Composition for improving skin condition

Abstract

The present invention relates to a composition for improving skin condition, comprising a mixed extract of Actinidia argute and Perilla frutescens as an active ingredient. The composition of the present invention has the effect of strengthening the skin barrier and inhibiting the formation of skin wrinkles, and thus can be effectively used as a composition for improving skin condition.

Description

Composition for improving skin condition

[0001] This patent application claims priority to Republic of Korea Patent Application No. 10-2023-0129833, filed with the Korean Intellectual Property Office on September 26, 2023, the disclosure of which is incorporated herein by reference.

[0002] The present invention relates to a composition for improving skin condition, comprising a mixed extract of perilla and perilla leaves as an effective ingredient.

[0003]

[0004] The skin, the outermost organ of the human body, serves as a vital barrier, protecting the body from external aggressors and retaining moisture. The stratum corneum, the outermost layer of the skin, resists physical and chemical damage and prevents the infiltration of microorganisms and harmful chemicals. Furthermore, keratinocytes, Langerhans cells, fibroblasts, mast cells, dendritic cells, and capillary endothelial cells form a complex immune system that responds to external antigens to protect the body. Skin undergoes constant change due to external stimuli such as ultraviolet rays, chemicals, heat, physical friction, and fine dust, the most prominent of which is skin aging.

[0005] Skin aging is caused by a decline in the regenerative capacity of skin cells. Skin aging can be divided into intrinsic aging, which occurs as we age, and extrinsic aging, which is caused by external factors and environmental influences. The primary cause of extrinsic aging is ultraviolet (UV) rays, and skin aging caused by UV rays is also called "photoaging." Sunlight contains visible light, infrared rays, and ultraviolet (UV) rays. UV rays have a wavelength of 200 to 400 nm and are known to cause various skin damage with continuous exposure. UV rays are classified into UVA (Ultraviolet-A), UVB (Ultraviolet-B), and UVC (Ultraviolet-C) depending on the wavelength, with UVA and UVB primarily affecting the human body. UVA rays have a wavelength of 320 nm to 400 nm, reaching deep into the skin due to their long wavelength, causing systemic immune suppression and contributing to the development of skin cancer. While they do not cause immediate damage, they penetrate over time, leading to wrinkles, loss of skin elasticity, and skin cancer. UVB rays have a wavelength of 280 nm to 320 nm, which is shorter than UVA and directly damages DNA, increasing the risk of cancer. They also cause immediate inflammation, sunburn, and skin cancer. The most prominent symptoms of photoaging include fine wrinkles, spots, and pigmentation, which are proportional to the duration and intensity of sun exposure.

[0006] Accordingly, active research is being conducted on pharmaceuticals, cosmetics, and foods that can suppress UV-induced skin damage, strengthen the skin barrier, inhibit photoaging, and suppress wrinkle formation. However, these products often cause skin irritation or have minimal effects. Therefore, there is a pressing need to develop compositions that can effectively suppress UV-induced skin damage without causing skin irritation.

[0007] Numerous papers and patents are referenced and cited throughout this specification. The disclosures of these cited papers and patents are incorporated herein by reference in their entirety to provide a clearer understanding of the state of the art and the scope of the invention.

[0008]

[0009] The present inventors have diligently researched and developed a composition that effectively suppresses UV-induced skin damage without causing skin irritation. As a result, they discovered that a complex herbal extract of perilla and perilla leaves increases the expression of filaggrin in the epidermis, ceramides in the epidermis, and hyaluronic acid in the dermis, thereby suppressing UV-induced skin moisture loss, strengthening the skin barrier, inhibiting wrinkle formation, and suppressing photoaging, thereby completing the present invention.

[0010] Accordingly, the purpose of the present invention is to provide a food composition for improving skin condition, which contains a mixed extract of persimmon and perilla leaves as an effective ingredient.

[0011] Another object of the present invention is to provide a health functional food containing the above food composition as an active ingredient.

[0012] Another object of the present invention is to provide a cosmetic composition for improving skin condition, which comprises a mixed extract of persimmon and perilla leaves as an effective ingredient.

[0013] Another object of the present invention is to provide a pharmaceutical composition for preventing or treating skin damage caused by ultraviolet rays, which comprises a mixed extract of perilla and perilla leaves as an active ingredient.

[0014] Other objects and advantages of the present invention will become more apparent from the detailed description, claims and drawings below.

[0015]

[0016] According to one aspect of the present invention, the present invention provides a food composition for improving skin condition comprising a mixed extract of perilla and perilla leaves as an effective ingredient.

[0017] The inventors of the present invention have diligently researched and developed a composition that effectively suppresses UV-induced skin damage without causing skin irritation. As a result, they discovered that a complex herbal extract of perilla and perilla leaves increases the expression of epidermal filaggrin, epidermal ceramide, and dermal hyaluronic acid, thereby suppressing UV-induced skin moisture loss, strengthening the skin barrier, inhibiting wrinkle formation, and suppressing photoaging.

[0018] In the present specification, the term "Actinidia" includes Actinidia arguta, Actinidia kolomikta, Actinidia polygama, combinations thereof, or closely related plants thereof. The term "Actinidia" includes at least one selected from the group consisting of the fruit, stem, vine, leaf, and root of the Actinidia tree.

[0019] In this specification, Perillae Folium refers to the leaves and tips of Perilla frutescens Britton var. acuta Kudo or Perilla frutescens Britton var. crispa Decaisne of the Labiatae family, which are 30 to 60 cm tall and are called red pepper, chajogi, and wrinkled chajogi. It is produced in Korea, China, etc., and is used for food or medicine in Korea. Perillae Folium is purple and has a unique flavor, the stem is straight with a angular shape, and the leaves have a round or wedge-shaped base and serrated edges. The flowers are light purple, bloom in racemes around August to September, and the shapes of the leaves and stems are similar to those of perilla. Its main components are known to be perilla aldehyde, d-limonene, α-pinene, cyanin, sterin, menthol, rosmarinic acid, and luteolin, and it is known to prevent food poisoning and have strong antibacterial properties when consumed with meat or fish.

[0020] The extracts of the perilla and perilla leaves used in the present invention can be purchased or obtained by direct extraction from the herbal medicine. The extraction can be performed after cutting or crushing each herbal medicine into an appropriate size.

[0021] Meanwhile, the mixed extract of persimmon and perilla leaves (composite herbal extract) used in the present invention can be prepared by mixing individual extracts of persimmon and perilla leaves, or by treating a mixture of persimmon and perilla leaves with an extraction solvent.

[0022] In one embodiment of the present invention, the mixed extract (composite herbal extract) of perilla and perilla leaves used in the present invention may be prepared by mixing perilla and perilla leaves in a weight ratio of 1-10:1-10, 1:1-10, or 1-10:1, 1-9:1-9, 1:1-9, or 1-9:1, 1-8:1-8, 1:1-8, or 1-8:1, 1-7:1-7, 1:1-7, or 1-7:1, 1-5:1-5, 1:1-5, or 1-5:1, 1-4:1-4, 1:1-4, or 1-4:1, 1-3:1-3, 1:1-3, or 1-3:1, and extracting with an extraction solvent. More specifically, the mixed extract of the above-mentioned persimmon and perilla leaves (composite herbal extract) can be prepared by mixing persimmon and perilla leaves in a weight ratio of 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1, respectively, and extracting with an extraction solvent, but is not limited thereto.

[0023] In one embodiment of the present invention, the mixed extract (composite herbal extract) of perilla and perilla leaves may be prepared by mixing the individual extracts of perilla and perilla leaves in a weight ratio of 1-10:1-10, 1:1-10, or 1-10:1, 1-9:1-9, 1:1-9, or 1-9:1, 1-8:1-8, 1:1-8, or 1-8:1, 1-7:1-7, 1:1-7, or 1-7:1, 1-5:1-5, 1:1-5, or 1-5:1, 1-4:1-4, 1:1-4, or 1-4:1, 1-3:1-3, 1:1-3, or 1-3:1. More specifically, the mixed extract of the above-mentioned persimmon and perilla leaves (composite herbal extract) can be prepared by mixing the individual extracts of the persimmon and perilla leaves in a weight ratio of 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, or 1:1, but is not limited thereto.

[0024] When the extract used in the composition of the present invention is obtained by directly extracting it from the raw materials of persimmon and perilla leaves, various extraction solvents such as polar solvents or nonpolar solvents can be used.

[0025] Suitable polar solvents include (i) water, (ii) lower alcohols having C1 to C6 (specifically, methanol, ethanol, propanol, butanol, normal-propanol, iso-propanol, normal-butanol, 1-pentanol, 2-butoxyethanol or ethylene glycol), (iii) acetic acid, (iv) dimethyl-formamide (DMFO) and (v) dimethylsulfoxide (DMSO). Suitable non-polar solvents include acetone, acetonitrile, ethyl acetate, methyl acetate, fluoroalkanes, pentane, hexane, 2,2,4-trimethylpentane, decane, cyclohexane, cyclopentane, diisobutylene, 1-pentene, 1-chlorobutane, 1-chloropentane, o-xylene, diisopropyl ether, 2-chloropropane, toluene, 1-chloropropane, chlorobenzene, benzene, diethyl ether, diethyl sulfide, chloroform, dichloromethane, 1,2-dichloroethane, aniline, diethylamine, ether, carbon tetrachloride and THF (Tetrahydrofuran).

