Piston-type sampling and implanting apparatus

Abstract

A piston-type sampling and implanting apparatus, comprising a support (16), and a piston-type sampling drilling device and a driving mechanism that are mounted on the support (16), wherein the piston-type sampling drilling device comprises a piston rod assembly and a piston sleeve assembly; the piston rod assembly comprises a solid piston rod (9) and a piston rod connecting handle (10) fixed on the upper end of the solid piston rod (9); the piston sleeve assembly comprises a piston sleeve (11), a piston sleeve gear (12), a piston sleeve bearing (13), a piston sleeve bearing sleeve (14), and a piston sleeve bearing lower handle (15); the piston sleeve (11), the piston sleeve gear (12), and the piston sleeve bearing lower handle (15) are fixed together to form a rotating assembly capable of synchronously rotating; the piston sleeve (11) penetrates through the rotating assembly in the axis direction, and piston-type sliding fit is formed between the solid piston rod (9) and the piston sleeve (11); the driving mechanism comprises a linear driving mechanism and a rotary driving mechanism; the linear driving mechanism cooperates with the piston rod connecting handle (10) so as to drive the solid piston rod (9) to move upward and downward reciprocatingly inside the piston sleeve (11); the rotary driving mechanism cooperates with the piston sleeve gear (12) so as to drive the piston sleeve (11) to rotate around the axis of the piston sleeve.

Description

Piston-type sampling and planting device Technical Field

[0001] The present disclosure belongs to the field of biological and medical detection technology, and in particular, relates to a piston-type sampling and implanting device for a tissue chip instrument. Background Art

[0002] Tissue microarrays are made by neatly arranging dozens, hundreds, or even more tissue samples on a blank wax block. They are widely used in drug screening, toxicology testing, and other fields. The tissue microarray production process involves sampling from donor tissue using a sampling and implantation device and then implanting it onto a blank recipient wax block.

[0003] Current sampling and implantation devices are completely separate, utilizing separate drilling and sampling devices. A drill is first used to drill a hole in the recipient wax block, followed by a sampler to extract a sample from the donor wax block. Finally, the extracted tissue sample is transferred into the hole in the recipient wax block. This method presents the problem of separate drilling and sampling processes, resulting in complex operation. Specifically, the completely separate sampling and implantation device consists of two independent components: one for drilling and the other for sampling and implantation. There is no physical connection between the two components. During tissue microarray fabrication, the drilling component first creates a hole in the recipient wax block, generating wax debris that requires cleaning with other tools. After drilling, the sampling component removes the tissue sample from the donor wax block. The hole location is then marked with a marker, and the sampling needle is aligned with the hole location. The tissue sample is then inserted into the pre-drilled hole to complete the implantation. This sampling and implantation device involves many separate steps, resulting in low sampling efficiency, cumbersome and complex operation, a high chip fabrication failure rate, and high production costs. Technical issues

[0004] The technical problem to be solved by the present disclosure is to provide a piston-type sampling and implanting device with simple process, low production cost and easy use, so as to improve the success rate of chip production, improve the efficiency of tissue chip production and reduce costs. Technical Solutions

[0005] In order to solve the above technical problems, the present disclosure provides a piston-type sampling and planting device, comprising: a bracket; a piston-type sampling drill and a driving mechanism installed on the bracket, wherein the piston-type sampling drill comprises a piston rod assembly and a piston sleeve assembly, wherein the piston rod assembly comprises a solid piston rod and a piston rod connecting handle fixed to the upper end of the solid piston rod, the piston sleeve assembly comprises a piston sleeve, a piston sleeve gear, a piston sleeve bearing, a piston sleeve bearing sleeve and a piston sleeve bearing lower handle, the piston sleeve, the piston sleeve gear and the piston sleeve bearing lower handle are fixed together to form a synchronously rotating rotating assembly, the piston sleeve passes through the rotating assembly along the axial direction, and a piston-type sliding fit is formed between the solid piston rod and the piston sleeve; the driving mechanism comprises a linear driving mechanism and a rotary driving mechanism, wherein the linear driving mechanism cooperates with the piston rod connecting handle to drive the solid piston rod to reciprocate up and down in the piston sleeve; the rotary driving mechanism cooperates with the piston sleeve gear to drive the piston sleeve to rotate around its own axis.

