Sample analyzer

Abstract

The present application is suitable for the field of in-vitro diagnosis apparatuses. Provided is a sample analyzer, the sample analyzer comprising a sample distribution device, a nucleic acid extraction device, a pipetting device, an amplification device and a test device. The nucleic acid extraction device comprises a first functional assembly, a second functional assembly and a transfer assembly, the first functional assembly being provided above or below the second functional assembly, the transfer assembly being used for transferring a nucleic acid extraction container between the first functional assembly and the second functional assembly, the first functional assembly being used for at least performing magnetic attraction and liquid suction actions on a liquid in the nucleic acid extraction container, and the second functional assembly being at least used for incubating the liquid in the nucleic acid extraction container. The first functional assembly and the second functional assembly which are used for bearing the nucleic acid extraction container to perform the functional actions in the nucleic acid extraction device being arranged up and down can reduce the space occupied by the nucleic acid extraction device in the horizontal direction, thereby facilitating miniaturization of the sample analyzer.

Description

Sample analyzer

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application is based on the Chinese patent application with application number 2023118683007 and application date of December 29, 2023; and the Chinese patent application with application number 2024105383920 and application date of April 29, 2024, and claims the priority of these two Chinese patent applications. The entire contents of these two Chinese patent applications are hereby introduced into this application as a reference. Technical Field

[0003] The present invention relates to the field of in vitro diagnostic equipment, and in particular to a sample analyzer. Background Art

[0004] Molecular diagnostic technology uses DNA and RNA as diagnostic materials and molecular biology techniques to diagnose human conditions and diseases by detecting the presence, defects, or abnormal expression of genes. Its basic principle is to detect changes in the structure, quantity, and function of DNA or RNA to determine whether the subject has abnormal genetic changes. This is of great significance for the prevention, prediction, diagnosis, treatment, and prognosis of diseases. Molecular diagnostic all-in-one devices are testing equipment that perform molecular diagnostic tests. Because molecular diagnostics require multiple steps, such as sample preparation, nucleic acid extraction, and reaction system setup, the corresponding all-in-one molecular diagnostic devices contain numerous functional modules. To ensure a complete sample input and result output, numerous auxiliary modules are also required, such as reagent management, waste management, and contamination prevention. The integration of such numerous modules often results in a very large all-in-one device. The molecular laboratories where molecular diagnostic all-in-one devices are placed must be partitioned and isolated to prevent contamination, and maintain a negative pressure laboratory environment. Consequently, molecular laboratories are generally small, and space is at a premium. Placing a large all-in-one device in a molecular laboratory presents a significant challenge for users. For manufacturers of molecular all-in-one machines, it is also imperative to reduce the size of the machine.

[0005] In a molecular diagnostic all-in-one device, the multiple stations of the nucleic acid extraction device are arranged horizontally in a production-line fashion. This results in a very large volume and occupied horizontal space for the nucleic acid extraction device, seriously hindering the miniaturization of the molecular diagnostic all-in-one device. The nucleic acid extraction device is one of the functional modules that occupies the largest space in the molecular diagnostic all-in-one device. Optimizing its design and structural layout is crucial to the compactness of the entire molecular diagnostic all-in-one device. Summary of the Invention

[0006] A first object of the present invention is to provide a sample analyzer, which aims to solve the technical problem in the related art that the nucleic acid extraction device occupies a large horizontal space.

[0007] To achieve the above object, the present invention provides the following solutions:

[0008] A sample analyzer, comprising:

[0009] A sample dispensing device, the sample dispensing device being used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container;

[0010] a nucleic acid extraction device for extracting nucleic acid from a liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract, wherein the reagent contains magnetic beads;

[0011] a pipetting device, used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0012] an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0013] A detection device, the detection device is used to detect the liquid to be tested;

[0014] In which, the nucleic acid extraction device includes a first functional component, a second functional component and a transfer component, the first functional component is arranged above or below the second functional component, and the transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component to perform the nucleic acid extraction to obtain the nucleic acid extract; the first functional component is used to at least perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container, and the second functional component is at least used to incubate the liquid in the nucleic acid extraction container.

[0015] As an embodiment, the first functional component includes a first carrying member, a first pipetting member and a first magnetic attraction member, the first carrying member is used to carry the nucleic acid extraction container, the first magnetic attraction member is used to perform a first magnetic adsorption on the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the first carrying member to adsorb the magnetic beads in the nucleic acid extraction container, the first pipetting member is used to perform a first liquid aspiration action on the liquid in the nucleic acid extraction container located on the first carrying member and subjected to the first magnetic adsorption by the first magnetic attraction member, the transfer component is used to transfer the nucleic acid extraction container between the first carrying member and the second functional component, the magnetic adsorption includes the first magnetic adsorption, and the liquid aspiration action includes the first liquid aspiration action.

[0016] As an embodiment, the first functional component further includes a liquid injection component, and the liquid injection component is used to perform a first liquid injection action of injecting a cleaning liquid into the nucleic acid extraction container located on the first supporting component.

[0017] As an embodiment, the first functional component also includes a second carrying member, a second pipetting member and a second magnetic attraction member, the second carrying member is used to carry the nucleic acid extraction container, the second magnetic attraction member is used to perform a second magnetic adsorption on the liquid in the nucleic acid extraction container located on the second carrying member that at least contains a cleaning liquid, and the second pipetting member is used to perform a second liquid aspiration action on the liquid in the nucleic acid extraction container located on the second carrying member and subjected to the second magnetic adsorption by the second magnetic attraction member; the transfer component is also used to transfer the nucleic acid extraction container between the second carrying member and the second functional component, the magnetic adsorption also includes the second magnetic adsorption, and the liquid aspiration action also includes the second liquid aspiration action.

[0018] As an embodiment, the first functional component also includes a third carrying member and a third pipetting member, the third carrying member is used to carry the nucleic acid extraction container, and the third pipetting member is used to perform a second liquid injection action of injecting elution reagent into the nucleic acid extraction container located on the third carrying member; the transfer component is also used to transfer the nucleic acid extraction container between the third carrying member and the second functional component.

[0019] As an embodiment, the sample analyzer further includes a first lifting member; the first lifting member is connected to the second magnetic member, and the first lifting member is used to drive the second magnetic member to move up and down relative to the nucleic acid extraction container.

[0020] In one embodiment, the nucleic acid extraction container is formed with a reaction well, a first pipette tip hole, and a second pipette tip hole. The reaction well is used to carry samples and reagents for nucleic acid extraction. The first pipette tip hole is used to carry a first pipette tip. The second pipette tip hole is used to carry a second pipette tip. The volume of the first pipette tip is greater than that of the second pipette tip.

[0021] The first pipetting member includes a first pipette tip connecting component and a first suction and discharge power component; the first pipette tip connecting component is used to be detachably connected to the first pipette tip so as to aspirate liquid through the first pipette tip under the action of power provided by the first suction and discharge power component;

[0022] The third pipetting component includes a second pipette head connecting component and a second suction and discharge power component. The second pipette head connecting component is used to be detachably connected to the second pipette head so as to aspirate liquid through the second pipette head under the power provided by the second suction and discharge power component.

[0023] As an embodiment, the nucleic acid extraction container is further formed with a waste liquid hole, and the first pipetting member and the second pipetting member are further used to discharge the liquid sucked from the reaction hole into the waste liquid hole.

[0024] As an embodiment, the number of the first carrying members is at least two, and each of the first carrying members is correspondingly provided with at least one liquid injection member and at least one first liquid transfer member.

[0025] As an embodiment, the first bearing member, the second bearing member and the third bearing member are arranged side by side in a horizontal direction.

[0026] As an embodiment, the second functional component includes a fourth supporting member and a heating member, the fourth supporting member is used to support the nucleic acid extraction container to perform an incubation action, and the heating member is used to heat the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth supporting member; the fourth supporting member includes a lysis zone, a washing zone, a drying zone and an elution zone, and the lysis zone, the washing zone, the drying zone and the elution zone are respectively formed with a receiving cavity for placing the nucleic acid extraction container, and the transfer component is used to transfer the nucleic acid extraction container between the lysis zone, the washing zone and the first supporting member, between the washing zone, the drying zone and the second supporting member, and between the drying zone, the elution zone and the third supporting member.

[0027] As an embodiment, the fourth supporting member includes an incubation tray, and the heating member is used to heat the nucleic acid extraction container on the incubation tray; the incubation tray is formed with the lysis zone, the washing zone, the drying zone and the elution zone, and a first transfer station is formed on the incubation tray, and the incubation tray is used to dispatch the nucleic acid extraction containers located in the lysis zone, the washing zone, the drying zone and the elution zone to the first transfer station respectively, and the transfer component is used to dispatch the nucleic acid extraction container located at the first transfer station to the first functional component.

[0028] As an embodiment, the sample analyzer further includes a controller, and the controller is configured to:

[0029] controlling the transfer component to dispatch the nucleic acid extraction container after performing the incubation action in the lysis zone to the first carrying member;

[0030] controlling the first pipetting member to perform the first liquid aspirating action on the liquid in the nucleic acid extraction container in the first carrying member and controlling the liquid injection member to perform the first liquid injection action on the nucleic acid extraction container in the first carrying member;

[0031] Controlling the transfer component to transport the nucleic acid extraction container after performing the first liquid injection action to the washing area to perform the incubation action;

[0032] Controlling the transfer component to transfer the nucleic acid extraction container after performing the incubation action in the washing area to the second carrying member;

[0033] controlling the second pipetting member to perform the second liquid aspirating action on the liquid in the nucleic acid extraction container in the second carrying member;

[0034] Controlling the transfer component to transport the nucleic acid extraction container after the second liquid aspiration operation to the drying area to perform a drying operation on the nucleic acid extraction container;

[0035] controlling the transfer assembly to transfer the nucleic acid extraction container after the drying operation is performed in the drying area to the third carrying member;

[0036] controlling the third pipetting member to perform the second liquid injection action on the nucleic acid extraction container in the third carrying member;

[0037] The transfer component is controlled to transport the nucleic acid extraction container after performing the second liquid injection action to the elution area to perform the elution action.

[0038] In one embodiment, the position of the bottom of the accommodating chamber in the elution zone is higher than the position of the bottom of the accommodating chamber in the lysis zone, higher than the position of the bottom of the accommodating chamber in the washing zone, and higher than the position of the bottom of the accommodating chamber in the drying zone; and / or,

[0039] The bottom of the accommodating chamber in the cracking zone is at the same height as the bottom of the accommodating chamber in the washing zone and the bottom of the accommodating chamber in the drying zone.

[0040] As an embodiment, the second functional component includes a fourth supporting member, a heating member and a mixing member. The fourth supporting member is used to support the nucleic acid extraction container to perform an incubation action. The heating member is used to heat the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth supporting member; the mixing member is used to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth supporting member.

[0041] As an embodiment, the second functional component further includes a first driving member, the first driving member being configured to drive the fourth bearing member to move so that the fourth bearing member drives the nucleic acid extraction container to move;

[0042] The mixing component includes a plurality of magnets, and the plurality of magnets are alternately distributed on opposite sides of the movement track of the nucleic acid extraction container in the horizontal direction.

[0043] As an embodiment, the mixing member comprises at least a first magnet array and a second magnet array extending horizontally, and each of the magnet arrays comprises a plurality of magnets installed at intervals on a magnet bracket;

[0044] The fourth supporting member includes a container receiving portion for placing the nucleic acid extraction container;

[0045] The fourth carrying member and the mixing member are arranged relative to each other so that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array;

[0046] The fourth supporting member and the mixing member are capable of horizontally moving relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion;

[0047] The fourth supporting member and the mixing member are further arranged relative to each other so that when the fourth supporting member and the mixing member move horizontally relative to each other, at least two magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

[0048] As an embodiment, the at least two magnets in the mixing member have different heights relative to the fourth bearing member; or,

[0049] The at least two magnets in the mixing member are installed at different heights on the magnet bracket, so that the at least two magnets in the mixing member have different heights relative to the fourth supporting member.

[0050] As an embodiment, the second functional component further includes a third driving member, the mixing member includes a movable magnet holder and a plurality of magnets fixed on the magnet holder, wherein the third driving member is used to drive the magnet holder to move, so as to drive the plurality of magnets fixed on the magnet holder to move relative to the nucleic acid extraction container on the fourth supporting member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth supporting member perform three-dimensional movement in the liquid in the nucleic acid extraction container under the action of the moving magnet; or,

[0051] The second functional component further includes a third driving member, the mixing member is used to generate a magnetic field, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, thereby enabling the magnetic beads in the nucleic acid extraction container to perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or,

[0052] The second functional component also includes a third driving member, and the fourth bearing member includes at least a lysis zone. The nucleic acid extraction container undergoes a lysis and capture link in the lysis zone. In this lysis and capture link, cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field that at least covers the lysis zone. The third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis zone move so that the magnetic beads capture the released nucleic acids.

[0053] As an embodiment, the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is mounted on the frame assembly;

[0054] The second functional component includes a fourth bearing member and a first driving member. The fourth bearing member can be movably mounted on the frame assembly to carry the nucleic acid extraction container to perform incubation, and the fourth bearing member is formed with at least one first transfer position and multiple accommodating cavities, each of the accommodating cavities is used to accommodate one nucleic acid extraction container, the first driving member is used to drive the fourth bearing member to move relative to the frame assembly so that the fourth bearing member drives the multiple nucleic acid extraction containers to move to the first transfer position respectively; the first bearing member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer position and the first bearing member.

[0055] As an embodiment, the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is mounted on the frame assembly;

[0056] The second functional component includes a fourth bearing member, which is fixed on the frame assembly to carry the nucleic acid extraction container to perform incubation, and the fourth bearing member is formed with at least one first transfer station and multiple accommodating cavities, each of which is used to accommodate one nucleic acid extraction container; the first bearing member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer station and the first bearing member, and the transfer assembly is configured to perform at least three-dimensional movement.

[0057] As an embodiment, the sample analyzer further includes:

[0058] A container providing device, the container providing device is used to provide the nucleic acid extraction container and the amplification reaction container;

[0059] a reagent storage compartment, the reagent storage compartment being used to store a first type of reagent and a second type of reagent, the first type of reagent being used to react with a sample to obtain a nucleic acid extract, and the second type of reagent being used to react with the nucleic acid extract to perform amplification;

[0060] a reagent dispensing device for dispensing the first type of reagent stored in the reagent storage compartment into the nucleic acid extraction container, and for dispensing the second type of reagent stored in the reagent storage compartment into the amplification reaction container;

[0061] A scheduling device is used at least to schedule the nucleic acid extraction container provided by the container providing device to the nucleic acid extraction device and to schedule the amplification reaction container provided by the container providing device to the amplification device.

[0062] A second object of the present invention is to provide a sample analyzer, comprising:

[0063] A sample dispensing device, the sample dispensing device being used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container;

[0064] a nucleic acid extraction device for extracting nucleic acid from the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract;

[0065] a pipetting device, used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0066] an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0067] A detection device, the detection device is used to detect the liquid to be tested;

[0068] The nucleic acid extraction device includes a first functional component, a second functional component, and a transfer component. The first functional component is arranged above or below the second functional component. The transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component. The first functional component is at least used to carry the nucleic acid extraction container to perform a first functional action, and the second functional component is at least used to carry the nucleic acid extraction container to perform a second functional action to perform nucleic acid extraction.

[0069] The first functional action and the second functional action are different functional actions or the same functional action executed successively.

[0070] As an embodiment, the first functional action includes at least one of a liquid suction action, a liquid injection action, and a magnetic adsorption action; and / or,

[0071] The second functional action includes at least one of an incubation action and a mixing action.

[0072] In one embodiment, the second functional component includes a fourth carrying member and a mixing member, wherein the fourth carrying member is used to carry the nucleic acid extraction container to perform an incubation action, and the mixing member is used to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container located on the fourth carrying member;

[0073] Wherein, the mixing component comprises at least a first magnet array and a second magnet array extending horizontally, and each of the magnet arrays comprises a plurality of magnets installed at intervals on a magnet bracket;

[0074] The fourth supporting member includes a container receiving portion for placing the nucleic acid extraction container;

[0075] The fourth carrying member and the mixing member are arranged relative to each other so that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array;

[0076] The fourth supporting member and the mixing member are capable of horizontally moving relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion;

[0077] The fourth supporting member and the mixing member are further arranged relative to each other so that when the fourth supporting member and the mixing member move horizontally relative to each other, at least two magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

[0078] As an embodiment, the second functional component includes a fourth bearing member, a mixing member and a third driving member, the fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action, the mixing member is used to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth bearing member, the mixing member includes a movable magnet bracket and a plurality of magnets fixed on the magnet bracket, the third driving member is used to drive the magnet bracket to move, so as to drive the plurality of magnets fixed on the magnet bracket to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement in the liquid in the nucleic acid extraction container under the action of the moving magnet; or,

[0079] The second functional component includes a fourth bearing member, a mixing member and a third driving member, the fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action, the mixing member is used to generate a magnetic field to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth bearing member, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, thereby enabling the magnetic beads in the nucleic acid extraction container to perform three-dimensional movement in the liquid in the nucleic acid extraction container under the action of the alternating magnetic field; or,

[0080] The second functional component includes a fourth bearing member, a mixing member and a third driving member. The fourth bearing member is used to carry the nucleic acid extraction container to perform an incubation action. The fourth bearing member includes at least a lysis zone. The nucleic acid extraction container undergoes a lysis and capture link in the lysis zone. In this lysis and capture link, cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field that at least covers the lysis zone to at least mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container located in the lysis zone. The third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis zone move so that the magnetic beads capture the released nucleic acids.

[0081] A third object of the present invention is to provide a sample analyzer, comprising:

[0082] A sample dispensing device, the sample dispensing device being used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container;

[0083] a nucleic acid extraction device for extracting nucleic acid from the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract;

[0084] a pipetting device, used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0085] an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0086] A detection device, the detection device is used to detect the liquid to be tested;

[0087] Among them, the nucleic acid extraction device has at least two workstations, each of which is used to carry the nucleic acid extraction container to perform a processing action to extract nucleic acid. At least two of the workstations are distributed up and down in the vertical direction, and the processing actions include at least one of incubation action, mixing action, magnetic adsorption action, liquid aspiration action, and liquid injection action.

