Hazard factor test cassette and method

Abstract

A hazard factor test cassette and method. The hazard factor test cassette comprises: flow guide paths, the plurality of flow guide paths being respectively and independently communicated with a sample injection port (1) and flow guide platforms (2); each flow guide path comprises a long-distance flow guide path (3) and a short-distance flow guide path (4); the length for the long-distance flow guide paths (3) connecting the sample injection port (1) and the flow guide platforms (2) is greater than that of the short-distance flow guide paths (4); test strips for testing small-size targets are provided on the flow guide platforms (2) corresponding to the long-distance flow guide paths (3); and test strips for testing large-size targets are provided on the flow guide platforms (2) corresponding to the short-distance flow guide paths (4). The hazard factor test method uses the hazard factor test cassette for test, and comprises sequentially arranging toxin type test strips, virus type test strips and bacterium type test strips on paths from the long-distance flow guide paths (3) to the short-distance flow guide paths (4) among the short-distance flow guide paths (4) and the long-distance flow guide paths (3).

Description

Hazard factor detection reagent card and detection method

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application claims priority to a Chinese patent application filed with the Patent Office of China on March 1, 2024, with application number 202410235002.2 and invention name “A Hazard Factor Detection Reagent Card and Detection Method,” the entire contents of which are incorporated by reference into this application. Technical Field

[0003] The present application relates to the field of immunodiagnosis technology, and in particular to a hazard factor detection reagent card and detection method based on biological probe multi-channel immunochromatography. Background Art

[0004] The hazard factor detection reagent card adopts immunochromatography technology. Immunochromatography technology is a relatively mature on-site rapid detection technology. Multi-channel immunochromatography test paper discs are disclosed in the prior art. The existing multi-channel immunochromatography test paper discs and the prior patents of the present patent applicant, such as the patent application with publication number CN101261270A, disclose that: the unique multi-stage design and drainage pad of the test paper disc are used to achieve uniform distribution of the sample solution between the test papers in each channel and the subsequent synchronous chromatography of the sample solution, so as to achieve simultaneous detection of multiple targets. However, there are still some problems. Since there is no strict flow channel distinction from the addition of the sample to the chromatography of the multiple target detection reagent strips, there is a situation where the sample solution refluxes after contacting the sample pad and the labeling pad of the reagent strip and the adjacent channels are disturbed, which affects the sensitivity and specificity of the detection; the current structure can only achieve the synchronous chromatography of the sample solution on the multi-channel detection reagent strip, but not the synchronous chromatography of the target to be detected.

[0005] Due to the wide variety of biohazard factors and the different sizes of different types of biohazard factors, the biohazard factor detection reagent cards in the existing technology lack the design to prevent flow disturbance and the synchronous design of chromatographic detection of multiple types of targets for bacteria, viruses, toxins, etc. For example, bacteria, viruses, and toxins are particles dispersed and suspended in a solvent, and there are differences in size and resistance. When bacteria, viruses, and toxins pass through the drainage pad, sample pad, labeling pad, analytical membrane and other reagent strips with micro-pore structures along with the universal sample processing fluid, the larger bacterial particles experience the greatest resistance and the slowest chromatography, while the smallest toxins experience the least resistance and the fastest chromatography. In order to achieve synchronous chromatography of biohazard factor targets of different sizes on the same detection reagent card and improve detection sensitivity, different target detection reagent strips are allocated to different drainage stations and drainage channels of different lengths according to different target sizes.

