Fixing apparatus for repeated positioning imaging of animal skin window

Abstract

Disclosed in the present application is a fixing apparatus for repeated positioning imaging of an animal skin window. The present application relates to the technical field of medical instruments. In the case of ensuring the resolution of the imaging system, the present application not only simplifies the imaging steps, which reduces the difficulty of imaging, but also improves the quality of the resulting images. The apparatus comprises a skin window apparatus and a fixing apparatus, wherein the skin window apparatus comprises a support piece, a fixing ring, and an optical plastic sheet; the support piece comprises a connecting part and a bent part; an imaging hole is formed in the center of the connecting part; the fixing ring is arranged in the imaging hole; the optical plastic sheet is arranged in the fixing ring; a plurality of elongated holes arranged linearly are formed in the connection between the bent part and the connecting part; the support piece is made of a stainless steel material; the fixing apparatus comprises a fixing base, a limiting fixing assembly, a porous fixing plate, a pitching adjuster, a three-axis displacement stage, and a magnetic attraction base which are sequentially arranged from top to bottom; the connecting part of the support piece is connected to the upper surface of the fixing base, and the bent part of the support piece is suspended.

Description

A fixing device for repeated positioning imaging of animal skin windows Technical Field

[0001] The present application relates to the technical field of medical devices, and in particular to a fixing device for repeated positioning and imaging of animal skin windows. Background Art

[0002] With the rapid development of medical imaging technology, photoacoustic imaging technology, as a non-invasive imaging method, can perform high-resolution, high-sensitivity microvascular imaging, and can continuously monitor and quantitatively analyze the morphological structure of new blood vessels that are closely related to tumor development and metastasis. In addition, high-resolution photoacoustic microscopy combined with photoacoustic spectroscopy technology can perform photoacoustic functional imaging of physiological parameters closely related to tumors, such as blood oxygen saturation. However, due to the limitation of the imaging depth of photoacoustic microscopy, it is unable to image and monitor the blood vessels inside the tumor.

[0003] There are currently two main solutions: The first method is to use multimode optical fiber or reduce the numerical aperture of the lens to increase the imaging depth. The disadvantages of this method are: 1. It will change the size of the focused spot, resulting in reduced imaging resolution; 2. The focal depth of the imaging system is limited, and the uneven sample surface will cause some areas to be out of focus, affecting the image quality. The second method is to make a skin window in the animal's skin to inject tumor cells to reduce the sample thickness, and then fix the animal's skin window with a skin window fixing device to prevent the in vivo imaging from being affected by the animal's own breathing, heartbeat or incomplete anesthesia, causing the entire skin window to shake under the influence of the mouse, resulting in image misalignment and distortion, and even the area of ​​interest may be completely moved out of the microscope imaging field. The disadvantages of this method are: 1. Since the skin window devices are supported on the mouse skin in an unfolded manner, the edges of the skin window devices are prone to warping, resulting in the skin window device and the skin window fixing device being unable to fit tightly when the focal length of the imaging system is short, thereby blocking the imaging system and affecting imaging; 2. The existing skin window device material is titanium alloy, but titanium alloy is expensive and difficult to process. The surface of the finished product is rough, and the toughness is insufficient and the bending resistance is weak; 3. The existing skin window fixing device is fixed to the platform by bolts and cannot move freely. In actual imaging, it is often necessary to flexibly change the imaging area. Technical issues

[0004] 1. Since the skin window devices are supported on the mouse skin in an unfolded manner, the edges of the skin window devices are prone to warping, resulting in the skin window device and the skin window fixing device being unable to fit tightly when the focal length of the imaging system is short, thereby blocking the imaging system and affecting imaging; 2. The existing skin window device material is titanium alloy, but titanium alloy is expensive and difficult to process. The surface of the finished product is rough, and the toughness is insufficient and the bending resistance is weak; 3. The existing skin window fixing device is fixed to the platform by bolts and cannot move freely. In actual imaging, it is often necessary to flexibly change the imaging area. Technical Solutions

[0005] The embodiments of the present application provide a fixing device for repeated positioning imaging of animal skin windows, which not only simplifies the imaging steps and reduces the imaging difficulty, but also improves the quality of the imaging image while ensuring the resolution of the imaging system.

