Systems and methods for capturing and processing of images

Abstract

The present invention relates to systems and methods for image capturing and processing. In an example, a microscopy imaging system (222) is 5 described, which includes a compact optical microscopy device (102) having an optical lens assembly (104) with eight to sixteen lens elements and an image sensor (106) to capture an image of an object through the optical lens assembly (104). The microscopy imaging system (222) further includes a processing unit (228) communicatively coupled to the compact 10 optical microscopy device (102) for the image processing.

Description

SYSTEMS AND METHODS FOR CAPTURING AND PROCESSING OF IMAGESTECHNICAL FIELD

[0001] The present subject matter relates to microscopy imaging system for capturing and processing images of objects. More specifically, the present subject matter relates to a microscopy imaging system for automated detection, quantification and analysis of objects and further quick interpretation of various sectors.BACKGROUND

[0002] The smartphone and other portable computing devices can be considered as the pinnacle of consumer technology of the modern Information Age. A standard smartphone is a portable computer with a digital camera, a high-resolution display, a range of remote sensors, an audio-visual interfacing, and an internet access and interconnectivity. This is all combined in an accessible package with constantly evolving software and associated applications.

[0003] Microscopes are generally used to view objects that are too small to be seen by the unaided eye. Optical microscopes use visible light and an assembly of optical lenses to magnify images of small objects. Optical microscopes are used in observing small structures, determining pathology, and diagnosing diseases.BRIEF DESCRIPTION OF THE DRAWINGS

[0004] The foregoing summary, as well as the following detailed description of the present subject matter, is better understood when read in conjunction with the appended drawings. For the purpose of illustrating the present subject matter, exemplary constructions of the present subject matter are shown in the drawings. However, the present subject matter is not limitedto the specific process and structures disclosed herein. The description of a process step or a structure referenced by a numeral in a drawing is applicable to the description of that method step or structure shown by that same numeral in any subsequent drawing herein.

[0005] FIG. 1 A shows a block diagram of a portable imaging system, in accordance with the present subject matter;

[0006] FIG. 1 B shows a portable imaging systems with two compact optical microscopy devices, in accordance with the present subject matter;

[0007] FIG. 1 C shows a portable imaging system with one compact optical microscopy device, in accordance with the present subject matter;

[0008] FIG. 2 illustrates a system environment for capturing and processing an image, in accordance with an example of the present subject matter;

[0009] FIG. 3A illustrates a compact optical microscopy device, in accordance with an example of the present subject matter;

[0010] FIG. 3B illustrates a compact optical microscopy device, in accordance with another example of the present subject matter;

[0011] FIG. 3C illustrates an optical microscopy device, in accordance with another example of the present subject matter;

[0012] FIG. 3D illustrates a compact optical microscopy device, in accordance with yet another example of the present subject matter;

[0013] FIG. 3E illustrates a compact optical microscopy device, in accordance with yet another example of the present subject matter;

[0014] FIG. 3F illustrates a compact optical microscopy device, in accordance with yet another example of the present subject matter;

[0015] FIG. 4A illustrates an external light source arrangement with diffusor or polarizer, in accordance with an example of the present subject matter;

[0016] FIG. 4B illustrates an external light source arrangement with diffusor or polarizer, in accordance with another example of the present subject matter;

[0017] FIG. 4C illustrates an external light source arrangement with diffusor or polarizer, in accordance with yet another example of the present subject matter;

[0018] FIG. 5A illustrates an external Light source arrangement without diffusor, in accordance with an example of the present subject matter;

[0019] FIG. 5B illustrates an external Light source arrangement without diffusor, in accordance with another example of the present subject matter;

[0020] FIG. 6 illustrates an external Light source arrangement for dark field and filter field microscopy, in accordance with an example of the present subject matter;

[0021] FIG. 7A to Fig. 15C represents example layouts of compact optical microscopy devices, and associated distortion plots and modulation transfer function (MTF) plots, in accordance with the present subject matter;

[0022] FIG. 16 illustrates a flow chart depicting a method for processing an image of an object, in accordance with an example implementation of the present subject matter; and

[0023] FIG. 17 illustrates an external light source and sample holder arrangement, in accordance with an example of the present subject matter.DETAILED DESCRIPTION

[0024] Optical microscopes are commonly used in various research areas including microbiology, microelectronics, nanophysics, biotechnology, and pharmaceutical research. Optical microscopes use visible light and an assembly of optical lenses to magnify images of small objects. However, such optical microscopes are not portable.

[0025] Therefore, conventional portable microscopy assemblies came into existence. The conventional portable microscopy assemblies are available in the form of external optical attachments to the smartphones or other portable computing devices. But it has several limitations. The external optical attachments for the microscopy functionality have limitations as theyhave limited field of view, are bulky, are not compatible for all digital device, and are not user-friendly for focusing and microscopy. The microscopy performance of the conventional optical attachments varies with respect to different models of smartphones with different quality of cameras. The external optical attachments for smartphones are not user-friendly because the location of the camera varies across smartphones.

[0026] Furthermore, most of the external optical attachments for smartphones have a fixed optical system; hence, it has limitation for focusing of objects of variable sizes on the same plane. The external optical attachment for the microscopic functionality makes it inaccessible to users for qualitative & quantitative estimation of objects.

[0027] Furthermore, some of the objects has ability to emit light rays of specific wavelengths that are characteristic of the respective objects and are therefore useful for qualitative and quantitative analysis of such objects. This said characteristic feature of light is useful in the diagnosis of many pathological samples, for example, a biomarker-dye complex. Most of the external optical attachments for portable microscopy are not affordable.

[0028] Further, conventional optical attachments have a limited resolution for the observing of the micron size of object and the two or three correction lenses. Such attachments are not able to fulfill the high-quality image capturing of objects of micron size.

[0029] In addition, the image captured by the conventional optical devices is processed in a manner that the processed image can be applied for the interpretation of the various sectors. However, due to the lack of high-quality image capturing of objects of micron size, the processed image is not accurate to a level that can be precisely applied for the interpretation of the various sectors.

[0030] Therefore, to overcome the problems / limitations said in the prior art, there is a need to develop microscopy imaging systems having optical microscopy devices for enabling the inbuilt microscopy feature in smartphones and other portable imaging systems and having smart imageprocessing capabilities. In addition to the inbuilt microscopy feature, the features of variable imaging resolution, variable depth of field, variable optical magnification, and wide field of view, but without optical and spherical aberrations are also desirable to be enabled. Furthermore, the inbuilt microscopy feature with the variable optical magnification, and the variable depth of field facilitate in increasing the flexibility to view a wide range of objects using the smartphones and the portable imaging systems. The smartphones and other portable imaging systems with the integrated microscopy feature makes them accessible to users, such as researchers, pupils, and professionals for education purposes, onsite diagnosis, and in qualitative and quantitative analysis of objects.

[0031] Detailed embodiments of the present subject matter are disclosed herein, however, it is to be understood that the disclosed embodiments are merely exemplary of the present subject matter, which may be embodied in various forms. Therefore, specific functional and structural details disclosed herein are not to be interpreted as limiting, but merely as a basis for the claims and as a representative basis for teaching one skilled in the art to variously employ the present subject matter in virtually any appropriately detailed structure.

[0032] The present subject matter overcomes the aforesaid drawbacks of conventional devices. The objects, features, and advantages of the present subject matter will now be described in greater detail. Also, the following description includes various specific details and is to be regarded as merely exemplary. Accordingly, those of ordinary skill in the art will recognize that: without departing from the scope and spirit of the present disclosure and its various embodiments there may be any number of changes and modifications described herein.

[0033] It must also be noted that as used herein and in the appended claims, the singular forms "a", "an," and "the" include plural references unless the context clearly dictates otherwise. Although any systems and methods similar or equivalent to those described herein can be used in the practiceor testing of embodiments of the present subject matter, the preferred, systems are now described.

[0034] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present subject matter belongs.

[0035] The word “system” and “device” may be used interchangeably through the disclosure. In addition to the features and components described herein, the systems or the devices may include one or more other components, such as a process, a memory, an interface, a display screen, an image capturing unit, a graphical user interface.

[0036] Throughout the disclosure, term ‘assembly’ or ‘smartphone’ or ‘smartphone with integrated microscopy features’ used herein refers to an instrument that contain a built-in camera lens assembly, a camera, and a display screen to produce a simple microscope platform; a digital image capturing assembly; or a device for qualitative or quantitative estimation. The terms ‘assembly’, ‘smartphone’ and ‘smartphone with integrated microscopy features’ can be used interchangeably throughout the specification.

[0037] Throughout the disclosure, term ‘other device’ or ‘other device with integrated microscopy features’ used herein refers to an image capturing assembly, a digital portable device for image capturing or any of digital device for observation of object. The terms ‘other device’ and ‘other device with integrated microscopy features’ can be used interchangeably throughout the specification.

[0038] Systems and methods of the present subject matter approaches for automated detection, quantification and analysis of objects and further quick interpretation of various sectors such as healthcare, water, food, education, research, microbiology, biotechnology, botany, and agriculture etc. In an example, a microscopy imaging system includes an inbuilt multi-mode microscopy feature of the smartphone with the light guiding element and digital devices in combination with an image processing on cloud orstandalone software. Some of the applications, but is not limited to, of the microscopy imaging system of the present subject matter are in food and water contamination detection, adulteration detection in herb or plant products, size distribution analysis, total cell counting, cell viability testing, automated object detection in the education sector and interactive learning, microbial growth and contamination detection in bioreactors, healthcare diagnosis. The inbuilt multi-mode microscopy such as light field, dark field, and filter field microscopy with an integration of digital database and artificial intelligence and machine learning based image processing provides an onsite, quick, and rapid analysis of an object or a sample.

[0039] In an example implementation, the microscopy imaging system may include a compact optical microscopy device and a processing unit communicatively coupled to the compact optical microscopy device. The compact optical microscopy device provides integrated microscopy in a portable imaging system, such as a smartphone and other devices. The integrated microscopy features include variable depth of field, resolution, field of view, and magnification. The integrated microscopy features can be used for the quantitative and qualitative analysis of micron-size objects that emit the light. The term “micron” herein may be understood as micrometer (pm).

[0040] The compact optical microscopy device allows for inbuilt light field, near field, and filter field microscopy with variable optical magnification, resolution, field of view, and high depth of field microscopy functionality. The compact optical microscopy device of the present subject matter is used in a portable imaging system. The portable imaging system may include, but is not limited to, a smartphone, a mobile phone, a portable computing device, a portable medical device, a portable microscope, or a portable analytical instrument. The portable computing device may include, but is not limited to, a laptop, a netbook, a notebook, a sub-notebook, a tablet computing device, and an Ultrabook computer. The portable medical device may include, but is not limited to, a small pocket size medical device, ananalytical instrument, a diagnosis device, and a quantitative and qualitative analytical instrument. The portable microscope may include, but is not limited to, a pocket size portable microscope and a portable digital microscope device.

[0041] The compact optical microscopy device includes multiple elements, which are sequentially arranged from the object side to the image side for the microscopy functionality in portable imaging systems, such as the smartphones and / or other devices.

[0042] In an example of the present subject matter, the compact optical microscopy device includes an optical lens assembly with eight to sixteen lens elements out of which five lens elements are an objective lens group. The five lens elements of the objective lens group comprise two correction lenses. The multiple lens element in the optical microscopy device provide microscopy features such as variable optical magnification, resolution, field of view, and high depth of field. The optical lens assembly has an optical magnification in a range of about 1 X to about 7.8X, an airy radius in a range of about 0.783 micron to about 23.25 micron, a depth of field in a range of about 2.847 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.438, a half field of view in a range of about 13 degrees to about 70 degrees, and a length in a range of about 5.51 1 millimeter (mm) to about 57 mm. The compact optical microscopy device further includes an image sensor to capture an image of an object through the optical lens assembly. The image sensor may have a pixel pitch in a range of about 0.2 micron to about 6 micron. The image sensor has a wavelength-specific filter or IR filter for light field and filter field microscopy.

[0043] Further, the processing unit of the microscopy imaging system may process the captured image by converting the image to a digitized form. In an example, processing the captured image may include resizing the image to a specific resolution, normalizing pixel values of the image, and augmenting the image data. Common data augmentation techniques include random rotation, flipping, and brightness adjustments. Theprocessing unit may further detect a unique or multiple characteristic feature of the object by analyzing the digitized form of the image using an artificial intelligence and machine learning based model. By processing the captured images, the images in the training set are consistently formatted, normalized to a common range, and augmented to increase the diversity of the data. The pre-processed images thus help the artificial intelligence and machine learning based model to focus on characteristic features during training and improve robustness of the model to variations in the input data.

[0044] The detected characteristic feature may be compared with pre-stored dataset. In an example implementation, the artificial intelligence and machine learning based model may detect the unique or multiple characteristic feature of the object using a common technique called Local Binary Patterns (LBP). LBP is a texture descriptor that represents the patterns formed by comparing an intensity of a center pixel with an intensity of surrounding neighbor pixel. The LBP assigns a binary code to each pixel in an image based on whether an intensity of a neighboring pixel is greater or less than an intensity of the center pixel.

[0045] For example, given an image patch with pixel intensities represented as l(x, y), where (x, y) are the pixel coordinates, and N(i, j) is a set of neighboring pixels, the LBP value at the center pixel (x, y) is computed as follows:LBP(x, y) = Z [s(l(i, j) - l(x, y)) « p] for (i, j) e N(x, y)

[0046] Here, s() is the sign function, and « denotes bitwise left shift, p is a threshold, and the LBP value is a binary number represented in decimal notation.

[0047] In another example implementation, the artificial intelligence and machine learning based model may detect the unique or multiple characteristic feature of the object using deep learning feature extraction performed by convolutional neural networks (CNNs). During the training process, the CNNs learn to automatically extract unique or multiplecharacteristics features from raw images. The CNNs involve convolutional operations and non-linear activation functions. The convolutional operations are performed by convolutional layers in CNNs. The convolutional layers may apply filters (kernels) to the input image to extract the unique or multiple characteristic features. The convolution operation can be mathematically represented as follows:C(x, y) = Z [l(i, j) * K(x-i, y-j)] for (i, j) e N(x, y)

[0048] Here, l(i, j) is the pixel intensity at location (i, j) in the input image, K(x-i, y-j) is the value of the convolutional filter at relative coordinates (x-i, y-j), and N(x, y) is the set of neighboring pixels around position (x, y).

[0049] After the convolution operation, a non-linear activation function (e.g., ReLU - Rectified Linear Unit) is applied element-wise to the convolutional feature maps to introduce non-linearity into the model.ReLU(x) = max(0, x)

[0050] This allows the network to learn complex and nonlinear relationships between the unique or multiple characteristic features. Deep learning-based feature extraction eliminates the need for hand-crafting features, making the image processing more flexible and capable of learning intricate patterns directly from the data.

[0051] For the comparison, the detected characteristic feature is mapped with a relevant parameter, of the object, present in the pre-stored dataset. The unique or multiple characteristic feature may be a feature, for example white blood cells in a blood sample if the object is the blood sample. In an example implementation, Convolutional Neural Networks (CNNs) may be used for mapping detected characteristic feature with a relevant parameter due to their ability to learn hierarchical features from images. Depending on the complexity of the task and the size of the dataset, different CNN architectures such as VGG, ResNet, Inception, or more recent ones like EfficientNet or DenseNet may be selected.

[0052] Based on the comparison, a report may be generated to quantify a category of the detected characteristic feature of the object. In an example implementation, the artificial intelligence and machine learning based model may quantify a category of the detected characteristic feature of the object using a state-of-the-art CNN architecture, i.e., EfficientNet that achieves impressive performance across various image classification tasks while being computationally efficient. The report is a presentable format indicative of the category to which the unique or multiple characteristic feature of the object belongs after the mapping. For example, in case of a blood sample, if the unique or multiple characteristic feature of the object after the mapping is indicative of a bacteria type, then the report may quantify the category as bacterial fever.

[0053] In an example, the compact optical microscopy device may include a focusing unit with focusing mechanism for controlled focusing of object at various depth of fields. The focusing unit has a barrel, where the optical lens assembly of the compact optical microscopy device is disposed within the barrel of the focusing unit. The focusing unit is to operate the optical lens assembly for focusing while imaging the object.

[0054] In an example, the optical lens assembly may include a first lens element configured as a wavelength-specific optical filter.

[0055] In an example, the optical lens assembly may include a last lens element configured as a wavelength-specific optical filter.

[0056] In an example, the compact optical microscopy device may include a wavelength-specific optical filter positioned between a first lens element and a second lens element of the optical lens assembly.

[0057] In an example, the compact optical microscopy device may include a wavelength-specific optical filter positioned in front of the first lens element.

[0058] In an example, the compact optical microscopy device may include a wavelength-specific optical filter positioned between the image sensor and a last lens element of the optical lens assembly.

[0059] In an example, the compact optical microscopy device may include a wavelength-specific optical filter positioned between an adjacent pair of lens elements of the optical lens assembly.

[0060] In an example, the compact optical microscopy device may include a first light source. The first light source may include at least one light emitting diode (LED) placed at a circumferential boundary of the optical lens assembly to emit light such that the emitted light is incident from a first side on a sample held within a sample holder unit, when the sample holder unit is placed against the optical lens assembly.

[0061] In an example, the first light source comprises a plurality of LED placed at a circumferential boundary of the optical lens assembly to emit light in a form of a ring such that the emitted light is incident on a sample held within a sample holder unit, when the sample holder unit is placed over the optical lens assembly. In an example, the LED is a white LED. In an example, the LED is a wavelength-specific LED emitting light of a wavelength in a range of about 200 nm to about 700 nm.

[0062] In an example, the LED may be placed such that the emitted light is incident on the sample in the sample holder unit at an angle in a range of about 5° to about 90° with respect to a planar surface of the sample holder unit. In an example, the LED may be placed such that the emitted light is incident on the sample in the sample holder unit at an angle in a range of about 48° to about 49° with respect to a planar surface of the sample holder unit. In an example, the LED may be placed such that the emitted light is incident on the sample in the sample holder unit at an angle of about 48.5° with respect to a planar surface of the sample holder unit.

[0063] In an example, the compact optical microscopy device may include a polarized filter disposed in front of a first lens element of the optical lens assembly.

[0064] In an example, the compact optical microscopy device may include a protective glass cover disposed in front of a first lens element of the optical lens assembly.

[0065] In an example, the compact optical microscopy device may include a sample holding unit. The sample holding unit includes at least one compartment to hold a sample to be imaged by the optical lens assembly. In an example, the sample holding unit may be magnetically coupled to a housing of the compact microscopy imaging device for imaging the sample by the optical lens assembly. In an example, the sample holding unit may be movable at least in one direction in a plane in which the housing extends for imaging the sample by the optical lens assembly.

[0066] In an example, the sample holding unit may include a planar surface having a wavelength-specific filter. In another example, the sample holding unit may include a top cover to cover the compartment. The top cover is a wavelength-specific filter.

[0067] In an example, the sample holding unit may include a sample injection port corresponding to each compartment. In an example, the compact microscopy imaging device may include a first wavelength-specific filter disposed on a first planar side of the compartment and a second wavelength-specific filter disposed on a second planar side of the compartment. The second planar side is opposite to the first planar side. In an example, the compartment may include an inlet for inflow of the sample and an outlet for outflow of the sample.

[0068] In an example, the compact microscopy imaging device may include a second light source to provide light from a second side to the sample in the sample holding unit. The second side is opposite the first side. The second light source may be movable relative to the optical lens assembly to vary a distance between a planar surface of the sample holding unit. In an example the second light source is a point light source or a diffuse light source or a combination thereof and is detachably mounted in a housing of the second light source.

[0069] In an example, the second light source may include one of variable intensity of light, variable wavelength of light, and variable area of lightexposure on the sample in the sample holding unit. In an example, the second light source is a white LED.

[0070] In an example, the second light source may include a housing and a cavity with electrical contacts to mount a white LED or a wavelength-specific LED.

[0071] In an example, the compact microscopy imaging device may include a diffuser or a polarized filter between the second light source and the sample holding unit.

[0072] In an example, the compact microscopy imaging device may include a convex lens between the second light source and the sample holding unit.

[0073] In an example, the second light source may include a white LED, and the compact microscopy imaging device may include a convex lens and a ring shaped aperture. The convex lens is placed to direct the light, from the white LED, passing through the ring shaped aperture onto the sample in the sample holder unit.

[0074] In an example, the second light source may include a wavelengthspecific LED emitting light of a wavelength in a range of about 200 nm to about 700 nm, and wherein the microscopy apparatus comprises a convex lens and a ring shaped aperture, wherein the convex lens is placed to direct the light, from the white LED, passing through the ring shaped aperture onto the sample in the sample holder unit.

[0075] In an example, the second light source may include a housing with a reflective surface and two LEDs mounted on opposite surfaces to reflect light onto the sample in the sample holding unit.

[0076] The present subject matter enables the ability to see the object from macron size to micron size. The advancement of smartphones and other devices with the high-resolution microscopic feature and image processing capabilities can be used for the automated qualitative and quantitative estimation of a wide range of objects for various applications such as but not limited to diagnosis, analytical applications, research and education applications, portable devices, image capturing and analytical devices etc.The microscopy imaging system of the present invention may scale up or scale down to provide microscopic features in small package sizes. The size of the overall lens assembly is more compact with prevention of the optical and spherical aberrations; hence, it can fit in to the smartphone, any of digital image capturing device, portable device for qualitative and quantitative analysis of micron size of object or object that is not visible to the naked eyes.

[0077] The present subject matter enables to provide inbuilt multi-mode microscopic features such as light field, dark field, and filter field microscopy with automated detection, quantification, and qualitative analysis of the sample for quick and rapid detection and analysis of samples. In addition, inbuilt microscopic features such as variable depth of field, variable optical magnification, and variable resolution without any optical and spherical aberrations are also enabled with the implementations of the present subject matter.

