Reagent cartridge and sample testing apparatus

Abstract

A reagent cartridge and a sample testing apparatus, relating to the technical field of nucleic acid testing. The reagent cartridge comprises a cartridge body, a reaction tube and a driving member, the cartridge body comprising a driving chamber, a rotary valve, an elution chamber and multiple accommodating chambers, the driving chamber, and the rotary valve, the elution chamber and the multiple accommodating chambers being connected in sequence and integrated into one body; the reaction tube is connected to the driving chamber, and the reaction tube is in communication with the driving chamber; a first flow path is provided on a side wall of the elution chamber, and a second flow path is provided on a side wall of the driving chamber; the rotary valve is configured to cause the first flow path and the second flow path to be open during rotation of the valve; the driving member is movably disposed in the driving chamber, the driving member being configured to form a negative pressure in the driving chamber and the reaction tube during movement relative to the driving chamber when the first flow path and the second flow path are in communication, so that a liquid in the elution chamber enters the reaction tube by means of, sequentially, the second flow path, the rotary valve and the first flow path.

Description

Reagent cartridge and sample detection device

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority to Chinese patent application number 202311250805.7, filed with the Patent Office of China on September 25, 2023, entitled “A Reagent Cartridge and Sample Detection Device,” the entire contents of which are incorporated by reference into this application. Technical Field

[0003] The present disclosure relates to the technical field of nucleic acid detection, and in particular to a reagent cartridge and a sample detection device. Background Art

[0004] Currently, there are a variety of molecular products on the market developed based on microfluidic chip technology. Most of them achieve liquid transfer between different chambers by creating negative pressure or centrifugation. In addition, the liquid transfer part of most products is achieved by moving the on-off parts in the vertical direction to achieve the opening and closing of the liquid flow path. Due to the small aperture of the flow path, this structure places higher requirements on the driving accuracy of the movement position, which is not conducive to the flexible, convenient, and accurate control of the liquid channel. In addition, the driving direction of the reagent cartridge is located in different positions, which is not conducive to the integration of the reagent cartridge drive mechanism. In addition, most of the structures that control the opening and closing of the liquid channel are complex, which in turn makes the reagent cartridge structure more complex and the production cost higher.

[0005] Summary of the Invention

[0006] The present disclosure provides a reagent cartridge and a sample detection device, which can conveniently and accurately control the on-off of a liquid channel, thereby realizing the transfer of liquid between different chambers, and simplifies the structure of the reagent cartridge and reduces production costs.

[0007] The embodiments of the present disclosure may be implemented as follows:

[0008] An embodiment of the present disclosure provides a reagent cartridge comprising:

[0009] The box body includes a driving chamber, a rotary valve, an elution chamber and a plurality of accommodating chambers, which are sequentially connected and integrated into one body;

[0010] A reaction tube is connected to the driving chamber, and the reaction tube communicates with the driving chamber;

[0011] A first flow path is provided on the side wall of the elution cavity, and a second flow path is provided on the side wall of the driving cavity;

[0012] The rotary valve is configured to connect or block the first flow path and the second flow path during rotation;

[0013] The driving member is movably disposed in the driving chamber. The driving member is configured to form a negative pressure in the driving chamber and the reaction tube during movement relative to the driving chamber when the first flow path and the second flow path are connected, so that the liquid in the elution chamber enters the reaction tube through the second flow path, the rotary valve, and the first flow path in sequence.

[0014] In an optional embodiment, the rotary valve includes a valve chamber, a valve stem, and a swinging member, wherein an upper portion of the valve stem is connected to the swinging member, a through hole is formed in the valve stem, and a lower portion of the valve stem is located in the valve chamber, and the swinging member is configured to rotate the valve stem so that the valve stem can rotate relative to the valve chamber;

[0015] The cavity openings of the valve cavity, the driving cavity, the elution cavity and the plurality of accommodating cavities are all located on the upper end surface of the box body.

[0016] In an optional embodiment, the valve cavity is provided with a first limit block and a second limit block along the rotation path of the swing member, and the first limit block and the second limit block are configured to limit the swing stroke of the swing member.

[0017] In an optional embodiment, when the swing member is located at the position of the first limit block, the first flow path and the second flow path are connected.

[0018] In an optional embodiment, when the swing member rotates to the first limit block, the rotary valve is in the first position, and the through hole connects the first flow path to the second flow path;

[0019] When the swinging member is located between the first limiting block and the second limiting block and is not in contact with the first limiting block, the rotary valve is in the second position, and the rotary valve blocks the first flow path and the second flow path.

[0020] In an optional embodiment, the valve stem includes a first valve stem portion, a second valve stem portion, and a connecting portion, wherein one end of the first valve stem portion is connected to the swing member, the other end of the first valve stem portion is connected to one end of the second valve stem portion, and the connecting portion is connected to the other end of the second valve stem portion;

[0021] The first valve stem portion comprises two cross-intersecting plate-shaped structures, and the second valve stem portion is provided with a through hole.

