Wide-field illumination system, biological sample recognition method, and sequencing device

Abstract

Provided in the present application is a wide-field illumination system, comprising: a light source assembly, which is used for emitting first light and second light, which have different wavelengths; an angle adjustment assembly, which comprises a prism located on light paths of the first light and the second light, wherein the prism emits the received first light and second light at different angles; and a light modulation assembly, which comprises a structured light generation element located on the light paths of the first light and the second light, wherein the structured light generation element can modulate the light intensities and phases of the received first light and second light so as to generate structured light, the structured light comprises first diffracted light formed on the basis of the first light and second diffracted light formed on the basis of the second light, and the first light and the second light which are from the prism are incident to the structured light generation element at different incident angles, such that a target diffraction order of the first diffracted light coincides with that of the second diffracted light. Further provided in the present application are a biological sample recognition method and a sequencing device.

Description

Wide-field illumination system, biological sample identification method, and sequencing equipment Technical Field

[0001] The present application relates to the field of microscopic imaging technology, and in particular to a wide-field illumination system, a biological sample identification method, and a sequencing device. Background Art

[0002] Gene sequencing can be performed using structured illumination microscopy (SIM) technology.

[0003] An optical system utilizing SIM technology includes a light source and a dimming device. The light source emits light, and the dimming device (such as a physical grating or a digital grating) modulates the light from the light source to produce structured illumination. The dimming device also produces a diffraction effect during the modulation of the light from the light source, resulting in the structured illumination produced by the dimming device being diffracted light.

[0004] Based on the principle of diffraction, the diffraction angle and diffraction order of a light beam are closely related to its wavelength. For light sources of different wavelengths, after being modulated by a dimming device, the resulting structured illumination light has different diffraction angles, resulting in dispersion. Due to the existence of dispersion, when the light source includes multiple wavelengths, the diffraction orders corresponding to the different wavelengths in the generated structured illumination light cannot overlap. As a result, the target diffraction orders corresponding to the different wavelengths of light are difficult to be utilized by the system simultaneously, resulting in low light energy utilization, low contrast of the structured illumination light, and poor imaging quality.

[0005] Summary of the Invention

[0006] A first aspect of the present application provides a wide-field lighting system, comprising:

[0007] A light source assembly for emitting a first light and a second light having different wavelengths;

[0008] an angle adjustment component, comprising a prism located on the optical paths of the first light and the second light, the prism emitting the received first light and the second light at different angles;

[0009] a light modulation component, comprising a structured light generating element located on an optical path of the first light and the second light, wherein the structured light generating element can modulate the intensity and phase of the received first light and the second light to generate structured light, wherein the structured light is used to illuminate a sample to be measured to form an image;

[0010] In which, the structured light includes a first diffraction light formed based on the first light and a second diffraction light formed based on the second light, and the first light and the second light from the prism are incident on the structured light generating element at different incident angles so that the target diffraction order of the first diffraction light and the second diffraction light coincides.

[0011] A second aspect of the present application provides a biological sample identification method, comprising:

[0012] The light source assembly emits a first light and a second light having different wavelengths;

[0013] The light paths of the first light and the second light are emitted through a prism at different angles;

[0014] The first light and the second light emitted from the prism are received by a structured light generating element. The structured light generating element modulates the intensity and phase of the received first light and second light to generate structured light. The structured light includes a first diffracted light formed based on the first light and a second diffracted light formed based on the second light. The first light and the second light from the prism are incident on the structured light generating element at different incident angles so that target diffraction orders of the first diffracted light and the second diffracted light coincide with each other.

[0015] The structured light irradiates the sample to be tested to generate detection light, and the detection light is received to generate an image; and

[0016] Information of the biological sample is detected based on the image recognition.

[0017] A third aspect of the present application provides a sequencing device, comprising:

[0018] A light source assembly for emitting a first light and a second light having different wavelengths;

[0019] an angle adjustment component, comprising a prism located on the optical paths of the first light and the second light, the prism emitting the received first light and the second light at different angles;

[0020] a light modulation component comprising a structured light generating element located in an optical path of the first light and the second light, the structured light generating element being capable of modulating the intensity and phase of the received first light and the second light to generate structured light, the structured light comprising a first diffracted light formed based on the first light and a second diffracted light formed based on the second light, the first light and the second light from the prism being incident on the structured light generating element at different incident angles so that target diffraction orders of the first diffracted light and the second diffracted light coincide with each other;

[0021] a sample carrier, the sample carrier being used to support a sample to be tested, the sample to be tested receiving the first diffracted light and the second diffracted light and generating detection light,

[0022] an imaging assembly including a camera that receives the detection light and generates an image;

[0023] A controller is electrically connected to the camera and is used to perform super-resolution reconstruction based on the image to generate a super-resolution image, and to obtain base sequence information of the sample to be tested based on the super-resolution image.

[0024] In addition, the present application also provides a biometric identification device, comprising:

[0025] A light source assembly for emitting a first light and a second light having different wavelengths;

[0026] an angle adjustment component, comprising a prism located on the optical paths of the first light and the second light, the prism emitting the received first light and the second light at different angles;

[0027] a light modulation component comprising a structured light generating element located in an optical path of the first light and the second light, the structured light generating element being capable of modulating the intensity and phase of the received first light and the second light to generate structured light, the structured light comprising a first diffracted light formed based on the first light and a second diffracted light formed based on the second light, the first light and the second light from the prism being incident on the structured light generating element at different incident angles so that target diffraction orders of the first diffracted light and the second diffracted light coincide with each other;

[0028] An imaging component, comprising a camera located at the structured light, the camera collecting an image based on detection light generated after the structured light illuminates the sample to be measured;

[0029] A controller is electrically connected to the camera and is used to perform super-resolution reconstruction based on the image to generate a super-resolution image, and to obtain biological information of the sample to be tested based on the super-resolution image.

[0030] The wide-field illumination system, biological sample identification method, and sequencing device described above utilize the dispersion characteristics of optical glass to split the first light and the second light of different wavelengths through a prism, and emit the received first light and the second light at different angles, so that the incident angles of the first light and the second light when incident on the structured light generating element are different. The difference in the incident angles of the two makes it possible for the target diffraction orders of the first diffracted light and the second diffracted light formed after diffraction by the structured light generating element to coincide with each other; that is, according to the diffraction principle, the first diffracted light generated by the first light and the second light of different wavelengths coincides with each other. The target diffraction order of the second diffraction light is difficult to overlap, and the diffraction angles of the same diffraction order of the first diffraction light and the second diffraction light are different. The present application uses a prism to make the first light and the second light incident on the structured light generating element at different incident angles, which can compensate for the difference between the diffraction angles of the same diffraction order in the first diffraction light and the second diffraction light, so that the target diffraction order of the first diffraction light and the second diffraction light can overlap; therefore, the target diffraction orders of the first diffraction light and the second diffraction light can be used by the system at the same time, which is beneficial to improving the utilization rate of light energy, improving the contrast of structured light and improving the signal-to-noise ratio of the system. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] FIG1 is a schematic diagram of the module structure of a biometric recognition device according to an embodiment of the present application.

[0032] FIG. 2 is a schematic diagram of the optical path structure of the biometric recognition device in FIG. 1 .

