Multi-channel molecular diagnostic cartridge

Abstract

The present invention relates to a multi-channel molecular diagnostic cartridge comprising: a housing having an upper case and a lower case; a sample injection part that is formed recessed in a substrate of the lower case and receives samples that flow in through an injection opening formed in the upper case; a plurality of chambers formed recessed in the substrate of the lower case; and a plurality of transfer flow paths that extend between the sample injection part and the plurality of chambers. The transfer flow paths correspond one-to-one to the chambers connected to the transfer flow paths and can move the samples in the sample injection part to the corresponding chambers.

Description

Multichannel molecular diagnostic cartridge

[0001] This invention relates to a multi-channel molecular diagnostic cartridge.

[0002] Devices and methods for analyzing fluid samples are required in diverse fields, including environmental monitoring, food testing, and medical diagnostics. Previously, performing tests according to established protocols required skilled laboratories to manually perform various steps, including reagent injection, mixing, separation and transfer, reaction, and centrifugation. This process often resulted in errors in test results.

[0003] In the process of examining a sample, it is very important to ensure that the sample and the reagents used for examining the sample are not affected by external factors and that the exact amount is used each time to obtain accurate and reproducible results.

[0004] Miniaturized and automated equipment capable of rapid sample analysis has been developed. In particular, portable fluid analysis cartridges allow rapid, location-independent analysis of test substances. However, conventional cartridges are limited in their capacity, allowing testing of a limited number of samples, such as a single sample. Furthermore, they only allow qualitative analysis based on color changes, limiting accuracy. This makes it difficult to simultaneously identify multiple infectious diseases with similar symptoms, and increases the cost of infectious disease identification.

[0005] Therefore, there is a need to improve the structure and functionality of portable cartridges to enable simultaneous testing of multiple samples. Furthermore, there is a need to reduce the number of steps and costs involved in the overall testing process by enabling multiple liquid-based molecular diagnostic tests to be performed with a single sample injection.

[0006] The technology underlying this application is disclosed in Korean Patent No. 10-1726181.

[0007] This application was supported by Chungcheongbuk-do Province, and the research on this application was conducted through the project "Development of an ultra-small multi-molecular diagnostic system for field diagnosis" (Research management organization: Chungbuk Technopark, Research project name: Local government-Next-generation in vitro diagnostic device technology development support project, Host organization: Suzentech Co., Ltd., Contribution ratio: 1 / 1).

[0008] The present invention is intended to solve the problems of the above-mentioned prior art, and the purpose of the present invention is to provide a multi-channel molecular diagnostic cartridge that can perform various types of molecular diagnostics with a single sample deployment.

[0009] In addition, the purpose of this center is to provide a multi-channel molecular diagnostic cartridge that can be applied to a network-based healthcare information sharing system when connected to a smart device.

[0010] In addition, the purpose of this center is to provide a multi-channel molecular diagnostic cartridge that enables rapid diagnosis of infectious diseases with similar symptoms but different prescriptions, thereby enabling rapid and accurate diagnosis in the event of a large-scale infectious disease outbreak.

[0011] However, the technical tasks to be achieved by the embodiments of the present invention are not limited to the technical tasks described above, and other technical tasks may exist.

[0012] As a technical means for achieving the above technical task, a multi-channel molecular diagnostic cartridge according to one embodiment of the present invention comprises: a housing having an upper case and a lower case; a sample injection unit formed by being recessed from a substrate of the lower case and receiving a sample through an injection opening formed in the upper case; a plurality of chambers formed by being recessed from the substrate of the lower case; and a plurality of delivery channels extending between the sample injection unit and the plurality of chambers, wherein each of the delivery channels may be provided in one-to-one correspondence with each chamber connected to the delivery channel so as to move a sample in the sample injection unit to the corresponding chamber.

[0013] In addition, according to one embodiment of the present invention, the device may further include a through hole extending between the upper surface of the substrate and one surface of the lower case corresponding to the chamber; and a sealing channel extending between the chamber and the through hole.

[0014] In addition, according to one embodiment of the present invention, a sealing filter may be further included that is arranged on one side of the hole, and the sealing filter may be provided so as to allow gas inside the housing to pass through but not liquid inside the housing to pass through.

[0015] In addition, according to one embodiment of the present invention, when a sample introduced into the chamber through the delivery channel is greater than a set amount, the sample moves through the sealing channel, and the bottom surface of the sealing channel is formed above the bottom surface of the chamber, and the internal space of each chamber defined from the bottom surface of each chamber to the bottom surface of the sealing channel can have the same volume.

