Cosmetic composition comprising double fermentation product of kunzea ericoides extract

Abstract

Provided is a composition comprising a double fermentation product of Kunzea ericoides obtained by primarily fermenting a Kunzea ericoides extract with Lactobacillus sp. microorganisms and secondarily fermenting the Kunzea ericoides extract with Bacillus sp. microorganisms. The composition comprises the double fermentation product, thereby having excellent effects such as antioxidation, anti-inflammation, skin moisturization, exfoliation, and skin microorganism control, and thus can be effectively used as a raw material for cosmetics and the like.

Description

Cosmetic composition comprising a double fermentation product of white tea tree extract

[0001] The present invention relates to a cosmetic composition comprising a double fermented product of white tea tree extract.

[0002] Recently, as interest in cosmetic raw materials that are harmless and safe to the skin and have skin-improving functionality has increased, research is ongoing to develop natural extracts as cosmetic raw materials.

[0003] White tea tree (Kunzea ericoides), also known as kanuka, was first discovered in New Zealand. White tea tree leaf extract is used in cosmetics as an antioxidant and flavoring agent, while white tea tree leaf oil is used as a flavoring agent. Accordingly, some research is being conducted on the use of white tea tree extract as a cosmetic ingredient.

[0004] However, to date, there has been no report on the skin improvement effect of white tea tree fermentation.

[0005] One aspect is to provide a composition comprising a fermented product of white tea tree extract.

[0006] Specifically, the above aspect provides a cosmetic composition, a composition for external use in the skin, or a pharmaceutical composition comprising a double fermented white tea tree extract obtained by first fermenting the white tea tree extract with a microorganism of the genus Lactobacillus and then secondarily fermenting the white tea tree extract with a microorganism of the genus Bacillus.

[0007] Another aspect provides a method for producing a fermented product of white tea tree extract.

[0008] Another aspect provides a use of a double fermented white tea tree for preparing a composition for antioxidant, anti-inflammatory, skin soothing, skin irritation relief, skin moisturizing, skin barrier strengthening, exfoliating, or skin microbiome regulation.

[0009] One aspect provides a composition comprising a fermented product of white tea tree extract.

[0010] Specifically, the composition comprises a white tea tree double fermentation product obtained by first fermenting a white tea tree (Kunzea ericoides) extract with a Lactobacillus sp. microorganism and then second fermenting it with a Bacillus sp. microorganism.

[0011] The above white tea tree (Kunzea ericoides) can be used in the form of whole plants, leaves, stems, roots, seeds, etc., and is not limited to a specific part. In one specific example, the white tea tree can be a leaf of the white tea tree.

[0012] The term "extract" includes all substances obtained by extracting a component of a natural product, regardless of the extraction method, extraction solvent, extraction conditions, extracted component, or form of the extract, and also includes substances that can be obtained by processing or treating the component of a natural product by another method after extraction. For example, the processing or treatment may include dilution, concentration, drying, purification, fractionation, filtration, etc. Accordingly, the extract may include an extract, a diluted or concentrated extract, a dried product obtained by drying the extract, or a adjusted or purified product thereof, or a fraction obtained by fractionating the extract.

[0013] The above extraction method may use known natural product extraction methods such as hot water extraction, ethanol extraction, heating extraction, cold extraction, reflux extraction, reflux cooling extraction, ultrasonic extraction, and subcritical extraction, but is not limited thereto.

[0014] The above extraction solvent may be selected from water, an organic solvent, or a mixture thereof. The water may be distilled water or purified water. The organic solvent may include, but is not limited to, one or more of C1 to C6 alcohols, acetone, ether, ethyl acetate, diethyl ether, ethyl methyl ketone, and chloroform.