[0033] FIG3 is a schematic diagram of the planar structure of the structured light generating element in FIG2 .

[0034] FIG4 is a schematic diagram of the structure of stripe patterns formed by different structured lights.

[0035] FIG. 5 is a schematic diagram of the diffraction effect of the structured light generating element in FIG. 2 on light in the initial state.

[0036] FIG. 6 is a schematic diagram showing the diffraction effect of the structured light generating element in FIG. 2 on light after being powered on.

[0037] FIG. 7 is a schematic diagram of the diffraction effect of the structured light generating element in FIG. 2 on light when a stripe pattern is loaded.

[0038] FIG. 8 is a schematic diagram showing the diffraction effect of a structured light generating element on light.

[0039] FIG. 9 is another schematic diagram of the diffraction effect of the structured light generating element on light.

[0040] FIG10 shows the corresponding relationship between the diffraction angle and the incident angle of the target diffraction order in the first diffracted light and the second diffracted light.

[0041] FIG. 11 is a schematic diagram illustrating the refraction process of the first light and the second light by the prism in FIG. 2 .

[0042] FIG12 is a schematic diagram showing the propagation paths of the first light and the second light in the biometric recognition device.

[0043] Figure 13 shows the overlap of light spots on the spectrum plane.

[0044] FIG14 shows the overlap of the light spot on the modulation surface of the structured light generating element and its conjugate surface.

[0045] FIG15 is a schematic diagram of a biological sample identification method provided in an embodiment of the present application.

[0046] Description of the main component symbols Biometric identification device 100 Sample to be tested 200 Platform 300 Light source assembly 10 First light-emitting element 11 Second light-emitting element 12 Multimode optical fiber 13 First lens 14 Second lens 15 Angle adjustment assembly 20 Prism 21 Light-receiving surface 211 Light-emitting surface 212 Third lens 22 Fourth lens 23 Reflectors 16, 24, 25, 26 Light modulation assembly 30 Structured light generating element 31 Micro-reflector 311 Modulation surface 312 Beam reduction lens 32 Imaging assembly 40 First imaging channel 41Second imaging channel 42 Filters 411, 421 Tube lenses 412, 422 Cameras 413, 423 Objective lens 43 Spectral element 44 Controller 50 Light guiding element 60 First light L11 Second light L12 Structured light L2 Detection light L3 First detection light L31 Second detection light L32 Light spots S1, S2 Steps S11, S12, S13, S14, S15

[0047] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0048] In order to more clearly understand the above-mentioned objectives, features and advantages of the present application, the present application is described in detail below in conjunction with the accompanying drawings and specific embodiments. It should be noted that the embodiments of the present application and the features therein can be combined with each other in the absence of conflict.

[0049] The following description sets forth many specific details to facilitate a full understanding of the present application. The embodiments described are only a portion of the embodiments of the present application, not all of the embodiments. All other embodiments derived by persons of ordinary skill in the art based on the embodiments of the present application without creative effort are intended to fall within the scope of protection of the present application.

[0050] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application pertains. The terms used herein in the specification of this application are for the purpose of describing specific embodiments only and are not intended to limit this application.

[0051] The present application provides a wide-field lighting system for use in the field of microscopic imaging technology, for example, it can be applied to structured light super-resolution imaging scenes, projection-based wide-field lighting imaging scenes, beam shaping scenes, and the like. The wide-field lighting system is used to generate structured light with a specific form (such as stripes, multi-focal points, etc.) for illumination. In this embodiment, the application of the wide-field lighting system to the structured light super-resolution imaging scene is used as an example. Specifically, the embodiment of the present application provides a biometric identification device and a biological sample identification method. The following takes the biometric identification device as an example of a sequencing device for detecting nucleic acid sequence information to introduce the optical path structure and working principle of the biometric identification device.

[0052] Referring to Figure 1 , the biometric identification device 100 of the present application is used to detect characteristic information of a sample 200. The sample 200 may be a biological sample, such as a nucleic acid sample (DNA or RNA), protein, or cell. The biometric identification device 100 is used to detect biological information of the sample 200. In this embodiment, the sample 200 is a nucleic acid sample, and the biological information is the base sequence information of the sample 200.

[0053] In this embodiment, the sample 200 to be tested is carried on a platform 300. The platform 300 can translate on the plane where it is located, thereby driving the sample 200 to translate.

[0054] The biometric recognition device 100 includes a light source assembly 10, an angle adjustment assembly 20, a light modulation assembly 30, an imaging assembly 40, and a controller 50. The controller 50 is electrically connected to the light source assembly 10, the light modulation assembly 30, and the imaging assembly 40, respectively, and is used to control the operation of the light source assembly 10, the light modulation assembly 30, and the imaging assembly 40.

[0055] The light source assembly 10 is configured to emit a combined light beam of first light L11 and second light L12, wherein the first light L11 and second light L12 have different wavelengths. The angle adjustment assembly 20 is located in the optical paths of the first light L11 and second light L12 and is configured to change the propagation directions of the first light L11 and second light L12, respectively, to split the first light L11 and second light L12, such that the first light L11 and second light L12 are emitted from the angle adjustment assembly 20 at different angles. The light modulation assembly 30 is located in the optical paths of the first light L11 and second light L12 and is configured to modulate the intensity distribution and phase of the received first light L11 and second light L12 to generate structured light L2 having a specific shape. The light modulation assembly 30 is further configured to reflect the structured light L2 toward the sample 200 to be tested. Different types of bases on the sample 200 to be tested are labeled with different fluorescent substances. When the structured light L2 irradiates the sample 200 to be tested, the different fluorescent substances are excited to produce fluorescence of different wavelengths. In this embodiment, the fluorescence produced by the excited fluorescent substances is defined as detection light L3. The imaging assembly 40 is located in the optical path of the detection light L3 and is configured to receive the detection light L3 and capture multiple images of the sample 200 based on the detection light L3. The controller 50 is configured to control the on / off switching of the light source assembly 10, control the modulation method of the light modulation assembly 30 for modulating the first light L11 and the second light L12, and control the imaging assembly 40 to capture the multiple images. The controller 50 is also configured to perform super-resolution reconstruction on the multiple images to generate a super-resolution image, and to obtain base sequence information of the sample 200 based on the super-resolution image.

[0056] In this embodiment, the biometric recognition device 100 operates through multiple detection cycles, with the platform 300 maintaining a fixed position throughout each detection cycle. After each detection cycle, the platform 300 synchronously translates the sample 200 to the next position, and the biometric recognition device 100 begins the next detection cycle. This translation of the platform 300 completes multiple detection cycles, allowing the structured light L2 to traverse the surface of the sample 200. This completes a complete scan of the sample 200 by the structured light L2.

[0057] Please refer to FIG. 2 , in which the dotted line represents the propagation path of the light (first light L11 , second light L12 , structured light L2 , detection light L3 ), and the arrow on the dotted line represents the propagation direction of the light.