[0006] To achieve the above-mentioned purpose, an embodiment of the present application provides a fixing device for repeated positioning and imaging of animal skin windows, including a skin window device and a fixing device; the skin window device includes a supporting plate, a fixing ring and an optical plastic sheet; the supporting plate includes a connecting portion and a bending portion; an imaging hole is provided at the center of the connecting portion; a fixing ring is provided in the imaging hole; an optical plastic sheet is provided in the fixing ring; a plurality of long holes arranged in a straight line are provided at the connection between the bending portion and the connecting portion; the supporting plate is made of stainless steel; the fixing device includes a fixed base, a limiting fixing assembly, a multi-porous fixing plate, a pitch adjuster, a three-axis displacement platform and a magnetic base arranged in sequence from top to bottom; the connecting portion of the supporting plate is connected to the upper surface of the fixed base, and the bending portion of the supporting plate is suspended in the air.

[0007] Furthermore, the bending portion is in the shape of an arc plate, and a plurality of weight-reducing holes are provided on the bending portion.

[0008] Furthermore, the optical plastic sheet is made of PVC optical film.

[0009] Furthermore, the porous plate is made of PMMA; the porous plate is provided with a plurality of countersunk holes and threaded holes arranged in an alternating manner.

[0010] Furthermore, the support plate is provided with three first connection holes, and the three first connection holes are located on the outer periphery of the support plate and the line connecting the three first connection holes forms an isosceles triangle; the fixed base is a rectangular plate; a center hole and multiple second connection holes are provided on the fixed base; the center hole corresponds to the position of the imaging hole on the support plate; the positions of the multiple second connection holes correspond one-to-one to the positions of the multiple first connection holes, and the multiple second connection holes are all arranged near the edge of the long side of the fixed base; the bolts are fastened to the fixed base after passing through the first connection holes.

[0011] Furthermore, the support sheet is provided with a plurality of suture hole groups for suturing and fixing the support sheet to the skin of the mouse; the suture hole groups are located at the edge of the connecting portion.

[0012] Furthermore, the fixing ring includes an inner ring surface, a middle ring surface and an outer ring surface; the middle ring surface is higher than the inner ring surface and the outer ring surface; the optical plastic sheet is mounted on the inner ring surface and fixed by a retaining spring; the supporting sheet is connected to the outer ring surface.

[0013] Furthermore, the limit fixing assembly includes two limit fixers arranged opposite to each other; a height adjustment assembly is also provided between the fixed base and the limit fixer; the height adjustment assembly includes two height adjustment columns; threaded holes are provided in the height adjustment columns; a third connecting hole is provided on the fixed base; a fourth connecting hole is provided on the limit fixer; the upper end of the height adjustment column is connected to the third connecting hole through a screw, and the lower end is inserted into the fourth connecting hole.

[0014] Furthermore, the pitch adjuster can respectively achieve adjustment of ±15° in two vertical planes.

[0015] Furthermore, the three-axis translation stage includes an xy two-axis translation stage and a z-axis translation stage connected to the lower part of the xy two-axis translation stage; the xy two-axis translation stage is connected to the pitch adjuster; and the z-axis translation stage is connected to the magnetic base. Beneficial effects

[0016] 1. The fixing device for repeated positioning and imaging of animal skin windows in the embodiment of the present application reduces the overall edge length by cutting key-length strip holes at the bending part of the support member, thereby greatly improving the bending ability of the skin window device while ensuring its required strength, and solving the technical problem in the prior art that the skin window is too high after being unfolded and affects high-resolution short-focus imaging.

[0017] 2. The support parts in the fixing device for repeated positioning imaging of animal skin windows in the embodiment of the present application are made of stainless steel with stronger toughness and more fine processing. Compared with the titanium alloy material in the prior art, the support parts in the embodiment of the present application have the advantages of low cost, high-efficiency processing and wider applicability.