[0015] In one embodiment, the extraction solvent of the extract may be water, a C1 to C6 alcohol, or a mixture thereof. In another embodiment, the extraction solvent of the extract may be water, a C1 to C4 alcohol, or a mixture thereof. In another embodiment, the extraction solvent of the extract may be a mixture of water and a C1 to C4 alcohol. In another embodiment, the extraction solvent of the extract may be butylene glycol. In another embodiment, the extraction solvent of the extract may be 10% (v / v) to 90% (v / v), 30% (v / v) to 90% (v / v), 50% (v / v) to 90% (v / v), 60% (v / v) to 80% (v / v), or 70% (v / v) butylene glycol.

[0016] The above extraction conditions refer to conditions for extracting components of natural products, such as extraction time and extraction temperature.

[0017] The above extraction time may be 30 minutes to 168 hours, 30 minutes to 120 hours, 30 minutes to 100 hours, 30 minutes to 80 hours, 30 minutes to 60 hours, 30 minutes to 40 hours, 1 hour to 168 hours, 1 hour to 120 hours, 1 hour to 100 hours, 1 hour to 80 hours, 1 hour to 60 hours, 1 hour to 40 hours, 10 hours to 168 hours, 10 hours to 120 hours, 10 hours to 100 hours, 10 hours to 80 hours, 10 hours to 60 hours, 10 hours to 40 hours, 10 hours to 30 hours, 24 hours to 168 hours, 24 hours to 120 hours, 24 hours to 100 hours, or 24 hours to 80 hours, It can be appropriately selected depending on other conditions such as extraction solvent and extraction temperature.

[0018] The above extraction temperature may be 1 to 100°C, 10 to 100°C, 50 to 100°C, 70 to 100°C, 80 to 100°C, 1 to 60°C, 2 to 60°C, 2 to 50°C, or 2 to 40°C, but may be appropriately selected depending on other conditions such as the extraction solvent and extraction time. In one specific example, the extraction temperature may be about 90°C.

[0019] The above white tea tree double fermentation product can be obtained by the steps of: preparing a white tea tree extract; fermenting the white tea tree extract primarily by a Lactobacillus microorganism to prepare a white tea tree primary fermentation product; and fermenting the white tea tree primary fermentation product primarily by a Bacillus microorganism to prepare a white tea tree double fermentation product.

[0020] Any microorganism of the genus Lactobacillus (Lactobacillus sp.) known in the art may be used. The above Lactobacillus microorganism is not limited to a specific type, but may be selected from the group consisting of, for example, L. acidophilus, L. casei, L. gasseri, L. delbrueckii ssp. bulgaricus, L. helveticus, L. fermentum, L. paracasei, L. plantarum, L. reuteri, L. rhamnosus, and L. salivarius. In one specific example, the Lactobacillus genus microorganism may be Lactobacillus plantarum.

[0021] The above Bacillus sp. microorganism includes all aerobic or facultative anaerobic bacilli widely distributed in nature. The Bacillus sp. microorganism is not limited to a specific type, but may be selected from the group consisting of, for example, Bacillus subtilis, Bacillus coagulans, B. velezensis, B. licheniformis, B. polyfermenticus, B. methylotrophicus, and B. amyloliquefaciens. In one specific example, the Bacillus sp. microorganism may be Bacillus subtilis. The above Bacillus genus microorganism may be a strain that produces keratinase.

[0022] The above Lactobacillus genus microorganism or Bacillus genus microorganism can be used in the form of a microbial culture solution. A culture medium and culture conditions for culturing the Lactobacillus genus microorganism or Bacillus genus microorganism can be appropriately selected or modified by a person skilled in the art.

[0023] The above fermentation conditions may be selected at an appropriate temperature and time depending on the type of microorganism. For example, the fermentation may be performed at 20 to 40°C, but is not particularly limited thereto. In addition, the fermentation may be performed for 1 to 168 hours, 1 to 120 hours, 1 to 72 hours, 1 to 48 hours, 12 to 168 hours, 12 to 120 hours, 12 to 72 hours, 12 to 48 hours, 24 to 168 hours, 24 to 120 hours, 24 to 72 hours, 24 to 48 hours, 48 ​​to 168 hours, 48 ​​to 120 hours, or 48 to 72 hours, but is not particularly limited thereto.