[0006] Summary of the Invention

[0007] Therefore, the technical problem to be solved by this application is how to address the lack of biohazard factor detection technology in the existing technology that can achieve simultaneous chromatography of multiple targets at once for bacteria, viruses, toxins, etc. To this end, this application provides a hazard factor detection reagent card, which is a hazard factor detection reagent card based on multi-channel immunochromatography using biological probes, comprising:

[0008] outer shell;

[0009] Sample port, used for adding samples;

[0010] Drainage tables, the number of which ranges from 2 to 20, and the drainage tables are used to accommodate individual detection reagent strips;

[0011] Drainage channels, the number of which is 2 to 20, each of which independently connects the sample loading port and the drainage table; the drainage channels include: a long-distance drainage channel and a short-distance drainage channel; the length of the long-distance drainage channel connecting the sample loading port and the drainage table is greater than that of the short-distance drainage channel; a detection reagent strip for detecting small-sized targets is arranged on the drainage table corresponding to the long-distance drainage channel, and a detection reagent strip for detecting large-sized targets is arranged on the drainage table corresponding to the short-distance drainage channel.

[0012] Optionally, the outer shell includes: an upper shell and a lower shell that are assembled and connected;

[0013] The drainage channel includes: a drainage groove provided on one of the upper shell and the lower shell, and a drainage plate provided on the other of the upper shell and the lower shell;

[0014] When the outer shell is in an assembled state, the drainage piece is inserted and fixed in the drainage groove.

[0015] Optionally, the drainage piece and the drainage groove are connected by interference fit.

[0016] Optionally, the hazard factor detection reagent card further includes: a rivet structure provided on one of the upper shell and the lower shell, and a ring groove structure provided on the other of the upper shell and the lower shell;

[0017] The rivet structure and the annular groove structure are plugged together to fix the upper shell and the lower shell together.

[0018] Optionally, the rivet structure and the annular groove structure that are connected in cooperation are arranged on a side of the outer shell away from the drainage channel.

[0019] Optionally, a plurality of drainage channels are arranged in the width direction of the outer shell;

[0020] The sample addition port is an elliptical structure extending along the width direction of the outer shell. The long-distance drainage channel and / or the short-distance drainage channel has: a smooth curved section extending toward the sample addition port, and a connecting section extending toward the drainage platform.

[0021] Optional hazard factor detection reagent card also includes:

[0022] A wide-view detection window is used to observe the chromatography status of the detection reagent strip and the final detection result.

[0023] Optionally, there is one wide-view detection window on the outer shell, and the wide-view detection window is correspondingly arranged on the central axis of the sample injection port; the long-distance drainage channel and the short-distance drainage channel are both arranged opposite to the sample injection port; or,

[0024] There are two wide-view detection windows on the outer shell, and the two wide-view detection windows are symmetrically arranged on both sides of the sample addition port; the long-distance drainage channel and the short-distance drainage channel are arranged on both sides of the sample addition port.

[0025] Optionally, a baffle for separating the detection reagent strips is provided on the lower shell, and a fixing column for positioning the detection reagent strips is further provided on the upper shell.

[0026] A method for detecting biohazardous factors, using the above-mentioned biohazardous factor detection reagent card, comprising:

[0027] Step S1: Preparation of potent biohazard agent detection reagent strips and preparation of universal sample processing solution;

[0028] Step S2: Place the test strips for identifying different types of potent biohazardous factors on different drainage platforms of the multi-channel test reagent card, place drainage pads at the sample loading position, and cover and tighten the upper cover;

[0029] Step S3: Add 1 mL to 3 mL of the sample prepared with the universal sample treatment solution to the sample injection port, and record the chromatography time to determine the detection time;

[0030] Step S4: inserting the reagent strip end of the reagent card into the detector to a fixed position;

[0031] Step S5: Start the detector to perform quantitative measurement on the test strip through the wide-view detection window, and the instrument displays the measurement results;

[0032] Step S6: All negative samples are added to the sample injection port of the test reagent card, and the results are read on the machine. Each test reagent strip has a clear quality control peak and a very low test peak. The ratio of the test peak to the quality control peak shows a negative result;

[0033] Step S7: The sample to be tested prepared with the universal sample treatment solution is added to the sample well of the multi-channel detection reagent card, and the results are read on the instrument. The ratio of the test peak value to the quality control peak value is regressed with the instrument's built-in standard curve to determine the result of a certain potent biohazard factor in the sample;

[0034] Wherein, in step S2:

[0035] placing a detection reagent strip for detecting large-sized targets on the short-distance drainage channel, and placing a detection reagent strip for detecting small-sized targets on the long-distance drainage channel;

[0036] Among them, the bacteria detection reagent strip, the virus detection reagent strip and the toxin detection reagent strip are sequentially arranged on the channels from short distance to long distance in the short distance drainage channel and the long distance drainage channel.