[0026] The amount of the above extraction solvent may vary depending on the amount of the persimmon and perilla leaves to be extracted, and specifically, it may be a volume (ml) of 1 to 20 times the weight (g) of the persimmon; the weight of the perilla leaves; or the weight (g) of the mixture of the persimmon and perilla leaves, specifically, a volume (ml) of 5 to 15 times the volume (ml), more specifically, a volume (ml) of 5 to 12 times the volume (ml) or 7 to 12 times the volume (ml), and most specifically, a volume (ml) of 8 times the weight of the mixture of the persimmon and perilla leaves, but is not limited thereto.

[0027] The extraction temperature of the extract of the present invention is not particularly limited, and may be, for example, 0°C to 120°C, and specifically, 15°C to 100°C, 20°C to 95°C, 25°C to 95°C, 30°C to 95°C, 35°C to 95°C, 40°C to 95°C, 50°C to 95°C, 60°C to 95°C, 70°C to 95°C, 80°C to 95°C, or a temperature within the range of 85°C to 95°C, and more specifically, may be 10°C, 20°C, 30°C, 40°C, 50°C, 60°C, 70°C, 80°C, or 90°C, but is not limited thereto. In a specific embodiment of the present invention, the extraction temperature of the extract of the present invention is 85°C to 95°C.

[0028] The extraction time of the extract of the present invention is not particularly limited, and may be, for example, 1 hour to 10 days, specifically, 1 hour to 72 hours, 1 hour to 48 hours, 1 hour to 36 hours, 1 hour to 24 hours, 1 hour to 12 hours, 1 hour to 10 hours, 1 hour to 6 hours, 1 hour to 5 hours, or 1 hour to 4 hours, and more specifically, 2 hours to 72 hours, 2 hours to 48 hours, 2 hours to 36 hours, 2 hours to 24 hours, 2 hours to 12 hours, 2 hours to 10 hours, 2 hours to 6 hours, 2 hours to 5 hours, 2 hours to 4 hours, 3 hours to 72 hours, 3 hours to 48 hours, 3 hours to 36 hours, 3 hours to 24 hours, 3 hours to 12 hours, 3 hours to It can be 10 hours, 3 to 6 hours, or 3 to 5 hours, most specifically, but not limited to, 3 hours.

[0029] The extract of the present invention can be extracted using known natural product extraction methods. For example, it can be extracted using cold extraction, hot water extraction, ultrasonic extraction, reflux cooling extraction, or heating extraction. Specifically, it can be extracted using hot water extraction or reflux cooling extraction, and the extraction can be repeated from 1 to 10 times.

[0030] According to one embodiment of the present invention, the extracts of the perilla and perilla leaves used in the present invention can be extracted using an organic solvent, water, or a mixed solvent thereof. The organic solvents include lower alcohols having a carbon content of 1 to 6 carbons, petroleum ether, hexane, benzene, chloroform, methylene chloride, ether, ethyl acetate, and acetone.

[0031] In addition, according to another embodiment of the present invention, the extract of the persimmon and perilla leaves of the present invention can be prepared by extracting with the above-described water, C1 to C6 lower alcohol or a mixed solvent thereof, or by extracting and concentrating (under reduced pressure) and then further extracting or fractionating with an organic solvent selected from the group consisting of the above-described petroleum ether, hexane, benzene, chloroform, methylene chloride, ether, ethyl acetate, and acetone.

[0032] In the present invention, the extracts of persimmon and perilla leaves can be used in the form of a crude extract extracted by a solvent, and can also be used after being purified to a high purity.

[0033] The term 'extract' used herein has the meaning commonly used in the art as a crude extract as described above, but in a broad sense, it also includes fractions obtained by further fractionation of the extract. That is, the extracts of perilla and perilla leaves include not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, fractions obtained by passing the extract through an ultrafiltration membrane having a certain molecular weight cut-off value, separation by various chromatographies (designed for separation according to size, charge, hydrophobicity, or affinity), and other fractions obtained through various additional purification methods are also included in the extracts of perilla and perilla leaves of the present invention. The extracts of perilla and perilla leaves used in the present invention can be manufactured in a powdered state by additional processes such as concentration under reduced pressure, distillation under reduced pressure, and freeze-drying or spray drying.

[0034] The mixing ratio between the herbal ingredients described in this specification is calculated based on the weight of the solid content from which the solvent has been removed (in the case of a mixture of herbal extracts) or the weight of the herbal medicine itself (in the case of an extract of a mixed herbal medicine).

[0035] In this specification, the term “comprising an effective ingredient” means containing an effective amount capable of exhibiting a skin condition improvement effect, for example, an effect of inhibiting photoaging, inhibiting wrinkle formation, inhibiting skin damage caused by ultraviolet rays, moisturizing the skin, improving skin elasticity, and strengthening the skin barrier.

[0036] As used herein, the term 'between' or '-' written between two numbers means an interval between the numbers written before and after the numbers.

[0037] In one embodiment of the present invention, the improvement in skin condition described above may be at least one selected from the group consisting of inhibition of photoaging, inhibition of skin wrinkle formation, inhibition of skin damage caused by ultraviolet rays, skin moisturizing, improvement of skin elasticity, and strengthening of the skin barrier.

[0038] Exposure of the skin to UV rays triggers physical and biochemical changes within the skin tissue, resulting in epithelial cell proliferation, hyperpigmentation, DNA damage through excessive formation of reactive oxygen species, and abnormal activation of various enzymes and proteins. These changes impair the normal skin barrier function, which regulates absorption of external substances and moisture evaporation, and increase epidermal thickness. Furthermore, they damage the structure of the dermis, contributing to the formation of wrinkles.

[0039] In one embodiment of the present invention, the mixed extract (complex herbal extract) of the present invention can increase skin moisture retention and reduce transepidermal water loss (TEWL). Transepidermal water loss (TEWL) refers to the amount of moisture radiated from the skin. A higher TEWL indicates a decline in the skin's moisturizing function, and at the same time, indicates that the skin's inherent barrier function has been damaged.

[0040] The mixed extract (complex herbal extract) of the present invention can increase skin moisture retention and reduce transepidermal water loss (TEWL), thereby preventing UV-induced skin moisture loss and skin barrier damage and helping skin barrier function function normally. Therefore, the composition of the present invention can be usefully used as a skin moisturizing or skin barrier strengthening composition.

[0041] In one embodiment of the present invention, the mixed extract (complex herbal extract) of the present invention increases the epidermal ceramide content.

[0042] The epidermis is composed of several layers, and cells that proliferate in the stratum corneum, the lowest layer, migrate upwards, undergoing a differentiation process and ultimately reaching the stratum corneum. Ceramides are molecules created by the combination of sphingoids and fatty acids, and the polarity of ceramides formed by these structural factors plays an important role in maintaining the layered structure of the sebum film and maintaining the skin barrier function. When ceramide production is reduced due to exogenous factors such as ultraviolet rays, temperature, and humidity, or endogenous factors such as genetics, aging, stress, and nutritional status, the layered structure of the sebum film is destroyed, trans-epidermal water loss (TEWL) increases, and skin moisture retention decreases. As a result, epidermal hyperproliferation, increased itching, and inflammation are also induced secondarily.

[0043] As described above, the mixed extract (complex herbal extract) of the present invention increases the epidermal ceramide content, thereby preventing damage to the skin barrier and enabling the skin barrier function to function normally. Therefore, the composition of the present invention can be usefully used as a composition for strengthening the skin barrier.

[0044] In one embodiment of the present invention, the mixed extract (composite herbal extract) of the present invention not only increases the expression of filaggrin, but also promotes the production of epidermal amino acids from filaggrin, thereby strengthening the skin barrier and suppressing the formation of skin wrinkles.

[0045] Filaggrin is one of several structural proteins expressed by keratinocytes during the differentiation stage. It is involved in differentiation from the basal layer of the epidermis to the stratum corneum, and is also a major component of the natural moisture factor (NMF) essential for maintaining moisture in skin tissue, so it is used as a major indicator of skin moisture retention and skin barrier function.

[0046] Free amino acids in the epidermis are generated during the process of profilaggrin being converted to filaggrin and then broken down. Profilaggrin is a high molecular weight protein (>300 kDa) with glutamate, serine, proline, glycine, and histidine accounting for more than 80% of its total amino acid composition, and is produced in the stratum granulosum, the middle layer of the epidermis. The produced profilaggrin moves to the upper layer of epidermal cells and is broken down into filaggrin. Filaggrin is broken down into free amino acids, and various proteolytic enzymes, such as PPARγ, protein phosphatase type 2A, peptidylarginine deiminase (PADI3), caspase-14 (CASP14), transglutaminases (TGM), kallikrein-5, calpain, and furin, are reported to be involved in this process.

[0047] The mixed extract (complex herbal extract) of the present invention not only increases the expression of filaggrin, but also increases the expression of PADI3 (peptidylarginine deiminase 3) and CASP14 (caspase-14), which are involved in the filaggrin degradation process, thereby promoting the production of epidermal amino acids from filaggrin, thereby strengthening the skin barrier and suppressing the formation of wrinkles. Therefore, the composition of the present invention can be usefully used as a composition for strengthening the skin barrier or a composition for improving skin wrinkles.

[0048] In one embodiment of the present invention, the mixed extract (complex herbal extract) of the present invention can increase the expression of dermal hyaluronic acid.