[0018] 3. The fixing device for repeated positioning imaging of animal skin windows in the embodiment of the present application uses PVC optical film as the observation window material. Compared with the use of glass sheets, the PVC optical film in the embodiment of the present application greatly enhances the transmitted acoustic signal while ensuring excellent light transmittance, making it possible to achieve high-resolution photoacoustic imaging.

[0019] 4. The fixing device for repeated positioning and imaging of animal skin windows in the embodiment of the present application is portable and adjustable in height by setting a height adjustment column between the fixed base and the limit fixture and then cooperating with a screw-in stud.

[0020] 5. The fixing device for repeated positioning imaging of animal skin windows in the embodiment of the present application achieves precise leveling of the imaging area of ​​interest by setting a pitch adjuster, making it possible to obtain uniform high-quality imaging results.

[0021] 6. The fixing device for repeated positioning and imaging of animal skin windows in the embodiment of the present application is provided with a magnetic base under the three-axis translation stage. Compared with the prior art in which the fixing device is directly connected to the breadboard by screws, the embodiment of the present application can flexibly move the object under test to the light outlet of the imaging instrument, quickly find the imaging area of ​​interest, and then lock the magnetic base to achieve fixation, and then cooperate with the multi-axis adjustment stage to achieve fine adjustment of the imaging area, which greatly simplifies the operation steps and improves practicality.

[0022] 7. The fixing device for repeated positioning and imaging of animal skin windows in the embodiment of the present application combines a three-axis translation stage with a pitch adjuster and a magnetic base, thereby achieving manual coarse adjustment and fine adjustment of the translation stage, and the operation is quick and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0024] FIG1 is a schematic diagram of the three-dimensional structure of a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application at one angle;

[0025] FIG2 is a schematic diagram of the three-dimensional structure of the fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application from another angle;

[0026] FIG3 is a schematic diagram of the connection structure between the skin window device and the fixing device in the fixing device for repeated positioning imaging of animal skin windows according to an embodiment of the present application;

[0027] FIG4 is a schematic diagram of the three-dimensional structure of a support plate in a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application;

[0028] FIG5 is a schematic diagram of the three-dimensional structure of a fixing ring in a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application;

[0029] FIG6 is a schematic diagram of the three-dimensional structure of a fixing base in a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application;

[0030] FIG7 is a schematic diagram of the three-dimensional structure of a height adjustment column in a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application;

[0031] FIG8 is a schematic diagram of the three-dimensional structure of a limiter fixer in a fixing device for repeated positioning imaging of an animal skin window according to an embodiment of the present application. Modes for Carrying Out the Invention

[0032] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0033] In the description of this application, it should be understood that the terms "center", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application.

[0034] In the description of this application, it should be noted that, unless otherwise clearly specified and limited, the terms "install", "connect", and "connect" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0035] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature specified as "first" or "second" may explicitly or implicitly include one or more of such features. Throughout this application, unless otherwise specified, "plurality" means two or more.

[0036] 1 and 2 , an embodiment of the present application provides a fixing device for repeated positioning imaging of animal skin windows, including a skin window device 1 and a fixing device 2. The embodiment of the present application improves the structure and materials of the skin window device 1 and the fixing device 2 while ensuring the resolution of the imaging system. On the skin window device 1, by selecting low-cost and easy-to-process 304 stainless steel as the base, the overall edge length is reduced by laser cutting at the bend of the skin window device 1, and the bending ability of the skin window device 1 is greatly improved while ensuring the required strength. On the fixing device 2, by adding a multi-axis translation stage and a pitch translation stage, the sample position and horizontality can be adjusted in time to obtain the best image quality. At the same time, by adding a magnetic device at the bottom of the device, the imaging position can be freely adjusted. A matching acrylic fixing plate is provided to realize the linkage combination of multiple devices.

[0037] 3 , the leather window device 1 includes a supporting plate 11 , a fixing ring 12 , an optical plastic sheet 13 and a retaining spring 14 .