[0024] The above fermentation medium can be appropriately selected depending on the type of microorganism. Depending on the type of microorganism, the medium can be a known medium or a conventional medium capable of culturing the microorganism.

[0025] The above fermented product may include a fermented liquid obtained by fermenting white tea tree by microorganisms, a diluted or concentrated liquid of the fermented liquid, a dried product obtained by drying the fermented liquid, or a controlled or purified product thereof, a fraction obtained by fractionating the same, etc.

[0026] The fermented product may be a supernatant or filtrate of a fermented liquid obtained by fermenting white tea tree with microorganisms. The supernatant or filtrate may be obtained from the fermented liquid from which the fungi have been removed. Accordingly, the fermented product may be a supernatant of the fermented liquid. Alternatively, the fermented product may be a fermented filtrate.

[0027] The fermented product may be included in an amount of 0.0001 to 99.9999 wt%, 0.0001 to 90 wt%, 0.0001 to 50 wt%, 0.0001 to 10 wt%, 0.001 to 99.9999 wt%, 0.001 to 90 wt%, 0.001 to 50 wt%, 0.001 to 10 wt%, 0.01 to 99.9999 wt%, 0.01 to 90 wt%, 0.01 to 50 wt%, 0.01 to 20 wt%, or 0.01 to 10 wt% based on the total weight of the composition, but a person skilled in the art may select an appropriate content in consideration of the desired effect and formulation, etc.

[0028] The above composition may contain a double fermented white tea tree as an active ingredient.

[0029] The above “included as an active ingredient” means that the ingredient is added to an extent that it can exhibit the effect mentioned in this specification, and includes formulating in various forms by adding various ingredients as auxiliary ingredients for drug delivery and stabilization.

[0030] The above composition may be a composition for improving skin condition, improving skin beauty, improving skin health, or preventing or treating skin diseases.

[0031] The above white tea tree double fermentation product may have ABTS radical scavenging activity. Accordingly, a composition containing the above white tea tree double fermentation product may have an antioxidant effect.

[0032] The above white tea tree double fermentation product may have NO (Nitric Oxide) inhibitory properties. Accordingly, a composition containing the above white tea tree double fermentation product may have anti-inflammatory, skin soothing, and skin irritation relief effects.

[0033] The above white tea tree double fermentation product may have the ability to increase AQP-3 (Aquaporin-3) expression. Accordingly, a composition containing the above white tea tree double fermentation product may have skin moisturizing and skin barrier strengthening effects.

[0034] The above-mentioned white tea tree double fermentation product may have proteolytic enzyme activity. Accordingly, a composition containing the above-mentioned white tea tree double fermentation product may have an exfoliating effect.

[0035] The above-mentioned white tea tree double-fermented product may have a skin microbiome control effect. Regulating the skin microbiome may mean reducing harmful skin bacteria without affecting beneficial skin bacteria. The beneficial skin bacteria may be, for example, Staphylococcus epidermidis. The harmful skin bacteria may be, for example, Pseudomonas aeruginosa, Staphylococcus aureus, etc.

[0036] The ABTS radical scavenging activity, NO inhibition activity, AQP-3 expression increasing activity, protein decomposition enzyme activity, and skin microorganism control effects of the above white tea tree double fermentation product may be significantly superior to those of unfermented white tea tree extract or lactobacillus fermentation filtrate.

[0037] Therefore, the composition containing the above white tea tree double fermentation product can be used for antioxidant, anti-inflammatory, skin soothing, skin irritation relief, skin moisturizing, skin barrier strengthening, exfoliation, or skin microbiome control.

[0038] The term "skin moisturizing" can refer to any action that maintains skin moisture or prevents moisture loss. Skin moisturizing can help improve wrinkles and increase skin elasticity.

[0039] The term "skin barrier strengthening" can refer to any action that enhances the function of the skin barrier, which is located on the outermost layer of the skin and prevents moisture and nutrient loss.