[0049] Hyaluronic acid (HA) is a type of glycosaminoglycan (GAG) that is a major extracellular matrix of the dermis, along with collagen and elastin. Hyaluronic acid is produced by hyaluronic acid synthase (HAS) in dermal fibroblasts and epidermal keratinocytes, and functions as an extracellular matrix of the dermis or is distributed in the intercellular spaces of the epidermis. The content of hyaluronic acid decreases with intrinsic aging and photoaging caused by ultraviolet rays, and a decrease in the content of hyaluronic acid causes the formation of skin wrinkles, loss of skin elasticity, and loss of skin moisture retention. Among the three types of hyaluronic acid synthase (HAS1, HAS2, and HAS3), HAS2 and HAS3 are mainly involved in the production of hyaluronic acid in dermal fibroblasts and epidermal keratinocytes, respectively. On the other hand, four types of hyaluronic acid decomposition enzymes (Hyaluronidase; HYAL) (HYAL1, HYAL2, HYAL3, HYAL4) are known, of which HYAL1 and HYAL2 are mainly involved in the decomposition of hyaluronic acid in the dermis and epidermis, and in particular, HYAL1 is the main decomposition enzyme of dermal and epidermal hyaluronic acid.

[0050] The mixed extract (complex herbal extract) of the present invention increases the expression of dermal hyaluronic acid synthase and decreases the expression of hyaluronic acid decomposition enzyme, thereby increasing the expression of hyaluronic acid, thereby increasing skin moisture retention, protecting the skin barrier, preventing skin aging, and suppressing wrinkle formation. Therefore, the composition of the present invention can be usefully used as a composition for moisturizing the skin, strengthening the skin barrier, inhibiting photoaging, or improving skin wrinkles.

[0051] In one embodiment of the present invention, the mixed extract (composite herbal extract) of the present invention suppresses an increase in epidermal thickness due to ultraviolet irradiation.

[0052] As previously mentioned, exposure to UV rays causes damage to skin tissue. Long-term exposure to UV rays can lead to a two- to threefold increase in epidermal thickness, and the epidermis is characterized by an increase in spinous cells and polymorphism of keratinocytes. Photoaging, in the case of photoaging, increases in the stratum corneum to protect the dermis, thickening the epidermis. Therefore, increased epidermal thickness indicates greater UV-induced skin damage.

[0053] The mixed extract (complex herbal extract) of the present invention suppresses the increase in epidermal thickness caused by UV irradiation, effectively inhibiting UV-induced skin damage and photoaging of the skin. Therefore, the composition of the present invention can be usefully used as a composition for inhibiting UV-induced skin damage or photoaging.

[0054] In one embodiment of the present invention, the mixed extract (composite herbal extract) of the present invention can significantly reduce the area of ​​wrinkles induced by ultraviolet rays, thereby improving skin wrinkles. Therefore, the composition of the present invention can be usefully used as a composition for improving skin wrinkles and inhibiting photoaging.

[0055] In one embodiment of the present invention, the mixed extract (complex herbal extract) of the present invention can increase the content of collagen and elastin, which are dermal matrix proteins.

[0056] Collagen, also known as collagen fibers, is a major component of the extracellular matrix, accounting for 85% to 90% of the dermis. Collagen is a protein produced in the form of procollagen by the action of fibroblasts. Newly synthesized procollagen undergoes an enzymatic reaction and is secreted into the extracellular space of skin cells, forming triple-helix microfibrils. These microfibrils then combine with leucine-rich small proteoglycans to form fibrils. These fibrils then assemble to form collagen fibers, giving skin its cohesiveness and elasticity. Collagen 1 (C1) accounts for the majority of skin collagen, and its primary functions include providing mechanical strength to the skin, providing connective tissue resistance and tissue cohesion, supporting cell adhesion, and inducing cell division and differentiation.

[0057] Elastin is an elastic fiber that accounts for approximately 3% to 4% of the dermis and affects the elasticity of the skin.

[0058] The mixed extract (complex herbal extract) of the present invention increases the content of collagen and elastin, which are dermal matrix proteins, thereby improving skin elasticity and inhibiting wrinkle formation. Therefore, the composition of the present invention can be usefully used as a composition for inhibiting skin damage caused by ultraviolet rays, inhibiting photoaging, or improving skin elasticity.

[0059] In one embodiment of the present invention, the mixed extract (composite herbal extract) of the present invention can increase the expression of collagen. More specifically, the mixed extract (composite herbal extract) of the present invention can increase the expression of collagen by decreasing the expression of matrix metalloproteinase 1 (MMP1), which decomposes collagen, and increasing the expression of tissue inhibitor of metalloproteinase 1 (TIMP1), which inhibits the activity of MMP1.

[0060] Matrix metalloproteinases (MMPs) are collagen-degrading enzymes. Increased MMP activity accelerates collagen breakdown. UV rays increase the expression of specific MMPs, increasing collagen breakdown and damaging the dermal layer's structure, ultimately contributing to skin aging and the formation of wrinkles.

[0061] While MMP is a representative enzyme that decomposes collagen, tissue inhibitor of metalloproteinase (TIMP) is an inhibitor that inhibits the activity of MMP.

[0062] The mixed extract (complex herbal extract) of the present invention reduces the expression of MMP1, which decomposes collagen, and increases the expression of TIMP1, which inhibits the activity of MMP1, thereby increasing collagen expression, thereby protecting the skin barrier, improving skin elasticity, suppressing skin wrinkles, and inhibiting photoaging. Therefore, the composition of the present invention can be usefully used as a composition for protecting the skin barrier, improving skin elasticity, improving skin wrinkles, or inhibiting photoaging.

[0063] The content of the mixed extract (complex herbal extract) of the present invention contained in the food composition of the present invention can be appropriately adjusted depending on the form of the food, the desired use, etc., and there is no special limitation. For example, the content of the mixed extract is 0.001 wt% to 80 wt% of the total food weight, or 0.01 wt% to 70 wt%, 0.1 wt% to 60 wt%, 0.1 wt% to 50 wt%, 0.1 wt% to 40 wt%, 0.1 wt% to 35 wt%, 0.1 wt% to 30 wt%, 1 wt% to 60 wt%, 1 wt% to 50 wt%, 1 wt% to 40 wt%, 1 wt% to 35 wt%, 1 wt% to 30 wt%, 5 wt% to 60 wt%, 5 wt% to 50 wt%, 5 wt% to 40 wt%, 5 wt% to 35 wt%, 5 wt% to 30 wt%, 10 wt% to 60 wt%, 10 wt% to 50 wt%, It may be, but is not limited to, 10 wt% to 40 wt%, 10 wt% to 35 wt%, 10 wt% to 30 wt%, 15 wt% to 60 wt%, 15 wt% to 50 wt%, 15 wt% to 40 wt%, 15 wt% to 35 wt%, or 15 wt% to 30 wt%.

[0064] When the composition comprising the mixed extract of perilla and perilla leaves of the present invention is manufactured into a food composition, it may include not only the extract as an active ingredient, but also ingredients commonly added during food manufacturing. The added ingredients include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. Examples of the carbohydrates mentioned above include monosaccharides such as glucose, fructose, etc.; disaccharides such as maltose, sucrose, oligosaccharides, etc.; and polysaccharides such as dextrin, cyclodextrin, etc., and sugar alcohols such as xylitol, sorbitol, and erythritol. As flavoring agents, natural flavoring agents [thaumatin, stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.)] and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is manufactured as a drink, citric acid, liquid fructose, sugar, glucose, acetic acid, malic acid, juice, Eucommia extract, jujube extract, licorice extract, etc. may be additionally included in addition to the extract of the present invention.

[0065]

[0066] According to another aspect of the present invention, the present invention provides a health functional food comprising the above-described food composition as an effective ingredient.

[0067] In this specification, the term "health functional food" refers to a food manufactured and processed with ingredients that have beneficial functions for the human body for the purpose of promoting and maintaining health, and may be used interchangeably with terms such as "dietary supplement," "health functional food," "food supplement," and "health food."

[0068] The content of the mixed extract (complex herbal extract) of the present invention contained in the health functional food of the present invention can be appropriately adjusted depending on the form of the food, the desired use, etc., and there is no special limitation. For example, the content of the mixed extract is 0.001 wt% to 90 wt% of the total weight of the health functional food, or 0.01 wt% to 87.5 wt%, 1 wt% to 87.5 wt%, 5 wt% to 87.5 wt%, 5 wt% to 85 wt%, 5 wt% to 80 wt%, 5 wt% to 70 wt%, 5 wt% to 60 wt%, 5 wt% to 50 wt%, 5 wt% to 30 wt%, 15 wt% to 87.5 wt%, 15 wt% to 85 wt%, 15 wt% to 80 wt%, 15 wt% to 70 wt%, 15 wt% to 60 wt%, 15 wt% to 50 wt%, 15 wt% to 30 wt%, 20 wt% to 87.5 wt%, 20 wt% 85 wt%, 20 wt% to 80 wt%, 20 wt% to 70 wt%, 20 wt% to 60 wt%, 20 wt% to 50 wt%, 20 wt% to 30 wt%, 30 wt% to 87.5 wt%, 30 wt% to 85 wt%, 30 wt% to 80 wt%, 30 wt% to 70 wt%, 30 wt% to 60 wt%, 30 wt% to 50 wt%, 50 wt% to 87.5 wt%, 50 wt% to 85 wt%, 50 wt% to 80 wt%, 50 wt% to 70 wt%, or 50 wt% to 60 wt%, and in the case of a health beverage composition, 1 wt% to 30 wt%, 1 wt% to 25 wt%, 1 wt% to 20 wt%, 1 wt% to 15 wt%, 1 wt% to 14 wt%, 5 wt% to 30 wt%, 5 wt% to 25 wt%, 5 wt% to 20 wt%, 5 wt% to 15 wt%, 5 wt% to 14 wt%, 8 wt% to 30 wt%, 8 wt% to 25 wt%, 8 wt% to 20 wt%, 8 wt% to 15 wt%, 8 wt% to 14 wt%, 10 wt% to 30 It may be, but is not limited to, 10 wt% to 25 wt%, 10 wt% to 20 wt%, 10 wt% to 15 wt%, or 10 wt% to 14 wt%.

