Sweet proteins and food products comprising the same

Abstract

The present disclosure relates to a food product comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

Description

[0001] SWEET PROTEINS AND FOOD PRODUCTS COMPRISING THE SAME

[0002] TECHNOLOGICAL FIELD

[0003] The present disclosure related to sweet proteins and food products comprising the same.

[0004] BACKGROUND

[0005] Dietary supplements and nutraceutical compositions have gained significant attention in recent years due to their potential health benefits, including improved nutrition, enhanced immunity, and the management of various chronic conditions.

[0006] GENERAL DESCRIPTION

[0007] In accordance with some aspects, the present disclosure provides a food product comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0008] In accordance with some aspects, the present disclosure provides a dietaiy supplement comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0009] In accordance with some aspects, the present disclosure provides a functional food comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0010] In accordance with some aspects, the present disclosure provides a gummy candy comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0011] In accordance with some aspects, the present disclosure provides a chewable vitamin comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0012] In accordance with some aspects, the present disclosure provides a liquid comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein. In accordance with some aspects, the present disclosure provides a carbonated energy drink comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0013] In accordance with some aspects, the present disclosure provides a protein bar and / or an energy bar and / or snack bar comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0014] In accordance with some aspects, the present disclosure provides a sport nutrition drink comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0015] In accordance with some aspects, the present disclosure provides a protein milk drink comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0016] In accordance with some aspects, the present disclosure provides an oral film strip comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0017] In accordance with some aspects, the present disclosure provides an electrolyte powder comprising one or more bioactive compounds and one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0018] BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to better understand the subject matter that is disclosed herein and to exemplify how it may be carried out in practice, embodiments will now be described, by way of non-limiting example only, with reference to the accompanying drawings, in which:

[0020] Figure 1 is a spider plot showing sensory profile of a gummy candy with 50% sugar reduction compared to gummy candy with 50% sugar reduction with sweet protein DETAILED DESCRIPTION

[0021] The present disclosure is based on the finding that sweet proteins can be effectively utilized in the preparation of various nutraceutical products, particularly in sugar-reduced functional foods and dietary supplements.

[0022] Nutraceutical products such as dietary supplements and functional foods, typically include functional ingredients including, inter alia, vitamins, minerals, and / or herbal extracts, are formulated to support overall well-being and address specific health concerns.

[0023] One of the challenges in the formulation of many of these products is the presence of added sugars. Sugar is commonly used in such products to improve taste, improve texture, and increase consumer acceptability, including, for example, in chewable tablets, gummies, syrups, and flavored powders. This may result in a situation in which people while seeking health benefits from supplements may unknowingly consume high amounts of sugar, counteracting the intended positive effects of these products and lead to health risks, such as obesity, diabetes, metabolic disorders, and dental problems.

[0024] The use of proteins and specifically sweet proteins presents an advantage in the preparation of these products.

[0025] Initially, the growing awareness of sugar-related health risks has driven demand forlow-sugar or sugar-free dietary supplements. However, reducing or eliminating sugar while maintaining product stability, palatability, taste and efficacy poses significant formulation challenges.

[0026] The use of sweet proteins as shown here successfully addresses this concern as the addition of sweet proteins results in a nutraceutical product that is characterized by reduced sugar as compared to the full-sugar counterpart nutraceutical product without the sweet proteins. It should be noted that the reduction of sugar achieved by addition of sweet protein(s) may be also significant even if the counterpart nutraceutical product is a reduced sugar product by itself. In addition, the addition of sweet protein(s) can be used in order to replace sugar alternatives in the counterpart nutraceutical product while maintaining a similar level of sweetness.

[0027] In addition, due to the high sweetness intensity of the sweet proteins, small quantities of the protein may be added in order to achieve the desired level of sweetness, thereby leaving more space within the product for other beneficial ingredients including, inter alia, vitamins or minerals. This efficient use of space enables products to pack a higher concentration of essential nutrients and bioactive compounds into the product, maximizing its nutritional value and potential health benefits for consumers.

[0028] Many nutraceutical products, including functional foods such as beverages, confections, or dietary supplements, traditionally rely on sucrose for sweetness, texture, and bulk. However, reducing sugar content through the incorporation of sweet proteins presents both advantages and challenges. As noted herein, sugar reduction may enhance the nutritional profile and facilitate the incorporation of additional bioactive compounds.

[0029] The present invention provides optimized formulation and method for sugar reduction in functional foods and supplementary food while maintaining desirable sensory, structural, and functional characteristics.

[0030] Hence, the present disclosure provides, in accordance with some aspects, a nutraceutical product comprising one or more sweeteners, wherein the one or more sweeteners is or comprises at least one sweet protein.

[0031] In the following text, when referring to the nutraceutical product it is to be understood as also referring to the functional food, the dietary supplement, and the method disclosed herein. Thus, whenever providing a feature with reference to the nutraceutical product, it is to be understood as defining the same feature with respect to the functional food, the dietary supplement, and the method mutatis mutandis.

[0032] The term "nutraceutical product", also referred to as "nutraceutical" , as used herein, refers to a composition / formulation that provides a physiological or health benefit beyond basic nutrition, including but not limited to dietary supplements and functional foods.

[0033] As described herein, the present disclosure is not limited to a specific nutraceutical product and is applicable to a variety of food products having beneficial effects.

[0034] As described herein, the beneficial effects is attributed to a bioactive compound incorporated in the nutraceutical product.

[0035] Hence, in accordance with some aspects, the present disclosure provides a food product comprising at least one sweet protein and one or more bioactive compounds. It should be noted that in accordance with the present disclosure, reference to a food product should be understood to a food product comprising at least one sweet protein and one or more bioactive compound.

[0036] In some embodiments, the food product is one or more of a dietary supplement or a functional food.

[0037] In some embodiments, the food product is a dietary supplement.

[0038] The term "dietary supplement" refers to a product that comprises a dietaiy ingredient intended to supplement the diet. Dietary supplements may be in the form of a tablet, a capsule, a powder, or liquid form with specific dosage recommendations.

[0039] In some embodiments, the food product is a functional food.

[0040] The term "functional food" refers to a food product that provides health benefits beyond basic nutrition, either by containing naturally occurring bioactive compounds or through fortification / enrichment during processing. Functional foods include, but are not limited to, sports nutrition drinks, fortified beverages, protein bars, and probioticcontaining dairy products.

[0041] The food product described herein, i.e. a functional food and / or a dietaiy supplement may comprise one or more bioactive compound / substance.

[0042] The term "bioactive compound" or "bioactive substance", as used herein, refers to a naturally occurring or synthetically derived compound that has a physiological effect on the human body when consumed. Bioactive compounds typically provide health benefits beyond basic nutrition, for example, enhancing metabolism, supporting immune function, improving digestion, or reducing inflammation.

[0043] The present disclosure is not limited to a specific bioactive compound and is applicable for any compound intended to support well-being and health maintenance.

[0044] In some embodiments which may be considered as aspects of the present disclosure, the food product is a functional food comprising at least one sweet protein and one or more bioactive compounds

[0045] In some embodiments which may be considered as aspects of the present disclosure, the food product is a dietary supplement comprising at least one sweet protein and one or more bioactive compounds The selection, concentration, and combination of bioactive compounds in the products described herein may be optimized based on the intended health benefit, formulation stability, and regulatory considerations.

[0046] In some examples, the bioactive compound is one or more of bioactive compound is or comprises one or more of a vitamin, a mineral, a botanical extract, a phytochemical, an amino acid, a probiotic, a prebiotic, a postbiotic, a peptide, a protein, a nucleotide, a nucleoside, a polyphenol, a flavonoid, a carotenoid, an alkaloid, a glycoside, a lipid, an essential fatty acid, an antioxidant, an enzyme, a metabolite, an electrolyte, a stimulant, a fiber or any combination thereof. It should be noted that when referring for example to "one or mor of a vitamin" it encompasses also one or more vitamins.

[0047] In some examples, the bioactive compound is one or more of a vitamin, a mineral, a botanical extract, a phytochemical, an amino acid, a probiotic, a prebiotic, a postbiotic, a peptide, a protein, a nucleotide, a nucleoside, a polyphenol, a flavonoid, a carotenoid, an alkaloid, a glycoside, a lipid, an essential fatty acid, an antioxidant, an enzyme, a metabolite, an electrolyte, a stimulant, a coenzyme, a microbial metabolite, or any combination thereof.

[0048] In some examples, the bioactive compound is one or more of a vitamin, a mineral, a botanical extract, a phytochemical, an amino acid, a probiotic, a prebiotic, postbiotics, a lipid, an essential fatty acid, an antioxidant, an enzyme, a metabolite, an electrolyte, a stimulant, or any combination thereof.

[0049] In some examples, the bioactive compound is one or more of a vitamin, a mineral, an antioxidant, caffeine, taurine, a fiber or any combination thereof.

[0050] The present disclosure is not limited to a specific vitamin and the food product, being a functional food and / or a dietary supplement, may comprise one or more different vitamins.

[0051] In some examples, the food product comprises one or more vitamins.

[0052] In some examples, the food product comprises one or more of vitamin C, vitamin B, vitamin A, vitamin D, vitamin E, vitamin K or any combination thereof.

[0053] In some examples, the food product comprises vitamin C and / or B vitamins.

[0054] In some examples, the food product comprises vitamin C. In some examples, the food product comprises vitamin B.

[0055] In some examples, vitamin B is one or more of vitamin B 1 (Thiamine), vitamin B2 (Riboflavin), vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin B6 (Pyridoxine), vitamin B7 (Biotin), vitamin B9 (Folic Acid), vitamin B 12 (Cobalamin) or any combination thereof.