[0088] As an embodiment, the nucleic acid extraction device extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least the sample and the reagent and performing post-lysis processing.

[0089] As an embodiment, the nucleic acid extraction device has a first space and a second space, and the first space is arranged above or below the second space;

[0090] The first layer of space is provided with a liquid suction and injection station, a single liquid suction station and a single liquid injection station;

[0091] The second layer of space is provided with a lysis incubation station, a cleaning incubation station, a drying incubation station and an elution incubation station;

[0092] Wherein, the lysis and incubation station is used to carry the nucleic acid extraction container to perform lysis and incubation actions;

[0093] The cleaning and incubation station is used to carry the nucleic acid extraction container to perform cleaning and incubation actions;

[0094] The drying and incubation station is used to carry the nucleic acid extraction container to perform drying and incubation actions;

[0095] The elution and incubation station is used to carry the nucleic acid extraction container to perform elution and incubation actions;

[0096] The aspiration and injection station is used to carry the nucleic acid extraction container to perform a first aspiration action and a first injection action of injecting a cleaning solution;

[0097] The single liquid aspiration station is used to carry the nucleic acid extraction container to perform a second liquid aspiration action;

[0098] The single liquid injection station is used to carry the nucleic acid extraction container to perform a second liquid injection action of injecting an elution reagent.

[0099] A fourth object of the present invention is to provide a sample analyzer, comprising:

[0100] A sample dispensing device, the sample dispensing device being used to draw a sample from a sample container and dispense at least a portion of the drawn sample into a nucleic acid extraction container;

[0101] a nucleic acid extraction device for performing a nucleic acid extraction operation on the liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract;

[0102] a pipetting device, used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container;

[0103] an amplification device, the amplification device being used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution;

[0104] A detection device, the detection device is used to detect the liquid to be tested;

[0105] In which, the nucleic acid extraction device includes a lysis module, a cleaning module, an elution module and a transfer assembly. At least two of the lysis module, the cleaning module and the elution module are distributed in the vertical direction. The lysis module is used to carry the nucleic acid extraction container for lysis operation; the cleaning module is used to carry the nucleic acid extraction container for cleaning operation; the elution module is used to carry the nucleic acid extraction container for elution operation; the transfer assembly is used to transfer the nucleic acid extraction container to the lysis module, the cleaning module and the elution module in sequence.

[0106] As an embodiment, the nucleic acid extraction device extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least the sample and the reagent and performing post-lysis processing.

[0107] As an embodiment, the nucleic acid extraction device also includes a drying module, which is arranged below or above at least one of the lysis module, the cleaning module, and the elution module. The drying module is used to carry the nucleic acid extraction container for drying operations, and the transfer component is used to transfer the nucleic acid extraction container to the lysis module, the cleaning module, the drying module, and the elution module in sequence.

[0108] The first object of the present invention provides a sample analyzer, which is configured such that a first functional component in a nucleic acid extraction device for at least performing magnetic adsorption and liquid aspiration on a liquid in a nucleic acid extraction container is arranged above or below a second functional component for at least incubating the liquid in the nucleic acid extraction container, and the nucleic acid extraction container is transferred between the first functional component and the second functional component by a transfer component. That is, the first functional component and the second functional component for performing different functional actions on the liquid in the nucleic acid extraction container are arranged vertically in an upper and lower direction. When the liquid in the nucleic acid extraction container needs to perform an incubation action, the transfer component transfers the nucleic acid extraction container to the second functional component; when the liquid in the nucleic acid extraction container needs to perform magnetic adsorption and liquid aspiration actions, the transfer component transfers the nucleic acid extraction container to the first functional component. This arrangement scheme can fully utilize the vertical space to arrange the different functional components of the nucleic acid extraction device, thereby reducing the horizontal space occupied by the nucleic acid extraction device, and further facilitating the miniaturized design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located.

[0109] The sample analyzer provided by the second purpose of the present invention is configured such that one of the first functional component and the second functional component for performing different functional actions or for performing the same functional action successively in the nucleic acid extraction device is arranged above or below the other, and the nucleic acid extraction container is transferred between the first functional component and the second functional component through a transfer component, that is, the first functional component and the second functional component for performing different functional actions on the liquid in the nucleic acid extraction container or for performing the same functional action successively are arranged to be arranged upper and lower in the vertical direction, and the nucleic acid extraction container is transferred to the first functional component and the second functional component respectively through the transfer component. This arrangement scheme can make full use of the vertical space to arrange the different functional components of the nucleic acid extraction device, thereby reducing the horizontal occupied space of the nucleic acid extraction device, and further facilitating the miniaturized design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located.

[0110] The sample analyzer provided by the third purpose of the present invention is configured such that at least two workstations in the nucleic acid extraction device are distributed vertically up and down, and each workstation is configured to carry the nucleic acid extraction container to perform at least one processing action among incubation, mixing, magnetic adsorption, liquid aspiration, and liquid injection. That is, at least two workstations for carrying the nucleic acid extraction container to perform processing actions are configured to be arranged vertically up and down. This configuration scheme can make full use of the vertical space to arrange multiple workstations of the nucleic acid extraction device, thereby reducing the horizontal space occupied by the nucleic acid extraction device, and further facilitating the miniaturized design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located.

[0111] The sample analyzer provided by the fourth object of the present invention is configured to have at least two of the lysis module, cleaning module, and elution module in the nucleic acid extraction device arranged vertically up and down, that is, the functional modules used to carry the nucleic acid extraction container to perform different functional actions in sequence are arranged vertically up and down. This arrangement scheme can make full use of the vertical space to arrange the multiple functional modules of the nucleic acid extraction device, thereby reducing the horizontal space occupied by the nucleic acid extraction device, and further facilitating the miniaturized design of the sample analyzer and improving the space utilization of the laboratory where the sample analyzer is located. BRIEF DESCRIPTION OF THE DRAWINGS

[0112] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying any creative work.

[0113] FIG1 is a schematic diagram of the composition of a sample analyzer provided in Example 1 of the present invention;

[0114] FIG2 is a schematic diagram of the three-dimensional structure of the nucleic acid extraction device provided in Example 1 of the present invention from one perspective;

[0115] FIG3 is a schematic diagram of the three-dimensional structure of the nucleic acid extraction device provided in Example 1 of the present invention from another perspective;

[0116] FIG4 is a schematic diagram of a process of a nucleic acid extraction container moving to different workstations in a nucleic acid extraction apparatus according to a first embodiment of the present invention, wherein dotted arrows in the figure indicate the movement direction of the nucleic acid extraction container between the multiple workstations;

[0117] FIG5 is a schematic diagram of the distribution of the first transfer station provided in Example 1 of the present invention

[0118] FIG6 is a schematic top view of a second functional component provided in Example 1 of the present invention;

[0119] FIG7 is a schematic diagram of a three-dimensional structure of a first functional component provided in Example 1 of the present invention from one viewing angle;

[0120] FIG8 is a schematic diagram of the three-dimensional structure of the first functional component provided in Embodiment 1 of the present invention from another perspective;

[0121] FIG9 is a schematic structural diagram of a first module provided in Example 1 of the present invention;

[0122] FIG10 is a schematic structural diagram of a third module provided in Example 1 of the present invention;

[0123] FIG11 is a partial enlarged schematic diagram of FIG10;

[0124] FIG12 is a schematic structural diagram of a fourth module provided in Example 1 of the present invention;

[0125] 13 is a schematic structural diagram of a transfer assembly and a nucleic acid extraction container provided in Example 1 of the present invention;

[0126] FIG14 is a schematic structural diagram of a first pipette tip and a second pipette tip provided in Example 1 of the present invention;

[0127] FIG15 is a schematic diagram of the three-dimensional structure of the mixing component provided in Example 1 of the present invention;

[0128] FIG16 is a top view schematic diagram of the distribution of the first magnet array and the second magnet array provided in Example 1 of the present invention;

[0129] FIG17 is a schematic front view of the first magnet array and the second magnet array provided in Example 1 of the present invention;

[0130] FIG18 is a schematic top view of the first magnet array and the second magnet array provided in Example 1 of the present invention;

[0131] FIG19 is a distribution diagram of the first transfer station provided in the second embodiment of the present invention. DETAILED DESCRIPTION

[0132] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0133] In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the fact that ordinary technicians in this field can implement them. When the combination of technical solutions is mutually contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0134] This embodiment mainly optimizes the layout of the nucleic acid extraction device in the sample analyzer, fully utilizing the vertical space for the upper and lower layout, solving the problem that the nucleic acid extraction device occupies a large plane area in the sample analyzer, and further solving the problem that the sample analyzer occupies a large overall area.

[0135] Example 1:

[0136] As shown in Figures 1 to 18, a sample analyzer 1 provided in the first embodiment of the present invention includes a sample distribution device 200, a nucleic acid extraction device 100, an amplification device 300 and a detection device 400. The sample distribution device 200 is used to distribute samples; the nucleic acid extraction device 100 is used to extract nucleic acids from a liquid containing at least a sample to obtain a nucleic acid extract; the amplification device 300 is used to amplify the liquid containing at least a nucleic acid extract to obtain a test liquid; and the detection device 400 is used to detect the test liquid. The nucleic acid extraction device 100 is mainly used to extract nucleic acids from the sample to obtain purified nucleic acids. The amplification device 300 is configured to amplify the nucleic acids extracted by the nucleic acid extraction device to significantly increase the amount of nucleic acids in a relatively short period of time. The sample analyzer 1 provided in this embodiment is mainly used to obtain a test liquid by extracting and amplifying nucleic acids from the sample, and then detecting the test liquid to obtain a test result of the sample.

[0137] In one embodiment, the nucleic acid extraction device 100 extracts nucleic acid from a biological sample stored in a nucleic acid extraction container 10 using a magnetic bead method. The amplification device 300 is used to amplify the nucleic acid extracted by the nucleic acid extraction device 100, thereby significantly increasing the amount of nucleic acid in a relatively short period of time. The detection device 400 is used to detect the amplified nucleic acid.

[0138] As one embodiment, the nucleic acid extraction device 100 includes a first functional component 110, a second functional component 120, and a transfer component 130. The first functional component 110 is disposed above or below the second functional component 120. The transfer component 130 is used to transfer the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120 to extract nucleic acid and obtain a nucleic acid extract. The first functional component 110 is used to at least magnetically adsorb and aspirate the liquid in the nucleic acid extraction container 10, and the second functional component 120 is used to at least incubate the liquid in the nucleic acid extraction container 10. The first functional component 110 and the second functional component 120 are two different functional components of the nucleic acid extraction device 100, wherein the first functional component 110 is primarily used to magnetically adsorb and aspirate the liquid in the nucleic acid extraction container 10, and the second functional component 120 is primarily used to incubate the liquid in the nucleic acid extraction container 10. In this embodiment, the first functional component 110 and the second functional component 120 for performing different functional actions on the liquid in the nucleic acid extraction container 10 are arranged in an upper and lower position in the vertical direction, and the nucleic acid extraction container 10 is transferred up and down between the first functional component 110 and the second functional component 120 by the transfer component 130. When the liquid in the nucleic acid extraction container 10 needs to be incubated, the transfer component 130 transfers the nucleic acid extraction container 10 to the second functional component 120; when the liquid in the nucleic acid extraction container 10 needs to be magnetically adsorbed and aspirated, the transfer component 130 transfers the nucleic acid extraction container 10 to the first functional component 110. This arrangement scheme can make full use of the vertical space to arrange the different functional components of the nucleic acid extraction device 100, thereby reducing the horizontal occupied space of the nucleic acid extraction device 100, and further facilitating the miniaturization design of the sample analyzer 1, and improving the space utilization of the laboratory where the sample analyzer 1 is located.

[0139] As an embodiment, the sample analyzer 1 also includes a pipetting device 700. The sample dispensing device 200 is used to draw at least a portion of the sample from the sample container and dispense it into the nucleic acid extraction container 10 provided by the second consumables supply device; the nucleic acid extraction device 100 is used to extract nucleic acid from the liquid containing at least the sample and reagent in the nucleic acid extraction container 10 to obtain a nucleic acid extract solution, wherein the reagent contains magnetic beads; the pipetting device 700 is used to transfer the nucleic acid extract solution in the nucleic acid extraction container 10 to an amplification reaction container; and the amplification device 300 is used to amplify the liquid containing at least the nucleic acid extract solution in the amplification reaction container to obtain a test solution. The sample container, the nucleic acid extraction container 10, and the amplification reaction container are three different containers, and the pipetting device 700 is primarily used to transfer the nucleic acid extract solution between the nucleic acid extraction container 10 and the amplification reaction container. The sample container is primarily used to hold samples collected from patients, i.e., the sample container is primarily used to provide a storage space for the sample. The nucleic acid extraction container 10 is primarily used to carry samples for nucleic acid extraction, i.e., the nucleic acid extraction container 10 is primarily used to provide a storage space for nucleic acid extraction from the sample. The amplification reaction vessel is primarily used to carry nucleic acid extracts for amplification reactions. This means the amplification reaction vessel primarily serves as a site for the amplification reaction. The nucleic acid extraction vessel 10 and the amplification reaction vessel are two different consumables of the sample analyzer 1. This allows the nucleic acid extraction vessel 10 to be designed into a shape that facilitates nucleic acid extraction, and the amplification reaction vessel to be designed into a shape that facilitates amplification reactions.

[0140] As an embodiment, the first functional component 110 includes a first carrying member 111, a first pipetting member 118 and a first magnetic attraction member 119. The first carrying member 111 is used to carry the nucleic acid extraction container 10. The first magnetic attraction member 119 is used to perform a first magnetic adsorption on the liquid containing at least a sample and a reagent in the nucleic acid extraction container 10 located on the first carrying member 111 to adsorb the magnetic beads in the nucleic acid extraction container 10. The first pipetting member 118 is used to perform a first liquid aspiration action on the liquid in the nucleic acid extraction container 10 located on the first carrying member 111 and subjected to the first magnetic adsorption by the first magnetic attraction member 119. The transfer component 130 is used to transfer the nucleic acid extraction container 10 between the first carrying member 111 and the second functional component 120. The magnetic adsorption includes the first magnetic adsorption, and the liquid aspiration action includes the first liquid aspiration action. The first magnetic attraction member 119 includes at least one magnet. During the incubation process, the nucleic acid in the biological sample will be combined with the nucleic acid. The first magnetic attraction member 119 is primarily used to perform a first magnetic attraction on the liquid in the nucleic acid extraction container 10 placed on the first supporting member 111. This first magnetic attraction attracts the combination of magnetic beads and nucleic acids. The first liquid transfer member 118 is primarily used to remove waste liquid from the nucleic acid extraction container 10 and discharge it. That is, the first liquid transfer member 118 is primarily used to remove the liquid in the nucleic acid extraction container 10 except for the magnetic beads and the combination of magnetic beads and nucleic acids and discharge it as waste liquid, thereby facilitating the purification of nucleic acids.

[0141] As an embodiment, the first functional component 110 further includes a liquid injection component, which is used to perform a first liquid injection operation of injecting a cleaning liquid into the nucleic acid extraction container 10 located on the first supporting member 111. The liquid injection component is mainly used to add the cleaning liquid into the nucleic acid extraction container 10 to facilitate the subsequent cleaning function.

[0142] Specifically, the liquid injection component is mainly used to perform the first liquid injection action of adding a cleaning liquid to the nucleic acid extraction container 10 after completing the first liquid aspiration action, and the added cleaning liquid is used to clean the liquid in the nucleic acid extraction container 10. The second functional component 120 is used to carry the nucleic acid extraction container 10 to perform an incubation action, including: the second functional component 120 is used to carry the nucleic acid extraction container 10 to perform a lysis incubation action and a washing incubation action. The transfer component 130 is used to transfer the nucleic acid extraction container 10 after completing the lysis incubation action from the second functional component 120 to the first supporting component 111, and is used to transfer the nucleic acid extraction container 10 after completing the first liquid aspiration action and the first liquid injection action in sequence from the first supporting component 111 to the second functional component 120 to perform a washing incubation action.

[0143] As an embodiment, the number of first supporting members 111 is at least two, and each first supporting member 111 is correspondingly provided with at least one liquid injection member and at least one first liquid transfer member 118. At least two first supporting members 111 form at least two workstations arranged in sequence. After a first supporting member 111 completes a first magnetic adsorption, a first liquid aspiration action, and a first liquid injection action, the nucleic acid extraction container 10 will first be dispatched to the second functional component 120 for cleaning and incubation, and then dispatched to the next first supporting member 111 to perform the next first magnetic adsorption, a first liquid aspiration action, and a first liquid injection action. In this embodiment, the nucleic acid extraction device 100 performs at least two magnetic adsorption, waste liquid aspiration, and cleaning liquid injection operations on the liquid in the nucleic acid extraction container 10. Then, the liquid in the nucleic acid extraction container 10 is correspondingly cleaned and magnetically separated at least twice. This is conducive to cleaning and discharging non-nucleic acid components in the sample, thereby ensuring the degree of purification of the nucleic acid. Of course, in specific applications, as an alternative embodiment, the number of first supporting members 111 can also be only one.

[0144] As an embodiment, the number of injection components and the number of first pipetting components 118 are the same as the number of first supporting components 111, that is, each first supporting component 111 is provided with one injection component and one first pipetting component 118. This facilitates the parallel operation of multiple nucleic acid extraction containers 10 on the first functional component 110 and reduces the movement span of the injection components and the first pipetting components 118. Of course, in specific applications, as an alternative embodiment, two first supporting components 111 may also share the same injection component and / or the same first pipetting component 118.

[0145] As an embodiment, the number of first carrying members 111, the number of injection members and the number of first pipetting members 118 are all two. Of course, in specific applications, the number of first carrying members 111, the number of injection members and the number of first pipetting members 118 can also be three or even more.