[0040] The above “anti-inflammatory” may be used interchangeably with “improving inflammation” and “inflammation suppression” and may include any action that suppresses the production of nitric oxide (NO), for example.

[0041] The above “skin soothing” may include all actions that calm and stabilize symptoms of damaged or irritated skin (e.g., fever, pain, redness, itchiness, etc.) or restore the skin.

[0042] The above “skin disease” may be a disease caused by damage to the skin barrier function or a skin inflammatory disease.

[0043] The above-mentioned skin barrier function impairment can refer to any change that appears on the skin due to a decline or damage to the skin barrier function. Examples include increased wrinkling, dryness, dermatitis, atopic dermatitis, allergic dermatitis, and acne.

[0044] The above skin inflammatory disease may be any one selected from the group consisting of skin wounds, dermatitis, atopic dermatitis, pruritus, eczematous skin disease, dry eczema, erythema, urticaria, psoriasis, rash, and acne.

[0045] The term "skin health" can mean all actions that maintain the function of the skin and protect the skin from external stimuli, and can include, for example, maintaining the skin barrier function; moisturizing the skin; protecting it from external factors such as ultraviolet rays; maintaining elasticity; and soothing and repairing skin damage.

[0046] The term "prevention" encompasses suppressing the onset of a disease. The term "treatment" encompasses suppressing, alleviating, or eliminating the development of a disease. The term "improvement" can refer to any action that at least reduces the severity of symptoms, such as parameters associated with alleviating or curing a condition.

[0047] In one specific example, the composition may be a cosmetic composition.

[0048] In addition to the effective ingredients disclosed herein, the above cosmetic composition may additionally include components commonly used in cosmetic compositions, functional additives, etc., and may include conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, surfactants, dispersants, thickeners, preservatives, vitamins, pigments, fragrances, etc., and cosmetically acceptable carriers.

[0049] The above cosmetic composition may be manufactured in any conventionally manufactured formulation. For example, the cosmetic composition may have a cosmetic formulation of a toner, cream, essence, cleansing foam, cleansing water, pack, ampoule, body lotion, body oil, body gel, shampoo, rinse, hair conditioner, hair gel, foundation, lipstick, mascara, or makeup base.

[0050] Alternatively, the composition may be a composition for external use in the skin.

[0051] The above-mentioned external preparation for skin may be a cream, gel, ointment, skin emulsifier, skin suspension, transdermal patch, drug-containing bandage, lotion, or a combination thereof. The above-mentioned external preparation for skin may be appropriately mixed with ingredients commonly used in external preparations for skin such as cosmetics or medicines, such as aqueous ingredients, oily ingredients, powder ingredients, alcohols, moisturizers, thickeners, UV absorbers, whitening agents, preservatives, antioxidants, surfactants, fragrances, colorants, various skin nutrients, or a combination thereof, as needed.

[0052] Alternatively, the composition may be a pharmaceutical composition.

[0053] The pharmaceutical composition may additionally comprise a pharmaceutically acceptable diluent or carrier. The diluent may be lactose, corn starch, soybean oil, microcrystalline cellulose, mannitol, or a combination thereof. The carrier may be an excipient, a disintegrant, a binder, a glidant, or a combination thereof. The excipient may be microcrystalline cellulose, lactose, low-substituted hydroxycellulose, or a combination thereof. The disintegrant may be calcium carboxymethylcellulose, sodium starch glycolate, calcium dihydrogen phosphate anhydrous, or a combination thereof. The binder may be polyvinylpyrrolidone, low-substituted hydroxypropyl cellulose, hydroxypropyl cellulose, or a combination thereof. The glidant may be magnesium stearate, silicon dioxide, talc, or a combination thereof.

[0054] The pharmaceutical composition may be formulated as an oral or parenteral dosage form. Oral dosage forms may include granules, powders, liquids, tablets, capsules, dry syrups, etc. Parenteral dosage forms may include injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, etc.