[0078] The present subject matter is further described with reference to the accompanying figures. Wherever possible, the same reference numerals are used in the figures and the following description to refer to the same or similar parts. It should be noted that the description and figures merely illustrate principles of the present subject matter. It is thus understood that various arrangements may be devised that, although not explicitly described or shown herein, encompass the principles of the present subject matter. Moreover, all statements herein reciting principles, aspects, and examples of the present subject matter, as well as specific examples thereof, are intended to encompass equivalents thereof.

[0079] Referring to figures, FIG. 1 A shows a portable imaging system 100 having a compact optical microscopy device 102. The compact optical microscopy device 102 may include an optical lens assembly 104 and an image sensor 106. The optical lens assembly 104 may include eight to sixteen lens elements. The optical lens assembly 104 has an optical magnification in a range of about 1 X to about 7.8X, an airy radius in a rangeof about 0.783 micron to about 23.25 micron, a depth of field in a range of about 2.847 micron to about 338 micron, a numerical aperture in a range of about 0.015 to about 0.438, a half field of view in a range of about 13 degrees to about 70 degrees, and a length in a range of about 5.511 millimeter (mm) to about 57 mm. The image sensor 106 is disposed to capture an image of an object through the optical lens assembly 104. In an example, the image sensor 106 has a pixel pitch in a range of about 0.2 micron to about 6 micron.

[0080] In an example, the portable imaging system 100 may include two compact optical microscopy device 102, in accordance with an example implementation shown in FIG. 1 B. FIG. 1 C shows a portable imaging system 100 with one compact optical microscopy device 102, in accordance with an example implementation. The portable imaging system 100 may include, but is not limited to, a smartphone, a mobile phone, a portable computing device, a portable medical device, a portable microscope, or a portable analytical instrument. The portable computing device may include, but is not limited to, a laptop, a netbook, a notebook, a sub-notebook, a tablet computing device, and an Ultrabook computer. The portable medical device may include, but is not limited to, a small pocket size medical device, an analytical instrument, a diagnosis device, and a quantitative and qualitative analytical instrument. The portable microscope may include, but is not limited to, a pocket size portable microscope and a portable digital microscopy device.

[0081] Although FIG. 1 B and FIG. 1 C show two compact optical microscopy devices and one compact optical microscopy device, respectively, in the portable imaging system 100, in an example, the portable imaging system 100 may include any number of compact optical microscopy devices. In an example, the portable imaging system 100 may include one to six compact optical microscopy devices.

[0082] Each compact optical microscopy device 102 is embedded inside the portable imaging system 100, for example, while manufacturing the portableimaging system 100. The compact optical microscopy device 102 may replace the existing camera lens assembly within the portable imaging system 100 or may be embedded as an additional unit for enabling microscopy feature in the portable imaging system 100.

[0083] In an example, each compact optical microscopy device 102 may include a light source 108 arranged in periphery of the optical microscopy device 102 as shown in FIGS. 1 B and 1 C. In another example, the light source 108 may be external to the compact optical microscopy device 102. The light source 108 may be a white light source or a light source of specific and desirable wavelength.

[0084] FIG. 2 illustrates a network environment 220 for the automated detection, quantification and analysis of objects, according to an example. The network environment 220 includes a microscopy imaging system 222 for used for the automated detection, quantification and analysis of objects, according to an example. Examples of the microscopy imaging system 222 may include, but are not limited to, a laptop, a notebook computer, a server computer, a tablet computer, and a smartphone. The microscopy imaging system 222 may be used to capture and process the image of the sample related to fields including one of adulteration detection, agriculture, cell viability counting, education, food contamination, healthcare, microbial growth rate, size distribution analysis, and water contamination. In an example, the portable imaging system 100 may be connected to a database 224 through a network 226. The database 224 may be, for example, a structured query language (SQL) data store or a not only SQL (NoSQL) data store. In an exemplary implementation, the database 224 may be configured as cloud-based database. In another exemplary implementation, the database 224 may be a location on a file system directly accessible by the engines. The database 224 may be configured to store cell viability data, food adulteration data and the like.

[0085] The network 226 may be a wireless network, a wired network, or a combination thereof. The network 226 can also be an individual network ora collection of many such individual networks, interconnected with each other and functioning as a single large network, e.g., the Internet or an intranet. The network 226 can be implemented as one of the different types of networks, such as intranet, local area network (LAN), wide area network (WAN), the internet, and such. The network 226 may either be a dedicated network or a shared network, which represents an association of the different types of networks that use a variety of protocols, for example, Hypertext Transfer Protocol (HTTP), Transmission Control Protocol / lnternet Protocol (TCP / IP), Wireless Application Protocol (WAP), etc., to communicate with each other.

[0086] In one implementation, the network environment 220 may be a lab network, including thousands of office personal computers, laptops, various servers, such as blade servers, and other computing devices connected over the network 226.

[0087] The microscopy imaging system 222 may include a processing unit 228. The processing unit 228 may include microprocessors, microcomputers, microcontrollers, digital signal processors, central processing units, state machines, logic circuitries, and / or any other devices that manipulate signals and data based on computer-readable instructions. Further, functions of the various elements shown in the figures, including any functional blocks labelled as “processor(s)”, may be provided through the use of dedicated hardware as well as hardware capable of executing computer-readable instructions. In one example, the microscopy imaging system 222 may be a standalone server or may be a remote server on a cloud computing platform. In a preferred example, the microscopy imaging system 222 may be a cloud-based system. The microscopy imaging system 222 is capable of delivering applications (such as cloud applications) for image processing. In an example, the processing unit 228 may be disposed within the compact optical microscopy device 102. In an example, the processing unit 228 may be disposed outside the compact optical microscopy device 102.

[0088] Further, the microscopy imaging system 222 includes interface(s) 230 and memory(s) 232. The interface(s) 230 may allow the connection or coupling of the microscopy imaging system 222 with one or more other devices, through a wired (e.g., Local Area Network, i.e., LAN) connection or through a wireless connection (e.g., Bluetooth®, Wi-Fi). The interface(s) 230 may also enable intercommunication between different logical as well as hardware components of the microscopy imaging system 222.

[0089] The memory(s) 232 may be a computer-readable medium, examples of which include volatile memory (e.g., RAM), and / or non-volatile memory (e.g., Erasable Programmable read-only memory, i.e., EPROM, flash memory, etc.). The memory(s) 232 may be an external memory, or internal memory, such as a flash drive, a compact disk drive, an external hard disk drive, or the like. The memory(s) 232 may further include data which either may be utilized or generated during the operation of the microscopy imaging system 222.

[0090] The microscopy imaging system 222 may further include engine(s) 234. The engine(s) 234 may be implemented as a combination of hardware and programming, for example, programmable instructions to implement a variety of functionalities of the engine(s) 234. In examples described herein, such combinations of hardware and programming may be implemented in several different ways. For example, the programming for the engine(s) 234 may be executable instructions. Such instructions may be stored on a non- transitory machine-readable storage medium which may be coupled either directly with the microscopy imaging system 222 or indirectly (for example, through networked means). In an example, the engine(s) 234 may include a processing resource, for example, either a single processor or a combination of multiple processors, to execute such instructions. In other examples, the engine(s) 234 may be implemented as electronic circuitry.

[0091] The microscopy imaging system 222 may further include data 236. The data 236, on the other hand, includes data that is either stored or generated as a result of functions implemented by any of the engine(s) 234or the microscopy imaging system 222. It may be further noted that information stored and available in the data 236 may be utilized by the engine(s) 234 for performing various functions by the microscopy imaging system 222. It may be noted that such examples are only indicative. The present approaches may be applicable to other examples without deviating from the scope of the present subject matter.

[0092] Further, in the network environment 220, the microscopy imaging system 222 may include the portable imaging system 100 having the compact optical microscopy device 102 as depicted in FIG.1A. The processing unit 228 of the microscopy imaging system 222 is communicatively coupled to the compact optical microscopy device 102. A high-quality image captured by the compact optical microscopy device 102 is further processed by the microscopy imaging system 222.

[0093] In operation, the image sensor 106 may capture an image of an object through the optical lens assembly 104. Due to the configurations of the image sensor 106 and the optical lens assembly 104, the captured image is of extremely high quality. In an example, the captured image may be transmitted to one of an inter device, an intra device, and a cloud device and the received image on one of the inter device, the intra device, and the cloud device may be visually inspected by an expert, such as a qualified medical officer to diagnose the sample for which the image has been captured. Further, the qualified medical officer may suggest remedies in light of the diagnosis. In an example, the received image may be compared with a database having prestored images to diagnose the sample for which the image has been captured. Further, the remedies in light of the diagnosis may be suggested.

[0094] Further, the processing unit 228 may process the captured image by converting the image to a digitized form. The digitized form of the image is readily interpreted by any processing unit for any kind of analysis. In case the processing unit 228 is disposed within the compact optical microscopy device 102, the image gets processed within a single device, such as amobile phone. In case the processing unit 228 is disposed outside the compact optical microscopy device 102, the image gets processed externally and the processed image may be sent back either to the compact optical microscopy device 102 or to any other external device.

[0095] The processing unit 228 may detect a unique or multiple characteristic feature of the object by analyzing the digitized form of the image using an artificial intelligence and machine learning based model. The artificial intelligence and machine learning based model may use the digitized form of the image provided by the processing unit 228 for recognizing parameters in the digitized form of the image. The recognized parameters are indicative of the unique or multiple characteristic feature of the object. In an example, processing the captured image may include resizing the image to a specific resolution, normalizing pixel values of the image, and augmenting the image data. Common data augmentation techniques include random rotation, flipping, and brightness adjustments. By processing the captured images, the images in a training set are consistently formatted, normalized to a common range, and augmented to increase the diversity of the data. The pre-processed images thus help the artificial intelligence and machine learning based model to focus on unique or multiple characteristic features during training and improve robustness of the model to variations in the input data. In an example, the artificial intelligence and machine learning based model may be trained based on the detected characteristic features of the object. Based on such trained artificial intelligence and machine learning based models, helpful applications for image recognition may be created.

[0096] In an example, the processing unit 228 may process a data set through a neural network used as the artificial intelligence and machine learning based model. The data set may pertain to an input data with a data matrix having N features. N features indicate the number of neurons in the data matrix, where each neuron represents a measurable piece of data thatcan be used for learning of the neural network. In an example, the data set may be the digitized form of the image of the object.

[0097] In an example, the neural network is an auto-encoder having an encoder-decoder based deep learning architecture. The encoder-decoder based deep learning architecture is trained successfully using data matrices, for example, in case of printers, completed print jobs may be used for training. For example, the encoder-decoder based deep learning architecture may include long short - term memory (LSTM) units. The LSTM units remember past data by generating a comprehensive summary of sequence in the form of last hidden state representation in reduced dimensional space. The LSTM units may summarize a sequence keeping salient features of all previous layers in the sequence. Based on the summary, the decoder may reconstruct the sequence from the last hidden state representation.

[0098] The auto-encoder may reconstruct an output from the input data through a series of non-linear transformations. During the series of nonlinear transformations, the neural network, such as auto-encoder, learns higher order features from the data set.

[0099] The processing unit 228 may further compare the detected characteristic feature with pre-stored dataset. In an example implementation, the artificial intelligence and machine learning based model may detect the unique or multiple characteristic feature of the object using a common technique called Local Binary Patterns (LBP). LBP is a texture descriptor that represents the patterns formed by comparing an intensity of a center pixel with an intensity of surrounding neighbor pixel. The LBP assigns a binary code to each pixel in an image based on whether an intensity of a neighboring pixel is greater or less than an intensity of the center pixel.

[0100] For example, given an image patch with pixel intensities represented as l(x, y), where (x, y) are the pixel coordinates, and N(i, j) is a set ofneighboring pixels, the LBP value at the center pixel (x, y) is computed as follows:LBP(x, y) = Z [s(l(i, j) - l(x, y)) « p] for (i, j) e N(x, y)

[0101] Here, s() is the sign function, and « denotes bitwise left shift, p is a threshold, and the LBP value is a binary number represented in decimal notation.

[0102] In another example implementation, the artificial intelligence and machine learning based model may detect the unique or multiple characteristic feature of the object using deep learning feature extraction performed by convolutional neural networks (CNNs). During the training process, the CNNs learn to automatically extract characteristics features from raw images. The CNNs involve convolutional operations and nonlinear activation functions. The convolutional operations are performed by convolutional layers in CNNs. The convolutional layers may apply filters (kernels) to the input image to extract the unique or multiple characteristic features. The convolution operation can be mathematically represented as follows:C(x, y) = Z [l(i, j) * K(x-i, y-j)] for (i, j) e N(x, y)

[0103] Here, l(i, j) is the pixel intensity at location (i, j) in the input image, K(x-i, y-j) is the value of the convolutional filter at relative coordinates (x-i, y-j), and N(x, y) is the set of neighboring pixels around position (x, y).

[0104] After the convolution operation, a non-linear activation function (e.g., ReLU - Rectified Linear Unit) is applied element-wise to the convolutional feature maps to introduce non-linearity into the model.ReLU(x) = max(0, x)

[0105] This allows the network to learn complex and nonlinear relationships between the unique or multiple characteristic features. Deep learning-based feature extraction eliminates the need for hand-crafting features, making theimage processing more flexible and capable of learning intricate patterns directly from the data.

[0106] The pre-stored dataset is a dataset stored in the database 224 that include different parameters of an object. For example, if the object is a blood sample, different parameters stored as pre-stored dataset may be a number of red blood cells count for being qualified as a healthy blood sample. For the comparison, the processing unit 228 may map the detected characteristic feature with a relevant parameter, of the object, present in the pre-stored dataset. In an example implementation, Convolutional Neural Networks (CNNs) may be used for mapping detected characteristic feature with a relevant parameter due to their ability to learn hierarchical features from images. Depending on the complexity of the task and the size of the dataset, different CNN architectures such as VGG, ResNet, Inception, or more recent ones like EfficientNet or DenseNet may be selected. On determining that the detected characteristic feature is mapped with the relevant parameter of the object, the processing unit 228 may generate a report based on the comparison. In an example implementation, the artificial intelligence and machine learning based model may quantify a category of the detected characteristic feature of the object using a state-of-the-art CNN architecture, i.e., EfficientNet that achieves impressive performance across various image classification tasks while being computationally efficient. The report is to identify a category of the detected characteristic feature of the object with or without difference or similarity in the detected characteristic feature. For example, if the mapping indicates low count of the red blood cells, then the identified category may be anemia. The category will have different segments depending on the field of application.

[0107] Further, during the operation, the processing unit 228 may transfer data related to the category of unique or multiple characteristic feature of the image to a cloud database. In an example, the cloud database may be further referred when the processing unit 228 performs the mapping.

[0108] In an example, the processing unit 228 may transfer data related to the category of unique or multiple characteristic feature of the image to one of an inter device, intra device, and cloud device using a wired or a wireless communication channel.

[0109] In an example, the microscopy imaging system 222 may include a display unit (not shown), connected to the processing unit, to display the identified category of unique or multiple characteristic feature in the form of one of an image, text, and a report. For example, the display unit may be a screen of the microscopy imaging system 222. The identified category of the unique or multiple characteristic feature displayed by the display unit is shared by the processing unit 228 to one of an inter device, an intra device, and a cloud device using a wired or a wireless communication channel.

[0110] In an example, the microscopy imaging system may include a transmitter (not shown) coupled to the processing unit 228. The processing unit may transmit the generated report via the transmitter to another device.

[0111] Some examples of the compact optical microscopy device 102 are illustrated and described with reference to FIG. 2, FIG. 3A, FIG. 3B, FIG. 3C, FIG. 3D, and FIG. 3E. In FIG. 2, FIG. 3A, FIG. 3B, FIG. 3C, FIG. 3D, and FIG. 3E, the portable imaging system 100 is further referenced with reference numeral 1 hereinafter, and the compact optical microscopy device 102 is further referenced with reference numeral 2 hereinafter. Further, the image sensor 106 is further referenced with reference numeral 4 hereinafter.

[0112] FIG. 3A illustrates a compact optical microscopy device 2, in accordance with an example of the present subject matter. The optical microscopy device 2 includes an optical lens assembly having a plurality of lens elements 2-1 , 2-2, ... , 2-N, where N may be equal to one of nine, eleven, twelve, thirteen, fourteen, and fifteen. The first lens element 2-1 may be positioned closest to the object side and farthest from the image side among the plurality of lens elements 2-1 , 2-2, ... , 2-N. The last lens element 2-N may be positioned farthest to the object side and closest to the imageside among the plurality of lens elements 2-1 , 2-2, 2-N. Various examples of the optical lens assembly of the optical microscopy device 2 are described in detail later in the description and illustrated through FIGS. 4a to 16c.

[0113] The optical lens assembly of the present subject matter enables the optical microscopy device 2 to have an optical magnification in a range of about 1 X to about 4.5X, an airy radius in a range of about 0.783 micron to about 8.89 micron, a depth of field in a range of about 2.847 micron to about 336.32 micron, a numerical aperture in a range of about 0.042 to about 0.438, a half field of view in a range of about 13 degrees to about 70 degrees, and a length in a range of about 5.51 1 millimeter (mm) to about 23.022 mm.

[0114] In an example, the first lens element 2-1 of the optical lens assembly from the object side may be configured as a wavelength-specific optical filter.

[0115] In an example, the last lens element 2-N of the optical lens assembly from the object side may be configured as a wavelength-specific optical filter.

[0116] In an example, the optical microscopy device 2 may include a wavelength-specific optical filter which may be positioned between the first lens element 2-1 and a second lens element 2-2 of the optical lens assembly from the object side.

[0117] In an example, the optical microscopy device 2 may have a wavelength-specific optical filter which may be positioned in front of the first lens element 2-1 , towards the object side.

[0118] In an example, the optical microscopy device 2 may have a wavelength-specific optical filter which may be positioned between the image sensor 4 and a last lens element 2-N of the optical lens assembly from the object side.

[0119] In an example, the optical microscopy device 2 may have a wavelength-specific optical filter which may be positioned between anadjacent pair of lens elements of the optical lens assembly. For example, a wavelength-specific optical filter may be positioned between the second lens element 2-2 and the third lens element 2-3 or between any other adjacent lens elements.

[0120] The wavelength-specific optical filter may allow a specific wavelength of light to pass therethrough and enable filter field microscopy.

[0121] In an example, the optical microscopy device 2 may include a polarized filter. The polarized filter may be disposed in front of a first lens element 2-1 of the optical lens assembly, towards the object side. The polarized filter may be included to enable polarized microscopy.

[0122] In an example, the optical microscopy device 2 may include a protective glass cover. The protective glass cover may be disposed in front of a first lens element 2-1 of the optical lens assembly, towards the object side. The protective glass cover may be included in the optical microscopy device 2 to protect the optical microscopy device 2 or the optical lens assembly from physical and chemical stress or unwanted elements such as water, dust, etc. In an example, the protective glass may be scratch resistant and chemical resistant.

[0123] The optical microscopy device 2 also includes an image sensor 4. Examples of the image sensor 4 may include, but are not limited to, a CMOS sensor and a CDD sensor. The image sensor 4 may be a camera or a part of the camera. The image sensor 4 may be positioned adjacent to the optical lens assembly of the optical microscopy device 2 to image an object in field of view of the optical lens assembly. The image sensor 4 may include wavelength specific filter or IR filter for light field and filter field microscopy. In an example, the image sensor 4 has a pixel pitch in a range of about 0.2 micron to about 6 micron. The image sensor 4 is of 0.3 megapixel (MP) to 350 MP.

[0124] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.2 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0125] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.14 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0126] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0127] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0128] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.1 1 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0129] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.10 micron, and the optical lens assembly has an optical magnification of about 2X.

[0130] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.08 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0131] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.0769 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0132] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.07 micron, and the optical lens assembly has an optical magnification of about 3X.

[0133] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.05 micron, and the optical lens assembly has an optical magnification of about 4X.

[0134] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.044micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0135] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.04 micron, and the optical lens assembly has an optical magnification of about 5X.

[0136] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.03 micron, and the optical lens assembly has an optical magnification of about 6X.

[0137] In an example, the image sensor 4 has a pixel pitch of about 0.2 micron, the optical microscopy device has a resolution of about 0.03 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0138] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.4 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0139] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.29 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0140] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.27 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0141] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.25 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0142] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.22 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0143] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.2 micron, and the optical lens assembly has an optical magnification of about 2X.

[0144] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.17 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0145] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.153 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0146] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 3X.

[0147] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.10 micron, and the optical lens assembly has an optical magnification of about 4X.

[0148] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.088 micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0149] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.08 micron, and the optical lens assembly has an optical magnification of about 5X.

[0150] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.07 micron, and the optical lens assembly has an optical magnification of about 6X.

[0151] In an example, the image sensor 4 has a pixel pitch of about 0.4 micron, the optical microscopy device has a resolution of about 0.05 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0152] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 1 X

[0153] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.36 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0154] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.33 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0155] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.31 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0156] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.28 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0157] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.25 micron, and the optical lens assembly has an optical magnification of about 2X.

[0158] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.21 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0159] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.17 micron, and the optical lens assembly has an optical magnification of about 3X.

[0160] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 4X.

[0161] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.10 micron, and the optical lens assembly has an optical magnification of about 5X.

[0162] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.08 micron, and the optical lens assembly has an optical magnification of about 6X.