[0146] As an embodiment, the first functional component 110 further includes a second supporting member 112, a second liquid transfer member 114, and a second magnetic member 115. The second supporting member 112 is used to support the nucleic acid extraction container 10. The second magnetic member 115 is used to perform a second magnetic adsorption on the liquid containing at least the cleaning liquid in the nucleic acid extraction container 10 located on the second supporting member 112. The second liquid transfer member 114 is used to perform a second liquid aspiration action on the liquid in the nucleic acid extraction container 10 located on the second supporting member 112 and subjected to the second magnetic adsorption by the second magnetic member 115. The transfer component 130 is also used to transfer the nucleic acid extraction container 10 between the second supporting member 112 and the second functional component 120. The magnetic adsorption further includes a second magnetic adsorption, and the liquid aspiration action further includes a second liquid aspiration action. The first supporting member 111 and the second supporting member 112 form two workstations arranged in sequence. The nucleic acid extraction container 10 will be dispatched to the second supporting member 112 to perform the second magnetic adsorption and second liquid aspiration actions only after all the first supporting members 111 have completed the first magnetic adsorption, the first liquid aspiration action, and the first liquid injection action. The second pipetting member 114 is used to perform a second pipetting operation on the nucleic acid extraction container 10 that is located on the second carrying member 112 and has completed all the first pipetting operations and the first injection operations in sequence.

[0147] The difference between the workstation formed by the first supporting member 111 and the workstation formed by the second supporting member 112 is that the workstation is only used to perform magnetic adsorption and suction and discharge of waste liquid, and does not need to perform liquid injection; while the workstation formed by the first supporting member 111 needs to inject cleaning liquid after completing magnetic adsorption and suction and discharge of waste liquid.

[0148] As an embodiment, the sample analyzer 1 further includes a first lifting member 116; the first lifting member 116 is connected to the second magnetic member 115, and the first lifting member 116 is used to drive the second magnetic member 115 to move up and down relative to the nucleic acid extraction container 10. In this embodiment, the second magnetic member 115 performs a downward movement, which can drag the magnetic binder (a combination of magnetic beads and nucleic acid) in the nucleic acid extraction container 10 downward, so that the magnetic binder is gathered at the bottom of the nucleic acid extraction container 10. This helps to avoid the undesirable phenomenon that the magnetic binder is too dispersed, resulting in the elution reagent being unable to completely submerge the magnetic binder, helps to ensure the reliability of the subsequent elution operation, and helps to prevent the second pipetting member 114 from contacting or carrying away the magnetic binder during aspiration.

[0149] Specifically, the first lifting component 116 is used to: drive the second magnetic component 115 to move downward after the transfer component 130 places the nucleic acid extraction container 10 on the second carrying component 112 and before the second pipetting component 114 performs the second liquid aspiration action; the second functional component 120 is also used to carry the nucleic acid extraction container 10 to perform a drying and incubation action; the transfer component 130 is also used to transfer the nucleic acid extraction container 10 after completing the washing and incubation action from the second functional component 120 to the second carrying component 112, and to transfer the nucleic acid extraction container 10 after completing the second liquid aspiration action from the second carrying component 112 to the second functional component 120 to perform a drying and incubation action.

[0150] In one embodiment, the first functional assembly 110 further includes a third supporting member 113 and a third pipetting member 117. The third supporting member 113 is used to support the nucleic acid extraction container 10, and the third pipetting member 117 is used to perform the second liquid injection operation of injecting elution reagent into the nucleic acid extraction container 10 located on the third supporting member 113. The transfer assembly 130 is also used to transfer the nucleic acid extraction container 10 between the third supporting member 113 and the second functional assembly 120. The first supporting member 111, the second supporting member 112, and the third supporting member 113 form three sequentially arranged workstations. The nucleic acid extraction container 10 is dispatched to the third supporting member 113 for the second liquid injection operation only after all first supporting members 111 have completed the first magnetic attraction, first liquid aspiration, and first liquid injection operation, and after the second supporting member 112 has completed the second magnetic attraction and second liquid aspiration operations. The workstation formed by the third supporting member 113 is used only for the liquid injection operation of injecting elution reagent, and does not require magnetic attraction or waste liquid aspiration and discharge operations.

[0151] Specifically, the third pipetting component 117 is used to perform the second liquid injection action of injecting elution reagent into the nucleic acid extraction container 10 located on the third carrying component 113 and after completing the second liquid aspiration action; the second functional component 120 is also used to carry the nucleic acid extraction container 10 to perform the elution incubation action; the transfer component 130 is also used to transfer the nucleic acid extraction container 10 after completing the drying and incubation action from the second functional component 120 to the third carrying component 113, and to transfer the nucleic acid extraction container 10 after completing the second liquid injection action from the third carrying component 113 to the second functional component 120 to perform the elution incubation action.

[0152] As an embodiment, the first bearing member 111, the second bearing member 112 and the third bearing member 113 are arranged side by side in the horizontal direction, that is, the first bearing member 111, the second bearing member 112 and the third bearing member 113 are arranged in a row in the horizontal direction, which is conducive to simplifying the movement trajectory of the transfer component 130.

[0153] As an embodiment, the second bearing member 112 is disposed between the first bearing member 111 and the third bearing member 113 along the horizontal direction.

[0154] As an embodiment, the two first bearing members 111 , the second bearing member 112 and the third bearing member 113 are sequentially arranged side by side in the horizontal direction.

[0155] In one embodiment, the nucleic acid extraction container 10 is formed with reaction wells for holding samples and reagents for nucleic acid extraction. The sample dispensing device 200 is used to aspirate the sample from the sample container and dispense at least a portion of the aspirated sample into the reaction wells. The nucleic acid extraction device 100 is used to extract nucleic acids from the liquid in the reaction wells containing at least the sample and reagents. The pipetting device 700 is used to transfer the nucleic acid extraction liquid in the reaction wells to the amplification reaction container.

[0156] As an embodiment, the nucleic acid extraction container 10 is also formed with at least one pipette tip hole, and each pipette tip hole is used to carry a pipette tip. The nucleic acid extraction device 100 is also used to perform pipetting through at least one pipette tip on the nucleic acid extraction container 10. After a pipette tip completes the corresponding pipetting action in a detection item, it is released to the recovery position together with the nucleic acid extraction container 10 for recovery, so as to save the cleaning process and help improve the detection efficiency. In addition, the pipette tip is directly placed in the nucleic acid extraction container 10. On the one hand, there is no need to set up a separate storage bin and scheduling mechanism for the pipette tip in the sample analyzer 1; on the other hand, it is beneficial to shorten the scheduling stroke of the pipette tip when using the pipette tip for pipetting, thereby helping to improve the pipetting efficiency.

[0157] In one embodiment, the nucleic acid extraction container 10 further includes a first pipette tip hole for receiving a first pipette tip 20. The first pipette member 118 is configured to perform a first aspiration operation on the liquid in the reaction well via the first pipette tip 20. In this embodiment, the first pipette member 118 aspirates waste liquid during the nucleic acid extraction process via the pipette tip, eliminating the need for washing the first pipette member 118. This improves the efficiency of nucleic acid extraction and helps prevent cross-contamination caused by inadequate cleaning.

[0158] In one embodiment, the first pipetting assembly 118 includes a first tip connection component and a first suction and discharge power component. The first tip connection component is configured to be detachably connected to the first pipetting tip 20, enabling liquid to be aspirated through the first pipetting tip 20 under the power provided by the first suction and discharge power component. The first tip connection component is primarily configured to plug and mate with the first pipetting tip 20 to achieve clamping of the first pipetting tip 20. The first suction and discharge power component is primarily configured to provide driving force for the first pipetting tip 20 to aspirate and discharge liquid.

[0159] As an embodiment, the first pipetting component 118 further includes a first moving power component, which is used to drive the first tip connecting component to move so that the first tip connecting component moves to different working positions, such as a standby position, a tip clamping position, a liquid aspiration position, a liquid discharge position, a tip release position, etc.

[0160] As an embodiment, the first moving power component is used to drive the first tip connecting component to move, so that the first tip connecting component moves to the top of the first tip hole, the first tip connecting component is connected to the first pipette tip 20, and the first tip connecting component drives the first pipette tip 20 to move and insert into the reaction hole; the first suction and discharge power component is used to provide driving force for the first pipette tip 20 to perform the suction action after the first pipette tip 20 is inserted into the reaction hole.

[0161] In one embodiment, the nucleic acid extraction container 10 further includes a second pipette tip hole for receiving a second pipette tip 30. The third pipette member 117 is used to perform a second liquid injection operation into the reaction well via the second pipette tip 30. In this embodiment, the third pipette member 117 aspirates waste liquid during the nucleic acid extraction process via the pipette tip, eliminating the need for washing the third pipette member 117 and improving nucleic acid extraction efficiency.

[0162] In one embodiment, the volume of the first pipette tip 20 is greater than that of the second pipette tip 30. The first pipette tip 20 is used to aspirate and discharge waste liquid, aspirating relatively large amounts; the second pipette tip 30 is used to aspirate and discharge elution reagent, aspirating relatively small amounts. Here, setting the volume of the first pipette tip larger than that of the second pipette tip 30 facilitates, on the one hand, allowing the first pipette tip to aspirate waste liquid from the reaction well in one go, reducing the number of aspiration and discharge actions of the first pipette tip, thereby ensuring efficient waste liquid aspiration and discharge; and, on the other hand, ensuring the accuracy of aspiration and injection of the elution reagent.

[0163] As an embodiment, the third pipetting component 117 includes a second tip connecting component and a second suction and discharge power component. The second tip connecting component is used to be detachably connected to the second pipetting tip 30 to aspirate liquid through the second pipetting tip 30 under the action of the power provided by the second suction and discharge power component.

[0164] As an embodiment, the third pipetting component 117 further includes a second movable power component, which is used to drive the second tip connecting component to move so that the second tip connecting component moves to different working positions, such as a standby position, a tip clamping position, a liquid aspiration position, a liquid discharge position, a tip release position, etc.

[0165] Specifically, the second moving power component is used to drive the second tip connecting component to move, so that the second tip connecting component moves to the top of the second tip hole, the second tip connecting component is connected to the second pipette tip 30, the second tip connecting component drives the second pipette tip 30 to move to the elution reagent aspiration position, and the second tip connecting component drives the second pipette tip 30 to move and insert into the reaction well; the second suction and discharge power component is used to provide driving force for the second pipette tip 30 to perform the aspiration action after the second pipette tip 30 moves to the elution reagent aspiration position, and to provide driving force for the second pipette tip 30 to perform the second liquid injection action after the second pipette tip 30 is inserted into the reaction well.

[0166] In one embodiment, the nucleic acid extraction container 10 further includes a waste liquid well. The first and second pipetting members 118, 114 are further configured to discharge liquid drawn from the reaction wells into the waste liquid well. In this embodiment, the waste liquid well is formed directly on the nucleic acid extraction container 10, thereby shortening the path for waste liquid discharge during the nucleic acid extraction process.

[0167] As an embodiment, the second pipetting component 114 includes a third tip connecting component, a third suction and discharge power component and a third moving power component. The third tip connecting component is used to connect with the first pipetting tip 20. The third moving power component is used to drive the third tip connecting component to move, so that the third tip connecting component moves to the top of the first tip hole, the third tip connecting component is connected to the first pipetting tip 20, and the third tip connecting component drives the first pipetting tip 20 to move and insert into the reaction hole; the third suction and discharge power component is used to provide driving force for the first pipetting tip 20 to perform the suction action after the first pipetting tip 20 is inserted into the reaction hole.

[0168] As an embodiment, the injection component includes an injection needle, an injection pipeline and a fourth suction and discharge power component. The injection pipeline is used to connect the injection needle and the cleaning liquid container. The fourth suction and discharge power component is used to drive the injection pipeline to draw the cleaning liquid from the cleaning liquid container and transport it to the injection needle and inject it into the reaction hole of the nucleic acid extraction container 10 located on the first supporting component 111 through the injection needle.

[0169] As an embodiment, the sample analyzer 1 further includes a frame assembly; the first functional assembly 110 includes a first connecting plate 1001, a second connecting plate 1002, a third connecting plate 1003, a fourth connecting plate 1004, two first bearing members 111, two first pipetting members 118, two injection members, two first magnetic members 119, a second bearing member 112, a second pipetting member 114, a second magnetic member 115, a third bearing member 113 and a third pipetting member 117; the first connecting plate 1001 is connected to the first bearing member 111, the first pipetting member 118, the injection member and the first magnetic member 119 to form a first module 101, and the first module 101 is connected to the first The connecting plate 1001 is connected to the frame assembly; the second connecting plate 1002 is connected to another first bearing member 111, another first pipetting member 118, another injection member, and another first magnetic member 119 to form a second module 102, and the second module 102 is connected to the frame assembly through the second connecting plate 1002; the third connecting plate 1003 is connected to the second bearing member 112, the second pipetting member 114, and the second magnetic member 115 to form a third module 103, and the third module 103 is connected to the frame assembly through the third connecting plate 1003; the fourth connecting plate 1004 is connected to the third bearing member 113 and the third pipetting member 117 to form a fourth module 104, and the fourth module 104 is connected to the frame assembly through the fourth connecting plate 1004.

[0170] As an implementation manner, the first module 101 , the second module 102 , the third module 103 , and the fourth module 104 are arranged side by side in sequence along the horizontal direction.

[0171] As an implementation manner, the first module 101 , the second module 102 , the third module 103 , and the fourth module 104 are sequentially arranged side by side along a straight line.

[0172] In one embodiment, the second functional component 120 includes a fourth supporting member 121 and a heating member. The fourth supporting member 121 is used to support the nucleic acid extraction container 10 for incubation, and the heating member is used to heat the liquid containing at least the sample and reagent in the nucleic acid extraction container 10 located on the fourth supporting member 121. The heating member is primarily used to provide a suitable incubation environment temperature for the liquid in the nucleic acid extraction container 10, thereby ensuring incubation efficiency.

[0173] As an embodiment, the fourth supporting member 121 includes a lysis zone 1211, a washing zone 1212, a drying zone 1213 and an elution zone 1214. The lysis zone 1211, the washing zone 1212, the drying zone 1213 and the elution zone 1214 are respectively formed with a accommodating cavity for placing the nucleic acid extraction container 10. The transfer assembly 130 is used to transfer the nucleic acid extraction container 10 between the lysis zone 1211, the washing zone 1212 and the first supporting member 111, between the washing zone 1212, the drying zone 1213 and the second supporting member 112, and between the drying zone 1213, the elution zone 1214 and the third supporting member 113. Specifically, the transfer assembly 130 is used to: transfer the nucleic acid extraction container 10 located in the lysis zone 1211 to the first supporting member 111, transfer the nucleic acid extraction container 10 located in the first supporting member 111 to the washing zone 1212, transfer the nucleic acid extraction container 10 located in the washing zone 1212 to the second supporting member 112, transfer the nucleic acid extraction container 10 located in the second supporting member 112 to the drying zone 1213, transfer the nucleic acid extraction container 10 located in the drying zone 1213 to the third supporting member 113, and transfer the nucleic acid extraction container 10 located in the third supporting member 113 to the elution zone 1214. In this embodiment, the nucleic acid extraction performed by the nucleic acid extraction device 100 mainly includes lysis, washing, drying and elution. The lysis step is performed in the lysis zone 1211. The lysis step mainly uses a lysis reagent to rupture the cells in the sample to release nucleic acids, and the released nucleic acids are adsorbed on the magnetic beads in the nucleic acid extraction container 10. The washing step is performed in the washing area 1212. The washing step mainly uses a cleaning solution (or washing solution) to remove unnecessary components in the sample, such as washing away various impurities such as proteins, lipids and other substances remaining on and between the magnetic beads. The drying step is performed in the drying area 1213. The drying step mainly dries the washed liquid by heating. The elution step is performed in the elution area 1214. The elution step mainly uses an elution reagent to separate the nucleic acid from the magnetic beads, so that purified nucleic acid can be obtained. Of course, in specific applications, the steps of nucleic acid extraction are not limited to this. For example, as an alternative embodiment, it is also possible not to set up the drying area 1213 and the drying step.

[0174] As an embodiment, the fourth supporting member 121 is formed with two washing zones 1212 , which are respectively a first washing zone 1212 and a second washing zone 1212 . The first functional component 110 includes two first supporting members 111 . The transfer component 130 transfers the nucleic acid extraction container 10 located in the lysis zone 1211 to the first supporting member 111, transfers the nucleic acid extraction container 10 located in the first supporting member 111 to the washing zone 1212, and transfers the nucleic acid extraction container 10 located in the washing zone 1212 to the second supporting member 112, including: transferring the nucleic acid extraction container 10 that has completed lysis and incubation in the lysis zone 1211 to a first supporting member 111, transferring the nucleic acid extraction container 10 that has completed the first liquid aspiration action and the first liquid injection action on the first supporting member 111 to the first washing zone 1212, transferring the nucleic acid extraction container 10 that has completed cleaning and incubation in the first washing zone 1212 to another first supporting member 111, transferring the nucleic acid extraction container 10 that has completed the first liquid aspiration action and the first liquid injection action on the other first supporting member 111 to the second washing zone 1212, and transferring the nucleic acid extraction container 10 that has completed cleaning and incubation in the second washing zone 1212 to the second supporting member 112. In this embodiment, two washing zones 1212 are provided to ensure the cleanliness of the washing, thereby ensuring the degree of purification of the nucleic acid. Of course, in specific applications, the number of washing zones 1212 is not limited thereto, for example, it may be one, three, or even more.

[0175] As an embodiment, the fourth supporting member 121 includes an incubation tray, and the heating member is used to heat the nucleic acid extraction container 10 on the incubation tray; the incubation tray is formed with a lysis area 1211, a washing area 1212, a drying area 1213 and an elution area 1214, and a first transfer station 1215 is formed on the incubation tray. The incubation tray is used to dispatch the nucleic acid extraction containers 10 located in the lysis area 1211, the washing area 1212, the drying area 1213 and the elution area 1214 to the first transfer station 1215 respectively, and the transfer assembly 130 is used to dispatch the nucleic acid extraction container 10 located in the first transfer station 1215 to the first functional component 110. In this embodiment, the transfer assembly 130 only takes and places the nucleic acid extraction container 10 on the fourth supporting member 121 at one transfer station (i.e., the first transfer station 1215), which helps to simplify the motion trajectory and structural complexity of the transfer assembly 130.