[0058] In this embodiment, the light source assembly 10 includes a first light-emitting element 11, a second light-emitting element 12, a multimode optical fiber 13, a first lens 14, and a second lens 15. The first light-emitting element 11 is configured to emit a first light L11, and the second light-emitting element 12 is configured to emit a second light L12. The first light L11 is red light with a wavelength of 640 nm, and the second light L12 is green light with a wavelength of 532 nm. The multimode optical fiber 13 connects the first light-emitting element 11 and the second light 12, respectively. The first light L11 and the second light L12 are coupled into the multimode optical fiber 13 and are mixed and emitted from the multimode optical fiber 13.

[0059] In this embodiment, both the first light-emitting element 11 and the second light-emitting element 12 are lasers. In other embodiments of the present application, the light source assembly 10 may include a larger number of light-emitting elements. The light-emitting elements in the light source assembly 10 for emitting the first light L11 and the second light L12 may be light-emitting diodes (LEDs) instead of lasers. The first light L11 and the second light L12 may be lights of other colors (or other wavelengths).

[0060] The first lens 14 and the second lens 15 are sequentially positioned along the optical paths of the first light L11 and the second light L12. The first lens 14 is used to expand the first light L11 and the second light L12 to meet the field of view requirements of the subsequent optical path. The second lens 15 is used to receive the first light L11 and the second light L12 emitted by the first lens 14 and convert them into parallel light beams for output. The first light L11 and the second light L12 emitted from the second lens 15 are parallel beams with uniform intensity distribution.

[0061] The angle adjustment component 20 includes a prism 21. The prism 21 has a light receiving surface 211 and a light emitting surface 212. As shown in FIG2 , the prism 21 is preferably a triangular prism. It should be understood that the prism of the present application can also be selected from other prisms, as long as the light receiving surface 211 and the light emitting surface 212 intersect. The mixed light beam of the first light L11 and the second light L12 from the second lens 15 is incident on the light receiving surface 211 in parallel, and after being refracted twice by the light receiving surface 211 and the light emitting surface 212 respectively, it is finally emitted from the light emitting surface 212. The first light L11 and the second light L12 have different wavelengths. According to the law of refraction of light, the refraction angles of the first light L11 and the second light L12 are different each time they are refracted by the prism 21. Therefore, after passing through the prism 21, the mixed light beam of the first light L11 and the second light L12 is split by the prism 21 according to the wavelength, and is emitted from the light emitting surface 212 at different emission angles and incident on the light modulation component 30 along different propagation paths.

[0062] The angle adjustment assembly 20 also includes a 4f lens group located between the prism 21 and the light modulation assembly 30. The 4f lens group includes a third lens 22 and a fourth lens 23 arranged in sequence. The third lens 22 and the fourth lens 23 jointly guide the first light L11 and the second light L12 emitted by the prism 21 to the light modulation assembly 30, so that the light spots formed by the first light L11 and the second light L12 in the light modulation assembly 30 overlap, which helps improve the utilization of light energy.

[0063] In this embodiment, the angle adjustment assembly 20 further includes a reflector 24 between the third lens 22 and the fourth lens 23. The reflector 24 is used to reflect the received first light L11 and the second light L12. The reflector 24 can fold the light path.

[0064] In other embodiments, the angle adjustment assembly 20 may not include the reflector 24 , or may include other optical elements in addition to the reflector 24 to guide light propagation.

[0065] The light modulation assembly 30 includes a structured light generating element 31 and a beam reduction lens 32. The structured light generating element 31 is used to modulate the intensity distribution and phase of the received first light L11 and second light L12 to generate structured light L2. The beam reduction lens 32 is used to scale the structured light L2 to a certain scale and then direct it to the sample 200 to be tested.

[0066] Referring to Figure 3 , in this embodiment, the structured light generating element 31 is a digital micromirror device (DMD). The structured light generating element 31 includes a plurality of aluminum micromirrors 311 arranged in a common plane. The micromirrors 311 are arranged in a micromirror array comprising multiple rows and columns. Each micromirror 311 is generally rectangular. The surface of each micromirror 311 that receives and reflects the first light L11 and the second light L12 constitutes a modulation surface 312 of the structured light generating element 31.

[0067] Each micro-reflector 311 can be deflected around its diagonal. Each micro-reflector 311 has three deflection states: deflected 12° to the left along its diagonal (defined as the "ON" state); deflected -12° to the right along its diagonal (defined as the "OFF" state); and deflected 0° (defined as the initial state). The "ON" state of the micro-reflector 311 is a highly reflective state. When the micro-reflector is in the "ON" state, it reflects light at +12°, so that the structured light L2 is projected onto the surface of the sample to be tested 200 to present a bright field. The "OFF" state of the micro-reflector 311 is a non-reflective state. When the micro-reflector 311 is in the "OFF" state, it reflects light at -12°, so that the structured light L2 appears as a dark field on the surface of the sample to be tested 200. The initial state of the micro-reflector 311 is a non-working state. By adjusting the deflection state of each micro-mirror 311 in the structured light generating element 31 , the shape of the structured light L2 emitted by the structured light generating element 31 can be adjusted (ie, the pattern of the light spot formed by the structured light L2 on the surface of the sample 200 to be tested can be adjusted).

[0068] In this embodiment, the structured light generating element 31 modulates the first light L11 and the second light L12 into striped structured light L2. Specifically, when the structured light L2 is projected onto the sample 200, a light spot pattern consisting of alternating parallel arrangements of light and dark stripes is formed on the surface of the sample 200. By adjusting the deflection states of all micromirrors 311 in the structured light generating element 31, a specific striped pattern can be formed on the surface of the sample 200.

[0069] In other embodiments of the present application, the structured light generating element 31 may be another dimming device, such as a liquid crystal spatial light modulator (LCSLM), a deformable mirror, a microlens array, or a digital grating, or a traditional physical grating. These digital and physical gratings, when modulating the first light L11 and the second light L12, also produce a diffraction effect on the first light L11 and the second light L12.

[0070] A traditional diffraction grating consists of a group of infinitely narrow slits, with the spacing between the slits being d (grating constant). When a plane wave with a wavelength of λ enters the diffraction grating at a specific angle γ, the light can be regarded as a point light source after passing through each slit, emitting a spherical wave and propagating in a fixed direction. The spherical waves emitted by different slits are coherently superimposed. Since the phases of the light emitted from each slit are different at the interference point, they will be superimposed or canceled out. When the optical path difference between the light emitted from two adjacent slits and reaching the interference point is an integer multiple of the wavelength of the light, the two beams of light have the same phase, and the phenomenon of constructive interference light intensity enhancement will occur. Described by the formula, when the diffraction angle θm When the diffraction order m is an integer (0, ±1, ±2, ...), constructive interference occurs at the interference point, that is, the light waves have the same phase, the interference produces the maximum amplitude, and a bright diffraction order is formed. m )=mλ (1)

[0071] According to the grating equation (1), the diffraction angles of different diffraction orders mainly depend on the grating constant d, the diffraction order m and the wavelength λ of the light. For the 0th order diffraction (m = 0), γ and θ m The beams are equal in size but opposite in direction, and only reflect, so the 0th order diffraction of beams of different wavelengths will be emitted at the same diffraction angle. The diffraction angles of other diffraction orders are closely related to the wavelength of the light. When the polychromatic light is incident on the grating, the beam is dispersed so that each wavelength satisfies the grating equation. At the same time, because beams of different wavelengths have different diffraction angles in the non-0th order diffraction order, they will not overlap at the interference point. Usually, for diffracted light, the 0th and ±1st diffraction orders have a high proportion of light energy, which are the target diffraction orders and are expected to be used by the system.