[0163] In an example, the image sensor 4 has a pixel pitch of about 0.5 micron, the optical microscopy device has a resolution of about 0.06 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0164] In an example, the image sensor 4 has a pixel pitch of about 0.56 micron, the optical microscopy device has a resolution of about 0.56 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0165] In an example, the image sensor 4 has a pixel pitch of about 0.56 micron, the optical microscopy device has a resolution of about 0.37 micron, and the optical lens assembly has an optical magnification of about 1 ,5X.ln an example, the image sensor 4 has a pixel pitch of about 0.56 micron, the optical microscopy device has a resolution of about 0.28 micron, and the optical lens assembly has an optical magnification of about 2X.ln an example, the image sensor 4 has a pixel pitch of about 0.56 micron, the optical microscopy device has a resolution of about 0.215 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0166] In an example, the image sensor 4 has a pixel pitch of about 0.56 micron, the optical microscopy device has a resolution of about 0.124 micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0167] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.60 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0168] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.43 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0169] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.40 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0170] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.38 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0171] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.33 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0172] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.30 micron, and the optical lens assembly has an optical magnification of about 2X.

[0173] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.25 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0174] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.20 micron, and the optical lens assembly has an optical magnification of about 3X.

[0175] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.15 micron, and the optical lens assembly has an optical magnification of about 4X.

[0176] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.12 micron, and the optical lens assembly has an optical magnification of about 5X.

[0177] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.10 micron, and the optical lens assembly has an optical magnification of about 6X.

[0178] In an example, the image sensor 4 has a pixel pitch of about 0.6 micron, the optical microscopy device has a resolution of about 0.08 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0179] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.70 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0180] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0181] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.47 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0182] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.44 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0183] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.39 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0184] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.35 micron, and the optical lens assembly has an optical magnification of about 2X.

[0185] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.29 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0186] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.23 micron, and the optical lens assembly has an optical magnification of about 3X.

[0187] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.18 micron, and the optical lens assembly has an optical magnification of about 4X.

[0188] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.14 micron, and the optical lens assembly has an optical magnification of about 5X.

[0189] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.12 micron, and the optical lens assembly has an optical magnification of about 6X.

[0190] In an example, the image sensor 4 has a pixel pitch of about 0.7 micron, the optical microscopy device has a resolution of about 0.09 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0191] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.80 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0192] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.57 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0193] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.53 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0194] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0195] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.44 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0196] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.40 micron, and the optical lens assembly has an optical magnification of about 2X.

[0197] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.33 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0198] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.27 micron, and the optical lens assembly has an optical magnification of about 3X.

[0199] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.20 micron, and the optical lens assembly has an optical magnification of about 4X.

[0200] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.16 micron, and the optical lens assembly has an optical magnification of about 5X.

[0201] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 6X.

[0202] In an example, the image sensor 4 has a pixel pitch of about 0.8 micron, the optical microscopy device has a resolution of about 0.10 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0203] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.90 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0204] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.64 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0205] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.60 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0206] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.56 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0207] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0208] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.45 micron, and the optical lens assembly has an optical magnification of about 2X.

[0209] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.38 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0210] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.30 micron, and the optical lens assembly has an optical magnification of about 3X.

[0211] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.23 micron, and the optical lens assembly has an optical magnification of about 4X.

[0212] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.18 micron, and the optical lens assembly has an optical magnification of about 5X.

[0213] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.15 micron, and the optical lens assembly has an optical magnification of about 6X.

[0214] In an example, the image sensor 4 has a pixel pitch of about 0.9 micron, the optical microscopy device has a resolution of about 0.12 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0215] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 1 .00 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0216] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.71 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0217] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.67 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0218] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.63 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0219] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.56 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0220] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 2X.

[0221] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.42 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0222] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.33 micron, and the optical lens assembly has an optical magnification of about 3X.

[0223] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.25 micron, and the optical lens assembly has an optical magnification of about 4X.

[0224] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.20 micron, and the optical lens assembly has an optical magnification of about 5X.

[0225] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.17 micron, and the optical lens assembly has an optical magnification of about 6X.

[0226] In an example, the image sensor 4 has a pixel pitch of about 1 micron, the optical microscopy device has a resolution of about 0.13 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0227] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 1.12 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0228] In an example, the image sensor 4 has a pixel pitch of about 1 .12 micron, the optical microscopy device has a resolution of about 0.80 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0229] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.75 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0230] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.70 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0231] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.62 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0232] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.56 micron, and the optical lens assembly has an optical magnification of about 2X.

[0233] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.47 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0234] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.43 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0235] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.37 micron, and the optical lens assembly has an optical magnification of about 3X.

[0236] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.28 micron, and the optical lens assembly has an optical magnification of about 4X.

[0237] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.248 micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0238] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.22 micron, and the optical lens assembly has an optical magnification of about 5X.

[0239] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.19 micron, and the optical lens assembly has an optical magnification of about 6X.

[0240] In an example, the image sensor 4 has a pixel pitch of about 1.12 micron, the optical microscopy device has a resolution of about 0.14 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0241] In an example, the image sensor 4 has a pixel pitch of about 1.4 micron, the optical microscopy device has a resolution of about 1 .40 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0242] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 1 .00 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0243] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.93 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0244] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.88 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0245] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.78 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0246] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.70 micron, and the optical lens assembly has an optical magnification of about 2X.

[0247] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.58 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0248] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.47 micron, and the optical lens assembly has an optical magnification of about 3X.

[0249] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.35 micron, and the optical lens assembly has an optical magnification of about 4X.

[0250] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.28 micron, and the optical lens assembly has an optical magnification of about 5X.

[0251] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.23 micron, and the optical lens assembly has an optical magnification of about 6X.

[0252] In an example, the image sensor 4 has a pixel pitch of about 1 .4 micron, the optical microscopy device has a resolution of about 0.18 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0253] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 3 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0254] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 2.14 micron, and the optical lens assembly has an optical magnification of about 1 .4X.

[0255] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 2 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0256] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1 .88 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0257] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1 .67 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0258] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1 .5 micron, and the optical lens assembly has an optical magnification of about 2X.

[0259] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1 .25 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0260] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1.153 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0261] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 1 .00 micron, and the optical lens assembly has an optical magnification of about 3X.

[0262] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 0.75 micron, and the optical lens assembly has an optical magnification of about 4X.

[0263] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 0.66 micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0264] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 0.60 micron, and the optical lens assembly has an optical magnification of about 5X.

[0265] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 0.50 micron, and the optical lens assembly has an optical magnification of about 6X.

[0266] In an example, the image sensor 4 has a pixel pitch of about 3 micron, the optical microscopy device has a resolution of about 0.38 micron, and the optical lens assembly has an optical magnification of about 7.8X.

[0267] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 6 micron, and the optical lens assembly has an optical magnification of about 1 X.

[0268] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 4.29 micron, and the optical lens assembly has an optical magnification of about 1 ,4X.ln an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 4 micron, and the optical lens assembly has an optical magnification of about 1 .5X.

[0269] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 3.75 micron, and the optical lens assembly has an optical magnification of about 1 .6X.

[0270] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 3.33 micron, and the optical lens assembly has an optical magnification of about 1 .8X.

[0271] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 3 micron, and the optical lens assembly has an optical magnification of about 2X.

[0272] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 2.50 micron, and the optical lens assembly has an optical magnification of about 2.4X.

[0273] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 2.307 micron, and the optical lens assembly has an optical magnification of about 2.6X.

[0274] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 2.00 micron, and the optical lens assembly has an optical magnification of about 3X.

[0275] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 1 .50 micron, and the optical lens assembly has an optical magnification of about 4X.

[0276] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 1 .33 micron, and the optical lens assembly has an optical magnification of about 4.5X.

[0277] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 1 .20 micron, and the optical lens assembly has an optical magnification of about 5X.

[0278] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 1 micron, and the optical lens assembly has an optical magnification of about 6X.

[0279] In an example, the image sensor 4 has a pixel pitch of about 6 micron, the optical microscopy device has a resolution of about 0.77 micron, and the optical lens assembly has an optical magnification of about 7.8X.Table 1 tabulates different resolution with respect to sensor pixel pitch and optical magnification.

[0280] The optical microscopy device 2 when embedded inside a portable imaging system 1 enables microscopy feature in the portable imaging system 1 . The image sensor 4 of the optical microscopy device 2 enables high-resolution microscopic features in the portable imaging system 1 . The optical microscopy device 2 may have a resolution of about 0.03 micron to about 6 micron. In other words, the effective resolution after combining with the pixel pitch of the image sensor 4 is about 0.03 micron to about 6 micron. Thus, the optical microscopic device 2 may be used to see micron sized objects, such as objects having size as small as 0.03 micron.

[0281] In an example, the quantitative and qualitative analysis of the image(s) captured through the optical microscopy device 2 may be performed using a conventional algorithm or software.

[0282] FIG. 3B illustrates an optical microscopy device 2, in accordance with another example of the present subject matter. The optical microscopy device 2 of FIG. 3B includes the optical lens assembly, similar to as described for FIG. 2. Further, the optical microscopy device 2 of FIG. 3Bincludes an image sensor 5 which may be similar to the image sensor 4 as described for FIG. 2. Additionally, the optical microscopy device 2 of FIG. 3B includes a focusing unit 6 having a barrel. The optical lens assembly is disposed within the barrel of the focusing unit 6. The focusing unit 6 is configured to operate the optical lens assembly for focusing while imaging an object.

[0283] In an example, the focusing unit 6 has a controlled movement for focusing an object. The mechanism involved in the movement of the focusing unit 6 may include, but is not limited to, a piezoelectric movement mechanism or an electromagnetic movement mechanism. It may be noted that a software or a mobile application may be utilized for controlling the movement of the focusing unit 6 for focusing of an object.

[0284] In an example, a light guiding unit may be placed at a location of a protective element or it can be located between the image sensor and the last lens element or between any lens element of the microscopic lens assembly. The light guiding element may be wavelength specific filter such as dichroic filter, gelatine filter or glass filter or coated polymeric film or composite which can allow to pass the specific wavelength of light in range from 200nm to 700 nm.

[0285] In an example, the portable imaging system 1 in which the optical microscopy device 2 is embedded may provide an auto-focusing feature. The auto-focusing feature provides flexibility for observing objects of different sizes and located at the various distance with high resolution, wide field of view, and without any spherical and optical aberrations. The autofocusing and high-resolution image capturing ability of the optical microscopy device 2 provides more user-friendly portable imaging system for viewing objects without any external attachment which is otherwise conventionally used.

[0286] FIG. 3C illustrates the optical microscopy device 2, in accordance with another example of the present subject matter. The optical microscopy device 2 of FIG. 3C includes the optical lens assembly and the focusing unit6, similar to as described for FIG. 3B. In one example, the optical microscopy device 2 of FIG. 3D may include a light source 3 placed adjacent to the optical lens assembly to emit light such that the emitted light is incident on a sample held within a sample holder 12, when the sample holder 12 is placed over the optical lens assembly. The sample holder 12 may be a transparent chamber that can hold a sample. The sample holder 12 may hold a glass slide 14 on which the sample may be placed. Such an arrangement when the light source 3 is placed adjacent to the optical lens assembly of the optical microscopy device 2 to emit the light such that the emitted light is incident on the sample held within the sample holder 12 is shown in FIG. 17. In an example, the sample holder 12 may be detachably coupled to the optical microscopy device 2. In an example, the e light source 3 may be placed adjacent to the sample holder 12. In an example, the light source 3 is an external light source.

[0287] The sample holder 12 may be comprised of object slides with single or multiple compartments or microfluidic channels for the single or multiple sample analysis for continuous or batch observation. The sample holder 12 may have X-Y direction movement which is controlled manually or automated through the rotating wheel and gear mechanism for fine movement in X- Y direction. The sample holder 12 may include the wavelength-specific coating or filter or polarized filter for passing specific wavelengths of light coming from the object on exposure to the external excitation light. The sample holder 12 may be attached to the smartphone or any digital device through the single arm or two arm clip-on arrangement or magnetic attachment or adjustable screw-based attachment or direct smartphone cover-based arrangement for an attachment to the smartphone etc.

[0288] In an example, the light source 3 may include at least one LED placed at a circumferential boundary of the optical lens assembly towards the object side. The at least one LED may be placed such that the light is emitted in a form of a ring. In an example, the LED may be a white LED,optionally, with diffuser. The white LED may be used to enable light field microscopy. In another example, the LED may be a wavelength-specific LED to emit light of a wavelength in a range of about 200 nm to about 700 nm on the sample held within a sample holder 12. The wavelength-specific LED may be used to enable filter field microscopy.

[0289] In an example, the LED may be placed such that the emitted light is incident on the sample held within the sample holder 12 at an angle in a range of about 5° to about 90° with respect to a planar surface 13 of the sample holder 12.

[0290] In an example, the LED may be placed such that the emitted light is incident on the sample held within the sample holder 12 at an angle in a range of about 48° to about 49° with respect to a planar surface 13 of the sample holder 12.

[0291] In an example, the LED may be placed such that the emitted light is incident on the sample held within the sample holder 12 at an angle of about 48.5° with respect to a planar surface 13 of the sample holder 12.

[0292] The angle of the emitted light with respect to the planar surface 13 is important for total internal reflection of light inside the sample holder 12, specifically for filter field microscopy.

[0293] FIG. 3D illustrates an optical microscopy device 2, in accordance with another example of the present subject matter. The optical microscopy device 2 of FIG. 3D includes the optical lens assembly, the focusing unit 6, and the light source 3, similar to as described for FIG. 3C. Additionally, the optical microscopy device 2 of FIG. 3D includes a light focusing element 10 to direct the light from the light source 3 to the object in the sample holder 12. The light focusing element 10 may be an optical fiber or an optically transparent rod coated with the reflective materials.

[0294] FIG. 3E shows another example of the light focusing element 10 described with reference to FIG. 3D. The light focusing element 10 facilitates in directing the light at an angle in such a way that light is incident on the sample holder 12 at critical angle for the total internal reflection oflight inside the sample holder 12 or inside the glass slide 14 holding the sample.

[0295] FIG. 3F shows an example of a light focusing element 15. The light focusing element 15 is an optical film or sheet which can direct the light coming at different angles from the light source 3 to the object located above the optical microscopy device 2.

[0296] Further, the planar surface 13 of the sample holder 12 may be blackened, as shown in FIG. 3C, FIG. 3D, FIG. 3E, and FIG. 3F, and the light is made incident through the other planar surface of the sample holder 12. The sample is placed between the blackened planar surface 13 and the planar surface through which the light is made incident to view the light emit from the object against the dark background.

[0297] In an example, the light source 3 provides for a dual mode observation of object under light field and filter field, where the object in the sample illuminates light of a specific wavelength.

[0298] FIG. 4A illustrates an external light source arrangement with diffusor or polarizer, in accordance with an example of the present subject matter. The external light source arrangement includes a Light-Emitting Diode (LED) 422. The LED 422 may be located at periphery of the light source 3. In an example, the LED 422 may act as a light source. The external light source arrangement may further include a diffuser or a polarized filter 424 located below the light source 3.

[0299] FIG. 4B illustrates another example of the external light source arrangement with diffusor or polarizer. The external light source arrangement includes a Light-Emitting Diode (LED) 422. The LED 422 may be located at top of the light source 3. In an example, the LED 422 may act as a light source. The external light source arrangement may further include a diffuser or a polarized filter 424 located below the light source 3.

[0300] FIG. 4C illustrates another example of the external light source arrangement with diffusor or polarizer with reference to FIG. 4A. The LED 422 may be located at the periphery of the light source 3 and the diffuser424 is located below the light source 3. In an example, the LED 422 may act as a light source. The external light source arrangement may further include optical elements 426. The optical elements 426 may include convex or plano-convex lenses. The optical elements 426 may be located below the diffuser 424. In an example implementation, the optical elements 426 may be used to concentrate the light coming from the light source 3 towards the object slide.

[0301] FIG. 5A illustrates an external light source arrangement without diffusor, in accordance with an example of the present subject matter. The external light source arrangement includes a Light-Emitting Diode (LED) 522 and an optical lens element 524. The LED 522 may be located at the periphery of the light source 3. In an example, the LED 522 may act as a light source. The optical lens element 524 may include a convex or planoconvex lens. The optical lens element 524 may be located below the light source 3. In an example implementation, the top surface of the light source 3 may be reflective in nature.

[0302] FIG. 5B illustrates another external light source arrangement without diffusor. The external light source arrangement includes a Light-Emitting Diode (LED) 522 and an optical lens element 524. The LED 522 may be located at the top of the light source (3). In an example, the LED 522 may act as a light source. The optical lens element 524 may include a convex or plano-convex lens. The optical lens element 524 may be located below the light source 3. In an example implementation, the top surface of the light source 3 may be reflective in nature to reflect the light coming from the light source 3 towards the optical elements.

[0303] FIG. 6 illustrates an external light source arrangement for dark field and filter field microscopy, in accordance with an example of the present subject matter. The external light source arrangement includes a Light- Emitting Diode (LED) 622. The LED 622 may be located at the top of the light source 3. The external light source arrangement may further include a peripheral opening in a black filter 624 is located below the light source 3.The external light source arrangement may further include an optical lens element 626. The optical lens element 626 may include a convex or planoconvex lens located below the black filter 624. The black filter 624 only allows the peripheral light rays and the peripheral light rays passes through the optical element such as the plano-convex or convex lens and further rays concentrate on the object located below the optical lens assembly. In an example implementation, the single or multiwavelength of light rays in dark background are used for dark field or filter field microscopic characteristics.

[0304] The present subject matter was experimented in detail using in the following examples of the optical lens assembly of the optical microscopy device 2. FIG. 7A to FIG. 15C represents example layouts of the optical lens assembly of the optical microscopy devices, and associated distortion plots and modulation transfer function (MTF) plots, results of the individual embodiments of in accordance with the present subject matter. The examples describe and demonstrate embodiments within the scope of the present subject matter. The examples are provided herein solely for the purpose of illustration and is not to be construed as limitations of the present subject matter, as many variations thereof are possible without departing from spirit and scope.

[0305] The numeral values provided the present disclosure may include tolerance of about ±5%. The numeral values provided herein may have been rounded-off to the nearest decimal place for the sake of simplicity.

[0306] It may be noted that the serial numbering of the rows of the tables for each embodiment has one to one correspondence.EXAMPLES OF OPTICAL LENS ASSEMBLYExample 1

[0307] FIG. 7A illustrates an example layout diagram for a first embodiment of the optical lens assembly 2. The optical lens assembly 2 of the first embodiment comprises eleven lens elements 201 -21 1 which are sequentially arranged from object side to image side. The aperture stop 212 is located between the fifth lens element 205 and the sixth lens element 206. The first five lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0308] The first lens element 201 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 202 has a negative refracting power. The image-side surface of the second lens element 202 comprises a convex portion in a vicinity of the optical axis and a concave portion in the vicinity of the periphery. The object-side surface of the second lens element 202 comprises a concave portion in the vicinity of the optical axis. The third lens element 203 has a positive refracting power. Both the object-side surface and image-side surface of the third lens element 203 are convex. The fourth lens element 204 has a negative refracting power. The image-side surface of the fourth lens element 204 is concave near the optical axis and the object-side surface of the fourth lens element 204 is convex. The fifth lens element 205 has a positive refracting power. Both the object-side surface and the image-side surface of the fifth lens element 205 are convex. The sixth lens element 206 has a refractive power. The object-side surface of the sixth lens element 206 is convex, and the image-side surface of the sixth lens element 206 is concave. The seventh lens element 207 has a refractive power. The object-side surface of the seventh lens element 207 is concave, and the image-side surface of the seventh lens element 207 is convex. The eighth lens element 208 has a refractive power. The object-side surface of the eighth lens element 208 is concave, and the image-side surface of the eighth lens element 208 is convex. The ninth lens element 209 has a positive refractive power and boththe image-side surface and the object-side surface of the ninth lens element209 are convex in the vicinity of the optical axis. The tenth lens element 210 has a refractive power. The object-side surface of the tenth lens element210 comprises a convex portion in the vicinity of the optical axis and a concave portion in the vicinity of the periphery of the tenth lens element 210. The image-side surface of the tenth lens element 210 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the tenth lens element 210. The eleventh lens element 211 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The eleventh lens element 211 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength-specific filter may be located between the first lens element 201 and second lens element 202, or the first lens element 201 or the eleventh lens element 21 1 can act as a wavelength-specific filter in the optical lens assembly.

[0309] FIG. 7B illustrates a distortion plot for the first embodiment of the optical lens assembly. Further, FIG. 7C illustrates a MTF plot for the first embodiment of the optical lens assembly.

[0310] The optical lens assembly data for the first embodiment is shown in Table 2A and the aspheric data for the first embodiment of the optical lens assembly is shown in Table 2B. The optical lens assembly of the first embodiment has the following parameters.Magnifications = 2X; Numerical aperture = 0.104; Focal Length = 0.9718 mm; System length = 5.511 mm; HFOV = 20 degree highest HFOV; Depth of focus = 12.0155 microns; Airy radius = 3.655 microns; Depth of field (microns) = 3.0.Table 2A: The optical lens assembly data for the first embodimentTable 2B: The aspheric data for the first embodiment of the optical lens assembly

[0311] The optical lens assembly of the first embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.105 mm, a refractive index of about 1.517, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.284 mm, an image side surface of a radius of curvature of about -0.409 mm, a thickness of about 0.154 mm, a refractive index of about 1 .535, and Abbe number of about 56; a third lens element having an object side surface of a radius of curvature of about 1 .04 mm, an image side surface of a radius of curvature of about - 2.41 mm, a thickness of about 0.293 mm, a refractive index of about 1 .544, and Abbe number of about 56.1 ; a fourth lens element having an object side surface of a radius of curvature of about 0.837 mm, an image side surface of a radius of curvature of about 0.365 mm, a thickness of about 0.145 mm, a refractive index of about 1 .635, and Abbe number of about 23.9; a fifth lens element having an object side surface of a radius of curvature of about 1.03 mm, an image side surface of a radius of curvature of about - 0.75 mm, a thickness of about 0.237 mm, a refractive index of about 1 .544, and Abbe number of about 56.1 ; a sixth lens element having an object side surface of a radius of curvature of about 3.2 mm, an image side surface of a radius of curvature of about 7.87 mm, a thickness of about 0.38 mm, a refractive index of about 1 .535, and Abbe number of about 55.712; a seventh lens element having an object side surface of a radius of curvature of about -10 mm, an image side surface of a radius of curvature of about -1 .1 mm, a thickness of about 0.471 mm, a refractive index of about 1 .535, and Abbe number of about 55.712;an eighth lens element having an object side surface of a radius of curvature of about -0.471 mm, an image side surface of a radius of curvature of about -0.772 mm, a thickness of about 0.22 mm, a refractive index of about 1 .638, and Abbe number of about 23.256; a ninth lens element having an object side surface of a radius of curvature of about 4.58 mm, an image side surface of a radius of curvature of about - 0.878 mm, a thickness of about 0.694 mm, a refractive index of about 1 .535, and Abbe number of about 55.712; a tenth lens element having an object side surface of a radius of curvature of about 2.83 mm, an image side surface of a radius of curvature of about 0.775 mm, a thickness of about 0.482 mm, a refractive index of about 1 .638, and Abbe number of about 23.256; and an eleventh lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .517, and Abbe number of about 66.2, where the optical lens assembly comprises an aperture stop positioned between the fifth lens element and the sixth lens element, where the aperture stop is a planar element having a thickness of about 0.05 mm.