[0038] 4 , the support sheet 11 includes a connecting portion 111 and a bending portion 112. The shape of the connecting portion 111 is similar to an I-shape, and an imaging hole 113 is provided in the center thereof. The imaging hole 113 is a circular hole with a diameter of 14 mm. The imaging hole 113 serves as an exposed position of the animal skin to facilitate imaging. Three first connecting holes 114 for fixing the support sheet 11 are provided on the periphery of the imaging hole 113. The diameter of the first connecting hole 114 is 1.7 mm. One of the first connecting holes 114 is located on the right side of the imaging hole 113, and the other two first connecting holes 114 are located on the left side of the imaging hole 113, and the connecting lines of the three first connecting holes 114 form an isosceles triangle.

[0039] The support plate 11 is also provided with multiple groups of suture holes 115 for suturing and fixing the skin of the mouse. The suture hole groups 115 are located at the edge of the connecting portion 111. Specifically, a group of suture holes 115 is provided on the upper and lower sides of the first connecting hole 114 on the right side, and a group of suture holes 115 is provided on the upper side of the first connecting hole 114 on the upper left side and on the lower side of the first connecting hole 114 on the lower left side. The suture hole groups 115 include multiple suture holes 116. The number of suture holes 116 is set according to specific needs, for example, three or four. The diameter of the suture holes 116 is 0.9 mm.

[0040] The bending portion 112 is in the shape of an arc-shaped plate, and a plurality of elongated holes 117 arranged in a straight line are provided at the connection between the bending portion 112 and the connecting portion 111, for example, nine. The nine elongated holes 117 are symmetrically arranged relative to the center line of the support sheet 11. The extension direction of the elongated holes 117 is consistent with the layout direction, and the length of the elongated holes 117 is 2.5 mm. In this way, the difficulty of bending can be reduced while ensuring a certain strength here, providing the possibility for subsequent continuous imaging. In addition, three weight-reducing holes 118 are provided on the bending portion 112. In this way, the weight can be reduced and the overall strength of the support sheet 11 can be improved, thereby extending its service life.

[0041] Since existing window devices are generally made of titanium alloy, which is expensive, difficult to process, has a rough surface after processing, and is not tough enough and has poor bending resistance, the support sheet 11 in this application is made of 304 stainless steel, which is tougher and can be processed more finely, and is cut by laser, achieving low cost, high efficiency and wider applicability.

[0042] 3 and 5 , a fixing ring 12 is provided in the imaging hole 113. The fixing ring 12 is a circular ring structure with a high middle and low inner and outer sides. Specifically, the fixing ring 12 includes an inner ring surface 121, a middle ring surface 122 and an outer ring surface 123, and the middle ring surface 122 is higher than the inner ring surface 121 and the outer ring surface 123. The outer diameter of the outer ring surface 123 is 15 mm and the inner diameter is 14 mm; the outer diameter of the middle ring surface 122 is 14 mm and the inner diameter is 12 mm; the outer diameter of the inner ring surface 121 is 12 mm and the inner diameter is 11 mm. The outer ring surface 123 is used to snap into contact with the support sheet 11. The inner ring surface 121 is used to support the optical plastic sheet 13. In order to facilitate processing, the fixing ring 12 is cut from a stainless steel cylinder with a diameter of 15 mm and a thickness of 0.8 mm.

[0043] Referring to Figures 3 and 4 , the optical plastic sheet 13 is mounted on the inner annular surface 121 and secured by a circlip 14. Circlip 14 is gripped and installed into the retaining ring 12 using circlip pliers, securing the optical plastic sheet 13. Since the creation of the skin window requires exposing the mouse's inner epidermis, to protect the mouse's skin from prolonged exposure to air, which can lead to inflammation or even death, and to ensure long-term observation and imaging, after preliminary testing of various optical plastics, a PVC (polyvinyl chloride) film protective material was selected, given its best impedance match to water and the animal's body. This provides both an imaging window and animal protection. This optical film is not only suitable for photoacoustic imaging, but also achieves excellent imaging results for other optical imaging systems.

[0044] 1 and 2 , the fixing device 2 includes a fixing base 21 , a height adjustment assembly, a position limiting fixing assembly, a porous fixing plate 24 , a pitch adjuster 25 , a three-axis translation stage and a magnetic base 26 , which are arranged in sequence from top to bottom.

