Cosmetic composition containing extract of centella asiatica with increased natural vitamin content through smart farm technology

Abstract

The present invention relates to a cosmetic composition containing an extract of Centella asiatica with an increased natural vitamin content through smart farm technology and, in particular, to: a method for cultivating Centella asiatica with an increased vitamin C content, the method comprising a step of supplying a nutrient solution composition containing a vitamin-containing plant extract to the roots of Centella asiatica seedlings under white-light irradiation conditions; and a cosmetic composition containing, as an active ingredient, an extract of Centella asiatica with an increased vitamin C content which is obtained by the cultivation method.

Description

Cosmetic composition containing extracts of purslane with increased natural vitamin content using smart farm technology

[0001] The present invention relates to a cosmetic composition containing an extract of purslane with increased natural vitamin content using smart farm technology, and more particularly, to a method for cultivating purslane by supplying a culture solution containing a plant extract containing vitamins under specific light irradiation conditions using smart farm technology, and a technique for using purslane with increased vitamin C content obtained by the cultivation method as a cosmetic.

[0002] Centella asiatica, also known as "tiger grass," is a perennial herb of the Apiaceae family. It grows in temperate and tropical regions such as southern China and Southeast Asia. In Korea, it is distributed in Jeju Island, South Gyeongsang Province, and the southern part of South Jeolla Province. The four main components of Centella asiatica—madecassoside, asiaticoside, madecasic acid, and asiatic acid—are known to promote collagen synthesis at the wound site, thereby inducing wound healing. In addition to skin regeneration, it is also widely used as a treatment for anti-inflammation, skin improvement, immune enhancement, and memory enhancement.

[0003] Vitamin C, also known as ascorbic acid, is an essential nutrient for humans and animals. Because it plays a vital role in antioxidant activity, immune system enhancement, and collagen synthesis, it is widely used as an ingredient in health functional foods, pharmaceuticals, and cosmetics.

[0004] Plants can synthesize vitamin C on their own, and this process can be regulated by external environmental factors. Humans cannot synthesize vitamin C on their own and must obtain it from external sources, making plant-derived vitamin C crucial for human nutrition. Accordingly, various studies have been conducted to efficiently obtain vitamin C from plants.

[0005] In this regard, Korean Patent No. 10-2093373 discloses a method for increasing vitamin C in sprout vegetables of the cabbage family by irradiating them with a single LED light, and Korean Patent No. 10-2566849 discloses a method for cultivating sprout barley with a high saponin content through LED light treatment in a plant factory. However, there is no disclosure regarding the cultivation and use of purslane with increased vitamin C content.

[0006] Based on this background, the present inventors have endeavored to develop a cultivation technique capable of enhancing the vitamin C biosynthesis of Centella asiatica, and as a result, they have confirmed that the level of vitamin C biosynthesis of Centella asiatica is greatly increased when cultivated by supplying a specific culture solution under specific light irradiation conditions using smart farm technology, and that an extract of Centella asiatica with enhanced vitamin C content exhibits excellent antioxidant, whitening, and skin inflammation relief effects, and thus can be usefully used as a cosmetic, thereby completing the present invention.

[0007] One aspect provides a method for cultivating a plant with increased vitamin C content.

[0008] Another aspect is that the method provides a plant with an increased vitamin C content.

[0009] Another aspect provides a method for producing vitamin C derived from Centella asiatica, including a step of extracting Centella asiatica grown by the above method and having an increased content of vitamin C, and a vitamin C derived from Centella asiatica produced by the above method.

[0010] Another aspect provides a cosmetic composition containing an extract of the herb with an increased content of vitamin C as an active ingredient.

[0011] Another aspect provides a composition containing an extract of the purslane with an increased content of vitamin C as an active ingredient for use in anti-oxidation, skin whitening, or skin inflammation relief.

[0012] Another aspect provides a nutrient composition for growing bottle gourd.

[0013] One aspect provides a method for cultivating a purslane with an increased vitamin C content, comprising the step of supplying a nutrient composition containing a plant extract containing vitamins to the roots of purslane seedlings under white light irradiation conditions.

[0014] The plants containing the above vitamins may be fruits or vegetables.