[0022] In an optional embodiment, the first valve stem portion is provided with annular protrusions at intervals along the extension direction of the first valve stem portion, and the annular protrusions are configured to be in close contact with the valve cavity to prevent the first valve stem portion and the second valve stem portion from tilting during rotation.

[0023] In an optional embodiment, the connecting portion includes a plurality of claws, one end of each claw being connected to the second valve stem portion, and the other end of the claw being provided with a limiting protrusion; the plurality of claws are evenly distributed along the circumference of the second valve stem, with a gap between adjacent claws; the plurality of claws are all made of an elastic material, and the plurality of claws are retractable;

[0024] A protrusion is provided in the valve cavity. When the limiting protrusion passes through the protruding position, the multiple claws retract inward; when the limiting protrusion passes through the protruding position, the multiple claws rebound, thereby achieving fixed limiting of the rotary valve and the valve cavity.

[0025] In an optional embodiment, the end of the claw away from the valve stem portion is a guide arc surface.

[0026] In an optional embodiment, the second flow path includes a horizontal channel and a vertical channel, the horizontal channel is located at the same horizontal plane as the first flow path, and the vertical channel is configured to connect the reaction tube and the horizontal channel.

[0027] In an optional embodiment, a blocking member is provided at one end of the horizontal channel away from the rotary valve.

[0028] In an optional embodiment, the multiple accommodating chambers are a first cleaning chamber, a second cleaning chamber, a magnetic bead chamber, a sample chamber and a magnetic rod sleeve chamber, and the first cleaning chamber, the second cleaning chamber, the magnetic bead chamber, the sample chamber and the magnetic rod sleeve chamber are arranged in sequence from the end of the box body close to the elution chamber to the end of the box body away from the elution chamber.

[0029] In an optional embodiment, forming holes are opened in radial positions of partial flow sections of the first flow path and the second flow path.

[0030] An embodiment of the present disclosure further provides a sample detection device, comprising a detection component and any of the above-mentioned reagent cartridges, wherein the reagent cartridge is connected to the detection component.

[0031] In an optional embodiment, the detection assembly includes a detection platform, a motion mechanism, a magnetic rod sleeve grasping mechanism and a toggle member, and the detection platform is configured to place a reagent cartridge;

[0032] The motion mechanism is connected to the magnetic rod cover grabbing mechanism and the toggle member respectively. The motion mechanism is located above the detection platform and drives the magnetic rod cover grabbing mechanism and the toggle member to move above the reagent cartridge.

[0033] The magnetic rod sleeve grabbing mechanism is configured to grab the magnetic rod for sample testing; the toggle member is configured to rotate the rotary valve;

[0034] The motion mechanism is provided with a groove, which is configured to clamp the driving member. The motion mechanism moves up and down, so that the driving member moves relative to the driving cavity.

[0035] In an optional embodiment, the detection assembly further includes a movable member, and the movable member is configured to drive the driving member to move relative to the driving cavity.

[0036] The beneficial effects of the reagent cartridge and sample detection device according to the disclosed embodiments include:

[0037] By using a rotary valve to control the connection and disconnection between the first and second flow paths, liquid transfer between different chambers is achieved, making it possible to conveniently and accurately control the connection and disconnection between the first and second flow paths. At the same time, multiple cavities are provided on the cartridge body, and the rotary valve that controls the connection and disconnection between the first flow path in the elution chamber and the second flow path in the drive chamber is set in one of the cavities of the cartridge body, simplifying the structure of the reagent cartridge and reducing production costs. Moreover, both the drive element and the rotary valve are located above the cartridge body, making it convenient to set a moving mechanism above the reagent cartridge to drive the movement of the drive element and the rotary valve, facilitating the integration of the sample detection device. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the following briefly introduces the drawings required for use in the embodiments. It should be understood that the following drawings only illustrate certain embodiments of the present disclosure and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other relevant drawings can be obtained based on these drawings without any creative work.

[0039] FIG1 is a schematic diagram of a sample detection device provided in an embodiment of the present disclosure;

[0040] FIG2 is a schematic diagram of a reagent cartridge provided in an embodiment of the present disclosure from a first viewing angle;

[0041] FIG3 is a schematic diagram of a reagent cartridge provided in an embodiment of the present disclosure from a second viewing angle;

[0042] FIG4 is a schematic diagram of an AA cross section provided in an embodiment of the present disclosure;

[0043] FIG5 is a schematic diagram of a BB cross section provided in an embodiment of the present disclosure;

[0044] FIG6 is a schematic diagram of a rotary valve provided in an embodiment of the present disclosure.