[0072] Similarly, the structured light generating element 31 of this embodiment has a diffraction effect on the first light L11 and the second light L12 when modulating the first light L11 and the second light L12, so that the structured light L2 finally emitted is diffracted light. The structured light L2 includes a first diffracted light and a second diffracted light, the first diffracted light is a diffracted light formed based on the first light L11, and the second diffracted light is a diffracted light formed based on the second light L12. Since the first light L11 and the second light L12 have different wavelengths, according to the above description of the diffraction grating, if the first light L11 and the second light L12 are incident on the structured light generating element 31 in parallel, the diffraction angles of the non-0th order diffraction orders of the first diffracted light and the second diffracted light generated are different, and the light spots do not overlap. This makes it difficult to use the target diffraction orders of the first diffracted light and the second diffracted light at the same time.

[0073] In the biometric authentication device 100, the stripe structured light L2 generated by the structured light generating element 31 is projected onto the sample 200 to be tested. To ensure super-resolution reconstruction at a sufficiently high spatial frequency, the periodic frequency of the structured light L2 needs to achieve a two-fold resolution improvement. Therefore, the biometric authentication device 100 needs to meet a fixed reduction ratio and project the stripe structured light L2 with a certain period onto the sample 200 to be tested. The beam spot size of the structured light L2 is directly related to the size of the modulation surface 312 of the structured light generating element 31 and the focal length of the reduction lens 32.

[0074] Specific parameter (2): The spot size S is positively correlated with the beam quality M, wavelength λ, and the focal length f of the beam reduction lens 32, and is inversely proportional to the beam size l when the structured light L2 enters the beam reduction lens 32.

[0075] Therefore, when the spot size is fixed, due to the dispersion problem caused by the diffraction effect of the structured light generating element 31, the target diffraction orders of the first diffraction light and the second diffraction light cannot coincide, and the target diffraction orders of the first diffraction light and the second diffraction light cannot be used at the same time.

[0076] However, whether the target diffraction orders participate in the generation of structured light L2 has a crucial impact on the quality of structured light L2 illumination. As shown in Figure 4, if the target diffraction orders (0, ±1) participate in the generation of structured light L2, the stripes of structured light L2 have high contrast and signal-to-noise ratio, and the stripes are clear. When only some of the target diffraction orders (for example, only 0 and +1, only 0, only +1, or only -1) participate in the generation of structured light L2, the stripe contrast decreases significantly, or even no stripe pattern can be produced at all.

[0077] Due to the dispersion problem of the structured light generating element 31, the first diffracted light and the second diffracted light cannot simultaneously participate in the generation of the structured light L2, resulting in a decrease in the contrast of the stripes of the structured light L2. At the same time, it also reduces the energy utilization rate of the structured light generating element 31 itself, thereby affecting the signal-to-noise ratio and contrast of the imaging of the biometric recognition device 100, greatly reducing the imaging quality and super-resolution reconstruction effect. In addition, due to the dispersion problem of the first light L11 and the second light L12, the target diffraction order light spots of the first diffracted light and the second diffracted light generated based on the first light L11 and the second light L12 cannot enter the optical element (such as the objective lens) in the subsequent optical path along the main optical axis, and the central imaging field of the objective lens cannot be used. The edge field of view is greatly different, which will also affect the imaging quality of the biometric recognition device 100.

[0078] Therefore, the present application provides an angle adjustment component 20 in the biometric recognition device 100 to solve the above series of problems caused by the diffraction effect of the structured light generating element 31 on light.

[0079] When the structured light generating element 31 of this embodiment is powered on (all micro-mirrors 311 are in the ON state), each micro-mirror 311 is tilted relative to its own diagonal. Since the blazed grating has a sawtooth-like groove surface, the structured light generating element 31 can resemble a blazed grating when powered on. The following explains the diffraction effect of the structured light generating element 31 using the principle of a blazed grating.

[0080] Blazed gratings are mainly plane reflection gratings. Unlike flat grating surfaces, blazed gratings have a series of sawtooth grooves. The angle between the groove surface and the grating surface is called the blaze angle (θ tiltAs a spectroscopic element, a diffraction grating has a large central order of single-slit diffraction that overlaps with the 0th order of interslit interference. The dispersion-free 0th order accounts for a large portion of the total light energy, while the intensity of each of the remaining spectral orders is very weak. In practice, only a certain order of the spectrum may be utilized. A blazed grating, however, can shift the 0th order of single-slit diffraction from the 0th order of interslit interference, shifting and focusing the light energy onto a desired order (which can be the undiffracted 0th order), achieving a blazed modified order spectrum.

[0081] Referring to Figure 5 , before the structured light generating element 31 is powered on (i.e., in the aforementioned initial state, with a deflection of 0°), all micromirrors 311 are flat, meaning the light-reflecting surfaces of the micromirrors 311 are parallel to the grating surface. At this point, the 0th order of the first and second diffracted lights generated by the first light L11 and the second light L12 overlap and occupy the maximum optical energy of all diffraction orders.

[0082] Referring to Figure 6 , after the structured light generating element 31 is powered on, all micro-mirrors 311 are tilted 12 degrees diagonally in the same direction and are all in the ON state. At this point, the structured light generating element 31 can be considered a blazed grating. The first and second diffracted light diffracted by the structured light generating element 31 blazes in the target diffraction orders, occupying the maximum light energy of all diffraction orders, while the energy of the zeroth diffraction order is significantly weakened.

[0083] Referring to Figure 7, when applying stripes with a four-pixel period (the states of four adjacent micromirrors 311 are ON, ON, OFF, OFF, and so on), the positions and energy distributions of the 0th and blazed orders remain unchanged. However, because the stripes applied on top of the DMD's inherent blazed grating also exhibit diffraction effects, the target diffraction orders appear as 0th and ±1st orders. To ensure fringe contrast, all elements must participate in the projection and generation of the fringe structured light onto the object surface.

[0084] In this embodiment, the angle adjustment component 20 adjusts the first light L11 and the second light L12 to have a preset angle difference when incident on the structured light generating element 31. This allows the target diffraction order spots of the diffracted light generated by the first light L11 and the second light L12 to coincide, thereby resolving the aforementioned technical issues. Taking the example of a 640nm wavelength for the first light L11 and a 532nm wavelength for the second light L12 in this embodiment, the target diffraction order and the preset angle difference are determined by the following calculation process:

[0085] For the structured light generating element 31 composed of the DMD of this embodiment, since the single micro-mirror 311 is arranged along the diagonal (45 degrees) axis when flipped, the blaze angle (θ tilt ), angle of incidence (θ i ) and the diffraction angle of the reflected light (θ r) need to be divided by the tangent value Correction by:

[0086] The relationship between the incident angle and diffraction angle and the diffraction order and wavelength is shown in formula (4):

[0087] Formula (5) describes the relationship between the incident angle, reflection angle, and blaze angle: θ i +θ r =2θ tilt (5)

[0088] The blaze condition of the blazed grating is shown in formula (6). If m is an integer, the blaze of the m-level spectrum can be achieved:

[0089] The blaze criterion of the diffraction order can be further summarized as: μ = |m%1-0.5| (7)

[0090] That is, when μ approaches 0.5, m approaches an integer and reaches the blazing standard, and the spectrum at this level blazes; when μ approaches 0, m approaches an integer +0.5, and the light energy is dispersed over multiple levels of the spectrum.