[0015] In one specific example, the plant containing the vitamin may be at least one selected from the group consisting of Hippophae rhamnoides, a tree of the Rosaceae family of the Rosaceae order of the Rosaceae group of the eudicots, roses, linden trees, strawberries, tomatoes, broccoli, cabbage, and citrus fruits, for example, but is not limited thereto.

[0016] The citrus fruit may be, but is not limited to, a lemon.

[0017] The above vitamin may be, for example, but is not limited to, vitamin C.

[0018] The above white light may have a red:green:blue ratio of 5:1 to 4:1 to 4, 5:1 to 3:1 to 3, 5:2 to 4:2 to 4, or 5:2 to 3:2 to 3, for example, 5:3:3.

[0019] The color temperature of the above white light may be 5,400 to 7,000 K, 5,400 to 6,500 K, 5,400 to 6,000 K, 5,700 to 7,000 K, 5,700 to 6,500 K, or 5,700 to 6,000 K.

[0020] The amount of white light above is 500 to 3,500 μmol / m 2 s, 500 to 3,000 μmol / m 2 s, 500 to 2,500 μmol / m 2 s, 1,000 to 3,500 μmol / m 2 s, 1,000 to 3,000 μmol / m 2 s, 1,000 to 2,500 μmol / m 2 s, 1,500 to 3,500 μmol / m 2 s, 1,500 to 3,000 μmol / m 2 s, 1,500 to 2,500 μmol / m 2 s, 2,000 to 3,500 μmol / m 2 s, 2,000 to 3,000 μmol / m 2 s, or 2,000 to 2,500 μmol / m 2 It could be s.

[0021] In one specific example, the white light irradiation conditions are 5,700 to 6,000 K white light at 500 to 2,500 μmol / m 2 It may be investigated with the amount of light of s.

[0022] The electrical conductivity (EC) of the nutrient composition containing the plant extract including the above vitamins may be 0.5 to 2.5, 0.5 to 1.9, 0.5 to 1.5, 1.0 to 2.5, 1.0 to 1.9, or 1.0 to 1.5, for example, 0.5 to 1.9.

[0023] The pH of the nutrient composition containing the plant extract including the above vitamins may be 5.0 to 6.0, 5.0 to 5.8, 5.2 to 6.0, 5.2 to 5.8, 5.4 to 6.0, 5.4 to 5.8, 5.6 to 6.0, or 5.6 to 5.8.

[0024] In one specific example, the nutrient composition containing the plant extract including the vitamin may satisfy the conditions of EC (Electrical Conductivity) 0.5 to 1.9 and pH 5.6 to 5.8.

[0025] The nutrient composition may comprise 0.01 to 5 wt%, 0.01 to 4 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1 wt%, 0.1 to 5 wt%, 0.1 to 4 wt%, 0.1 to 3 wt%, 0.1 to 2 wt%, 0.1 to 1 wt%, 0.5 to 5 wt%, 0.5 to 4 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, or 0.5 to 1 wt%, for example, 1 wt%, of a plant extract containing vitamins.

[0026] The cultivation method of the above-mentioned plant may be hydroponics or soil cultivation.

[0027] Another aspect is to provide a plant grown by the above cultivation method with increased vitamin C content.

[0028] Another aspect provides a method for producing vitamin C derived from purslane, including a step of extracting purslane grown by the above cultivation method and having an increased vitamin C content, and a vitamin C derived from purslane produced by the above production method.

[0029] Another aspect provides a cosmetic composition containing, as an active ingredient, an extract of a purslane grown by the above cultivation method and having an increased vitamin C content.

[0030] The above cosmetic composition may additionally contain vitamin C.

[0031] The above cosmetic composition may be used for antioxidant, skin whitening or skin inflammation improvement.

[0032] Another aspect provides a composition containing an extract of a plant with increased vitamin C content cultivated by the above cultivation method as an active ingredient for use in anti-oxidation, skin whitening, or skin inflammation relief.

[0033] Another aspect provides a nutrient solution composition for growing purslane, comprising a plant extract containing vitamins; at least one trace element selected from the group consisting of nitrogen, phosphorus, potassium, lime, boron, iron, zinc, molybdenum, kaolin, manganese and copper; and at least one nutrient selected from the group consisting of glutamic acid, citric acid and amino acid.