[0045] Icons: 1-sample detection device; 1000-reagent cartridge; 100-cartridge body; 110-driving chamber; 111-second flow path; 1111-horizontal channel; 1112-vertical channel; 120-rotary valve; 121-valve chamber; 1211-first stopper; 1212-second stopper; 1213-protrusion; 122-valve stem; 1221-first valve stem portion; 12211-annular protrusion; 1222-second valve stem portion; 12221-through hole; 12222-annular groove; 1223-connecting portion; 12231-claw ;122311-limiting protrusion;122312-guide arc surface;123-swinging member;130-elution chamber;131-first flow path;140-accommodating chamber;141-first cleaning chamber;142-second cleaning chamber;143-magnetic bead chamber;144-sample chamber;145-magnetic rod sleeve chamber;200-reaction tube;300-driving member;400-sealing member;2000-detection component;2100-detection platform;2200-movement mechanism;2210-groove;2300-magnetic rod sleeve grasping mechanism;2400-sliding member. DETAILED DESCRIPTION

[0046] To make the objectives, technical solutions, and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present disclosure. Obviously, the described embodiments are only some of the embodiments of the present disclosure, but not all of them. Generally, the components of the embodiments of the present disclosure described and shown in the drawings herein can be arranged and designed in various different configurations.

[0047] Therefore, the following detailed description of the embodiments of the present disclosure provided in the accompanying drawings is not intended to limit the scope of the present disclosure as claimed, but merely represents selected embodiments of the present disclosure. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present disclosure without making any creative efforts shall fall within the scope of protection of the present disclosure.

[0048] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, it does not need to be further defined or explained in subsequent drawings.

[0049] In the description of the present disclosure, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear, the orientation or position relationship indicated is based on the orientation or position relationship shown in the accompanying drawings, or is the orientation or position relationship in which the invented product is usually placed when used. It is only for the convenience of describing the present disclosure and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation. Therefore, it cannot be understood as a limitation on the present disclosure.

[0050] In addition, the terms "first", "second", etc., if used, are merely used to distinguish and describe, and should not be understood as indicating or implying relative importance.

[0051] It should be noted that, in the absence of conflict, the features in the embodiments of the present disclosure may be combined with each other.

[0052] Currently, there are a variety of molecular POCT (Point of Care Testing) products on the market developed based on microfluidic chip technology. Most of them achieve liquid transfer between different chambers by creating negative pressure or centrifugation. In addition, the liquid transfer portion of most products is achieved by moving the on-off parts in the vertical direction to achieve the opening and closing of the liquid flow path. Due to the small aperture of the flow path, this structure places higher requirements on the driving accuracy of the motion position, which is not conducive to the flexible, convenient, and accurate control of the liquid channel. In addition, the driving direction of the reagent cartridge is located in different positions, which is not conducive to the integration of the reagent cartridge drive mechanism. In addition, most of the structures that control the opening and closing of the liquid channel are complex, which in turn makes the reagent cartridge structure more complex and the production cost higher.

[0053] Based on this, please refer to Figure 1. A reagent cartridge 1000 and a sample detection device 1 provided in an embodiment of the present disclosure can effectively improve the technical problems mentioned above. The reagent cartridge 1000 can conveniently and accurately control the opening and closing of the liquid channel, thereby realizing the transfer of liquid between different chambers, and simplifies the structure of the reagent cartridge 1000, reducing production costs. The reagent cartridge 1000 is applied to the sample detection device 1. The driving member 300 and the rotary valve 120 of the reagent cartridge 1000 are both located above the box body 100. Using the reagent cartridge 1000, it is convenient to set a motion mechanism 2200 above the reagent cartridge 1000 to drive the driving member 300 and the rotary valve 120 to move, thereby facilitating the integration of the sample detection device 1.

[0054] FIG1 is a schematic diagram of a sample detection device 1 provided in an embodiment of the present disclosure. As shown in FIG1 , the sample detection device 1 provided in an embodiment of the present disclosure includes a detection component 2000 and a reagent cartridge 1000 , and the reagent cartridge 1000 is connected to the detection component 2000 . Among them, the detection component 2000 provided in this embodiment includes a detection platform 2100, a motion mechanism 2200, a magnetic rod sleeve grasping mechanism 2300 and a toggle member 2400, and the detection platform 2100 is configured to place the reagent cartridge 1000; the motion mechanism 2200 is connected to the magnetic rod sleeve grasping mechanism 2300 and the toggle member 2400 respectively, and the motion mechanism 2200 is located above the detection platform 2100, and the motion mechanism 2200 drives the magnetic rod sleeve grasping mechanism 2300 and the toggle member 2400 to move above the reagent cartridge 1000; the magnetic rod sleeve grasping mechanism 2300 is configured to grasp the magnetic rod sleeve for sample detection; the toggle member 2400 is configured to rotate the rotary valve 120; the motion mechanism 2200 is provided with a groove 2210, and the groove 2210 is configured to clamp the driving member 300, and the motion mechanism 2200 moves up and down, so that the driving member 300 moves relative to the driving cavity 110. The magnetic rod sleeve is configured to adsorb magnetic beads and mix sample liquid for sample detection.