[0037] The technical solution of this application has the following advantages:

[0038] 1. The hazard factor detection reagent card provided in this application comprises: an outer shell; a sample injection port for injecting a sample; and a drainage platform, wherein the number of the drainage platforms ranges from 2 to 20, and the drainage platforms are used to accommodate individual detection reagent strips;

[0039] Drainage channels, the number of which is 2 to 20, each of which independently connects the sample loading port and the drainage table; the drainage channels include: a long-distance drainage channel and a short-distance drainage channel; the length of the long-distance drainage channel connecting the sample loading port and the drainage table is greater than that of the short-distance drainage channel; a detection reagent strip for detecting small-sized targets is arranged on the drainage table corresponding to the long-distance drainage channel, and a detection reagent strip for detecting large-sized targets is arranged on the drainage table corresponding to the short-distance drainage channel.

[0040] In the present application, the drainage channel is divided into a long-distance drainage channel and a short-distance drainage channel. At the same time, a detection reagent strip for detecting small-sized targets is arranged on the long-distance drainage channel, and a detection reagent strip for detecting large-sized targets is arranged on the short-distance drainage channel. The multi-channel detection reagent card can be made compatible with the detection of three types of targets: bacteria, viruses, and toxins. According to the size and resistance characteristics of bacteria, viruses, and toxins, the drainage fixed structure and the reagent strip distribution method are relied on to synchronize the progress of chromatography of different types of targets to be detected, such as bacteria, viruses, and toxins, on their respective detection reagent strips, so as to achieve consistency in target chromatography and capture time of multi-channel, so as to ensure that the detection result sensitivity of multi-channel is the highest.

[0041] For bacteria and toxins dispersed in the sample treatment fluid, the resistance during the chromatography process is different due to the different particle sizes. The chromatography process exists in the drainage pad and each part of the detection reagent strip that the sample flows through after being added to the sample injection port. The target flows through the above-mentioned material with a porous structure with the sample treatment fluid and performs important reactions such as specific identification and specific capture. The chromatography and reaction time of the target in the sample are directly related to the detection sensitivity. Bacteria with larger sizes are subject to greater resistance during the chromatography process, and the chromatography of bacterial targets is slower. Toxins with smaller sizes are subject to less resistance during the chromatography process, and the chromatography of toxin targets is faster. Therefore, in this application, the bacterial detection reagent strip is set in the short-distance drainage channel, and the toxin detection reagent strip is set in the long-distance drainage channel.

[0042] 2. The hazard factor detection reagent card provided in the present application, wherein the outer shell includes: an upper shell and a lower shell that are assembled and connected; the drainage channel includes: a drainage groove respectively arranged on one of the upper shell and the lower shell, and a drainage piece arranged on the other of the upper shell and the lower shell; when the outer shell is in the assembled state, the drainage piece is inserted and fixed in the drainage groove.

[0043] In the present application, a drainage channel is formed by assembling and plugging the connected drainage grooves and drainage sheets. The above-mentioned drainage grooves and drainage sheets connected by the plug-in assembly effectively block the sample processing liquid from flowing out of the gaps between the different drainage channels between the upper and lower shells. This not only effectively blocks the sample processing liquid from flowing between the different drainage channels, but also makes the sample processing liquid evenly distributed in the different drainage channels, ensuring the correctness of the test results of different channels and the sensitivity of the test results. Moreover, the above-mentioned drainage grooves and drainage sheets can also effectively fix the upper and lower shells of the test reagent card together, so that the test reagent card provided by the present application does not need to be provided with a clamping structure for fixing the upper and lower shells together near the drainage channel, thereby reducing the size of the test reagent card and realizing the miniaturization of the test reagent card.