[0016] In addition, according to one embodiment of the present invention, the lower case includes a mounting portion that is formed inwardly from the lower case and has the through hole formed on one surface, and the sealing filter can be mounted on the mounting portion.

[0017] Additionally, according to one embodiment of the present invention, the distance through the delivery path from the sample injection unit to each chamber can be formed to be the same.

[0018] Additionally, according to one embodiment of the present invention, the transmission path extends while being embedded at a certain depth on the substrate, and at least a portion of the transmission path can form a bend between the sample injection portion and the chamber.

[0019] Additionally, according to one embodiment of the present invention, the lower case may include a heat transfer region formed on one side of the plurality of chambers and transferring heat from the outside of the lower case to the inside of the chamber.

[0020] In addition, according to one embodiment of the present invention, a waterproof film may be further included between the upper case and the substrate, and the sample injection unit may be provided to communicate with the injection opening formed in the upper case when a sample is injected through the injection opening while being blocked by the waterproof film.

[0021] Additionally, according to one embodiment of the present invention, the upper case may include a plurality of measuring openings formed at positions corresponding to the plurality of chambers.

[0022] Additionally, according to one embodiment of the present invention, two or more measuring openings may be formed at positions corresponding to one of the chambers.

[0023] The above-described problem-solving methods are merely exemplary and should not be construed as limiting the present invention. In addition to the exemplary embodiments described above, additional embodiments may be included in the drawings and detailed description of the invention.

[0024] According to the above-described means for solving the problem of the present invention, the present invention has the effect of providing a multi-channel molecular diagnostic cartridge that enables performing multiple types of molecular diagnostics with a single sample deployment.

[0025] In addition, the hospital can provide a multi-channel molecular diagnostic cartridge that can apply a network-based healthcare information sharing system when connected to a smart device.

[0026] In addition, the hospital has the effect of providing a multi-channel molecular diagnostic cartridge that enables rapid diagnosis of infectious diseases with similar symptoms but different prescriptions, enabling rapid and accurate diagnosis in the event of a large-scale infectious disease outbreak.

[0027] However, the effects achieved by the embodiments of the present invention are not limited to the technical tasks described above, and other effects may exist.

[0028] Figure 1 is a perspective view illustrating a multi-channel molecular diagnostic cartridge according to one embodiment of the present invention.

[0029] Figure 2 is a schematic exploded perspective view of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention.

[0030] Figure 3 is a cross-sectional view of a portion of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention.

[0031] FIG. 4 is a drawing showing a lower case (13) and a substrate (20) formed inside the lower case (13) according to one embodiment of the present invention.

[0032] Figure 5 is a drawing of the lower case (13) viewed from the lower side (bottom).

[0033] Figure 6 is a schematic cross-sectional view of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention.

[0034] Fig. 7 is a drawing illustrating a case in which a sample inside a chamber (23) reacts according to one embodiment of the present invention.

[0035] Fig. 8 is a drawing illustrating a case in which a sample inside a chamber (23) reacts according to another embodiment of the present invention.

[0036] Below, with reference to the attached drawings, embodiments of the present invention are described in detail to facilitate easy implementation by those skilled in the art. However, the present invention can be implemented in various different forms and is not limited to the embodiments described herein. In the drawings, irrelevant parts have been omitted for clarity, and similar reference numerals have been used throughout the specification to indicate similar elements.

[0037] Throughout this specification, when a part is said to be "connected" to another part, this includes not only "directly connected" but also "indirectly connected" with another component in between or "electrically connected" with a component in between.

[0038] Throughout this specification, when it is said that a member is located “on,” “above,” “upper,” “lower,” “lower” or “lower” another member, this includes not only cases where the member is in contact with the other member, but also cases where another member exists between the two members.

[0039] Throughout this specification, whenever a part is said to "include" a component, this does not mean that it excludes other components, but rather that it may include other components, unless otherwise specifically stated.

[0040] Terms such as "first~", "second~" may be used to indicate the same or substantially the same configuration in a different order, and may be interpreted as a configuration that is substantially the same as a configuration that is not indicated as "first", "second", etc.

[0041] In addition, terms related to direction or location (upper side, upper surface, lower side, etc.) in the description of the embodiments of the present invention are set based on the arrangement state of each component shown in the drawing.