[0055] The aforementioned "movement mechanism 2200 defines a groove 2210, which is configured to retain the driver 300. The movement mechanism 2200 moves up and down, causing the driver 300 to move relative to the drive chamber 110. The magnetic rod sleeve is configured to attract magnetic beads and a sample solution for sample testing" may refer to the following: in this embodiment, the driver 300 is a piston. The movement mechanism 2200 moves in the X-direction, positioning the groove 2210 obliquely above the driver 300. The movement mechanism 2200 then moves in the Z-direction, toward the detection platform 2100, so that the groove 2210 and the upper portion of the piston are flush with each other. The movement mechanism 2200 then moves in the X-direction, engaging the groove 2210 with the piston. The movement mechanism 2200 then moves upward in the Z-direction, away from the detection platform 2100, to lift the piston upward. After testing is complete, the movement mechanism 2200 lowers the piston back into the drive chamber 110, and then moves to disengage the groove 2210 from the piston.

[0056] Of course, detection assembly 2000 may also include a movable member (not shown), which replaces the above-mentioned groove 2210 structure. The movable member is configured to drive a driver 300 so that the driver 300 moves relative to the drive chamber 110. The movable member drives the driver 300 in a manner similar to the motion mode of the above-mentioned groove 2210 structure, which will not be described in detail herein. The reagent cartridge 1000 is provided with a connecting structure, which is fixedly connected to the detection platform 2100 by the connecting structure. The motion mechanism 2200 above the detection platform 2100 can drive the magnetic rod cover gripping mechanism 2300, the toggle member 2400 and the movable member to move in three directions, X, Y, and Z, so that it moves above the reagent cartridge 1000 to complete the sample detection work. In the present embodiment, the motion mechanism 2200 mainly moves in the X and Z directions.

[0057] To save time and simultaneously test multiple reagent cartridges 1000, please continue to refer to Figure 1. Multiple reagent cartridges 1000 placement positions can be set on the testing platform 2100, and the motion mechanism 2200 can be provided with multiple grooves 2210 to connect multiple magnetic rod sleeve grasping mechanisms 2300 and multiple toggles 2400. The spacing between the multiple grooves 2210, the multiple magnetic rod sleeve grasping mechanisms 2300, and the multiple toggles 2400 is the same as the spacing between the multiple reagent cartridges 1000.

[0058] The structure of the reagent cartridge 1000 is described in detail below.

[0059] In order to facilitate the integration of the reagent cartridge 1000 and the integration of the driving mechanism of the sample detection device 1, please refer to Figures 2, 3, 4 and 5. Figure 2 is a schematic diagram of the reagent cartridge 1000 provided in the embodiment of the present disclosure from a first perspective; Figure 3 is a schematic diagram of Figure 2 from another perspective; Figure 4 is a schematic diagram of the A-A cross-section provided in the embodiment of the present disclosure; Figure 5 is a schematic diagram of the B-B cross-section provided in the embodiment of the present disclosure. The reagent cartridge 1000 provided in the embodiment of the present disclosure includes a cartridge body 100, a reaction tube 200 and a driving member 300. The cartridge body 100 includes a driving chamber 110, a rotary valve 120, an elution chamber 130 and a plurality of accommodating chambers 140. The driving chamber 110, the rotary valve 120, the elution chamber 130 and the plurality of accommodating chambers 140 are connected in sequence. The reaction tube 200 is connected to the drive chamber 110, and the reaction tube 200 is in communication with the drive chamber 110. A first flow path 131 is defined on the side wall of the elution chamber 130, and a second flow path 111 is defined on the side wall of the drive chamber 110. The rotary valve 120 is configured to connect or block the first flow path 131 and the second flow path 111 during rotation. The driving member 300 is movably disposed within the drive chamber 110. The driving member 300 is configured to create a negative pressure within the drive chamber 110 and the reaction tube 200 during movement relative to the drive chamber 110, when the first flow path 131 and the second flow path 111 are in communication. This allows the liquid in the elution chamber 130 to enter the reaction tube 200 through the second flow path 111, the rotary valve 120, and the first flow path 131 in sequence. The aforementioned "the reaction tube 200 is connected to the drive chamber 110" may refer to the reaction tube 200 being sealed to the drive chamber 110. The rotary valve 120 can be used to more conveniently control the connection and blocking of the first flow path 131 and the second flow path 111. At the same time, disposing the rotary valve 120 in one of the cavities of the box body 100 simplifies the structure of the reagent cartridge 1000.