[0091] According to the formula for the blazed grating, the one-dimensional diffraction simulation results for the first light L11 (532nm) and the second light L12 (640nm) are shown in Figures 8 and 9 (for symmetry, the diffraction along the X and Y axes has the same characteristics; the diffraction effect along one axis is used as an example here). The blaze order is calculated based on the blaze angle as follows:

[0092] λ=532nm,θ i =-16° Achieve spectral brilliance.

[0093] λ=640nm,θ i =-31° Achieving spectral brilliance

[0094] Therefore, the first diffracted light and the second diffracted light have different blazing orders, namely diffraction order 3 and diffraction order 4, respectively. The calculated results are consistent with the experimental data in Figures 8 and 9. When the first diffracted light (order 4) and the second diffracted light (order 3) blaze at the same time, the incident angles of the first light L11 and the second light L12 when entering the structured light generating element 31 are 16 and 31 degrees respectively. The diffraction angles θ of the blazing orders of the first diffracted light and the second diffracted light are θ. r' are 40.1 and 28.1 degrees respectively (calculation process is as follows). This also leads to the problem that when the excitation light of the first diffraction light and the second diffraction light wavelengths meet the blaze conditions at the same time, the diffraction angles of the target diffraction order are different due to the different wavelengths and diffraction orders, so the light spots of the target diffraction order cannot overlap. θ' green_r =2×8.55°+11°=28.1° θ′ red_r =2×8.55°+23°=40.1°

[0095] In order to ensure that the target diffraction orders in the first diffraction light and the second diffraction light can coincide, it is necessary to correct the diffraction angles of the third diffraction order of the first diffraction light and the fourth diffraction order of the second diffraction light L33 to be consistent.

[0096] Table 1 calculates the incident angles of the first light L11 and the second light L12 corresponding to the third order of diffraction of the first diffracted light and the fourth order of diffraction of the second diffracted light when entering the structured light generating element 31 under different diffraction angles.

[0097] Please refer to Figure 10. In Figure 10, the horizontal axis represents the diffraction angle, and the vertical axis represents the incident angle. The first light L11 and the first diffracted light correspond to curve R, and the second light L12 and the second diffracted light correspond to curve G. According to Figure 10, when ensuring that the diffraction angles of the target diffraction order are the same, the angle difference that the first light L11 and the second light L12 need to achieve varies slightly at different diffraction angles, and is approximately 2.1-2.2 degrees. The above angle difference is the angle difference that the angle adjustment component 20 in this application needs to achieve when the first light L11 and the second light L12 enter the structured light generating element 31.

[0098] Table 1

[0099] When the blaze conditions are met simultaneously, the incident angles of the first light L11 and the second light L12 are 16° and 31°, respectively, with an angle difference of 15°. However, when the diffraction angles are the same, the angle difference between the incident angles of the first light L11 and the second light L12 when entering the structured light generating element 31 must be 2.1°. Therefore, it is difficult to simultaneously meet the optimal blaze conditions for the first and second diffracted lights. Therefore, Table 1 records the incident angles and blaze criteria μ that the first and second diffracted lights should follow, assuming that the third and fourth orders of diffraction of the first and second diffracted lights are reflected at the same diffraction angle.

[0100] According to Table 1, when the incident angle of the first light L11 entering the structured light generating element 31 is 22.500° and the incident angle of the second light L12 entering the structured light generating element 31 is 24.635°, the blaze conditions are 0.353 and 0.341 respectively, so that the blaze conditions of the first diffracted light and the second diffracted light simultaneously reach the local optimal value, and the energy utilization rate of the target diffraction order reaches a relatively optimized level.

[0101] In the biometric recognition device 100 of this embodiment, in order to ensure that the blaze order (i.e., diffraction order 3) of the first diffraction light emitted from the structured light generating element 31 and the blaze order (i.e., diffraction order 4) of the second diffraction light coincide with each other, the angle adjustment component 20 is used to adjust the incident angles of the first light L11 and the second light L12 incident on the structured light generating element 31.

[0102] Please refer to Figures 11 and 12 together (to simplify the drawings, the reflectors 16, 25, and 26 in Figure 12 are not shown in Figure 2 above; in other embodiments of the present application, the reflectors 16, 25, and 26 may not be included in the optical path). The mixed light of the first light L11 and the second light L12 is incident on the prism 21. Since the refractive index n of the air and the prism (optical glass) 21 is different, according to Snell's law, the first light L11 and the second light L12 are refracted at the interface between the two media with different refractive indices. Then, the first light L11 and the second light L12 are refracted twice when passing through the prism 21 (once on the light receiving surface 211 and once on the light emitting surface 212 respectively). Based on the principle that the refractive index changes according to the wavelength of light, the first light L11 has an exit angle θ2 when it is emitted from the prism 21 after being refracted twice, and the second light L12 has an exit angle θ when it is emitted from the prism 21 after being refracted twice. 2g , thereby achieving the target angle difference of 2.1° required when the first light L11 and the second light L12 are incident on the structured light generating element 31.

[0103] Taking the first light L11 as an example, the incident angle θ1 and the exit angle θ2 satisfy the above formulas (8)-(9), where n represents the refractive index of the prism. As can be seen from the above derivation and calculation process, the target diffraction order of the first diffracted light and the second diffracted light, and the angular difference when the first light L11 and the second light L12 are incident on the structured light generating element 31 are all closely related to the wavelengths of the first light L11 and the second light L12. When the first light L11 and the second light L12 are lights of other wavelengths, the calculated target diffraction order and angular difference may be different. In addition, in other embodiments of the present application, the light source assembly 10 can output a larger number of lights of different wavelengths. In this case, the required angular difference between each two lights of different wavelengths and the target diffraction order of the diffracted light generated based on the lights of each different wavelength can be calculated according to the above calculation process.

[0104] Theoretically, because the first light L11 and the second light L12 have different exit angles when emitted from the prism 21, the first light L11 and the second light L12 will propagate away from the main optical axis according to their respective exit angles after exiting the prism 21. At this time, if the first light L11 and the second light L12 propagate through air and directly enter the structured light generating element 31, the light spots of the first light L11 and the second light L12 will not overlap on the surface of the structured light generating element 31, which may easily reduce the effective illumination area of ​​the structured light L2 and reduce the light energy utilization rate.

[0105] Therefore, the angle adjustment component 20 of this embodiment also uses the 4f optical conjugate system composed of the third lens 22 and the fourth lens 23 to make the light-receiving surface 211 of the prism 21 and the surface of the structured light generating element 31 reflecting the structured light L2 in an optical conjugate relationship. Because the light spots of the first light L11 and the second light L12 coincide on the light-receiving surface 211 of the prism 21 (the light incident on the light-receiving surface 211 is a mixture of the first light L11 and the second light L12, with uniform light intensity, parallel light beams, and overlapping light spots), the light spots of the first light L11 and the second light L12 can still remain overlapping on the conjugate surface. At the same time, it can also ensure that the incident angles of the first light L11 and the second light L12 incident on the structured light generating element 31 differ by 2.1°.

