Embryo transfer device and method

Abstract

Disclosed in the present application is an embryo transfer device, comprising a clamp, a loading rod and a sheath, wherein the clamp is provided with a structure for mounting the loading rod and the sheath, the loading rod and the sheath are each in embedded-fit with the structure, and acceleration is generated between the loading rod and / or the sheath and a component on the structure by means of a relative acting force, so as to make an embryo on the loading rod fall into the sheath. Embryo transfer can be completed without requiring a microscope and a straw, thus facilitating the use of the embryo transfer device in a ranch and remote areas. In the present application, embryo transfer can be completed without requiring a microscope and a straw, and the transferred embryo is almost damage free, thereby reducing the chance of embryo loss during the transfer process to the maximum extent. The present application also has the characteristics of a simple structure, easy implementation, simple operation, low cost, etc.

Description

Embryo transfer device and method Technical Field

[0001] The present application relates to the field of bioengineering technology, and in particular to an embryo transfer device and method. Background Art

[0002] The use of freeze-thaw embryo technology has significant implications for the livestock industry. First, it provides an effective tool for genetic improvement in livestock. Through frozen embryo technology, the genetic characteristics of high-quality livestock can be preserved and passed down, eliminating the need for continuous live breeding. This means producers can select the best parents to enhance the quality of their offspring, thereby improving livestock production efficiency and profitability. Second, frozen embryos facilitate international trade and the dissemination of genetic resources in the livestock industry. Frozen embryos can be safely transferred between countries, promoting international livestock cooperation and the sharing of genetic resources. This helps improve breeding standards in different regions, enhances breed diversity, and reduces the risk of genetic deficiencies. Furthermore, frozen embryos help reduce the risk of animal disease transmission. Traditional live breeding methods can lead to the spread of disease, but the use of frozen embryos can reduce this risk, contributing to a healthier and more sustainable livestock industry.

[0003] Despite the remarkable success of cryopreservation technology, particularly vitrification, in human medicine and its widespread application in reproductive medicine, its application in the livestock industry has been significantly limited. This limitation is primarily due to factors such as cost, expertise, and pasture environment. First, vitrification requires expensive equipment and expertise, which may be impractical in the livestock industry. Specifically, thawed embryos need to be transferred to recipient cows as quickly as possible. Embryologists use a mouth pipette under a microscope to transfer embryos from the carrier straw to straws, a process that requires meticulous training and a specialized laboratory environment. In pasture environments, there is often a lack of personnel with these specialized skills, making the application of this technology difficult. Furthermore, pastures are often located in remote areas and lack the necessary infrastructure and equipment, such as microscopes and freezing equipment, making the application of the technology even more difficult. Summary of the Invention

[0004] In summary, the present application aims to address at least one defect or deficiency of the above-mentioned prior art and to provide an embryo transfer device and method.

[0005] The present application provides an embryo transfer device, which includes: a clamp, a carrier rod and a sleeve. The clamp is provided with a structure for mounting the carrier rod and the sleeve. The carrier rod and the sleeve are respectively embedded and matched with the structure. The carrier rod and / or the sleeve and the components on the structure generate acceleration through relative force, causing the embryo on the carrier rod to fall into the sleeve.