[0045] 3 and 6 , the fixed base 21 is connected to the lower part of the support sheet 11, and after the connection, the bent portion 112 of the support sheet 11 is suspended. The fixed base 21 is a rectangular plate. A center hole 211, three second connection holes 212 and two third connection holes 213 are provided on the fixed base 21. The center hole 211 corresponds to the position of the imaging hole 113 on the support sheet 11. The three second connection holes 212 are all 2 mm, and their positions correspond one-to-one to the positions of the three first connection holes 114 on the support sheet 11. The three second connection holes 212 are all set close to the edge of the long side of the fixed base 21. The bolts pass through the first connection hole 114 and the second connection hole 212 in sequence and are fastened with nuts. In this way, the support sheet 11 can be fixedly connected to the fixed base 21. The two third connection holes 213 are both M3 countersunk holes and are located on both sides of the center hole 211. The third connection hole 213 is used to connect the height adjustment component.

[0046] 7 , the height adjustment assembly includes two height adjustment columns 22. The height adjustment columns 22 are formed by a square column with 0.5mm bevels at both ends and 45° bevels, and an M3 threaded hole is opened in the middle to cooperate with the fixed base 21 to achieve height adjustment.

[0047] 3 and 8 , the lower part of the height adjustment assembly is connected to the limit fixing assembly. The limit fixing assembly includes two limiter fixtures 23. The two limiter fixtures 23 are divided into two on the left and right and are mirror-image structures. A fourth connecting hole 231 is provided on the right side of the upper surface of the limiter fixture 23, a fifth connecting hole 232 is provided on the left side, and an M3 threaded hole 233 is provided on the front side or the rear side. The fourth connecting hole 231 is adapted to the shape of the height adjustment column 22, and the height adjustment column 22 can be embedded in the fourth connecting hole 231, and the top screw screwed into the M3 threaded hole 233 on the side is used to prevent the height adjustment column 22 from loosening. The fifth connecting hole 232 is an M6 countersunk hole, which is fixed with an M6 screw and a porous fixing plate 24.

[0048] Referring to Figure 1, a porous fixing plate 24 is attached below the stopper fixture 23. Made of PMMA (polymethyl methacrylate), this plate is lightweight and transparent compared to stainless steel, allowing for easy observation of the internal screws and the underlying stage. The plate's surface features alternating M6 countersunk holes and M6 threaded holes, enabling multiple fixing options to accommodate diverse imaging environments. The M6 ​​countersunk holes, coupled with M6 studs, allow for secure connection to the stage.

[0049] Referring to Figure 2 , a pitch adjuster 25 is connected below the porous fixing plate 24. The pitch adjuster 25 can adjust the pitch by ±15° in two vertical planes. Because photoacoustic imaging uses motor scanning and has a shallow depth of focus, it is necessary to ensure that objects in the imaging area are approximately in the same plane. Therefore, the provision of the pitch adjuster 25 enables real-time adjustment, significantly improving imaging quality. The porous fixing plate 24 is applicable not only to the embodiments of this application but also to all other inventions requiring this plate.

[0050] A three-axis translation stage is connected below the pitch adjuster 25. This stage allows for displacement adjustment of the scanned object, facilitating selection of imaging regions of interest and achieving precise imaging. Specifically, the three-axis translation stage includes an xy-axis translation stage 27 and a z-axis translation stage 28 connected to the bottom of the xy-axis translation stage 27. The xy-axis translation stage 27 is connected to the pitch adjuster 25, while the z-axis translation stage 28 is connected to the magnetic base 26.

[0051] After all the aforementioned components are assembled, the integrated window fixture can be moved on the metal imaging platform, facilitating platform removal and securement. This also makes it easier to use the magnetic base for rough positioning during imaging, with the three-axis translation stage then performing precise positioning, making imaging more convenient. The pitch adjuster 25, magnetic base 26, xy-axis translation stage 27, and z-axis translation stage 28 are all conventionally used, and their structures will not be described in detail here.

[0052] The embodiments of this application have been verified through multiple experiments. The skin window device described herein was fabricated on mice. When the mouse was freely moving, the support member in the skin window device was extended to support the mouse's back. During photoacoustic imaging, the support member closed (i.e., the bent portion bent downward), ensuring unobstructed imaging. Furthermore, in conjunction with a series of adjustment mechanisms, the skin window support member of the present invention simplified the imaging process while achieving excellent image quality. Experiments have demonstrated that the skin window support member of the present invention can be bent multiple times, ensuring continuous imaging.