[0106] The spectral splitting effect of the first light L11 and the second light L12 is illustrated in the simulation results of Figures 13 and 14 . Figure 13 shows the overlap of the light spots (red light spot S1 and green light spot S2) on the spectral plane, while Figure 14 shows the overlap of the light spots on the modulation surface 312 of the structured light generating element 31 and its conjugate plane. As can be seen from Figures 13 and 14 , light beams of different wavelengths can be separated on the back focal plane (spectral plane) of the third lens 22, while the light spots of the first light L11 and the second light L12 substantially overlap on the surface of the structured light generating element 31 that reflects the structured light L2.

[0107] The biometric identification device 100 of this embodiment uses a three-step phase shift detection method. That is, the biometric identification device 100 operates in multiple detection cycles. In each detection cycle, the biometric identification device 100 collects six images of the sample 200 to be tested. In this embodiment, in each detection cycle, the phase is modulated successively. Phase Phase The structured light L2 can form a vertical stripe pattern to illuminate the sample 200 to collect three images respectively, and then modulate the phase Phase Phase The structured light L2 can form a horizontal stripe pattern to illuminate the sample 200 to collect three images respectively, and a total of six images are collected in each detection cycle. The controller 50 can perform super-resolution reconstruction based on the six images to obtain base sequence information of the sample 200.

[0108] By modulating six types of structured light L2 and completing a scan respectively, six images of the sample to be tested 200 can be collected. The time period for collecting six images corresponds to one detection cycle mentioned above. When the structured light L2 irradiates the sample to be tested 200, the fluorescent substance in the sample to be tested 200 is stimulated to produce fluorescence (defined as detection light L3 in this embodiment). Different fluorescent substances on the sample to be tested 200 can be stimulated to produce detection light L3 of different wavelengths. Therefore, in this embodiment, the detection light L3 includes two different wavelengths of light, the first detection light L31 and the second detection light L32.

[0109] In this embodiment, the imaging assembly 40 includes a first imaging channel 41 and a second imaging channel 42. The first imaging channel 41 is used to receive the first detection light L31 to capture an image, and the second imaging channel 42 is used to receive the second detection light L32 to capture an image. In other embodiments, the imaging assembly 40 may also include four imaging channels, for example.

[0110] The first imaging channel 41 includes a filter 411, a tube lens 412, and a camera 413, which are sequentially arranged on the optical path of the first detection light L31. The second imaging channel 42 includes a filter 421, a tube lens 422, and a camera 423, which are sequentially arranged on the optical path of the second detection light L32. The first imaging channel 41 and the second imaging channel 42 have substantially the same structure and function. The following description will use the first imaging channel 41 as an example.

[0111] The filter 411 is used to filter out light of wavelengths other than the first detection light L31 , and the tube lens 412 is used to focus the first detection light L31 onto the camera 413 . The camera 413 is used to capture an image based on the first detection light L31 .

[0112] In this embodiment, during the process of the structured light L2 scanning the sample to be tested 200, the sample to be tested 200 continues to generate detection light L3, and the cameras 413 and 423 are continuously in an exposure state to respectively capture images of the sample to be tested 200 according to the first detection light L31 and the second detection light L32, and synchronously end the exposure when the structured light L2 scan ends.

[0113] The imaging assembly 40 also includes an objective lens 43 and a spectrometer 44. The objective lens 43 is located between the light modulation assembly 30 and the sample 200 to be measured, and is used to project the structured light L2 onto the sample 200 to be measured. The spectrometer 44 is located between the sample 200 to be measured and the first imaging channel 41 and the second imaging channel 42. It is used to receive the detection light L3, reflect the first detection light L31 in the detection light L3, and transmit the second detection light L32 in the detection light L3 to achieve spectroscopic separation of the first detection light L31 and the second detection light L32. In this embodiment, the spectrometer 44 is a dichroic mirror.

[0114] The biometric recognition device 100 also includes a light guiding element 60 for guiding the propagation of a light beam. In this embodiment, the light guiding element 60 is a dichroic mirror located in the optical path of the structured light L2 and the detection light L3. It is used to reflect the structured light L2 from the light modulation assembly 30 onto the sample 200 to be tested and to transmit the detection light L3 from the sample 200 to the beam splitter 44.

[0115] In this embodiment, the surface of the structured light generating element 31 that emits the structured light L2 and the image plane of the objective lens 43 are conjugate surfaces to each other, which enables the structured light L2 to be focused on the back focal plane of the objective lens 43. The structured light L2 emitted by the objective lens 43 is a parallel light beam, and wide-field structured light illumination is realized at the front focal plane (image plane) of the objective lens 43. The striped structured light L2 generated by the light modulation component 30 can be scaled proportionally to the object plane and ensure that the phase information is the same.

[0116] In this embodiment, the surface of the sample to be tested 200 and the image planes of cameras 413 and 423 are conjugate surfaces to each other, so that the first detection light L31 can be received by the exposure surface of camera 413, and the second detection light L32 can be received by the exposure surface of camera 423, which is beneficial to improving the utilization rate of light energy.

[0117] In this embodiment, the controller 50 includes a signal transmitter, an image acquisition card, and a computer. The computer transmits drive signals through the signal transmission controller to drive the first light-emitting element 11, the second light-emitting element 12, the structured light generating element 31, the camera 413, and the camera 423. This controls the on / off switching of the first light-emitting element 11 and the second light-emitting element 12, the modulation process of the structured light generating element 31, and the exposure time of the cameras 413 and 423. The computer is also used to perform super-resolution reconstruction on the image captured by the imaging component 40 to obtain a super-resolution image, and to obtain base sequence information of the sample 200 based on the super-resolution image.

[0118] In the embodiment of the present application, through the simulation of the physical model of the blazed grating and the angle calculation, it was found that the first diffraction light and the second diffraction light generated based on the first light L11 and the second light L12 respectively have different blazed angles and corresponding different blazed diffraction orders. If the first light L11 and the second light L12 are incident on the structured light generating element 31 at the same angle, it is difficult for the target diffraction order (blazed order) of the first diffraction light and the target diffraction order (blazed order) of the second diffraction light to coincide with each other, which is not conducive to the utilization of light energy. Therefore, in order to improve the utilization rate of light energy, the above-mentioned biometric recognition device 100 and wide-field lighting system must make the target diffraction order (blazed order) of the first diffraction light and the target diffraction order (blazed order) of the second diffraction light coincide with each other, which requires ensuring that the first light L11 and the second light L12 meet the blazed conditions at the same time.