[0006] The component is fixedly connected to the structure, or movably connected to the structure. The clamp includes a clamp body having a first flat surface and a second flat surface, the second flat surface being located between the two adjacent first flat surfaces and having a height lower than that of the first flat surface. The structure includes a rod mounting portion for mounting a rod and a sleeve mounting portion for mounting a sleeve. The rod mounting portion and the sleeve mounting portion are respectively disposed on the first flat surface of the clamp. The rod mounting portion is provided with one or more rod mounting slots for mounting rods, and the sleeve mounting portion is provided with one or more sleeve mounting slots for mounting sleeves. The rods and sleeves respectively engage with the rod mounting slots and the sleeve mounting slots. A pair of lugs are provided on opposite side walls of each rod mounting slot and sleeve mounting slot for engaging with the rods and sleeves. Each lug has a gap between it and the inner wall of the rod mounting portion or the sleeve mounting portion. The spacing between the lugs is less than or equal to the spacing between the rod mounting slots or the sleeve mounting slots. When the carrier rod is inserted into the carrier rod mounting slot, the lugs deform and move toward the gap. Friction between the carrier rod and the components generates acceleration, causing the embryos on the carrier rod to fall into the sleeve. The components are selected from the group consisting of a rack, a raised plate, and an embossed plate. A pair of these components are positioned opposite each other on one or both inner walls of each carrier rod mounting slot. The components are integrally formed with the carrier rod mounting portion or are flexibly connected to the carrier rod mounting portion. The integral forming process includes bonding, CNC machining, injection molding, laser engraving, etching, or 3D printing. The flexibly connected connection includes an elastic connection or riveting. The carrier rod comprises a handle, a carrier rod body, and a front plate. The front plate has a first groove that holds the embryos and / or culture medium. A base supports the clamp. The base has a second groove corresponding to the shape of the clamp, and the clamp is positioned in the second groove. An alignment device is used to position the carrier rod for easy insertion into the sleeve. The alignment device is integrally formed with or flexibly connected to the second flat surface. The alignment device includes a plug-in portion and a positioning portion, wherein the plug-in portion is fixedly connected to the positioning portion. At least one carrier rod positioning hole and at least one sleeve positioning hole are respectively provided at both ends of the positioning portion, and the carrier rod positioning hole and the sleeve positioning hole are connected. The carrier rod positioning hole and the sleeve positioning hole are trumpet-shaped and gradually expand from the inside to the outside. An opening is provided above the carrier rod positioning hole to facilitate the user to observe the insertion status. The embryo falls off from the first groove of the thin plate at the front end of the carrier rod into the liquid or on a solid surface. The carrier rod is placed in the sleeve, or the carrier rod is plugged into the sleeve. There is a gap between the carrier rod and the sleeve for liquid and / or embryos to pass through.

[0007] The cannula is selected from the group consisting of a straw, a centrifuge tube, and a test tube. The relative force is friction and / or elastic force generated by deformation. The acceleration is used to induce movement of the structure containing the embryo. The movement is generated in a reciprocating manner. The acceleration is multiple accelerations. The number of accelerations is 1-500. The negative pressure device is used to draw liquid from the test tube into the cannula. The negative pressure device can be a manual negative pressure device or an automatic negative pressure device. The manual negative pressure device can be selected from the group consisting of a syringe, a rubber-tipped pipette, or an oral pipette. The automatic negative pressure device can be selected from the group consisting of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump. The negative pressure device draws culture fluid from the test tube and injects it into the cannula, forming at least one section of liquid within the cannula. The liquid can be selected from the group consisting of culture fluid, transplantation fluid, or operating fluid. The automatic negative pressure device comprises a housing, a circuit board, and a motor. The circuit board and motor are mounted within the housing. Control buttons and a display screen are provided on the surface of the housing. The motor, control buttons, and display screen are electrically connected to the circuit board. The automatic negative pressure device has a liquid inlet and a liquid outlet. The liquid inlet is connected to the external test tube through a first connecting tube, and the liquid outlet is connected to the sleeve through a second connecting tube. The automatic negative pressure device absorbs the liquid in the test tube through negative pressure and injects the liquid into the sleeve.

[0008] The present application also provides an embryo transfer method, which comprises the following steps:

[0009] a. Aspirate the liquid in the test tube to form at least one section of liquid in the cannula;

[0010] b. Insert the front end sheet of the carrier rod into the sleeve, so that the carrier rod and the sleeve are respectively engaged with the clamp;

[0011] c. Remove the sleeve from the fixture;

[0012] d. Aspirate the liquid in the test tube again to form at least one section of liquid in the sleeve.

[0013] The step a is preceded by the following step: inserting the cannula into the rubber stopper, and the test tube contains liquid.

[0014] The method of aspirating the liquid in the test tube in step a can be manual or automatic.