[0006] Compared with the prior art, the piston-type sampling and planting device disclosed in the present invention has the following beneficial effects:

[0007] 1) The drilling, sampling, and planting functions are integrated into one, eliminating the tedious steps of repeated positioning and sampling device replacement in traditional separate operations. The drilling, sampling, and planting functions can be achieved simply by changing the movement of the piston-type sampling drill.

[0008] 2) The piston design allows the solid piston rod and piston sleeve to move relative to each other, making sampling more flexible and convenient;

[0009] 3) The up and down reciprocating motion of the solid piston rod and the rotational motion of the piston sleeve are relatively independent and do not interfere with each other, making control easier;

[0010] 4) It greatly simplifies the operation process, reduces the difficulty of tissue chip production, and improves production efficiency and success rate.

[0011] Furthermore, the linear drive mechanism includes a piston rod motor, a screw rod, a screw rod connecting block, a piston rod connecting block, a slide rail, a slider and a piston rod connecting block buffer spring. The piston rod motor and the slide rail are fixed on the bracket, the screw rod is connected to the output end of the piston rod motor, the screw rod connecting block forms a threaded fit with the screw rod, the screw rod connecting block is connected to the bottom of the piston rod connecting block, the piston rod connecting block buffer spring is arranged between the bottom of the piston rod motor and the piston rod connecting block, the piston rod connecting handle is fixed on the piston rod connecting block, the back of the piston rod connecting block is fixedly connected to the slider, and the slider forms a sliding fit with the slide rail.

[0012] The linear drive mechanism using the above structure, the piston rod motor, the lead screw, etc. can accurately control the up and down movement of the solid piston rod to ensure accurate positioning of sampling and planting; the linear motion structure using the slide rail and the slider ensures stable and accurate movement.

[0013] Among them, a piston rod connecting block is provided with a piston rod connecting handle slot for accommodating the piston rod connecting handle, and the piston rod connecting handle is embedded and fixed in the piston rod connecting handle slot.

[0014] Providing the piston rod connecting handle slot can better fix the piston rod connecting handle and avoid movement deviation, and the overall movement of the piston rod connecting handle and the piston rod connecting block is more precise.

[0015] Furthermore, the rotary drive mechanism includes a piston sleeve motor, a piston sleeve motor mounting frame, a crank, and a piston sleeve rack. The piston sleeve motor is installed on the bracket through the piston sleeve motor mounting frame. The output end of the piston sleeve motor is connected to the crank. One end of the piston sleeve rack is provided with a strip hole that cooperates with the crank pin on the crank, and the other end of the piston sleeve rack is engaged with the piston sleeve gear.

[0016] The motor is used to drive the crank mechanism to make the piston sleeve rotate, the speed is controllable and the structure is simple; moreover, the piston sleeve has a fast speed and high drilling efficiency.

[0017] Furthermore, it also includes a device translation mechanism for driving the bracket to perform translational movement, the bracket is installed on the device translation mechanism, and the device translation mechanism is fixed on the frame.

[0018] Since the device translation mechanism is set up, the entire device can be driven to move in the direction of the XYZ axis, which expands the working range and is more conducive to transporting the sampling device between different wax blocks; moreover, there is no need to manually move the sampling device, which simplifies the operation process.

[0019] Furthermore, the linear drive mechanism can also be a motor-driven synchronous belt mechanism or a handwheel-driven screw mechanism. A motor-driven synchronous belt mechanism offers precise movement and quick response, enabling accurate sampling. A motor-driven mechanism offers controllable speed and a high degree of automation. The synchronous belt mechanism has a simple structure and is easy to install and debug. A handwheel-driven screw mechanism offers flexible and convenient handwheel drive, requires no power supply, and has a simple structure and low cost. The screw mechanism offers high drive precision and stable and controllable sampling. Manual operation is simple, requiring no programming, and offers flexibility. The mechanism retains both automatic and manual operation modes, allowing for a wide range of applications.