[0069] The health functional foods described above may be beverages, meat, chocolate, food, confectionery, pizza, ramen, other noodles, gum, ice cream, alcoholic beverages, vitamin complexes, or health supplements.

[0070] The health functional food of the present invention comprises the food composition described above, and description of common matters between the two inventions is omitted to avoid excessive duplication in the present specification.

[0071]

[0072] According to another aspect of the present invention, the present invention provides a pharmaceutical composition for preventing or treating skin damage caused by ultraviolet rays, comprising a mixed extract of perilla and perilla leaves as an active ingredient.

[0073] In this specification, the term 'prevention' means any act of inhibiting or delaying skin damage caused by ultraviolet rays by administering a composition containing a mixed extract of perilla and perilla leaves according to the present invention as an active ingredient.

[0074] In this specification, the term 'treatment' means any action in which symptoms of skin damage caused by ultraviolet rays are improved or beneficially changed by administering a composition containing a mixed extract of perilla and perilla leaves according to the present invention as an active ingredient.

[0075] The pharmaceutical composition of the present invention comprises a pharmaceutically effective amount of a complex extract. The term "pharmaceutically effective amount" refers to an amount sufficient to achieve the efficacy or activity of the complex extract described above, and is not particularly limited as long as the purpose of the present invention is achieved.

[0076] The pharmaceutical composition of the present invention comprises a pharmaceutically acceptable carrier. Pharmaceutically acceptable carriers included in the pharmaceutical composition of the present invention are those commonly used in formulations, and include, but are not limited to, lactose, dextrose, sucrose, sorbitol, mannitol, starch, acacia gum, calcium phosphate, alginate, gelatin, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidone, water, syrup, and mineral oil. In addition to the above components, the pharmaceutical composition of the present invention may further comprise lubricants, wetting agents, sweetening agents, flavoring agents, emulsifiers, suspending agents, preservatives, and the like. Suitable pharmaceutically acceptable carriers and formulations are described in detail in Remignton's Pharmaceutical Sciences (19th ed., 1995).

[0077] The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, can be administered by intravenous injection, subcutaneous injection, intramuscular injection, etc.

[0078] The appropriate dosage of the pharmaceutical composition of the present invention can be prescribed in various ways depending on factors such as the formulation method, administration method, patient age, weight, sex, pathological condition, food, administration time, administration route, excretion rate, and response sensitivity. The typical dosage of the pharmaceutical composition of the present invention is within the range of 0.001-1000 mg / kg for adults. Furthermore, the human dosage can be converted based on animal testing.

[0079] The pharmaceutical composition of the present invention can be manufactured in a unit dose form or can be manufactured by inserting it into a multi-dose container by formulating it using a pharmaceutically acceptable carrier and / or excipient according to a method that can be easily performed by a person having ordinary skill in the art to which the present invention pertains, and the like. In this case, the formulation may be in the form of a solution, suspension, syrup or emulsion in an oil or aqueous medium, or in the form of an extract, powder, granule, tablet or capsule, and may additionally include a dispersing agent or stabilizer.

[0080]

[0081] According to another aspect of the present invention, the present invention provides a method for producing a composition for improving skin condition, which comprises a mixed extract of persimmon and persimmon leaf as an active ingredient, the method comprising the steps of: adding an extraction solvent to a mixture of persimmon and persimmon leaf in a weight ratio of 1-10:1-10 to extract; or the step of mixing a persimmon extract and a persimmon leaf extract in a weight ratio of 1-10:1-10.

[0082] In one embodiment of the present invention, the present invention may additionally include, after the above-described step, a step of concentrating the mixed extract obtained by the above-described step. More specifically, the present invention may additionally include a step of distilling or concentrating the mixed extract obtained by the above-described step under reduced pressure, and most specifically, the present invention may additionally include a step of concentrating the mixed extract obtained by the above-described step under reduced pressure, but is not limited thereto.

[0083] In one embodiment of the present invention, the present invention may additionally include a step of drying and powdering the mixed extract obtained by the above-described step. More specifically, the present invention may additionally include a step of powdering the mixed extract obtained by the above-described step by freeze-drying or spray-drying, and most specifically, the present invention may additionally include a step of powdering the mixed extract obtained by the above-described step by freeze-drying, but is not limited thereto.

[0084] The content regarding the extraction method of the mixed extract of persimmon and perilla leaves (composite herbal extract) included in the manufacturing method of the present invention is the same as the content regarding the extraction method of the mixed extract of persimmon and perilla leaves (composite herbal extract) in the above-described composition, and therefore, the description of the common content between the two is omitted to avoid excessive complexity of this specification.

[0085] The content regarding the mixed weight ratio of the persimmon and perilla leaf included in the manufacturing method of the present invention; or the mixed weight ratio of the persimmon extract and the perilla leaf extract is the same as the content regarding the extraction method of the mixed extract of persimmon and perilla leaf (composite herbal extract) in the above-described composition, and therefore, the description of the common content between the two is omitted to avoid excessive complexity of the present specification.

[0086]

[0087] The present invention relates to a composition for improving skin condition, comprising a mixed extract of perilla and perilla leaves as an active ingredient. The composition of the present invention has the efficacy of strengthening the skin barrier and inhibiting the formation of wrinkles, and thus can be usefully used as a composition for improving skin condition.

[0088]

[0089] Figure 1a shows the change in skin moisture retention due to dietary supply of complex herbal extract 1H, and Figure 1b shows the change in skin moisture retention due to dietary supply of complex herbal extract 2H.

[0090] Figure 1c shows the change in transepidermal water loss due to dietary supply of complex herbal extract 1H, and Figure 1d shows the change in transepidermal water loss due to dietary supply of complex herbal extract 2H.

[0091] Figure 2a shows the change in epidermal ceramide content due to dietary supply of complex herbal extract 1H, and Figure 2b shows the change in epidermal ceramide content due to dietary supply of complex herbal extract 2H.

[0092] Figure 2c shows changes in the mRNA and protein expression of ceramide synthase 3 (CerS3) due to dietary supply of complex herbal extract 1H, and Figure 2d shows changes in the mRNA and protein expression levels of ceramide synthase 3 (CerS3) due to dietary supply of complex herbal extract 2H.

[0093] Figure 2e shows changes in the mRNA expression level and protein expression level of ceramide synthase 4 (CerS4) due to dietary supply of complex herbal extract 1H, and Figure 2f shows changes in the mRNA expression level and protein expression level of ceramide synthase 4 (CerS4) due to dietary supply of complex herbal extract 2H.

[0094] Figure 3a shows changes in the mRNA expression level and protein expression level of filaggrin due to dietary supply of complex herbal extract 1H, and Figure 3b shows changes in the mRNA expression level and protein expression level of filaggrin due to dietary supply of complex herbal extract 2H.

[0095] Figure 3c shows the change in the content of free amino acids in the epidermis by dietary supply of complex herbal extract 1H, and Figure 3d shows the change in the content of free amino acids in the epidermis by dietary supply of complex herbal extract 2H.

[0096] Figure 3e shows the change in the content of individual free amino acids by dietary supply of complex herbal extract 1H, and Figure 3f shows the change in the content of individual free amino acids by dietary supply of complex herbal extract 2H.

[0097] Figure 3g shows changes in the mRNA expression level and protein expression level of PADI3 (peptidylarginine deiminase 3) due to dietary supply of complex herbal extract 1H, and Figure 3h shows changes in the mRNA expression level and protein expression level of PADI3 due to dietary supply of complex herbal extract 2H.

[0098] Figure 3i shows changes in the mRNA expression level and protein expression level of CASP14 (caspase-14) due to dietary supply of complex herbal extract 1H, and Figure 3j shows changes in the mRNA expression level and protein expression level of CASP14 due to dietary supply of complex herbal extract 2H.

[0099] Figure 4a shows the change in dermal hyaluronic acid content due to dietary supply of complex herbal extract 1H, and Figure 4b shows the change in dermal hyaluronic acid content due to dietary supply of complex herbal extract 2H.

[0100] Figure 4c shows changes in the mRNA expression level and protein expression level of hyaluronic acid synthase 2 (HAS2) due to dietary supply of complex herbal extract 1H, and Figure 4d shows changes in the mRNA expression level and protein expression level of hyaluronic acid synthase 2 (HAS2) due to dietary supply of complex herbal extract 2H.

[0101] Figure 4e shows changes in the mRNA expression level and protein expression level of hyaluronic acid decomposition enzyme 1 (Hyaluronidase 1; HYAL1) by dietary supply of complex herbal extract 1H, and Figure 4f shows changes in the mRNA expression level and protein expression level of hyaluronic acid decomposition enzyme 1 (Hyaluronidase 1; HYAL1) by dietary supply of complex herbal extract 2H.

[0102] Figure 5a shows a photograph of changes in epidermal thickness due to dietary supply of complex herbal extract 1H, and Figure 5b shows a photograph of changes in epidermal thickness due to dietary supply of complex herbal extract 2H.

[0103] Figure 5c shows numerical values ​​of changes in epidermal thickness due to dietary supply of complex herbal extract 1H, and Figure 5d shows numerical values ​​of changes in epidermal thickness due to dietary supply of complex herbal extract 2H.

[0104] Figure 6a shows a representative photograph of skin wrinkles after dietary supplementation of complex herbal extract 1H, and Figure 6b shows a representative photograph of skin wrinkles after dietary supplementation of complex herbal extract 2H.

[0105] Figure 6c shows the total wrinkle area after dietary supply of the complex herbal extract 1H, and Figure 6d shows the total wrinkle area after dietary supply of the complex herbal extract 2H.