[0015] The step a is followed by the following steps: placing the syringe and the cannula on a fixture and adjusting the relative position of the liquid in the cannula.

[0016] In step b, the carrier rod and / or sleeve and the clamp generate acceleration through relative force, so that the embryo on the carrier rod falls into the sleeve.

[0017] After step d, the following step is further included: removing the sleeve from the rubber plug and placing it into a transplant gun for embryo transplantation.

[0018] The relative acting force is friction force and / or elastic force generated by deformation.

[0019] The liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

[0020] By adopting the above technical solution, compared with the existing technology, the beneficial effect produced by the present application is that: the present application provides an elastic lug on the clamp, and a rack arranged on the lug, and generates acceleration through the friction between the rack and the carrier rod, so that the embryo on the carrier rod is transferred to the sleeve. The embryo transfer can be completed without the need for a microscope and a mouth pipette, which is convenient for use in pastures and remote environments.

[0021] The embryos transferred by this application are almost undamaged, and the loss of embryos during the transfer process is avoided to the greatest extent. This application also has the characteristics of simple structure, easy implementation, simple operation (only requires simple learning to use, and no embryologist is required to be present), and low cost. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] FIG1 is a schematic diagram of the overall structure of Example 1 of the present application.

[0023] FIG2 is a schematic structural diagram of the clamp of the present application.

[0024] FIG3 is a top view of the clamp of the present application.

[0025] FIG4 is a partially enlarged schematic diagram of part I in FIG3 of the present application.

[0026] FIG5 is a partially enlarged schematic diagram of part II in FIG3 of the present application.

[0027] FIG6 is a schematic structural diagram of the base of the present application.

[0028] FIG7 is a schematic structural diagram of the alignment device of the present application.

[0029] FIG8 is another schematic structural diagram of the alignment device of the present application.

[0030] FIG9 is a schematic structural diagram of the carrier rod of the present application.

[0031] Figure 10 is a schematic diagram of the overall structure of Example 2 of the present application.

[0032] FIG11 is a schematic diagram of the overall structure of Example 2 of the present application.

[0033] Figure 12 is a schematic structural diagram of the clamp of Example 2 of the present application.

[0034] Figure 13 is a schematic diagram of the connection between the automatic negative pressure device and the sleeve in Example 1 of the present application.

[0035] Figure 14 is a schematic diagram of the decomposition of the automatic negative pressure device of Example 1 of the present application.

[0036] Figure 15 is a three-dimensional schematic diagram of the automatic negative pressure device of Example 1 of the present application. DETAILED DESCRIPTION

[0037] The following examples are provided to further explain and supplement the present application and do not constitute any limitation to the present application.

[0038] The technical solution of the present application is further described below with reference to the accompanying drawings and embodiments.

[0039] Example 1:

[0040] As shown in Figures 1 to 12, an embryo transfer device of the present application includes: a clamp 10, a carrier rod 20 and a sleeve 30. The clamp 10 is provided with a structure 40 for mounting the carrier rod and the sleeve. The carrier rod 20 and the sleeve 30 are respectively embedded in and matched with the structure. The carrier rod 20 and / or the sleeve 30 and the component 43 on the structure 40 generate acceleration through relative force, causing the embryo on the carrier rod 20 to fall off into the sleeve 30.

[0041] Furthermore, the component 43 is fixedly connected to the structure 40 , or the component 43 is movably connected to the structure 40 .

[0042] In this embodiment, the component 43 and the structure 40 are fixedly connected.

[0043] As shown in FIG2 , the clamp further includes a clamp body 13 , the clamp body 13 having a first plane 14 and a second plane 15 , the second plane 15 is located between the two adjacent first planes 14 , and the height of the second plane 15 is lower than that of the first plane 14 .

[0044] Furthermore, the structure 40 includes a rod mounting portion 41 for mounting a rod and a sleeve mounting portion 42 for mounting a sleeve.

[0045] Furthermore, the rod mounting portion 41 and the sleeve mounting portion 42 are respectively arranged on the first plane 14 of the clamp.