[0020] Furthermore, a piston sleeve bearing sleeve slot is provided at the bottom of the bracket for accommodating and fixing the piston sleeve bearing sleeve, the piston sleeve bearing sleeve is fixed in the piston sleeve bearing sleeve slot, and the piston sleeve gear is arranged in the piston sleeve bearing sleeve slot.

[0021] Since the piston sleeve bearing sleeve clamping groove is provided, the position of the piston sleeve bearing sleeve can be better fixed; the piston sleeve gear is arranged in the clamping groove and can rotate freely to drive the piston sleeve to rotate.

[0022] Furthermore, the piston sleeve bearing includes an upper bearing and a lower bearing.

[0023] Since the piston sleeve bearing is arranged as two upper and lower bearings, the rotation of the piston sleeve can be better supported, making the rotation of the piston sleeve more stable.

[0024] Furthermore, an airtight piston gap is formed between the solid piston rod and the piston sleeve.

[0025] By setting the airtight piston gap, the sample can be prevented from falling off and being contaminated during the sampling process, thus ensuring the integrity of the sample during the sampling and planting process.

[0026] Furthermore, the lower end of the piston sleeve is processed to form an annular cutter.

[0027] By arranging the annular knife, the cutting resistance during the drilling process can be reduced and the sampling success rate can be improved; the annular knife rotates for drilling, and the drilling efficiency is higher.

[0028] Furthermore, the piston sleeve bearing lower handle is composed of a cylindrical handle body and a limiting flange at the lower end of the handle body, the piston sleeve gear is sleeved on the upper end of the handle body, the piston sleeve bearing is sleeved on the lower part of the handle body, and the piston sleeve bearing is fixed in the piston sleeve bearing.

[0029] The above-mentioned structure of the piston sleeve bearing lower handle can better fix and support the piston sleeve gear, which is beneficial to the rotation stability of the piston sleeve. Beneficial effects

[0030] In general, compared with the existing technology, the present invention has the advantages of simple structure, high integration, flexible operation, simple manufacturing process, low cost, and wide application range. It can effectively solve the problems existing in existing sampling and implantation equipment, greatly improve the efficiency of tissue chip production, reduce the difficulty and cost of operation, and is an important progress in the technological development of this field. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] FIG1 is a diagram showing a piston-type sampling and planting device in use according to a first embodiment of the present disclosure.

[0032] FIG2 is a perspective view of the piston-type sampling and planting device shown in FIG1 .

[0033] FIG3 is a partial cross-sectional view of the piston-type sampling and planting device shown in FIG2 .

[0034] FIG4 is an exploded view of the piston-type sampling and planting device shown in FIG2.

[0035] FIG5 is a perspective view of the piston-type sampling drill in the piston-type sampling and planting device shown in FIG2.

[0036] FIG6 is a cross-sectional view of the piston-type sampling drill shown in FIG5.

[0037] FIG. 7 is an exploded view of the piston-type sampling drill shown in FIG. 6 .

[0038] FIG8 is a diagram showing a piston-type sampling and planting device in use according to a second embodiment of the present disclosure.

[0039] FIG9 is a perspective view of the piston-type sampling and planting device shown in FIG8 .

[0040] FIG10 is a partial cross-sectional view of the piston-type sampling and planting device shown in FIG9 .

[0041] FIG11 is a diagram showing a piston-type sampling and planting device in use according to a third embodiment of the present disclosure.

[0042] FIG12 is a perspective view of the piston-type sampling and planting device shown in FIG11.

[0043] FIG13 is an exploded view of the piston-type sampling and planting device shown in FIG12.

[0044] List of reference numerals:

[0045] 1: Piston-type sampling and implantation device; 2: Tissue chip instrument; 3: Workbench; 4: Carrier; 5: Donor wax block; 6: Acceptor wax block; 7: Rack; 8: Device translation mechanism; 9: Solid piston rod; 10: Piston rod connecting handle; 11: Piston sleeve; 12: Piston sleeve gear; 13: Piston sleeve bearing; 14: Piston sleeve bearing sleeve; 15: Piston sleeve bearing lower handle; 16: Bracket; 17: Piston rod motor; 18: Screw; 19 : Screw connecting block; 20: Piston rod connecting block; 21: Slide rail; 22: Slider; 23: Piston rod connecting block buffer spring; 24: Piston sleeve motor; 25: Piston sleeve motor mounting bracket; 26: Crank; 27: Piston sleeve rack; 28: Strip hole; 29: Bracket; 30: Crank pin; 31: Piston rod connecting handle slot; 32: Piston sleeve bearing sleeve slot; 33: Synchronous belt motor; 34: Synchronous belt mechanism; 35: Handwheel. Implementation of the present disclosure

[0046] The specific embodiments of the present disclosure are described in detail below with reference to the accompanying drawings.