[0060] Please refer to Figures 2, 3, 4, and 5, and in conjunction with Figure 6, which is a schematic diagram of a rotary valve 120 provided in an embodiment of the present disclosure. The driving member 300 provided in this embodiment is a piston. The rotary valve 120 in this embodiment includes a valve chamber 121, a valve stem 122, and a swinging member 123. The upper portion of the valve stem 122 is connected to the swinging member 123. The valve stem 122 is provided with a through hole 12221. The lower portion of the valve stem 122 is located in the valve chamber 121. The swinging member 123 is configured to rotate the valve stem 122 so that the valve stem 122 can rotate relative to the valve chamber 121. The cavity openings of the valve chamber 121, the driving chamber 110, the elution chamber 130, and the multiple accommodating chambers 140 are all located on the upper end surface of the box body 100. To facilitate the rotation of the swinging member 123 by the toggle member 2400, the swinging member 123 in this embodiment includes a main body and a rectangular convex strip. The toggle member 2400 contacts the convex strip. The toggle member 2400 rotates the swinging member 123, thereby rotating the valve stem 122 relative to the valve chamber 121. When the toggle member 2400 rotates the swinging member 123, thereby rotating the valve stem 122 relative to the valve chamber 121, the through hole 12221 connects the first flow path 131 and the second flow path 111. At this time, the movable member drives the piston to move upward relative to the drive chamber 110, connecting the reaction tube 200 with the drive chamber 110. A negative pressure is generated in the drive chamber 110, allowing the liquid in the elution chamber 130 to enter the reaction tube 200 through the second flow path 111, the through hole 12221 of the rotary valve 120, and the first flow path 131. Of course, the drive member 300 can also have other structures that can move relative to the drive chamber 110, and this is not limited here. The shape of the convex strip is not limited, as long as it is convenient for the shifting member 2400 to drive it to rotate.

[0061] In addition, the moving mechanism 2200 can also be used to make the toggle member 2400 contact the convex strip, and the moving mechanism 2200 can drive the toggle member 2400 to move along the X direction. When the moving mechanism 2200 drives the toggle member 2400 to a certain position, the through hole 12221 can also be connected to the first flow path 131 and the second flow path 111, thereby rotating the convex strip and driving the valve stem 122 to rotate relative to the valve cavity 121.

[0062] To control the rotation angle of rotary valve 120 and connect the first flow path 131 and the second flow path 111 without precisely controlling the rotation angle, as shown in FIG2 , valve chamber 121 is provided with a first stopper 1211 and a second stopper 1212 along the rotation path of swinging member 123. The first stopper 1211 and the second stopper 1212 are configured to limit the swinging stroke of swinging member 123. When swinging member 123 is located at the first stopper 1211, the first flow path 131 and the second flow path 111 are connected. When swinging member 123 rotates to the first stopper 1211, rotary valve 120 is in the first position. When swinging member 123 is located between the first stopper 1211 and the second stopper 1212 and not in contact with the first stopper 1211, rotary valve 120 is in the second position. When the rotary valve 120 is in the first position, the through hole 12221 connects the first flow path 131 and the second flow path 111 ; when the rotary valve 120 is in the second position, the rotary valve 120 blocks the first flow path 131 and the second flow path 111 .

[0063] Referring to Figures 4 and 5 , to facilitate injection molding of the first and second flow paths 131 and 111, molding holes are provided radially along portions of the flow paths 131 and 111. In this embodiment, the second flow path 111 comprises a horizontal channel 1111 and a vertical channel 1112. The horizontal channel 1111 is located on the same horizontal plane as the first flow path 131, while the vertical channel 1112 is configured to connect the reaction tube 200 and the horizontal channel 1111. To seal the molded flow path, prevent leakage, and facilitate mold manufacturing, a sealing member 400 is provided at the end of the horizontal channel 1111 away from the rotary valve 120.

[0064] Please continue to refer to Figures 4 and 5, and in conjunction with Figure 6, to save materials and reduce costs, the valve stem 122 provided in the embodiment of the present disclosure includes a first valve stem portion 1221, a second valve stem portion 1222, and a connecting portion 1223. One end of the first valve stem portion 1221 is connected to the swing member 123, the other end of the first valve stem portion 1221 is connected to one end of the second valve stem portion 1222, and the connecting portion 1223 is connected to the other end of the second valve stem portion 1222. The first valve stem portion 1221 includes two cross-intersecting plate-like structures, and the second valve stem portion 1222 is provided with a through hole 12221. To prevent liquid leakage, the second valve stem portion 1222 is provided with an annular groove 12222, and an annular sealing gasket is embedded in the annular groove 12222. In order to prevent the first valve stem portion 1221 and the second valve stem portion 1222 from tilting during rotation, the first valve stem portion 1221 in this embodiment is provided with annular protrusions 12211 at intervals along the extension direction of the first valve stem portion 1221. The annular protrusions 12211 are configured to be in close contact with the valve cavity 121 to prevent the first valve stem portion 1221 and the second valve stem portion 1222 from tilting during rotation.

