloth according to any one of claims 12 to 13 and placing it in a culture medium as a cell carrier, or placing the fiber cloth carrier according to any one of claims 19 to 22 in a culture medium as a cell carrier, and Cells are inoculated on the cell carrier and cultured in vitro; preferably, the cells are adherent cells, more preferably Vero cells and / or L929 cells.

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