[0034] The plants containing the above vitamins may be fruits or vegetables.

[0035] In one specific example, the plant containing the vitamin may be at least one selected from the group consisting of sea buckthorn (Hippophae rhamnoides), roses, linden trees, strawberries, tomatoes, broccoli, cabbage, and citrus fruits, for example, but not limited to sea buckthorn.

[0036] The citrus fruit may be, but is not limited to, a lemon.

[0037] The above vitamin may be, for example, but is not limited to, vitamin C.

[0038] The electrical conductivity (EC) of the nutrient composition may be 0.5 to 2.5, 0.5 to 1.9, 0.5 to 1.5, 1.0 to 2.5, 1.0 to 1.9, or 1.0 to 1.5, for example, 0.5 to 1.9.

[0039] The pH of the nutrient composition may be 5.0 to 6.0, 5.0 to 5.8, 5.2 to 6.0, 5.2 to 5.8, 5.4 to 6.0, 5.4 to 5.8, 5.6 to 6.0, or 5.6 to 5.8.

[0040] In one specific example, the nutrient composition may satisfy the conditions of EC (Electrical Conductivity) 0.5 to 1.9 and pH 5.6 to 5.8.

[0041] The nutrient composition may comprise 0.01 to 5 wt%, 0.01 to 4 wt%, 0.01 to 3 wt%, 0.01 to 2 wt%, 0.01 to 1 wt%, 0.1 to 5 wt%, 0.1 to 4 wt%, 0.1 to 3 wt%, 0.1 to 2 wt%, 0.1 to 1 wt%, 0.5 to 5 wt%, 0.5 to 4 wt%, 0.5 to 3 wt%, 0.5 to 2 wt%, or 0.5 to 1 wt%, for example, 1 wt%, of a plant extract containing vitamins.

[0042] The method of cultivating Centella asiatica according to the daily aspect promotes the biosynthesis of vitamin C in Centella asiatica without affecting the growth of Centella asiatica at all, and thus can be usefully used for natural vitamin C production. In addition, the extract of Centella asiatica with increased vitamin C content cultivated by the above method exhibits excellent antioxidant, whitening, and anti-inflammatory activities, and thus can be usefully used as a cosmetic for skin improvement.

[0043] Figure 1 is a graph showing the results of analyzing the vitamin C content in bottle gourd harvested after cultivation under different light irradiation conditions using high performance liquid chromatography technology.

[0044] FIG. 2 is a graph showing the results of analyzing the vitamin C content in bottle gourd harvested after cultivation by supplying a nutrient solution containing a pine tree extract according to one embodiment using high performance liquid chromatography technology.

[0045] Figure 3 is a graph showing the results of a cytotoxicity evaluation of a bottle gourd extract manufactured according to one embodiment.

[0046] Figure 4 is a graph showing the results of an anti-inflammatory efficacy evaluation of a bottle gourd extract manufactured according to one embodiment.

[0047] Figure 5 is a graph showing the results of an antioxidant efficacy evaluation of a bottle gourd extract manufactured according to one embodiment.

[0048] Figure 6 is a graph showing the results of an evaluation of the whitening efficacy of a bottle gourd extract manufactured according to one embodiment.

[0049] Hereinafter, the present invention will be described in more detail.

[0050] When the plant is grown by supplying a culture solution containing plant extracts containing vitamins under specific light irradiation conditions according to a specific cultivation method, the vitamin C content is increased, and the extract of this plant has excellent skin improvement activity.

[0051] Native to North Asia and Europe, the sea buckthorn (Hippophae rhamnoides), also known as the vitamin tree, has been widely used as a natural remedy in countries such as China and Russia to treat ulcers, wounds, skin diseases, and burns. It is also known as the Alpine sandthorn due to its cold-hardiness and long thorns. Sea buckthorn fruit contains over 100 rich bioactive compounds, including vitamins, flavonoids, and amino acids.

[0052] According to one aspect, a method for cultivating a purslane with an increased vitamin C content is provided, which comprises the step of supplying a nutrient composition containing a plant extract containing vitamins to the roots of purslane seedlings under white light irradiation conditions.