[0106] Figure 7a shows the results of observing dermal matrix proteins using Masson's trichrome staining after dietary supply of complex herbal extract 1H, and Figure 7b shows the results of observing dermal matrix proteins using Masson's trichrome staining after dietary supply of complex herbal extract 2H.

[0107] Figure 7c shows the results of observing dermal matrix proteins using Verhoeff-van Gieson solution staining after dietary supply of complex herbal extract 1H, and Figure 7d shows the results of observing dermal matrix proteins using Verhoeff-van Gieson solution staining after dietary supply of complex herbal extract 2H.

[0108] Figure 8a shows changes in the mRNA (Col1a1, Col1a2) expression level and protein expression level of collagen 1 (COL1) by dietary supply of complex herbal extract 1H, and Figure 8b shows changes in the mRNA (Col1a1, Col1a2) expression level and protein expression level of collagen 1 (COL1) by dietary supply of complex herbal extract 2H.

[0109] Figure 8c shows changes in the mRNA expression level and protein expression level of matrix metalloproteinase 1 (MMP1) by dietary supply of complex herbal extract 1H, and Figure 8d shows changes in the mRNA expression level and protein expression level of matrix metalloproteinase 1 (MMP1) by dietary supply of complex herbal extract 2H.

[0110] Figure 8e shows changes in the mRNA expression level and protein expression level of tissue inhibitor of metalloproteinase 1 (TIMP1) by dietary supply of complex herbal extract 1H, and Figure 8f shows changes in the mRNA expression level and protein expression level of tissue inhibitor of metalloproteinase 1 (TIMP1) by dietary supply of complex herbal extract 2H.

[0111]

[0112] Hereinafter, the present invention will be described in more detail through examples. These examples are intended solely to illustrate the present invention more specifically, and it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples, in accordance with the gist of the present invention.

[0113]

[0114] Example

[0115]

[0116] (Throughout this specification, "%" used to indicate the concentration of a particular substance is (weight / weight) % for solid / solid, (weight / volume) % for solid / liquid, and (volume / volume) % for liquid / liquid, unless otherwise stated.)

[0117]

[0118] Experimental materials and methods

[0119] 1. Preparation of complex herbal extracts

[0120] Dried persimmon (Actinidia arguta) and perilla leaves (Perilla frutescens Britton) were purchased and used in the experiment. 90 g and 10 g of the persimmon and perilla leaves were mixed in a weight ratio (w / w) of 9:1, 800 mL of distilled water was added, the mixture was well stirred, and the mixture was refluxed at an extraction temperature of 85 to 95°C for 3 hours, after which the filtrate was separated. The separated filtrate was concentrated under reduced pressure at a temperature of 60°C or lower, and dextrin was added in a 1:1 ratio to the solid content, followed by powdering to obtain a complex herbal extract powder in a yield of about 90 to 100%. The complex herbal extract obtained by mixing persimmon and perilla leaves in a weight ratio of 9:1 was named 1H.

[0121]

[0122] In addition, 800 mL and 2000 mL of distilled water were added to 100 g of the dried persimmon and 100 g of perilla leaves, respectively, and stirred well, and then reflux extraction was performed at an extraction temperature of 85 to 95°C for 3 hours, after which the filtrates were separated, and this was repeated twice. Each filtrate was concentrated under reduced pressure at a temperature of 60°C or lower, and then dextrin was added at a 1:1 ratio to the solid content and powdered to obtain persimmon extract powder at a yield of about 90 to 100% and perilla leaf extract powder at a yield of about 20 to 30%. Thereafter, the persimmon extract powder and the perilla leaf extract powder were mixed at a weight ratio (w / w) of 4:1 and used in the experiment, and this complex herbal extract was named 2H.

[0123]

[0124] 2. Laboratory animals

[0125] Female hairless mice [Albino hairless mice (SKH-1)], 5-6 weeks old, were fed and adapted to a regular solid mixed diet for 1 week. After the 1-week adaptation period, the mice were divided into the normal control group without UV irradiation (UV- group), UV irradiation group (UV+ group), and the experimental groups (1H200 group, 1H400 group, 1H800 group, 2H200 group, 2H400 group, and 2H800 group) that were fed UV irradiation and 1H or 2H at a dose of 200 mg / kg (mg / kg body weight), 400 mg / kg, and 800 mg / kg, respectively. In addition, the 1H1% group and the 2H1% group, which are administered at a dose of 1% in diet, which are commonly used in animal experiments related to the development of health functional food materials, were set as comparison groups. During the breeding period, water and food / diet were freely available, and the animals were raised in a laboratory maintained under optimal environmental conditions of 24 ± 2℃ and 55 ± 10% humidity with a 12 hr / day light / dark cycle. The animal experiment protocol was approved by the Animal Ethics Committee of Kyung Hee University (Approval Number: KHGASP-22-247).

[0126]

[0127] 3. UV irradiation

[0128] From the first day of dietary supply, the back area (2 cm x 3 cm based on the center of the back) was irradiated three times a week using a UVB lamp (containing approximately 30% UVA: TL 20W / 12RS SLV, wavelength 290 nm to 390 nm, Phillips, Netherlands). The UV exposure dose was measured using a UV meter (VARIOCONTROL, Waldmann ver. 2.03, Germany). In week 1, 1 Minimal Erythema Dose (MED; 75 mJ / cm) 2 , 3 min) and 2 MED (150 mJ / cm) for 2 weeks 2, 6 min), 3 weeks 3 MED (225 mJ / cm 2 , 9 min), 4 MED (300 mJ / cm2) in weeks 4-10 2 , 12 minutes) was investigated.

[0129]

[0130] 4. Experimental diet

[0131] The UV- and UV+ groups were fed a regular diet (AIN-93G diet). The oral administration doses of 200 mg / kg, 400 mg / kg, and 800 mg / kg for 1H and 2H used in the previous study were converted to g / kg dry diet at levels of 0.24% to 0.96%, taking into account the average body weight (~30 g) and average daily food intake (~5 g) of 5-6 week-old SKH-1 mice for 10 weeks and the 50% excipient concentration of the sample, and were added to the diet and fed. In addition, the 1% dose of the diet for 1H and 2H was added to the diet at levels of 2% g / kg dry diet, taking into account the 50% excipient concentration of the sample. The difference in dry diet weight caused by the addition of the sample was adjusted by subtracting cornstarch. The dietary composition of each experimental group is shown in Table 1 below.

[0132]

[0133]

[0134] 1) AIN-93 Vitamin Mix #310025 (Dyets Inc., Bethlehem, PA)

[0135] 2) AIN-93G Salt Mix #210025 (Dyets Inc., Bethlehem, PA)

[0136] 3) Casein (nitrogen x 6.25), 870g / kg

[0137] 4)Calculated based on average weight (~30g) and average food intake (~5g) of hairless mice, and content of forming agent (50%)

[0138]

[0139] 5. Measurement of weight and food intake

[0140] To measure food efficiency ratio (FER), body weight, food supply, and food residue were measured once a week. FER was calculated by dividing body weight gain during the experimental period by total food intake.

[0141]

[0142] 6. Measurement of skin moisture retention and transepidermal moisture loss

[0143] Skin moisture retention and transepidermal water loss (TEWL) of the experimental animals were measured at 10 weeks using Corneometer CM 825 (Courage&Khazaka, Cologne, Germany) and Tewameter TM300 (Courage&Khazaka, Cologne, Germany), respectively. During the measurement, the temperature was maintained at 24 ± 2℃ and the humidity was 55 ± 10%. The same area on the back of the experimental animals was gently pressed three times, and the average value was recorded.

[0144]

[0145] 7. Separation of epidermis and dermis from experimental animals

[0146] After 10 weeks of experimental diet feeding, experimental animals were sacrificed by cervical dislocation, and skin tissues were collected from the dorsal area (2 cm x 3 cm from the mid-dorsal point) that had been irradiated with UV light. The skin tissues were soaked in a solution containing Hank's Balanced Salt Solution (HBSS, Gibco BRL, USA) and Dispase II (2.4 unit / mL, Roche, Germany) in a 1:1 (v / v) ratio and treated at 4°C for 16 h, after which the epidermis and dermis were separated.

[0147]

[0148] 8. Epidermal lipid extraction and ceramide content analysis

[0149] The separated epidermis was pulverized by adding PBS, and the lower layer obtained after centrifugation was dried using N2 gas and finally dissolved in chloroform:methanol (1:1, v / v) solution. Ceramide species in the lipids extracted from the epidermis were analyzed using a developing solution [1st / 2nd chloroform:methanol:water (40:10:1, v / v / v) with a 2.5 cm development (1st) and a 5 cm development (2nd); chloroform:methanol:acetic acid (47:2:0.5, v / v / v) with a 8.5 cm development (3rd); High-performance thin layer chromatography (HPTLC) was used to analyze the fractions using [n-hexane:diethyl ether:acetic acid (30:15:0.5, v / v / v) developed to 9.5 cm (4th)]. The content of each fractionated ceramide species was quantified at 254 nm using a TLC III scanner along with the corresponding standard substance after treatment with cupric acetate-phosphoric acid and color development. The results were expressed as μg / μg protein along with the protein quantification of the epidermal sample.