[0044] 3. In the hazard factor detection reagent card provided herein, the drainage plate and drainage groove are connected by an interference fit. This interference fit secures the drainage plate and drainage groove together, effectively preventing cross-flow between adjacent drainage channels and preventing backflow reagents from disrupting the flow between adjacent drainage channels. It also more securely secures the upper and lower housings.

[0045] 4. The hazard factor detection reagent card provided in the present application also includes: a rivet structure respectively arranged on one of the upper shell and the lower shell, and a ring groove structure arranged on the other of the upper shell and the lower shell; the rivet structure and the ring groove structure are plugged in and connected to fix the upper shell and the lower shell together.

[0046] In the present application, the upper shell and the lower shell can be effectively fixed together by the above-mentioned mutually cooperating rivet structure and ring groove structure, while ensuring the firmness of the connection between the drainage plate and the drainage groove.

[0047] 5. The hazard factor detection reagent card provided in the present application also includes: a wide-view detection window, through which the operator can conveniently observe the chromatography status of the detection reagent strip and the final test results.

[0048] 6. The hazard factor detection reagent card provided herein has a baffle on the lower housing for separating the detection reagent strips, and a fixing post on the upper housing for positioning the detection reagent strips. In this application, the baffle and fixing post effectively position and secure the detection reagent strips within each drainage channel, thereby preventing displacement of the detection reagent strips that could affect detection speed and accuracy.

[0049] 7. The hazard factor detection method provided herein utilizes the aforementioned hazard factor detection reagent card by placing a detection reagent strip for detecting large targets in the short-distance drainage channel, while simultaneously placing a detection reagent strip for detecting small targets in the long-distance drainage channel. This arrangement effectively ensures relatively high sensitivity in multi-channel detection results, guaranteeing both detection speed and accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the specific implementation methods of the present application or the technical solutions in the prior art, the following is a brief introduction to the drawings required for use in the specific implementation methods or the description of the prior art. Obviously, the drawings described below are some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0051] FIG1 is a schematic diagram of the three-dimensional structure of a ten-channel lower housing with a wide-view detection window in Example 1 provided by the present application;

[0052] FIG2 is a schematic diagram of the three-dimensional structure of a ten-channel upper housing with a wide-view detection window in Example 1 provided by the present application;

[0053] FIG3 is a schematic diagram of the relative positions of the smooth bend section and the connecting section in the drainage channel provided by the present application;

[0054] FIG4 is a schematic diagram of the three-dimensional structure of a sixteen-channel lower housing with a wide-view detection window in Example 2 provided by the present application;

[0055] FIG5 is a schematic diagram of the three-dimensional structure of the sixteen-channel upper shell with a wide-view detection window in Example 2 provided in the present application.

[0056] Explanation of the accompanying reference numerals: 1-sample loading port; 2-drainage platform; 3-long-distance drainage channel; 4-short-distance drainage channel; 5-upper shell; 6-lower shell; 7-drainage groove; 8-drainage plate; 9-rivet structure; 10-ring groove structure; 11-wide-view detection window; 12-smooth bend section; 13-connecting section; 14-baffle; 15-fixing column. DETAILED DESCRIPTION

[0057] The following will clearly and completely describe the technical solution of this application in conjunction with the accompanying drawings. Obviously, the embodiments described are only part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of this application.