[0312] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, a wavelength specific filter, or combination thereof, for multimodal microscopy.Example 2

[0313] FIG. 8A illustrates an example layout diagram for a second embodiment of the optical lens assembly 2. The optical lens assembly 2 of the second embodiment comprises fourteen lens elements 501 -514 which are sequentially arranged from object side to image side. The aperture stop 515 is located between the eighth lens element 508 and the ninth lens element 509. The first eight lens elements can act as an objective assemblywhich can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0314] The first lens element 501 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 502 has a refracting power. The object-side surface of the second lens element 502 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the second lens element 502. The image-side surface of the second lens element 502 comprises a concave portion in the vicinity of the optical axis. The third lens element 503 has a positive refracting power. The image-side surface of the third lens element 503 comprises a convex portion in a vicinity of the optical axis and a concave portion in the vicinity of the periphery of the third lens element 503. The object-side surface of the third lens element 503 comprises a concave portion in a vicinity of the optical axis and a convex portion in the peripheral vicinity. The fourth lens element 504 has a positive refracting power. The image-side surface of the fourth lens element 504 comprises a concave portion and is a meniscus in the vicinity of the optical axis. The object-side surface of the fourth lens element 504 comprises a convex portion and is a meniscus in the vicinity of the optical axis. The fifth lens element 505 has a positive refracting power. The object-side surface of the fifth lens element 505 is convex. The sixth lens element 506 has a negative refracting power. The image-side surface of the sixth lens element 506 is a concave surface comprising a concave portion in the vicinity of the optical axis. The seventh lens element 507 has a refracting power. The object-side surface of the seventh lens element 507 is concave. The eighth lens element 508 has a positive refracting power. The image-side surface of the eighth lens element 508 is convex. The ninth lens element 509 has a refractive power. The object-side surface of the ninth lens element 509 is convex, and the imageside surface of the ninth lens element 509 is concave. The tenth lens element 510 has a refractive power. The object-side surface of the tenthlens element 510 is concave, and the image-side surface of the tenth lens element 510 is convex. The eleventh lens element 511 has a refractive power. The object-side surface of the eleventh lens element 511 is concave, and the image-side surface of the eleventh lens element 51 1 is convex. The twelfth lens element 512 has a refractive power and both the image-side surface and the object-side surface of the twelfth lens element 512 are convex in the vicinity of the optical axis. The thirteenth lens element 513 has a refractive power. The object-side surface of the thirteenth lens element 513 comprises a convex portion in a vicinity of the optical axis and a concave portion in the vicinity of the periphery of the thirteenth lens element 513. The image-side surface of the thirteenth lens element 513 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the thirteenth lens element 513. The fourteenth lens element 514 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The fourteenth lens element 514 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 501 and the second lens element 502, or the first lens element 501 or the fourteenth lens element 514 can act as a wavelength-specific filter in the optical lens assembly.

[0315] FIG. 8B illustrates a distortion plot for the second embodiment of the optical lens assembly. Further, FIG. 8C illustrates a MTF plot for the second embodiment of the optical lens assembly.

[0316] The optical lens assembly data for the second embodiment is shown in Table 3A and the aspheric data for the second embodiment of the optical lens assembly is shown in Table 3B. The optical lens assembly of the second embodiment has the following parameters.Magnifications = 1 X ; Numerical aperture = 0.142 ; Focal Length = 1.34863 mm ; System length = 7.81620 mm ; HFOV = 31.83 degree highest HFOV ; Depth of focus = 2.47 microns ; Airy radius = 31 .83 microns ; Depth of field (microns) = 31 .83.Table 3A: The optical lens assembly data for the second embodimentTable 3B: The aspheric data for the second embodiment of the optical lens assembly

[0317] The optical lens assembly of the second embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.105 mm, a refractive index of about 1.52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.331 mm, an image side surface of a radius of curvature of about 0.424 mm, a thickness of about 0.19 mm, a refractive index of about 1 .51 , and Abbe number of about 56.5;a third lens element having an object side surface of a radius of curvature of about -0.606 mm, an image side surface of a radius of curvature of about -1 .28 mm, a thickness of about 0.249 mm, a refractive index of about 1 .54, and Abbe number of about 56; a fourth lens element having an object side surface of a radius of curvature of about 1 .08 mm, an image side surface of a radius of curvature of about 1 .64 mm, a thickness of about 0.185 mm, a refractive index of about 1 .61 , and Abbe number of about 26.9; a fifth lens element having an object side surface of a radius of curvature of about 0.404 mm, an image side surface of a radius of curvature of about - 0.124 mm, a thickness of about 0.303 mm, a refractive index of about 1 .54, and Abbe number of about 56; a sixth lens element having an object side surface of a radius of curvature of about -0.233 mm, an image side surface of a radius of curvature of about -0.107 mm, a thickness of about 0.1 16 mm, a refractive index of about 1 .67, and Abbe number of about 19.5; a seventh lens element having an object side surface of a radius of curvature of about -0.91 1 mm, an image side surface of a radius of curvature of about -0.521 mm, a thickness of about 0.1 15 mm, a refractive index of about 1 .61 , and Abbe number of about 26.9; an eighth lens element having an object side surface of a radius of curvature of about -0.0225 mm, an image side surface of a radius of curvature of about -0.868 mm, a thickness of about 0.392 mm, a refractive index of about 1 .54, and Abbe number of about 56; a ninth lens element having an object side surface of a radius of curvature of about 0.313 mm, an image side surface of a radius of curvature of about 0.127 mm, a thickness of about 0.38 mm, a refractive index of about 1.53, and Abbe number of about 55.7;a tenth lens element having an object side surface of a radius of curvature of about -0.0999 mm, an image side surface of a radius of curvature of about -0.913 mm, a thickness of about 0.471 mm, a refractive index of about 1 .53, and Abbe number of about 55.7; an eleventh lens element having an object side surface of a radius of curvature of about -2.12 mm, an image side surface of a radius of curvature of about -1 .30 mm, a thickness of about 0.22 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a twelfth lens element having an object side surface of a radius of curvature of about 0.218 mm, an image side surface of a radius of curvature of about -1.14 mm, a thickness of about 0.694 mm, a refractive index of about 1 .53, and Abbe number of about 55.7; a thirteenth lens element having an object side surface of a radius of curvature of about 0.354 mm, an image side surface of a radius of curvature of about 1 .29 mm, a thickness of about 0.482 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; and a fourteenth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .515, and Abbe number of about 64.1 , where the optical lens assembly comprises an aperture stop positioned between the eighth lens element and the ninth lens element, where the aperture stop is a planar element having a thickness of about 0.5 mm.

[0318] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, a wavelength specific filter, or combination thereof, for multimodal microscopy.Example 3

[0319] FIG. 9A illustrates an example layout diagram for a third embodiment of the optical lens assembly 2. The optical lens assembly 2 of the third embodiment comprises twelve lens elements 601 -612 which are sequentially arranged from object side to image side. The aperture stop 613 is located between the sixth lens element 606 and the seventh lens elements 607. The first six lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0320] The first lens element 601 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 602 has a positive refractive power. The image-side surface of the second lens element 602 includes a convex portion in the vicinity of the optical axis, and a concave portion in the vicinity of the periphery of the second lens element 602. The object-side surface of the second lens element 602 includes a concave portion in the vicinity of the optical axis and a convex portion in the vicinity of the periphery. The third lens element 603 has a negative refractive power. The image-side surface of the third lens element 603 is convex near the optical axis and the object-side surface of the third lens element 603 is concave near the optical axis. The fourth lens element 604 has a positive refractive power. The image-side surface of the fourth lens element 604 is concave and the object-side surface of the fourth lens element 604 is convex. The fifth lens element 605 has a negative refractive power. Both the image-side surface and the object-side surface of the fifth lens element 605 are concave. The sixth lens element 606 has a positive refractive power. The image-side surface of the sixth lens element 606 is convex and the object-side surface of the sixth lens element 606 is either plane or convex. The seventh lens element 607 has a positive refracting power. Both the object-side surface and the image-side surface of the seventh lens element 607 are convex. The eighth lens element 608 has a negative refracting power. The image-side surface of the eighth lens element 608 isconcave. The ninth lens element 609 has a refracting power. The objectside surface of the ninth lens element 609 is concave, and the image-side surface of the ninth lens element 609 is convex. The tenth lens element 610 has a positive refracting power. The object-side surface of the tenth lens element 610 is concave near the vicinity of the optical axis and the imageside surface of the tenth lens element 610 is convex near the optical axis. The eleventh lens element 61 1 has a negative refracting power. The objectside surface of the eleventh lens element 61 1 comprises a concave portion in the vicinity of the optical axis. The image-side surface of the eleventh lens element 61 1 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the eleventh lens element 61 1 . The twelfth lens element 612 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The twelfth lens element 612 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 601 and the second lens element 602 or the first lens element 601 or the twelfth lens element 612 can act as a wavelength-specific filter in the optical lens assembly.

[0321] FIG. 9B illustrates a distortion plot for the third embodiment of the optical lens assembly. Further, FIG. 9C illustrates a MTF plot for the third embodiment of the optical lens assembly.

[0322] The optical lens assembly data for the third embodiment is shown in Table 4A and the aspheric data for the third embodiment of the optical lens assembly is shown in Table 4B. The optical lens assembly of the third embodiment has the following parameters.Magnifications = 1 X; Numerical aperture = 0.042; Focal Length = 1.75326; System length = 10.6851 mm; HFOV = 23 degree highest HFOV; Depth of field = 336.32 microns; Airy radius = 8.575 microns.Table 4A: The optical lens assembly data for the third embodimentTable 4B: The aspheric data for the third embodiment of the optical lens assembly

[0323] The optical lens assembly of the third embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.849 mm, an image side surface of a radius of curvature of about -1 .05 mm, a thickness of about 0.46 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a third lens element having an object side surface of a radius of curvature of about -1 .04 mm, an image side surface of a radius of curvature of about -0.686 mm, a thickness of about 0.373 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a fourth lens element having an object side surface of a radius of curvature of about 0.763 mm, an image side surface of a radius of curvature of about 0.64 mm, a thickness of about 0.583 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a fifth lens element having an object side surface of a radius of curvature of about 0.0448 mm, an image side surface of a radius of curvature of about 0.272 mm, a thickness of about 0.3 mm, a refractive index of about 1.63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about 0.0328 mm, an image side surface of a radius of curvature of about-0.481 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a seventh lens element having an object side surface of a radius of curvature of about 0.535 mm, an image side surface of a radius of curvature of about -0.0777 mm, a thickness of about 0.99 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eighth lens element having an object side surface of a radius of curvature of about -0.0129 mm, an image side surface of a radius of curvature of about 0.319 mm, a thickness of about 0.3 mm, a refractive index of about 1.63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.128 mm, an image side surface of a radius of curvature of about -0.186 mm, a thickness of about 0.49 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a tenth lens element having an object side surface of a radius of curvature of about -0.268 mm, an image side surface of a radius of curvature of about -0.579 mm, a thickness of about 0.89 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eleventh lens element having an object side surface of a radius of curvature of about -0.267 mm, an image side surface of a radius of curvature of about 0.298 mm, a thickness of about 0.5 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 , where the optical lens assembly comprises an aperture stop positioned between the sixth lens element and the seventh lens element, where the aperture stop is a planar element having a thickness of about 0.186 mm.

[0324] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, or a wavelength specific filter, or combination thereof, for multimodal microscopy.Example 4

[0325] FIG. 10A illustrates an example layout diagram for a fourth embodiment of the optical lens assembly 2. The optical lens assembly 2 of the fourth embodiment comprises twelve lens elements 701 -712 which are sequentially arranged from object side to image side. The aperture stop 713 is located between the sixth lens element 706 and the seventh lens elements 707. The first six lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0326] The first lens element 701 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 702 has a negative refractive power. The image-side surface of the second lens element 702 includes a concave portion in the vicinity of the optical axis and the object-side surface of the second lens element 702 is concave in the vicinity of the optical axis but convex in the vicinity of the periphery. The third lens element 703 has a positive refractive power. The object-side surface of the third lens element 703 is convex and the image-side surface of the third lens element 703 is concave in the vicinity of the axis. The fourth lens element 704 has a refractive power. The object-side surface of the fourth lens element 704 includes a convex portion in the vicinity of the axis and the image-side surface of the fourth lens element 704 is concave. The fifth lens element 705 has a negative refractive power. The object-side surface of the fifth lens element 705 is concave and the image-side surface of the fifth lens element 705 is concave. The sixth lens element 706 has a positive refractive power. The image-side surface of the sixth lens element706 includes a convex portion in the vicinity of the periphery. The seventh lens element 707 has a positive refracting power. The object-side surface of the seventh lens element 707 is convex and meniscus. The eighth lens element 708 has a negative refracting power. The object-side surface of the eighth lens element 708 is a concave surface comprising a concave portion in the vicinity of the optical and is a meniscus. The image-side surface of the eighth lens element 708 is a convex surface comprising a convex portion in the vicinity of the periphery of the eighth lens element 708 and is the meniscus. The ninth lens element 709 has a positive refracting power. The object-side surface of the ninth lens element 709 is concave near the vicinity of the optical axis and is a meniscus. The image-side surface of the ninth lens element 709 is convex near the optical axis. The tenth lens element 710 has a negative refracting power. The object-side surface of the tenth lens element 710 comprises a convex portion in a vicinity of the optical axis and a concave portion in the vicinity of the periphery of the tenth lens element 710. The eleventh lens element 71 1 has a positive refracting power. The image-side surface of the eleventh lens element 711 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the eleventh lens element 71 1 . The twelfth lens element 712 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The twelfth lens element 712 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 701 and the second lens element 702 or the first lens element 701 or the twelfth lens element 712 can act as a wavelength-specific filter in the optical lens assembly.

[0327] FIG. 10B illustrates a distortion plot for the fourth embodiment of the optical lens assembly. Further, FIG. 10C illustrates a MTF plot for the fourth embodiment of the optical lens assembly.

[0328] The optical lens assembly data for the fourth embodiment is shown in Table 5A and the aspheric data for the fourth embodiment of the opticallens assembly is shown in Table 5B. The optical lens assembly of the fourth embodiment has the following parameters.Magnifications = 1 X; Numerical aperture = 0.103; Focal Length = 1.45725;System length = 8.33162 mm; HFOV = 21 degree highest HFOV; Depth of field = 55.51 microns; Airy radius = 3.487 microns.Table 5A: The optical lens assembly data for the fourth embodimentTable 5B: The aspheric data for the fourth embodiment of the optical lens assembly

[0329] The optical lens assembly of the fourth embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.075 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -0.596 mm, an image side surface of a radius of curvature of about 0.535 mm, a thickness of about 0.25 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a third lens element having an object side surface of a radius of curvature of about 1.16 mm, an image side surface of a radius of curvature of about 0.536 mm, a thickness of about 0.445 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a fourth lens element having an object side surface of a radius of curvature of about 0.373 mm, an image side surface of a radius of curvature of about0.256 mm, a thickness of about 0.245 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a fifth lens element having an object side surface of a radius of curvature of about -0.639 mm, an image side surface of a radius of curvature of about 0.0257 mm, a thickness of about 0.15 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about 0.155 mm, an image side surface of a radius of curvature of about -1 .07 mm, a thickness of about 0.495 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a seventh lens element having an object side surface of a radius of curvature of about 0.481 mm, an image side surface of a radius of curvature of about -0.0328 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; an eighth lens element having an object side surface of a radius of curvature of about -0.272 mm, an image side surface of a radius of curvature of about -0.0448 mm, a thickness of about 0.30 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.64 mm, an image side surface of a radius of curvature of about -0.763 mm, a thickness of about 0.583 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a tenth lens element having an object side surface of a radius of curvature of about 0.686 mm, an image side surface of a radius of curvature of about 1 .04 mm, a thickness of about 0.373 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; an eleventh lens element having an object side surface of a radius of curvature of about 1 .05 mm, an image side surface of a radius of curvatureof about 0.849 mm, a thickness of about 0.46 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .52, and Abbe number of about 64.2, where the optical lens assembly comprises an aperture stop positioned between the sixth lens element and the seventh lens element, where the aperture stop is a planar element having a thickness of about 0.50 mm.

[0330] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, wavelength specific filter, or combination thereof, for multimodal microscopy.Example 5

[0331] FIG. 1 1 A illustrates an example layout diagram for a fifth embodiment of the optical lens assembly 2. The optical lens assembly 2 of the fifth embodiment comprises twelve lens elements 801 -812 which are sequentially arranged from object side to image side. The aperture stop 813 is located between the sixth lens element 806 and the seventh lens elements 807. The first six lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0332] The first lens element 801 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 802 has a refractive power. The image-side surface of the second lens element 802 includes a convex portion in the vicinity of the optical axis, and a concave portion in the vicinity of the periphery of the second lens element 802. The third lens element 803 has a refractive power. The object-side surface of the third lens element 803 is convex and the image-side surface of the thirdlens element 803 is concave in the vicinity of the axis. The fourth lens element 804 has a refractive power. The object-side surface of the fourth lens element 804 includes a convex portion in the vicinity of the optical axis and the image-side surface of the fourth lens element 804 is concave. The fifth lens element 805 has a refractive power. The object-side surface of the fifth lens element 805 is concave and the image-side surface of the fifth lens element 805 is either concave or plane surface. The sixth lens element 806 has a refractive power. The image-side surface of the sixth lens element 806 includes a convex portion in the vicinity of the periphery. The seventh lens element 807 has a positive refractive power. The object-side surface of the seventh lens element 807 is convex. The image-side surface of the seventh lens element 807 includes a convex. Both the object-side surface and the image-side surface of the seventh lens element 807 are aspherical. The eighth lens element 808 has a negative refractive power. The objectside surface of the eighth lens element 808 is convex. The image-side surface of the eighth lens element 808 is concave. Both the object-side surface and the image-side surface of the eighth lens element 808 are aspherical. The ninth lens element 809 has a positive refractive power. The object-side surface of the ninth lens element 809 is concave and the imageside surface of the ninth lens element 809 is convex. Both the object-side surface and the image-side surface of the ninth lens element 809 are aspherical. The tenth lens element 810 has a negative refractive power. The image-side surface of the tenth lens element 810 is concave. Both the object-side surface and the image-side surface of the tenth lens element 810 are aspheric. The inflection points are formed on the object-side surface and the image-side surface of the tenth lens element 810. The eleventh lens element 811 has a negative refractive power. The object-side surface of the eleventh lens element 81 1 is convex. The image-side surface of the eleventh lens element 81 1 is concave. Both the object-side surface and the image-side surface of the eleventh lens element 811 are aspheric. The inflection points are formed on the object-side surface and the image-sidesurface of the eleventh lens element 81 1 . The twelfth lens element 812 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The twelfth lens element 812 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 801 and the second lens element 802 or the first lens element 801 or the twelfth lens element 812 can act as a wavelength-specific filter in the optical lens assembly.

[0333] FIG. 1 1 B illustrates a distortion plot for the fifth embodiment of the optical lens assembly. Further, FIG. 1 1 C illustrates a MTF plot for the fifth embodiment of the optical lens assembly.