[0176] As an embodiment, the fourth bearing member 121 is disc-shaped, and the fourth bearing member 121 is formed with a plurality of accommodating cavities arranged along the horizontal circumferential direction, each accommodating cavity being used to accommodate a nucleic acid extraction container 10. The first functional component 110 includes a first bearing member 111, a second bearing member 112, a third bearing member 113 and at least three connecting plates, the at least three connecting plates being arranged in sequence and spaced apart, the first bearing member 111, the second bearing member 112, and the third bearing member 113 being respectively mounted on a corresponding connecting plate, the first bearing member 111 being used to carry the nucleic acid extraction container 10 to perform a first liquid aspiration action and a first liquid injection action of injecting a cleaning solution, the second bearing member 112 being used to carry the nucleic acid extraction container 10 to perform a second liquid aspiration action, and the third bearing member 113 being used to carry the nucleic acid extraction container 10 to perform a second liquid injection action of injecting an elution reagent.

[0177] In one embodiment, the sample analyzer 1 further includes a frame assembly, on which the nucleic acid extraction device 100 is mounted. A fourth supporting member 121 is movably mounted on the frame assembly to support the nucleic acid extraction container 10 for incubation. The fourth supporting member 121 is formed with at least one first transfer station 1215 and multiple accommodating cavities, each for accommodating a nucleic acid extraction container 10. A first driving member 122 is configured to drive the fourth supporting member 121 relative to the frame assembly so that the fourth supporting member 121 moves the multiple nucleic acid extraction containers 10 to the first transfer station 1215. A first supporting member 111 is fixed to the frame assembly. The transfer assembly 130 is configured to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the first supporting member 111. A first pipetting member 118 is configured to perform a first liquid aspiration operation on the nucleic acid extraction container 10 located on the first supporting member 111. In this embodiment, the supporting member of the first functional component 110 is stationary relative to the frame assembly, while the supporting member of the second functional component 120 is movable relative to the frame assembly.

[0178] In one embodiment, the first supporting member 111, the second supporting member 112, and the third supporting member 113 are mounted on the frame assembly in a stationary manner relative to the frame assembly to support the nucleic acid extraction container 10 and perform the first, second, and second liquid injection operations, respectively. That is, the second supporting member 112 and the third supporting member 113 are also mounted on the frame assembly in a stationary manner relative to the frame assembly to support the nucleic acid extraction container 10. The second pipetting member 114 is used to perform the second pipetting operation on the nucleic acid extraction container 10 located on the second supporting member 112 after the first liquid injection operation has been completed, and the third pipetting member 117 is used to perform the second liquid injection operation of injecting elution reagent into the nucleic acid extraction container 10 located on the third supporting member 113 after the second liquid injection operation has been completed. The transfer assembly 130 is further used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the second supporting member 112, and between the first transfer station 1215 and the third supporting member 113.

[0179] As an embodiment, the first bearing member 111, the second bearing member 112 and the third bearing member 113 are arranged side by side in sequence along a horizontal straight line direction; the transfer assembly 130 includes a single first clamping member and a single second driving member, the first clamping member is used to clamp and release the nucleic acid extraction container 10, and the second driving member is used to drive the first clamping member to perform three-dimensional linear motion so that the first clamping member moves to the first transfer station 1215, the first bearing member 111, the second bearing member 112 and the third bearing member 113 respectively to clamp and release the nucleic acid extraction container 10.

[0180] As an embodiment, the sample analyzer 1 further includes a controller 600, which is configured to: control the transfer component 130 to dispatch the nucleic acid extraction container 10 after the incubation action is performed in the lysis area 1211 to the first carrying member 111; control the first pipetting component 118 to perform a first liquid aspiration action on the liquid in the nucleic acid extraction container 10 in the first carrying member 111 and control the liquid injection component to perform a first liquid injection action on the nucleic acid extraction container 10 in the first carrying member 111; control the transfer component 130 to transport the nucleic acid extraction container 10 after the first liquid injection action to the washing area 1212 to perform the incubation action; control the transfer component 130 to transport the nucleic acid extraction container 10 after the incubation action is performed in the washing area 1212 to perform the incubation action. To the second carrying member 112; control the second pipetting member 114 to perform a second liquid aspiration action on the liquid in the nucleic acid extraction container 10 in the second carrying member 112; control the transfer component 130 to transport the nucleic acid extraction container 10 after the second liquid aspiration action to the drying area 1213 to perform a drying action on the nucleic acid extraction container 10; control the transfer component 130 to transport the nucleic acid extraction container 10 after the drying action in the drying area 1213 to the third carrying member 113; control the third pipetting member 117 to perform a second liquid injection action on the nucleic acid extraction container 10 in the third carrying member 113; control the transfer component 130 to transport the nucleic acid extraction container 10 after the second liquid injection action to the elution area 1214 to perform an elution action.

[0181] As an embodiment, the nucleic acid extraction method includes the following steps: a lysis and incubation step, a first aspiration and injection step, a first washing and incubation step, a second aspiration and injection step, a second washing and incubation step, a single aspiration step, a drying step, an elution reagent injection step, and an elution and incubation step. The lysis and incubation step mainly includes a lysis and incubation step of the liquid in the nucleic acid extraction container 10 in the lysis zone 1211. Specifically, the lysis system can be constructed in other devices of the previous step, for example, by adding a lysis reagent and a sample to the nucleic acid extraction container 10, and transferring the nucleic acid extraction container 10 with the lysis reagent and sample to the accommodating chamber of the lysis zone 1211, and heating the sample to be lysed in the lysis zone 1211 by a heating component. After the lysis and incubation steps are completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform a first aspiration and injection step to assist in the subsequent washing process. The first aspiration and injection step includes: magnetically adsorbing the magnetic binding body in the nucleic acid extraction container 10. After the magnetic binding body is adsorbed, the waste liquid in the nucleic acid extraction container 10 is aspirated and discharged, and a cleaning liquid is injected after the waste liquid is aspirated. After the first aspiration and injection step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120, and the following first washing and incubation step is performed: the liquid in the nucleic acid extraction container 10 is washed and incubated in the first washing area 1212. After the first washing and incubation step in the first washing area 1212 is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 to perform a second aspiration and injection step. The second aspiration and injection step is similar to the first aspiration and injection step and will not be described in detail here. After the second aspiration and injection step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 for the second washing and incubation step. The second washing and incubation step is similar to the first washing and incubation step and will not be described in detail here. After the second washing and incubation step is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 for the single aspiration step. The single aspiration step includes magnetically adsorbing the magnetic binder within the nucleic acid extraction container 10, and then aspirating and discharging the waste liquid within the nucleic acid extraction container 10 after magnetic bead adsorption. After the single aspiration step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 for the drying step. The drying step includes drying the liquid within the nucleic acid extraction container 10 in the drying area 1213. After the drying step is completed, the nucleic acid extraction container 10 is transferred from the second functional component 120 to the first functional component 110 for the elution reagent dispensing step. The elution reagent dispensing step includes injecting the elution reagent into the reaction wells of the nucleic acid extraction container 10. After the elution reagent dispensing step is completed, the nucleic acid extraction container 10 is transferred from the first functional component 110 to the second functional component 120 to perform the elution incubation step, which includes: performing an elution incubation step on the liquid in the nucleic acid extraction container 10 in the elution area 1214.

[0182] In one embodiment, the bottom of the accommodating chamber in the elution zone 1214 is located at a higher position than the bottom of the accommodating chamber in the lysis zone 1211, higher than the bottom of the accommodating chamber in the washing zone 1212, and higher than the bottom of the accommodating chamber in the drying zone 1213. Because the amount of liquid in the nucleic acid extraction container 10 during the lysis and washing steps is greater than the amount of liquid in the nucleic acid extraction container 10 during the elution step, in this embodiment, the height of the nucleic acid extraction container 10 in the elution zone 1214 is set higher than the heights of the nucleic acid extraction container 10 in the lysis zone 1211, the washing zone 1212, and the drying zone 1213. This allows the magnetic binders in the nucleic acid extraction container 10 in the elution zone 1214 to be concentrated as much as possible at the bottom of the nucleic acid extraction container 10, thereby preventing nucleic acid loss.

[0183] In one embodiment, the bottom of the accommodating chamber in the lysis zone 1211 is located at the same height as the bottom of the accommodating chamber in the washing zone 1212 and the bottom of the accommodating chamber in the drying zone 1213. The height of the nucleic acid extraction container 10 in the lysis zone 1211 is the same as that in the washing zone 1212 and the drying zone 1213. Of course, in specific applications, the height of the nucleic acid extraction container 10 in at least two of the lysis zone 1211, the washing zone 1212, and the drying zone 1213 may also be set to be different.

[0184] As an embodiment, the second functional component 120 further includes a first driving member 122, which is configured to drive the fourth supporting member 121 to move, thereby causing the fourth supporting member 121 to move the nucleic acid extraction container 10. The first driving member 122 can drive the nucleic acid extraction containers 10 located in the lysis zone 1211, the washing zone 1212, the drying zone 1213, and the elution zone 1214 to be dispatched to the first transfer station 1215, thereby enabling the transfer assembly 130 to transfer the nucleic acid extraction containers 10 between the first transfer station 1215 and the first functional component 110.

[0185] As an embodiment, the second functional component 120 further includes a mixing member 140, which is used to mix the liquid containing at least the sample and reagent in the nucleic acid extraction container 10 located on the fourth supporting member 121. This facilitates sufficient mixing of the sample and reagent, thereby facilitating sufficient nucleic acid capture, cleaning, and elution.

[0186] In one embodiment, the mixing element 140 includes multiple magnets, which are alternately arranged on opposite sides of the horizontal trajectory of the nucleic acid extraction container 10. The magnets generate a magnetic field, which exerts a magnetic force on the magnetic beads and magnetic binders in the nucleic acid extraction container 10. In this embodiment, the use of magnets to mix the liquid in the nucleic acid extraction container 10 prevents direct contact between the mixing element 140 and the liquid in the nucleic acid extraction container 10, thereby preventing contamination of the mixing element 140.

[0187] In one embodiment, the mixing member 140 includes at least a first magnet array 141 and a second magnet array 142 extending horizontally. Each magnet array comprises a plurality of magnets spaced apart and mounted on a magnet support 143. The fourth supporting member 121 includes a container receiving portion for accommodating the nucleic acid extraction container 10. The container receiving portion is formed with a plurality of cavities for accommodating the nucleic acid extraction container 10. The first magnet array 141 includes a plurality of first magnets 1411 arranged in an array. The second magnet array 142 includes a plurality of second magnets 1421 distributed in an array.

[0188] As an embodiment, the first magnet array 141 and the second magnet array 142 are parallel to each other in the horizontal extension direction R. The first magnet array 141 and the second magnet array 142 being parallel to each other in the horizontal extension direction R means that the magnets in the first magnet array 141 and the second magnet array 142 are continuously distributed in the horizontal extension direction R. In particular, the center line of each magnet in the first magnet array 141 extends in the horizontal extension direction R, and the center line of each magnet in the second magnet array 142 extends in the horizontal extension direction R. However, the magnets themselves may be arranged tilted relative to the horizontal extension direction R.

[0189] In one embodiment, the fourth supporting member 121 and the mixing member 140 are arranged relative to each other such that, when the nucleic acid extraction container 10 is received in the container receiving portion, at least the bottom of the nucleic acid extraction container 10 is located between the first magnet array 141 and the second magnet array 142. That is, the first magnet array 141 and the second magnet array 142 are horizontally distributed on opposite sides of the motion trajectory of the nucleic acid extraction container 10. The fourth supporting member 121 and the mixing member 140 are capable of horizontal movement relative to each other, enabling the mixing member 140 to mix the liquid containing at least the sample and magnetic beads in the nucleic acid extraction container 10 received in the container receiving portion.

[0190] As an embodiment, the fourth supporting member 121 and the mixing member 140 are further arranged relative to each other: when the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0191] Because the fourth supporting member 121 and the mixing member 140 can move horizontally relative to each other, the fourth supporting member 121 can drive the nucleic acid extraction container 10 received in the container receiving portion and located between the first magnet array 141 and the second magnet array 142 to move horizontally relative to the first magnet array 141 and the second magnet array 142, thereby generating a horizontally varying magnetic field for the nucleic acid extraction container 10 received in the container receiving portion. In addition, because the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 are at different vertical heights from the bottom of the nucleic acid extraction container 10 received in the container receiving portion, thereby also generating a vertically varying magnetic field for the nucleic acid extraction container 10 received in the container receiving portion. By generating a magnetic field that changes in the horizontal and vertical directions, the magnetic beads in the nucleic acid extraction container 10 placed in the changing magnetic field are spatially distributed as much as possible in the entire liquid of the nucleic acid extraction container 10, or the temporal movement trajectory of the magnetic beads is spread throughout the entire liquid of the nucleic acid extraction container 10. In particular, the magnetic beads in the nucleic acid extraction container 10 placed in the changing magnetic field can perform three-dimensional motion (for example, circumferentially and axially), thereby achieving sufficient mixing of the magnetic beads.

[0192] When the nucleic acid extraction device 100 is in operation, the relative horizontal movement between the fourth supporting member 121 and the mixing member 140 is uninterrupted and continuous.

[0193] It should be noted here that when it comes to the description of orientation, the embodiment of the present invention is described based on the nucleic acid extraction device 100 being in a normal working state. In this normal working state, the nucleic acid extraction container 10 is vertically placed in the container receiving part of the fourth supporting member 121, and the axial direction of the nucleic acid extraction container 10 is the vertical direction.

[0194] As an embodiment, in order to ensure that the at least two magnets have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion when the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, it can be provided that: when the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, the at least two magnets in the mixing member 140 can move vertically relative to the fourth supporting member 121. However, this embodiment requires the addition of a mechanism for enabling the fourth supporting member 121 and the mixing member 140 to move vertically relative to each other, which increases the complexity and cost of the sample analyzer 1.

[0195] As another simpler embodiment, at least two magnets in the mixing member 140 have different heights relative to the fourth supporting member 121, that is, at least two magnets in the mixing member 140 are at different heights relative to the fourth supporting member 121. Preferably, the mixing member 140 and the fourth supporting member 121 do not move relative to each other in the vertical direction, and in particular, cannot move relative to each other in the vertical direction. This makes it easy to achieve that when the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, the at least two magnets are at different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0196] As an embodiment, the installation heights H of at least two magnets in the mixing member 140 on the magnet bracket 143 are different, that is, at least two magnets in the mixing member 140 are fixed on the magnet bracket 143 at different heights, so that at least two magnets in the mixing member 140 have different heights relative to the fourth supporting member 121.

[0197] It can be understood here that the installation height H can be the vertical distance between the bottom surface of the magnet and the bottom surface of the magnet bracket 143 when the magnet is installed on the magnet bracket 143.

[0198] As an embodiment, at least two magnets in the first magnet array 141 are installed at different heights H on the magnet support 143. Alternatively or additionally, at least two magnets in the second magnet array 142 are installed at different heights H on the magnet support 143.

[0199] As an embodiment, the magnet bracket 143 may be an integrally manufactured bracket, and the magnets in the first magnet array 141 and the magnets in the second magnet array 142 are both mounted on the integrally manufactured bracket.

[0200] As an embodiment, the magnet bracket 143 may include a first bracket and a second bracket, the magnets in the first magnet array 141 are mounted on the first bracket, and the magnets in the second magnet array 142 are mounted on the second bracket.

[0201] As an embodiment, the first magnet array 141 includes at least one first magnet unit 1411 and at least one second magnet unit 1421, each magnet unit including one magnet or multiple continuously distributed magnets. Each magnet in the first magnet unit 1411 (also referred to as the first lower magnet) is mounted on the magnet support 143 at a first height, and each magnet in the second magnet unit 1421 (also referred to as the first upper magnet) is mounted on the magnet support 143 at a second height greater than the first height. In other words, the first magnet array 141 includes at least two layers of magnets at different heights.

[0202] As an embodiment, the first magnet array 141 includes two first magnet units 1411 and one second magnet unit 1421, and each magnet unit includes two magnets. Of course, in specific applications, the arrangement of the first magnet 1411 is not limited.

[0203] As an embodiment, the second magnet array 142 includes at least one third magnet unit and at least one fourth magnet unit, each of which includes one magnet or multiple continuously distributed magnets. Each magnet in the third magnet unit (also referred to as the second lower magnet) is mounted on the magnet support 143 at a third height, and each magnet in the fourth magnet unit (also referred to as the second upper magnet) is mounted on the magnet support 143 at a fourth height greater than the third height. In other words, the second magnet array 142 includes at least two layers of magnets at different heights.

[0204] As an embodiment, the first magnet array 141 includes two first magnet units 1411 and one second magnet unit 1421, each of which includes two magnets. The second magnet array 142 includes two third magnet units and one fourth magnet unit, each of which includes two magnets.

[0205] As an embodiment, the first height is equal to the third height. Alternatively or additionally, the second height is equal to the fourth height.

[0206] As an implementation manner, the first height may not be equal to the third height, and the second height may not be equal to the fourth height.

[0207] As an embodiment, the first magnet units 1411 and the second magnet units 1421 are alternately arranged, especially sequentially alternately arranged, along the horizontal extension direction R to form a first magnet array 141. In this embodiment, the first magnet array 141 includes two layers of magnets at different heights.

[0208] As an embodiment, the third magnet units and the fourth magnet units are alternately arranged, especially alternately arranged in sequence, along the horizontal extension direction R to form the second magnet array 142. In this embodiment, the second magnet array 142 includes two layers of magnets at different heights.

[0209] As an embodiment, the first magnet array 141 may further include at least one fifth magnet unit, the fifth magnet unit including one magnet or multiple continuously distributed magnets. Each magnet in the fifth magnet unit is mounted on the magnet support 143 at a fifth height greater than the second height. Optionally, the second magnet array 142 may further include at least one sixth magnet unit, the sixth magnet unit including one magnet or multiple continuously distributed magnets. Each magnet in the sixth magnet unit is mounted on the magnet support 143 at a sixth height greater than the fourth height.

[0210] As an implementation manner, the fifth height is equal to the sixth height. In other embodiments, the fifth height may not be equal to the sixth height.

[0211] As an embodiment, the first magnet unit 1411, the second magnet unit 1421 and the fifth magnet unit are alternately arranged along the horizontal extension direction R, especially alternately arranged in sequence to form the first magnet array 141. In this embodiment, the first magnet array 141 includes three layers of magnets at different heights.