[0046] As shown in Figures 4 and 5 , the rod mounting portion 41 further comprises one or more rod mounting grooves 411 for mounting rods, and the rods 20 fit into the rod mounting grooves 411. The sleeve mounting portion 42 comprises one or more sleeve mounting grooves 421 for mounting sleeves, and the sleeves 30 fit into the sleeve mounting grooves 421.

[0047] As shown in Figures 4 and 5, further, a pair of lugs 412 and 422 are respectively provided on both side walls of each of the carrier rod installation groove 411 and the sleeve installation groove 421, which are engaged with the carrier rod 20 and the sleeve 30. In this embodiment, the lugs are elastic. When the carrier rod or the sleeve is placed, the lugs are squeezed and deformed. When the carrier rod or the sleeve is taken out, the lugs return to their original shape.

[0048] As shown in FIG. 4 and FIG. 5 , further, a gap 413 , 423 is respectively defined between each of the lugs 412 , 422 and the inner wall of the rod mounting portion 41 or the sleeve mounting portion 42 .

[0049] Furthermore, the distance between the pair of lugs 412 and 422 is less than or equal to the distance between the rod mounting groove 411 or the sleeve mounting groove 421 .

[0050] In this embodiment, the distance between the pair of lugs 412 and 422 is smaller than the distance between the rod mounting groove 411 or the sleeve mounting groove 421 .

[0051] Furthermore, the carrier rod 20 is inserted into the carrier rod installation groove 411 , the lugs 412 and 422 are deformed and move toward each other toward the gap, and the carrier rod 20 and the components generate acceleration through friction, causing the embryo on the carrier rod 20 to fall into the sleeve 30 .

[0052] In this embodiment, the component 43 is one of a rack, a raised plate, and an embossed plate.

[0053] Preferably, the component is a rack.

[0054] As shown in FIG. 4 , further, a pair of the components 43 are respectively and oppositely arranged on one side inner wall or both sides inner walls of each of the carrying rod installation slots 411 .

[0055] Furthermore, the component 43 is integrally formed with the rod mounting portion 41 , or the component 43 is movably disposed on the rod mounting portion 41 .

[0056] Furthermore, the one-piece molding processing method includes one of bonding, CNC machine tool processing, injection molding, laser engraving, etching, and 3D printing, and the movable connection includes one of elastic connection and riveting.

[0057] As shown in FIG9 , specifically, the carrier rod 20 includes a handle 21 , a carrier rod body 22 and a front sheet 23 . The front sheet has a first groove 231 . The first groove 231 contains embryos and / or culture medium, and of course also includes but is not limited to biological materials such as eggs and cells, which is not limited in this application.

[0058] As shown in Figure 6, the present application also includes a base 50 for supporting the clamp, and the base 50 is provided with a second groove 51 corresponding to the shape of the clamp 10. The clamp 10 is set in the second groove 51. The weight of the base is greater than the weight of the clamp, so when the clamp is placed in the base, the clamp can be made more stable and less likely to shake.

[0059] As shown in FIG. 2 and FIG. 3 , further, a third groove 151 extending in the width direction is provided on the second plane 15 .

[0060] As shown in FIG. 7 and FIG. 8 , the present application further includes an alignment device 60 for positioning and guiding the carrier rod so that it can be easily plugged into the sleeve. The alignment device 60 is integrally formed or plugged into the second plane 15 .

[0061] Furthermore, the alignment device 60 is plugged into the third groove 151 .

[0062] As shown in Figures 7 and 8, specifically, the alignment device 60 includes an inserting portion 61 and a positioning portion 62. The inserting portion 61 is fixedly connected to the positioning portion 62. At least one rod positioning hole 621 and at least one sleeve positioning hole 622 are respectively opened at both ends of the positioning portion. The rod positioning hole 621 is connected to the sleeve positioning hole 622.

[0063] As shown in FIG. 7 , the rod positioning hole 621 and the sleeve positioning hole 622 are trumpet-shaped and gradually expand from the inside to the outside. An opening 6211 is provided above the rod positioning hole 621 for the user to conveniently observe the insertion status.