[0042] Hereinafter, a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention will be described. The multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention configures a plurality of independent chambers inside the cartridge so that various liquid-based molecular diagnostic tests can be performed with a single sample injection, and the sample reaches each chamber at the same time, or the size of the chamber (23) where the sample is reacted is fixed to a constant value so that the reaction result of the sample can be observed.

[0043] Fig. 1 is a schematic perspective view of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention, and Fig. 2 is a schematic exploded perspective view of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention. Referring to Figs. 1 and 2, the multi-channel molecular diagnostic cartridge (1) may include a housing (10), a substrate (20), and a waterproof film (30).

[0044] A housing (10) according to one embodiment of the present invention forms a substantial outer shape of a multi-channel molecular diagnostic cartridge (1), and may be formed to accommodate various components, such as a sample injection portion (21) and a chamber (23), formed on a substrate (20) described below, inside. A sample (which may be understood as a liquid sample) may be injected into the housing (10) and moved to each chamber (23). The housing (10) may include an upper case (11), a lower case (13), an injection seal (15), and a joining member (17).

[0045] The upper case (11) can be formed to cover the substrate (20) at the upper part of the housing (10), and the lower case (13) can be formed to support the substrate (20).

[0046] The upper case (11) and the lower case (13) are coupled to each other so that a sample can be injected into the inner space. The coupling between the upper case (11) and the lower case (13) may be a fitting coupling via a coupling member (17), but is not limited thereto, and various coupling methods such as a tapered coupling or a coupling using a fastening member such as a screw may be used.

[0047] Continuing with reference to FIGS. 1 and 2, an injection opening (111) through which a sample is injected and a measurement opening (113) formed at a position corresponding to a chamber (23) described later can be formed in the upper case (11).

[0048] A sample can be injected through the injection opening (111), and an injection seal (15) can be placed inside the injection opening (111) to prevent the sample from leaking between the upper case (11) and the lower case (13). In addition, a joint structure can be formed in the injection opening (111) portion to prevent the sample from leaking out through the gap of the joint surface between the upper case (11) and the lower case (13).

[0049] A plurality of measurement openings (113) are formed spaced apart from each other to face the chamber (23), and can be opened so that light is irradiated into the chamber (23) by an optical sensor of an external optical diagnostic device. Here, it is preferable that a plurality of measurement openings (113) are formed spaced apart from each other in the same direction according to the arrangement direction of the chamber (23), and light from the optical diagnostic device is irradiated toward the chamber (23) through the open space of the corresponding measurement openings (113), and the irradiated light is reflected and re-injected into the optical sensor of the optical diagnostic device to analyze the optical characteristics so that a diagnosis can be made. The correspondence between the measurement openings (113) and the chamber (23) will be described later.

[0050] Fig. 3 is a cross-sectional view of a portion of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention. Referring to Fig. 3, the upper case (11) can be divided into an injection portion (11a) and a reaction portion (11b). An injection opening (111) can be formed in the injection portion (11a), and a measurement opening (113) can be formed in the reaction portion (11b). That is, the injection portion (11a) can be understood as a portion into which a sample is injected through the injection opening, and the reaction portion (11b) can be understood as a portion provided so that the injected sample moves toward the chamber (23) to observe the reaction of the sample.

[0051] In one embodiment, the injection portion (11a) may be formed to have a higher height than the reaction portion (11b). When a sample is injected through the injection opening (111), if it is injected at excessive pressure and speed, the sample may overflow out of the housing (10). By forming the injection portion (11a) higher than the reaction portion (11b), leakage of the sample can be prevented. As illustrated in FIG. 3, when a sample is injected, the sample can be accommodated within the space formed by the sample injection portion (21) and the injection opening (111), so that the sample can remain in a larger space than when the sample remains only within the sample injection portion (21) in the lower case (13).

[0052] FIG. 4 is a drawing showing a lower case (13) and a substrate (20) formed inside the lower case (13) according to one embodiment of the present invention, and FIG. 5 is a drawing showing the lower case (13) as viewed from the lower side (bottom). Referring to FIGS. 2, 4, and 5, the substrate (20) is formed inside the lower case (13), and a sample injection portion (21), a delivery path (22), a chamber (23), etc. can be formed on the substrate.

[0053] A recessed portion (13a) recessed toward the substrate (20) can be formed on one side (which can be understood as the bottom) of the lower case (13).

[0054] In addition, the lower case (13) may include a mounting portion (131) that is formed by being recessed inward (on the substrate side) from the lower case and has a through hole (25) formed on one side thereof, which will be described later. A sealing filter (26) is mounted on the mounting portion (131), thereby allowing gas inside the housing (10) to pass through while preventing liquid from passing through, thereby preventing the sample from overflowing the chamber (23) and leaking.