[0047] Example 1: As shown in Figure 1, the piston-type sampling and implantation device 1 of the present disclosure is used in conjunction with an automatic tissue chip instrument 2, the overall structure of which is shown in Figure 1. The structural composition and operating principle of the tissue chip instrument 2 can be referred to the description in the patent application "A Novel Fully Automatic Tissue Chip Instrument" with publication number CN113759143A. It includes a worktable 3 that can be adjusted and moved by an electrically controlled translation mechanism. The worktable 3 is provided with a plurality of carriers 4 arranged in a matrix, each carrier 4 being fixed with a donor wax block 5 or a recipient wax block 6.

[0048] The entire piston-type sampling and implanting device 1 is installed on a device translation mechanism 8 fixed to a frame 7. The device translation mechanism 8 drives the piston-type sampling and implanting device 1 through a motor and a slide rail mechanism to realize the horizontal, longitudinal and vertical translation movement of the piston-type sampling and implanting device 1 relative to the tissue chip instrument 2.

[0049] As shown in Figures 2 to 7, the piston-type sampling and planting device 1 primarily comprises a bracket 16, a piston-type sampling drill mounted on the bracket 16, and a drive mechanism. The piston-type sampling drill comprises a piston rod assembly and a piston sleeve assembly. The piston rod assembly includes a solid piston rod 9 and a piston rod connecting handle 10 fixed to the upper end of the solid piston rod 9. The piston sleeve assembly comprises a piston sleeve 11, a piston sleeve gear 12, a piston sleeve bearing 13, a piston sleeve bearing sleeve 14, and a piston sleeve bearing lower handle 15. The piston sleeve 11, piston sleeve gear 12, and piston sleeve bearing lower handle 15 are fixed together to form a synchronously rotating assembly. The piston sleeve 11 axially extends through the rotating assembly formed by the piston sleeve gear 12 and the piston sleeve bearing lower handle 15. The piston sleeve bearing lower handle 15 consists of a cylindrical handle body and a retaining flange at its lower end. The piston sleeve gear 12 fits over the upper end of the handle body, the piston sleeve bearing 13 fits over the lower end of the handle body, and the piston sleeve bearing 13 is fixed within the piston sleeve bearing sleeve 14. In this embodiment, the piston sleeve bearing 13 comprises an upper bearing and a lower bearing. In other embodiments, the piston sleeve bearing 13 can be a single bearing or comprised of multiple bearings. An annular cutter is formed at the lower end of the piston sleeve 11. The solid piston rod 9 and the piston sleeve 11 form a piston-like sliding fit, preferably forming an airtight piston gap.

[0050] The drive mechanism includes a linear drive mechanism for driving the solid piston rod 1 to reciprocate up and down within the piston sleeve 11, and a rotary drive mechanism for rotating the piston sleeve 11. The linear drive mechanism cooperates with the piston rod connecting handle 10 to drive the solid piston rod 9 fixed thereto to reciprocate up and down within the piston sleeve 11. The rotary drive mechanism cooperates with the piston sleeve gear 12 to drive the piston sleeve bearing lower handle 15 fixed thereto and the piston sleeve 11 to rotate about its own axis. These two motions are relatively independent and can be coordinated.