[0334] The optical lens assembly data for the fifth embodiment is shown in Table 6A and the aspheric data for the fifth embodiment of the optical lens assembly is shown in Table 6B. The optical lens assembly of the eighth embodiment has the following parameters.Magnifications = 1 .5X; Numerical aperture = 0.073; Focal Length = 2.07094; System length = 12.841 mm; HFOV = 13 degree highest HFOV; Depth of field = 47.95 microns; Airy radius = 4.964 micron.Table 6A: The optical lens assembly data for the fifth embodimentTable 6B: The aspheric data for the fifth embodiment of the optical lens assembly

[0335] The optical lens assembly of the fifth embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.3 mm, a refractive index of about 1 .515, and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -0.676 mm, an image side surface of a radius of curvature of about -0.6 mm, a thickness of about 0.842 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a third lens element having an object side surface of a radius of curvature of about 0.693 mm, an image side surface of a radius of curvature of about 1.1 mm, a thickness of about 0.494 mm, a refractive index of about 1.64, and Abbe number of about 23.3; a fourth lens element having an object side surface of a radius of curvature of about 0.512 mm, an image side surface of a radius of curvature of about 0.126 mm, a thickness of about 0.58 mm, a refractive index of about 1.54, and Abbe number of about 56.1 ; a fifth lens element having an object side surface of a radius of curvature of about -0.368 mm, an image side surface of a radius of curvature of about - 0.13 mm, a thickness of about 0.23 mm, a refractive index of about 1.64, and Abbe number of about 23.3; a sixth lens element having an object side surface of a radius of curvature of about -0.006 mm, an image side surface of a radius of curvature of about -0.593 mm, a thickness of about 0.643 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a seventh lens element having an object side surface of a radius of curvature of about 0.421 mm, an image side surface of a radius of curvature of about 0.02 mm, a thickness of about 0.644 mm, a refractive index of about 1 .54, and Abbe number of about 55.9;an eighth lens element having an object side surface of a radius of curvature of about 0.136 mm, an image side surface of a radius of curvature of about 0.371 mm, a thickness of about 0.432 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.0745 mm, an image side surface of a radius of curvature of about -0.217 mm, a thickness of about 1 .34 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a tenth lens element having an object side surface of a radius of curvature of about 0.16 mm, an image side surface of a radius of curvature of about 0.01 mm, a thickness of about 0.55 mm, a refractive index of about 1.54, and Abbe number of about 55.9; an eleventh lens element having an object side surface of a radius of curvature of about 0.31 mm, an image side surface of a radius of curvature of about 0.667 mm, a thickness of about 0.55 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.3 mm, a refractive index of about 1 .52, and Abbe number of about 64.2, where the optical lens assembly comprises an aperture stop positioned between the sixth lens element and the seventh lens element, where the aperture stop is a planar element having a thickness of about 0.5 mm.

[0336] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, wavelength specific filter, or combination thereof, for multimodal microscopy.Example 6

[0337] FIG. 12A illustrates an example layout diagram for a sixth embodiment of the optical lens assembly 2. The optical lens assembly 2 of the sixth embodiment comprises twelve lens elements 901 -912 which are sequentially arranged from object side to image side. The aperture stop 913 is located between the sixth lens element 906 and the seventh lens elements 907. The first six lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0338] The first lens element 901 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 902 has a refractive power. The image-side surface of the second lens element 902 includes a convex portion in the vicinity of the optical axis, and a concave portion in the vicinity of the periphery of the second lens element 902. The third lens element 903 has a refractive power. The object-side surface of the third lens element 903 is convex and the image-side surface of the third lens element 903 is concave in the vicinity of the axis. The fourth lens element 904 has a refractive power. The object-side surface of the fourth lens element 904 includes a convex portion in the vicinity of the optical axis and the image-side surface of the fourth lens element 904 is concave. The fifth lens element 905 has a negative refractive power. The object-side surface of the fifth lens element 905 is convex and the image-side surface of the fifth lens element 905 is either concave or plane surface. The sixth lens element 906 has a positive refractive power. The image-side surface of the sixth lens element 906 includes a convex portion in the vicinity of the periphery. The seventh lens element 907 has a positive refracting power. The object-side surface of the seventh lens element 907 is convex. The image-side surface of the seventh lens element 907 includes a convex. The eighth lens element 908 has a negative refracting power. The image-side surface of the eighth lens element 908 is concave. The ninth lens element 909 has a refracting power. The object-side surface of the ninth lenselement 909 is concave and the image-side surface of the ninth lens element 909 is convex. The tenth lens element 910 has a positive refracting power. The object-side surface of the tenth lens element 910 is concave near the vicinity of the optical axis. The image-side surface of the tenth lens element 910 is convex near the optical axis. The eleventh lens element 91 1 has a negative refracting power. The object-side surface of the eleventh lens element 91 1 comprises a concave portion in the vicinity of the optical axis. The image-side surface of the eleventh lens element 911 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the eleventh lens element 911 . The twelfth lens element 912 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The twelfth lens element 912 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 901 and the second lens element 902 or the first lens element 901 or the twelfth lens element 912 can act as a wavelength-specific filter in the optical lens assembly.

[0339] FIG. 12B illustrates a distortion plot for the sixth embodiment of the optical lens assembly. Further, FIG. 12C illustrates a MTF plot for the sixth embodiment of the optical lens assembly.

[0340] The optical lens assembly data for the sixth embodiment is shown in Table 7A and the aspheric data for the sixth embodiment of the optical lens assembly is shown in Table 7B. The optical lens assembly of the sixth embodiment has the following parameters.Magnifications = 2.6X ; Numerical aperture = 0.046; Focal Length = 1.1 18; System length = 8.89 mm; HFOV = 20 degrees; Depth of field= 75.3525; Airy radius = 8.89 microns.Table 7A: The optical lens assembly data for the sixth embodimentTable 7B: The aspheric data for the sixth embodiment of the optical lens assembly

[0341] The optical lens assembly of the sixth embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1.151 , 5 and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -1 .35 mm, an image side surface of a radius of curvature of about -1.2 mm, a thickness of about 0.412 mm, a refractive index of about 1.54, and Abbe number of about 56.1 ;10 a third lens element having an object side surface of a radius of curvature of about 1 .39 mm, an image side surface of a radius of curvature of about 2.2 mm, a thickness of about 0.247 mm, a refractive index of about 1.64, and Abbe number of about 23.3;a fourth lens element having an object side surface of a radius of curvature of about 1 .02 mm, an image side surface of a radius of curvature of about 0.252 mm, a thickness of about 0.29 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a fifth lens element having an object side surface of a radius of curvature of about -0.737 mm, an image side surface of a radius of curvature of about - 0.26 mm, a thickness of about 0.1 15 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a sixth lens element having an object side surface of a radius of curvature of about -0.0121 mm, an image side surface of a radius of curvature of about -1.19 mm, a thickness of about 0.322 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a seventh lens element having an object side surface of a radius of curvature of about 0.535 mm, an image side surface of a radius of curvature of about -0.0777 mm, a thickness of about 0.99 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eighth lens element having an object side surface of a radius of curvature of about -0.0129 mm, an image side surface of a radius of curvature of about 0.319 mm, a thickness of about 0.3 mm, a refractive index of about 1.63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.128 mm, an image side surface of a radius of curvature of about -0.186 mm, a thickness of about 0.49 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a tenth lens element having an object side surface of a radius of curvature of about -0.268 mm, an image side surface of a radius of curvature of about -0.579 mm, a thickness of about 0.89 mm, a refractive index of about 1 .54, and Abbe number of about 56.2;an eleventh lens element having an object side surface of a radius of curvature of about -0.267 mm, an image side surface of a radius of curvature of about 0.298 mm, a thickness of about 0.5 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 , where the optical lens assembly comprises an aperture stop positioned between the sixth lens element and the seventh lens element, where the aperture stop is a planar element having a thickness of about 0.5 mm.

[0342] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, wavelength specific filter, or combination thereof, for multimodal microscopy.Example 7

[0343] FIG. 13A illustrates an example layout diagram for a seventh embodiment of the optical lens assembly 2. The optical lens assembly 2 of the seventh embodiment comprises twelve lens elements 1001 -1012 which are sequentially arranged from object side to image side. The aperture stop 1013 is located between the sixth lens element 1006 and the seventh lens elements 1007. The first six lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0344] The first lens element 1001 is a protective layer or a wavelengthspecific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The second lens element 1002 has a refractive power. The object-side surface of the second lens element 1002 is aspherical and includes a convex portion in the vicinity of the optical axis, and a convex portion in the vicinity of the periphery of the second lenselement 1002. The third lens element 1003 has a positive refractive power and is aspherical. The object-side surface of the third lens element 1003 is concave and the image-side surface of the third lens element 1003 is convex in the vicinity of the optical axis. The fourth lens element 1004 has a positive refractive power. The object-side surface of the fourth lens element 1004 is convex and the image-side surface of the fourth lens element 1004 is concave. The fifth lens element 1005 has a negative refractive power. The object-side surface of the fifth lens element 1005 is concave. The sixth lens element 1006 has a positive refractive power. The image-side surface of the sixth lens element 1006 is convex. The first two lens elements from the object side are designed such that they manage the aberrations by other elements in the objective assembly. The seventh lens element 1007 has a positive refracting power. The object-side surface of the seventh lens element 1007 is convex. The image-side surface of the seventh lens element 1007 includes a convex. The eighth lens element 1008 has a negative refracting power. The object-side surface of the eighth lens element 1008 is convex, and image-side surface of the eighth lens element 1008 is concave. The ninth lens element 1009 has a positive refracting power. The object-side surface of the ninth lens element 1009 is a concave surface comprising a concave portion in the vicinity of the optical axis and the image-side surface of the ninth lens element 1009 is convex in the vicinity of the optical axis. The tenth lens element 1010 has a negative refracting power. The object-side surface of the tenth lens element 1010 is concave. The image-side surface of the tenth lens element 1010 is convex. The eleventh lens element 101 1 has a refracting power. The object-side surface of the eleventh lens element 101 1 comprises a convex portion in the vicinity of the optical axis and a concave portion in the vicinity of the periphery of the eleventh lens element. The image-side surface of the eleventh lens element 1011 comprises a concave portion in a vicinity of the optical axis and a convex portion in the vicinity of the periphery of the eleventh lens element 101 1. The twelfth lens element 1012 is at least oneof a wavelength-specific filter, a glass filter, or an IR filter. The twelfth lens element 1012 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 1001 and the second lens element 1002 or the first lens element 1001 or the twelfth lens element 1012 can act as a wavelength-specific filter in the optical lens assembly.

[0345] FIG. 13B illustrates a distortion plot for the seventh embodiment of the optical lens assembly. Further, FIG. 13C illustrates a MTF plot for the seventh embodiment of the optical lens assembly.

[0346] The optical lens assembly data for the seventh embodiment is shown in Table 8A and the aspheric data for the seventh embodiment of the optical lens assembly is shown in Table 8B. The optical lens assembly of the seventh embodiment has the following parameters. Magnifications = 2X; Numerical aperture = 0.044; Focal Length = 1.02515; System length = 7.42475 mm; HFOV = 20 degree highest HFOV; Depth of focus = 78.56 microns ; Airy radius = 8.401 microns.Table 8A: The optical lens assembly data for the seventh embodimentTable 8B: The aspheric data for the seventh embodiment of the optical lens assembly

[0347] The optical lens assembly of the seventh embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.075 mm, a refractive index of about 1 .52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -2.67 mm, an image side surface of a radius of curvature of about -1 .24 mm, a thickness of about 0.138 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a third lens element having an object side surface of a radius of curvature of about -0.04 mm, an image side surface of a radius of curvature of about -0.639 mm, a thickness of about 0.138 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a fourth lens element having an object side surface of a radius of curvature of about 0.868 mm, an image side surface of a radius of curvature of about 0.298 mm, a thickness of about 0.335 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a fifth lens element having an object side surface of a radius of curvature of about -1 .49 mm, an image side surface of a radius of curvature of about - 0.543 mm, a thickness of about 0.108 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about -0.08 mm, an image side surface of a radius of curvature of about -1 .68 mm, a thickness of about 0.161 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a seventh lens element having an object side surface of a radius of curvature of about 0.593 mm, an image side surface of a radius of curvature of about 0.006 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ;an eighth lens element having an object side surface of a radius of curvature of about 0.13 mm, an image side surface of a radius of curvature of about 0.368 mm, a thickness of about 0.23 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a ninth lens element having an object side surface of a radius of curvature of about -0.126 mm, an image side surface of a radius of curvature of about -0.512 mm, a thickness of about 0.58 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a tenth lens element having an object side surface of a radius of curvature of about -1.1 mm, an image side surface of a radius of curvature of about - 0.693 mm, a thickness of about 0.494 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; an eleventh lens element having an object side surface of a radius of curvature of about 0.6 mm, an image side surface of a radius of curvature of about 0.676 mm, a thickness of about 0.842 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; and a twelfth lens element having a planar object side surface, a planar image side surface, and a thickness of about 0.3 mm, a refractive index of about 1 .515, and Abbe number of about 63.96, where the optical lens assembly comprises an aperture stop positioned between the sixth lens element and the seventh lens element, where the aperture stop is a planar element having a thickness of about 0.259 mm.

[0348] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, or combination thereof, for multimodal microscopy.Example 8

[0349] FIG. 14A illustrates an example layout diagram for an eighth embodiment of the optical lens assembly 2. The optical lens assembly 2 of the eighth embodiment comprises thirteen lens elements 1201 -1213 which are sequentially arranged from object side to image side. The aperture stop 1214 is located between the seventh lens element 1207 and the eighth lens element 1208. The first seven lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0350] Both the first lens element 1201 and the second lens element 1202 are a protective layer or a wavelength-specific filter made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The third lens element 1203 has a refractive power. The object-side surface of the third lens element 1203 includes a concave portion in the vicinity of the optical axis, and a convex portion in the vicinity of the periphery of the third lens element 1203. The image side of the third lens element 1203 has a concave surface. The fourth lens element 1204 has a refractive power. The object-side surface of the fourth lens element 1204 is concave in the vicinity of the optical axis, and the image-side surface of the fourth lens element 1204 is concave. The fifth lens element 1205 has a positive refractive power. Both the image side surface and the object side surface of the fifth lens element 1205 are convex. The sixth lens element 1206 has a refractive power. The image-side surface of the sixth lens element 1206 is concave and the object side surface of the sixth lens element 1206 is one of plane, concave, or convex. The seventh lens element 1207 has a positive refractive power. The image-side surface of the seventh lens element 1207 is convex. In an example, the object-side surface of the seventh lens element 1207 is concave. In another example, the object-side surface of the seventh lens element 1207 is concave in the vicinity of the optical axis and convex in the peripheral vicinity. The eighth lens element 1208 has a positive refractive power. The object-side surface of the eighth lens element 1208 includes a convex portion in the vicinity of the periphery. In oneexample, the image-side surface of the eighth lens element 1208 is concave. In another example, the image-side surface of the eighth lens element 1208 is concave in the vicinity of the optical axis and either concave or plane in the peripheral vicinity. The ninth lens element 1209 has a refractive power. The object-side surface of the ninth lens element 1209 is either concave or plane. The tenth lens element 1210 has a positive refractive power. Both image side surface and the object side surface of the tenth lens element 1210 are convex. The eleventh lens element 1211 has a refractive power. The object-side surface of the eleventh lens element 1211 is concave, and the image-side surface of the eleventh lens element 121 1 is either concave or plane. The twelfth lens element 1212 has a refractive power. The object-side surface of the twelfth lens element 1212 is a concave surface. The image-side surface of the twelfth lens element 1212 is concave in the vicinity of the optical axis and convex in the peripheral vicinity. The thirteenth lens element 1213 is at least one of a wavelengthspecific filter, a glass filter, or an IR filter. The thirteenth lens element 1213 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength specific filter may be located between the first lens element 1201 and the second lens element 1202, or the first lens element 1201 or the thirteenth lens element 1213 can act as a wavelength-specific filter in the optical lens assembly.

[0351] FIG. 14B illustrates a distortion plot for the eighth embodiment of the optical lens assembly. Further, FIG. 14C illustrates a MTF plot for the eighth embodiment of the optical lens assembly.

[0352] The optical lens assembly data for the eighth embodiment is shown in Table 9A and the aspheric data for the eighth embodiment of the optical lens assembly is shown in Table 9B. The optical lens assembly of the eighth embodiment has the following parameters.Magnifications = 1 X; Numerical aperture = 0.189; Focal Length = 1.61224 mm; System length = 12.212 mm; HFOV = 70 degrees; Depth of field = 17.5 micron; Airy radius = 1 .97 micron.Table 9A: The optical lens assembly data for the eighth embodimentTable 9B: The aspheric data for the eighth embodiment of the optical lens assembly

[0353] The optical ens assembly of the eighth embodiment includes:a first lens element having a planar object side surface, a planar image side surface, a thickness of about 1 .2 mm, a refractive index of about 1 .517, and Abbe number of about 64.167; a second lens element having a planar object side surface, a planar image side surface, a thickness of about 0.5 mm, a refractive index of about 1 .517, and Abbe number of about 64.167; a third lens element having an object side surface of a radius of curvature of about -2.17 mm, an image side surface of a radius of curvature of about 14.7 mm, a thickness of about 0.2 mm, a refractive index of about 1.531 , and Abbe number of about 56.043; a fourth lens element having an object side surface of a radius of curvature of about -8.9 mm, an image side surface of a radius of curvature of about - 9.15 mm, a thickness of about 0.3 mm, a refractive index of aboutl .585, and Abbe number of about 29.909; a fifth lens element having an object side surface of a radius of curvature of about 3.5 mm, an image side surface of a radius of curvature of about -3.1 mm, a thickness of about 1.2 mm, a refractive index of about 1.531 , and Abbe number of about 56.043; a sixth lens element having a planar object side surface, an image side surface of a radius of curvature of about 4.2 mm, a thickness of about 0.2 mm, a refractive index of about 1 .634, and Abbe number of about 23.9; a seventh lens element having an object side surface of a radius of curvature of about -16.6 mm, an image side surface of a radius of curvature of about -2.83 mm, a thickness of about 0.5 mm, a refractive index of about 1 .531 , and Abbe number of about 56.044; an eighth lens element having an object side surface of a radius of curvature of about 3.25 mm, an image side surface of a radius of curvature of about 20 mm, a thickness of about 0.5 mm, a refractive index of about 1 .531 , and Abbe number of about 56.043;a ninth lens element having an object side surface of a radius of curvature of about -3.54 mm, a planar image side surface, a thickness of about 0.2 mm, a refractive index of about 1 .634, and Abbe number of about 23.9; a tenth lens element having an object side surface of a radius of curvature of about 3.1 mm, an image side surface of a radius of curvature of about - 3.5 mm, a thickness of about 1 .2 mm, a refractive index of about 1 .531 , and Abbe number of about 56.04; an eleventh lens element having an object side surface of a radius of curvature of about 13.5 mm, an image side surface of a radius of curvature of about -58.8 mm, a thickness of about 0.3 mm, a refractive index of about 1 .585, and Abbe number of about 29.91 ; a twelfth lens element having an object side surface of a radius of curvature of about -6 mm, an image side surface of a radius of curvature of about 2.4 mm, a thickness of about 0.2 mm, a refractive index of about 1.531 , and Abbe number of about 56.04; and a thirteenth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.25 mm, a refractive index of about 1 .5168, and Abbe number of about 64.16, where the optical lens assembly comprises an aperture stop positioned between the seventh lens element and the eighth lens element, where the aperture stop is a planar element having a thickness of about 0.1 mm.

[0354] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, a wavelength specific filter, or combination thereof, for multimodal microscopy.Example 9

[0355] FIG. 15A illustrates an example layout diagram for a ninth embodiment of the optical lens assembly 2. The optical lens assembly 2 ofthe ninth embodiment comprises eleven lens elements 1301 -131 1 which are sequentially arranged from object side to image side. The aperture stop 1312 is located between the seventh lens element 1307 and the eighth lens element 1308. The first seven lens elements can act as an objective assembly which can mount to any portable imaging system to enable the microscopic features for digital image capturing.

[0356] The first lens element 1301 and the second lens element 1302 are a protective layer or a wavelength-specific filter. The first lens element 1301 and the second lens element 1302 may be made of glass or plastic or polymer or composite materials having refractive index is greater than 1 . The third lens element 1303 has a refractive power. The image-side surface of the third lens element 1303 is plane and the object-side surface of the third lens element 1303 is concave. The fourth lens element 1304 has a positive refractive power. The image-side surface of the fourth lens element 1304 is convex. In an example, the object-side surface of the fourth lens element 1304 is convex. In another example, the object-side surface of the fourth lens element 1304 is convex in the vicinity of the optical axis and concave in the peripheral vicinity. The fifth lens element 1305 has a refractive power. The image-side surface of the fifth lens element 1305 is concave in the vicinity of the optical axis and convex in the peripheral vicinity. The object-side surface of the fifth lens element 1305 is convex. The sixth lens element 1306 has a refractive power. Both the image side surface and the object side surface of the sixth lens element 1306 are concave. The seventh lens element 1307 has a refractive power. The image-side surface of the seventh lens element 1307 is concave and the object-side surface of the seventh lens element 1207 is convex. The aperture stop 1312 is located between the seventh lens element 1307 and the eighth lens element 1308. The ninth lens element 1309 has a refractive power. The image-side surface of the ninth lens element 1309 is convex in the vicinity of the optical axis and concave in the peripheral vicinity. In an example, the object-side surface of the ninth lens element 1309 is concave.In another example, the object-side surface of the ninth lens element 1309 is concave in the vicinity of the optical axis and convex in the peripheral vicinity. The image-side surface of the tenth lens element 1310 is concave and the object-side surface of the tenth lens element 1310 is convex. The eleventh lens element 131 1 is at least one of a wavelength-specific filter, a glass filter, or an IR filter. The eleventh lens element 131 1 has a planar object side surface and a planar image side surface. For the filter field microscopic characteristics, an additional wavelength-specific filter may be located between the first lens element 1301 and second lens element 1302, or the first lens element 1301 or the eleventh lens element 131 1 can act as a wavelength-specific filter in the optical lens assembly. FIG. 15B illustrates a distortion plot for the ninth embodiment of the optical lens assembly. Further, FIG. 15C illustrates a MTF plot for the ninth embodiment of the optical lens assembly.