[0058] Example 1

[0059] A hazard factor detection reagent card, which is a hazard factor detection reagent card based on biological probe multi-channel immunochromatography, as shown in Figures 1 and 2, comprises:

[0060] The outer shell comprises an upper shell 5 and a lower shell 6 which are assembled and connected;

[0061] Sample port 1, used for adding samples;

[0062] Drainage stations 2, there are 10 drainage stations 2, and each drainage station 2 is a detection channel for accommodating a separate detection reagent strip;

[0063] Drainage channels, there are 10 drainage channels, each of which independently connects the sample injection port 1 and the drainage platform 2; the drainage channels include: a long-distance drainage channel 3 and a short-distance drainage channel 4; the length of the long-distance drainage channel 3 connecting the sample injection port 1 and the drainage platform 2 is greater than that of the short-distance drainage channel 4; the detection reagent strip for detecting small-sized toxin targets is set on the drainage platform 2 corresponding to the long-distance drainage channel 3, the detection reagent strip for detecting larger-sized bacterial targets is set on the drainage platform 2 corresponding to the short-distance drainage channel 4, and the detection reagent strip for detecting viral targets between the two sizes is set on the drainage platform 2 corresponding to the drainage channel between the long-distance drainage channel 3 and the short-distance drainage channel 4. As shown in Figures 1 and 3, the drainage channels include: a drainage groove 7 set on the lower shell 6, and a drainage plate 8 set on the upper shell 5; when the outer shell is assembled, the drainage plate 8 is inserted and fixed in the drainage groove 7. The drainage groove 7 and drainage piece 8 connected by the above-mentioned plug-in connection effectively prevent the sample solution from flowing out from the gap between the different drainage channels between the upper and lower shells. It can not only effectively prevent the sample solution from flowing between different drainage channels and the turbulence after the backflow of the sample solution, but also make the sample processing liquid in different drainage channels evenly distributed, and ensure the accuracy of the detection results of different channels and the sensitivity of the detection results. Moreover, the drainage groove 7 and drainage piece 8 can also effectively fix the upper shell 5 and the lower shell 6 of the detection reagent card together, so that the detection reagent card provided by the present application does not need to be provided with a clamping structure for fixing the upper and lower shells together near the drainage channel, thereby reducing the size of the detection reagent card. Moreover, in order to further improve the connection reliability of the drainage groove 7 and the drainage piece 8 and reduce the gap between them, the drainage piece 8 and the drainage groove 7 are connected by interference fit;

[0064] A wide-view detection window 11 is provided for observing the chromatography status and final test results of the test strip; there is one wide-view detection window 11 on the outer shell, and the wide-view detection window 11 is correspondingly arranged on the central axis of the sample injection port 1; the long-distance drainage channel 3 and the short-distance drainage channel 4 are both arranged opposite to the sample injection port 1;

[0065] The baffle 14 and the fixing column 15 are provided on the lower shell 6 to separate adjacent detection reagent strips; the fixing column 15 is provided on the upper shell 5 to position the detection reagent strips.

[0066] In this embodiment, as shown in Figures 1 and 2, in order to firmly and reliably fix the upper shell 5 and the lower shell 6 together, an annular groove structure 10 is provided on the lower shell 6, and a rivet structure 9 is provided on the upper shell 5. The above-mentioned rivet structure 9 and the annular groove structure 10 are connected by plugging. Moreover, since the drainage channel in the present application is connected by assembling the above-mentioned drainage groove 7 and the drainage piece 8, the above-mentioned drainage groove 7 and the drainage piece 8 can firmly and reliably fix the lower shell 6 and the upper shell 5 together. Therefore, the above-mentioned rivet structure 9 and the annular groove structure 10 do not need to be provided around the drainage channel. It is only necessary to provide the rivet structure 9 and the annular groove structure 10 that are connected in a fitting manner on the side of the outer shell away from the drainage channel.

[0067] As shown in Figure 1, there is a schematic diagram of the three-dimensional structure of the lower shell with a wide-view detection window; the 10 drainage channels are arranged in the width direction of the outer shell; the sample loading port 1 is an elliptical structure extending along the width direction of the outer shell, and the long-distance drainage channel 3 and the short-distance drainage channel 4 have: a smooth bend section 12 extending toward the sample loading port 1, and a connecting section 13 extending toward the drainage table 2.