[0051] The linear drive mechanism includes a piston rod motor 17, a screw rod 18, a screw rod connecting block 19, a piston rod connecting block 20, a slide rail 21, a slider 22, and a piston rod connecting block buffer spring 23. The piston rod motor 17 and the slide rail 21 are fixed to the bracket 16. The screw rod 18 is connected to the output end of the piston rod motor 17. The screw rod connecting block 19 is threadedly engaged with the screw rod 18 and connected to the bottom of the piston rod connecting block 20. The piston rod connecting block buffer spring 23 is compressed and disposed between the bottom of the piston rod motor 17 and the piston rod connecting block 20. The piston rod connecting handle 10 is fixed to the piston rod connecting block 20. The back of the piston rod connecting block 20 is fixedly connected to the slider 22, which slides with the slide rail 21. The piston rod connecting block 20 is provided with a piston rod connecting handle slot 31 for accommodating the piston rod connecting handle 10. The piston rod connecting handle 10 is inserted and fixed in the piston rod connecting handle slot 31 and moves up and down synchronously with the piston rod connecting block 20.

[0052] The rotary drive mechanism includes a piston sleeve motor 24, a piston sleeve motor mounting bracket 25, a crank 26, and a piston sleeve rack 27. The piston sleeve motor 24 is mounted on the bracket 16 via the piston sleeve motor mounting bracket 25 and a bracket 29. The output end of the piston sleeve motor 24 is connected to the crank 26. One end of the piston sleeve rack 27 is provided with a strip-shaped hole 28 that mates with a crank pin 30 on the crank 26. The other end of the piston sleeve rack 27 meshes with the piston sleeve gear 12. In this way, the rotational motion output by the piston sleeve motor 24 is converted into translational motion of the piston sleeve rack 27 through the engagement of the crank pin 30 with the strip-shaped hole 28. This is then further converted into rotational motion of the piston sleeve 11 through the meshing of the piston sleeve rack 27 with the piston sleeve gear 12. A piston sleeve bearing sleeve slot 32 is provided at the bottom of the bracket 16 for accommodating and fixing the piston sleeve bearing sleeve 14 . The piston sleeve bearing sleeve 14 is fixed in the piston sleeve bearing sleeve slot 32 , and the piston sleeve gear 12 can rotate freely in the piston sleeve bearing sleeve slot 32 .

[0053] Example 2: As shown in Figures 8 to 10, the difference between the piston-type sampling and planting device of this embodiment and that of Example 1 mainly lies in the linear drive mechanism. In this embodiment, the linear drive mechanism uses a synchronous belt motor 33 to drive a synchronous belt mechanism 34, and then drives the piston rod connecting block 20 to perform lifting movements through the synchronous belt mechanism 34. The other structures are basically the same as those of Example 1 and will not be repeated here.

[0054] Example 3: As shown in Figures 11 to 13, the difference between the piston-type sampling and planting device of this embodiment and that of Example 1 is that all motors are replaced by hand wheels 35 and driven manually. The other structures are basically the same as those of Example 1 and will not be repeated here.

[0055] The piston-type sampling and implanting device disclosed herein can be installed on an automated or manually operated tissue chip instrument. The following describes the working process of the piston-type sampling and implanting device using the automated operation mode as an example:

[0056] 1) Install the entire piston-type sampling and implanting device 1 on the device translation mechanism 8 of the frame 7 of the automatic tissue chip instrument 2. The piston sleeve bearing sleeve 14 is fixed in the piston sleeve bearing sleeve slot 32, and the piston rod handle 10 is fixed in the piston rod handle slot 31;

[0057] 2) Design the tissue information for each chip point on the tissue microarray in the tissue microarray printing software according to the experimental design requirements;

[0058] 3) According to the experimental design requirements, the recipient wax block 6 and the donor wax block 5 are mounted on the automatic tissue chip instrument workbench 3 corresponding to the position 2;

[0059] 4) Set the chip production parameters such as the coordinate information of each sampling point and the hole spacing in the tissue chip printing software;

[0060] 5) The software sends a control signal, and the driving device translation mechanism 8 drives the entire piston-type sampling and planting device 1 to move to a predetermined position above the receptor wax block 6;

[0061] 6) Software controls the piston rod motor 17 and piston sleeve motor 24 to start. The piston rod motor 17 drives the piston rod connecting block 20 to move the solid piston rod 9 downward. Simultaneously, the piston sleeve motor 24 drives the piston sleeve 11 to rotate at high speed to align with the position of the receptor wax block 6 to drill a prefabricated hole.