[0212] As an embodiment, the third magnet unit, the fourth magnet unit and the sixth magnet unit are alternately arranged, especially alternately arranged in sequence, along the horizontal extension direction R to form the second magnet array 142. In this embodiment, the second magnet array 142 includes three layers of magnets at different heights.

[0213] The above scheme forms a first magnet array 141 and a second magnet array 142 respectively by alternatingly arranging magnet units at different heights. When the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, the mixing member 140 can generate a magnetic field that uniformly alternates in the horizontal and vertical directions, so that the magnetic beads in the nucleic acid extraction container 10 placed in the uniformly alternating magnetic field are spatially distributed as uniformly as possible in the entire liquid of the nucleic acid extraction container 10 or the temporal movement trajectory of the magnetic beads is uniformly distributed throughout the entire liquid of the nucleic acid extraction container 10. In particular, the magnetic beads in the nucleic acid extraction container 10 placed in the changing magnetic field can move uniformly in the circumferential and axial directions, thereby achieving uniform and sufficient mixing of the magnetic beads.

[0214] As an embodiment, each magnet unit includes two magnets, but the present invention is not limited thereto. For example, each magnet unit may include only one magnet, or may include three, four or more magnets, which is not specifically limited.

[0215] As an implementation manner, the magnet units at different heights may also have different numbers of magnets, which is not specifically limited.

[0216] As an embodiment, the extension direction R of the first magnet array 141 may be a circumferential direction or a linear direction.

[0217] As an implementation manner, the number of magnets in the first magnet array 141 and the second magnet array 142 may be equal or different.

[0218] As an embodiment, the magnets in the first magnet array 141 and the second magnet array 142 are magnets of the same specifications. It should be noted that the magnets in the first magnet array 141 and the second magnet array 142 can also be configured as magnets of different specifications according to needs, such as different magnetic field strengths, different quantities, different sizes, etc., so as to adapt to mixing in different scenarios.

[0219] As an embodiment, adjacent magnets of the first magnet array 141 and the second magnet array 142 are staggered along the horizontal extension direction R. That is, the line connecting the center points of adjacent magnets of the first magnet array 141 and the second magnet array 142 is not perpendicular to the extension direction R. By staggering the magnets of the first magnet array 141 and the second magnet array 142 along the extension direction R, when the nucleic acid extraction container 10 is close to the magnets, the magnetic field strength and gradient generated there reach maximum value, which can rapidly pull the magnetic beads into motion, disturb the fluid, and promote spatial material exchange in the fluid. When the nucleic acid extraction container 10 is between the adjacent magnets, the magnetic field gradient generated there decreases sharply, the force on the magnetic beads is very weak, and the magnetic beads are quickly diffused in the spatial flow field due to the movement of the flow field, achieving sufficient contact between the magnetic beads and the nucleic acids in the fluid.

[0220] As an embodiment, the magnets in the first magnet array 141 are spaced apart by the same distance in the horizontal extension direction R. That is, adjacent magnets in the first magnet array 141 have the same distance in the horizontal extension direction R.

[0221] Alternatively or additionally, the magnets in the second magnet array 142 are spaced apart by the same distance in the horizontal extension direction R. That is, adjacent magnets in the second magnet array 142 have the same distance in the horizontal extension direction R.

[0222] As an embodiment, the magnets in the first magnet array 141 and the magnets in the second magnet array 142 are both distributed along a circumferential direction serving as an extension direction R, and the first magnet array 141 and the second magnet array 142 are concentrically arranged. Specifically, the plurality of first magnets 1411 are distributed in a circular array, the plurality of second magnets 1421 are distributed in a circular array, and the plurality of second magnets 1421 are distributed around the periphery of the plurality of first magnets 1411. In this embodiment, the extension direction R of the magnets is a circumferential direction. In other embodiments, the extension direction R of the magnets is a linear direction, and the magnets in the first magnet array 141 and the second magnet array 142 are both distributed along a linear direction serving as the extension direction R.

[0223] As an embodiment, the magnet support 143 and the fourth bearing member 121 can be configured as a disk, in which case each magnet array is a circular array. In another example, the magnet support 143 and the fourth bearing member 121 can be configured as a sector disk, in which case each magnet array is an arc array.

[0224] As an embodiment, the magnets in the first magnet array 141 have first poles facing the fourth supporting member 121 , and the magnets in the second magnet array 142 have second poles facing the fourth supporting member 121 , and the polarities of the first pole and the second pole are opposite.

[0225] In other embodiments, the magnetic poles on the same side of adjacent magnets in the first magnet array 141 have opposite polarities, and / or the magnetic poles on the same side of adjacent magnets in the second magnet array 142 have opposite polarities.

[0226] Next, some embodiments of driving the mixing member 140 and the fourth bearing member 121 to move horizontally relative to each other will be described.

[0227] As an embodiment, the second functional component 120 also includes a third driving member, and the mixing member 140 includes a movable magnet bracket 143 and a plurality of magnets fixed on the magnet bracket 143, wherein the third driving member is used to drive the magnet bracket 143 to move, so as to drive the plurality of magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth supporting member 121.

[0228] As an embodiment, the third driving member is configured to drive the magnet support 143 to move in the extension direction R, so that the mixing member 140 moves in the extension direction R relative to the fourth supporting member 121, thereby driving the magnetic beads in the nucleic acid extraction container 10 located between the first magnet array 141 and the second magnet array 142 to move.

[0229] Specifically, when the third driving member drives the magnet support 143 to move, the magnetic beads in the nucleic acid extraction container 10 can circulate along the circumferential and axial directions of the nucleic acid extraction container 10 .

[0230] For example, when the third driving member drives the magnet bracket 143 to move in the extension direction R, the nucleic acid extraction container 10 placed on the fourth supporting member 121 can pass through the various magnets of the first magnet array 141 and the second magnet array 142 in the extension direction R in sequence at least once, preferably multiple times, so that the nucleic acid extraction container 10 is placed in a changing, preferably alternating, magnetic field.

[0231] For another example, when the third driving member drives the magnet support 143 to move in the extension direction R, the nucleic acid extraction container 10 placed on the fourth supporting member 121 passes back and forth in the extension direction R through at least a portion of the magnets of the first magnet array 141 and the second magnet array 142, so that the nucleic acid extraction container 10 is placed in a changing, preferably alternating, magnetic field.

[0232] As an embodiment, the fourth carrying member 121 is configured as a rotatable first disk, the magnet holder 143 is configured as a rotatable second disk, and the first disk and the second disk are concentrically arranged.

[0233] Further preferably, the second disc is configured to rotate synchronously with the first disc and independently of the first disc. Specifically, the first disc can drive the second disc to rotate together, so that the rotation of the first disc does not affect the relative horizontal movement between the first disc and the second disc caused by the independent rotation of the second disc. In other words, the rotating shaft of the second disc is connected to the rotating shaft of the first disc. When the first disc rotates, it can drive the second disc to rotate synchronously. The second disc can also rotate independently. Therefore, when the position of the nucleic acid extraction container 10 changes due to the rotation of the first disc, the relative motion relationship between the nucleic acid extraction container 10 and the magnetic field can remain basically unchanged.

[0234] As an embodiment, the mixing member 140 includes a movable magnet bracket 143 and a plurality of magnets fixed on the magnet bracket 143, wherein the third driving member is used to drive the magnet bracket 143 to move, so as to drive the plurality of magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth supporting member 121, so that the magnetic beads in the nucleic acid extraction container 10 placed on the fourth supporting member 121 perform three-dimensional movement in the liquid in the nucleic acid extraction container 10 under the action of the moving magnet.

[0235] As an embodiment, the mixing member 140 is used to generate a magnetic field, and the third driving member is used to drive the fourth supporting member 121 and the mixing member 140 to move relative to each other, so that the mixing member 140 generates an alternating magnetic field relative to the nucleic acid extraction container 10 on the fourth supporting member 121, thereby enabling the magnetic beads in the nucleic acid extraction container 10 to perform three-dimensional movement in the liquid in the nucleic acid extraction container 10 under the action of the alternating magnetic field.

[0236] As an embodiment, the fourth supporting member 121 includes at least a lysis zone 1211, and the nucleic acid extraction container 10 undergoes a lysis and capture link in the lysis zone 1211. In this lysis and capture link, the cells in the sample of the nucleic acid extraction container 10 are lysed to release nucleic acids, and the released nucleic acids are captured by the magnetic beads in the nucleic acid extraction container 10. The mixing member 140 is used to generate a magnetic field that at least covers the lysis zone 1211, and the third driving member is used to drive the fourth supporting member 121 and the mixing member 140 to move relative to each other, so that the magnetic beads in the nucleic acid extraction container 10 in the lysis zone 1211 move so that the magnetic beads capture the released nucleic acids.

[0237] As an embodiment, the nucleic acid extraction device 100 is divided into an upper portion and a lower portion. The upper portion of the nucleic acid extraction device 100 is primarily used to perform functions such as injection of cleaning solution, magnetic bead separation, waste liquid aspiration and drainage, and injection of elution reagent. The lower portion of the nucleic acid extraction device 100 is divided into five areas: a lysis area 1211, a first washing area 1212, a second washing area 1213, a drying area 1213, and an elution area 1214, which respectively perform functions such as lysis, washing, drying, and elution. The nucleic acid extraction container 10 can be grasped and transferred up and down by a transfer assembly 130 and placed at different workstations to perform different functional actions.

[0238] In one embodiment, the transfer assembly 130 is a robotic arm. The functional components in the upper portion of the nucleic acid extraction device 100 are divided into different workstations to perform corresponding functions. The lower portion of the nucleic acid extraction device 100 is divided into different areas, each performing a different function. The robotic arm then cooperates with the robotic arm to grip and transfer the nucleic acid extraction container 10.

[0239] As an embodiment, the upper portion of the nucleic acid extraction device 100 is provided with four workstations, wherein the first workstation is used to adsorb magnetic beads, remove waste liquid, and inject cleaning liquid for the sample after lysis in the nucleic acid extraction container 10. The second workstation is used to remove waste liquid and inject cleaning liquid for the sample after cleaning and incubation in the first washing zone 1212 in the nucleic acid extraction container 10. The third workstation is used to remove waste liquid for the sample after cleaning and incubation in the second washing zone 1212 in the nucleic acid extraction container 10. The fourth workstation is used to inject elution reagent for the sample after drying and incubation in the drying zone 1213 in the nucleic acid extraction container 10.

[0240] In one embodiment, the transfer assembly 130 is used to: clamp the nucleic acid extraction container 10 from the second functional assembly 120 (i.e., the mixing and incubation module) below to the four workstations of the first functional assembly 110 above, and clamp the nucleic acid extraction container 10 from the four workstations of the first functional assembly 110 above to the second functional assembly 120 below for operation. In alternative embodiments, the transfer assembly 130 can also be used to transfer the nucleic acid extraction container 10 between different workstations of the first functional assembly 110 above and / or between different areas of the second functional assembly 120 below.

[0241] As an embodiment, as an embodiment, the amplification device 300 is mainly used to amplify the nucleic acid extracted by the nucleic acid extraction device 100, so as to significantly increase the amount of nucleic acid in a relatively short period of time. Among them, the amplification method that can be used is polymerase chain reaction (PCR), loop mediated isothermal amplification (LAMP), isothermal and chimeric primer initiated amplification (ICAN), nucleic acid sequence-dependent amplification (NASBA), strand displacement amplification (SDA), ligase chain reaction (LCR), and rolling circle amplification (RCA).

[0242] In one embodiment, detection device 400 is primarily used to detect amplified nucleic acids. For example, detection device 400 is used to perform fluorescence detection on amplified nucleic acids. During nucleic acid amplification and real-time fluorescence detection, the signal of each nucleic acid amplification cycle is read to obtain a fluorescence amplification curve. Based on the fluorescence amplification curve, detection device 400 obtains a qualitative result (negative or positive), or obtains a quantitative analysis result or the concentration of the analyte in the sample using a calibration curve.

[0243] As an embodiment, the sample analyzer 1 further includes a container providing device 500, which is used to provide a nucleic acid extraction container 10 and an amplification reaction container. The sample container, the nucleic acid extraction container 10 and the amplification reaction container are three different containers. Among them, the sample container is mainly used to load samples collected from patients, that is, the sample container is mainly used to provide a storage space for the sample. The nucleic acid extraction container 10 is mainly used to carry samples for nucleic acid extraction, that is, the nucleic acid extraction container 10 is mainly used to provide a nucleic acid extraction site for the sample. The amplification reaction container is mainly used to carry nucleic acid extract for amplification reaction, that is, the amplification reaction container is mainly used to provide an amplification reaction site for the nucleic acid extract. The pipetting device 700 is mainly used to realize the transfer of nucleic acid extract between the nucleic acid extraction container 10 and the amplification reaction container. The nucleic acid extraction container 10 and the amplification reaction container are two different consumables of the sample analyzer 1. In this way, the nucleic acid extraction container 10 can be designed into a shape that is convenient for nucleic acid extraction according to needs, and the amplification reaction container can be designed into a shape that is convenient for amplification reaction according to needs. The nucleic acid extraction container 10 and the amplification reaction container are used as consumables. Specifically, the nucleic acid extraction container 10 and the amplification reaction container are both disposable containers. After a nucleic acid extraction container 10 completes the nucleic acid extraction of a test item, it is discarded and recycled. After an amplification reaction container completes the amplification reaction and detection of a test item, it is discarded and recycled without the need for cleaning and recycling. This can save the container cleaning step in the detection process, thereby helping to improve the detection efficiency and avoid the problem of cross-contamination caused by unclean cleaning affecting the accuracy of the sample detection results. Each sample is tested on the sample analyzer 1, which consumes at least one nucleic acid extraction container 10 and at least one amplification reaction container. This embodiment provides the nucleic acid extraction container 10 through the second consumable supply device and provides the amplification reaction container through the first consumable supply device. In this way, the continuous detection needs of batch samples can be met, which helps to avoid the situation where the sample detection efficiency is affected by the lack of amplification nucleic acid extraction container 10 and reaction container.

[0244] In one embodiment, the container supply device 500 includes a first consumable supply device and a second consumable supply device. The first consumable supply device is used to provide at least an amplification reaction container. The second consumable supply device is used to provide at least a nucleic acid extraction container 10. The sample dispensing device 200 is used to aspirate at least a portion of the sample from the sample container and dispense it into the nucleic acid extraction container 10 provided by the second consumable supply device; the pipetting device 700 is used to aspirate at least a portion of the nucleic acid extraction solution from the nucleic acid extraction container 10 and dispense it into the amplification reaction container provided by the first consumable supply device.

[0245] As an embodiment, the first consumables supply device is used to store amplification reaction containers, that is, the first consumables supply device can buffer a certain amount of amplification reaction containers to meet the supply demand of batch amplification reaction containers.

[0246] In one embodiment, the first consumables supply device is further configured to allow an operator or an operating robot to place the amplification reaction container to facilitate loading of the amplification reaction container. This means that the storage position and loading position of the amplification reaction container are located at the same location on the sample analyzer 1. Of course, in specific applications, as an alternative embodiment, the storage position and loading position of the amplification reaction container on the sample analyzer 1 may also be located at two different locations.

[0247] As an embodiment, the second consumable supply device is used to store the nucleic acid extraction containers 10 , that is, the second consumable supply device can buffer a certain amount of nucleic acid extraction containers 10 to meet the supply demand of batch nucleic acid extraction containers 10 .

[0248] As an embodiment, the second consumables supply device is also used for an operator or an operating robot to place the nucleic acid extraction container 10 to enable loading of the nucleic acid extraction container 10. That is, the storage position and loading position of the nucleic acid extraction container 10 are located at the same position on the sample analyzer 1. Of course, in specific applications, as an alternative embodiment, the storage position and loading position of the nucleic acid extraction container 10 on the sample analyzer 1 can also be located in two different positions.

[0249] As an embodiment, the second consumables supply device is arranged below or above the first consumables supply device, that is, the first consumables supply device and the second consumables supply device are arranged in the vertical direction, and the first consumables supply device and the second consumables supply device are distributed in different spaces on the upper and lower floors. This is conducive to arranging the consumables supply device that occupies a larger space in a space on one floor, thereby ensuring that the sample analyzer 1 can cache a larger number of consumables, and reducing the horizontal size of the sample analyzer 1, thereby effectively reducing the space occupied by the sample analyzer 1 and improving the space utilization rate of the laboratory where the sample analyzer 1 is located. Of course, in specific applications, as an alternative embodiment, the first consumables supply device and the second consumables supply device can also be distributed in the horizontal direction.

[0250] As an embodiment, the sample analyzer 1 further includes a scheduling device 903 , which is at least used to schedule the nucleic acid extraction container 10 provided by the container providing device 500 to the nucleic acid extraction device 100 and to schedule the amplification reaction container provided by the container providing device 500 to the amplification device 300 .

[0251] As an embodiment, the scheduling device 903 includes a first scheduling mechanism and a second scheduling mechanism. The first scheduling mechanism is used to schedule the nucleic acid extraction container 10 provided by the second consumable supply device to the nucleic acid extraction device 100, and the second scheduling mechanism is used to schedule the amplification reaction container provided by the first consumable supply device to the amplification device 300.

[0252] As an embodiment, the sample analyzer 1 further includes a sample storage device 800, which is used to store at least sample containers containing samples. The sample dispensing device 200 is used to aspirate at least a portion of the sample from the sample container provided by the sample storage device 800 and dispense it into the nucleic acid extraction container 10 provided by the second consumable supply device. The sample storage device 800 can store a certain number of sample containers to meet the needs of continuous batch testing of samples.

[0253] As an embodiment, the sample storage device 800 is also used for an operator or an operating robot to place a sample container for loading the sample container. That is, the storage position and loading position of the sample container are located at the same location on the sample analyzer 1. Of course, in specific applications, as an alternative embodiment, the storage position and loading position of the sample container on the sample analyzer 1 can also be located in two different locations.

[0254] As an embodiment, the sample container is provided with an identification code, which is associated with at least the test item information of the sample in the sample container. The sample analyzer 1 further includes an information acquisition component, which is used to identify the identification code to obtain at least the test item information of the sample.