[0150]

[0151] 9. Analysis of epidermal free amino acid content

[0152] The epidermal tissue was pulverized in 0.05 M potassium phosphate buffer (pH 6.8), and trichloroacetic acid was added to a final concentration of 15%, and the mixture was left to stand at 4°C for 16 h. After removing proteins by centrifugation, the supernatant was filtered through a 0.2 μm filter prior to amino acid analysis. The concentrations of individual amino acids were analyzed by reverse-phase HPLC after derivatization with phenyl isothocyanate (PITC). A 10 μl volume sample was injected onto a Nova-Pak C18 column (3.9 X 300 mm, 4 μm, WAT011695 Waters, USA) and separated using solution A [140 mM sodium acetate trihydrate, 0.15% triethanolamine, 0.03% ethylene-diamine tetraacetic acid (EDTA), 6% acetonitrile] (pH 6.1) and solution B [60% acetonitrile, 0.015% EDTA] as the mobile phase. After maintaining solution A at 100% for 12 minutes, solution B was increased from 8% to 100% over 10 minutes and maintained for 3 minutes. After returning to the initial state of solution A at 100% in 1 minute, the concentration was maintained for 8 minutes. The flow rate was maintained at 1.0 ml / min until the B solution reached 100%, and then at 1.5 ml / min. The eluted amino acids were quantified at 254 nm using a variable wavelength detector (HP 1100 series, Agilent technologies, USA). The free amino acid content was expressed as μmol / mg protein along with the protein quantification of the epidermal sample.

[0153]

[0154] 10. Analysis of dermal hyaluronic acid content

[0155] PBS was added to the dermal tissue and pulverized. The hyaluronic acid concentration in the supernatant obtained by centrifugation was measured at 450 nm using a hyaluronic acid quantikine ELISA kit (E4626-100, BioVision, USA). The hyaluronic acid content was expressed as ng / mg protein, along with the protein quantification of the dermal sample used.

[0156]

[0157] 11. Measurement of epidermal thickness

[0158] 2 mm obtained from the dorsal area to visually measure the extent of epidermal proliferation 2 The skin tissue was fixed in neutral buffered formalin, sectioned into 8 μm thick sections, and stained with hematoxylin and eosin (H&E). The epidermal layer thickness was measured using an optical microscope, and the degree of epidermal proliferation was quantified.

[0159]

[0160] 12. Measurement of skin wrinkle formation

[0161] After anesthesia, a 6 mm inner diameter mold was placed on the back, and a silicone mixture prepared by adding approximately 1.5 g of light-bodied silicone (SilfloR, Flexico UK) and 1 drop each of catalyst and thinner was applied to the mold. After confirming complete drying, the mold was carefully removed from the skin, and the total area of ​​wrinkles was measured using a skin visioline VL650 skin burn analyzer (Courage&Khazaka, Cologne, Germany). In addition, photographs were taken and used as reference for observing macroscopic skin changes.

[0162]

[0163] 13. Histological analysis of dermal matrix protein content

[0164] After anesthetizing the animal, skin tissue was obtained and fixed in 10% formalin solution for more than 24 hours. Afterwards, it was washed, dehydrated, transparent, and infiltrated, fixed in paraffin, sectioned at a thickness of 5 μm, and stained as follows. In order to determine the quantitative change in collagen, a collagen fiber, Masson's trichrome (Masson's trichrome stain LG-6-solution; Sigma, St. Louis, MO, USA) staining (M&T staining method) was performed and observed using an optical microscope. In order to determine the quantitative change in elastin, an elastic fiber, it was first stained with the elastin stain solution in the elastin staining kit (Sigma, St. Louis, MO, USA), differentially washed with ferric chloride solution, stained with Verhoeff-van Gieson solution (VVG staining method), and observed using an optical microscope.

[0165]

[0166] 14. Protein expression analysis using Western blotting

[0167] RIPA buffer (20 mM Tris-HCl, pH 8, 150 mM NaCl, 10 mM NaPO4, 10% glycerol, 100 μM Na3VO4, 100 μM ammonium molybdate, 1% NP-40, 0.1% SDS) was added to epidermal or dermal tissues and stored at -20°C. After thawing, the tissues were ground and centrifuged to prepare the supernatant as a protein extract. The quantified proteins were electrophoresed on an SDS-PAGE gel and then adsorbed onto a nitrocellulose membrane.Metabolism-related enzymes and precursors of moisturizing-related indicators whose changes were identified in the experimental group [Ceramide production-related enzymes: CerS3 (H00204219-B01P, Abnova, Taipei, Taiwan), CerS4 (sc-376497, Santa Cruz Biotechnology, CA, USA); Free amino acid production-related precursors and enzymes: Filaggrin (GTX23137, GeneTex, Taipei, Taiwan), CASP14 (sc-4833, Santa Cruz Biotechnology), PADI3 (sc-55693, Santa Cruz Biotechnology); The cells were incubated with primary antibodies corresponding to hyaluronic acid production / degradation-related enzymes: HAS2 (sc-514737; Santa Cruz Biotechnology), HYAL1 (sc-101340, Santa Cruz Biotechnology)], or matrix proteins and degradation-related enzymes [Collagen 1 (COL1: #72026, Cell Signaling Technology, MA, USA), matrix metalloproteinase 1 (MMP1: #54376, Cell Signaling Technology), tissue inhibitor of metalloproteinase 1 (TIMP1: AB770, Merck Millipore, CA, USA)] for 16 hours at 4°C, followed by reaction with peroxidase-conjugated anti-IgG secondary antibody for 1 hour at room temperature, and then color development was confirmed with ECL hyperfilm.

[0168]

[0169] 15. mRNA expression analysis using RT-PCR

[0170] Total RNA was extracted and quantified from epidermal or dermal tissues using Trizol reagent (Gibco, USA), and cDNA was synthesized using the SuperScript III first-strand synthesis system (Invitrogen, USA). The synthesized cDNA was amplified using oligonucleotide primers specific for hydration-related indicators and production / degradation-related enzymes (ceramide production-related enzymes: CerS3, CerS4; free amino acid production precursors and enzymes: fillagrins, CASP14, PADI3; hyaluronic acid production / degradation-related enzymes: HAS2, HYAL1) or matrix proteins and degradation-related enzymes (COL1, MMP1, TIMP1) with a real-time multiplexing amplification system with SYBR Green (Qiagen) dye. The PCR products were quantitatively analyzed, and the mRNA expression level was expressed relative to GAPDH.

[0171]

[0172] 16. Statistical Analysis

[0173] The experimental results were statistically analyzed using the SPSS, ver. 26.0 program (statistical package for social science, IBM SPSS, Anmonk, NY, USA). The results are expressed as mean ± SEM (Standard Error of the Mean), and comparisons between each group were analyzed using one-way ANOVA and verified using Duncan's multiple comparison test at the p<0.05 level. In addition, changes between the two groups based on the UV+ group were analyzed using an unpaired Student's t-test, and significance was indicated at the p<0.05 level.

[0174]

[0175] Example 1. Changes in body weight, food intake, and food efficiency of experimental animals fed a complex herbal extract diet.

[0176] The initial body weight of each experimental group divided by the egg block method ranged from 23 g to 25 g without significant difference between groups. After 10 weeks of dietary supply, the average body weight of each experimental group ranged from 29.0 g to 33.5 g, and the average weekly weight gain for the 10 weeks of the UV- and UV+ groups, as well as the 1H and 2H experimental groups, did not differ between groups. In terms of food intake and food efficiency, there were no significant differences among all experimental groups fed the 1H or 2H sample, including the UV- and UV+ groups.

[0177] Thus, the dietary efficiency of all experimental groups was similar to that of the UV- and UV+ groups, confirming that the 10-week dietary supply of 1H and 2H dietary materials did not have a negative effect on body weight and food intake.

[0178]

[0179] Example 2. Changes in skin moisture retention and transepidermal water loss due to dietary supplementation of complex herbal extracts.

[0180] Changes in skin moisture retention due to dietary supplementation of complex herbal extracts are shown in Fig. 1a and Fig. 1b. Skin moisture retention in the UV+ group significantly decreased compared to the UV- group. On the other hand, skin moisture retention significantly increased compared to the UV+ group in all experimental groups in 1H and 2H, and increased compared to the UV+ group starting from the 1H200 and 2H200 groups, and increased to a level similar to the UV- group in the 1H1% and 2H1% groups.

[0181] Changes in transepidermal water loss (TEWL) following dietary supplementation with a complex herbal extract are shown in Fig. 1c and 1d. Transepidermal water loss (TEWL) in the UV+ group significantly increased compared to the UV- group. On the other hand, transepidermal water loss (TEWL) in all experimental groups at 1H and 2H significantly decreased compared to the UV+ group. It decreased from the 1H200 and 2H200 groups compared to the UV+ group, and decreased to the level of the UV- group in the 1H1% and 2H1% groups (Fig. 1c and 1d).

[0182] In this way, it was confirmed that the complex herbal extract of the present invention can help prevent skin moisture loss and skin barrier damage caused by ultraviolet rays and enable the skin barrier function to function normally by increasing skin moisture retention and decreasing transepidermal moisture loss through dietary supply of 1H and 2H.

[0183]

[0184] Example 3. Changes in epidermal ceramide content and ceramide synthase expression levels due to dietary supplementation of complex herbal extracts.

[0185] 3-1. Changes in epidermal ceramide content due to dietary supplementation of complex herbal extracts

[0186] The epidermal ceramide content by dietary supply of complex herbal extracts is shown in Fig. 2a and Fig. 2b. In the UV- group, the content of ceramide species Cer (ceramide) 1 to Cer6 was in the order of Cer1 > Cer2 > Cer6 > Cer4 > Cer5 > Cer3. On the other hand, the total Cer content in the UV+ group was significantly reduced compared to the UV- group, and in particular, the contents of Cer2, Cer4, and Cer6 were significantly reduced compared to the UV- group (Fig. 2a and Fig. 2b).