[0065] To facilitate installation of the rotary valve 120 and limit the vertical position of the rotary valve 120, please refer to Figures 4, 5, and 6. The connecting portion 1223 provided in the embodiment of the present disclosure includes a plurality of claws 12231. For each claw 12231, one end of the claw 12231 is connected to the second valve stem portion 1222, and the other end of the claw 12231 is provided with a limiting protrusion 12231. The plurality of claws 12231 are evenly distributed along the circumference of the second valve stem 122, with gaps between adjacent claws 12231. The plurality of claws 12231 are all made of elastic material and can retract inward. A protrusion 1213 is provided within the valve chamber 121. When the limiting protrusion 122311 passes through the position of the protrusion 1213, the multiple claws 12231 retract inward. When the limiting protrusion 122311 passes through the position of the protrusion 1213, the multiple claws 12231 rebound, thereby achieving fixed positioning of the valve stem 122 and the valve chamber 121, thereby enabling installation of the rotary valve 120. The protrusion 1213 on the valve chamber 121 can be used to clamp the connecting portion 1223, ensuring that the through hole 12221 is located on the same horizontal plane as the first flow path 131 and the second flow path 111, facilitating installation. To facilitate installation of the valve body into the valve chamber 121, the end of the claw 12231 away from the second valve stem portion 1222 is a guide arc surface 122312. In this embodiment, there are four claws 12231, which are evenly distributed along the circumference of the second valve stem 122, with a gap between two adjacent claws 12231. Of course, the number of claws 12231 can also be two, three, etc., which is not limited here.

[0066] Referring to Figures 4 and 5 , and in conjunction with Figure 2 , the multiple accommodating chambers 140 provided in the embodiments of the present disclosure include a first wash chamber 141, a second wash chamber 142, a magnetic bead chamber 143, a sample chamber 144, and a magnetic rod sleeve chamber 145. The first wash chamber 141, the second wash chamber 142, the magnetic bead chamber 143, the sample chamber 144, and the magnetic rod sleeve chamber 145 are arranged sequentially from the end of the cartridge body 100 closest to the elution chamber 130 to the end of the cartridge body 100 further from the elution chamber 130. To ensure more accurate test results, one or more wash chambers may be added between the first wash chamber 141 and the second wash chamber 142. The magnetic rod sleeve chamber is configured to hold the magnetic rod sleeve assembly, the sample chamber is configured to hold a sample solvent, the magnetic bead chamber is configured to hold a magnetic bead storage solution containing magnetic beads, the wash chamber is configured to hold a wash solution, and both the first and second elution chambers are configured to hold an eluent.

[0067] According to a sample detection device 1 provided in this embodiment, its working principle is as follows:

[0068] Before testing, the sample solvent is injected into the sample chamber. During testing, the magnetic rod cover grasping mechanism 2300 extracts the magnetic rod cover assembly from the magnetic rod cover chamber and moves it above the magnetic bead chamber. Then, the motion mechanism 2200 drives the magnetic rod cover assembly into the magnetic bead chamber to absorb the magnetic beads. After absorbing the magnetic beads in the magnetic bead chamber, the magnetic rod cover assembly moves from the magnetic bead chamber to the sample chamber, the wash chamber, the second elution chamber, and finally the first elution chamber. Then, the motion mechanism 2200 drives the toggle member 2400 to toggle the swing member 123 of the rotary valve 120, so that the through hole 12221 of the rotary valve 120 connects the first flow path 131 and the second flow path 111. At this time, the movable member drives the piston to move upward relative to the drive chamber 110, and the reaction tube 200 is connected to the drive chamber 110. A negative pressure is formed in the drive chamber 110, so that the liquid in the elution chamber 130 enters the reaction tube 200 through the second flow path 111, the through hole 12221 of the rotary valve 120, and the first flow path 131. Finally, the detection module detects and analyzes the reagent in the reaction tube 200.

[0069] In summary, the reagent cartridge 1000 includes a cartridge body 100, a reaction tube 200, and a driving member 300. The cartridge body 100 includes a driving chamber 110, a rotary valve 120, an elution chamber 130, and a plurality of accommodating chambers 140. The driving chamber 110, the rotary valve 120, the elution chamber 130, and the plurality of accommodating chambers 140 are sequentially connected and integrated into one body. The reaction tube 200 is connected to the driving chamber 110, and the reaction tube 200 is in communication with the driving chamber 110. A first flow path 131 is provided on the side wall of the elution chamber 130, and a second flow path 132 is provided on the side wall of the driving chamber 110. 11; the rotary valve 120 is configured to connect or block the first flow path 131 and the second flow path 111 during rotation; the driving member 300 is movably arranged in the driving chamber 110, and the driving member 300 is configured to form a negative pressure in the driving chamber 110 and the reaction tube 200 during the process of moving relative to the driving chamber 110 when the first flow path 131 and the second flow path 111 are connected, so that the liquid in the elution chamber 130 enters the reaction tube 200 through the second flow path 111, the rotary valve 120, and the first flow path 131 in sequence.