[0053] In one specific example, the white light irradiation conditions are 5,700 to 6,000 K white light at 500 to 2,500 μmol / m 2 With a light intensity of s, more specifically 1,500 to 2,500 μmol / m 2 It may be investigated with the amount of light of s.

[0054] In one embodiment, white light with a red:green:blue ratio of 5:3:3 is applied at a concentration of 500 to 2,500 μmol / m during the cultivation of the plant. 2 The purslane was grown under irradiation with s light intensity and a 16-h / 8-h light / dark cycle. The purslane grown under white light irradiation under the above conditions showed a higher vitamin C content than the purslane grown under red, blue, and green light irradiation, respectively (Fig. 1).

[0055] In one specific example, the nutrient composition used for growing bottle gourd may include a plant extract containing vitamins.

[0056] In one specific example, the nutrient solution composition used for growing bottle gourd may have an EC (Electrical Conductivity) of 0.5 to 1.9 and a pH of 5.6 to 5.8.

[0057] According to one specific example, the nutrient solution composition is made by mixing a plant extract containing vitamins into a basic culture solution containing nutrients such as nitrogen, phosphorus, potassium, which are essential components for plant growth, and trace elements such as lime, boron, iron, zinc, molybdenum, manganese, and copper, and glutamic acid, citric acid, and amino acids.

[0058] In one specific example, the plant extract containing the vitamin may be included in an amount of 0.01 to 1 wt% relative to the total weight of the nutrient composition.

[0059] In one specific example, the plant extract containing the vitamin may be prepared by extracting the raw material plant at a low temperature, for example, at 4 to 10°C, using water as a solvent.

[0060] In this case, purslane can be cultivated using hydroponic or soil-based methods. Specifically, purslane can be cultivated using smart farm technology. A smart farm refers to a farm that integrates agricultural technology with information and communication technology (ICT) to remotely control the crop growth environment appropriately through IT devices such as smartphones and PCs in orchards and greenhouses, and maintains the optimal environment based on big data.

[0061] By applying smart farm technology and irradiating white light, the nutrient solution can be supplied directly to the roots of the bottle gourd seedlings through the nutrient solution supply system of the hydroponic cultivation machine or by irrigating the roots of the bottle gourd seedlings through soil cultivation.

[0062] In one embodiment, under the white light irradiation conditions, it was confirmed that when a culture solution containing a pine tree extract mixed with the basic culture solution was supplied to the roots of the bottle gourd seedlings and grown, the vitamin C content of the bottle gourd increased significantly (Fig. 2).

[0063] By extracting the purslane grown by the above method and having an increased vitamin C content, natural vitamin C derived from purslane can be efficiently produced. At this time, solvents such as water, acetic acid, ethanol, and methanol can be used as extraction solvents, but are not limited thereto.

[0064] The centella asiatica grown by the above method has an increased content of vitamin C, a physiologically active substance, and exhibits superior skin improvement activity compared to general centella asiatica extracts. Specifically, the centella asiatica extract exhibited excellent antioxidant activity (Test Example 5), skin whitening activity (Test Example 6), and anti-inflammatory activity (Test Example 4). In addition, it was confirmed that the centella asiatica extract with an increased vitamin C content exhibited an even superior antioxidant effect when used together with ascorbic acid (vitamin C) (Test Example 5, Fig. 5).

[0065] The above-mentioned extract of the herb as an effective ingredient may be contained in a proportion of 0.001 to 30 wt% based on the total weight of the composition.

[0066] The cosmetic composition according to the aspect can be manufactured in any formulation that is conventionally manufactured, and examples thereof include toner, essence, cream, cleansing foam, cleansing water, cleansing oil, pack, body lotion, body oil, gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, lip balm, etc.

[0067]

[0068] [Example]

[0069] The present invention is described in more detail below through the following examples and test examples. However, the following examples are merely illustrative of the present invention, and the scope of the present invention is not limited to these examples.

[0070]

[0071] Example 1. Preparation of nutrient solution composition

[0072] A nutrient solution composition for use in growing bottle gourd was prepared as follows.