[0357] The optical lens assembly data for the ninth embodiment is shown in Table 10A and the aspheric data for the ninth embodiment of the optical lens assembly is shown in Table 10B. The optical lens assembly of the ninth embodiment has the following parameters.Magnifications = 4.5X; Numerical aperture = 0.438; Focal Length = 2.97mm; System length = 23.022 mm; HFOV = 70 degrees; Airy radius = 0.783 microns, Depth of field = 2.847 micron.Table 10A: The optical lens assembly data for the ninth embodimentTable 10B: The aspheric data for the ninth embodiment of the optical lens assembly

[0358] The optical lens assembly of the ninth embodiment includes: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 1 mm, a refractive index of about 1 .517, and Abbe number of about 63.96; a second lens element having a planar object side surface, a planar image side surface, a thickness of about 0.25 mm, a refractive index of about 1 .517, and Abbe number of about 63.96; a third lens element having an object side surface of a radius of curvature of about -2 mm, an image side surface of a radius of curvature of about 6.6 mm, a thickness of about 0.3 mm, a refractive index of about 1 .64, and Abbe number of about 23.9; a fourth lens element having an object side surface of a radius of curvature of about 3.39 mm, an image side surface of a radius of curvature of about - 1 .8 mm, a thickness of about 1 .8 mm, a refractive index of about 1 .517, and Abbe number of about 63.96; a fifth lens element having an object side surface of a radius of curvature of about 2.45 mm, an image side surface of a radius of curvature of about 5.67 mm, a thickness of about 1.3 mm, a refractive index of about 1.517, and Abbe number of about 63.96; a sixth lens element having an object side surface of a radius of curvature of about -2.5 mm, an image side surface of a radius of curvature of about2.6 mm, a thickness of about 0.3 mm, a refractive index of about 1 .643, and Abbe number of about 23.9; a seventh lens element having an object side surface of a radius of curvature of about 1 .27 mm, an image side surface of a radius of curvature of about 7.6 mm, a thickness of about 0.75 mm, a refractive index of about 1 .586, and Abbe number of about 30; an eighth lens element having an object side surface of a radius of curvature of about 2.82 mm, an image side surface of a radius of curvature of about 4.3 mm, a thickness of about 0.5 mm, a refractive index of about 1 .517, and Abbe number of about 63.96; a ninth lens element having an object side surface of a radius of curvature of about -1 .38 mm, an image side surface of a radius of curvature of about -2.16 mm, a thickness of about 0.3 mm, a refractive index of about 1.586, and Abbe number of about 30; a tenth lens element having an object side surface of a radius of curvature of about 2 mm, an image side surface of a radius of curvature of about 3.3 mm, a thickness of about 0.75 mm, a refractive index of about 1.535, and Abbe number of about 56; and an eleventh lens element having a planar object side surface, a planar image side surface, a thickness of about 0.5 mm, a refractive index of about 1 .517, and Abbe number of about 63.96, wherein the optical lens assembly comprises an aperture stop positioned between the seventh lens element and the eighth lens element, wherein the aperture stop is a planar element having a thickness of about 0.2 mm.

[0359] In an example implementation, the first and the last lens elements may be a polarizing filter, a color filter, a light guiding filter, a wavelength specific filter, or combination thereof, for multimodal microscopy.Table 12 tabulates the optical parameters of the nine example embodiments described herein.Table 12: The comparison table for all embodiments

[0360] In an example, a portable imaging system includes an optical microscopy device as described in the description herein. The portable imaging system is one of a smartphone, a portable computing device, a portable medical device, a portable microscope, and a portable analytical instrument.

[0361] The examples 1 to 9 describe and demonstrate embodiments within the scope of the present subject matter. The examples are provided herein solely for the purpose of illustration and is not to be construed as limitations of the present subject matter, as many variations thereof are possible without departing from spirit and scope.

[0362] The numeral values provided the present disclosure may include tolerance of about ±5%. The numeral values provided herein may have been rounded-off to the nearest decimal place for the sake of simplicity.

[0363] It may be noted that the serial numbering of the rows of the tables for each embodiment has one to one correspondence.FURTHER OPTICAL LENS ASSEMBLY EXAMPLESExample 10

[0364] The optical lens assembly of the embodiment of example 10 comprises ten lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fourth and fifth lens elements. First four lens elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnifications = 1 X; Numerical aperture = 0.072; Focal Length = 3.232 mm; System length = 12.67 mm; HFOV = 12.6 degree; Depth of field = 103.83 microns; Airy radius = 4.767 microns.Example 11

[0365] The optical lens assembly in the embodiment of example 1 1 comprises eight lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fourth and fifth lens elements. First four elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 7.8X; Numerical aperture = 0.015; Focal Length = 5.14 mm; System length = 56.87 mm; HFOV = 12.6 degree; Depth of field =68.55 microns; Airy radius = 23.25 micron.Example 12

[0366] The optical lens assembly in the embodiment of example 12 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth andsixth lens elements. First five elements can act as objective assembly. The optical assembly of this embodiment has Magnifications = 5X; Numerical aperture = 0.025; Focal Length = 0.6176 mm; System length = 7.65928 mm; HFOV = 30 degree; Airy radius = 14.74 microns; Depth of field = 39.73 micron.Example 13

[0367] The optical lens assembly in the embodiment of example 13 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 6X; Numerical aperture = 0.024; Focal Length = 0.677 mm; System length = 9.0321 mm; HFOV = 30 degree; Depth of field = 30.17 microns; Airy radius = 8.28 microns.Example 14

[0368] The optical lens assembly in the embodiment of example 14 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 2X; Numerical aperture = 0.050; Focal Length = 1.42983 mm; System length = 7.348 mm; HFOV = 25 degree; Depth of field = 58.23 microns; Airy radius = 7.142 microns.Example 15

[0369] The optical lens assembly in the embodiment of example 15 comprises twelve elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 3X;Numerical aperture = 0.059; Focal Length = 0.9843mm; System length = 6.63 mm; HFOV = 13 degree; Depth of field= 13.9 micron; Airy radius = 6.28 microns.Example 16

[0370] The optical lens assembly in embodiment of example 16 comprises twelve elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 1 X; Numerical aperture = 0.1 13; Focal Length = 2.12 mm; System length = 11.3 mm; HFOV = 13 degree; Depth of field= 48.396 micron; Airy radius = 3.25 micron.Example 17

[0371] The optical lens assembly in embodiment of example 17 comprises twelve elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly which can mount to any of the digital image capturing device to obtain the microscopy features of digital image capturing device. The optical assembly of this embodiment has: Magnifications = 4X; Numerical aperture = 0.027; Focal Length = 0.835534 mm; System length = 8.2546 mm; HFOV = 16 degree; Depth of field = 51 .58 microns; Airy radius = 13.44 microns.Example 18

[0372] The optical lens assembly in embodiment of example 18 comprises twelve elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 2X; Numerical aperture = 0.054; Focal Length = 1.0945 mm; System length = 7.03983 mm; HFOV = 16 degree; Depth of field = 52.62 micron; Airy radius = 6.786 micron.Example 19

[0373] The optical lens assembly in embodiment of example 19 comprises fourteen elements which are sequentially arranged from object side to image side. The aperture stop is located between the eighth and ninth lens elements. First eight elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 1.6X; Numerical aperture = 0.042; Focal Length = 1.362; System length = 10.03 mm; HFOV = 23 degree; Depth of field = 337.71 microns; Airy radius = 8.507 microns.Example 20

[0374] The optical lens assembly in embodiment of example 20 comprises fifteen elements which are sequentially arranged from object side to image side. The aperture stop is located between the eighth and ninth lens elements. First eight elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 2X; Numerical aperture = 0.044; Focal Length = 1.1388 mm; System length = 8.046 mm; HFOV = 23 degree; Depth of field = 78.899 microns; Airy radius = 8.205 microns.Example 21

[0375] The optical lens assembly in embodiment of example 21 comprises twelve elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical assembly of this embodiment has: Magnifications = 1 X; Numerical aperture = 0.066; Focal Length = 1 .53861 mm; System length = 9.39775 mm; HFOV = 20 degree; Depth of field = 33.90 microns; Airy radius = 5.56 microns.Example 22

[0376] The optical lens assembly in embodiment of example 22 comprises ten lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fourth and fifth lens elements. First four lens elements can act as objective assembly. Theoptical lens assembly of this embodiment has: Magnification = 2X; Numerical aperture = 0.035; Focal Length = 3.35 mm; System length = 17.90 mm; HFOV = 12.6 degree; Depth of field =121 .38 micron; Airy radius = 10.31 micron.Example 23

[0377] The optical lens assembly in embodiment of example 23 comprises ten lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five elements can act as objective assembly. The optical assembly of this embodiment has: Magnification = 2X; Numerical aperture = 0.046; Focal Length = 1.49093 mm; System length = 8.7613 mm; HFOV = 20-degree highest HFOV; Depth of field = 69.99 micron; Airy radius = 7.828 micron.Example 24

[0378] The optical lens assembly in embodiment of example 24 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 1 X; Numerical aperture = 0.046; Focal Length = 2.05; System length = 12.211 mm; HFOV = 20 degree; Depth of field=278.24 micron; Airy radius =7.803 micron.Example 25

[0379] The optical lens assembly in embodiment of example 25 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 3X; Numerical aperture = 0.025; Focal Length = 1.02207; System length = 8.44428 mm; HFOV = 25 degree; Depth of field =109.85 micron; Airy radius = 14.71 micron.Example 26

[0380] The optical lens assembly in embodiment of example 26 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 2X; Numerical aperture = 0.041 ; Focal Length = 1 .29847 mm; System length = 7.63525 mm; HFOV = 20 degree; Airy radius = 8.818 micron; Depth of field = 88.83 micron having a thickness of about 0.2 mm.Example 27

[0381] The optical lens assembly in embodiment of example 27 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 1 X; Numerical aperture = 0.103; Focal Length = 1.45725; System length = 8.33162 mm; HFOV = 21 degree; Depth of field = 55.51 micron; Airy radius =3.487 micron.Example 28

[0382] The optical lens assembly in embodiment of example 28 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the sixth and seventh lens elements. First six elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 3X; Numerical aperture = 0.047; Focal Length = 0.8999245 mm; System length = 6.89459 mm; HFOV = 25 degree; Airy radius = 7.673 micron; Depth of field = 28.8898 micron.Example 29

[0383] The optical lens assembly in embodiment of example 29 comprises fifteen lens elements which are sequentially arranged from object side toimage side. The aperture stop is located between the seventh and eighth lens elements. First seven elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 1 X; Numerical aperture = 0.068; Focal Length = 1 .0786; WFNO = 7.501 ; System length = 8.189 mm; HFOV = 18 degree; HFOV; Depth of focus =132.1 1 micron; Airy radius = 5.378 micron.Example 30

[0384] The optical lens assembly in embodiment of example 30 comprises sixteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the eighth and ninth lens elements. First eight elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 1 X; Numerical aperture = 0.176; Focal Length = 1 .35439; WFNO = 8.36712 System length = 10.00917 mm; HFOV = 38.35 degree; Depth of field = 164.5377 micron; Airy radius =5.998 micron.Example 31

[0385] The optical lens assembly in embodiment of example 31 comprises fourteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the eighth and ninth lens elements. First eight elements can act as objective assembly. The optical lens assembly of this embodiment has: Magnification = 2X; Numerical aperture = 0.052; Focal Length = 1.244 mm; WFNO = 9.74505; System length = 9.58348 mm; HFOV = 36 degree; Airy radius = 6.985 micron; Depth of field = 55.68 micron.Example 32

[0386] The optical lens assembly in embodiment of example 32 comprises fifteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the eighth and ninth lens elements. First eight elements can act as objective assembly. The opticallens assembly of this embodiment has: Magnification = 1 X, Numerical aperture = 0.072, Focal length = 1 .34814, WFNO = 7.06268, System length: 8.8425 mm, HFOV: 38.35 degree, Airy Radius: 5.063 micron, Depth of field: 1 17.12 micron.Example 33

[0387] The optical lens assembly in embodiment of example 33 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters: Magnifications = 3X; Numerical aperture = 0.057; Focal Length = 0.9473 mm; System length = 6.559 mm; HFOV = 30 degree; Depth of field = 13.124 micron; Airy radius = 6.413 micron.Example 34

[0388] The optical lens assembly in embodiment of example 34 comprises thirteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters: Magnifications = 2X; Numerical aperture = 0.055; Focal Length = 1.2369 mm; System length = 9.48 mm; HFOV = 33 degree; Depth of field = 50.917 microns; Airy radius = 6.673 microns.Example 35

[0389] The optical lens assembly in embodiment of example 35 comprises twelve lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as objective lens group. The optical lens assembly of this embodiment has the following parameters: Magnifications = 4X; Numerical aperture = 0.038; Focal Length = 0.721 1 ;System length = 6.81276 mm; HFOV = 30 degree; Depth of field = 26.76 micron; Airy radius = 9.676 micron.Example 36

[0390] The optical lens assembly in embodiment of example 36 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnifications = 2X; Numerical aperture = 0.104; Focal Length = 0.9718 mm; System length = 5.51 1 mm; HFOV = 20 degree; Airy radius = 3.655 microns; Depth of field = 3.003 microns.Example 37

[0391] The optical lens assembly in embodiment of example 37 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnifications = 1X; Numerical aperture = 0.1 16; Focal Length = 0.5230 mm; System length = 2.89380 mm; HFOV = 30-degree; Airy radius = 3.14 microns; Depth of field = 3.6077 microns.Example 38

[0392] The optical lens assembly in embodiment of example 38 comprises ten lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnifications = 1 X; Numerical aperture =0.120; Focal Length = 1.141 1 1 mm; System length = 5.86618 mm; HFOV = 30 degree; Depth of field = 41 .4650 micron; Airy radius = 3.012 micron.Example 39

[0393] The optical lens assembly in embodiment of example 39 comprises thirteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnifications = 2X; Numerical aperture = 0.061 ; Focal Length = 1.1316 mm; System length = 8.38276 mm; HFOV = 30 degree; Depth of field = 40.42 micron ; Airy radius = 5.946 micron.Example 40

[0394] The optical lens assembly in embodiment of example 40 comprises ten lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has following parameters. Magnification = 2X, Numerical aperture = 0.057, Focal length = 1.89982 mm ; System length: 9.37121 mm, HFOV: 20 degree, Airy Radius: 6.328 microns, Depth of field : 45.74 micron.Example 41

[0395] The optical lens assembly in embodiment of example 41 comprises nine lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnification = 2X, Numerical aperture = 0.070, Focal length = 2.31407 mm , System length: 10.019 mm, HFOV: 18 degree, Airy Radius: 5.229 microns, Depth of field: 31 .23 micron.Example 42

[0396] The optical lens assembly in embodiment of example 42 comprises thirteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters: Magnification = 2X, Numerical aperture = 0.033, Focal length = 1 .24722 mm, System length: 9.35024 mm, HFOV: 20 degree, Airy Radius: 10.78 microns, Depth of field: 132.64 micron.Example 43

[0397] The optical lens assembly in embodiment of example 43 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters: Magnification = 3X, Numerical aperture = 0.026, Focal length = 2.19641 mm, System length: 10.657 mm, HFOV: 20 degree, Airy Radius: 13.57 microns, Depth of field: 93.495 micron.Example 44

[0398] The optical lens assembly in embodiment of example 44 comprises eleven lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnification = 2X, Numerical aperture = 0.067, Focal length = 1 .04835 mm, System length: 5.49070 mm, HFOV: 10 degree, Airy Radius: 5.465 microns, Depth of field: 34.1 1 micron.Example 45

[0399] The optical lens assembly in embodiment of example 45 comprises twelve lens elements which are sequentially arranged from object side toimage side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnification = 4X, Numerical aperture = 0.021 , Focal length = 0.7333547 mm ; System length = 0.891 mm, HFOV = 18-degree, Airy Radius = 16.82 microns, Depth of field = 80.78 micron.Example 46

[0400] The optical lens assembly in embodiment of example 46 comprises thirteen lens elements which are sequentially arranged from object side to image side. The aperture stop is located between the fifth and sixth lens elements. First five lens elements can act as an objective lens group. The optical lens assembly of this embodiment has the following parameters. Magnification = 1 X; Numerical Aperture = 0.062; Focal Length = 1.94329 mm; System Length = 1 1 .595 mm; Airy radius = 5.814; micron; HFOV = 17 degree ; Depth of Field. = 154.483.

[0401] FIG. 16 illustrates a flow chart depicting a method 1600 for processing an image of an object, in accordance with an example implementation of the present subject matter. The method 1600 is described with reference to the microscopy imaging system 222 of FIG. 2A. The object may include micron sized objects, such as objects having size as small as 0.044 micron. For example, the object may include but not limited to a blood sample, dust particles, pollen, water droplets, fungus, spider web silk, smoke particles, bacteria, and viruses.

[0402] At block 1602, the method includes capturing an image of an object. The image may be captured using the image sensor 106 of FIG. 1 A. through the optical lens assembly 104 of FIG. 1 A. In an example, the optical assembly may include eight to sixteen lens elements. In another example, the image sensor may have a pixel pitch in a range of about 0.2 micron toabout 6 micron. In an example, the image sensor may be a camera of a mobile phone.

[0403] At block 1604, the captured image may be processed. In one example implementation, the captured image may be processed by converting the image to a digitized form. In an example implementation, the digitized form is an image made of pixels. The digitized form of the image may be processed by the processing unit 228 of FIG. 2A.

[0404] At block 1606, a unique or multiple characteristic feature of the object may be detected. In one example implementation, the unique or multiple characteristic feature may be detected by analyzing the digitized form of the image using an artificial intelligence and machine learning based model. The unique or multiple characteristic feature may be a feature, for example white blood cells in a blood sample if the object is the blood sample. In an example implementation, the artificial intelligence and machine learning based model may use the digitized form of the image provided by the processing unit 228 of FIG. 2A for recognizing parameters in the digitized form of the image. The recognized parameters may be indicative of the unique or multiple characteristic feature of the object. For example, the recognized parameters may include a number of white blood cells count in the blood sample. In another example implementation, the artificial intelligence and machine learning based model may be trained based on the detected characteristic features of the object. For example, the number of white blood cells count is fed to the artificial intelligence and machine learning based model. Based on such trained artificial intelligence and machine learning based models, helpful applications for image recognition for example, fever diagnosis may be created.

[0405] At block 1608, the detected characteristic feature may be compared with pre-stored dataset. For the comparison, the detected characteristic feature is mapped with a relevant parameter, of the object, present in the pre-stored dataset. The pre-stored dataset may be a dataset stored in the database 224 of FIG. 2A that include different parameters of an object. Forexample, the different parameters may include a number of red blood cells count for being qualified as a healthy blood sample.

[0406] At block 1610, based on the comparison, a report may be generated to identify a category of the detected characteristic feature of the object with or without difference or similarity in the detected characteristic feature. In an example, if the mapping indicates low count of the red blood cells, then the identified category may be anaemia. In another example, if the mapping indicates low count of the white blood cells, then the identified category may be Leukopenia. The identified category of the unique or multiple characteristic feature may be displayed in the form of one of an image, a text, and a report. In an example implementation, the generated report is transmitted via a transmitter to another device. The transmitter may be coupled to the processing unit 228 of FIG. 2A. In an example implementation, data related to the category of the unique or multiple characteristic feature of the image may be transferred to a cloud database. For example, the cloud database may be further referred to map the detected characteristic feature at block 1608. In another example implementation, data related to the category of the unique or multiple characteristic feature of the image may be transferred to one of an inter device, an intra device, and a cloud device using a wired or a wireless communication channel. For example, one of the inter device, the intra device, and the cloud device using a wired or a wireless communication channel may be further referred to map the detected characteristic feature at block 1608.

[0407] The above method 1600 may be utilized for automated detection, quantification and analysis of objects and further quick interpretation of various sectors such as healthcare, water, food, education, research, microbiology, biotechnology, botany, and agriculture etc.

[0408] Furthermore, the above-mentioned method 1600 may be implemented in a suitable hardware, computer-readable instructions, or combination thereof. The steps of such methods may be performed byeither a system under the instruction of machine executable instructions stored on a non-transitory computer readable medium or by dedicated hardware circuits, microcontrollers, or logic circuits. Herein, some examples are also intended to cover non-transitory computer readable medium, for example, digital data storage media, which are computer readable and encode computer-executable instructions, where the instructions perform some or all the steps of the above-mentioned methods.

[0409] Case studies of applications of the method 1600 and the microscopy imaging system 222 in various sectors such as healthcare, water, food, education, research, microbiology, biotechnology, botany, and agriculture etc. are detailed as follow:Case study for detection of adulteration

[0410] The present example describes the application of the method 1600 and the microscopy imaging system 222 in adulteration detection within herbal products and further mapping of adulteration data as per the duration, date wise, batch-wise, etc. to respective users using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database to identify the adulteration in the herbal product.

[0411] In operation, the sample may be collected from the herbal product and the collected sample may be used for a microscopic slide preparation. The microscopic slide may be prepared by making a suspension of the sample in water. Further, an appropriate staining process may be used for the detection of adulterated parts or herbs. The suspension of the sample is placed on the objective slide, and it is used for microscopic observation by capturing an image of the sample using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the sample is further sent to the processing unit 228 of the microscopy imaging system 222 for the subsequent image processing. In an example, the processing unit 228 may be a cloud processing unit or astandalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated adulteration detection using artificial intelligence and machine learning based model, and Adulteration prediction from the database 224, and subsequently stored information is used to determine the image. The image processing using the microscopy imaging system 222 provides the possible detection and prediction of the herbal adulteration present on the object or the sample slide. This analyzed data about the herbal adulteration can be possibly used in three ways. Firstly, it can be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities. Secondly, the analyzed data can be shared over the cloud for adulteration prediction and mapping as per various parameters like a batch sample, date, time, duration, etc. The obtained analysis report of adulteration prediction can be shared on screen in the form of images, text, reports, etc. on the screen. This entire data and information can further be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities. Thirdly, the obtained analysis report of adulteration prediction can be shared on the display unit in the form of images, text, reports, etc. on the screen. The data can further be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities.Case study of water contamination detection and alert system

[0412] The present example describes the application of the method 1600 and the microscopy imaging system 222 in water contamination detection and alert system for its use. In the present example, the high-quality image may be captured by the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing usingthe artificial intelligence and machine learning based model and the cloud database may be used for the identification of water contamination.