[0055] Another aspect provides a method for producing a fermented product of white tea tree extract.

[0056] Specifically, the above method may be a method for producing a double fermented white tea tree product.

[0057] The method comprises the steps of preparing a white tea tree extract; the step of first fermenting the white tea tree extract with a Lactobacillus genus microorganism to prepare a white tea tree primary fermentation product; and the step of second fermenting the white tea tree primary fermentation product with a Bacillus genus microorganism to prepare a white tea tree double fermentation product.

[0058] Another aspect provides the use of a double fermented white tea tree for preparing a composition for antioxidant, anti-inflammatory, skin soothing, skin irritation relief, skin moisturizing, skin barrier strengthening, exfoliating, or skin microbiome regulation.

[0059] The above composition may be a cosmetic composition, a skin external preparation composition, or a pharmaceutical composition.

[0060] Details regarding the above white tea tree double fermentation product are as described above. Accordingly, the above white tea tree double fermentation product may be obtained by first fermenting a white tea tree extract with a Lactobacillus sp. microorganism and then second fermenting it with a Bacillus sp. microorganism.

[0061] Duplicate contents are omitted in consideration of the complexity of this specification, and terms not otherwise defined in this specification have meanings commonly used in the technical field to which the present invention belongs.

[0062] The composition according to the aspect includes a white tea tree double fermented product obtained by double-fermenting white tea tree extract with microorganisms of the genus Lactobacillus and microorganisms of the genus Bacillus, and thus has excellent effects such as antioxidant, anti-inflammatory, skin moisturizing, exfoliation, and skin microorganism control, and thus can be usefully used as a raw material for cosmetics, etc.

[0063] Figure 1 is a graph showing ABTS radical scavenging activity (%). L-Ascorbic acid: positive control, white tea tree extract: white tea tree extract of Preparation Example 1, Lactobacillus plantarum: lactobacillus fermentation filtrate of Preparation Example 3, White-Keratinase: white tea tree double fermentation of Example 1.

[0064] Figure 2 is a graph confirming NO inhibition ability. L-NMMA: positive control, white tea tree extract: white tea tree extract of manufacturing example 1, Lactobacillus plantarum: lactobacillus fermentation filtrate of manufacturing example 3, White-Keratinase: white tea tree double fermentation of example 1.

[0065] Figure 3 is a graph showing the level of AQP-3 gene expression. White tea tree extract: White tea tree extract of Preparation Example 1, Lactobacillus plantarum: Lactobacillus fermentation filtrate of Preparation Example 3, White-Keratinase: White tea tree double fermentation of Example 1, CaCl2: Positive control.

[0066] Figure 4 shows the results of a paper disk experiment confirming protein decomposition efficacy. After fermentation: White tea tree double fermentation product of Example 1, before fermentation: White tea tree extract of Preparation Example 1.

[0067] Figure 5 is a graph showing changes in the number of skin microorganisms before and after co-cultivation in the double fermentation of white tea tree of Example 1.

[0068] The present invention will be described in more detail below through examples. However, these examples are intended to exemplify the present invention and the scope of the present invention is not limited to these examples.

[0069] [Manufacturing example]

[0070] Manufacturing Example 1. Manufacturing of white tea tree extract

[0071] 100 g of white tea tree (Kunzea ericoides) leaves were extracted with 500 mL of 70% (v / v) butylene glycol at 90°C for 24 hours. Afterwards, the original material was removed using an 8 μm filter, and the extract was filtered using a 0.45 μm membrane filter to prepare a white tea tree extract.

[0072] Manufacturing Example 2. Preparation of Lactobacillus culture solution

[0073] 1,000 mL of purified water was added to 55 g of MRS medium, autoclaved at 121°C for 15 minutes, and cooled to room temperature. Afterwards, Lactobacillus plantarum strain (accession number: KCTC14687BP) isolated from Centella asiatica was inoculated to 2% of the culture volume, and cultured for 48 hours at 180 rpm and 37°C.