[0068] Since bacteria, viruses, and toxins are in a dispersed and suspended state in the sample solution, the chromatography process is affected by the pore size and self-resistance of the chromatography medium, such as the drainage pad, the sample pad on the detection reagent strip, the labeling pad, the analytical membrane, etc. The larger the size of the target, the greater the resistance and the longer the chromatography time. Under the same chromatography time, the actual reaction time of large-sized targets is relatively short. In order to ensure the synchronous chromatography of the targets to be detected on the detection reagent strips of each channel in the multi-channel detection reagent card, the detection reagent strip channels are allocated according to the target's own characteristics and the characteristics of the drainage structure. For example, compared with 50nm toxins and 2μm bacteria, the resistance they encounter is different due to their different sizes, which makes the 50nm toxin target chromatograph faster and the 2μm bacterial target chromatograph slower. Under the same chromatography time, the drainage structure is relied upon to complete the synchronization of the chromatography of targets of different sizes to each reagent strip. Therefore, 50nm toxin targets are distributed in drainage channels L1 to L3 and L8 to L10, which are farther from the sample injection point, as shown in Figure 1. 2μm bacterial target detection reagent strips are distributed in drainage channels L4 to L7, which are closer to the sample injection point, as shown in Figure 1. This setup effectively ensures the detection sensitivity of each target on the multi-channel detection reagent card.

[0069] In this embodiment, when virus detection is also required, to maintain multi-channel detection sensitivity while maintaining sensitivity, based on the above innovative structure and the size and movement of the analytes, virus detection reagent strips with a size of 20nm-200nm are placed in the L3 and L8 drainage channels as shown in Figure 1. Toxin detection reagent strips with a size of 1nm-100nm are placed in the L1 to L2 and L9 to L10 drainage channels. Bacteria detection reagent strips with a size of 0.5μm-5μm are placed in the L4 to L7 detection channels. Due to the larger size of anthrax spores (5μm-10μm), anthrax spore detection reagent strips are recommended to be placed in the L5 and L6 detection channels.

[0070] A method for detecting harmful factors comprises the following steps:

[0071] Step S1: Preparation of potent biohazard agent detection reagent strips and preparation of universal sample processing solution;

[0072] Step S2: Place the test strips for identifying different types of potent biohazard factors on different drainage platforms 2 of the multi-channel test reagent card, place drainage pads on the sample loading positions, and cover and tighten the upper cover;

[0073] Step S3: Add 1 mL to 3 mL of the sample prepared with the universal sample treatment solution to the sample injection port 1 and record the chromatography time to determine the detection time;

[0074] Step S4: inserting the reagent strip end of the reagent card into the detector to a fixed position;

[0075] Step S5: Start the detector to perform quantitative measurement on the test strip through the wide-view detection window 11, and the instrument displays the measurement results;

[0076] Step S6: All negative samples are added to the sample injection port 1 of the test reagent card. The results are read on the computer and each test reagent strip has a clear quality control peak and a very low test peak. The ratio of the test peak to the quality control peak shows a negative result.

[0077] Step S7: The sample to be tested prepared with the universal sample treatment solution is added to the sample well of the detection reagent card, and the result is read on the instrument. The ratio of the test peak value to the quality control peak value is regressed with the instrument's built-in standard curve to determine the result of a certain potent biohazard factor in the sample;

[0078] Wherein, in step S2:

[0079] The detection reagent strip for detecting large-sized targets is placed on the short-distance drainage channel 4, and the detection reagent strip for detecting small-sized targets is placed on the long-distance drainage channel 3;

[0080] Among them, the toxin detection reagent strip, the virus detection reagent strip and the bacteria detection reagent strip are sequentially arranged on the channels from long distance to short distance in the short distance drainage channel 4 and the long distance drainage channel 3 .