[0073]

[0074] Basic nutrient solution composition

[0075] A culture medium containing 5.5% nitrogen, 2.2% phosphorus, 5.3% potassium, 2% lime, 0.05% boron, 0.03% iron, 0.0001% zinc, 0.0002% molybdenum, 1% kaolin, 0.007% manganese, 0.0007% copper, and glutamic acid, citric acid, and amino acids by weight was prepared.

[0076]

[0077] Preparation of plant extracts containing vitamins (sea buckthorn extract)

[0078] The vitamin C content of sea buckthorn berries, roses, lemons, tomatoes, strawberries, broccoli, and cabbage was measured, and the results are shown in Table 1. As a result, an extract was prepared using sea buckthorn berries, which were found to contain the most vitamin C.

[0079] Raw vitamin C content (per 100g) Sea buckthorn fruit 530 mg, rose 426 mg, lemon 53 mg, tomato 20 mg, strawberry 58.8 mg, broccoli 98 mg, cabbage 36.6 mg

[0080]

[0081] Specifically, 10 g of the fruit of the sea buckthorn tree was crushed, mixed with 200 g of purified water, and extracted at 4°C for 24 hours. After filtration under reduced pressure, the extract was distilled using a rotary evaporator.

[0082]

[0083] Preparation of nutrient solution composition

[0084] A nutrient composition for use in cultivating bottle gourd was prepared by mixing 1 wt% of a pine tree extract into the above basic nutrient composition.

[0085]

[0086] Test Example 1: Analysis of Vitamin C Content in Centella asiatica according to Light Irradiation Conditions

[0087] By investigating various LED light sources and light intensities, we cultivated the following plants.

[0088] White light (R:G:B=5:3:3), blue light (470 nm), red light (660 nm), and green light (540 nm) light sources were used at 500, 1,000, 2,000, and 2,500 μmol / m 2 The bottle gourd was grown for two weeks in a conventional manner under light conditions of s. During cultivation, the basic nutrient solution of Example 1 was diluted in water at a volume ratio of 1 / 1,000 and supplied to the roots of the bottle gourd seedlings. The plants were grown under a light irradiation of 16 hours / 8 hours light / dark cycle and harvested.

[0089] The vitamin C content of the harvested dandelion was analyzed using liquid chromatography (High Performance Liquid Chromatography), and the results are shown in Figure 1.

[0090] As shown in Figure 1, white light (R:G:B=5:3:3) is 2,500 μmol / m 2 When treated with light intensity of s, the total amount of vitamin C was approximately 8.6 times higher than that of other light sources, and it was confirmed that it increased approximately 2.2 times compared to low light intensity from the same light source.

[0091]

[0092] Comparative Example 1, Example 2: Cultivation of bottle gourd according to nutrient solution treatment

[0093] White light (R:G:B=5:3:3), which showed high effectiveness in the above test example, 2,500 μmol / m 2 Under the light conditions of s, the basic nutrient solution of Example 1 (Comparative Example 1) and the nutrient solution containing the extract of the sea buckthorn tree (Example 2) were diluted in water at a volume ratio of 1 / 1,000 and supplied to the roots of the seedlings of the bottle gourd for 2 weeks, and the seedlings were cultivated and harvested under a light irradiation cycle of 16 hours / 8 hours.

[0094]

[0095] Test Example 2: Analysis of Vitamin C Content in Centella asiatica according to Nutrient Solution Treatment

[0096] The vitamin C content of the above-mentioned cultivated plant was analyzed using liquid chromatography (High Performance Liquid Chromatography), and the results are shown in Figure 2.

[0097] As shown in Figure 2, it was confirmed that the content of vitamin C was approximately twice as high when growing by supplying a nutrient solution mixed with a pine tree extract compared to when growing by supplying a basic nutrient solution.

[0098]

[0099] Comparative Example 2, Example 3: Preparation of Centella asiatica extract

[0100] An extract was prepared using the plant grown under the cultivation conditions that showed the highest vitamin C content.