[0074] Manufacturing Example 3. Manufacturing of Lactobacillus Ferment Filtrate

[0075] The lactobacillus culture solution of the above manufacturing example 2 was centrifuged at 4,500 rpm for 20 minutes to remove the bacterial cells, and the supernatant was obtained, which was then filtered through a 0.45 ㎛ membrane filter to prepare a lactobacillus fermentation filtrate.

[0076] Manufacturing Example 4. Preparation of Bacillus culture solution

[0077] 1,000 mL of purified water was added to 55 g of nutrient medium, autoclaved at 121°C for 15 minutes, and cooled to room temperature. Afterwards, Bacillus subtilis isolated from natto was inoculated to 2% of the medium volume, and cultured for 48 hours at 180 rpm and 37°C.

[0078] [Example]

[0079] Example 1. Preparation of white tea tree fermentation product

[0080] Example 1-1. Preparation of primary fermented white tea tree

[0081] The white tea tree extract of Manufacturing Example 1 was fermented with the lactobacillus culture solution of Manufacturing Example 2 to produce a white tea tree fermented product.

[0082] Specifically, the Lactobacillus culture of Manufacturing Example 2 was inoculated into the white tea tree extract of Manufacturing Example 1 at a concentration of 2% (v / v) of the extract volume, and cultured at 37°C and 150 rpm for 72 hours to produce a white tea tree primary fermentation product. The fermentation product was centrifuged at 4,500 rpm for 20 minutes to remove bacterial cells, and the supernatant was then filtered through a 0.45 ㎛ membrane filter to obtain a filtrate. The filtrate was used as the white tea tree primary fermentation product in the following experiment.

[0083] Example 1-2. Preparation of secondary fermentation product of white tea tree

[0084] The white tea tree primary fermentation product of Example 1-1 was fermented with the Bacillus culture solution of Manufacturing Example 4 to produce a white tea tree secondary fermentation product.

[0085] Specifically, the Bacillus culture of Preparation Example 4 was inoculated into the white tea tree primary fermentation of Example 1-1 at a concentration of 2% (v / v) of the extract volume, and cultured at 37°C and 150 rpm for 72 hours to produce a white tea tree secondary fermentation. The fermentation was centrifuged at 4,500 rpm for 20 minutes to remove bacterial cells, and the supernatant was filtered through a 0.45 ㎛ membrane filter to obtain a filtrate. The filtrate was used as a white tea tree double fermentation in the following experiment. The white tea tree double fermentation was named "White-Keratinase."

[0086] [Experimental Example]

[0087] Experimental Example 1. Measurement of antioxidant activity

[0088] An experiment was conducted to confirm the antioxidant activity of a double fermented white tea tree product according to one embodiment.

[0089] Specifically, the degree of reduction of ABTS radical by reaction with a sample was measured using ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), a stable free radical. The white tea tree extract of Preparation Example 1, the lactobacillus fermentation filtrate of Preparation Example 3, or the white tea tree double fermentation of Example 1 were used as samples, and each sample was used at a concentration of 1% (v / v). A group treated with 100 ㎍ / ㎖ of L-ascorbic acid was used as a positive control group.

[0090] Figure 1 is a graph showing ABTS radical scavenging activity (%).

[0091] As a result, as shown in Fig. 1, it was confirmed that the white tea tree extract of Preparation Example 1, the lactobacillus fermentation filtrate of Preparation Example 3, and the white tea tree double fermentation product of Example 1 all had antioxidant activity. In particular, it was confirmed that the white tea tree double fermentation product of Example 1 had significantly superior antioxidant activity compared to the white tea tree extract or the lactobacillus fermentation filtrate.

[0092] Therefore, it was found that the double fermented white tea tree of Example 1 had an excellent antioxidant effect.

[0093] Experimental Example 2. Confirmation of anti-inflammatory and skin-soothing effects.

[0094] An experiment was conducted to confirm the anti-inflammatory and skin soothing effects of a double-fermented white tea tree extract according to one embodiment.