[0062] 7) The software controls the piston rod motor 17 to move in the reverse direction, driving the solid piston rod 9 upward to push the wax core into the piston sleeve 11;

[0063] 8) The software control device translation mechanism 8 moves the piston-type sampling planting device 1 to the top of the waste wax collection box;

[0064] 9) The software controls the piston rod motor 17 to rotate forward, driving the solid piston rod 9 to move downward to discharge the waste wax core into the collection box;

[0065] 10) Repeat steps 4)-6), control the sampling device to sample the donor wax block 5 to obtain a tissue sample;

[0066] 11) Control piston type sampling planting device 1 is moved to the receptor wax block 6 to be planted position;

[0067] 12) Control the solid piston rod 9 to drop the tissue sample into the preformed hole of the receptor wax block 6;

[0068] 13) Repeat the above steps to complete the automatic drilling sampling and planting of all sampling points.

[0069] In summary, the piston-type sampling and planting device disclosed herein integrates drilling, sampling, and planting functions in one step, eliminating the tedious steps of repeated positioning and sampler replacement required in traditional separate operations. Simply by changing the device's motion, all drilling, sampling, and planting functions can be achieved without the need for additional structures, significantly reducing manufacturing processes and costs. Compared to existing technologies, the technical advantages of the present disclosure are primarily reflected in the following aspects:

[0070] 1. It adopts piston design, with solid piston rod moving in piston sleeve, which has simple and exquisite structure and improves accuracy;

[0071] 2. The piston sleeve and the solid piston rod can move relative to each other. Through different movement combinations, multiple functions can be achieved, making sampling more flexible and convenient;

[0072] 3. The sliding seal piston structure is adopted to facilitate automatic cleaning of the piston sleeve, avoiding damage and contamination of tissue samples during transfer;

[0073] 4. The up and down reciprocating motion of the solid piston rod and the rotational motion of the piston sleeve are relatively independent. They can move in coordination or separately without interfering with each other. This makes the control simpler, the use more flexible, and reduces the manufacturing difficulty.

[0074] 5. An annular cutter is provided at the lower end of the piston sleeve to reduce cutting resistance and improve drilling success rate;

[0075] 6. Simple structure, motion and control mode integrate all functions of tissue chip production, greatly reducing process and production costs and greatly simplifying the operation process;

[0076] 7. Automatic switching among drilling, sampling and planting can be realized without adding other structures, and the structure and production are relatively simple;

[0077] 8. The device is simple to operate, highly flexible, and offers diverse motion patterns. It is suitable for both manual and software-controlled automated operations. The manual operation mode is simple and easy, while the automatic control mode enables high-throughput chip production. It can be widely used in the production of various in vitro tissue chips and has broad application prospects and market potential.

[0078] 9. Greatly simplifies the operation process, reduces the difficulty of tissue chip production, and significantly improves the efficiency and success rate of tissue chip production;

[0079] 10. No additional automation structure is required, and the waste wax generated by punching can be automatically cleaned to simplify the operation.

[0080] The above describes the differences between the present disclosure and the prior art, as well as the technical advantages of the present disclosure. Overall, the present disclosure offers advantages such as simple structure, high integration, flexible operation, simplified manufacturing process, low cost, and a wide range of applications. It effectively addresses the issues existing sampling and implantation equipment, significantly improving the efficiency of tissue chip production and representing a significant advancement in this field.

[0081] The present disclosure is described above through embodiments, but the protection scope of the present disclosure is not limited to the above embodiments. All variations that are directly or indirectly realized by utilizing the concepts and principles of the present disclosure should be included in the protection scope of the present disclosure. Industrial Applicability

[0082] The piston-type sampling and planting device disclosed herein can be manufactured and used industrially, and therefore has industrial applicability.

