Flow nanocytometry devices and methods for nanoparticle detection

Abstract

A flow nanocytometer for measuring extracellular vesicles (EVs) may include a flow cell for receiving a core stream carry at least one EV, at least one excitation source module for outputting a light beam to interrogate a portion of the core stream, a detection module for detecting the light beam, and a processor communicatively coupled to a memory, the at least one excitation source, and the detection module. The processor may execute a method including receiving a plurality of electronic signals from the detection module; detecting an intensity of a polycystic kidney disease (PKD) associated protein expressed by the EVs based on the plurality of electronic signals; determining a quantity of the EVs based on the plurality of electronic signals; and determining a severity or a prognosis of polycystic kidney disease in the subject, based on the intensity of the PKD associated protein and the quantity of the EVs.

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