[0255] As an embodiment, the sample analyzer 1 further includes a reagent storage compartment 901 and a reagent dispensing device 902. The reagent storage compartment 901 is used to store reagents, and the reagent dispensing device 902 is used to dispense the reagents provided by the reagent storage device into the nucleic acid extraction container 10 and / or the amplification reaction container. In this embodiment, the reagent dispensing device 902 is used to dispense the reagents into the nucleic acid extraction container 10 and / or the amplification reaction container. Of course, in specific applications, as an alternative embodiment, the reagents can also be pre-stored in the nucleic acid extraction container 10 and / or the amplification reaction container. In this case, the sample analyzer 1 can also be provided without the reagent storage compartment 901 and the reagent dispensing device 902.

[0256] As an embodiment, the reagent storage chamber 901 is used to store first and second category reagents. The first category reagents are used to react with samples to obtain nucleic acid extracts, and the second category reagents are used to react with nucleic acid extracts for amplification. The reagent dispensing device 902 is used to dispense the first category reagents stored in the reagent storage chamber 901 into the nucleic acid extraction container 10, and to dispense the second category reagents stored in the reagent storage chamber 901 into the amplification reaction container. The first category reagents are primarily used for nucleic acid extraction and are also called extraction reagents. The second category reagents are primarily used for amplification reactions and are also called amplification reagents.

[0257] As an embodiment, the first type of reagents includes a lysis reagent, a magnetic bead reagent and an elution reagent.

[0258] As an embodiment, the reagent storage bin 901 is also used for an operator or an operating robot to place reagents for loading the reagents, that is, the reagent storage position and the loading position are located at the same location on the sample analyzer 1. Of course, in specific applications, as an alternative embodiment, the reagent storage position and the loading position on the sample analyzer 1 can also be located in two different locations.

[0259] In one embodiment, the reagent storage compartment 901 is used to store first-type reagent containers and second-type reagent containers. The first-type reagent containers are used to hold first-type reagents, and the second-type reagent containers are used to hold second-type reagents. The reagent dispensing device 902 is used to draw the first-type reagent from the first-type reagent container and dispense it into the nucleic acid extraction container 10, and to draw the second-type reagent from the second-type reagent container and dispense it into the amplification reaction container.

[0260] As an embodiment, the reagent storage compartment 901 includes a first reagent storage component and a second reagent storage component. The first reagent storage component is used to store the first type of reagent. The reagent dispensing device 902 is used to draw the first type of reagent from the first reagent storage component and inject it into the nucleic acid extraction container 10. The nucleic acid extraction device 100 is used to perform a nucleic acid extraction operation on the liquid in the nucleic acid extraction container 10 that contains at least the sample and the first type of reagent to obtain a nucleic acid extract solution; the second reagent storage component is used to store the second type of reagent. The reagent dispensing device 902 is used to draw the second type of reagent from the second reagent storage component and inject it into the amplification reaction container. The amplification device 300 is used to amplify the liquid in the amplification reaction container that is made of at least the nucleic acid extract solution and the second type of reagent to obtain a test solution.

[0261] As an embodiment, the sample analyzer 1 is an all-in-one molecular diagnostic device for detecting RNA or DNA through nucleic acid extraction.

[0262] As an embodiment, the nucleic acid extraction device 100 is divided into two major parts, an upper part and a lower part. Among them, the upper part of the nucleic acid extraction device 100 is mainly used to realize the functions of injection of cleaning liquid, magnetic adsorption, and waste liquid suction and discharge. The lower part of the nucleic acid extraction device 100 is mainly used to realize functions such as incubation and mixing. In this embodiment, the key functional modules are designed in an upper and lower layout, and the components with similar functions are integrated into a functional module design. For example, as an embodiment, the lower part completes the functions of lysis, incubation, mixing, nucleic acid capture, nucleic acid washing, and nucleic acid elution in nucleic acid extraction; while the upper part realizes the functions of liquid injection, magnetic separation, waste liquid suction and discharge, and then by designing one or more manipulator functional modules, the nucleic acid extraction container 10 is grabbed and transferred between the upper and lower functional components and various workstations, thereby completing the complete nucleic acid extraction function. In this embodiment, all workstations that require incubation are integrated into one functional component, and all workstations that require suction and injection are integrated into another functional component. The two functional components are then distributed up and down, and the nucleic acid extraction container 10 is transferred back and forth up and down. In this way, parts with similar functions are integrated into one functional component, which can reduce the difficulty of designing and manufacturing the nucleic acid extraction device 100 while reducing the horizontal area.

[0263] Example 2:

[0264] 1 to 12 and FIG. 19 , the sample analyzer 1 provided in this embodiment differs from that in the first embodiment primarily in the manner in which the fourth bearing member 121 of the second functional component 120 is disposed. Specifically, in the first embodiment, the fourth bearing member 121 of the second functional component 120 is movable relative to the frame assembly; whereas in this embodiment, the bearing member of the second functional component 120 is stationary relative to the frame assembly.

[0265] Specifically, the sample analyzer 1 provided in this embodiment includes a frame assembly, on which the nucleic acid extraction device 100 is mounted. The second functional component 120 includes a fourth load-bearing member 121, which is fixed to the frame assembly for carrying the nucleic acid extraction container 10 for incubation. The fourth load-bearing member 121 is formed with at least one first transfer station 1215 and multiple accommodating cavities, each of which is used to accommodate a nucleic acid extraction container 10. The first load-bearing member 111 is fixed to the frame assembly. The transfer assembly 130 is used at least to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the first load-bearing member 111. The transfer assembly 130 is configured to move in at least three dimensions. The fourth load-bearing member 121 is mounted on the frame assembly in a stationary manner relative to the frame assembly for carrying the nucleic acid extraction container 10 for incubation. The first functional assembly 110 includes a first supporting member 111 and a first pipetting member 118. The first supporting member 111 is mounted on the frame assembly in a stationary manner relative to the frame assembly to support the nucleic acid extraction container 10 and perform the first magnetic attraction and first liquid aspiration operations. The first pipetting member 118 is used to perform the first liquid aspiration operation on the nucleic acid extraction container 10 located on the first supporting member 111. The transfer assembly 130 is used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the first supporting member 111. In this embodiment, the supporting members of the first and second functional assemblies 110, 120, for supporting the nucleic acid extraction container 10 are both fixed relative to the frame assembly.

[0266] As an embodiment, the second functional component 120 is formed with at least two first transfer stations 1215; the first functional component 110 also includes a second supporting member 112, a second pipetting member 114, a third supporting member 113 and a third pipetting member 117. The second supporting member 112 and the third supporting member 113 are both installed on the frame assembly in a stationary manner relative to the frame assembly to carry the nucleic acid extraction container 10 to perform a first liquid aspiration action, the second pipetting member 114 is used to perform a second liquid aspiration action on the nucleic acid extraction container 10 located on the second supporting member 112 and after completing the first liquid aspiration action, and the third pipetting member 117 is used to perform a second liquid aspiration action on the nucleic acid extraction container 10 located on the third supporting member 113 and after completing the second liquid aspiration action. During the liquid injection action, the fourth supporting member 121 is formed with a lysis zone 1211, a washing zone 1212, a drying zone 1213 and an elution zone 1214. The number of first transfer stations 1215 is the same as the sum of the number of lysis zones 1211, the washing zone 1212, the drying zone 1213 and the elution zone 1214, and each lysis zone 1211, the washing zone 1212, the drying zone 1213 and the elution zone 1214 is respectively provided with a first transfer station 1215; the transfer assembly 130 is used to transfer the nucleic acid extraction container 10 between the first transfer stations 1215 corresponding to the lysis zone 1211, the washing zone 1212, the drying zone 1213 and the elution zone 1214 and the first supporting member 111, the second supporting member 112 and the third supporting member 113.

[0267] As an embodiment, the first functional component 110 includes two first bearing members 111, a second bearing member 112 and a third bearing member 113, the fourth bearing member 121 forms a cracking zone 1211, two washing zones 1212, a drying zone 1213 and an elution zone 1214, the number of first transfer stations 1215 is five, and each cracking zone 1211, washing zone 1212, drying zone 1213 and elution zone 1214 is respectively provided with a first transfer station 1215.

[0268] As an embodiment, the first supporting member 111, the second supporting member 112 and the third supporting member 113 are arranged side by side in sequence along the horizontal straight line direction, and the first transfer station 1215 corresponding to the cracking area 1211, the washing area 1212, the drying area 1213 and the elution area 1214 are distributed at intervals along the horizontal circumferential direction.

[0269] As an embodiment, the transfer assembly 130 includes a manipulator, which can drive the nucleic acid extraction container 10 to move to the first supporting member 111, the second supporting member 112, the third supporting member 113 and the first transfer stations 1215 corresponding to the lysis area 1211, the washing area 1212, the drying area 1213 and the elution area 1214 respectively.

[0270] In this embodiment, the supporting members of the first functional assembly 110 and the second functional assembly 120 for supporting the nucleic acid extraction container 10 are fixed relative to the frame assembly. The transfer assembly 130 is moved to achieve the transfer of the nucleic acid extraction container 10 between the first functional assembly 110 and the second functional assembly 120.

[0271] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first embodiment and will not be described in detail here.

[0272] Example 3:

[0273] The sample analyzer 1 provided in this embodiment differs from the second embodiment primarily in the arrangement of the supporting members of the first functional component 110. Specifically, in the second embodiment, the supporting members of the first functional component 110 are arranged side by side along a horizontal straight line; whereas in this embodiment, the supporting members of the first functional component 110 are arranged at intervals along a horizontal circumferential direction.

[0274] Specifically, in the sample analyzer 1 provided in this embodiment, at least two first transfer stations 1215 are arranged in sequence along the horizontal circumferential direction, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged in sequence along the horizontal circumferential direction, and the transfer assembly 130 includes a single first clamping member, a single second driving member and a single rotating driving member. The first clamping member is used to clamp and release the nucleic acid extraction container 10, and the rotating driving member is used to drive the first clamping member and the second driving member to rotate horizontally so that the first clamping member rotates to the same orientation as the first transfer station 1215, the first bearing member 111, the second bearing member 112, and the third bearing member 113. The second driving member is used to drive the first clamping member to perform two-dimensional linear motion so that the first clamping member moves to the first transfer station 1215, the first bearing member 111, the second bearing member 112 and the third bearing member 113 to clamp and release the nucleic acid extraction container 10. In this embodiment, the first, second, and third supporting members 111, 112, and 113 of the upper layer are spaced apart along the horizontal circumference. The first transfer stations 1215 of the lower layer, corresponding to the pyrolysis zone 1211, washing zone 1212, drying zone 1213, and elution zone 1214, are spaced apart along the horizontal circumference. The transfer assembly 130 rotates to direct the first clamping member toward each station.

[0275] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first and second embodiments and will not be described in detail here.

[0276] Example 4:

[0277] The sample analyzer 1 provided in this embodiment differs from the third embodiment mainly in that the setting method of the transfer component 130 is different, which is specifically reflected in that: in the third embodiment, the transfer component 130 includes a single first clamping member, a single second driving member and a single rotating driving member, and the first clamping member is respectively aligned with each first transfer station 1215 by rotation; while in this embodiment, the transfer component 130 is provided with a first clamping member corresponding to each first transfer station 1215, and the first clamping member can be aligned with the first transfer station 1215 without rotation.

[0278] Specifically, in the sample analyzer 1 provided in this embodiment, at least two first transfer stations 1215 are arranged in sequence along the horizontal circumferential direction, the first bearing member 111, the second bearing member 112, and the third bearing member 113 are arranged in sequence along the horizontal circumferential direction, and the transfer assembly 130 includes at least two first clamping members and at least two second driving members. The number of first clamping members and the number of second driving members are the same as the number of first transfer stations 1215. Each first clamping member is used to clamp and release the nucleic acid extraction container 10 between one of the first bearing member 111, the second bearing member 112, and the third bearing member 113 and a first transfer station 1215, and each second driving member is used to drive a first clamping member to perform two-dimensional linear motion so that the first clamping member moves to one of the first bearing member 111, the second bearing member 112, and the third bearing member 113 and a first transfer station 1215 to clamp and release the nucleic acid extraction container 10. In this embodiment, a first clamping member and a second driving member are provided corresponding to each first transfer station 1215, and the first clamping member does not need to rotate.

[0279] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to third embodiments and will not be described in detail here.

[0280] Embodiment 5:

[0281] The sample analyzer 1 provided in this embodiment differs from the first embodiment primarily in the manner in which the supporting member of the first functional component 110 is disposed. Specifically, in the first embodiment, the supporting member of the first functional component 110 is stationary relative to the frame assembly; whereas in this embodiment, the supporting member of the first functional component 110 is movable relative to the frame assembly.

[0282] Specifically, the sample analyzer 1 provided in this embodiment includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly; the second functional component 120 includes a fourth bearing member 121 and a first driving member 122, and the fourth bearing member 121 can be movably installed on the frame assembly to carry the nucleic acid extraction container 10 to perform an incubation action, and the fourth bearing member 121 is formed with at least one first transfer station 1215 and a plurality of accommodating cavities, each accommodating cavity is used to accommodate a nucleic acid extraction container 10, and the first driving member 122 is used to drive the fourth bearing member 121 to move relative to the frame assembly so that the fourth bearing member 121 drives the nucleic acid extraction container 10 to move to the first transfer station 1215 and the first driving member 122 respectively. Transfer station 1215; the first functional component 110 includes a first bearing member 111, a first pipetting member 118 and a second driving member. The first bearing member 111 can be movably mounted on the frame assembly to carry the nucleic acid extraction container 10 to perform a first liquid aspiration action. The second driving member is used to drive the first bearing member 111 to move relative to the frame assembly so that the first bearing member 111 drives the nucleic acid extraction container 10 to move to the second transfer station. The first pipetting member 118 is used to perform a first liquid aspiration action on the nucleic acid extraction container 10 located on the first bearing member 111. The transfer component 130 is used to transfer the nucleic acid extraction container 10 between the first transfer station 1215 and the second transfer station.

[0283] As an embodiment, the first functional component 110 also includes a second bearing member 112, a third bearing member 113, a fourth driving member and a fifth driving member. The fourth bearing member 121 is formed with a cracking area 1211, a washing area 1212, a drying area 1213 and an elution area 1214. The number of the first transfer station 1215 is one, and the number of the second transfer station is one. The second bearing member 112 and the third bearing member 113 are respectively movably mounted on the frame assembly. The fourth driving member is used to drive the second bearing member 112 to move relative to the frame assembly so that the second bearing member 112 drives the nucleic acid extraction container 10 to move to the second transfer station. The fifth driving member is used to drive the third bearing member 113 to move relative to the frame assembly so that the third bearing member 113 drives the nucleic acid extraction container 10 to move to the second transfer station.

[0284] As an embodiment, the first functional component 110 includes two first supporting members 111, a second supporting member 112 and a third supporting member 113, and the fourth supporting member 121 is formed with a cracking zone 1211, two washing zones 1212, a drying zone 1213 and an elution zone 1214.

[0285] As an embodiment, the first supporting member 111, the second supporting member 112 and the third supporting member 113 are arranged side by side in sequence along the horizontal straight line direction, and the first transfer station 1215 corresponding to the cracking area 1211, the washing area 1212, the drying area 1213 and the elution area 1214 are distributed at intervals along the horizontal circumferential direction.

[0286] As an embodiment, the transfer component 130 includes a robot, which can drive the nucleic acid extraction container 10 to move to the first transfer station 1215 and the second transfer station respectively.

[0287] In this embodiment, the supporting members of the first functional component 110 and the second functional component 120 for supporting the nucleic acid extraction container 10 are both movably arranged relative to the frame component.

[0288] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to fourth embodiments and will not be described in detail here.

[0289] Example 6:

[0290] The sample analyzer 1 provided in this embodiment differs from the first embodiment mainly in the different arrangements of the supporting members of the first functional component 110 and the second functional component 120. Specifically, in the first embodiment, the supporting member of the first functional component 110 is stationary relative to the frame assembly, while the supporting member of the second functional component 120 is movable relative to the frame assembly; whereas in this embodiment, the supporting member of the first functional component 110 is movable relative to the frame assembly, while the supporting member of the second functional component 120 is stationary relative to the frame assembly.

[0291] Specifically, the sample analyzer 1 provided in this embodiment includes a frame assembly, and the nucleic acid extraction device 100 is installed on the frame assembly; the second functional component 120 includes a fourth bearing member 121, and the fourth bearing member 121 is installed on the frame assembly in a stationary manner relative to the frame assembly for carrying the nucleic acid extraction container 10 to perform an incubation action; the first functional component 110 includes a first bearing member 111, a first pipetting member 118 and a second driving member. The first bearing member 111 can be movably installed on the frame assembly for carrying the nucleic acid extraction container 10 to perform a first liquid aspiration action, and the second driving member is used to drive the first bearing member 111 to move relative to the frame assembly so that the first bearing member 111 drives the nucleic acid extraction container 10 to move to the second transfer station, the first pipetting member 118 is used to perform a first liquid aspiration action on the nucleic acid extraction container 10 located on the first bearing member 111, and the transfer component 130 is used to transfer the nucleic acid extraction container 10 between the fourth bearing member 121 and the second transfer station. In this embodiment, the bearing member of the second functional component 120 located at the bottom is stationary, and the bearing member of the first functional component 110 located at the top is movable.

[0292] As an embodiment, the first functional component 110 further includes a second bearing member 112, a third bearing member 113, a fourth driving member and a fifth driving member. The fourth bearing member 121 is formed with a cracking area 1211, a washing area 1212, a drying area 1213 and an elution area 1214. The number of the second transfer station is one. The second bearing member 112 and the third bearing member 113 are respectively movably mounted on the frame assembly. The fourth driving member is used to drive the second bearing member 112 to move relative to the frame assembly so that the second bearing member 112 drives the nucleic acid extraction container 10 to move to the second transfer station. The fifth driving member is used to drive the third bearing member 113 to move relative to the frame assembly so that the third bearing member 113 drives the nucleic acid extraction container 10 to move to the second transfer station.

[0293] As an embodiment, the first functional component 110 includes two first supporting members 111, a second supporting member 112 and a third supporting member 113, and the fourth supporting member 121 is formed with a cracking zone 1211, two washing zones 1212, a drying zone 1213 and an elution zone 1214.