[0053] The above are only specific embodiments of the present application, but the scope of protection of the present application is not limited thereto. Any changes or replacements within the technical scope disclosed in this application should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. A fixing device for repeated positioning imaging of animal skin windows, characterized in that, it includes a skin window device and a fixing device; The skin window device includes a support sheet, a fixing ring and an optical plastic sheet; the support sheet includes a connecting portion and a bending portion; an imaging hole is provided at the center of the connecting portion; a fixing ring is provided in the imaging hole; an optical plastic sheet is provided in the fixing ring; a plurality of long holes arranged in a straight line are provided at the connection between the bending portion and the connecting portion; the support sheet is made of stainless steel; The fixing device includes a fixing base, a limit fixing component, a porous fixing plate, a pitch adjuster, a three-axis displacement stage and a magnetic suction base which are arranged in sequence from top to bottom; the connecting portion of the support sheet is connected to the upper surface of the fixing base, and the bending portion of the support sheet is suspended.

2. The fixing device for repeated positioning imaging of animal skin windows according to claim 1, characterized in that, the bending portion is an arc-shaped plate, and a plurality of weight-reducing holes are provided on the bending portion.

3. The fixing device for repeated positioning imaging of animal skin windows according to claim 2, characterized in that, the optical plastic sheet uses a PVC optical film.

4. The fixing device for repeated positioning imaging of animal skin windows according to claim 3, characterized in that, the porous plate is made of PMMA material; a plurality of countersunk holes and threaded holes are provided on the porous plate and are arranged alternately.

5. The fixing device for repeated positioning imaging of animal skin windows according to claim 4, characterized in that, three first connection holes are further provided on the support sheet, and the three first connection holes are located on the outer periphery of the support sheet and the connection line of the three first connection holes forms an isosceles triangle; the fixing base is a rectangular plate; a center hole and a plurality of second connection holes are provided on the fixing base; the position of the center hole corresponds to the position of the imaging hole on the support sheet; the positions of the plurality of second connection holes correspond to the positions of the plurality of first connection holes one by one, and the plurality of second connection holes are all arranged close to the edge of the long side of the fixing base; bolts pass through the first connection holes and are fastened to the fixing base.

6. The fixing device for repeated positioning imaging of animal skin windows according to claim 5, characterized in that, a plurality of suture hole groups for suturing and fixing with the skin of a mouse are further provided on the support sheet; the suture hole groups are located at the edge of the connecting portion.

7. The fixing device for repeated positioning imaging of animal skin windows according to claim 6, characterized in that, the fixing ring includes an inner ring surface, a middle ring surface and an outer ring surface; the middle ring surface is higher than the inner ring surface and the outer ring surface; the optical plastic sheet is installed on the inner ring surface and is fixed by a snap ring; the support sheet is connected to the outer ring surface.

8. The fixing device for repeated positioning imaging of animal skin windows according to claim 7, characterized in that, The limiting and fixing assembly includes two limiting fixators arranged oppositely; a height adjusting assembly is further provided between the fixed base and the limiting fixator; the height adjusting assembly includes two height adjusting columns; threaded holes are provided in the height adjusting columns; a third connection hole is provided on the fixed base; a fourth connection hole is provided on the limiting fixator; the upper end of the height adjusting column is connected to the third connection hole through a screw rod, and the lower end is inserted into the fourth connection hole.

9. The fixing device for repeated positioning imaging of animal skin windows according to claim 8, characterized in that, the pitching adjuster can respectively achieve adjustments of ±15° in two vertical planes.

10. The fixing device for repeated positioning imaging of animal skin windows according to claim 9, characterized in that, the three-axis displacement stage includes an xy two-axis displacement stage and a z-axis displacement stage connected to the lower part of the xy two-axis displacement stage; the xy two-axis displacement stage is connected to the pitching adjuster; the z-axis displacement stage is connected to the magnetic adsorption base.

Images