[0101] Normal conditions (white light, 500 μmol / m 2 The plant (comparative example 2) harvested by cultivating it under the above conditions (white light, 2,500 μmol / m 2 s, a nutrient solution containing extracts of the Chinese juniper tree was used to harvest the plant (Example 3), and 10 g of each was mixed with 200 g of 70% ethanol, extracted at room temperature for 3 hours, filtered, and the liquid extract was concentrated under reduced pressure to obtain an extract.

[0102]

[0103] Test Example 3: Cytotoxicity Evaluation (MTT Assay)

[0104] To confirm the cytotoxicity of the above-mentioned extract of the plant (Comparative Example 2, Example 3), an MTT assay was performed. Human dermal fibroblast (HDFa) cell line was seeded in a 96-well plate at a density of 1x10 5After inoculating at a concentration of 10 cells / mL, the cells were cultured under 5% carbon dioxide (C02) at 37°C for 18 hours.

[0105] After culturing, the existing medium was removed, washed with PBS buffer, and then the new medium was filled with the extract of Centella asiatica at various concentrations and cultured under the same conditions for another 24 hours. To measure cell viability, 5 mg / mL of MTT solution was added, and the formazan formed over 4 hours was dissolved in DMSO and the absorbance at 570 nm was measured using an ELISA reader, which is shown in Figure 3.

[0106] As shown in Fig. 3, when the extract of the plant with increased vitamin C content was treated at various concentrations, no cytotoxicity was observed in any concentration range.

[0107]

[0108] Test Example 4: Anti-inflammatory efficacy evaluation

[0109] To confirm the anti-inflammatory efficacy of the above-mentioned extract of Centella asiatica (Comparative Example 2, Example 3), the gene expression of IL-1β and IL-6 was analyzed. Human keratinocyte cell line (HaCaT) was seeded in a 6-well plate at a density of 1x10 5 After inoculating at a concentration of 10 cells / mL, the cells were cultured at 37°C for 18 hours under 5% carbon dioxide (C02). 10 μg / ml of Poly I:C, an immunostimulant, and 10 ng / ml of IL-4 protein were added to the culture medium, and dexamethasone, which has an anti-inflammatory effect, was used at a concentration of 1 μM as a positive control. The above Centella asiatica extract was administered at various concentrations and cultured under the same conditions for 4 hours.

[0110] After culture, cells were harvested, washed with phosphate buffered saline (PBS), and placed in PureLink ® Total RNA was extracted using RNA Mini Kit (Thermo Fisher Scientific).

[0111] Changes in the expression of inflammation-related genes IL-1β and IL-6 were measured using quantitative real-time PCR (qRT-PCR), which conjugates fluorescent substances to DNA products amplified in polymerase chain reaction (PCR) and continuously detects the fluorescent substances. The fluorescence intensities of the IL-1β and IL-6 genes were standardized to actin values, converted to 100% of the control group, and the differences were compared to measure the expression levels of each gene, and the results are shown in Figure 4.

[0112] As shown in Fig. 4, when the extract of the purslane with increased vitamin C content was treated at different concentrations, it was confirmed that the effect of suppressing inflammation factors was higher than when the extract of the purslane grown under normal conditions was treated.

[0113]

[0114] Test Example 5: Antioxidant Efficacy Evaluation

[0115] To confirm the antioxidant efficacy of the above Centella asiatica extract (Comparative Example 2, Example 3), a DPPH radical scavenging activity test was conducted. The DPPH radical scavenging activity was measured by measuring the reducing power of the sample for DPPH radicals. DPPH (1,1-Diphenyl-2-picrylhydrazyl) reagent was dissolved in methanol to prepare a 0.1 mM solution, and Centella asiatica extracts prepared at various concentrations and ascorbic acid as a positive control group were added to 0.99 ml of the DPPH solution in a 96-well plate, mixed, and reacted at 37°C for 30 minutes. The absorbance was measured at 517 nm using an ELISA reader, and the DPPH radical scavenging activity (%) of the sample and positive control group was calculated using the following formula and is shown in Fig. 5.