[0081] Of course, this embodiment does not specifically limit the location of the drainage groove 7 and the drainage plate 8 that constitute the drainage channel. In other embodiments, the drainage channel includes: the drainage groove 7 arranged on the upper shell 5, and the drainage plate 8 arranged on the lower shell 6.

[0082] Of course, this embodiment does not specifically limit the locations of the rivet structure 9 and the annular groove structure 10. In other embodiments, in order to firmly and reliably fix the upper shell 5 and the lower shell 6 together, the present application provides a rivet structure 9 on the upper shell 5 and a annular groove structure 10 on the lower shell 6. The rivet structure 9 and the annular groove structure 10 are connected by plugging.

[0083] Example 2

[0084] A hazard factor detection reagent card, which is a hazard factor detection reagent card based on biological probe multi-channel immunochromatography, as shown in Figures 4 and 5, comprising:

[0085] The outer shell comprises an upper shell 5 and a lower shell 6 which are assembled and connected;

[0086] Sample port 1, used for adding samples;

[0087] Drainage stations 2, there are 16 drainage stations 2, and each drainage station 2 is a detection channel for accommodating a separate detection reagent strip;

[0088] A wide-view detection window 11 is provided for observing the chromatography status and final test results of the test strip; there is one wide-view detection window 11 on the outer shell, and the wide-view detection window 11 is correspondingly arranged on the central axis of the sample injection port 1; the long-distance drainage channel 3 and the short-distance drainage channel 4 are both arranged opposite to the sample injection port 1;

[0089] Drainage channels, there are 16 drainage channels, each of which independently connects the sample addition port 1 and the drainage platform 2; the drainage channels include: a long-distance drainage channel 3 and a short-distance drainage channel 4; the length of the long-distance drainage channel 3 connecting the sample addition port 1 and the drainage platform 2 is greater than that of the short-distance drainage channel 4.

[0090] In this embodiment, to achieve multi-channel detection without sacrificing sensitivity, based on the above innovative structure and the principle of analyte size and movement, toxin detection strips with a size of 1nm-100nm are allocated to detection channels L1 to L3, L14 to L16, and L7 to L10 as shown in Figure 4. Virus detection strips with a size of 20nm-200nm are allocated to detection channels L4 and L5, L12, and L13. Bacteria detection strips with a size of 0.5μm-5μm are allocated to detection channels L6 to L11. Due to the larger size of anthrax spores, ranging from 5μm to 10μm, anthrax spore detection strips are recommended to be placed in detection channels L8 and L9.

[0091] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A hazard factor detection reagent card, which is a hazard factor detection reagent card based on biological probe multi-channel immunochromatography, characterized in that: include: outer shell; A sample injection port (1) for injecting samples; Drainage platforms (2), the number of the drainage platforms (2) is 2 to 20, and the drainage platforms (2) are used to accommodate separate detection reagent strips; Drainage channels, the number of which ranges from 2 to 20, each of which and the drainage pad independently connect the sample loading port (1) and the drainage platform (2); the drainage channels include: a long-distance drainage channel (3) and a short-distance drainage channel (4); The length of the long-distance drainage channel (3) connecting the sample addition port (1) and the drainage platform (2) is greater than that of the short-distance drainage channel (4); a detection reagent strip for detecting small-sized targets is arranged on the drainage platform (2) corresponding to the long-distance drainage channel (3), and a detection reagent strip for detecting large-sized targets is arranged on the drainage platform (2) corresponding to the short-distance drainage channel (4).

2. The hazard factor detection reagent card according to claim 1, characterized in that: The outer shell comprises: an upper shell (5) and a lower shell (6) which are assembled and connected; The drainage channel comprises: a drainage groove (7) provided on one of the upper shell (5) and the lower shell (6), and a drainage plate (8) provided on the other of the upper shell (5) and the lower shell (6); When the outer shell is in an assembled state, the drainage piece (8) is inserted and fixed in the drainage groove (7).