[0064] Furthermore, the embryo falls off from the first groove 231 of the front end sheet of the carrier rod into the liquid or solid surface.

[0065] Furthermore, the carrying rod 20 is placed in the sleeve 30, or the carrying rod 20 is plugged into the sleeve 30. In this embodiment, the carrying rod 20 is plugged into the sleeve 30.

[0066] Furthermore, there is a gap between the carrier rod 20 and the sleeve 30 for liquid and / or embryos to pass through.

[0067] Furthermore, the sleeve 30 is one of a wheat straw, a centrifuge tube, and a test tube.

[0068] In this embodiment, preferably, the sleeve is a wheat straw.

[0069] Furthermore, the relative force is friction force and / or elastic force generated by deformation.

[0070] Furthermore, the acceleration is used to move a structure containing the embryo.

[0071] Furthermore, the motion is generated in a reciprocating manner.

[0072] Furthermore, the acceleration is multiple accelerations.

[0073] Furthermore, the number of accelerations is 1-500 times.

[0074] As shown in Figures 13 to 15, the present application also includes a negative pressure device for sucking the liquid in the test tube into the sleeve.

[0075] Furthermore, the negative pressure device is a manual negative pressure device (not shown in the figure) or an automatic negative pressure device 50.

[0076] The manual negative pressure device is one of a syringe, a rubber-tipped straw or an oral straw, and the automatic negative pressure device is one of a syringe pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump.

[0077] Furthermore, the negative pressure device absorbs the liquid in the test tube and injects it into the sleeve, so that at least one section of liquid is formed in the sleeve.

[0078] Specifically, the liquid is one of a culture fluid, a transplantation fluid or an operating fluid.

[0079] As shown in Figures 13 and 14, the automatic negative pressure device 50 specifically includes a housing 51, within which is mounted a circuit board 52 and a motor 53. A control button 54 and a display screen 55 are provided on the surface of the housing 51. The motor 53, control button 54, and display screen 55 are electrically connected to the circuit board. In this embodiment, the control button 54 is used to turn the automatic negative pressure device on and off, and the display screen 55 is used to display specific values ​​for the suction and injection of liquid into the cannula.

[0080] As shown in Figure 13, further, the automatic negative pressure device 50 has a liquid inlet and a liquid outlet (not shown in the figure), the liquid inlet is connected to an external test tube (not shown in the figure) through a connecting tube (not shown in the figure), and the liquid outlet is connected to the sleeve 30 through a connecting tube 56. The automatic negative pressure device absorbs the liquid in the test tube through negative pressure and injects the liquid into the sleeve.

[0081] The present application also includes an embryo transfer method, which uses a syringe to draw liquid from a test tube and inject the liquid into a cannula. The method specifically includes the following steps:

[0082] a. First, remove the syringe with the rubber stopper, cannula and test tube containing the culture medium;

[0083] b. Insert the cannula into the rubber stopper (it is better to cover the first section of cotton wool), and then use a syringe to draw culture medium from the test tube to form three sections of liquid in the cannula;

[0084] c. Place the syringe and cannula in the fixture and adjust the position of the third liquid;

[0085] d. Remove the automatically thawed embryos, substrate, straw support rack, and straws from the external machine, and remove the straws from the straw support rack.

[0086] e. Insert the front end sheet of the carrier rod into the sleeve, and clamp the carrier rod and sleeve together into the clamp lug;

[0087] f. Remove the carrier rod from the fixture from the lug;

[0088] g. Remove the syringe and cannula from the fixture, and use the syringe to draw culture fluid from the test tube to form five sections of liquid in the cannula, with the first section of liquid in contact with the second section of cotton wool;

[0089] Remove the cannula from the rubber stopper and place it in the transplant gun for embryo transplantation.