[0413] In operation, a water sample may pass through the sample preparation such as concentrating of the sample through filtration, concentration, staining and non-staining process followed by microscopic slide preparation. The prepared sample slide may be placed under the compact optical microscopy device 102 of the portable imaging system 100 where the high-quality microscopic image is captured. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222 for the subsequent image processing. In an example, the processing unit 228 may be a cloud or a standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated microorganism detection using artificial intelligence and machine learning based model, and contamination prediction from the database 224, and subsequently stored information is used to determine the image. The image processing using the microscopy imaging system 222 provides the possible detection and prediction of water contamination. This analyzed data about the detection of microbial contamination in water can be possibly used in three ways. Firstly, it can be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, Government or Private bodies. Secondly, it can be shared over the cloud for detection of microbial contamination in water and mapping as per various parameters like location, batch sample, date, time, duration, etc. Thirdly, the obtained analysis report of food contamination prediction can be shared on screen in the form of images, text, reports, etc. on the screen. If the data is used in the second and third manner then after the steps are completed, they will be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels.Case study for detection of crop disease

[0414] The present example describes the application of the method 1600 and the microscopy imaging system 222 in crop disease detection within plants and mapping of diseases in agriculture plants using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for the prediction of diseases in the crop.

[0415] In operation, the sample may be collected from the plant and the collected sample may be used for a microscopic slide preparation. The microscopic slide may be prepared without staining. In an example, an appropriate staining process may be used to detect the microorganism responsible for diseases. The microscopic slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated microorganism detection using artificial intelligence and machine learning, and disease prediction from the database 224, and subsequently stored information is performed on the image. The image processing using the microscopy imaging system 222 provides the possible detection and prediction of crop diseases. This analyzed data about crop diseases may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to experts for possible remedies to farmers. The obtained analysis report of adulteration prediction may be shared on screen in the form of images, text, reports, etc. on the screen. This entire data and information may further be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities.Case study for cell viability and total cell count

[0416] The present example describes the application of the method 1600 and the microscopy imaging system 222 in cell viability and total cell count using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for the total cell count and viable count of cells in the captured image.

[0417] In operation, the sample may be collected. In an example, the collected sample may be pre-processed using staining process for the slide preparation. In another example, the collected sample may be pre- processed using non-staining process for the slide preparation. The slide may be prepared for detection of specific microbe without staining or an appropriate staining process, for total microbial count of specific microorganism and for the viability of cells based on staining of cells. The sample slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated counting of stained, unstained, and total microbial cell count using artificial intelligence and machine learning. The obtained analysis report for cell viability counting may be shared on screen in the form of images, text, reports, etc. on the screen. In an example, the obtained analysis report of adulteration prediction may be shared on screen in the form of images, text, reports, etc. on the screen. This entire data and information may further be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities. The data for viability count may be mapped as per the data in software or the cloud as per various parameterslike a batch sample, date, time, duration, etc using the compact optical microscopy device 102 of the portable imaging system 100. These final mapping data may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities.Case study for automated object detection and interactive smart learning of micro world

[0418] The present example describes the application of the method 1600 and the microscopy imaging system 222 in automated object detection and interactive smart learning of micro world using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for the automated object detection in the captured image and image display relevant to object such as text, diagram, animation, video, and information etc.

[0419] In operation, the image may be captured directly by inbuilt microscopic feature of smartphone or digital device. In an example, the sample slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated object feature extraction using artificial intelligence and machine learning, and object identification from database 224. The obtained analysis report of images may be shared on screen in the form of images, text, reports, etc. on the screen. The data further may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels.Case study for detection of Food contamination and alert system

[0420] The present example describes the application of the method 1600 and the microscopy imaging system 222 in food contamination detection and alert for contamination in food using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for the identification of food contamination.

[0421] In operation, the sample may be collected from the food and the collected sample passes through the sample preparation such as staining and non-staining process followed by microscopic slide preparation. The prepared sample slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated microorganism detection using artificial intelligence and machine learning, and contamination prediction from the database 224, and subsequently stored information is performed on the image. The image processing using the microscopy imaging system 222 provides the possible detection and prediction of food contamination. This analyzed data about the detection of microbial contamination in food may be possibly used in three ways. Firstly, it may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, government or private bodies. Secondly, it may be shared over the cloud for detection of microbial contamination in food and mapping as per various parameters like location, batch sample, date, time, duration, etc. Thirdly, the obtained analysis report of food contamination prediction may be shared on screen in the form of images, text, reports, etc. on the screen. If the data is used in the second and third manner then after the steps are completed, they willbe stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels.Case study for rapid detection of disease

[0422] The present example describes the application of the method 1600 and the microscopy imaging system 222 in rapid detection of a pathology condition or disease using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database. The portable imaging system 100 reduces the time and cost of diagnosis and is easily accessible for rural areas or areas with a lack of resources.

[0423] In an implementation, the sample may be collected. In an example, the collected sample may be pre-processed using staining. In another example, the collected sample may be pre-processed using non-staining. The microscopic slide may be prepared to detect the pathological condition, pathogen, pathology parameters such as cell count, tissue, abnormal conditions and responsible for diseases. The prepared sample slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as Pre-processing, automated feature extraction using artificial intelligence and machine learning and qualitative and quantitative analysis of cells, tissue, blood, urine, stool, a biopsy sample, saliva, and pathogens from the database 224. The pathology condition results and remedies may directly display on the screen in form of reports or data, which can be directly used by local doctors or users. The analysis report may be shared with the doctor based via cloud storage or via database storage and based on that the doctor will suggest remedies.In another path, the data after image processing using the microscopy imaging system 222 is either stored, shared, or transferred to inter, intra, and cloud devices over wired or wireless communication channels to the pathologist or is used to predict the specific disease or pathological condition and further recommendation share to the doctor for the taking decision of the diseases or possible treatment. The obtained analysis report of images may be shared on screen in the form of an image, text, reports, etc. on the screen. The data further may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels.Case study for microbial growth rate and contamination detection

[0424] The present example describes the application of the method 1600 and the microscopy imaging system 222 in microbial growth rate and contamination detection using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for the microbial growth rate measurement and contamination detection.

[0425] In operation, the sample may be collected from a bioreactor and the collected sample passes through the sample preparation such as staining and non-staining process followed by microscopic slide preparation. The prepared microscopic slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing, automated microorganism detection and total cell counting using artificial intelligence and machine learning, and contamination prediction from the database 224, and subsequently storedinformation is performed on the image. This analysed data about the microbial type, total count, growth rate and contamination detection may be possibly used in three ways. Firstly, it may be stored, shared, transferred to inter, intra, cloud devices over wired or wireless communication channels to be used by the users. Secondly, it may be shared over cloud for microbial type, total count, growth rate and contamination as per various parameters like batch sample, date, time, duration, etc. Thirdly, the obtained analysis report may be shared on screen in the form of images, text, reports, etc. on the screen. This data may further be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user, agency, or authorities.Case study for size distribution analysis

[0426] The present example describes the application of the method 1600 and the microscopy imaging system 222 in size distribution analysis using the compact optical microscopy device 102 of the portable imaging system 100 and subsequently the image processing using the artificial intelligence and machine learning based model and the cloud database for size distribution analysis.

[0427] In operation, the sample may be collected and the collected sample is prepared according to the powder / emulsion and suspension required for passing the sample through the microscope. The prepared sample slide may be placed under the microscopic camera of the smartphone or digital device for capturing the high-quality microscopic image using the compact optical microscopy device 102 of the portable imaging system 100. The captured microscopic image of the slide is further sent to the processing unit 228 of the microscopy imaging system 222. In an example, the processing unit 228 may be a cloud or standalone processing unit. The processing unit 228 may perform stepwise analysis such as pre-processing and counting of different sizes of particles / droplets on the received images. This analyzed data about information about size distribution, in the form of graphs, numbers, diagrams, tables, etc. may be possibly used in three ways. Firstly,it may be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the users. Secondly, it may be shared over the cloud for size distribution, in the form of graphs, numbers, diagrams, tables, etc. as per various parameters like the batch sample, date, time, duration, etc. Thirdly, the obtained analysis report may be shared on screen in the form of images, text, reports, etc. on the screen. If the data is used in the second and third manner then after the steps are completed, they will be stored, shared, and transferred to inter, intra, and cloud devices over wired or wireless communication channels to be used by the user.

[0428] Although examples for the present disclosure have been described in language specific to structural features and / or methods, it is to be understood that the appended claims are not necessarily limited to the specific features or methods described. Rather, the specific features and methods are disclosed and explained as examples of the present disclosure.

Claims

I / We Claim:1 . A microscopy imaging system (222) comprising: a compact optical microscopy device (102) comprising: an optical lens assembly (104) with eight to sixteen lens elements, wherein the optical lens assembly (104) has: an optical magnification in a range of about 1X to about 7.8X; an airy radius in a range of about 0.783 micron to about 23.25 micron; a depth of field in a range of about 2.847 micron to about 338 micron; a numerical aperture in a range of about 0.015 to about 0.438; a half field of view in a range of about 13 degrees to about 70 degrees; and a length in a range of about 5.51 1 millimeter (mm) to about 57 mm; and an image sensor (106) to capture an image of an object through the optical lens assembly (104), wherein the image sensor (106) has a pixel pitch in a range of about 0.2 micron to about 6 micron, and a processing unit (228) communicatively coupled to the compact optical microscopy device (102), wherein the processing unit (228) is disposed within the compact optical microscopy device (102) or outside the compact optical microscopy device (102), wherein the processing unit (228) is to:process the captured image by converting the image to a digitized form; detect a unique or multiple characteristic feature of the object by analyzing the digitized form of the image using an artificial intelligence and machine learning based model; compare the detected characteristic feature with pre-stored dataset, wherein for the comparison, the detected characteristic feature is mapped with a relevant parameter, of the object, present in the pre-stored dataset; and generate a report based on the comparison, wherein the report is to identify a category of the detected characteristic feature of the object with or without difference or similarity in the detected characteristic feature.

2. The microscopy imaging system (222) as claimed in claim 1 , wherein the processing unit (228) is to transfer data related to the category of the unique or multiple characteristic feature of the image to a cloud database.

3. The microscopy imaging system (222) as claimed in claim 1 , wherein the processing unit (228) is to transfer data related to the category of the unique or multiple characteristic feature of the image to one of an inter device, an intra device, and a cloud device using a wired or a wireless communication channel.

4. The microscopy imaging system (222) as claimed in claim 1 , wherein the microscopy imaging system (222) comprises a display unit, connected to the processing unit (228), to display the identified category of the unique or multiple characteristic feature in the form of one of an image, a text, and a report.

5. The microscopy imaging system (222) as claimed in claim 4, wherein the processing unit (228) is to share the identified category of the unique or multiple characteristic feature displayed by the display unit to one of an interdevice, an intra device, and a cloud device using a wired or a wireless communication channel.

6. The microscopy imaging system (222) as claimed in claim 1 , wherein the microscopy imaging system (222) is used to capture and process the image of the sample related to fields including one of adulteration detection, agriculture, cell viability counting, education, food contamination, healthcare, microbial growth rate, size distribution analysis, and water contamination.

7. The microscopy imaging system (222) as claimed in claim 1 , wherein the microscopy imaging system (222) comprises a transmitter coupled to the processing unit (228), wherein the processing unit (228) is to transmit the generated report via the transmitter to another device.

8. The microscopy imaging system (222) as claimed in claim 1 , wherein the compact optical microscopy device (102) comprises a focusing unit having a barrel, wherein the optical lens assembly (104) is disposed within the barrel of the focusing unit, and wherein the focusing unit is to operate the optical lens assembly (104) for focusing while imaging the object.

9. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has nine or eleven or twelve or thirteen or fourteen or fifteen lens elements.

10. The microscopy imaging system (222) as claimed in claim 9, wherein the optical lens assembly (104) has a first lens element configured as a wavelength-specific optical filter.1 1 . The microscopy imaging system (222) as claimed in claim 9, wherein the optical lens assembly (104) has a last lens element configured as a wavelength-specific optical filter.

12. The microscopy imaging system (222) as claimed in claim 1 , wherein the compact optical microscopy device (102) has a wavelength-specific optical filter positioned:between a first lens element and a second lens element of the optical lens assembly (104); or in front of the first lens element; or between the image sensor (106) and a last lens element of the optical lens assembly (104); or between an adjacent pair of lens elements of the optical lens assembly (104).

13. The microscopy imaging system (222) as claimed in claim 1 , wherein the compact optical microscopy device (102) comprises a first light source, wherein the first light source comprises at least one light emitting diode (LED) placed at a circumferential boundary of the optical lens assembly (104) to emit light such that the emitted light is incident from a first side on a sample held within a sample holder unit, when the sample holder unit is placed against the optical lens assembly (104).

14. The microscopy imaging system (222) as claimed in claim 13, wherein the first light source comprises a plurality of LED placed at a circumferential boundary of the optical lens assembly (104) to emit light in a form of a ring such that the emitted light is incident on a sample held within a sample holder unit, when the sample holder unit is placed over the optical lens assembly (104).

15. The microscopy imaging system (222) as claimed in claim 13, wherein the at least one LED is a white LED.

16. The microscopy imaging system (222) as claimed in claim 13, wherein the at least one LED is a wavelength-specific LED emitting light of a wavelength in a range of about 200 nm to about 700 nm.

17. The microscopy imaging system (222) as claimed in claim 13, wherein the at least one LED is placed such that the emitted light is incident on the sample in the sample holder unit at an angle in a range of about 5° to about 90° with respect to a planar surface of the sample holder unit.

18. The microscopy imaging system (222) as claimed in claim 13, wherein the at least one LED is placed such that the emitted light is incident on the sample in the sample holder unit at an angle in a range of about 48° to about 49° with respect to a planar surface of the sample holder unit.

19. The microscopy imaging system (222) as claimed in claim 13, wherein the at least one LED is placed such that the emitted light is incident on the sample in the sample holder unit at an angle of about 48.5° with respect to a planar surface of the sample holder unit.

20. The microscopy imaging system (222) as claimed in claim 1 , wherein the compact optical microscopy device (102) comprises a polarized filter disposed in front of a first lens element of the optical lens assembly (104).

21. The microscopy imaging system (222) as claimed in claim 1 , wherein compact optical microscopy device (102) comprises a protective glass cover disposed in front of a first lens element of the optical lens assembly (104).

22. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 2X, a numerical aperture of about 0.104, a length of about 5.51 1 mm, a half field of view of about 20 degree, a depth of field of about 12.015 micron, and an airy radius of about 3.655 micron, wherein the optical lens assembly (104) comprises eleven lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.105 mm, a refractive index of about 1 .517, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.284 mm, an image side surface of a radius of curvature of about -0.409 mm, a thickness of about 0.154 mm, a refractive index of about 1 .535, and Abbe number of about 56.0; a third lens element having an object side surface of a radius of curvature of about 1 .04 mm, an image side surface of a radius of curvatureof about -2.41 mm, a thickness of about 0.293 mm, a refractive index of about 1 .544, and Abbe number of about 56.1 ; a fourth lens element having an object side surface of a radius of curvature of about 0.837 mm, an image side surface of a radius of curvature of about 0.365 mm, a thickness of about 0.145 mm, a refractive index of about 1 .635, and Abbe number of about 23.9; a fifth lens element having an object side surface of a radius of curvature of about 1 .03 mm, an image side surface of a radius of curvature of about -0.75 mm, a thickness of about 0.237 mm, a refractive index of about 1 .544, and Abbe number of about 56.1 ; a sixth lens element having an object side surface of a radius of curvature of about 3.2 mm, an image side surface of a radius of curvature of about 7.87 mm, a thickness of about 0.38 mm, a refractive index of about 1 .535, and Abbe number of about 55.712; a seventh lens element having an object side surface of a radius of curvature of about -10.00 mm, an image side surface of a radius of curvature of about -1.10 mm, a thickness of about 0.471 mm, a refractive index of about 1 .535, and Abbe number of about 55.712; an eighth lens element having an object side surface of a radius of curvature of about -0.471 mm, an image side surface of a radius of curvature of about -0.772 mm, a thickness of about 0.22 mm, a refractive index of about 1 .638, and Abbe number of about 23.256; a ninth lens element having an object side surface of a radius of curvature of about 4.58 mm, an image side surface of a radius of curvature of about -0.878 mm, a thickness of about 0.694 mm, a refractive index of about 1 .535, and Abbe number of about 55.712; a tenth lens element having an object side surface of a radius of curvature of about 2.83 mm, an image side surface of a radius of curvatureof about 0.775 mm, a thickness of about 0.482 mm, a refractive index of about 1 .638, and Abbe number of about 23.256; and an eleventh lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .517, and Abbe number of about 66.2, wherein the optical lens assembly (104) comprises an aperture stop positioned between the fifth lens element and the sixth lens element, wherein the aperture stop is a planar element having a thickness of about 0.05 mm.

23. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1 X, a numerical aperture of about 0.042, a length of about 10.685 mm, a half field of view of about 23 degree, a depth of field of about 336.32 micron, and an airy radius of about 8.575 micron, wherein the optical lens assembly (104) comprises twelve lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.849 mm, an image side surface of a radius of curvature of about -1 .05 mm, a thickness of about 0.46 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a third lens element having an object side surface of a radius of curvature of about -1 .04 mm, an image side surface of a radius of curvature of about -0.686 mm, a thickness of about 0.373 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a fourth lens element having an object side surface of a radius of curvature of about 0.763 mm, an image side surface of a radius of curvatureof about 0.64 mm, a thickness of about 0.583 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a fifth lens element having an object side surface of a radius of curvature of about 0.0448 mm, an image side surface of a radius of curvature of about 0.272 mm, a thickness of about 0.3 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about 0.0328 mm, an image side surface of a radius of curvature of about -0.481 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a seventh lens element having an object side surface of a radius of curvature of about 0.535 mm, an image side surface of a radius of curvature of about -0.0777 mm, a thickness of about 0.99 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eighth lens element having an object side surface of a radius of curvature of about -0.0129 mm, an image side surface of a radius of curvature of about 0.319 mm, a thickness of about 0.3 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.128 mm, an image side surface of a radius of curvature of about -0.186 mm, a thickness of about 0.49 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a tenth lens element having an object side surface of a radius of curvature of about -0.268 mm, an image side surface of a radius of curvature of about -0.579 mm, a thickness of about 0.89 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eleventh lens element having an object side surface of a radius of curvature of about -0.267 mm, an image side surface of a radius of curvatureof about 0.298 mm, a thickness of about 0.5 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 , wherein the optical lens assembly (104) comprises an aperture stop positioned between the sixth lens element and the seventh lens element, wherein the aperture stop is a planar element having a thickness of about 0.186 mm.

24. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1 X, a numerical aperture of about 0.103, a length of about 8.331 mm, a half field of view of about 21 degree, a depth of field of about 55.51 micron, and an airy radius of about 3.487 micron, wherein the optical lens assembly (104) comprises twelve lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.075 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -0.596 mm, an image side surface of a radius of curvature of about 0.535 mm, a thickness of about 0.25 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a third lens element having an object side surface of a radius of curvature of about 1.16 mm, an image side surface of a radius of curvature of about 0.536 mm, a thickness of about 0.445 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a fourth lens element having an object side surface of a radius of curvature of about 0.373 mm, an image side surface of a radius of curvatureof about 0.256 mm, a thickness of about 0.245 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a fifth lens element having an object side surface of a radius of curvature of about -0.639 mm, an image side surface of a radius of curvature of about 0.0257 mm, a thickness of about 0.15 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about 0.155 mm, an image side surface of a radius of curvature of about -1.07 mm, a thickness of about 0.495 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; a seventh lens element having an object side surface of a radius of curvature of about 0.481 mm, an image side surface of a radius of curvature of about -0.0328 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; an eighth lens element having an object side surface of a radius of curvature of about -0.272 mm, an image side surface of a radius of curvature of about -0.0448 mm, a thickness of about 0.30 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.64 mm, an image side surface of a radius of curvature of about -0.763 mm, a thickness of about 0.583 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; a tenth lens element having an object side surface of a radius of curvature of about 0.686 mm, an image side surface of a radius of curvature of about 1 .04 mm, a thickness of about 0.373 mm, a refractive index of about 1 .53, and Abbe number of about 55.8; an eleventh lens element having an object side surface of a radius of curvature of about 1 .05 mm, an image side surface of a radius of curvatureof about 0.849 mm, a thickness of about 0.46 mm, a refractive index of about1 .53, and Abbe number of about 55.8; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .52, and Abbe number of about 64.2, wherein the optical lens assembly (104) comprises an aperture stop positioned between the sixth lens element and the seventh lens element, wherein the aperture stop is a planar element having a thickness of about 0.50 mm.

25. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1 .5X, a numerical aperture of about 0.073, a length of about 12.841 mm, a half field of view of about 13 degrees, a depth of field of about 47.95 micron, and an airy radius of about 4.964 micron, wherein the optical lens assembly (104) comprises twelve lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.3 mm, a refractive index of about 1 .515, and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -0.676 mm, an image side surface of a radius of curvature of about -0.6 mm, a thickness of about 0.842 mm, a refractive index of about1 .54, and Abbe number of about 56.1 ; a third lens element having an object side surface of a radius of curvature of about 0.693 mm, an image side surface of a radius of curvature of about 1.1 mm, a thickness of about 0.494 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a fourth lens element having an object side surface of a radius of curvature of about 0.512 mm, an image side surface of a radius of curvatureof about 0.126 mm, a thickness of about 0.58 mm, a refractive index of about1 .54, and Abbe number of about 56.1 ; a fifth lens element having an object side surface of a radius of curvature of about -0.368 mm, an image side surface of a radius of curvature of about -0.13 mm, a thickness of about 0.23 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a sixth lens element having an object side surface of a radius of curvature of about -0.006 mm, an image side surface of a radius of curvature of about -0.593 mm, a thickness of about 0.643 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a seventh lens element having an object side surface of a radius of curvature of about 0.421 mm, an image side surface of a radius of curvature of about 0.02 mm, a thickness of about 0.644 mm, a refractive index of about1 .54, and Abbe number of about 55.9; an eighth lens element having an object side surface of a radius of curvature of about 0.136 mm, an image side surface of a radius of curvature of about 0.371 mm, a thickness of about 0.432 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.0745 mm, an image side surface of a radius of curvature of about -0.217 mm, a thickness of about 1.34 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a tenth lens element having an object side surface of a radius of curvature of about 0.16 mm, an image side surface of a radius of curvature of about 0.01 mm, a thickness of about 0.55 mm, a refractive index of about1 .54, and Abbe number of about 55.9; an eleventh lens element having an object side surface of a radius of curvature of about 0.31 mm, an image side surface of a radius of curvatureof about 0.667 mm, a thickness of about 0.55 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.3 mm, a refractive index of about 1 .52, and Abbe number of about 64.2, wherein the optical lens assembly (104) comprises an aperture stop positioned between the sixth lens element and the seventh lens element, wherein the aperture stop is a planar element having a thickness of about 0.5 mm.

26. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 2.6X, a numerical aperture of about 0.0046, a length of about 8.89 mm, a half field of view of about 20 degree, a depth of field of about 75.352 micron, and an airy radius of about 8.89 micron, wherein the optical lens assembly (104) comprises twelve lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1.151 , and Abbe number of about 64.1 ; a second lens element having an object side surface of a radius of curvature of about -1 .35 mm, an image side surface of a radius of curvature of about -1 .2 mm, a thickness of about 0.412 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a third lens element having an object side surface of a radius of curvature of about 1 .39 mm, an image side surface of a radius of curvature of about 2.2 mm, a thickness of about 0.247 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a fourth lens element having an object side surface of a radius of curvature of about 1 .02 mm, an image side surface of a radius of curvatureof about 0.252 mm, a thickness of about 0.29 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a fifth lens element having an object side surface of a radius of curvature of about -0.737 mm, an image side surface of a radius of curvature of about -0.26 mm, a thickness of about 0.1 15 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a sixth lens element having an object side surface of a radius of curvature of about -0.0121 mm, an image side surface of a radius of curvature of about -1.19 mm, a thickness of about 0.322 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a seventh lens element having an object side surface of a radius of curvature of about 0.535 mm, an image side surface of a radius of curvature of about -0.0777 mm, a thickness of about 0.99 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eighth lens element having an object side surface of a radius of curvature of about -0.0129 mm, an image side surface of a radius of curvature of about 0.319 mm, a thickness of about 0.3 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a ninth lens element having an object side surface of a radius of curvature of about -0.128 mm, an image side surface of a radius of curvature of about -0.186 mm, a thickness of about 0.49 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a tenth lens element having an object side surface of a radius of curvature of about -0.268 mm, an image side surface of a radius of curvature of about -0.579 mm, a thickness of about 0.89 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; an eleventh lens element having an object side surface of a radius of curvature of about -0.267 mm, an image side surface of a radius of curvatureof about 0.298 mm, a thickness of about 0.5 mm, a refractive index of about 1 .54, and Abbe number of about 56.2; and a twelfth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.15 mm, a refractive index of about 1 .52, and Abbe number of about 64.1 , wherein the optical lens assembly (104) comprises an aperture stop positioned between the sixth lens element and the seventh lens element, wherein the aperture stop is a planar element having a thickness of about 0.5 mm.

27. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 2X, a numerical aperture of about 0.044, a length of about 7.424 mm, a half field of view of about 20 degree, a depth of field of about 76.56 micron, and an airy radius of about 8.401 micron, wherein the optical lens assembly (104) comprises twelve lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.075 mm, a refractive index of about 1 .52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -2.67 mm, an image side surface of a radius of curvature of about -1.24 mm, a thickness of about 0.138 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a third lens element having an object side surface of a radius of curvature of about -0.04 mm, an image side surface of a radius of curvature of about -0.639 mm, a thickness of about 0.138 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a fourth lens element having an object side surface of a radius of curvature of about 0.868 mm, an image side surface of a radius of curvatureof about 0.298 mm, a thickness of about 0.335 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a fifth lens element having an object side surface of a radius of curvature of about -1 .49 mm, an image side surface of a radius of curvature of about -0.543 mm, a thickness of about 0.108 mm, a refractive index of about 1 .63, and Abbe number of about 23.4; a sixth lens element having an object side surface of a radius of curvature of about -0.08 mm, an image side surface of a radius of curvature of about -1.68 mm, a thickness of about 0.161 mm, a refractive index of about 1 .54, and Abbe number of about 55.9; a seventh lens element having an object side surface of a radius of curvature of about 0.593 mm, an image side surface of a radius of curvature of about 0.006 mm, a thickness of about 0.403 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; an eighth lens element having an object side surface of a radius of curvature of about 0.13 mm, an image side surface of a radius of curvature of about 0.368 mm, a thickness of about 0.23 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a ninth lens element having an object side surface of a radius of curvature of about -0.126 mm, an image side surface of a radius of curvature of about -0.512 mm, a thickness of about 0.58 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; a tenth lens element having an object side surface of a radius of curvature of about -1 .1 mm, an image side surface of a radius of curvature of about -0.693 mm, a thickness of about 0.494 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; an eleventh lens element having an object side surface of a radius of curvature of about 0.6 mm, an image side surface of a radius of curvatureof about 0.676 mm, a thickness of about 0.842 mm, a refractive index of about 1 .54, and Abbe number of about 56.1 ; and a twelfth lens element having a planar object side surface, a planar image side surface, and a thickness of about 0.3 mm, a refractive index of about 1 .515, and Abbe number of about 63.96, wherein the optical lens assembly (104) comprises an aperture stop positioned between the sixth lens element and the seventh lens element, wherein the aperture stop is a planar element having a thickness of about 0.259 mm.

28. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1X, a numerical aperture of about 0.189, a length of about 12.212 mm, a half field of view of about 70 degree, a depth of field of about 17.5 micron, and an airy radius of about 1.97 micron, wherein the optical lens assembly (104) comprises thirteen lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 1 .2 mm, a refractive index of about 64.167, and Abbe number of about 1 .517; a second lens element having a planar object side surface, a planar image side surface, a thickness of about 0.5 mm, a refractive index of about 1 .517, and Abbe number of about 64.167; a third lens element having an object side surface of a radius of curvature of about -2.17 mm, an image side surface of a radius of curvature of about 14.7 mm, a thickness of about 0.2 mm, a refractive index of about 1 .531 , and Abbe number of about 56.043; a fourth lens element having an object side surface of a radius of curvature of about -8.9 mm, an image side surface of a radius of curvature of about -9.15 mm, a thickness of about 0.3 mm, a refractive index of about 1 .585, and Abbe number of about 29.909;a fifth lens element having an object side surface of a radius of curvature of about 3.5 mm, an image side surface of a radius of curvature of about -3.1 mm, a thickness of about 1 .2 mm, a refractive index of about1 .531 , and Abbe number of about 56.04; a sixth lens element having a planar object side surface, an image side surface of a radius of curvature of about 4.2 mm, a thickness of about 0.2 mm, a refractive index of about 1 .531 , and Abbe number of about 23.9; a seventh lens element having an object side surface of a radius of curvature of about -16.6 mm, an image side surface of a radius of curvature of about -2.83 mm, a thickness of about 0.5 mm, a refractive index of about1 .531 , and Abbe number of about 56.044; an eighth lens element having an object side surface of a radius of curvature of about 3.25 mm, an image side surface of a radius of curvature of about 20 mm, a thickness of about 0.5 mm, a refractive index of aboutl .531 , and Abbe number of about 56.043; a ninth lens element having an object side surface of a radius of curvature of about -3.54 mm, a planar image side surface, a thickness of about 0.2 mm, a refractive index of about 1 .634, and Abbe number of about 23.9; a tenth lens element having an object side surface of a radius of curvature of about 3.1 mm, an image side surface of a radius of curvature of about -3.5 mm, a thickness of about 1 .2 mm, a refractive index of about1 .531 , and Abbe number of about 56.04; an eleventh lens element having an object side surface of a radius of curvature of about 13.5 mm, an image side surface of a radius of curvature of about -58.8 mm, a thickness of about 0.3 mm, a refractive index of about 1 .585, and Abbe number of about 29.91 ; a twelfth lens element having an object side surface of a radius of curvature of about -6 mm, an image side surface of a radius of curvature ofabout 2.4 mm, a thickness of about 0.2 mm, a refractive index of about 1 .531 , and Abbe number of about 56.04; and a thirteenth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.25 mm, a refractive index of about 1 .5168, and Abbe number of about 64.16, wherein the optical lens assembly (104) comprises an aperture stop positioned between the seventh lens element and the eighth lens element, wherein the aperture stop is a planar element having a thickness of about 0.1 mm.

29. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1 X, a numerical aperture of about 0.142, a length of about 7.816 mm, a half field of view of about 10 degree, a depth of field of about 2.47 micron, and an airy radius of about 31.83 micron, wherein the optical lens assembly (104) comprises fourteen lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 0.105 mm, a refractive index of about 1 .52, and Abbe number of about 64.2; a second lens element having an object side surface of a radius of curvature of about -0.331 mm, an image side surface of a radius of curvature of about 0.424 mm, a thickness of about 0.19 mm, a refractive index of about 1 .51 , and Abbe number of about 56.5; a third lens element having an object side surface of a radius of curvature of about -0.606 mm, an image side surface of a radius of curvature of about -1.28 mm, a thickness of about 0.249 mm, a refractive index of about 1 .54, and Abbe number of about 56.0; a fourth lens element having an object side surface of a radius of curvature of about 1 .08 mm, an image side surface of a radius of curvatureof about 1 .64 mm, a thickness of about 0.185 mm, a refractive index of about 1 .61 , and Abbe number of about 26.9; a fifth lens element having an object side surface of a radius of curvature of about 0.404 mm, an image side surface of a radius of curvature of about -0.124 mm, a thickness of about 0.303 mm, a refractive index of about 1 .54, and Abbe number of about 56.0; a sixth lens element having an object side surface of a radius of curvature of about -0.233 mm, an image side surface of a radius of curvature of about -0.107 mm, a thickness of about 0.1 16 mm, a refractive index of about 1 .67, and Abbe number of about 19.5; a seventh lens element having an object side surface of a radius of curvature of about -0.91 1 mm, an image side surface of a radius of curvature of about -0.521 mm, a thickness of about 0.1 15 mm, a refractive index of about 1 .61 , and Abbe number of about 26.9; an eighth lens element having an object side surface of a radius of curvature of about -0.0225 mm, an image side surface of a radius of curvature of about -0.868 mm, a thickness of about 0.392 mm, a refractive index of about 1 .54, and Abbe number of about 56.0; a ninth lens element having an object side surface of a radius of curvature of about 0.313 mm, an image side surface of a radius of curvature of about 0.127 mm, a thickness of about 0.38 mm, a refractive index of about 1 .53, and Abbe number of about 55.7; a tenth lens element having an object side surface of a radius of curvature of about -0.0999 mm, an image side surface of a radius of curvature of about -0.913 mm, a thickness of about 0.471 mm, a refractive index of about 1 .53, and Abbe number of about 55.7; an eleventh lens element having an object side surface of a radius of curvature of about -2.12 mm, an image side surface of a radius of curvatureof about -1.14 mm, a thickness of about 0.22 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; a twelfth lens element having an object side surface of a radius of curvature of about 0.218 mm, an image side surface of a radius of curvature of about -1.14 mm, a thickness of about 0.694 mm, a refractive index of about 1 .53, and Abbe number of about 55.7; a thirteenth lens element having an object side surface of a radius of curvature of about 0.354 mm, an image side surface of a radius of curvature of about 1 .29 mm, a thickness of about 0.482 mm, a refractive index of about 1 .64, and Abbe number of about 23.3; and a fourteenth lens element having a planar object side surface, a planar image side surface, a thickness of about 0.21 mm, a refractive index of about 1 .515, and Abbe number of about 64.1 , wherein the optical lens assembly (104) comprises an aperture stop positioned between the eighth lens element and the ninth lens element, wherein the aperture stop is a planar element having a thickness of about 0.5 mm.

30. The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 4.5X, a numerical aperture of about 0.438, a length of about 23.022 mm, a half field of view of about 70 degree, a depth of field of about 2.847 micron, and an airy radius of about 0.783 micron, wherein the optical lens assembly (104) comprises eleven lens elements having: a first lens element having a planar object side surface, a planar image side surface, a thickness of about 1 mm, a refractive index of about 1 .517, and Abbe number of about 63.96; a second lens element having a planar object side surface, a planar image side surface, a thickness of about 0.25 mm, a refractive index of about 1 .517, and Abbe number of about 63.96;a third lens element having an object side surface of a radius of curvature of about -2 mm, an image side surface of a radius of curvature of about 6.6 mm, a thickness of about 0.3 mm, a refractive index of about 1 .64, and Abbe number of about 23.9; a fourth lens element having an object side surface of a radius of curvature of about 3.39 mm, an image side surface of a radius of curvature of about -1 .8 mm, a thickness of about 1 .8 mm, a refractive index of about1 .517, and Abbe number of about 63.96; a fifth lens element having an object side surface of a radius of curvature of about 2.45 mm, an image side surface of a radius of curvature of about 5.67 mm, a thickness of about 1 .3 mm, a refractive index of about1 .517, and Abbe number of about 63.96; a sixth lens element having an object side surface of a radius of curvature of about -2.5 mm, an image side surface of a radius of curvature of about 2.6 mm, a thickness of about 0.3 mm, a refractive index of about 1 .643, and Abbe number of about 23.9; a seventh lens element having an object side surface of a radius of curvature of about 1 .27 mm, an image side surface of a radius of curvature of about 7.6 mm, a thickness of about 0.75 mm, a refractive index of about 1 .586, and Abbe number of about 30; an eighth lens element having an object side surface of a radius of curvature of about 2.82 mm, an image side surface of a radius of curvature of about 4.3 mm, a thickness of about 0.5 mm, a refractive index of about1 .517, and Abbe number of about 63.96; a ninth lens element having an object side surface of a radius of curvature of about -1 .38 mm, an image side surface of a radius of curvature of about -2.16 mm, a thickness of about 0.3 mm, a refractive index of about 1 .586, and Abbe number of about 30; a tenth lens element having an object side surface of a radius of curvature of about 2 mm, an image side surface of a radius of curvature ofabout 3.3 mm, a thickness of about 0.75 mm, a refractive index of about 1 .535, and Abbe number of about 56; and an eleventh lens element having a planar object side surface, a planar image side surface, a thickness of about 0.5 mm, a refractive index of about 1 .517, and Abbe number of about63.96, wherein the optical lens assembly (104) comprises an aperture stop positioned between the seventh lens element and the eighth lens element, wherein the aperture stop is a planar element having a thickness of about 0.2 mm.31 . The microscopy imaging system (222) as claimed in claim 1 , wherein the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.6 micron; orthe optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 1 X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.5 micron; orthe optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 1 ,4X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 1.5X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 1.5X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 0.4 micron; orthe optical lens assembly (104) has an optical magnification of about 1 .5X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 1 .6X and the image sensor (106) has a pixel pitch of about 0.2 micron; orthe optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 1 .8X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 6 micron; orthe optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.56 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 2X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 6 micron; orthe optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 2.4X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 3 micron; orthe optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 0.56 micron; or the optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 2.6X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.6 micron; orthe optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 3X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.5 micron; orthe optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 4X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 0.56 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 4.5X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 1 micron; orthe optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 5X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.9 micron; orthe optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.8 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 6X and the image sensor (106) has a pixel pitch of about 0.2 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 6 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 3 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 1 .4 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 1.12 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 1 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.9 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.8 micron; orthe optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.7 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.6 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.5 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.4 micron; or the optical lens assembly (104) has an optical magnification of about 7.8X and the image sensor (106) has a pixel pitch of about 0.2 micron.

32. The microscopy imaging system (222) as claimed in claim 1 , wherein the compact optical microscopy device (102) comprises a sample holding unit, wherein the sample holding unit includes at least one compartment to hold a sample to be imaged by the optical lens assembly (104).

33. The microscopy imaging system (222) as claimed in claim 32, wherein the sample holding unit is magnetically coupled to a housing of the microscopy imaging system (222) for imaging the sample by the optical lens assembly (104).

34. The microscopy imaging system (222) as claimed in claim 33, wherein the sample holding unit is movable at least in one direction in a plane in which the housing extends for imaging the sample by the optical lens assembly (104).

35. The microscopy imaging system (222) as claimed in claim 32, wherein the sample holding unit has at least one planar surface having a wavelength-specific filter.

36. The microscopy imaging system (222) as claimed in claim 32, wherein the sample holding unit comprises a top cover to cover the at least one compartment, wherein the top cover is a wavelength-specific filter.

37. The microscopy imaging system (222) as claimed in claim 32, wherein the sample holding unit comprises a sample injection port corresponding to each of the at least one compartment.

38. The microscopy imaging system (222) as claimed in claim 32, wherein the compact optical microscopy device (102) comprises a first wavelengthspecific filter disposed on a first planar side of the at least one compartment.

39. The microscopy imaging system (222) as claimed in claim 38, wherein the compact optical microscopy device (102) comprises a second wavelength-specific filter disposed on a second planar side of the at least one compartment, wherein the second planar side is opposite to the first planar side.

40. The microscopy imaging system (222) as claimed in claim 32, wherein the at least one compartment comprises an inlet for inflow of the sample and an outlet for outflow of the sample.

41. The microscopy imaging system (222) as claimed in claim 10, wherein the compact optical microscopy device (102) comprises a second light source to provide light from a second side to the sample in the sample holding unit, wherein the second side is opposite to the first side.

42. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source is movable relative to the optical lens assembly (104) to vary a distance between a planar surface of the sample holding unit.

43. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source is a point light source or a diffuse light source or a combination thereof, and is detachably mounted in a housing of the second light source.

44. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source has at least one of variable intensity of light, variablewavelength of light, and variable area of light exposure on the sample in the sample holding unit.

45. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source comprises a housing and a cavity with electrical contacts to mount a white LED or a wavelength-specific LED.

46. The microscopy imaging system (222) as claimed in claim 41 , wherein the compact optical microscopy device (102) comprises a diffuser or a polarized filter between the second light source and the sample holding unit.

47. The microscopy imaging system (222) as claimed in claim 41 , wherein compact optical microscopy device (102) comprised a convex lens between the second light source and the sample holding unit.

48. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source is a white LED.

49. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source comprises a white LED, and wherein the compact optical microscopy device (102) comprises a convex lens and a ring shaped aperture, wherein the convex lens is placed to direct the light, from the white LED, passing through the ring shaped aperture onto the sample in the sample holder unit.

50. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source comprises a wavelength-specific LED emitting light of a wavelength in a range of about 200 nm to about 700 nm, and wherein the microscopy imaging system (222) comprises a convex lens and a ring shaped aperture, wherein the convex lens is placed to direct the light, from the white LED, passing through the ring shaped aperture onto the sample in the sample holder unit.

51. The microscopy imaging system (222) as claimed in claim 41 , wherein the second light source comprises: a housing with a reflective surface; andtwo LEDs mounted on opposite surfaces to reflect light onto the sample in the sample holding unit.

52. The microscopy imaging system (222) as claimed in claim 1 , wherein the processing unit (228) is one of a standalone processing unit and a cloud processing unit.

53. A method for processing an image of an object, the method comprising: capturing an image of an object using an image sensor (106) through an optical lens assembly (104) having eight to sixteen lens elements, wherein the image sensor (106) has a pixel pitch in a range of about 0.2 micron to about 6 micron, wherein the optical lens assembly (104) has: an optical magnification in a range of about 1X to about 7.8X; an airy radius in a range of about 0.783 micron to about 23.25 micron; a depth of field in a range of about 2.847 micron to about 338 micron; a numerical aperture in a range of about 0.015 to about 0.438; a half field of view in a range of about 13 degrees to about 70 degrees; and a length in a range of about 5.51 1 millimeter (mm) to about 57 mm; and processing, by a processing unit (228), the captured image by converting the image to a digitized form, wherein the processing unit (228) is communicatively coupled to a compact optical microscopy device (102) having the image sensor (106) and the optical lens assembly (104), wherein the processing unit (228) is disposed within the compact opticalmicroscopy device (102) or outside the compact optical microscopy device (102); detecting, by the processing unit (228), a unique or multiple characteristic feature of the object by analyzing the digitized form of the image using an artificial intelligence and machine learning based model; comparing, by the processing unit (228), the detected characteristic feature with pre-stored dataset, wherein for the comparison, the detected characteristic feature is mapped with a relevant parameter, of the object , present in the pre-stored dataset; and generating, by the processing unit (228), a report based on the comparison, wherein the report is to identify a category of the detected characteristic feature of the object with or without difference or similarity in the detected characteristic feature.

54. The method as claimed in claim 53, wherein the method comprises transferring, by the processing unit (228), data related to the category of the unique or multiple characteristic feature of the image to a cloud database.

55. The method as claimed in claim 53, wherein the method comprises transferring, by the processing unit (228), data related to the category of the unique or multiple characteristic feature of the image to one of an inter device, an intra device, and a cloud device using a wired or a wireless communication channel.

56. The method as claimed in claim 53, wherein the method comprises displaying, on a display unit, connected to the processing unit (228), the identified category of the unique or multiple characteristic feature in the form of one of an image, a text, and a report.

57. The method as claimed in claim 53, wherein the method comprises transmitting, via a transmitter coupled to the processing unit (228), the generated report via the transmitter to another device.

Images