[0055] In one embodiment, the lower case (13) is formed on one side of a plurality of chambers (23) and may further include a heat transfer area (133) that transfers heat from the outside of the lower case to the inside of the chamber (23). When the solution inside the chamber (23) needs to be reacted at a constant temperature, heat can be transferred through the heat transfer area (133).

[0056] In a preferred embodiment, a plurality of chambers (23) may be arranged with equal spacing on a substrate (20) located in the reaction portion (11b), and a heat transfer area (133) may be provided to extend along the arranged chambers (23) and transfer heat from the lower side of the chambers (23). A heating element may be brought into contact with the heat transfer area (133) to reach an appropriate temperature, or power or the like may be applied to the heat transfer area (133) to generate heat. For example, the lower case (13) may be formed of synthetic resin or plastic, but the heat transfer area (133) may be formed of a metal having better thermal conductivity than the lower case (13).

[0057] The substrate (20) is formed to protrude upward from the lower case (13) by a predetermined height, and it can be understood that a waterproof film (30) is interposed between the substrate (20) and the upper case (11). A sample introduced into the housing (10) through the injection opening (111) flows on the substrate and reacts within the chamber, thereby enabling molecular diagnosis to be performed.

[0058] A sample injection portion (21), a delivery path (22), a chamber (23), a sealing path (24), and a through hole (25) can be formed on the substrate (20), and a sealing filter (26) can be placed on one side of the through hole (25).

[0059] The sample injection part (21) is formed by being inserted into the substrate of the lower case, and can receive a sample through an injection opening formed in the upper case.

[0060] The delivery path (22) may extend between the sample injection unit (21) and a plurality of independent chambers (23), and each delivery path (22) may be formed to correspond one-to-one with a chamber (23) so as to move the sample in the sample injection unit to the corresponding chamber (23). That is, the number of delivery paths (22) may be equal to the number of chambers (23). In addition, the delivery path (22) may extend while being embedded at a certain depth on the substrate (20).

[0061] In one embodiment, the distance through the delivery path (22) from the sample injection unit (21) to each chamber (23) can be formed to be the same. Through this, when a sample is injected into the sample injection unit (21), the sample can be delivered to each chamber (23) at the same time.

[0062] To this end, at least some of the delivery channels (22) may form bends between the sample injection unit (21) and the chambers (23). Since a plurality of chambers (23) are independently formed and spaced apart from each other, the straight-line distance from the sample injection unit (21) to each chamber (23) is different. Accordingly, by forming bends in the delivery channels (22), the sample can be equally delivered from the sample injection unit (21) to the chambers (23). Here, it can be understood that the delivery channels (22) connecting chambers (23) having a short straight-line distance from the sample injection unit (21) have fewer bends, and the delivery channels (22) connecting chambers (23) having a long straight-line distance have many bends.

[0063] The chamber (23) can be formed by inserting a sample into the substrate of the lower case, and a sample can be introduced into the chamber (23) through the delivery path (22) to perform molecular diagnosis through a reaction.

[0064] The color-developed sample reacted within each chamber (23) can be measured through the measurement opening (113). For example, each of the five chambers (23) is individual and distinct from each other, and can independently receive samples by being connected to the sample injection unit (21) through different delivery channels (22). Different reactants, for example, molecular diagnostic substances for samples, are provided within each chamber (23), and different reaction results can be produced depending on the disease to be diagnosed for the sample.

[0065] Each chamber (23) can be formed independently within the substrate (20). It can be understood that a partition structure is installed between each chamber (23), and is formed so that the sample cannot pass over to the chamber (23) right next to it. When the sample within the chamber (23) rises to a certain height, the liquid sample moves through the sealing passage (24), and leakage can be prevented by the sealing filter (26) provided in the hole (25). The air within the chamber (23) and the sealing passage (24) can be discharged outside the housing (10) by the hole (25) and the sealing filter (26), while the sample is prevented from leaking out of the housing, thereby preventing an excessive amount of sample from being transferred within each chamber (23) and allowing the same amount of sample to be transferred.