[0070] By using a rotary valve 120 to control the on-off between the first flow path 131 and the second flow path 111, the transfer of liquid between different chambers is achieved, making it possible to conveniently and accurately control the on-off between the first flow path 131 and the second flow path 111. At the same time, a plurality of cavities are provided on the box body 100, and the rotary valve 120 for controlling the connection and blocking of the first flow path 131 in the elution chamber 130 and the second flow path 111 in the drive chamber 110 is set as one of the cavities of the box body 100, thereby simplifying the structure of the reagent cartridge 1000 and reducing production costs. In addition, the driving member 300 and the rotary valve 120 are both located above the box body 100, making it convenient to set a moving mechanism above the reagent cartridge 1000 to drive the driving member 300 and the rotary valve 120 to move, thereby facilitating the integration of the sample detection device 1.

[0071] The above description is only a specific embodiment of the present disclosure, but the protection scope of the present disclosure is not limited thereto. Any changes or substitutions that can be easily conceived by any technician familiar with this technical field within the technical scope disclosed in this disclosure should be covered by the protection scope of the present disclosure. Industrial Applicability

[0072] The reagent cartridge disclosed herein can be used in a sample detection device, and the reagent cartridge uses a rotary valve to control the connection and disconnection between the first flow path and the second flow path, thereby realizing the transfer of liquid between different chambers, so that the connection and disconnection between the first flow path and the second flow path can be conveniently and accurately controlled. At the same time, a plurality of cavities are provided on the cartridge body, and the rotary valve that controls the connection and disconnection between the first flow path in the elution chamber and the second flow path in the drive chamber is set as one of the cavities of the cartridge body, thereby simplifying the structure of the reagent cartridge and reducing production costs. In addition, the driving member and the rotary valve are both located above the cartridge body, making it convenient to set a moving mechanism above the reagent cartridge to drive the driving member and the rotary valve to move, thereby facilitating the integration of the sample detection device.

Claims

1. A reagent cartridge, characterized in that: include: A box body (100), the box body (100) comprising a driving chamber (110), a rotary valve (120), an elution chamber (130) and a plurality of accommodating chambers (140), the driving chamber (110), the rotary valve (120), the elution chamber (130) and the plurality of accommodating chambers (140) being sequentially connected and integrated into one body; A reaction tube (200), the reaction tube (200) being connected to the driving chamber (110), and the reaction tube (200) being in communication with the driving chamber (110); A first flow path (131) is provided on the side wall of the elution chamber (130), and a second flow path (111) is provided on the side wall of the driving chamber (110); The rotary valve (120) is configured to connect or block the first flow path (131) and the second flow path (111) during rotation; A driving member (300), wherein the driving member (300) is movably disposed in the driving chamber (110), and the driving member (300) is configured to form a negative pressure in the driving chamber (110) and the reaction tube (200) during the process of moving relative to the driving chamber (110) when the first flow path (131) and the second flow path (111) are connected, so that the liquid in the elution chamber (130) enters the reaction tube (200) through the second flow path (111), the rotary valve (120), and the first flow path (131) in sequence.

2. The reagent cartridge according to claim 1, characterized in that: The rotary valve (120) comprises a valve cavity (121), a valve stem (122) and a swinging member (123); the upper portion of the valve stem (122) is connected to the swinging member (123); a through hole (12221) is provided on the valve stem (122); the lower portion of the valve stem (122) is located in the valve cavity (121); and the swinging member (123) is configured to rotate the valve stem (122), so that the valve stem (122) can rotate relative to the valve cavity (121); The valve chamber (121) and the cavity openings of the driving chamber (110), the elution chamber (130) and the plurality of accommodating chambers (140) are all located on the upper end surface of the box body (100).

3. The reagent cartridge according to claim 2, characterized in that: The valve cavity (121) is provided with a first limit block (1211) and a second limit block (1212) along the rotation path of the swinging member (123); the first limit block (1211) and the second limit block (1212) are configured to limit the swinging stroke of the swinging member (123).

4. The reagent cartridge according to claim 3, characterized in that: When the swinging member (123) is located at the position of the first limiting block (1211), the first flow path (131) and the second flow path (111) are connected.