[0187] In the 1H experimental group, the total ceramide content increased in a 1H concentration-dependent manner, and from the 1H400 group, the total ceramide content increased more than the UV+ group, reaching a level similar to the UV- group in the 1H800 group. In particular, it was found that the contents of Cer2, Cer5, and Cer6 increased in a concentration-dependent manner to levels comparable to or higher than the UV- group. The content of Cer1, known as the main ceramide species of the epidermis, significantly increased only in the 1H800 group compared to the UV+ group, and the Cer3 content was similar to the UV+ group in all 1H experimental groups (Fig. 2a).

[0188] The total ceramide content of the 2H experimental group also increased in a concentration-dependent manner, exceeding the UV+ group level from the 2H200 group and reaching a maximum exceeding the UV- group in the 2H800 group. In particular, the content of Cer1 increased in a concentration-dependent manner, exceeding the UV- group from the 2H200 group and reaching a maximum in the 2H800 group. The content of Cer5 also increased in a concentration-dependent manner and reached a maximum in the 2H800 group. The content of Cer3 was significantly increased in the 2H800 group compared to the UV+ group, and Cer4 was significantly increased in the 2H200 group compared to the UV+ group. The content of Cer6 was significantly increased in all experimental groups at 2H compared to the UV+ group. On the other hand, the content of Cer2 was similar to that of the UV+ group in all experimental groups at 2H (Fig. 2b).

[0189]

[0190] 3-2. Changes in ceramide synthase expression due to dietary supplementation of complex herbal extracts

[0191] Ceramide synthase (CerS) is commonly involved in the initial production and regeneration of various ceramide species. While various CerS isozymes exist in different tissues, ceramide synthase 3 (CerS3) and ceramide synthase 4 (CerS4) are the major CerS isozymes in the epidermis. Changes in ceramide synthase expression levels following dietary supplementation of a complex herbal extract are shown in Figures 2c to 2f.

[0192] The CerS3 mRNA expression level of the UV+ group decreased compared to the UV- group, but the CerS3 protein expression levels were similar between the two groups, and the CerS3 protein expression levels of all experimental groups in 1H and 2H decreased compared to the UV+ group (Fig. 2c and 2d). The CerS4 mRNA expression level of the UV+ group was similar to the UV- group, but the CerS4 protein expression was decreased compared to the UV- group (Fig. 2e and 2f). The CerS4 mRNA expression levels and CerS4 protein expression levels of the 1H experimental group were similar to those of the UV+ group, whereas in the 2H experimental group, the mRNA expression levels and CerS4 protein expression levels increased overall compared to the UV+ group, and in particular, the 2H200 and 2H800 groups significantly increased compared to the UV+ group.

[0193]

[0194] In summary, in the UV+ group, the total content of ceramides, including Cer2, Cer4, and Cer6, decreased along with a decrease in the protein expression of CerS4, and in the 1H and 2H experimental groups, the total content of ceramides increased in a concentration-dependent manner. Specifically, in the 2H experimental group, the total content of ceramides, including Cer1, Cer5, and Cer6, increased from the 2H200 group compared to the UV+ group and was maximized to a level higher than the UV- group in the 2H800 group. This appears to be related to the increase in CerS4 mRNA expression level and CerS4 protein expression. On the other hand, in the 1H experimental group, the total content of ceramides, including Cer2, Cer5, and Cer6, increased from the 1H400 group compared to the UV+ group and was maximized to a level similar to the UV- group in the 1H800 group. This is thought to be caused by changes in the expression of enzymes other than CerS3 and CerS4.

[0195]

[0196] Accordingly, it was confirmed that the complex herbal extract of the present invention can prevent damage to the skin barrier by increasing the content of epidermal ceramide and help the skin barrier function to function normally.

[0197]

[0198] Example 4. Changes in filaggrin expression level, epidermal free amino acid content, and filaggrin-degrading enzyme expression level by dietary supplementation of complex herbal extracts.

[0199] 4-1. Changes in filaggrin expression levels due to dietary supplementation of complex herbal extracts

[0200] Free amino acids are produced by the breakdown of filaggrin, which occurs in several stages during the epidermal differentiation process. The expression levels of filaggrin mRNA and filaggrin protein by dietary supply of complex herbal extracts are shown in Figures 3a and 3b. In the UV+ group, the expression levels of filaggrin mRNA and filaggrin protein were significantly decreased compared to the UV- group. The expression levels of filaggrin mRNA and filaggrin protein in the 1H and 2H experimental groups increased in a concentration-dependent manner. In particular, the expression level of filaggrin protein in the 1H experimental group was significantly increased from the 1H400 group, and in the 2H experimental group from the 2H200 group compared to the UV+ group.

[0201] In this way, it was confirmed that the complex herbal extract of the present invention can increase the expression of filaggrin protein in the epidermis, thereby maintaining epidermal homeostasis and physiological acidity, and increasing the skin's moisture retention capacity, thereby helping the normal function of the skin barrier.

[0202]

[0203] 4-2. Changes in epidermal free amino acid content due to dietary supplementation of complex herbal extracts

[0204] Changes in epidermal free amino acid content following dietary supplementation of a complex herbal extract are shown in Figures 3c and 3d. Analysis results showed that while the difference in total free amino acid content between the experimental groups was not significant, the epidermal free amino acid content in the UV+ group decreased compared to the UV- group. Furthermore, the epidermal free amino acid content in the 1H and 2H experimental groups increased compared to the UV+ group.

[0205] Changes in the content of individual free amino acids by dietary supply of complex herbal extracts are shown in Figs. 3e and 3f. The individual free amino acids in the epidermis were arranged in descending order of content based on the UV- group, and the order of highest content was serine (SER) > glycine (GLY) > alanine (ALA) > arginine (ARG) > glutamine (GLN) > proline (PRO) > glutamic acid (GLU). Although there was some variation in the specific ranking, this was consistent with the results of a previous study that identified the above amino acids as the main free amino acids in the epidermis. In the 1H experimental group, the content of proline significantly increased in the 1H200 and 1H1% groups compared to the UV+ group (Fig. 3e). In the 2H experimental group, glycine, alanine, proline, and glutamic acid were significantly increased in several 2H experimental groups compared to the UV+ group (Fig. 3f).

[0206] In this way, it was confirmed that the complex herbal extract of the present invention can increase the content of free amino acids in the epidermis reduced by ultraviolet irradiation, normalize the composition of free amino acids in the epidermis, increase skin moisture retention, and help the normal function of the skin barrier.

[0207]

[0208] 4-3. Changes in the expression level of filaggrin-degrading enzymes by dietary supplementation with complex herbal extracts

[0209] Epidermal free amino acids are generated during the breakdown of filaggrin. The mRNA and protein expression levels of PADI3 (peptidylarginine deiminase 3) and CASP14 (caspase-14), proteins involved in the breakdown of filaggrin, are shown in Figures 3g to 3j. In the UV+ group, both the mRNA and protein expression levels of PADI3 and CASP14 decreased compared to the UV- group. On the other hand, most of the 1H and 2H experimental groups showed an increase in the mRNA and protein expression levels of PADI3 and CASP14 to levels higher than the UV+ group or similar to those of the UV- group.

[0210]

[0211] In this way, it was confirmed that the complex herbal extract of the present invention not only increases the expression of filaggrin, but also increases the expression of PADI3 (peptidylarginine deiminase 3) and CASP14 (caspase-14) involved in the decomposition process of filaggrin, thereby promoting the production of epidermal amino acids from filaggrin, and consequently strengthening the skin barrier and suppressing the formation of wrinkles.

[0212]

[0213] Example 5. Changes in dermal hyaluronic acid content, hyaluronic acid synthase, and hyaluronic acid decomposition enzyme expression levels by dietary supplementation with a complex herbal extract.

[0214] 5-1. Changes in dermal hyaluronic acid content due to dietary supplementation of complex herbal extracts

[0215] Changes in dermal hyaluronic acid content by dietary supplementation of complex herbal extracts are shown in Fig. 4a and Fig. 4b. The hyaluronic acid content in the UV+ group significantly decreased compared to the UV- group. In both the 1H and 2H experimental groups, the hyaluronic acid content increased in a concentration-dependent manner. The 1H experimental group significantly increased compared to the UV+ group from the 1H400 group, and also significantly increased compared to the UV- group in the 1H800 and 1H1% groups. In the 2H experimental group, the content significantly increased compared to the UV+ group from the 2H200 group, and also significantly increased compared to the UV- group in the 2H800 and 2H1% groups.

[0216]

[0217] 5-2. Changes in the expression level of hyaluronic acid synthase 2 (HAS2) due to dietary supplementation of complex herbal extracts.

[0218] Changes in the mRNA and protein expression levels of hyaluronic acid synthase 2 (HAS2), the main hyaluronic acid synthase in dermal fibroblasts, are shown in Figures 4c and 4d. In the UV+ group, both mRNA and protein expression decreased compared to the UV- group, while both HAS2 mRNA and protein expression increased in a dose-dependent manner in the 1H and 2H experimental groups. In each of the 1H and 2H experimental groups, the levels significantly increased from the 1H400 and 2H200 groups to levels higher than those in the UV+ group.

[0219]

[0220] 5-3. Changes in the expression level of hyaluronidase 1 (HYAL1) by dietary supplementation of complex herbal extracts

[0221] The mRNA and protein expression levels of hyaluronidase 1 (HYAL1), the main hyaluronic acid-degrading enzyme in dermal fibroblasts, are shown in Fig. 4e and Fig. 4f. HYAL1 protein expression in the UV+ group increased compared to the UV- group. HYAL1 mRNA expression was similar to that of the UV+ group in all groups except the 1H1% and 2H1% groups. On the other hand, HYAL1 protein expression decreased to the level of the UV- group in all 1H experimental groups, and in the 2H experimental group, HYAL1 protein expression decreased in a concentration-dependent manner, decreasing from the 2H400 group compared to the UV+ group, and decreasing to a level similar to that of the UV- group in the 2H800 and 2H% groups.