[0056] In some examples, vitamin B is one or more of vitamin B 1 (Thiamine), vitamin B2 (Riboflavin), vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin B6 (Pyridoxine), vitamin B7 (Biotin), vitamin B8, vitamin B9 (Folic Acid), vitamin B12 (Cobalamin) or any combination thereof.

[0057] In some examples, the vitamin is vitamin B9 (Folic Acid).

[0058] In some examples, the vitamin is vitamin C, B-complex vitamin, vitamin A, vitamin D, vitamin E, or vitamin K. In some examples, the vitamin is vitamin C.

[0059] The food product described herein may comprise one or more minerals. The present disclosure is not limited to a specific mineral.

[0060] In some examples, the food product comprises one or more minerals. In some examples, the one or more minerals is a mineral salt.

[0061] In some examples, the one or more minerals is a magnesium salt, a potassium salt, a calcium salt, a sodium salt or any combination thereof.

[0062] In some examples, the one or more minerals is magnesium citrate, potassium citrate, calcium carbonate, sodium chloride or any combination thereof.

[0063] In some examples, the one or more minerals is magnesium citrate.

[0064] In some examples, the one or more minerals is calcium carbonate.

[0065] In some examples, the one or more minerals is potassium citrate. In some examples, the one or more minerals is tri potassium citrate.

[0066] In some examples, the mineral is an essential mineral.

[0067] In some examples, the mineral is one or more of magnesium, calcium, potassium, zinc, iron, selenium or any combination thereof.

[0068] In some examples, the mineral is potassium. In some examples, the mineral is iron. The food product described herein may comprise one or more botanical extracts. The botanical extract referred to any component extracted from a plant material. The plant material is one or more of roots, leaves, berries, seeds or any combination thereof.

[0069] In some examples, the botanical extract is an extract of a green tea extract, a turmeric extract, a ginseng extract, an ashwagandha extract, an echinacea extract, an elderberry extract, a grapeseed extract, a milk thistle extract, a maca root extract, a rhodiola extract or any combination thereof.

[0070] In some examples, the botanical extract comprises one or more of catechin (EGCG), curcuminoid (curcumin), ginsenoside, withanolide, alkamide, caffeic acid derivative, anthocyanin, flavonoid, proanthocyanidin, silymarin (flavonolignans), macamide, alkaloid, rosavin, salidroside or any combination thereof.

[0071] In some examples, the phytochemical is a plant-derived compound. In some examples, the plant derived compound is a polyphenol, a carotenoid, an alkaloid, or any combination thereof. In some examples, the polyphenol is one or more of a flavonoid, a catechin, an anthocyanin, a resveratrol, a quercetin or any combination thereof. In some examples, the carotenoid is one or more of a beta-carotene, lutein, lycopene, zeaxanthin or any combination thereof. In some examples, the alkaloid is one or more of caffeine and / or theobromine.

[0072] In some examples, the amino acid is an amino acid or derivative thereof. In some examples, the amino acid derivative is taurine.

[0073] In some examples, the probiotic is one or more of lactobacillus, bifidobacterium, saccharomyces boulardii or any combination thereof.

[0074] In some examples, the prebiotic is a fiber. In some examples, the bioactive compound is a fiber.

[0075] The term "fiber" as used herein refers to dietary fiber, primarily plant-based dietary fiber. The dietary fiber as used herein may be considered as such that resist digestion by human gastrointestinal enzymes.

[0076] In some embodiments, the at least one fiber is at least one dietary fiber. The term dietary fibers as used herein encompasses soluble fibers and insoluble fibers. In some embodiments, the at least one dietary fiber is a water-soluble fiber. The term water- soluble fiber refers to fibers that are capable of dissolving in water and forming a gel-like substance.

[0077] The dietary fiber may be categorized based on the number of the monomeric unit composing the fiber.

[0078] In some embodiments, the dietary fiber comprises at most three monomeric units, at times at most five, at times at most ten monomeric units. In some embodiments, the dietary fiber comprises at least ten monomeric units.

[0079] In some embodiments, the dietary fiber comprises one or more non-starch polysaccharides, resistant starch (RS) and resistant oligosaccharides. In some embodiments, the dietary fiber comprises one or more non-starch polysaccharides.

[0080] In some embodiments, the non-starch polysaccharide is or comprises one or more of cellulose, hemicellulose, gum, pectin, mucilage, inulin, psyllium, P-glucan or any combination thereof.

[0081] In some examples the fiber is pectin. In some examples, the at least one dietaiy fiber is pectin. In some embodiments, the dietary fiber comprises one or more RS. In some embodiments, the dietary fiber comprises one or resistant oligosaccharides. In some embodiments, the resistant oligosaccharide is or comprises one or more of galactooligosaccharide (GOS), fructo-oligosaccharide (FOS), inulin or any combination thereof. In some examples, the fiber is inulin, a fructooligosaccharide (FOS), a galactooligosaccharides (GOS) or any combination thereof. In some examples, the fiber is FOS. In some examples, the fiber is GOS.

[0082] In some examples, the fiber is inulin. In some examples, the at least one dietaiy fiber is inulin. Inulin as used herein refers to a fiber typically derived from chicory root. The amount of at least one fiber in the food product may be adjusted according to specific needs and characteristics of the food product.

[0083] In some examples, the postbiotics is a short -chain fatty acids (SCFAs)).

[0084] In some examples, the lipid is a fatty acid, a phospholipid, a sterol, a plant sterol, a triglyceride or any combination thereof. In some examples, the lipid is an amino acid or derivative thereof. In some examples, the nutraceutical product comprises taurine. In some examples, the essential fatty acid is one or more of omega-3 fatty acid, and / or omega-6 fatty acid.

[0085] In some examples, the omega-3 fatty acids is Eicosapentaenoic acid (EP A), Docosahexaenoic acid (DHA) or Alpha-linolenic acid (ALA).

[0086] In some examples, the omega-6 fatty acids is linoleic acid or gamma-linolenic acid.

[0087] In some examples, the antioxidants is an anti-inflammatory compound.

[0088] In some examples, the antioxidants is Coenzyme Q10 (CoQlO), curcumin, astaxanthin, lutein, and lycopene.

[0089] In some examples, the enzyme is one or more of amylase, lipase, protease, lactase, bromelain, papain, cellulase, or invertase.

[0090] In some examples, the metabolite is one or more of inositol, creatine, L-camitine, coenzyme Q10 (CoQlO), nicotinamide adenine dinucleotide (NAD+), beta-hydroxy betamethylbutyrate (HMB), or short-chain fatty acids (SCFAs). In some examples, the metabolite is inositol.

[0091] In some examples, the electrolyte is one or more of sodium, potassium, magnesium, calcium, chloride, phosphate, or bicarbonate

[0092] In some examples, the stimulant is one or more of caffeine, theobromine, theophylline, guarana extract, green tea extract, yerba mate extract, or synephrine. In some examples, the stimulant is caffeine.

[0093] In some examples, the bioactive compound is one or more proteins.

[0094] In some examples, the bioactive compound is one or more of vitamin Bl (Thiamine), vitamin B2 (Riboflavin), vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin B6 (Pyridoxine), vitamin B7 (Biotin), vitamin B8, vitamin B9 (Folic Acid), vitamin B12 (Cobalamin), vitamin C, a magnesium salt, a potassium salt, a calcium salt, a sodium salt, iron, FOS, GOS, inositol, caffeine, taurine, fiber, a protein, or any combination thereof.

[0095] In some examples, the bioactive compound is one or more of a fiber, a magnesium salt, a potassium salt, iron, vitamin C, vitamin B, vitamin B9, inositol or any combination thereof. In some examples, the bioactive compound is one or more of FOS, GOS, vitamin C, vitamin Bl, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, vitamin B 12 or any combination thereof.

[0096] In some examples, the bioactive compound is one or more of a calcium salt, a magnesium salt, a potassium salt, vitamin C, B vitamins, vitamin B9 or any combination thereof. In some examples, the bioactive compound is one or more of calcium carbonate, magnesium citrate, tripotassium citrate, vitamin C, B vitamins, vitamin B9 or any combination thereof.

[0097] In some examples, the bioactive compound is one or more of magnesium citrate, vitamin C, vitamin, caffeine, taurine or any combination thereof.

[0098] In some examples, the bioactive compound is one or more of taurine, a potassium salt, a calcium salt, a magnesium salt, caffeine, vitamin B, or any combination thereof. In some examples, the bioactive compound is one or more of taurine, tri potassium citrate, calcium carbonate, caffeine, vitamin B3, vitamin B5, vitamin B6, vitamin B2, magnesium citrate, vitamin B l, vitamin B9, vitamin B12 or any combination thereof.

[0099] In some examples, the bioactive compound is one or more of calcium carbonate, vitamin C, taurine, vitamin B 1 (Thiamine), vitamin B2 (Riboflavin), vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin B6 (Pyridoxine), vitamin B9 (Folic Acid), vitamin B12 (Cobalamin), magnesium carbonate, tri potassium citrate or any combination thereof.

[0100] In some examples, the bioactive compound is one or more of magnesium citrate, tri potassium citrate, iron, vitamin C, vitamin B9, inositol, taurine or any combination thereof.

[0101] In some examples, the bioactive compound is one or more of a whey protein, taurine, tri potassium citrate, calcium carbonate, vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin Bl (Thiamine), vitamin B 6 (Pyridoxine), magnesium citrate, vitamin B9 (Folic Acid), vitamin B 12 (Cobalamin), or any combination thereof.

[0102] In some examples, the bioactive compound is one or more of a whey protein, sodium, calcium, magnesium, potassium iron, vitamin C, vitamin B, vitamin B9 (Folic Acid), inositol, taurine or any combination thereof.