[0090] The present application also includes an embryo transfer method, which uses an automatic negative pressure device to suck liquid from a test tube through negative pressure and inject the liquid into a cannula. The method includes the following steps:

[0091] a. Remove the automatic negative pressure device with 1-10 rubber stoppers, the cannula, and the test tube containing the culture medium;

[0092] Insert the cannula into the rubber stopper (it is best to cover the first section of cotton wool), turn on the automatic negative pressure device to absorb the culture medium and air in the test tube to form three sections of liquid in the cannula;

[0093] b. Place the sleeve on the fixture;

[0094] c. Remove the automatically thawed embryos, substrate, straw support rack, and straws from the external machine, and remove the straws from the straw support rack;

[0095] d. Insert the front end sheet of the carrier rod into the sleeve, and clamp the carrier rod and sleeve together into the clamp lug;

[0096] Remove the carrier rod from the clamp from the lug;

[0097] e. Remove the cannula from the fixture and use the automatic negative pressure device to draw culture fluid into the test tube to form five sections of liquid in the cannula, with the first section of liquid in contact with the second section of cotton wool;

[0098] f. Remove the cannula from the rubber stopper and place it into the transplant gun for embryo transplantation.

[0099] Example 2

[0100] Figures 10 to 12 are schematic structural diagrams of another embodiment of the present application. The basic structure and operating steps of this embodiment are the same as those of Example 1. The components with the same reference numerals in Figures 10 to 12 have the same functions as those in Figure 1. For the sake of simplicity, the description of these same components is omitted. The differences are as follows:

[0101] In this embodiment, as shown in FIG11 , a plurality of components 43 are disposed on the second plane 15 of the fixture, and each component 43 is perpendicular to the second plane 15 .

[0102] Furthermore, every two components form a group and are arranged opposite to each other, and multiple groups of components are horizontally arranged along the length direction of the second plane.

[0103] Furthermore, the teeth on each group of relatively arranged components can be arranged with both sides facing inward, or both sides facing outward, or the teeth on one group of components can be arranged with both sides facing inward, and the teeth on the adjacent group of components can be arranged with both sides facing outward. The purpose of such arrangement is to ensure that the carrier rod placed in the carrier rod mounting groove is in full contact with each component to generate friction.

[0104] As shown in FIG12 , in this embodiment, a plurality of lugs 412 , 422 are respectively provided on both side walls of the first plane 14 and the second plane 15 , and the plurality of lugs 412 , 422 are arranged opposite to the components.

[0105] As shown in Figure 12, in this embodiment, the alignment device 60 is disposed on the first plane 14 and located between the rod mounting portion and the sleeve mounting portion. The alignment device 60 includes a base 61 and a guide groove 62. The front end sheet 23 of the rod is disposed in the guide groove 62, and the front end sheet portion of the rod is positioned in the guide groove 62.

[0106] Although the present application is disclosed through the above embodiments, the scope of the present application is not limited thereto. Without departing from the concept of the present application, the above components can be replaced with similar or equivalent elements known to those skilled in the art.

Claims

1. An embryo transfer device, characterized in that, Including: A fixture (10), a carrier rod (20) and a sleeve (30). A structure (40) for mounting the carrier rod and the sleeve is provided on the fixture (10). The carrier rod (20) and the sleeve (30) are respectively fitted into the structure in an embedded manner. The carrier rod (20) and / or the sleeve (30) and a component (43) on the structure (40) generate an acceleration through a relative acting force, so that the embryo on the carrier rod (20) falls off into the sleeve (30).

2. The embryo transfer device according to claim 1, wherein, The component (43) is fixedly connected to the structure (40), or the component (43) is movably connected to the structure (40).

3. The embryo transfer device according to claim 1, characterized in that, The fixture includes a fixture body (13). A first plane (14) and a second plane (15) are provided on the fixture body (13). The second plane (15) is located between two adjacent first planes (14), and the height of the second plane (15) is lower than the height of the first plane (14).

4. The embryo transfer device according to claim 1, characterized in that, The structure (40) includes a carrier rod mounting portion (41) for mounting the carrier rod and a sleeve mounting portion (42) for mounting the sleeve.