Claims

1. Piston sampling and planting device, comprising: Bracket (16); A piston sampling drill and a driving mechanism mounted on the bracket (16), wherein The piston-type sampling drill includes a piston rod assembly and a piston sleeve assembly, where, The piston rod assembly includes a solid piston rod (9) and a piston rod connecting handle (10) fixed to the upper end of the solid piston rod (9). The piston sleeve assembly includes a piston sleeve (11), a piston sleeve gear (12), a piston sleeve bearing (13), a piston sleeve bearing sleeve (14), and a piston sleeve bearing lower handle (15). The piston sleeve (11), the piston sleeve gear (12), and the piston sleeve bearing lower handle (15) are fixed together to form a rotating assembly that rotates synchronously. The piston sleeve (11) penetrates the rotating assembly in the axial direction, and a piston-type sliding fit is formed between the solid piston rod (9) and the piston sleeve (11); The driving mechanism includes a linear driving mechanism and a rotary driving mechanism. Among them, the linear driving mechanism cooperates with the piston rod connecting handle (10) to drive the solid piston rod (9) to reciprocate up and down in the piston sleeve (11); the rotary driving mechanism cooperates with the piston sleeve gear (12) to drive the piston sleeve (11) to rotate around its own axis.

2. The piston-type sampling and planting device according to claim 1, wherein, The linear driving mechanism includes a piston rod motor (17), a lead screw (18), a lead screw connecting block (19), a piston rod connecting block (20), a slide rail (21), a slider (22), and a piston rod connecting block buffer spring (23). The piston rod motor (17) and the slide rail (21) are fixed on the bracket (16). The lead screw (18) is connected to the output end of the piston rod motor (17). The lead screw connecting block (19) forms a threaded fit with the lead screw (18). The lead screw connecting block (19) is connected to the bottom of the piston rod connecting block (20). The piston rod connecting block buffer spring (23) is arranged between the bottom of the piston rod motor (17) and the piston rod connecting block (20). The piston rod connecting handle (10) is fixed on the piston rod connecting block (20). The back of the piston rod connecting block (20) is fixedly connected to the slider (22), and the slider (22) forms a sliding fit with the slide rail (21).

3. The piston sampling and planting device according to claim 2, wherein, The piston rod connecting block (20) is provided with a piston rod connecting handle slot (31) for accommodating the piston rod connecting handle (10), and the piston rod connecting handle (10) is embedded and fixed in the piston rod connecting handle slot (31).

4. The piston-type sampling and planting device according to claim 1, wherein, The rotary driving mechanism includes a piston sleeve motor (24), a piston sleeve motor mounting bracket (25), a crank (26), and a piston sleeve rack (27). The piston sleeve motor (24) is mounted on the bracket (16) through the piston sleeve motor mounting bracket (25). The output end of the piston sleeve motor (24) is connected to the crank (26). One end of the piston sleeve rack (27) is provided with a strip hole (28) that cooperates with a crank pin (30) on the crank (26), and the other end of the piston sleeve rack (27) meshes with the piston sleeve gear (12).

5. The piston sampling and planting device according to claim 1, wherein, The linear drive mechanism is a synchronous belt mechanism driven by a motor or a lead screw mechanism driven by a handwheel.

6. The piston-type sampling and planting device according to claim 1, wherein, A piston sleeve bearing sleeve slot (32) for accommodating and fixing the piston sleeve bearing sleeve (14) is provided at the bottom of the bracket (16). The piston sleeve bearing sleeve (14) is fixed in the piston sleeve bearing sleeve slot (32), and the piston sleeve gear (12) is arranged in the piston sleeve bearing sleeve slot (32).

7. The piston-type sampling and planting device according to claim 1, wherein, The piston sleeve bearing (13) includes an upper bearing and a lower bearing.

8. The piston sampling and planting device according to claim 1, wherein, An airtight piston gap is formed between the solid piston rod (9) and the piston sleeve (11).

9. The piston-type sampling and planting device according to claim 1, wherein, A ring knife is formed by machining at the lower end of the piston sleeve (11).

10. The piston sampling and planting device according to claim 1, wherein, The lower handle (15) of the piston sleeve bearing consists of a cylindrical handle body and a limiting flange at the lower end of the handle body. The piston sleeve gear (12) is sleeved on the upper end of the handle body, the piston sleeve bearing (13) is sleeved on the lower part of the handle body, and the piston sleeve bearing (13) is fixed in the piston sleeve bearing (13).

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