[0103] In some examples, the bioactive compound is one or more of a whey protein, tripotassium citrate, calcium carbonate, sodium chloride, vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), magnesium citrate, vitamin B6 (Pyridoxine), vitamin B2 (Riboflavin), vitamin Bl (Thiamine), vitamin B9 (Folic Acid), vitamin B12 (Cobalamin), or any combination thereof.

[0104] The present disclosure is not limited to a specific protein. In some examples, the protein is a sweet protein.

[0105] In some embodiments, the sweet protein is identical to a naturally occurring protein or is a naturally occurring protein.

[0106] In some other embodiments, the sweet protein is found in plants such as tropical plants. Non-limiting examples of a plant include at least one of capparis masaikai, oobli, serendipity berry, katemfe, miracle fruit berry, or lemba.

[0107] In some embodiments, the sweet protein is selected from the group consisting of Monellin, Thaumatin, Miraculin, Curculin, Brazzein, Mabinlin, a sweet protein from honey truffle, or a variant thereof.

[0108] The term “varianf as used herein refers to a sequence that contains at least one amino acid modification (e.g. substitution, deletion, or insertion) as compared to a reference protein.

[0109] The present disclosure also encompasses a modified nucleic acid sequence, including corresponding substitution, deletion or insertion of codons similar to the corresponding substitution, deletion or insertion in the modified protein.

[0110] In some embodiments, the reference protein is selected from the group consisting of Monellin, Thaumatin, Miraculin, Curculin, Brazzein, a sweet protein from honey truffule, and Mabinlin.

[0111] In some embodiments, the sweet protein is Monellin. In some embodiments, the reference protein is Monellin. In some embodiments, Monellin comprises chain A Monellin (GenBank Entry No. P02881; denoted herein by SEQ ID NO:1) and chain B Monellin (GenBank Entry No. P02882; denoted herein by SEQ ID NO:2).

[0112] In some embodiments, the sweet protein is Thaumatin. In some embodiments, the sweet protein is Thaumatin-1 (GenBank Entry No. P02883; denoted herein by SEQ ID NO:3). In some embodiments, the sweet protein is Thaumatin-2 (GenBank Entry No. P02884; denoted herein by SEQ ID NO:4). In some embodiments, the sweet protein is Miraculin (GenBank Entry No. P13087; denoted herein by SEQ ID NO:5).

[0113] In some embodiments, the sweet protein is Curculin-1 GenBank Entry No. P19667; denoted herein by SEQ ID NO:6) or Curculin-2 (GenBank Entry No. Q6F495; denoted herein by SEQ ID NO:7).

[0114] In some embodiments, the sweet protein is Brazzein (also known as: Defensin-like protein) (GenBank Entry No. P56552; denoted herein by SEQ ID NO:8).

[0115] In some embodiments, the sweet protein is Mabinlin I / Sweet protein mabinlin-1 (GenBank Entry No. P80351; denoted herein by SEQ ID NO:9), Mabinlin II (also known as Sweet protein mabinlin-2) (GenBank Entry No. P30233; denoted herein by SEQ ID NO:10), Mabinlin III (also known as Sweet protein mabinlin-3) (GenBank Entry No. P80352; denoted herein by SEQ ID NO: 11), Mabinlin IV (also known as Sweet protein mabinlin-4) (GenBank Entry No. P80353; denoted herein by SEQ ID NO: 12) or mabinlin-1 chain A (GenBank Entry No. B9SA35; denoted herein by SEQ ID NO:13).

[0116] In some embodiments, the sweet protein is derived from the honey truffle, or Mattirolomyces terfezioides. In some embodiments, the sweet protein is a sweet truffle protein or my codulcein (denoted herein by SEQ ID NO:352).

[0117] In some embodiments, the sweet protein has an amino acid sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NOTO, SEQ ID NO:11, SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO:352.

[0118] In some embodiments, the sweet protein is or comprises one or more proteins denoted by SEQ ID NO:1, SEQ ID N0:2, SEQ ID N0:3, SEQ ID N0:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID N0:7, SEQ ID NO:8, SEQ ID NOV, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO:352, a variant thereof or any combinations thereof.

[0119] In some embodiments, the sweet protein is a non-natural protein.

[0120] In some embodiments, the sweet protein is a synthetic protein or an engineered protein. The synthetic sweet protein may comprise the entire or part of the amino acid sequence of the naturally occurring protein (all or part of the protein's polypeptide chains) or part thereof. For example, the sweet protein may comprise a bond modification of a naturally occurring protein resulting in single polypeptide chain which corresponds to a naturally occurring protein, such that the at least two polypeptide chains of the wild type protein are covalently attached by other amino acids.

[0121] In some embodiments, the sweet protein is a modified Monellin protein known as MNEI. In some embodiments, the sweet protein is MNEI or a variant thereof.

[0122] In some embodiments, the sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO:14 or SEQ ID NO:350). In some embodiments, the sweet protein is MNEI as denoted by SEQ ID NO: 14 or a variant thereof. In some embodiments, the sweet protein is MNEI as denoted by SEQ ID NO: 350 or a variant thereof. In some embodiments, the reference protein is MNEI as denoted by SEQ ID NO: 14 or by SEQ ID NO: 350.

[0123] In some embodiments, the sweet protein that is a variant of a MNEI protein (SEQ ID NO: 14 and / or by SEQ ID NO: 350) is characterized by a sweetness threshold of at most about 0.05 mg / ml, at times at most about 0.1 mg / ml, at times at most about 0.2 mg / ml, at times at most about 0.3 mg / ml, at times at most about 0.5 mg / ml, at times at most about 1 mg / ml, at times at most about 2 mg / ml, at times at most about 3 mg / ml, at times at most about 5 mg / ml.

[0124] In some embodiments, the sweet protein that is a variant of a MNEI protein is characterized by a sweetness threshold of about 0.01 mg / ml, at times about 0.03 mg / ml, at times about 0.05 mg / ml, at times about 0.1 mg / ml, at times about 0.2 mg / ml, at times about 0.3 mg / ml, at times about 0.5 mg / ml, at times about 1 mg / ml, at times about 2 mg / ml, at times about 3 mg / ml, at times about 5 mg / ml.

[0125] In some embodiments, the sweet protein that is a variant of a MNEI protein is characterized by a sweetness that is at least about 500 times as compared to sucrose, at least about 800 times as compared to sucrose, at least about 1000 times as compared to sucrose, at least about 1500 times as compared to sucrose, at least about 2000 times as compared to sucrose, at least about 3000 times as compared to sucrose, at least about 4000 times as compared to sucrose, at least about 5000 times as compared to sucrose, at least about 6000 times as compared to sucrose, at least about 7000 times as compared to sucrose, at least about 8000 times as compared to sucrose, at least about 9000 times as compared to sucrose, at least about 10000 times as compared to sucrose, at least about 11000 times as compared to sucrose, at least about 12000 times as compared to sucrose, at least about 13000 times as compared to sucrose, at least about 14000 times as compared to sucrose, at least about 15000 times as compared to sucrose.

[0126] In some embodiments, the sweet protein that is a variant of a MNEI protein is characterized by a thermostability of at least about 70°C, at times at least about 73°C, at times at least about 75°C, at times at least about 80°C, at least about 85°C, at times at least about 90°C, at times at least about 94°C, at times at least about 95°C, at times at least about 97°C, at times at least about 98°C, at times at least about 99°C, at times at least about 100°C, at times at least about 101°C, at times at least about 102°C, at times at least about 103°C, at times at least about 104°C, at times at least about 105°C.

[0127] In some embodiments, the sweet protein that is a variant of a MNEI protein is characterized by a thermostability of about 70°C, at times about 73°C, at times about 75°C, at times about 80°C, at times about 85°C, at times about 90°C, at times about 94°C, at times about 95°C, at times about 97°C, at times about 98°C, at times about 99°C, at times about 100°C, at times about 101°C, at times about 102°C, at times about 103°C, at times about 104°C, at times about 105°C.

[0128] Thermostability as used herein refers to a protein's ability to maintain one or more of structure and function at the recited temperature. Thermostability can be measured through various techniques known in the art. For example, thermostability can be measured by Differential Scanning Calorimetry (DSC) or Differential Scanning Fluorimetry (DSF).

[0129] In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO: 16 (denoted herein as DM08). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO: 17 (denoted herein as DM09). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:39 (denoted herein as DM31). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:53 (denoted herein as DM69). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO : 104 (denoted herein as DM137).

[0130] In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO: 186 (denoted herein as DM165). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:217 (denoted herein as DM685). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:263 (denoted herein as DM528). In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:257 (denoted herein as DM532).

[0131] In some embodiments, the sweet protein has an amino acid sequence denoted by SEQ ID NO:351 (denoted herein as DM170).

[0132] In some embodiments, the sweet protein is a variant of a MNEI protein (SEQ ID NO:14 and / or SEQ ID NO:350).

[0133] In some embodiments, the sweet protein is a variant of a protein denoted by SEQ NO : 14. In some embodiments, the sweet protein is a variant of a protein denoted by SEQ NO: 350.

[0134] In some embodiments, the reference protein is one or more proteins denoted by SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ ID NO: 186, SEQ ID NO :217, SEQ IDNO:263, SEQ IDNO:257, SEQ ID NO :351 or any combinations thereof.

[0135] In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO: 16 (denoted herein as DM08). In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO: 17 (denoted herein as DM09). In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO:39 (denoted herein as DM31). In some embodiments, the sweet protein is a variant of a sweet protein denoted by SEQ ID NO:53 (denoted herein as DM69). In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO: 104 (denoted herein as DM137).

[0136] In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO:186 (denoted herein as DM165). In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO:217 (denoted herein as DM685).