5. The embryo transfer device according to claim 4, wherein The carrier rod mounting portion (41) and the sleeve mounting portion (42) are respectively provided on the first plane (14) of the fixture.

6. The embryo transfer device according to claim 5, characterized in that, One or more carrier rod mounting grooves (411) for mounting the carrier rod are provided on the carrier rod mounting portion (41). One or more sleeve mounting grooves (421) for mounting the sleeve are provided on the sleeve mounting portion (42). The carrier rod (20) and the sleeve (30) are respectively fitted into the carrier rod mounting groove (411) and the sleeve mounting groove (421) in an embedded manner.

7. The embryo transfer device according to claim 6, wherein, On both side walls of each carrier rod mounting groove (411) and sleeve mounting groove (421), a pair of lugs (412, 422) for cooperating with and clamping the carrier rod (20) and the sleeve (30) are respectively provided opposite to each other.

8. The embryo transfer device according to claim 7, characterized in that, A gap (413, 423) is respectively provided between each lug (412, 422) and the inner wall of the carrier rod mounting portion (41) or the sleeve mounting portion (42).

9. The embryo transfer device according to claim 8, wherein The distance between the pair of lugs (412, 422) is less than or equal to the distance between the carrier rod mounting groove (411) or the sleeve mounting groove (421).

10. The embryo transfer device according to claim 9, wherein, The carrier rod (20) is inserted into the carrier rod mounting groove (411), the lugs (412, 422) are deformed and move towards each other to the gap. The carrier rod (20) and the component (43) generate an acceleration through friction, so that the embryo on the carrier rod (20) falls off into the sleeve (30).

11. The embryo transfer device according to claim 10, wherein, The component (43) is one of a rack, a raised plate and an embossed plate.

12. The embryo transfer device according to claim 11, wherein A pair of the components (43) are respectively provided opposite to each other on one side inner wall or both side inner walls of each carrier rod mounting groove (411).

13. The embryo transfer device according to claim 12, characterized in that, The component (43) is integrally formed with the carrier rod mounting portion (41), or the component (43) is movably provided on the carrier rod mounting portion (41).

14. The embryo transfer device according to claim 13, characterized in that, The integrally formed processing method includes one of bonding, numerical control machine tool processing, injection molding, laser engraving, etching, 3D printing. The movable connection includes one of elastic connection and riveting.

15. The embryo transfer device according to claim 10, wherein, The carrier rod (20) includes a handle (21), a carrier rod body (22) and a front-end thin sheet (23). A first groove (231) is formed on the front-end thin sheet, and embryos and / or culture solution are contained in the first groove (231).

16. The embryo transfer device according to claim 1, characterized in that, It further includes a base (50) for supporting the fixture. A second groove (51) corresponding to the shape of the fixture is provided on the base (50), and the fixture (10) is arranged in the second groove (51).

17. The embryo transfer device according to claim 3, characterized in that, It further includes an alignment device (60) for positioning the carrier rod to facilitate its insertion into the sleeve. The alignment device (60) is integrally formed or inserted with the second plane (15).

18. The embryo transfer device according to claim 17, wherein The alignment device (60) includes a plug-in portion (61) and a positioning portion (62). The plug-in portion (61) is fixedly connected to the positioning portion (62). At least one carrier rod positioning hole (621) and at least one sleeve positioning hole (622) are respectively formed at both ends of the positioning portion. The carrier rod positioning hole (621) communicates with the sleeve positioning hole (622).

19. The embryo transfer device according to claim 18, characterized in that, The carrier rod positioning hole (621) and the sleeve positioning hole (622) are flared and gradually expand from inside to outside. An opening (6211) for facilitating the user to observe the insertion state is formed above the carrier rod positioning hole (621).

20. The embryo transfer device according to claim 15, characterized in that, The embryos fall from the first groove (231) at the front-end thin sheet of the carrier rod into the liquid or onto the solid surface.

21. The embryo transfer device according to claim 20, characterized in that, The carrier rod (20) is placed in the sleeve (30), or the carrier rod (20) is inserted into the sleeve (30).