[0222]

[0223] In this way, it was confirmed that the complex herbal extract of the present invention increases the expression of dermal hyaluronic acid synthase and decreases the expression of hyaluronic acid decomposition enzyme, thereby increasing the expression of hyaluronic acid, thereby increasing skin moisture retention, protecting the skin barrier, preventing skin aging, and suppressing wrinkle formation.

[0224]

[0225] Example 6. Changes in epidermal thickness due to dietary supply of complex herbal extracts

[0226] The results of H&E staining to histologically evaluate the degree of epidermal proliferation are shown in Figs. 5a and 5b, and the numerical results are shown in Figs. 5c and 5d. As a result, the epidermal thickness of the UV+ group was significantly increased compared to the UV- group. This means that UV irradiation caused a decrease in skin moisture retention and an increase in transepidermal water loss, which secondaryly induced epidermal hyperproliferation and increased epidermal thickness. In both the 1H and 2H experimental groups, the epidermal thickness decreased in a concentration-dependent manner. The 1H experimental group showed a significant decrease compared to the UV+ group starting from the 1H400 group, and the 1H800 and 1H1% groups showed significant decreases similar to or lower than the UV- group (Figs. 5a and 5c). The 2H experimental group significantly decreased from the 2H200 group to below the UV+ group, and in the 2H800 group and 2H1% group, it decreased to a level similar to the UV- group (Fig. 5b and Fig. 5d).

[0227] In this way, it was confirmed that the complex herbal extract of the present invention effectively suppresses skin damage caused by ultraviolet rays by reducing the increase in epidermal thickness due to ultraviolet irradiation and suppresses photoaging of the skin.

[0228]

[0229] Example 7. Inhibitory effect of skin wrinkle formation by dietary supply of complex herbal extracts.

[0230] The decrease in skin moisture retention and the increase in transepidermal water loss due to UV irradiation secondary to epidermal hyperproliferation lead to wrinkle formation as well. Photographs of skin wrinkles on the back of mice are shown in Figures 6a and 6b. As a result, wrinkles in the UV+ group were deeper and longer than those in the UV- group. In contrast, wrinkles in the 1H and 2H experimental groups showed a dose-dependent decrease in depth and shortening.

[0231] The results of measuring the total wrinkle area of ​​skin tissue using a visiometer are shown in Fig. 6c and Fig. 6d. The total wrinkle area of ​​the UV+ group significantly increased compared to the UV- group, and in both the 1H and 2H experimental groups, the total wrinkle area significantly decreased compared to the UV+ group in a concentration-dependent manner. In the 1H experimental group, skin wrinkles significantly decreased from the 1H400 group to the UV+ group. In the 2H experimental group, wrinkles decreased compared to the UV+ group from the 2H200 group, and in the 2H800 group and 2H1% group, the wrinkle area decreased to the level of the UV- group.

[0232] In this way, it was confirmed that the complex herbal extract of the present invention has an effect of inhibiting the formation of skin wrinkles, and in particular, it inhibits photoaging of the skin by reducing wrinkles of the skin caused by ultraviolet rays.

[0233]

[0234] Example 8. Changes in dermal matrix protein content due to dietary supply of complex herbal extracts.

[0235] 8-1. Changes in collagen content due to dietary supplementation of complex herbal extracts

[0236] Collagen and elastin are matrix proteins in the dermis, filling the dermis to maintain elasticity and suppress wrinkle formation. The results of observing the collagen content and density of dermal tissue using Masson's trichrome staining are shown in Figures 7a and 7b. Collagen is observed as blue in Masson's trichrome staining. In the UV+ group, the collagen content and density of dermal tissue decreased compared to the UV- group. Collagen content and density increased in a concentration-dependent manner in the 1H and 2H experimental groups, reaching levels similar to those in the UV- group in the 1H800 and 2H800 groups.

[0237]

[0238] 8-2. Changes in elastin content due to dietary supplementation of complex herbal extracts

[0239] The results of observing the elastin content and density of dermal tissue using Verhoeff-van Gieson solution staining are shown in Figures 7c and 7d. The elastin content and density of dermal tissue also decreased in the UV+ group compared to the UV- group, and increased in a concentration-dependent manner in the 1H and 2H experimental groups, reaching a level similar to that of the UV- group in the 1H1% and 2H1% groups.

[0240]

[0241] In this way, it was confirmed that the complex herbal extract of the present invention has the effect of improving skin elasticity and inhibiting wrinkle formation by increasing the content of collagen and elastin, which are dermal matrix proteins, and in particular, inhibiting photoaging of the skin by reducing wrinkles of the skin created by ultraviolet rays.

[0242]

[0243] Example 9. Changes in dermal collagen content and collagen production-related enzyme expression levels due to dietary supplementation of complex herbal extracts.

[0244] 9-1. Changes in collagen expression due to dietary supplementation of complex herbal extracts

[0245] There are about 20 types of collagen in the body, and collagen 1 (COL1) is the main matrix protein in the dermis. COL1 has a triple helix structure formed by mixing polypeptides that are products of two collagen 1 α1 genes (Col1α1) and one collagen 1 α2 gene (Col1α2).

[0246] The changes in the mRNA (Col1a1, Col1a2) expression levels and protein expression levels of collagen 1 (COL1) are shown in Fig. 8a and Fig. 8b. In the UV+ group, the expression of Col1α1 and Col1α2 mRNA was similar to that of the UV- group, but the expression of collagen 1 protein, which is composed of three heterotrimeric polypeptides, was significantly reduced compared to the UV- group. In the 1H and 2H experimental groups, the mRNA expression levels of Col1α1 and Col1α2 and the expression levels of collagen 1 protein increased in a concentration-dependent manner. In particular, collagen 1 protein expression significantly increased from the 1H400 group in the 1H experimental group and from the 2H800 group in the 2H experimental group compared to the UV+ group.

[0247]

[0248] 9-2. Changes in the expression of matrix protein-degrading enzymes and matrix protein-degrading enzyme inhibitors by dietary supplementation with complex herbal extracts.

[0249] Collagen content is regulated by matrix metalloproteinases (MMPs), which are involved in collagen degradation, and tissue inhibitors of metalloproteinases (TIMPs), which inhibit the action of MMPs. Collagen 1 in the dermis is degraded by matrix metalloproteinase 1 (MMP1), and the activity of MMP1 is inhibited by TIMP1.

[0250] The changes in the mRNA and protein expression levels of matrix metalloproteinase 1 (MMP1) are shown in Fig. 8c and Fig. 8d. In the UV+ group, the mRNA and protein expression of MMP1 significantly increased compared to the UV- group. On the other hand, the mRNA expression of MMP1 decreased in a concentration-dependent manner in the 1H and 2H experimental groups. The protein expression of MMP1 decreased to the level of the UV- group in all experimental groups in 1H, and in the 2H experimental group, it decreased compared to the UV+ group from the 2H200 group, and decreased to the level below the UV- group in the 2H400, 2H800, and 2H1% groups.

[0251] Changes in the mRNA and protein expression levels of tissue inhibitor of metalloproteinase 1 (TIMP1) are shown in Figures 8e and 8f. In the UV+ group, both mRNA and protein expression levels of TIMP1 were similar to those in the UV- group, but in the 1H and 2H experimental groups, mRNA and protein expression of TIMP1 increased in a concentration-dependent manner. In particular, the protein expression of TIMP1 in the 1H1% group and the 2H800 group increased to levels higher than those in the UV- group.

[0252]

[0253] In this way, it was confirmed that the complex herbal extract of the present invention can reduce the expression of MMP1, which decomposes collagen, and increase the expression of TIMP1, which inhibits the activity of MMP1, thereby increasing the expression of collagen, and consequently protecting the skin barrier, improving skin elasticity, suppressing skin wrinkles, and suppressing photoaging.

[0254]

[0255] While specific aspects of the present invention have been described in detail above, it is clear to those skilled in the art that these specific descriptions are merely preferred implementation examples and that the scope of the present invention is not limited thereto.

Claims

1. A food composition for improving skin condition, comprising a mixed extract of perilla and perilla leaves as an active ingredient.

2. A pharmaceutical composition according to claim 1, wherein the extract is extracted with an organic solvent, water, or a mixed solvent thereof.

3. A food composition according to claim 1, wherein the improvement in skin condition is at least one selected from the group consisting of inhibition of photoaging, improvement of skin wrinkles, inhibition of skin damage caused by ultraviolet rays, skin moisturizing, improvement of skin elasticity, and strengthening of the skin barrier.

4. A food composition according to claim 1, wherein the mixed extract of persimmon and perilla leaves is obtained by mixing persimmon and perilla leaves in a weight ratio of 1-10:

1.

5. A food composition according to claim 1, wherein the mixed extract of persimmon and perilla leaves is a mixture of persimmon extract and perilla leaf extract in a weight ratio of 1-10:

1.

6. A food composition according to claim 1, wherein the food composition increases the expression of epidermal filaggrin.

7. A food composition according to claim 1, wherein the food composition increases the content of epidermal ceramide.

8. A food composition according to claim 1, wherein the food composition increases the expression of dermal hyaluronic acid.

9. Health functional food containing the food composition of paragraph 1 as an active ingredient.

10. A cosmetic composition for improving skin condition, comprising a mixed extract of perilla and perilla leaves as an active ingredient.

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