[0137] In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO:263 (denoted herein as DM528). In some embodiments, the sweet protein is a variant of a sweet protein denoted by SEQ ID NO:351 (denoted herein as DM170). In some embodiments, the sweet protein is a variant of a protein denoted by SEQ ID NO :257 (denoted herein as DM532).It should be noted that the recitation “the sweet protein is a variant of a protein, for example MNEI protein” should be understood such that the protein is includes modifications as described herein as compared to the reference protein, for example MNEI, being the reference protein.

[0138] The present disclosure is not limited to specific number of amino acid modifications made in the reference protein that eventually results in the formation of the protein described herein.

[0139] A modification as used herein refers to a modification in one or more amino acids of the reference protein and encompasses amino acid substitution (replacement), amino acid deletion, amino acid insertion or any combination thereof.

[0140] In some examples, a modification may be an amino acid insertion.

[0141] In some embodiments, the protein comprises an amino acid sequence having at least one, at least two, at least three, at least four, at least five amino acid insertions as compared to the reference protein.

[0142] In some examples, a modification may be an amino acid deletion.

[0143] In some embodiments, the sweet protein comprises an amino acid sequence having at least one, at least two, at least three, at least four, at least five amino acid deletions as compared to the reference protein.

[0144] In some examples, a modification may be an amino acid substitution.

[0145] In some embodiments, the sweet protein comprises an amino acid sequence having at least one, at least two, at least three, at least four, at least five amino acid substitutions as compared to the reference protein.

[0146] In some embodiments, the sweet protein is a variant of a reference protein including one or more of (i) one or more amino acids replacements, (ii) one or more amino acids deletions, (iii) one or more amino acids insertions or (iv) any combination thereof.

[0147] In some embodiments, the sweet protein is a modified reference protein including one or more amino acids replacements.

[0148] In some embodiments, the sweet protein is a modified reference protein including one or more amino acids deletions.

[0149] In some embodiments, the sweet protein is a modified reference protein including one or more amino acids insertions. In some embodiments, the sweet protein comprises one or more amino acid modifications as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO:14.

[0150] In some embodiments, the sweet protein comprises one or more amino acid modifications as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO:350.

[0151] In some embodiments, the sweet protein comprises one or more amino acid modifications as compared to one or more proteins denoted by SEQ ID NO: 16, SEQ ID NO:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ ID NO:186, SEQ ID NO:217, SEQ ID NO:263, SEQ ID NO:257, SEQ ID NO:351 or any combinations thereof.

[0152] In some embodiments, the sweet protein comprises one or more amino acid modifications in one or more residue G1,E2, E4, 18, Fl 1, T12, N14, G16, K17, F18, A19, V20, D21, E23, K25, 126, G27, Q28, R31, T33, N35, K36, C41, E50, F52, R53, E59, Q61, L62, V64, Y65, S67, D68, L70, A73, D74, 175, S76, E77, R84, F89 or any combination thereof as compared to the reference protein.

[0153] In some embodiments, the sweet protein comprises one or more amino acid replacement as compared to MNEI protein having an amino acid sequence denoted by SEQ ID N0:14 or SEQ ID NO:350. In some embodiments, the sweet protein comprises one or more amino acid replacements as compared to one or more proteins denoted by SEQ ID NO:16, SEQ ID NO:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ IDNO:186, SEQ IDNO:217, SEQ IDNO:263, SEQ IDNO:257, SEQ ID NO:351 or any combinations thereof.

[0154] In some embodiments, the sweet protein comprises one or more amino acid replacement in one or more residue G1,E2, E4, 18, Fl 1, T12, N14, G16, K17, F18, A19, V20, D21, E23, K25, 126, G27, Q28, R31, T33, N35, K36, C41, E59, Q61, L62, V64, Y65, S67, D68, L70, A73, D74, 175, S76, E77, R84, F89 or any combination thereof as compared to the reference protein, for example MNEI protein.

[0155] In some embodiments, the sweet protein comprises one or more amino acid deletions as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO: 14. In some embodiments, the sweet protein comprises one or more amino acid deletions as compared toMNEI protein having an amino acid sequence denoted by SEQ ID NO:350. In some embodiments, the sweet protein comprises one or more amino acid deletions as compared to one or more proteins denoted by SEQ ID NO: 16, SEQ ID NO:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ ID NO:186, SEQ ID NO:217, SEQ ID NO:263, SEQ ID NO:257, SEQ ID NO:351 or any combinations thereof.

[0156] In some embodiments, the sweet protein comprises one or more amino acid deletions in one or more amino acid E50, F52, R53 as compared to the reference protein.

[0157] In some embodiments, the sweet protein comprises one or more amino acid deletions in one or more amino acid E50, F52, R53 as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO: 14.

[0158] In some embodiments, the sweet protein comprises one or more amino acid deletions in one or more amino acid E50, F52, R53 as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO:350.

[0159] In some embodiments, the sweet protein comprises one or more amino acid replacements in one or more amino acid S76, E23, Q28, E2, Y65, D68 as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO: 14.

[0160] In some embodiments, the sweet protein comprises one or more amino acid replacements in one or more amino acid S76, E23, Q28, E2, Y65, D68 as compared to MNEI protein having an amino acid sequence denoted by SEQ ID NO:350.

[0161] In some embodiments, the sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 14- SEQ ID NO:351, SEQ ID NO :353 -SEQ ID NO:479 or any combination thereof.

[0162] In some embodiments, the sweet protein is any one of the proteins defined in Table 20 shown below. It should be noted that at times, each row in Table 20 represents a different embodiment of the present application.

[0163] It should be noted that the present disclosure encompasses a combination of two or more sweet proteins.

[0164] In some examples, the nutraceutical product comprises two or more sweet proteins. In some embodiments, nutraceutical product comprises two or more sweet proteins, at times three or more, at times four or more, at times five or more sweet proteins selected from SEQ ID NO:14- SEQ ID NO:351, SEQ ID NO:353-SEQ ID NO:479 or any combination thereof.

[0165] In some embodiments, nutraceutical product comprises two sweet proteins, at times three sweet proteins, at times four sweet proteins, selected from SEQ ID NO: 14- SEQ ID NO:351, SEQ ID NO:353-SEQ ID NO:479 or any combination thereof.

[0166] In some examples, the nutraceutical product comprises two or more sweet proteins as denoted in Table 20. In some examples, the nutraceutical product comprises two or more sweet protein from the list of sweet proteins denoted in Table 20.

[0167] In some examples, the nutraceutical product comprises a combination of two or more proteins, wherein one of the proteins is MNEI or a variant thereof.

[0168] In some examples, the protein comprises a combination of two or more proteins, wherein at least one of the two or more proteins is a protein denoted by one or more of SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO: 104, SEQ ID NO: 186, SEQ ID NO :217, SEQ IDNO:263, SEQ IDNO:257, SEQ ID NO:351 or any combinations thereof.

[0169] In some examples, the protein comprises a combination of three or more proteins, wherein at least one of the two or more proteins is a protein denoted by one or more of SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO: 104, SEQ ID NO: 186, SEQ ID NO :217, SEQ IDNO:263, SEQ IDNO:257, SEQ ID NO:351 or any combinations thereof.

[0170] In some examples, the protein comprises a combination of four or more proteins, wherein at least one of the two or more proteins is a protein denoted by one or more of SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO: 104, SEQ ID NO: 186, SEQ ID NO :217, SEQ IDNO:263, SEQ IDNO:257, SEQ ID NO:351 or any combinations thereof.

[0171] In some examples, the protein comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and one or more of a protein denoted by SEQ ID NO:217 (denoted herein as DM685), a protein denoted by SEQ ID NO:263 (denoted herein as DM528), a protein denoted by SEQ ID NOTCH (denoted herein as DM137), a protein denoted by SEQ ID NO: 17 (denoted herein as DM09), a protein denoted by SEQ ID NO: 351 (denoted herein as DM170), a protein denoted by SEQ ID NO:53 (denoted herein as DM69), a protein denoted by SEQ ID NO:186 (denoted herein as DM165), a protein denoted by SEQ IDNO:257 (denoted herein as DM532) or a combination thereof.

[0172] In some examples, the nutraceutical product comprises a combination of two or more sweet proteins, wherein at least one of the two or more proteins is denoted by SEQ ID N0:16, SEQ ID NO:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ ID NO:186, SEQ ID NO:217, SEQ ID NO:263, SEQ ID NO:257, SEQ ID NO:351 or any combinations thereof.

[0173] In some examples, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:217 (denoted herein as DM685).

[0174] In some embodiments, the sweet protein includes a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:263 (denoted herein as DM528).

[0175] In some embodiments, the sweet protein includes a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO: 104 (denoted herein as DM137).

[0176] In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO: 17 (denoted herein as DM09).

[0177] In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:357 (denoted herein as DM170).

[0178] In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:53 (denoted herein as DM69).

[0179] In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO: 186 (denoted herein as DM165). In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:257 (denoted herein as DM532)

[0180] In some embodiments, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31), a protein denoted by SEQ ID NO: 17 (denoted herein as DM09) and a protein denoted by SEQ ID NO: 186 (denoted herein as DM165).

[0181] In some examples, the nutraceutical product comprises a combination of two or more proteins, wherein one of the protein is MNEI or a variant thereof and one of the proteins is a protein denoted by SEQ ID NO:8.

[0182] In some examples, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NON.

[0183] In some examples, the nutraceutical product comprises a combination of a variant of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID NO:8.

[0184] In some examples, the nutraceutical product comprises a combination of two or more proteins, wherein one of the proteins is MNEI or a variant thereof and one of the proteins is a protein denoted by SEQ ID NO:3 or SEQ ID NON.

[0185] In some examples, the nutraceutical product comprises a combination of a protein denoted by SEQ ID NO:39 (denoted herein as DM31) and a protein denoted by SEQ ID SEQ ID NON or SEQ ID NON.