[0119] Through physical model simulation and angle calculation, in this embodiment, when the wavelength of the first light L11 is 630nm and the wavelength of the second light L12 is 532nm, when the incident angle of the first light L11 incident on the structured light generating element 31 is -31° and the incident angle of the second light L12 incident on the structured light generating element 31 is -16°, the third diffraction order of the first diffraction light and the fourth diffraction order of the second diffraction light blaze simultaneously. The angular difference between the diffraction angles corresponding to the third diffraction order of the first diffraction light and the fourth diffraction order of the second diffraction light is 12°. The present application uses the angle adjustment component 20 to split the first light L11 and the second light L12 so that the incident angles of the first light L11 and the second light L12 incident on the structured light generating element 31 have an angular difference of 2.1°, so as to compensate for the 12° diffraction angle difference between the third diffraction order of the first diffraction light and the fourth diffraction order of the second diffraction light, so that the third diffraction order of the first diffraction light and the fourth diffraction order of the second diffraction light overlap.

[0120] In a pair of comparisons, due to the diffraction effect of the diffraction element, the target diffraction orders of light of different wavelengths cannot overlap, resulting in a limited energy utilization rate of the target diffraction orders, typically less than 5%, and causing uneven energy utilization of light of different wavelengths. However, the present application uses the angle adjustment component 20 to compensate for the difference in diffraction angle between the first diffracted light and the second diffracted light, allowing the target diffraction orders of the first diffracted light and the second diffracted light to overlap. The target diffraction orders of the first diffracted light and the second diffracted light can be simultaneously utilized by the biometric recognition device 100, which can more than double the light energy utilization rate of the biometric recognition device 100 and optimize the imaging efficiency of the biometric recognition device 100.

[0121] When the target diffraction orders of the first and second diffracted lights coincide, they can propagate along the principal optical axis and enter the back pupil of the objective lens 43 along the principal optical axis. This ensures that the target diffraction orders can simultaneously enter the back pupil of the objective lens 43 and participate in the generation of the fringe structured light L2, effectively improving the fringe contrast and the contrast and signal-to-noise ratio of the biometric recognition device 100. Furthermore, the central field of view of the objective lens 43 can be fully utilized, ensuring high imaging quality while also achieving high imaging accuracy for the first detection light L31 and the second detection light L32 of different wavelengths.

[0122] In addition, the angle adjustment component 20 also includes a 4f lens group, so that the light receiving surface 211 of the prism 21 and the modulation surface 312 of the structured light generating element 31 are in a conjugate relationship, so that the incident angles of the first light L11 and the second light L12 entering the structured light generating element 31 maintain an angle difference of 2.1°, and at the same time, the light spots of the first light L11 and the second light L12 on the modulation surface 312 overlap. The first light L11 and the second light L12 can be utilized to a large extent by the structured light generating element 31, avoiding the waste of light energy and further improving the light utilization rate.

[0123] Furthermore, the present application utilizes a single optical path (the optical path formed by the prism 21 and the 4f lens group) to split the first light L11 and the second light L12, adjusting the incident angle when entering the structured light generating element 31. This simplifies the optical path, reduces the cost of optical components, and is easy to implement. This avoids the use of expensive optical components and the use of a complex optical path structure.

[0124] Furthermore, embodiments of the present application also relate to a method for identifying biological samples. As shown in FIG15 , the method is preferably performed using the aforementioned biometric identification device 100, and includes: a light source assembly 10 emitting a first light and a second light of different wavelengths; directing the first light and the second light through a prism 21 to emit at different angles; receiving the first light and the second light after emitting from the prism by a structured light generating element 31, modulating the intensity and phase of the received first light and the second light to generate structured light, the structured light including a first diffracted light formed based on the first light and a second diffracted light formed based on the second light; wherein the first light and the second light from the prism 21 are incident on the structured light generating element 31 at different incident angles so that the target diffraction orders of the first diffracted light and the second diffracted light coincide; the structured light irradiates a sample to be tested to generate detection light, and receiving the detection light to generate an image; and detecting information about the biological sample based on the image recognition.

[0125] Optionally, detecting the biological sample information based on the image recognition includes: performing super-resolution reconstruction on the image to generate a super-resolution image, and acquiring the biological information of the sample to be tested based on the super-resolution image.

[0126] In an optional embodiment of the biological sample identification method of the present application, the light source assembly 10 described above is used to emit a combined light of the first light L11 and the second light L12. The prism 21 is preferably a triangular prism and is located in the optical paths of the first light L11 and the second light L12. The prism 21 is used to change the propagation directions of the first light L11 and the second light L12, respectively, to split the first light L11 and the second light L12, so that the first light L11 and the second light L12 are emitted from the prism 21 at different angles.

[0127] In an optional embodiment of the biological sample identification method of the present application, the structured light generating element 31 may be a digital micromirror device (DMD). In other embodiments of the present application, the structured light generating element 31 may be other dimming devices, such as a liquid crystal spatial light modulator (LCSLM), a deformable mirror, a microlens array, or other digital gratings, or a traditional physical grating. The above-mentioned digital grating and physical grating also have a diffraction effect on the first light L11 and the second light L12 when modulating the first light L11 and the second light L12. The specific process of performing the biological sample identification method using the above-mentioned biometric identification device 100 is similar to the operation process of the various components of the above-mentioned biometric identification device 100, and will not be described in detail here.

[0128] Those skilled in the art should recognize that the above embodiments are merely intended to illustrate the present application and are not intended to limit the present application. As long as they are within the spirit of the present application, appropriate changes and modifications to the above embodiments are within the scope of protection claimed in the present application.

Claims

1. A wide-field lighting system, characterized in that: include: A light source assembly, configured to emit a first light and a second light having different wavelengths; An angle adjustment component, comprising a prism located on the optical path of the first light and the second light, wherein the prism emits the received first light and the second light at different angles; A light modulation component, comprising a structured light generating element located on the optical path of the first light and the second light, wherein the structured light generating element can modulate the intensity and phase of the received first light and the second light to generate structured light, wherein the structured light is used to illuminate the sample to be tested to form an image; Among them, the structured light includes a first diffraction light formed based on the first light and a second diffraction light formed based on the second light, and the first light and the second light from the prism are incident on the structured light generating element at different incident angles so that the target diffraction order of the first diffraction light coincides with the target diffraction order of the second diffraction light.

2. The wide field illumination system according to claim 1, characterized in that: The prism has a light receiving surface and a light emitting surface, the light receiving surface intersects with the light emitting surface, the first light and the second light are incident on the light receiving surface in parallel, and are emitted from the light emitting surface at different angles after being refracted twice by the prism.

3. The wide field illumination system according to claim 2, characterized in that: The light receiving surface is in a conjugate relationship with a surface of the structured light generating element that receives the first light and the second light.

4. The wide field illumination system according to claim 1, characterized in that: The angle adjustment assembly further includes a 4f lens group located between the prism and the structured light generating element; The 4f lens group guides the first light and the second light from the prism to the structured light generating element, so that the light spots formed by the first light and the second light on the structured light generating element overlap.

5. The wide field illumination system according to claim 1, characterized in that: The structured light generating element is a physical grating or a digital grating.

6. The wide field illumination system according to claim 5, characterized in that: The structured light generating element is a digital micro-mirror device.

7. The wide-field illumination system according to any one of claims 1 to 6, characterized in that: The target diffraction orders of the first diffracted light and the second diffracted light are blaze orders.