[0095] Specifically, after treating mouse macrophage Raw 264.7 cells with LPS (liposaccharides), each sample was treated at a concentration of 1% (v / v) to measure the level of NO (Nitric Oxide) gene expression. The group treated with nothing (MO: Media Only) was used as the negative control group. The group treated with 30 ㎍ / ㎖ of L-NMMA was used as the positive control group.

[0096] Figure 2 is a graph confirming NO inhibition ability.

[0097] As a result, as shown in Fig. 2, it was confirmed that the white tea tree extract of Manufacturing Example 1 and the white tea tree double fermentation product of Example 1 significantly inhibited the expression of NO genes that cause skin irritation. In particular, the white tea tree double fermentation product of Example 1 exhibited the best NO inhibition ability.

[0098] Therefore, it was found that the double fermented white tea tree of Example 1 had excellent anti-inflammatory, skin soothing, and skin irritation relief effects.

[0099] Experimental Example 3. Confirmation of skin moisturizing and skin barrier strengthening effects.

[0100] An experiment was conducted to confirm the skin moisturizing and skin barrier strengthening effects of a double fermented white tea tree product according to one embodiment.

[0101] Specifically, after culturing human keratinocytes, each sample was treated at a concentration of 1% (v / v) to determine the level of AQP-3 (Aquaporin-3) gene expression. The group treated with 2 mM CaCl served as a positive control group.

[0102] Figure 3 is a graph showing the level of AQP-3 gene expression.

[0103] As a result, as shown in Fig. 3, it was confirmed that the white tea tree double fermentation product of Example 1 had a significantly superior AQP-3 expression increasing effect compared to the white tea tree extract of Preparation Example 1 or the lactobacillus fermentation filtrate of Preparation Example 3.

[0104] Therefore, it was found that the double fermented white tea tree of Example 1 had excellent skin moisturizing and skin barrier strengthening effects.

[0105] Experimental Example 4. Confirmation of the exfoliating effect

[0106] The exfoliation effect according to the protein-decomposing enzyme activity of the double fermented white tea tree according to one embodiment was confirmed.

[0107] Specifically, a circular paper disc was placed on a 1% skim milk agar plate and cultured, and the protein decomposition efficacy was confirmed through the diameter of the clear zone.

[0108] Figure 4 shows the results of a paper disk experiment that confirmed protein decomposition efficacy.

[0109] As a result, as shown in Fig. 4, the white tea tree double fermentation of Example 1 formed a clear zone with a clearer and larger diameter than the white tea tree extract of Preparation Example 1, confirming that the protein decomposition efficacy according to the colony was superior.

[0110] Therefore, it was found that the double fermentation of white tea tree of Example 1 had excellent protein decomposition efficacy and thus excellent exfoliation effect.

[0111] Experimental Example 5. Confirmation of the skin microbiome regulation effect.

[0112] An experiment was conducted to confirm the skin microbiome control effect of a double-fermented white tea tree extract according to one embodiment.

[0113] Specifically, TSB (Tryptic Soy Broth, BD Difco, USA) was used as the microbial medium. The growth of the bacteria was performed by inoculating 1 × 10 of the corresponding strain into the appropriate medium. 6After inoculating at a concentration of CFU / mL and culturing at 37°C for 24 h, the growth rate was compared by measuring the absorbance at 600 nm, or by confirming the actual number of viable cells through the serial dilution method. The growth rate was compared as a percentage based on the experimental group to which the same amount of phosphate buffered saline (PBS) as the test sample was added. 1 mL of 10% TSB was added to a 12-well plate for co-culture of harmful and beneficial skin bacteria, and harmful bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) were added at a density of 1 × 10 6 CFU / mL concentration. A 0.4 ㎛ cell culture insert was placed on top, and 1 × 10 of Staphylococcus epidermidis was added to 1 mL of 10% TSB. 6 Inoculated at a concentration of 10 CFU / mL. As a control, the same amount of phosphate-buffered saline (PBS) was used. After 12 h of incubation at 32°C, the number of each strain was checked and the degree of growth was compared.