[0186] In some examples, the nutraceutical product comprises a combination of a variant of a protein denoted by SEQ ID NON 9 (denoted herein as DM31) and a protein denoted by SEQ ID NON or SEQ ID NON.

[0187] According to some embodiments, the sweet protein includes a combination of any one or more of the proteins set forth in SEQ ID NO: I to SEQ IDNON79 and a saccharide. According to some embodiments, the herein-disclosed saccharide may include monosaccharide, disaccharide, oligosaccharide, or polysaccharide. In some embodiments, the sweet protein includes a combination of any one or more of the MNEI variants (i.e., variants of SEQ ID NO: 14 or SEQ ID NO:350) and the saccharide. In some embodiments, the sweet protein includes a combination of any one of the sweet proteins selected from SEQ ID NO: 14- SEQ ID NO:351, SEQ ID NO:353-SEQ ID NO:479 or any combination thereof and the saccharide.

[0188] In some embodiments, the sweet protein includes a combination of any one of the sweet proteins defined in Table 20 shown below and the saccharide.

[0189] In some embodiments, the saccharide can be bound or unbound to the one or more herein-disclosed sweet proteins.

[0190] In some embodiments, the saccharide may be selected from, but not limited to, mannan disaccharide, mannan polysaccharide, mannan oligosaccharide (MOS), betaglucan disaccharide, beta-glucan polysaccharide, beta-glucan-oligosaccharide, alphaglucan disaccharide, alpha-glucan polysaccharide, alpha-glucan-oligosaccharide, chitin disaccharides, chitin polysaccharide, chito oligosaccharide, galactomannan polysaccharide, galactomanno-oligosaccharide, xyloglucan polysaccharide, xyloglucanoligosaccharide, rhamnomannan polysaccharide, rhamnomanno disaccharide, rhamnomanno-oligosaccharide, phosphomannan disaccharide, phosphomannan polysaccharide, phosphomanno-oligosaccharide, galactoglucomannan disaccharide, galactoglucomannan polysaccharide, galactoglucomanno-oligosaccharide, pullulan disaccharide, pullulan polysaccharide, pullulan-oligosaccharide, xylan disaccharide, xylan polysaccharide, xylo-oligosaccharide (XOS), fructan disaccharide, fructan polysaccharide, fructo-oligosaccharide (FOS), laminarin disaccharide, laminarin polysaccharide, laminarin-oligosaccharide, levan polysaccharide, levan disaccharide, levan-oligosaccharide, arabinoxylan disaccharide, arabinoxylan polysaccharide, arabinoxylo-oligosaccharide, glucuronomannan disaccharide, glucuronomannan polysaccharide, glucuronomanno-oligosaccharide, glycosaminoglycan disaccharide, glycosaminoglycan polysaccharide, glycosaminoglycan-oligosaccharide, trehalose, trehalose disaccharide, trehalose-oligosaccharide, peptidoglycan disaccharide, peptidoglycan polysaccharide, peptido-oligosaccharide, and any combination thereof. Each possibility is a separate embodiment.

[0191] In some embodiments, the herein-disclosed saccharide may be selected from, but not limited to, mannan disaccharide, mannan polysaccharide, mannan oligosaccharide (MOS), beta-glucan disaccharide, beta-glucan polysaccharide, beta-glucan oligosaccharides, alpha-glucan disaccharide, alpha-glucan polysaccharide, alpha-glucan- oligosaccharide, chitin disaccharide, chitin polysaccharide, chito oligosaccharide, and any combination thereof. Each possibility is a separate embodiment. According to some embodiments, the polysaccharide includes mannan polysaccharide and / or mannan oligosaccharide (MOS).

[0192] In some embodiments, the herein-disclosed saccharide may include mannose, glucose, galactose, rhamnose, N-acetylglucosamine, N-acetylgalactosamine, N- acetylmuramic acidxylose, fucose, arabinose, trehalose, fructose, maltotriose, glucuronic acid, iduronic acid, hyaluronic acid, N-acetylmuramic acid, 3-deoxy-D-manno-oct-2- ulosonic acid, Chondroitin Sulfate, Dermatan Sulfate, Heparan Sulfate, Keratan Sulfate, glycerol, glycerol phosphate, ribitol, ribitol phosphate, L-glycero-D-manno-heptose, mannuronic acid, or any combination thereof. Each possibility is a separate embodiment.

[0193] According to some embodiments, the herein-disclosed saccharide may be further modified. In some embodiments, the modification of the herein-disclosed saccharide may include, but is not limited to, phosphorylation, methylation, acylation, acetylation, N- acetylation, sulphation, amination, peptide-conjugation, lipid -conjugation, carboxylation, or any combination thereof. Each possibility is a separate embodiment.

[0194] According to some embodiments, the sweet protein is one or more sweet proteins. According to some embodiments, the sweet protein is a combination of 2, 3, 4, 5, or more sweet proteins. Each possibility is a separate embodiment. According to some embodiments, the combination includes 2, 3, 4, 5, or more sweet protein sequences selected from the sequences set forth in SEQ ID NO:1 to SEQ ID NO:479. Each possibility is a separate embodiment. It should be noted that when refereeing to a combination of 2, 3, 4, 5 or more sweet protein, it encompasses a combination of different sweet proteins.

[0195] The following publications are hereby incorporated by reference in their entirety for their teachings and content of the protein: W024003917, WO23181043, WO22137121, WO19215730.

[0196] In some embodiments, the at least one sweet protein is at a concentration of at least about 0.00001%w / w, at least about 0.00005%w / w, at least about 0.00007%w / w, at least about 0.0001%w / w, out of the total weight of the nutraceutical product. In some embodiments, the at least one sweet protein is at a concentration of at least about 0.0001%w / w, atleast about 0.0002%w / w, at least about 0.0003%w / w, at times at least about 0.0005%w / w, at times at least about 0.0007%w / w, at times at least about 0.001%w / w, at times at least about 0.002%w / w, at times at least about 0.003%w / w, at times at least about 0.004%w / w, at times at least about 0.005%w / w, at times at least about 0.006%w / w, at times at least about 0.007%w / w, at times at least about 0.008%w / w, at times at least about 0.009%w / w, at times at least about 0.010%w / w, at times at least about 0.011%w / w, at times at least about 0.012%w / w, at times at least about 0.013%w / w, at times at least about 0.014%w / w, at times at least about 0.015%w / w, at times at least about 0.016%w / w, at times at least about 0.017%w / w, at times at least about 0.018%w / w, at times at least about 0.019%w / w, at times at least about 0.020%w / w, at times at least about 0.030%w / w out of the total weight of the nutraceutical product.

[0197] In some embodiments, the at least one sweet protein is at a concentration of at most about l%w / w, at times at most about 0.7%w / w, at times at most about 0.5%w / w, at times at most about 0.3%w / w, at times at most about 0.2%w / w, at times at most about 0.15%w / w, at times at most about 0.1%w / w, at times at most about 0.08%w / w, at times at most about 0.07%w / w, at times at most about 0.05%w / w, at times at most about 0.03%w / w, at times at most about 0.025%w / w, at times at most about 0.024%w / w, at times at most about 0.023%w / w, at times at most about 0.022%w / w, at times at most about 0.021%w / w, at times at most about 0.020%w / w, at times at most about 0.019%w / w, at times at most about 0.018%w / w, at times at most about 0.017%w / w, at times at most about 0.016%w / w, at times at most about 0.015%w / w, at times at most about 0.014%w / w, at times at most about 0.013%w / w, at times at most about 0.012%w / w, at times at most about 0.01 l%w / w, at times at most about 0.01%w / w, at times at most about 0.009%w / w, at times at most about 0.008%w / w, at times at most about 0.007%w / w, at times at most about 0.006%w / w, at times at most about 0.005%w / w, at times at most about 0.004%w / w, at times at most about 0.003%w / w, out of the total weight of the nutraceutical product.

[0198] In some embodiments, the at least one sweet protein is at a concentration of about 0.00001%w / w, at times of about 0.00003%w / w, at times of about 0.00005%w / w, at times of about 0.00007%w / w out of the total weight of the nutraceutical product.

[0199] In some embodiments, the at least one sweet protein is at a concentration of about 0.0001% w / w, at times about 0.0002%w / w, at times about 0.0003%w / w, at times about 0.0005%w / w, at times about 0.0007%w / w, at times about 0.001%w / w, at times about 0.002%w / w, at times about 0.003%w / w, at times about 0.004%w / w, at times about

[0200] 0.005%w / w, at times about 0.006%w / w, at times about 0.007%w / w, at times about

[0201] 0.008%w / w, at times about 0.009%w / w, at times about 0.010%w / w, at times about

[0202] 0.011%w / w, at times about 0.012%w / w, at times about 0.013%w / w, at times about

[0203] 0.014%w / w, at times about 0.015%w / w, at times about 0.016%w / w, at times about

[0204] 0.017%w / w, at times about 0.018%w / w, at times about 0.019%w / w, at times about

[0205] 0.020%w / w, at times about 0.030%w / w out of the total weight of the nutraceutical product.

[0206] In some embodiments, the at least one sweet protein is at a concentration of about 0.001%w / w, at times about 0.002%w / w, at times about 0.003%w / w, at times about 0.004%w / w, at times about 0.008%w / w, out of the total weight of the nutraceutical product.

[0207] In some embodiments, the at least one sweet protein is at a concentration of between about 0.00001%w / w and about l%w / w, at times between about 0.00003%w / w and about l%w / w out of the total weight of the nutraceutical product.