[0066] The sealing urea (24) can be connected to the chamber (23) at one end and to the through hole (25) at the other end. That is, one end of the chamber (23) can be connected to the transmission urea (22) and the other end can be connected to the sealing urea. Accordingly, the sealing urea (24)

[0067] The through hole (25) can extend between the upper surface of the water channel substrate (20) corresponding to the chamber and one surface of the lower case (13). The sealing passage (24) inside the housing and one side (which can be understood as the outside or lower side) of the lower case (13), i.e. the outside of the housing, can be connected by the through hole (25).

[0068] The sealing filter (26) may be arranged on one side of the aperture (25), preferably on the lower side or the outer side. The sealing filter (26) may be provided so as to allow gas inside the housing to pass through, but not allow liquid inside the housing to pass through. In one embodiment, the sealing filter (26) may be formed to have a plurality of pores, and may be provided so as to allow gas to pass through when not in contact with a sample, but may be provided so as to close the aperture (25) by blocking the pores by the sample when in contact with the sample.

[0069] Accordingly, as described below, when a sample is introduced, the gas in the chamber (23) and the sealing passage (24) can be discharged to the outside of the housing through the opening (25) and the sealing filter (26) according to the introduction of the sample. In addition, when an excessive amount of sample is introduced, the sample fills the chamber (23) and moves through the sealing passage (24), and the sealing filter (26) can block the opening (25) due to the sample, thereby preventing any more sample from being injected into the chamber (23).

[0070] Fig. 6 is a schematic cross-sectional view of a multi-channel molecular diagnostic cartridge (1) according to one embodiment of the present invention. Referring to Fig. 6, a sample is introduced into a chamber (23) through a delivery path (22) from a sample injection unit (21), and when the chamber is filled, the sample can move through a sealing path (24). Thereafter, the sample is directed toward a hole (25) at the end side of the sealing path (24), so that a sealing filter (26) can close the hole (25) by the sample. Meanwhile, the sealing filter (26) is seated inside a mounting portion (131) formed on the outside of the housing (10), so that replacement of the sealing filter (26) can be easily performed.

[0071] As illustrated, the depth at which the delivery path (22) and the sealing path (24) are embedded from the substrate (20) may be different from the depth at which the chamber (23) is embedded from the substrate. That is, when a sample introduced into the chamber (23) through the delivery path (22) exceeds a set amount, the bottom surface of the sealing path (24) may be formed above the bottom surface of the chamber (23).

[0072] The internal space of the chamber (23) in which the sample is accommodated can be defined as the area from the bottom surface of the chamber (23) to the bottom surface (h) of the sealing passage (24). That is, the sample introduced into the chamber (23) through the delivery passage (22) fills the internal space of the chamber and then flows through the sealing passage (24) when a set volume or a set amount or more is introduced.

[0073] In one embodiment, the internal spaces of the plurality of chambers (23) may be formed to have the same volume. Accordingly, the sample injected into the sample injection unit (21) can be uniformly delivered to each chamber (23) to perform a reaction for molecular diagnosis.

[0074] In an embodiment not shown, a partition wall is formed between the sealing passage (24) and the chamber (23), and when the chamber (23) is filled with a sample exceeding a set amount, the sample may flow over the partition wall into the sealing passage (24). At this time, the height of the partition wall may be formed to be lower than the height from the bottom surface of the chamber (23) to the top surface of the substrate (20). In this case, the bottom surface of the sealing passage (24) and the bottom surface of the chamber (23) may be the same height, or may have different heights.

[0075] Referring again to FIGS. 2 and 3, the waterproof film (30) can be attached to the lower case (13), preferably the substrate (20), in a colorless, transparent, and thin shape, and when a reagent is injected into the sample injection unit (21), when a sample moves along the delivery path (22), it can prevent leakage onto the surface during the process of the sample reaching the chamber (23) through each delivery path (22). The waterproof film (30) can form a through hole in the portion where the upper case (11) and the lower case (13) are joined by the joining member (17) to allow the joining member (17) to penetrate therethrough.

[0076] The sample is injected into the sample injection port (21) through the injection opening (111). The space between the sample injection port (21) and the injection opening (111) is blocked with a waterproof film (3) to prevent leakage, and the sample can be injected by penetrating the waterproof film (30). The sample injected into the sample injection port (21) travels through the delivery path (22) to each independent chamber (23) and causes a reaction. The color development of the sample in the chamber (23) can be measured through the measurement opening (113).