5. The reagent cartridge according to claim 3, characterized in that: When the swinging member (123) rotates to the first limit block (1211), the rotary valve (120) is in the first position, and the through hole (12221) connects the first flow path (131) and the second flow path (111); When the swinging member (123) is located between the first limit block (1211) and the second limit block (1212) and is not in contact with the first limit block (1211), the rotary valve (120) is in a second position, and the rotary valve (120) blocks the first flow path (131) and the second flow path (111).

6. The reagent cartridge according to any one of claims 2 to 5, characterized in that: The valve stem (122) comprises a first valve stem portion (1221), a second valve stem portion (1222) and a connecting portion (1223), one end of the first valve stem portion (1221) is connected to the swinging member (123), the other end of the first valve stem portion (1221) is connected to one end of the second valve stem portion (1222), and the connecting portion (1223) is connected to the other end of the second valve stem portion (1222); The first valve stem portion (1221) includes two cross-intersecting plate-shaped structures, and the second valve stem portion (1222) is provided with the through hole (12221).

7. The reagent cartridge according to claim 6, characterized in that: The first valve stem portion (1221) is provided with annular protrusions (12211) at intervals along the extension direction of the first valve stem portion (1221), and the annular protrusions (12211) are configured to be in close contact with the valve cavity (121) to prevent the first valve stem portion (1221) and the second valve stem portion (1222) from tilting during rotation.

8. The reagent cartridge according to claim 6 or 7, characterized in that: The connecting portion (1223) includes a plurality of claws (12231), and for each of the claws (12231), one end of the claw (12231) is connected to the second valve stem portion (1222), and the other end of the claw (12231) is provided with a limiting protrusion (122311); the plurality of claws (12231) are evenly distributed along the circumferential direction of the second valve stem (122), and there is a gap between two adjacent claws (12231); the plurality of claws (12231) are all made of elastic material, and the plurality of claws (12231) are retractable; A protrusion (1213) is provided in the valve cavity (121); when the limiting protrusion (122311) passes through the position of the protrusion (1213), the plurality of claws (12231) retract inwardly; when the limiting protrusion (122311) passes through the position of the protrusion (1213), the plurality of claws (12231) rebound, thereby achieving fixed limiting of the rotary valve (120) and the valve cavity (121).

9. The reagent cartridge according to claim 8, characterized in that: One end of the claw (12231) away from the valve stem portion (1222) is a guiding arc surface (122312).

10. The reagent cartridge according to any one of claims 1 to 9, characterized in that: The second flow path (111) includes a horizontal channel (1111) and a vertical channel (1112), wherein the horizontal channel (1111) and the first flow path (131) are located on the same horizontal plane, and the vertical channel (1112) is configured to connect the reaction tube (200) and the horizontal channel (1111).

11. The reagent cartridge according to any one of claims 1 to 9, characterized in that: The plurality of accommodating chambers (140) are a first cleaning chamber (141), a second cleaning chamber (142), a magnetic bead chamber (143), a sample chamber (144) and a magnetic rod sleeve chamber (145); the first cleaning chamber (141), the second cleaning chamber (142), the magnetic bead chamber (143), the sample chamber (144) and the magnetic rod sleeve chamber (145) are arranged in sequence from one end of the box body (100) close to the elution chamber (130) to one end of the box body (100) away from the elution chamber (130).

12. The reagent cartridge according to any one of claims 1 to 9, characterized in that: Forming holes are provided at radial positions of partial flow sections of the first flow path (131) and the second flow path (111).

13. A sample detection device, characterized in that: It comprises a detection component (2000) and a reagent cartridge (1000) according to any one of claims 1 to 12, wherein the reagent cartridge (1000) is connected to the detection component (2000).

14. The sample detection device according to claim 13, characterized in that: The detection assembly (2000) comprises a detection platform (2100), a motion mechanism (2200), a magnetic rod sleeve grasping mechanism (2300) and a toggle member (2400), and the detection platform (2100) is configured to place the reagent cartridge (1000); The motion mechanism (2200) is connected to the magnetic rod sleeve grasping mechanism (2300) and the toggle member (2400) respectively, the motion mechanism (2200) is located above the detection platform (2100), and the motion mechanism (2200) drives the magnetic rod sleeve grasping mechanism (2300) and the toggle member (2400) to move above the reagent cartridge (1000); The magnetic rod sleeve grabbing mechanism (2300) is configured to grab the magnetic rod sleeve for sample detection; the toggle member (2400) is configured to rotate the rotary valve (120); The motion mechanism (2200) is provided with a groove (2210), and the groove (2210) is configured to clamp the driving member (300). The motion mechanism (2200) moves up and down, so that the driving member (300) moves relative to the driving cavity (110).

15. The sample detection device according to claim 13, characterized in that: The detection assembly (2000) further comprises a movable member, wherein the movable member is configured to drive the driving member (300) to move relative to the driving cavity (110).

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