[0208] In some embodiments, the at least one sweet protein is at a concentration of between about 0.0001%w / w and about l%w / w, at times between about 0.0003%w / w and about l%w / w, at times between about 0.0005%w / w and about 0.7%w / w, at times between about 0.0005%w / w and about 0.5%w / w, at times between about 0.0005%w / w and about 0.3%w / w, at times between about 0.0005%w / w and about 0.1%w / w, at times between about 0.0005%w / w and about 0.07%w / w, at times between about 0.0005%w / w and about 0.05%w / w, at times between about 0.0005%w / w and about 0.03%w / w, at times between about 0.0007%w / w and about 0.03%w / w, at times between about 0.001%w / w and about 0.02%w / w out of the total weight of the nutraceutical product.

[0209] In some embodiments, the at least one sweet protein is at a concentration of between about 0.0001%w / w and about 0.0007% w / w out of the total weight of the nutraceutical product.

[0210] In some embodiments, the at least one sweet protein is at a concentration of between about 0.003%w / w and about 0.007%w / w, out of the total weight of the nutraceutical product. In some embodiments, the at least one sweet protein is at a concentration of between about 0.005%w / w and about 0.035%w / w out of the total weight of the nutraceutical product.

[0211] In some embodiments, the at least one sweet protein is at a concentration of between about 0.001%w / w and about 0.02%w / w, at times between about 0.001%w / w and about 0.015%w / w, at times between about 0.001%w / w and about 0.01%w / w out of the total weight of the nutraceutical product.

[0212] In some embodiments, the at least one sweet protein is at a concentration of between about 0.01%w / w and about 0.15%w / w, at times between about 0.01%w / w and about 0.1%w / w out of the total weight of the nutraceutical product.

[0213] In some embodiments, the at least one sweet protein is at a concentration of between about 0.01%w / w and about 0.07%w / w, at times between about 0.01%w / w and about 0.05%w / w, at times between about 0.01%w / w and about 0.03%w / w, out of the total weight of the nutraceutical product.

[0214] The nutraceutical product may comprise additional sweeteners aside of the at least one sweet protein.

[0215] In some examples, the nutraceutical product comprises stevia or any derivative thereof. In some examples, the stevia is or comprises one or more of steviol glycoside, Rebaudioside A (Reb A), Rebaudioside M (Reb M), Rebaudioside D (Reb D), Stevioside, Rebaudioside or a combination thereof.

[0216] In some examples, the nutraceutical product comprises one or more sweetener selected from the group consisting of at least one sugar, non-nutritive sweetener, high intensity sweetener, a natural sweetener, a sugar alcohol, a rare sugar, an amino acid or a combination thereof.

[0217] In some embodiments, the sugar is one or more of sucrose, glucose, lactose, fructose, palatinose, sucralose, com syrup, high fructose com syrup, invert sugar, dextrose, powder sugar, or a combination thereof, in any form of liquid, powder, or solid.

[0218] In some embodiments, the high intensity sweetener is one or more of saccharin, aspartame, acesulfame, potassium (ace-k), neotame, advantame or a combination thereof.

[0219] In some embodiments, the amino acid is glycine. In some embodiments, the natural sweetener is one or more of stevia, sweet truffle protein (mycodulcein), brazzein, monk fruit, honey, agave, maple syrup, silan, black treacle, molasses, glycine or a combination thereof.

[0220] In some embodiments, the sugar alcohol is one or more of mannitol, maltitol, sorbitol, xylitol, lactitol, isomalt, erythritol or a combination thereof.

[0221] In some embodiments, the rare sugar is one or more of allulose, turanose, isomaltulose, isomaltose, tagarose or a combination thereof.

[0222] According to some embodiments, the non-nutritive sweetener is selected from a group consisting of aspartame, sucralose, saccharin, stevia, acesulfame potassium, neotame, allulose, monk fruit extract, erythritol, xylitol, mannitol, sorbitol, cyclamate, and any combination thereof.

[0223] The product described herein may comprise additional components. In some examples, the food product may comprise one or more proteins in addition to the sweet proteins described herein. As appreciated, by incorporating additional proteins, the product aims to provide a higher nutritional profile. In accordance with the present disclosure, the one or more proteins may be considered as a bioactive compound.

[0224] In some examples, the food product comprises one or more non-taste-modulating proteins. The term non-taste-modulating protein denoted a protein that does not contribute to sweetness of the product.

[0225] In some examples, the food product comprises one or more of a whey protein, a casein protein, a soy protein, a pea protein, a rice protein, a chickpea protein or any combination thereof.

[0226] In some examples, the food product comprises one or more of a whey protein, a casein protein, an egg protein, a soy protein, a pea protein, a rice protein, a chickpea protein, a hemp protein, a fava bean protein, a lentil protein, an oat protein, a quinoa protein, a mycoprotein, an algal protein, a collagen protein, or any combination thereof.

[0227] The present disclosure is not limited to a specific food product and as noted herein, may comprise a dietary supplement or a functional food.

[0228] In some embodiments, the composition (also referred to herein as “food product”) comprises 20%-500%, 30%-200%, 50%-100% or about 75%-150% or any other range within the range of 20%-500% of the daily recommended value (dv). Each possibility is a separate embodiment.

[0229] In some embodiments, the ratio between each of the at least one bioactive and the protein sweetener is at least about 0.5%, at least about 1%, at least about 10%, at least about 15%, at least about 20%, at least about 25%, at least at least about 50%, at least about 75%. Each possibility is a separate embodiment, some embodiments, the ratio between each of the at least one bioactive and the protein sweetener is between 0.5% and 500%, or 0.5% and 300%, between 1% and 100% or any other range between 0.5% and 1000%. Each possibility is a separate embodiment.

[0230] In some embodiments, the composition comprises at least two, at least three or at least four different bioactive compounds. Each possibility is a separate embodiment.

[0231] In some embodiments, the composition comprises at least two, at least three or at least four different sweet proteins. Each possibility is a separate embodiment.

[0232] According to some embodiments, the composition is a gummy candy. According to some embodiments, the composition comprises water, maltodextrin, at least one bioactive and at least one protein sweetener. According to some embodiments, the composition further comprises fibers (such as but not limited to inulin). According to some embodiments, the composition further comprises a syrup, such as but not limited to com syrup, FOS syrup, GOS syrup, mannitol syrup or any combination thereof. Each possibility is a separate embodiment. According to some embodiments, the composition further comprises sucrose and / or fructose and / or dextrose. According to some embodiments, the composition further comprises pectin. According to some embodiments the composition further comprises a high intensity sweetener, such as but not limited to stevia or a stevia derivative.

[0233] According to some embodiments, the composition is a sugar reduced chewable bioactive composition. According to some embodiments, the composition comprises water, maltodextrin, at least one bioactive (e.g. vitamin) and at least one protein sweetener. According to some embodiments, the composition further comprises a gum base.

[0234] According to some embodiments, the composition is a sugar reduced energy drink (carbonated or non-carbonated). According to some embodiments, the composition comprises less than 15%, less than 12%, less than 10% or less than 8% sugar / sucrose, at least one bioactive and at least one sweet protein. Each possibility is a separate embodiment.

[0235] According to some embodiments, the composition is a sugar reduced energy / snack bar / protein bar. According to some embodiments, the composition comprises oatmeal, at least one bioactive and at least one sweet protein.

[0236] According to some embodiments, the composition is a sugar reduced milk / milk alternative drink. According to some embodiments, the composition comprises milk (or milk alternative such as but not limited to soy milk, almond milk, oatmeal milk or the like), water, whey powder, at least one bioactive and at least one sweet protein.

[0237] According to some embodiments, the composition is a sugar reduced electrolyte powder. According to some embodiments, the electrolyte powder sucrose and / or fructose, maltodextrin, at least one bioactive and one or more sweet proteins. According to some embodiments, the composition is a lozenge, troche or pastille. According to some embodiments, the lozenge comprises Microcrystalline Cellulose, dextrose, at least one bioactive and at least one sweet protein.

[0238] The food product being a functional food product or a dietary supplement food product is in accordance with some embodiments, a reduced sugar product.

[0239] The food product, being a functional food product or a dietary supplement food product, is, in accordance with some embodiments, a reduced sugar product. As used herein, a reduced sugar product refers to a functional food product or dietary supplement that contains a lower amount of sugar compared to its conventional full-sugar counterpart, achieved through partial sugar replacement, sugar reduction, or reformulation with alternative sweeteners, while maintaining sensory and functional properties. In some embodiments, the reduced sugar product is formulated with at least one sweet protein, in comparison to the same functional food product or dietary supplement that is essentially free of sweet protein.

[0240] In accordance with the present disclosure, when referring to the conventional counterpart, it should be understood as the conventional counterpart being a full sugar or a reduced sugar product that is essentially free of a sweet protein. In some examples, the conventional counterpart is a full sugar product. In some examples, the conventional counterpart is a reduced sugar product.

[0241] In some examples, in which the conventional counterpart is a reduced sugar counterpart, the one or more sweet proteins may replace (substitute) the one or more sugar-alternative sweeteners (non-sugar). The replacement may be a partial replacement or a full replacement.

[0242] In some examples, in which the conventional counterpart is a reduced sugar counterpart, the one or more sweet proteins may be added to the one or more sugaralternative sweeteners (non-sugar).

[0243] In some examples, the amount of sugar may not be affected by the partial or full replacement of the non-sugar sweeteners. In some examples, the amount of sugar may not be affected by the partial or full replacement of the non-sugar sweeteners thereby a similar % of sugar may be maintained.

[0244] In some examples, the amount of sugar may be reduced by the partial or full replacement of the non-sugar sweeteners as compared to the same product that is essentially free of a sweet protein.

[0245] In accordance with some embodiments, in the reduced sugar product, the sugar content is reduced by at least 25% as compared to the same product that is essentially free of a sweet protein.