[0116]

[0117] DPPH radical scavenging activity (%) = {1-(absorbance of positive control or sample / absorbance of control)} × 100

[0118]

[0119] As shown in Fig. 5, it was confirmed that the antioxidant activity was enhanced when the extract of the purslane of Example 3 was treated compared to the extract of the purslane of Comparative Example 2. In addition, when the extract of the purslane with increased ascorbic acid and vitamin C content (Example 3) was mixed and treated, even superior antioxidant activity was exhibited.

[0120]

[0121] Test Example 6: Whitening Efficacy Evaluation

[0122] To confirm the whitening effect of the above-mentioned extract of Centella asiatica (Comparative Example 2, Example 3), the amount of melanin production was measured and analyzed. Murine melanoma (B16-F10) cells were seeded in a 6-well plate at a density of 1 × 10 per well in a medium containing an appropriate amount of FBS (fetal bovine serum). 5 The cells were inoculated with a dog and cultured at 5% CO2, 37℃ until they attached. After replacing the medium with the sample diluted to an appropriate concentration, the cells were cultured at 5% CO2, 37℃ for 72 hours. The cells were washed with PBS and treated with trpysin-EDTA to harvest them. The supernatant was removed to obtain a pellet, and the pellet was dried at 80℃ for 1 hour. Then, 300 ㎕ of 1 N sodium hydroxide solution containing 10% DMSO was added and reacted in a constant temperature bath at 80℃ to obtain intracellular melanin. The absorbance was measured at 490 nm using a microplate reader and is shown in Figure 6.

[0123] As shown in Fig. 6, when the extract of the purslane of Example 3 with increased vitamin C content was treated, it was confirmed that melanin production was suppressed more than when the extract of the purslane generally grown was treated.

Claims

1. Under white light irradiation conditions, A method for cultivating purslane with increased vitamin C content, comprising the step of supplying a nutrient composition containing a plant extract containing vitamins to the roots of purslane seedlings.

2. A method for cultivating centella asiatica with increased vitamin C content, wherein the plant containing the vitamin in claim 1 is at least one selected from the group consisting of Hippophae rhamnoides, roses, linden trees, strawberries, tomatoes, broccoli, cabbage, and citrus fruits.

3. In claim 1, the white light irradiation conditions are 5,700 to 6,000 K white light at 500 to 2,500 μmol / m 2 A method for cultivating a plant with increased vitamin C content by irradiating it with light of s.

4. A method for cultivating a purslane with increased vitamin C content, wherein the nutrient solution composition containing a plant extract containing vitamins in claim 1 satisfies the conditions of EC (Electrical Conductivity) 0.5 to 1.9 and pH 5.6 to 5.

8.

5. In claim 1, the method for cultivating the purslane is a method for cultivating purslane with increased vitamin C content, wherein the method is hydroponic cultivation or soil cultivation.

6. A bottle gourd with increased vitamin C content cultivated by the method of any one of claims 1 to 5.

7. A method for producing vitamin C derived from purslane, comprising a step of extracting purslane grown by the method of any one of claims 1 to 5 and having an increased vitamin C content.

8. Vitamin C derived from the plant produced by the method of claim 7.

9. A cosmetic composition containing, as an active ingredient, an extract of a purslane plant having an increased vitamin C content cultivated by the method of any one of claims 1 to 5.

10. A cosmetic composition according to claim 9, wherein the cosmetic composition additionally contains vitamin C.

11. A cosmetic composition according to claim 9, wherein the cosmetic composition is for antioxidant use, skin whitening use, or skin inflammation improvement use.

12. A nutrient solution composition for growing purslane, comprising a plant extract containing vitamins; at least one trace element selected from the group consisting of nitrogen, phosphorus, potassium, lime, boron, iron, zinc, molybdenum, kaolin, manganese, and copper; and at least one nutrient selected from the group consisting of glutamic acid, citric acid, and amino acid.

13. A method for cultivating centella asiatica with increased vitamin C content, wherein the plant containing the vitamin according to claim 12 is at least one selected from the group consisting of Hippophae rhamnoides, roses, linden trees, strawberries, tomatoes, broccoli, cabbage, and citrus fruits.

14. A nutrient solution composition for cultivating purslane, wherein the nutrient solution composition satisfies the conditions of EC (Electrical Conductivity) 0.5 to 1.9 and pH 5.6 to 5.8 according to claim 12.

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