22. The embryo transfer device according to claim 21, wherein, A gap for allowing liquid and / or embryos to pass through is provided between the carrier rod (20) and the sleeve (30).

23. The embryo transfer device according to claim 22, characterized in that, The sleeve (30) is one of a straw, a centrifuge tube, and a test tube.

24. The embryo transfer device according to claim 1, characterized in that, The relative acting force is the frictional force and / or the elastic force generated by deformation.

25. The embryo transfer device according to claim 10, characterized in that, The acceleration is used to cause the structure containing the embryos to move.

26. The embryo transfer device according to claim 25, characterized in that, The way of generating the movement is reciprocating movement.

27. The embryo transfer device according to claim 25, wherein, The acceleration is multiple accelerations.

28. The embryo transfer device according to claim 27, wherein, The number of times of the acceleration is 1 - 500 times.

29. The embryo transfer device according to claim 1, characterized in that, It further includes a negative pressure device for sucking the liquid in the test tube into the sleeve.

30. The embryo transfer device according to claim 29, wherein, The negative pressure device is a manual negative pressure device or an automatic negative pressure device (50).

31. The embryo transfer device according to claim 30, wherein, The manual negative pressure device is one of a syringe, a rubber-tipped pipette, and a mouth pipette. The automatic negative pressure device is one of an injection pump, a peristaltic pump, a plunger pump, a diaphragm pump, a vacuum pump, a centrifugal pump, an impeller pump, and a piston pump.

32. The embryo transfer device according to claim 30, wherein, The negative pressure device sucks the liquid in the test tube and injects it into the sleeve, so that at least one section of liquid is formed in the sleeve.

33. The embryo transfer device according to claim 32, wherein, The liquid is one of a culture solution, a transplantation solution, and an operation solution.

34. The embryo transfer device according to claim 31, wherein, The automatic negative pressure device (50) includes a housing (51), a circuit board (52), and a motor (53). The circuit board (52) and the motor (53) are installed in the housing (51). A control button (54) and a display screen (55) are provided on the surface of the housing (51). The motor (53), the control button (54), and the display screen (55) are respectively electrically connected to the circuit board (52).

35. The embryo transfer device according to claim 31, wherein, The automatic negative pressure device (50) is provided with a liquid inlet and a liquid outlet. The liquid inlet is connected to an external test tube through a first connecting pipe, and the liquid outlet is connected to the sleeve (30) through a second connecting pipe (56). The automatic negative pressure device sucks the liquid in the test tube and injects the liquid into the sleeve.

36. An embryo transfer method, characterized in that, The method includes the following steps: a. Suck the liquid in the test tube to form at least one section of liquid in the sleeve; b. Insert the front thin sheet of the carrier rod into the sleeve, and make the carrier rod and the sleeve respectively cooperate with the clamp for clamping; c. Remove the sleeve on the clamp; d. Suck the liquid in the test tube again to form at least one section of liquid in the sleeve.

37. The embryo transfer method according to claim 36, characterized in that, Before step a, there is also the following step: Insert the sleeve into the rubber stopper, and there is liquid in the test tube.

38. The embryo transfer method according to claim 36, wherein The way of sucking the liquid in the test tube in step a can be manual or automatic.

39. The embryo transfer method according to claim 36, wherein After step a, there is also the following step: Place the syringe and the sleeve on the clamp and adjust the relative position of the liquid in the sleeve.

40. The embryo transfer method according to claim 36, wherein, In step b, the carrier rod and / or the sleeve and the clamp generate acceleration through relative acting forces, so that the embryo on the carrier rod falls off into the sleeve.

41. The embryo transfer method according to claim 36, characterized in that, After step d, there is also the following step: Take out the sleeve from the rubber stopper, and install it into the transplantation gun for embryo transplantation.

42. The embryo transfer method according to claim 40, characterized in that, The relative acting force is the frictional force and / or the elastic force generated by deformation.

43. The embryo transfer method according to claim 36, wherein The liquid is one of culture medium, transplantation liquid or operation liquid.

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