8. The wide field illumination system according to claim 7, characterized in that: The first light is red light, the second light is green light, the angle difference between the incident angles of the first light and the second light incident on the structured light generating element is 2.1°, the target diffraction order of the first diffracted light is diffraction order 3, and the target diffraction order of the second diffracted light is diffraction order 4.

9. The wide field illumination system according to claim 1, wherein: The target diffraction orders include diffraction orders 0 and ±1.

10. A biological sample identification method, characterized in that: include: The light source assembly emits a first light and a second light having different wavelengths; The light paths of the first light and the second light are emitted through a prism at different angles; The first light and the second light emitted from the prism are received by a structured light generating element, and the structured light generating element modulates the light intensity and phase of the received first light and the second light to generate structured light, wherein the structured light includes a first diffracted light formed based on the first light and a second diffracted light formed based on the second light; wherein the first light and the second light from the prism are incident on the structured light generating element at different incident angles, so that the target diffraction order of the first diffracted light and the target diffraction order of the second diffracted light coincide with each other; The structured light generates detection light after irradiating the sample to be tested, and the detection light is received to generate an image; and The information of the biological sample is detected based on the image recognition.

11. The biological sample identification method according to claim 10, characterized in that: The detecting the biological sample information based on the image recognition includes: Super-resolution reconstruction is performed according to the image to generate a super-resolution image, and biological information of the sample to be tested is acquired based on the super-resolution image.

12. The biological sample identification method according to claim 10, characterized in that: The prism has a light receiving surface and a light emitting surface, the light receiving surface intersects with the light emitting surface, the first light and the second light are incident on the light receiving surface in parallel, and are emitted from the light emitting surface at different angles after being refracted twice by the prism.

13. The biological sample identification method according to claim 12, characterized in that: The light emitting surface is in a conjugate relationship with a surface of the structured light generating element that receives the first light and the second light.

14. The biological sample identification method according to claim 10, characterized in that: The angle adjustment assembly further includes a 4f lens group located between the prism and the structured light generating element; The 4f lens group guides the first light and the second light from the prism to the structured light generating element, so that the light spots formed by the first light and the second light on the structured light generating element overlap.

15. The biological sample identification method according to claim 11, characterized in that: The structured light generating element is a physical grating or a spatial light modulator.

16. The biological sample identification method according to claim 15, characterized in that: The structured light generating element is a digital micro-mirror device.

17. The biological sample identification method according to claim 10, characterized in that: The target diffraction orders of the first diffracted light and the second diffracted light are blaze orders.

18. The biological sample identification method according to claim 17, characterized in that: The first light is red light, the second light is green light, the angle difference between the incident angles of the first light and the second light incident on the structured light generating element is 2.1°, the target diffraction order of the first diffracted light is diffraction order 3, and the target diffraction order of the second diffracted light is diffraction order 4.

19. The biological sample identification method according to claim 10, characterized in that: The target diffraction orders include diffraction orders 0 and ±1.

20. The biological sample identification method according to claim 10, characterized in that: An objective lens is provided between the structured light generating element and the sample to be measured; The first diffracted light of the target diffraction order and the second diffracted light of the target diffraction order are incident on the objective lens along the main optical axis of the objective lens.

21. The biological sample identification method according to claim 20, characterized in that: A surface of the structured light generating element receiving the first light and the second light is in a conjugate relationship with a phase plane of the objective lens.

22. The biological sample identification method according to claim 20, characterized in that: A camera is used to receive the detection light to generate an image; the object plane of the objective lens is in a conjugate relationship with the surface of the camera that receives the detection light.

23. The biological sample identification method according to any one of claims 10 to 21, characterized in that: The detection light generated by the first diffracted light and the second diffracted light is detected by different imaging channels respectively to collect images respectively.

24. The biological sample identification method according to any one of claims 10 to 21, characterized in that: The sample to be tested is a nucleic acid sample, and the biological information is the base sequence information of the sample to be tested.

25. A sequencing device, characterized in that: The sequencing device comprises: A light source assembly, configured to emit a first light and a second light having different wavelengths; An angle adjustment component, comprising a prism located on the optical path of the first light and the second light, wherein the prism emits the received first light and the second light at different angles; a light modulation component, comprising a structured light generating element located on an optical path of the first light and the second light, wherein the structured light generating element can modulate the light intensity and phase of the received first light and the second light to generate structured light, wherein the structured light includes a first diffracted light formed based on the first light and a second diffracted light formed based on the second light, and the first light and the second light from the prism are incident on the structured light generating element at different incident angles, so that target diffraction orders of the first diffracted light and the second diffracted light coincide with each other; a sample stage, the sample stage being used to support a sample to be tested, the sample to be tested receiving the first diffracted light and the second diffracted light and generating detection light, an imaging assembly including a camera that receives the detection light and generates an image; A controller is electrically connected to the camera and is used to perform super-resolution reconstruction according to the image to generate a super-resolution image, and to obtain base sequence information of the sample to be tested according to the super-resolution image.

26. The sequencing device according to claim 25, characterized in that The prism has a light receiving surface and a light emitting surface, the light receiving surface intersects with the light emitting surface, the first light and the second light are incident on the light receiving surface in parallel, and are emitted from the light emitting surface at different angles after being refracted twice by the prism.

27. The sequencing device according to claim 26, characterized in that The light emitting surface is in a conjugate relationship with a surface of the structured light generating element that receives the first light and the second light.

28. The sequencing device according to claim 26, characterized in that The angle adjustment assembly further includes a 4f lens group located between the prism and the structured light generating element; The 4f lens group guides the first light and the second light from the prism to the structured light generating element, so that the light spots formed by the first light and the second light on the structured light generating element overlap.

29. The sequencing device according to claim 25, characterized in that The structured light generating element is a physical grating or a spatial light modulator.

30. The sequencing device according to claim 29, wherein: The structured light generating element is a digital micro-mirror device.

31. The sequencing device according to claim 25, characterized in that The target diffraction orders of the first diffracted light and the second diffracted light are blaze orders.

32. The sequencing device according to claim 31, characterized in that The first light is red light, the second light is green light, the angle difference between the incident angles of the first light and the second light incident on the structured light generating element is 2.1°, the target diffraction order of the first diffracted light is diffraction order 3, and the target diffraction order of the second diffracted light is diffraction order 4.

33. The sequencing device according to claim 25, wherein: The target diffraction orders include diffraction orders 0 and ±1.

34. The sequencing device according to claim 25, wherein: The imaging assembly further includes an objective lens located between the structured light generating element and the camera; The first diffracted light of the target diffraction order and the second diffracted light of the target diffraction order are incident on the objective lens along the main optical axis of the objective lens.

35. The sequencing device according to claim 34, wherein: A surface of the structured light generating element receiving the first light and the second light is in a conjugate relationship with a phase plane of the objective lens.

36. The sequencing device according to claim 34, wherein: The object plane of the objective lens is in a conjugate relationship with the surface of the camera receiving the detection light.

37. The sequencing device according to any one of claims 25 to 36, characterized in that: The imaging component includes a first imaging channel and a second imaging channel, and the first imaging channel and the second imaging channel are used to respectively receive detection light generated based on the first diffracted light and the second diffracted light to respectively collect images.

Images