[0294] As an embodiment, the transfer component 130 includes a robot, which can respectively drive the nucleic acid extraction container 10 to move to the lysis area 1211, the washing area 1212, the drying area 1213, the elution area 1214 and the second transfer station.

[0295] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to fifth embodiments and will not be described in detail here.

[0296] Embodiment seven:

[0297] The sample analyzer 1 provided in this embodiment differs from that in the first embodiment mainly in that the functional components of the upper and lower parts of the nucleic acid extraction device 100 are defined differently. Specifically, the first embodiment defines that one of the upper and lower functional components of the nucleic acid extraction device 100 is used to at least perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container 10, and the other is used to at least incubate the liquid in the nucleic acid extraction container 10. However, the present embodiment does not define the specific functional actions of the upper and lower functional components of the nucleic acid extraction device 100. It only requires that the upper and lower parts be used to perform different functional actions or to perform the same functional action in sequence.

[0298] Specifically, the sample analyzer 1 provided in this embodiment includes a sample dispensing device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300 and a detection device 400. The sample dispensing device 200 is used to draw a sample from a sample container and dispense at least part of the drawn sample into the nucleic acid extraction container 10; the nucleic acid extraction device 100 is used to extract nucleic acid from a liquid containing at least a sample and a reagent in the nucleic acid extraction container 10 to obtain a nucleic acid extract; the pipetting device 700 is used to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container; the amplification device 300 is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; and the detection device 400 is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution. 400 is used to detect the test liquid; wherein, the nucleic acid extraction device 100 includes a first functional component 110, a second functional component 120 and a transfer component 130, the first functional component 110 is arranged above or below the second functional component 120, and the transfer component 130 is used to transfer the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120, the first functional component 110 is at least used to carry the nucleic acid extraction container 10 to perform a first functional action and the second functional component 120 is at least used to carry the nucleic acid extraction container 10 to perform a second functional action to perform nucleic acid extraction; the first functional action and the second functional action are different functional actions or the same functional action performed successively. In this embodiment, the first functional component 110 and the second functional component 120 can be: one is used to perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container 10, and the other is used to incubate the liquid in the nucleic acid extraction container 10; it may not necessarily be: one is used to perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container 10, and the other is used to incubate the liquid in the nucleic acid extraction container 10. For example, the first functional component 110 and the second functional component 120 can both integrate components with incubation, magnetic adsorption, and liquid aspiration functions.

[0299] As an embodiment, the first functional action includes at least one of a liquid aspiration action, a liquid injection action, and a magnetic adsorption action. Grouping parts with similar functions into one functional component helps reduce the difficulty of design and manufacturing.

[0300] As an embodiment, the second functional action includes at least one of an incubation action and a mixing action. Grouping parts with similar functions into one functional component helps reduce the difficulty of design and manufacturing.

[0301] As an embodiment, the second functional component 120 includes a fourth supporting member 121 and a mixing member 140, the fourth supporting member 121 is used to support the nucleic acid extraction container 10 to perform an incubation action, and the mixing member 140 is used to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the fourth supporting member 121; wherein the mixing member 140 includes at least a first magnet array 141 and a second magnet array 142 extending horizontally, each magnet array includes a plurality of magnets installed at intervals on a magnet bracket 143; the fourth supporting member 121 includes a container receiving portion for placing the nucleic acid extraction container 10; the fourth supporting member 121 and the mixing member 140 are arranged relative to each other The fourth supporting member 121 and the mixing member 140 are configured such that: when the nucleic acid extraction container 10 is received in the container receiving portion, at least the bottom of the nucleic acid extraction container 10 is located between the first magnet array 141 and the second magnet array 142; the fourth supporting member 121 and the mixing member 140 can move horizontally relative to each other, so that the mixing member 140 can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container 10 received in the container receiving portion; the fourth supporting member 121 and the mixing member 140 are also configured relative to each other: when the fourth supporting member 121 and the mixing member 140 move horizontally relative to each other, at least two magnets in the mixing member 140 have different heights relative to the bottom of the nucleic acid extraction container 10 received in the container receiving portion.

[0302] As an embodiment, the mixing member 140 is used to generate a magnetic field to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located on the fourth supporting member 121 .

[0303] As an embodiment, the second functional component 120 also includes a third driving member, the mixing member 140 includes a movable magnet bracket 143 and a plurality of magnets fixed on the magnet bracket 143, and the third driving member is used to drive the magnet bracket 143 to move, so as to drive the plurality of magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth supporting member 121.

[0304] As an embodiment, the third driving member is used to drive the magnet bracket 143 to drive the multiple magnets fixed on the magnet bracket 143 to move relative to the nucleic acid extraction container 10 on the fourth supporting member 121, mainly used to enable the magnetic beads in the nucleic acid extraction container 10 placed on the fourth supporting member 121 to perform three-dimensional movement in the liquid of the nucleic acid extraction container 10 under the action of the moving magnets.

[0305] As an embodiment, the second functional component 120 includes a fourth bearing member 121, a mixing member 140 and a third driving member. The fourth bearing member 121 is used to carry the nucleic acid extraction container 10 to perform an incubation action. The mixing member 140 is used to generate a magnetic field to mix the liquid containing at least a sample and a reagent in the nucleic acid extraction container 10 located on the fourth bearing member 121. The third driving member is used to drive the fourth bearing member 121 and the mixing member 140 to move relative to each other, so that the mixing member 140 generates an alternating magnetic field relative to the nucleic acid extraction container 10 on the fourth bearing member 121, thereby enabling the magnetic beads in the nucleic acid extraction container 10 to perform three-dimensional movement in the liquid in the nucleic acid extraction container 10 under the action of the alternating magnetic field. Alternatively,

[0306] As an embodiment, the second functional component 120 includes a fourth bearing member 121, a mixing member 140 and a third driving member. The fourth bearing member 121 is used to carry the nucleic acid extraction container 10 to perform an incubation action. The fourth bearing member 121 includes at least a lysis area 1211. The nucleic acid extraction container 10 undergoes a lysis and capture link in the lysis area 1211. In this lysis and capture link, the cells in the sample of the nucleic acid extraction container 10 are lysed to release nucleic acids, and the released nucleic acids are captured by the magnetic beads in the nucleic acid extraction container 10. The mixing member 140 is used to generate a magnetic field that at least covers the lysis area 1211 to at least mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container 10 located in the lysis area 1211. The third driving member is used to drive the fourth bearing member 121 and the mixing member 140 to move relative to each other, so that the magnetic beads in the nucleic acid extraction container 10 in the lysis area 1211 move so that the magnetic beads capture the released nucleic acids.

[0307] The sample analyzer 1 provided in this embodiment arranges one of the first functional component 110 and the second functional component 120 for performing different functional actions or for performing the same functional action successively in the nucleic acid extraction device 100 above or below the other, and transfers the nucleic acid extraction container 10 between the first functional component 110 and the second functional component 120 by the transfer component 130. That is, the first functional component 110 and the second functional component 120 for performing different functional actions on the liquid in the nucleic acid extraction container 10 or for performing the same functional action successively are arranged in an upper and lower direction in the vertical direction, and the nucleic acid extraction container 10 is transferred to the first functional component 110 and the second functional component 120 respectively by the transfer component 130. This arrangement scheme can make full use of the vertical space to arrange the different functional components of the nucleic acid extraction device 100, thereby reducing the horizontal space occupied by the nucleic acid extraction device 100, thereby facilitating the miniaturization design of the sample analyzer 1 and improving the space utilization of the laboratory where the sample analyzer 1 is located.

[0308] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to sixth embodiments and will not be described in detail here.

[0309] Embodiment 8:

[0310] The sample analyzer 1 provided in this embodiment differs from that in the first embodiment mainly in the different definitions of the upper and lower parts of the nucleic acid extraction device 100. Specifically, in the first embodiment, one of the upper and lower functional components of the nucleic acid extraction device 100 is used to at least perform magnetic adsorption and liquid aspiration on the liquid in the nucleic acid extraction container 10, and the other is used to at least incubate the liquid in the nucleic acid extraction container 10; whereas in this embodiment, the nucleic acid extraction device 100 is mainly defined as having two workstations arranged in an upper and lower manner.

[0311] Specifically, the sample analyzer 1 provided in this embodiment includes a sample distribution device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300 and a detection device 400. The sample distribution device 200 is used to aspirate a sample from a sample container and dispense at least a portion of the aspirated sample into the nucleic acid extraction container 10; the nucleic acid extraction device 100 is used to extract nucleic acid from a liquid containing at least a sample and a reagent in the nucleic acid extraction container 10 to obtain a nucleic acid extract; the pipetting device 700 is used to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container; the amplification device 300 is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test liquid; and the detection device 400 is used to detect the test liquid. The nucleic acid extraction device 100 has at least two workstations, each of which is used to carry the nucleic acid extraction container 10 to perform a processing action to extract nucleic acid. There are at least two workstations distributed vertically up and down, and the processing action includes at least one of an incubation action, a mixing action, a magnetic adsorption action, a liquid aspiration action, and a liquid injection action. In this embodiment, at least two workstations of the nucleic acid extraction device 100 are arranged in the vertical direction, which can also achieve the purpose of reducing the horizontal occupied area of ​​the nucleic acid extraction device 100.

[0312] As an embodiment, the nucleic acid extraction apparatus 100 extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least the sample and the reagent and performing post-lysis processing.

[0313] As an embodiment, the nucleic acid extraction device 100 has a first layer of space and a second layer of space, and the first layer of space is arranged above or below the second layer of space; the first layer of space is provided with an aspiration and injection station, a single aspiration station and a single injection station; the second layer of space is provided with a lysis incubation station, a cleaning incubation station, a drying incubation station and an elution incubation station; wherein, the lysis incubation station is used to carry the nucleic acid extraction container 10 to perform a lysis incubation action; the cleaning incubation station is used to carry the nucleic acid extraction container 10 to perform a cleaning incubation action; the drying incubation station is used to carry the nucleic acid extraction container 10 to perform a drying incubation action; the elution incubation station is used to carry the nucleic acid extraction container 10 to perform an elution incubation action; the aspiration and injection station is used to carry the nucleic acid extraction container 10 to perform a first aspiration action and a first injection action of injecting a cleaning liquid; the single aspiration station is used to carry the nucleic acid extraction container 10 to perform a second aspiration action; and the single injection station is used to carry the nucleic acid extraction container 10 to perform a second injection action of injecting an elution reagent.

[0314] The sample analyzer 1 provided in this embodiment arranges at least two workstations in the nucleic acid extraction device 100 to be distributed vertically up and down, and each workstation is respectively arranged to carry the nucleic acid extraction container 10 to perform at least one processing action among incubation, mixing, magnetic adsorption, liquid aspiration, and liquid injection. That is, the at least two workstations for carrying the nucleic acid extraction container 10 to perform the processing action are arranged vertically up and down. This arrangement scheme can make full use of the vertical space to arrange the multiple workstations of the nucleic acid extraction device 100, thereby reducing the horizontal space occupied by the nucleic acid extraction device 100, and further facilitating the miniaturized design of the sample analyzer 1 and improving the space utilization of the laboratory where the sample analyzer 1 is located.

[0315] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to seventh embodiments and will not be described in detail here.

[0316] Embodiment 9:

[0317] The sample analyzer 1 provided in this embodiment differs from that in the first embodiment primarily in the manner in which the functional components of the upper and lower parts of the nucleic acid extraction device 100 are divided. Specifically, in the first embodiment, the upper and lower parts of the nucleic acid extraction device 100 are divided based on functional similarity: the upper part is an integrated structure related to the aspiration and injection functions, and the lower part is an integrated structure related to incubation and mixing. In the present embodiment, the upper and lower parts of the nucleic acid extraction device 100 are divided based on the sequence of actions. After the nucleic acid extraction container 10 simultaneously completes the incubation and aspiration and injection actions at a station in the lower part, it is transferred to a station in the upper part to simultaneously complete the incubation and aspiration and injection actions.

[0318] Specifically, the sample analyzer 1 provided in this embodiment includes a sample dispensing device 200, a nucleic acid extraction device 100, a pipetting device 700, an amplification device 300 and a detection device 400. The sample dispensing device 200 is used to draw a sample from a sample container and dispense at least part of the drawn sample into the nucleic acid extraction container 10; the nucleic acid extraction device 100 is used to perform a nucleic acid extraction operation on a liquid containing at least a sample and a reagent in the nucleic acid extraction container 10 to obtain a nucleic acid extract; the pipetting device 700 is used to transfer the nucleic acid extract in the nucleic acid extraction container 10 to an amplification reaction container; the amplification device 300 is used to perform a nucleic acid extraction operation on the nucleic acid extraction container 10 to obtain a nucleic acid extract; The nucleic acid extract in the container is amplified to obtain the test liquid; the detection device 400 is used to detect the test liquid; wherein, the nucleic acid extraction device 100 includes a lysis module, a cleaning module, an elution module and a transfer component 130, and at least two of the lysis module, the cleaning module and the elution module are distributed in the vertical direction, the lysis module is used to carry the nucleic acid extraction container 10 for lysis operation; the cleaning module is used to carry the nucleic acid extraction container 10 for cleaning operation; the elution module is used to carry the nucleic acid extraction container 10 for elution operation; the transfer component 130 is used to transfer the nucleic acid extraction container 10 to the lysis module, the cleaning module and the elution module in sequence.

[0319] As an embodiment, the nucleic acid extraction apparatus 100 extracts nucleic acid from a liquid containing at least a sample and a reagent, including: lysing the liquid containing at least the sample and the reagent and performing post-lysis processing.

[0320] As an embodiment, the nucleic acid extraction device 100 also includes a drying module, which is arranged below or above at least one of the lysis module, the cleaning module, and the elution module. The drying module is used to carry the nucleic acid extraction container 10 for drying operations, and the transfer component 130 is used to transfer the nucleic acid extraction container 10 to the lysis module, the cleaning module, the drying module, and the elution module in sequence.

[0321] As an embodiment, the nucleic acid extraction device 100 can be divided into multiple functional modules according to the sequence of the nucleic acid extraction process. The nucleic acid extraction container 10 can be transferred to different functional modules in sequence without the need for reversing. For example, the cleaning module includes both the aspiration and injection components and the incubation and mixing components, while the elution module includes both the aspiration and injection components and the incubation and mixing components.

[0322] As an embodiment, the nucleic acid extraction device 100 also includes a drying module, which is arranged below or above at least one of the lysis module, the cleaning module, and the elution module. The drying module is used to carry the nucleic acid extraction container 10 for drying operations, and the transfer component 130 is used to transfer the nucleic acid extraction container 10 to the lysis module, the cleaning module, the drying module, and the elution module in sequence.

[0323] As an embodiment, the number of cleaning modules can be two or one or three or more. When there are two cleaning modules, the transfer component 130 is used to transfer the nucleic acid extraction container 10 to the lysis module, one cleaning module, another cleaning module, the drying module, and the elution module in sequence.

[0324] In Example 1, all incubation structures and mixing structures are integrated into one functional component; in this embodiment, all incubation structures can be divided into multiple sub-incubation blocks, and other corresponding functional components are set in each sub-incubation block, such as liquid suction and injection components, temperature control components, magnetic suction components, mixing components, etc.

[0325] The sample analyzer 1 provided in this embodiment arranges at least two of the lysis module, the cleaning module, and the elution module in the nucleic acid extraction device 100 to be arranged vertically in an upper and lower direction. That is, the functional modules for carrying the nucleic acid extraction container 10 to perform different functional actions in sequence are arranged vertically in an upper and lower direction. This arrangement scheme can make full use of the vertical space to arrange the multiple functional modules of the nucleic acid extraction device 100, thereby reducing the horizontal space occupied by the nucleic acid extraction device 100, and further facilitating the miniaturized design of the sample analyzer 1 and improving the space utilization of the laboratory where the sample analyzer 1 is located.

[0326] In addition to the above, other parts of the sample analyzer 1 provided in this embodiment can refer to the first to eighth embodiments and will not be described in detail here.

[0327] The above description is only a preferred embodiment of the present invention and does not limit the patent scope of the present invention. All equivalent structural transformations made by using the contents of the present invention description and drawings under the inventive concept of the present invention, or direct / indirect application in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A sample analyzer, comprising: A sample dispensing device configured to aspirate a sample from a sample container and dispense at least a portion of the aspirated sample into a nucleic acid extraction container; A nucleic acid extraction device configured to perform nucleic acid extraction on a liquid in the nucleic acid extraction container that contains at least a sample and a reagent to obtain a nucleic acid extract, wherein the reagent contains magnetic beads; A pipetting device configured to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container; An amplification device configured to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device configured to detect the test solution; Wherein, the nucleic acid extraction device includes a first functional component, a second functional component, and a transfer component. The first functional component is disposed above or below the second functional component. The transfer component is configured to transfer the nucleic acid extraction container between the first functional component and the second functional component to perform the nucleic acid extraction to obtain the nucleic acid extract. The first functional component is configured to at least perform magnetic adsorption and liquid aspiration actions on the liquid in the nucleic acid extraction container. The second functional component is at least configured to incubate the liquid in the nucleic acid extraction container.

2. The sample analyzer according to claim 1, wherein the first functional component includes a first carrier member, a first pipetting member, and a first magnetic attraction member. The first carrier member is configured to carry the nucleic acid extraction container. The first magnetic attraction member is configured to perform a first magnetic adsorption on the liquid in the nucleic acid extraction container located on the first carrier member that contains at least a sample and a reagent to adsorb the magnetic beads in the nucleic acid extraction container. The first pipetting member is configured to perform a first liquid aspiration action on the liquid in the nucleic acid extraction container located on the first carrier member that has been subjected to the first magnetic adsorption by the first magnetic attraction member. The transfer component is configured to transfer the nucleic acid extraction container between the first carrier member and the second functional component. The magnetic adsorption includes the first magnetic adsorption, and the liquid aspiration action includes the first liquid aspiration action.

3. The sample analyzer according to claim 2, wherein the first functional component further includes a liquid injection member configured to perform a first liquid injection action of injecting a cleaning solution into the nucleic acid extraction container located on the first carrier member.