[0077] FIG. 7 is a diagram illustrating a case where a sample inside a chamber (23) reacts according to one embodiment of the present invention. Referring to FIG. 7, a measurement opening (113) may be formed at a position corresponding to a plurality of chambers (23). Here, the corresponding position may be understood as being formed at a position where light is irradiated into the chamber (23) by an optical sensor of an external optical diagnostic device. In one embodiment, the measurement opening (113) may be formed to correspond one-to-one with the chamber (23). The concentration of the sample that has developed color after the reaction inside the chamber (23) is measured by an optical sensor, and the negative or positive result of the test in multiple chambers (23) can be confirmed at once based on the measured value.

[0078] Fig. 8 is a diagram illustrating a case where a sample inside a chamber (23) reacts according to another embodiment of the present invention. Two or more measurement openings (113) may be formed at positions corresponding to one chamber (23). Different light may be irradiated to each measurement opening (113) from an optical sensor, or light may be irradiated to only one of the measurement openings (113) to confirm the reaction results inside the chamber (23).

[0079] The above description of the present invention is for illustrative purposes only, and those skilled in the art will readily appreciate that the present invention can be readily modified into other specific forms without altering the technical spirit or essential characteristics of the present invention. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. For example, each component described as a single entity may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined manner.

[0080] The scope of the present invention is indicated by the claims described below rather than the detailed description above, and all changes or modifications derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present invention.

[0081] [Explanation of symbols]

[0082] 1: Multichannel molecular diagnostic cartridge

[0083] 10: Housing

[0084] 11: Upper case

[0085] 13: Lower case

[0086] 15: Injection sealing

[0087] 20: Substrate

[0088] 21: Sample injection unit

[0089] 22: Euro transfer

[0090] 23: Chamber

[0091] 24: Schilling Euro

[0092] 25: Tonggong

[0093] 26: Sealing filter

[0094] 30: Waterproof film

Claims

1. A housing having an upper case and a lower case; A sample injection unit formed by insertion from the substrate of the lower case and receiving a sample through an injection opening formed in the upper case; A plurality of chambers formed by insertion from the substrate of the lower case; and Including a plurality of transfer channels extending between the sample injection portion and the plurality of chambers, A multi-channel molecular diagnostic cartridge, wherein each of the above delivery channels corresponds one-to-one with each chamber connected to the above delivery channel and is provided to move a sample in the sample injection unit to the corresponding chamber.

2. In paragraph 1, A through hole extending between the upper surface of the substrate and one surface of the lower case corresponding to the chamber; and A multichannel molecular diagnostic cartridge further comprising a sealing channel extending between the chamber and the opening.

3. In paragraph 2, Further comprising a sealing filter arranged on one side of the above-mentioned opening, A multi-channel molecular diagnostic cartridge, wherein the sealing filter is provided to allow gas inside the housing to pass through but not liquid inside the housing to pass through.

4. In paragraph 3, When the sample introduced into the chamber through the above-mentioned transmission path exceeds a set amount, the sample moves through the above-mentioned sealing path, and the bottom surface of the above-mentioned sealing path is formed above the bottom surface of the chamber. A multi-channel molecular diagnostic cartridge, wherein the internal space of each chamber, defined from the bottom surface of each chamber to the bottom surface of the sealing channel, has the same volume.

5. In paragraph 3, The above lower case includes a mounting portion that is formed inwardly from the lower case and has the above hole formed on one surface, A multi-channel molecular diagnostic cartridge, wherein the sealing filter is mounted on the above-mentioned mounting portion.

6. In paragraph 1, A multi-channel molecular diagnostic cartridge formed such that the distance through the delivery path from the sample injection unit to each chamber is the same.

7. In paragraph 6, The above-mentioned transmission path extends while being embedded at a certain depth on the above-mentioned substrate, A multichannel molecular diagnostic cartridge, wherein at least some of the above-described transmission channels form a bend between the sample injection portion and the chamber.

8. In paragraph 1, The above sub-case is, A multi-channel molecular diagnostic cartridge, comprising a heat transfer region formed on one side of a plurality of the chambers and transferring heat from the outside of the lower case to the inside of the chamber.

9. In paragraph 1, Further comprising a waterproof film disposed between the upper case and the substrate, A multi-channel molecular diagnostic cartridge, wherein the sample injection portion is provided to communicate with the injection opening formed in the upper case when a sample is injected through the injection opening while the sample is blocked by the waterproof film.

10. In paragraph 1, A multi-channel molecular diagnostic cartridge, wherein the upper case includes a plurality of measuring openings formed at positions corresponding to the plurality of chambers.

11. In paragraph 10, A multi-channel molecular diagnostic cartridge, wherein two or more measuring openings are formed at positions corresponding to one of the chambers.

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