[0246] In some examples, the food product is characterized by a similar sweetness level as compared to the same food product that is essentially free of a sweet protein. It should be noted that "a similar sweetness level" encompasses a minimal change, up to about 5%, up to about 10%, up to about 15%, up to about 20% change.

[0247] The term "sweetness level" refers to a quantitative and / or qualitative measurement relative to a reference sweetener. The sweetness level may be determined by any method known in the art. For example, the sweetness level may be determined by a sensory analysis method. In some examples, the sweetness level may be determined by a sensory panel as described in the Examples below.

[0248] In some examples, the food product is one or more of a gummy candy, chewable product, an edible film strip, a hard candy, a powder, a liquid, a powder, a bar, a tablet or any combination thereof. As shown in Example 1 below, the food product is gummy candy. In some examples, the food product is gummy candy. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound.

[0249] In some examples, the food product being a gummy candy comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a gummy candy essentially free of a sweet protein.

[0250] In some examples, the food product being a gummy candy comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a gummy candy essentially free of a sweet protein.

[0251] In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to a gummy candy essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the gummy candy essentially free of a sweet protein may be a reduced sugar gummy candy.

[0252] In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO :350) or a variant thereof. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 16 (denoted herein as DM08), SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO: 53 (denoted herein as DM69), SEQ ID NO: 104 (denoted herein as DM137), SEQ ID NO: 186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO: 8) or a variant thereof. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof.

[0253] In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is my codulcein (denoted herein as SEQ ID NO:352). or a variant thereof. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, mycodulcein or a variant thereof or any combination thereof.

[0254] In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Thaumatin 1 (denoted herein as SEQ ID NO:3) Thaumatin 2 (denoted herein as SEQ ID NON) or a variant thereof. In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Thaumatin or a variant thereof or any combination thereof.

[0255] In some examples, the food product being a gummy candy comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a gummy candy comprises one or more sweet proteins as described in any one of Table lA-Table 1 J or any combination thereof. In some examples, the food product being a gummy candy comprises one or more bioactive compounds as described in any one of Table 1 A-Table 1J or any combination thereof. In some examples, the food product being a gummy candy is as shown in Table 1 A. In some examples, the food product being a gummy candy is as shown in Table IB . In some examples, the food product being a gummy candy is as shown in Table 1C. In some examples, the food product being a gummy candy is as shown in Table ID. In some examples, the food product being agummy candyis as shown in Table IE. In some examples, the food product being a gummy candy is as shown in Table IF. In some examples, the food product being a gummy candy is as shown in Table 1G. In some examples, the food product being agummy candyis as shown in Table 1H. In some examples, the food product being a gummy candy is as shown in Table II. In some examples, the food product being a gummy candy is as shown in Table 1 J. In some examples, the food product is a chewable product. In some examples, the chewable product is a one or more of a chewable vitamin, a chewable candy, a chewing gum or a combination thereof.

[0256] As shown in Example 2 below, the food product is a chewable vitamin. In some examples, the food product is a chewable vitamin. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound.

[0257] In some examples, the food product being a chewable vitamin comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a chewable vitamin essentially free of a sweet protein.

[0258] In some examples, the food product being a chewable vitamin comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a chewable vitamin essentially free of a sweet protein.

[0259] In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to a chewable vitamin essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the chewable vitamin essentially free of a sweet protein may be a reduced sugar chewable vitamin.

[0260] In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an an amino acid sequence denoted by one or more of SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:351 (denoted herein as DM170), SEQ ID NO: 16 (denoted herein as DM08), a protein denoted by SEQ ID NO:257 (denoted herein as DM532) or any combination thereof. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8) or a variant thereof. In some examples, thefood productbeing a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is My codulcein (denoted herein as SEQ ID NO :352) or a variant thereof. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, My codulcein or a variant thereof or any combination thereof. In some examples, the food product being a chewable vitamin comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a chewable vitamin is as shown in Table 2A.

[0261] In some examples, the food product is a liquid. In some examples, the liquid is a sports drink, an energy drink, a carbonated energy drink, a sport nutrition drink, a protein milk drink, a functional beverage, a drink shot, a shake, a hydration, a liquid gel or a combination thereof

[0262] As shown in Example 3 below, the food product is energy drink. In some examples, the food product is energy drink. In some examples, the food product is a noncarbonated energy drink. In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound.

[0263] In some examples, the food product being an energy drink comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to an energy drink essentially free of a sweet protein.

[0264] In some examples, the food product being an energy drink comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to an energy drink essentially free of a sweet protein.

[0265] In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to energy drink essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the energy drink essentially free of a sweet protein may be a reduced sugar energy drink.

[0266] In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO:14 or SEQ ID NO:350) or a variant thereof. In some examples, the food productbeing energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 16 (denoted herein as DM08), SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:104 (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8). or a variant thereof. In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one stimulant.

[0267] In some examples, the food product being an energy drink comprises one or more bioactive compounds as described in any one of Table 3 A- Table 3E. In some examples, the food product being an energy drink comprises one or more sweet proteins as described in any one of Table 3A- Table 3E. In some examples, the food product being energy drink is as shown in Table 3 A. In some examples, the food product being energy drink is as shown in Table 3B. In some examples, the food product being energy drink is as shown in Table 3C. In some examples, the food product being energy drink is as shown in Table 3D. In some examples, the food product being energy drink is as shown in Table 3E.

[0268] As shown in Example 4 below, the food product is carbonated energy drink. In some examples, the food product is carbonated energy drink. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one stimulant.

[0269] In some examples, the food product being a carbonated energy drink comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a carbonated energy drink essentially free of a sweet protein.

[0270] In some examples, the food product being a carbonated energy drink comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a carbonated energy drink essentially free of a sweet protein.

[0271] In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to carbonated energy drink essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the carbonated energy drink essentially free of a sweet protein may be a reduced sugar carbonated energy drink.

[0272] In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 16 (denoted herein as DM08), SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NOTCH (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170)or any combination thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO: 8). or a variant thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is mycodulcein (denoted herein as SEQ ID NO:352). or a variant thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being carbonated energy drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a carbonated energy drink comprises one or more bioactive compounds as described in any one of Tables 4A, 4C, 4E, 4G or any combination thereof. In some examples, the food product being a carbonated energy drink comprises one or more sweet proteins as described in any one of Tables 4A, 4C, 4E, 4G or any combination thereof. In some examples, the food product being carbonated energy drink is as shown in Table 4A. In some examples, the food product being carbonated energy drink is as shown in Table 4C. In some examples, the food product being carbonated energy drink is as shown in Table 4E. In some examples, the food product being carbonated energy drink is as shown in Table 4G.

[0273] In some examples, the food product is a bar. In some examples, the bar is a protein bar, an energy bar, a snack bar, a functional bar or any combination thereof.

[0274] As shown in Example 5 below, the food product is protein bar and / or an energy bar. In some examples, the food product is protein bar. In some examples, the food product is snack bar. In some examples, the food product is functional bar. In some examples, the food product being protein bar and / or an energy bar and / or a snack bar and / or a functional bar comprises at least one sweet protein and at least one bioactive compound.

[0275] In some examples, the food product being a protein bar and / or an energy bar and / or a snack bar and / or a functional bar comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a protein bar and / or an energy bar essentially free of a sweet protein.

[0276] In some examples, the food product being a protein bar and / or an energy bar and / or a snack bar and / or a functional bar comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a protein bar and / or an energy bar essentially free of a sweet protein.

[0277] In some examples, the food product being protein bar and / or an energy bar and / or a snack bar and / or a functional bar comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to a bar essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the protein bar and / or an energy bar and / or a snack bar and / or a functional bar essentially free of a sweet protein may be a reduced sugar bar.

[0278] In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 16 (denoted herein as DM08), SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO: 53 (d enoted herein as DM69), SEQ ID NO : 104 (d enoted herein as DM 137), SEQ ID NO : 186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID N0:8) or a variant thereof. In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is My codulcein (denoted herein as SEQ ID NO :352) or a variant thereof. In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being protein bar / energy / snack / functional comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being protein / energy / snack / functional bar comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a protein / energy / snack / functional comprises one or more bioactive compounds as described in any one of Table 5A-Table 5H or any combination thereof. In some examples, the food product being a protein / energy / snack / functional bar comprises one or more sweet proteins as described in any one of Table 5A-Table 5H or any combination thereof. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5A. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5B. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5C. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5D. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5E. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5F. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5G. In some examples, the food product being protein / energy / snack / functional bar is as shown in Table 5H.

[0279] In some examples, the food product is a protein milk drink. As shown in Example 6 below, the food product is a protein milk drink. In some examples, the food product is a protein milk drink. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound.

[0280] In some examples, the food product being a protein milk drink comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a protein milk drink essentially free of a sweet protein.

[0281] In some examples, the food product being a protein milk drink comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a protein milk drink essentially free of a sweet protein.

[0282] In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to a protein milk drink essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the protein milk drink essentially free of a sweet protein may be a reduced sugar protein milk drink.

[0283] In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO:16 (denoted herein as DM08), SEQ ID NO:17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:104 (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8) or a variant thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Mycodulcein (denoted herein as SEQ ID NO:352) or a variant thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being a protein milk drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a protein milk drink comprises one or more sweet proteins as described in any one of Table 6A, Table 6B, Table 6D, Table 6E, Table 6F, Table 6H, Table 61, Table 6J, Table 6L or any combination thereof. In some examples, the food product being a protein milk drink comprises one or more bioactive compounds as described in any one of Table 6A, Table 6B, Table 6D, Table 6E, Table 6F, Table 6H, Table 61, Table 6J, Table 6L or any combination thereof. In some examples, the food product being a protein milk drink is as shown in Table 6A. In some examples, the food product being a protein milk drink is as shown in Table 6B. In some examples, the food product being a protein milk drink is as shown in Table 6D. In some examples, the food product being a protein milk drink is as shown in Table 6E. In some examples, the food product being a protein milk drink is as shown in Table 6F. In some examples, the food product being a protein milk drink is as shown in Table 6H. In some examples, the food product being a protein milk drink is as shown in Table 61. In some examples, the food product being a protein milk drink is as shown in Table 6J. In some examples, the food product being a protein milk drink is as shown in Table 6L.