3. The hazard factor detection reagent card according to claim 2, characterized in that: The drainage piece (8) and the drainage groove (7) are connected by interference fit and plug-in connection.

4. The hazard factor detection reagent card according to claim 2, characterized in that: Also includes: a rivet structure (9) respectively provided on one of the upper shell (5) and the lower shell (6), and a ring groove structure (10) provided on the other of the upper shell (5) and the lower shell (6); The rivet structure (9) and the annular groove structure (10) are plugged together to fix the upper shell (5) and the lower shell (6) together.

5. The hazard factor detection reagent card according to claim 4, characterized in that: The rivet structure (9) and the annular groove structure (10) that are connected in a coordinated manner are arranged on a side of the outer shell away from the drainage channel.

6. The hazard factor detection reagent card according to any one of claims 2 to 5, characterized in that: The plurality of drainage channels are arranged in the width direction of the outer shell; The sample addition port (1) is an elliptical structure extending along the width direction of the outer shell, and the long-distance drainage channel (3) and / or the short-distance drainage channel (4) have: a smooth curved section (12) extending toward the sample addition port (1), and a connecting section (13) extending toward the drainage platform (2).

7. The hazard factor detection reagent card according to claim 6, characterized in that: Also includes: A wide-view detection window (11) is used to observe the chromatography status of the detection reagent strip and the final detection result.

8. The hazard factor detection reagent card according to claim 7, characterized in that: There is one wide-view detection window (11) on the outer shell, and the wide-view detection window (11) is correspondingly arranged on the central axis of the sample injection port (1); the long-distance drainage channel (3) and the short-distance drainage channel (4) are both arranged opposite to the sample injection port (1); or, There are two wide-view detection windows (11) on the outer shell, and the two wide-view detection windows (11) are symmetrically arranged on both sides of the sample addition port (1); the long-distance drainage channel (3) and the short-distance drainage channel (4) are arranged on both sides of the sample addition port (1).

9. The hazard factor detection reagent card according to claim 2, characterized in that: The lower housing (6) is provided with a baffle (14) for separating the detection reagent strips, and the upper housing (5) is also provided with a fixing column (15) for positioning the detection reagent strips.

10. A method for detecting hazardous factors, using the hazardous factor detection reagent card according to any one of claims 1 to 9, characterized in that: include: Step S1: Preparation of potent biohazard agent detection reagent strips and preparation of universal sample processing solution; Step S2: Place the test strips for identifying different types of potent biohazard factors on different drainage platforms (2) of the multi-channel test reagent card, place drainage pads on the sample loading positions, and cover and tighten the upper cover; Step S3: Add 1 mL to 3 mL of the sample prepared with the universal sample treatment solution to the sample injection port (1), and record the chromatography time to determine the detection time; Step S4: inserting the reagent strip end of the reagent card into the detector to a fixed position; Step S5: Start the detector to perform quantitative measurement on the test strip through the wide-view detection window (11), and the instrument displays the measurement results; Step S6: Add all negative samples to the sample injection port (1) of the test reagent card, and read the results on the machine. Each test reagent strip has a clear quality control peak and a very low test peak. The ratio of the test peak to the quality control peak shows a negative result. Step S7: Add the test sample prepared with the universal sample treatment solution to the sample injection port of the test reagent card, and read the results on the instrument. The ratio of the test peak value to the quality control peak value is regressed with the instrument's built-in standard curve to determine the result of a certain potent biohazard factor in the sample; Wherein, in step S2: A detection reagent strip for detecting large-sized targets is arranged on the short-distance drainage channel (4), and a detection reagent strip for detecting small-sized targets is arranged on the long-distance drainage channel (3); The toxin detection reagent strip, the virus detection reagent strip and the bacteria detection reagent strip are sequentially arranged on the channels from long distance to short distance in the short distance drainage channel (4) and the long distance drainage channel (3).

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