4. The sample analyzer according to claim 3, wherein the first functional component further comprises a second carrier member, a second pipetting member, and a second magnetic attraction member. The second carrier member is configured to carry the nucleic acid extraction container. The second magnetic attraction member is configured to perform a second magnetic adsorption on a liquid containing at least a cleaning solution in the nucleic acid extraction container located on the second carrier member. The second pipetting member is configured to perform a second liquid suction action on the liquid in the nucleic acid extraction container located on the second carrier member and subjected to the second magnetic adsorption by the second magnetic attraction member. The transfer assembly is further configured to transfer the nucleic acid extraction container between the second carrier member and the second functional component. The magnetic adsorption further includes the second magnetic adsorption, and the liquid suction action further includes the second liquid suction action.

5. The sample analyzer according to claim 4, wherein the first functional component further comprises a third carrier member and a third pipetting member. The third carrier member is configured to carry the nucleic acid extraction container. The third pipetting member is configured to perform a second liquid injection action of injecting an elution reagent into the nucleic acid extraction container located on the third carrier member. The transfer assembly is further configured to transfer the nucleic acid extraction container between the third carrier member and the second functional component.

6. The sample analyzer according to claim 5, wherein the sample analyzer further comprises a first lifting member. The first lifting member is connected to the second magnetic attraction member, and the first lifting member is configured to drive the second magnetic attraction member to move up and down relative to the nucleic acid extraction container.

7. The sample analyzer according to claim 5, wherein the nucleic acid extraction container is formed with a reaction well, a first pipette tip well, and a second pipette tip well. The reaction well is configured to carry a sample and a reagent for nucleic acid extraction. The first pipette tip well is configured to carry a first pipette tip. The second pipette tip well is configured to carry a second pipette tip. The volume of the first pipette tip is larger than the volume of the second pipette tip. The first pipetting member includes a first pipette tip connection component and a first liquid suction / discharge power component. The first pipette tip connection component is configured to be detachably connected to the first pipette tip to suck liquid through the first pipette tip under the action of the power provided by the first liquid suction / discharge power component. The third pipetting member includes a second pipette tip connection component and a second liquid suction / discharge power component. The second pipette tip connection component is configured to be detachably connected to the second pipette tip to suck and inject liquid through the second pipette tip under the action of the power provided by the second liquid suction / discharge power component.

8. The sample analyzer according to claim 7, wherein the nucleic acid extraction container is further formed with a waste liquid well. The first pipetting member and the second pipetting member are further configured to discharge the liquid sucked from the reaction well to the waste liquid well.

9. The sample analyzer according to claim 3, wherein the number of the first carrier members is at least two, and at least one injection member and at least one first pipetting member are correspondingly arranged for each first carrier member.

10. The sample analyzer according to claim 5, wherein the first carrier member, the second carrier member, and the third carrier member are arranged side by side in the horizontal direction.

11. The sample analyzer according to claim 5, wherein the second functional component includes a fourth carrier member and a heating member. The fourth carrier member is configured to carry the nucleic acid extraction container to perform an incubation action, and the heating member is configured to heat a liquid containing at least a sample and a reagent in the nucleic acid extraction container located on the fourth carrier member. The fourth carrier member includes a lysis zone, a washing zone, a drying zone, and an elution zone. Accommodation cavities for placing the nucleic acid extraction container are respectively formed in the lysis zone, the washing zone, the drying zone, and the elution zone. The transfer assembly is configured to transfer the nucleic acid extraction container between the lysis zone, the washing zone and the first carrier member, between the washing zone, the drying zone and the second carrier member, and between the drying zone, the elution zone and the third carrier member.

12. The sample analyzer according to claim 11, wherein the fourth carrier member includes an incubation tray, and the heating member is configured to heat the nucleic acid extraction container on the incubation tray. The incubation tray forms the lysis zone, the washing zone, the drying zone, and the elution zone, and a first transfer station is formed on the incubation tray. The incubation tray is configured to respectively schedule the nucleic acid extraction containers located in the lysis zone, the washing zone, the drying zone, and the elution zone to the first transfer station, and the transfer assembly is configured to schedule the nucleic acid extraction container located at the first transfer station to the first functional component.

13. The sample analyzer according to claim 11, wherein the sample analyzer further includes a controller, and the controller is configured to: control the transfer assembly to schedule the nucleic acid extraction container after performing the incubation action in the lysis zone to the first carrier member; control the first pipetting member to perform the first liquid suction action on the liquid in the nucleic acid extraction container in the first carrier member and control the liquid injection member to perform the first liquid injection action on the nucleic acid extraction container in the first carrier member; control the transfer assembly to transfer the nucleic acid extraction container after performing the first liquid injection action to the washing zone to perform the incubation action; control the transfer assembly to transfer the nucleic acid extraction container after performing the incubation action in the washing zone to the second carrier member; control the second pipetting member to perform the second liquid suction action on the liquid in the nucleic acid extraction container in the second carrier member; control the transfer assembly to transfer the nucleic acid extraction container after performing the second liquid suction action to the drying zone to perform a drying action on the nucleic acid extraction container; control the transfer assembly to transfer the nucleic acid extraction container after performing the drying action in the drying zone to the third carrier member; control the third pipetting member to perform the second liquid injection action on the nucleic acid extraction container in the third carrier member; Control the transfer component to transfer the nucleic acid extraction container after performing the second liquid injection action to the elution area to perform an elution action.

14. The sample analyzer according to claim 11, wherein the position of the bottom of the accommodation cavity in the elution area is higher than the position of the bottom of the accommodation cavity in the lysis area, higher than the position of the bottom of the accommodation cavity in the washing area, and higher than the position of the bottom of the accommodation cavity in the drying area; and / or, The position of the bottom of the accommodation cavity in the lysis area is at the same height as the position of the bottom of the accommodation cavity in the washing area and the position of the bottom of the accommodation cavity in the drying area.

15. The sample analyzer according to any one of claims 1 to 14, wherein the second functional component includes a fourth carrier member, a heating member, and a mixing member. The fourth carrier member is used to carry the nucleic acid extraction container to perform an incubation action, and the heating member is used to heat the liquid containing at least the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member; the mixing member is used to mix the liquid containing at least the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member.

16. The sample analyzer according to claim 15, wherein the second functional component further includes a first driving member, and the first driving member is used to drive the fourth carrier member to move so that the fourth carrier member drives the nucleic acid extraction container to move; The mixing member includes a plurality of magnets, and the plurality of magnets are alternately distributed on opposite sides in the horizontal direction of the movement track of the nucleic acid extraction container.

17. The sample analyzer according to claim 15, wherein the mixing member at least includes a first magnet array and a second magnet array extending horizontally. Each magnet array includes a plurality of magnets spaced apart and mounted on a magnet bracket; The fourth carrier member includes a container receiving portion for placing the nucleic acid extraction container; The fourth carrier member and the mixing member are arranged relative to each other such that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array; The fourth carrier member and the mixing member can move horizontally relative to each other, so that the mixing member can mix the liquid containing at least the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion; The fourth carrier member and the mixing member are further arranged relative to each other such that when the fourth carrier member and the mixing member move horizontally relative to each other, at least two of the magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

18. The sample analyzer according to claim 17, wherein at least two of the magnets in the mixing member have different heights relative to the fourth carrier member; or, The mounting heights of at least two of the magnets in the mixing member on the magnet support are different, so that at least two of the magnets in the mixing member have different heights relative to the fourth bearing member.

19. The sample analyzer according to claim 15, wherein the second functional component further includes a third driving member, and the mixing member includes a movable magnet bracket and a plurality of magnets fixed on the magnet bracket, where The third driving member is used to drive the magnet support to move, so as to drive the multiple magnets fixed on the magnet support to move relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container placed on the fourth bearing member perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the moving magnets; Or, The second functional component further includes a third driving member, the mixing member is used to generate a magnetic field, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth bearing member, so that the magnetic beads in the nucleic acid extraction container can perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; or, The second functional component further includes a third driving member, the fourth bearing member at least includes a lysis zone, the nucleic acid extraction container undergoes a lysis capture step in the lysis zone, in which the cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by the magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field covering at least the lysis zone, and the third driving member is used to drive the fourth bearing member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container in the lysis zone move, so that the magnetic beads can capture the released nucleic acids.

20. The sample analyzer according to any one of claims 2 to 14, wherein the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is installed on the frame assembly; The second functional component includes a fourth bearing member and a first driving member. The fourth bearing member is movably installed on the frame assembly to carry out an incubation action for the nucleic acid extraction container, and the fourth bearing member is formed with at least one first transfer station and a plurality of accommodation cavities, and each accommodation cavity is used to accommodate one nucleic acid extraction container. The first driving member is used to drive the fourth bearing member to move relative to the frame assembly so that the fourth bearing member drives a plurality of nucleic acid extraction containers to move to the first transfer station respectively; the first bearing member is fixed on the frame assembly; the transfer assembly is at least used to transfer the nucleic acid extraction container between the first transfer station and the first bearing member.

21. The sample analyzer according to any one of claims 2 to 14, wherein the sample analyzer further includes a frame assembly, and the nucleic acid extraction device is installed on the frame assembly; The second functional component includes a fourth carrying member fixed to the frame assembly for carrying the nucleic acid extraction container to perform an incubation action, and at least one first transfer station and a plurality of accommodating cavities are formed in the fourth carrying member, and each accommodating cavity is used for accommodating one nucleic acid extraction container; the first carrying member is fixed to the frame assembly; the transfer component is at least used to transfer the nucleic acid extraction container between the first transfer station and the first carrying member, and the transfer component is configured to be capable of at least three-dimensional movement.

22. The sample analyzer according to any one of claims 1 to 14, wherein the sample analyzer further comprises: A container providing device for providing the nucleic acid extraction container and the amplification reaction container; A reagent storage bin for storing a first type of reagent and a second type of reagent, wherein the first type of reagent is used to react with a sample to obtain a nucleic acid extraction solution, and the second type of reagent is used to react with the nucleic acid extraction solution for amplification; A reagent dispensing device for dispensing the first type of reagent stored in the reagent storage bin into the nucleic acid extraction container, and for dispensing the second type of reagent stored in the reagent storage bin into the amplification reaction container; A scheduling device for at least scheduling the nucleic acid extraction container provided by the container providing device to the nucleic acid extraction device and scheduling the amplification reaction container provided by the container providing device to the amplification device.

23. A sample analyzer comprising: A sample dispensing device for sucking a sample from a sample container and dispensing at least a part of the sucked sample into a nucleic acid extraction container; A nucleic acid extraction device for performing nucleic acid extraction on a liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extraction solution; A pipetting device for transferring the nucleic acid extraction solution in the nucleic acid extraction container to an amplification reaction container; An amplification device for amplifying the nucleic acid extraction solution in the amplification reaction container to obtain a test solution; A detection device for detecting the test solution; Wherein, the nucleic acid extraction device includes a first functional component, a second functional component and a transfer component, the first functional component is arranged above or below the second functional component, the transfer component is used to transfer the nucleic acid extraction container between the first functional component and the second functional component, the first functional component is at least used to carry the nucleic acid extraction container to perform a first functional action and the second functional component is at least used to carry the nucleic acid extraction container to perform a second functional action for nucleic acid extraction; The first functional action and the second functional action are different functional actions or the same functional action executed successively.

24. The sample analyzer according to claim 23, wherein the first functional action includes at least one of a liquid suction action, a liquid injection action, and a magnetic adsorption action; and / or, The second functional action includes at least one of an incubation action and a mixing action.

25. The sample analyzer according to claim 23 or 24, wherein the second functional component includes a fourth carrier member and a mixing member, the fourth carrier member is configured to carry the nucleic acid extraction container to perform an incubation action, and the mixing member is configured to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member; Among them, The mixing member at least includes a first magnet array and a second magnet array extending horizontally, and each magnet array respectively includes a plurality of magnets spacedly mounted on a magnet bracket; The fourth carrier member includes a container receiving portion for placing the nucleic acid extraction container; The fourth carrier member and the mixing member are arranged relative to each other such that when the nucleic acid extraction container is received in the container receiving portion, at least the bottom of the nucleic acid extraction container is located between the first magnet array and the second magnet array; The fourth carrier member and the mixing member can move horizontally relative to each other, so that the mixing member can mix at least the liquid containing the sample and the magnetic beads in the nucleic acid extraction container received in the container receiving portion; The fourth carrier member and the mixing member are further arranged relative to each other such that when the fourth carrier member and the mixing member move horizontally relative to each other, at least two of the magnets in the mixing member have different heights relative to the bottom of the nucleic acid extraction container received in the container receiving portion.

26. The sample analyzer according to claim 23 or 24, wherein the second functional component comprises a fourth carrying member, a mixing member, and a third driving member. The fourth carrying member is configured to carry the nucleic acid extraction container to perform an incubation action. The mixing member is configured to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth carrying member. The mixing member includes a movable magnet holder and a plurality of magnets fixed on the magnet holder. The third driving member is configured to drive the magnet holder to move, so as to drive the plurality of magnets fixed on the magnet holder to move relative to the nucleic acid extraction container on the fourth carrying member, such that the magnetic beads in the nucleic acid extraction container placed on the fourth carrying member perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the moving magnets; Or, The second functional component includes a fourth carrier member, a mixing member, and a third driving member, the fourth carrier member is configured to carry the nucleic acid extraction container to perform an incubation action, the mixing member is configured to generate a magnetic field to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located on the fourth carrier member, and the third driving member is configured to drive the fourth carrier member and the mixing member to move relative to each other, so that the mixing member generates an alternating magnetic field relative to the nucleic acid extraction container on the fourth carrier member, thereby enabling the magnetic beads in the nucleic acid extraction container to perform three-dimensional movement in the liquid of the nucleic acid extraction container under the action of the alternating magnetic field; Or, The second functional component includes a fourth carrier member, a mixing member, and a third driving member. The fourth carrier member is used to carry the nucleic acid extraction container to perform an incubation action. The fourth carrier member includes at least a lysis zone. The nucleic acid extraction container undergoes a lysis capture step in the lysis zone. In this lysis capture step, cells in the sample of the nucleic acid extraction container are lysed to release nucleic acids, and the released nucleic acids are captured by magnetic beads in the nucleic acid extraction container. The mixing member is used to generate a magnetic field that at least covers the lysis zone to mix at least the liquid containing the sample and the reagent in the nucleic acid extraction container located in the lysis zone. The third driving member is used to drive the fourth carrier member and the mixing member to move relative to each other, so that the magnetic beads in the nucleic acid extraction container located in the lysis zone move, so that the magnetic beads can capture the released nucleic acids.

27. A sample analyzer, comprising: A sample dispensing device, which is used to aspirate a sample from a sample container and dispense at least a part of the aspirated sample into a nucleic acid extraction container; A nucleic acid extraction device, which is used to perform nucleic acid extraction on the liquid containing at least the sample and the reagent in the nucleic acid extraction container to obtain a nucleic acid extract; A pipetting device, which is used to transfer the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container; An amplification device, which is used to amplify the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device, which is used to detect the test solution; Wherein, the nucleic acid extraction device has at least two stations, each station is respectively used to carry the nucleic acid extraction container to perform a processing action for nucleic acid extraction, at least two of the stations are distributed vertically up and down, and the processing action includes at least one of an incubation action, a mixing action, a magnetic adsorption action, a liquid aspiration action, and a liquid injection action.

28. The sample analyzer according to claim 27, wherein the nucleic acid extraction device performs nucleic acid extraction on a liquid containing at least a sample and a reagent, including: The process of lysing the liquid containing at least the sample and the reagent and the subsequent processing after lysis.

29. The sample analyzer according to claim 27 or 28, wherein the nucleic acid extraction device has a first layer space and a second layer space, and the first layer space is arranged above or below the second layer space; An aspiration and injection liquid station, a single aspiration liquid station, and a single injection liquid station are arranged in the first layer space; A lysis incubation station, a washing incubation station, a drying incubation station, and an elution incubation station are arranged in the second layer space; Among them, The lysis incubation station is used to carry the nucleic acid extraction container to perform a lysis incubation action; The washing incubation station is used to carry the nucleic acid extraction container to perform a washing incubation action; The drying incubation station is used to carry the nucleic acid extraction container to perform a drying incubation action; The elution incubation station is used to carry the nucleic acid extraction container to perform an elution incubation action; The aspiration and injection liquid station is used to carry the nucleic acid extraction container to perform a first liquid aspiration action and a first liquid injection action of injecting a washing solution; The single aspiration liquid station is used to carry the nucleic acid extraction container to perform a second liquid aspiration action; The single-injection liquid station is used to carry the nucleic acid extraction container to perform the second liquid injection action of injecting the elution reagent.

30. A sample analyzer, comprising: A sample dispensing device for sucking a sample from a sample container and dispensing at least a part of the sucked sample into a nucleic acid extraction container; A nucleic acid extraction device for performing a nucleic acid extraction operation on a liquid containing at least a sample and a reagent in the nucleic acid extraction container to obtain a nucleic acid extract; A pipetting device for transferring the nucleic acid extract in the nucleic acid extraction container to an amplification reaction container; An amplification device for amplifying the nucleic acid extract in the amplification reaction container to obtain a test solution; A detection device for detecting the test solution; Wherein, the nucleic acid extraction device includes a lysis module, a cleaning module, an elution module and a transfer assembly. At least two of the lysis module, the cleaning module and the elution module are distributed in the vertical direction. The lysis module is used to carry the nucleic acid extraction container to perform a lysis operation; the cleaning module is used to carry the nucleic acid extraction container to perform a cleaning operation; the elution module is used to carry the nucleic acid extraction container to perform an elution operation; the transfer assembly is used to sequentially transfer the nucleic acid extraction container to the lysis module, the cleaning module and the elution module.

31. The sample analyzer according to claim 30, wherein the nucleic acid extraction device performs nucleic acid extraction on a liquid containing at least a sample and a reagent, including: The process of lysing a liquid containing at least a sample and a reagent and the subsequent processing after lysis.

32. The sample analyzer according to claim 30 or 31, wherein the nucleic acid extraction device further includes a drying module, the drying module is disposed below or above at least one of the lysis module, the cleaning module and the elution module, the drying module is used to carry the nucleic acid extraction container to perform a drying operation, and the transfer assembly is used to sequentially transfer the nucleic acid extraction container to the lysis module, the cleaning module, the drying module and the elution module.

Images