[0114] Figure 5 is a graph showing changes in the number of skin microorganisms before and after co-cultivation in the double fermentation of white tea tree of Example 1.

[0115] As a result, as shown in Fig. 5, it was confirmed that there was no change in the number of S. epidermidis, a beneficial skin bacterium, and S. aureus and P. aeruginosa, harmful skin bacteria, before co-culture. However, after co-culture in the double-fermented white tea tree of Example 1, it was confirmed that there was no effect on S. epidermidis, a beneficial skin bacterium, but the number of S. aureus and P. aeruginosa, harmful skin bacteria, decreased.

[0116] Therefore, it was found that the double-fermented white tea tree of Example 1 had a skin microbiome control effect by reducing skin harmful bacteria without affecting skin beneficial bacteria.

[0117] In summary, it was confirmed that the white tea tree double fermentation product obtained by first fermenting white tea tree extract with lactobacillus and then second fermenting it with bacillus had the following effects:

[0118] (i) It has excellent antioxidant effect because it has ABTS radical scavenging ability;

[0119] (ii) It has NO inhibitory properties, so it has excellent anti-inflammatory, skin soothing, and skin irritation relief effects;

[0120] (iii) It has the ability to increase AQP-3 expression, so it has excellent skin moisturizing and skin barrier strengthening effects;

[0121] (iv) It has excellent exfoliation effect because it has protein-decomposing enzyme activity; and

[0122] (v) It has an excellent effect in regulating skin microorganisms because it reduces harmful skin bacteria (e.g., S. aureus, P. aeruginosa) without affecting beneficial skin bacteria (e.g., S. epidermidis).

Claims

1. A cosmetic composition comprising a double fermented white tea tree extract obtained by first fermenting a white tea tree (Kunzea ericoides) extract with a Lactobacillus sp. microorganism and then second fermenting it with a Bacillus sp. microorganism.

2. A cosmetic composition according to claim 1, wherein the extraction solvent of the extract is water, a C1 to C6 alcohol, or a mixture thereof.

3. A cosmetic composition according to claim 1, wherein the Lactobacillus microorganism is selected from the group consisting of L. acidophilus, L. casei, L. gasseri, L. delbrueckiissp.bulgaricus, L. helveticus, L. fermentum, L. paracasei, L. plantarum, L. reuteri, L. rhamnosus, and L. salivarius.

4. A cosmetic composition according to claim 1, wherein the Bacillus microorganism is selected from the group consisting of B. subtilis, B. coagulans, B. velezensis, B. licheniformis, B. polyfermenticus, B. methylotrophicus, and B. amyloliquefaciens.

5. A cosmetic composition according to claim 1, having at least one effect selected from ABTS radical scavenging ability, NO (Nitric Oxide) inhibition ability, and AQP-3 (Aquaporin-3) expression increasing ability.

6. A cosmetic composition having protein-decomposing enzyme activity according to claim 1.

7. A cosmetic composition having a skin microbiome control effect that reduces skin harmful bacteria without affecting skin beneficial bacteria according to claim 1.

8. A cosmetic composition according to claim 7, wherein the skin beneficial bacteria is Staphylococcus epidermidis, and the skin harmful bacteria is Pseudomonas aeruginosa or Staphylococcus aureus.

9. A cosmetic composition according to claim 1, which is used for antioxidant, anti-inflammatory, skin soothing, skin irritation relief, skin moisturizing, skin barrier strengthening, exfoliation, or skin microbiome control.

10. Use of a double fermented white tea tree for manufacturing a composition for antioxidant, anti-inflammatory, skin soothing, skin irritation relief, skin moisturizing, skin barrier strengthening, exfoliation, or skin microbiome control. The above white tea tree double fermentation product is obtained by first fermenting a white tea tree (Kunzea ericoides) extract with a Lactobacillus sp. microorganism and then second fermenting it with a Bacillus sp. microorganism.

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