[0284] As shown in Example 7 below, the food product is a sport nutrition drink. In some examples, the food product is a sport nutrition drink. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound. In some examples, the food product being a sport nutrition drink comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to a sport nutrition drink essentially free of a sweet protein.

[0285] In some examples, the food product being a sport nutrition drink comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to a sport nutrition drink essentially free of a sweet protein.

[0286] In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to a sport nutrition drink essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the sport nutrition drink essentially free of a sweet protein may be a reduced sugar a sport nutrition drink.

[0287] In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO:16 (denoted herein as DM08), SEQ ID NO:17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:104 (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8) or a variant thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is My codulcein (denoted herein as SEQ ID NO:352) or a variant thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin. In some examples, the food product being a sport nutrition drink comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one non-sweet protein.

[0288] In some examples, the food product being a sport nutrition drink comprises one or more bioactive compounds as described in any one of Table 7A, Table 7B, Table 7D or any combination thereof. In some examples, the food product being a sport nutrition drink comprises one or more sweet proteins as described in any one of Table 7A, Table 7B, Table 7D or any combination thereof. In some examples, the food product being a sport nutrition drink is as shown in Table 7A. In some examples, the food product being a sport nutrition drink is as shown in Table 7B. In some examples, the food product being a sport nutrition drink is as shown in Table 7D.

[0289] In some examples, the food product is an edible film strip being an oral thin film strip or edible film.

[0290] As shown in Example 8 below, the food product is an oral film strip. In some examples, the food product is an oral film strip. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound.

[0291] In some examples, the food product being an oral film strip comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to an oral film strip essentially free of a sweet protein.

[0292] In some examples, the food product being an oral film strip comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared to an oral film strip essentially free of a sweet protein. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to an oral film strip essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the oral film strip essentially free of a sweet protein may be a reduced sugar oral film strip.

[0293] In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO:14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO: 16 (denoted herein as DM08), SEQ ID NO: 17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:104 (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8). or a variant thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is My codulcein (denoted herein as SEQ ID NO:352). or a variant thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being an oral film strip comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin.

[0294] In some examples, the food product being an oral film strip comprises one or more bioactive compounds as described in any one of Table 8A-Table 8C or any combination thereof. In some examples, the food product being an oral film strip comprises one or more sweet proteins as described in any one of Table 8A-Table 8C or any combination thereof. In some examples, the food product being an oral film strip is as shown in Table 8 A. In some examples, the food product being an oral film strip is as shown in Table 8B. In some examples, the food product being an oral film strip is as shown in Table 8C.

[0295] In some examples, the food product is a powder for reconstituting as a drink, a meal, a shake, a capsule.

[0296] In some examples, the food product is a powder being an electrolyte powder.

[0297] As shown in Example 9 below, the food product is an electrolyte powder. In some examples, the food product is an electrolyte powder. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound .

[0298] In some examples, the food product being an electrolyte powder comprising at least one sweet protein and at least one bioactive compound is characterized by the same sweetness level as compared to an electrolyte powder essentially free of a sweet protein.

[0299] In some examples, the food product being an electrolyte powder comprising at least one sweet protein and at least one bioactive compound is characterized by a lower sweetness level as compared an electrolyte powder essentially free of a sweet protein.

[0300] In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound is characterized by at least about 30% sugar reduction as compared to an electrolyte powder essentially free of a sweet protein, at times at least 40% sugar reduction, at times at least about 50% sugar reduction, at times at least about 60% sugar reduction, at times at least about 70% sugar reduction, at times at least about 80% sugar reduction, at times at least about 90% sugar reduction. As noted herein, the electrolyte powder essentially free of a sweet protein may be a reduced sugar electrolyte powder. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is a single chain Monellin (MNEI) protein (SEQ ID NO: 14 or SEQ ID NO:350) or a variant thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein has an amino acid sequence denoted by one or more of SEQ ID NO:16 (denoted herein as DM08), SEQ ID NO:17 (denoted herein as DM09), SEQ ID NO:39 (denoted herein as DM31), SEQ ID NO:53 (denoted herein as DM69), SEQ ID NO:104 (denoted herein as DM137), SEQ ID NO:186 (denoted herein as DM165), SEQ ID NO:217 (denoted herein as DM685), SEQ ID NO:257 (denoted herein as DM532), SEQ ID NO:263 (denoted herein as DM528), SEQ ID NO:351 (denoted herein as DM170) or any combination thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Brazzein (denoted herein as SEQ ID NO:8). or a variant thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Brazzein or a variant thereof or any combination thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is Mycodulcein (denoted herein as SEQ ID NO:352). or a variant thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one sweet protein is MNEI or a variant thereof, Mycodulcein or a variant thereof or any combination thereof. In some examples, the food product being an electrolyte powder comprises at least one sweet protein and at least one bioactive compound, wherein the at least one bioactive compound is at least one vitamin.

[0301] In some examples, the food product being as electrolyte powder comprises one or more bioactive compounds as described in any one of Table 9A and / or Table 9C. In some examples, the food product being as electrolyte powder comprises one or more sweet proteins as described in any one of Table 9A and / or Table 9C. In some examples, the food product being an electrolyte powder is as shown in Table 9A. In some examples, the food product being an electrolyte powder is as shown in Table 9C. In some examples, the food product is a hard candy being pop rocks.

[0302] According to some embodiments, the food product comprises one or more of water, baking soda, wheat flour, binding agent, and / or a salt.

[0303] In some embodiments, the food product comprises at least one salt.

[0304] The term salt in the context of the present disclosure refers to an ionic compound composed of cations (positively charged ions) and anions (negatively charged ions) that are typically formed by the neutralization reaction of an acid and a base.

[0305] In some examples, the at least one salt is a mineral salt. In some examples, the at least one salt is an organic salt.

[0306] According to some embodiments, the salt may include salt having a cation selected from Na+, K+, Ca2+and Mg2+and an anion.

[0307] In some examples, the salt is a sodium salt. In some examples, the salt is a potassium salt. In some examples, the salt is a calcium salt. In some examples, the salt is a magnesium salt. In some examples, the salt is a phosphorous salt. The anion, in some embodiments, may be selected from lactate, chloride, citrate, gluconate, benzoate, lactate gluconate, anhydrous and / or hydrate forms thereof, derivative thereof, and any combinations thereof. In some embodiments, the anion can be selected from gluconate, citrate, hydrogen citrate, dihydrogen citrate, malate, hydrogen malate, maleate, hydrogen maleate, fumarate, hydrogen fumarate, succinate, hydrogen succinate, glutarate, hydrogen glutarate, adipate, hydrogen adipate, lactate, tartrate, bitartrate, phosphate, monohydrogen phosphate, dihydrogen phosphate, fluoride, sulfate, bisulfate, nitrate, carbonate, bicarbonate, glycerate, glycolate, derivative thereof, or a combination thereof. In some embodiments, the salt is selected from magnesium lactate, magnesium citrate, calcium citrate, calcium lactate, calcium gluconate, calcium lactate gluconate, an anhydrous and / or hydrate form thereof, and a combination thereof. Each possibility is a separate embodiment.

[0308] According to some other embodiments, the salt may be selected from sodium chloride, potassium chloride, magnesium sulfate, calcium chloride, sodium bicarbonate, sodium citrate, calcium lactate, sodium acetate, potassium citrate, sodium phosphate, calcium carbonate, magnesium chloride, potassium bicarbonate, zinc sulfate, calcium phosphate, ammonium chloride, sodium lactate, potassium carbonate, sodium ascorbate, calcium ascorbate, derivative thereof, and any combination thereof.

[0309] In some examples, the at least one salt is one or more of sodium chloride, sodium bicarbonate, sodium citrate or any combination thereof.

[0310] In some examples, the at least one salt is one or more of potassium chloride, potassium sorbate, potassium citrate or any combination thereof.

[0311] In some examples, the at least one salt is one or more of calcium carbonate, calcium chloride, calcium propionate or any combination thereof.

[0312] In some examples, the at least one salt is magnesium sulfate and / or magnesium carbonate.

[0313] In some examples, the at least one salt is one or more of monosodium phosphate, disodium phosphate, sodium tripolyphosphate or any combination thereof.

[0314] In some examples, the at least one salt is one or more of sodium lactate, calcium lactate, potassium acetate or any combination thereof.

[0315] In some embodiments, the nutraceutical product comprises zinc oxide.

[0316] In some embodiments, the nutraceutical product further includes a GRAS compound selected from, but not limited to, GRAS compound 4701, GRAS compound 4965, GRAS compound 4669, or a combination thereof.

[0317] The term "about" as used herein indicates values that may deviate up to 1%, more specifically 5%, more specifically 10%, more specifically 15%, and in some cases up to 20% higher or lower than the value referred to, the deviation range including integer values, and, if applicable, non-integer values as well, constituting a continuous range. In some embodiments, the term "about" refers to ± 10%. In some embodiments, the term "about" refers to ± 20%.

[0318] As used herein, the forms "a", "an" and "the" include singular as well as plural references unless the context clearly dictates otherwise.

[0319] It is appreciated that certain features of the invention, which are, for clarity, described in the context of separate embodiments, may also be provided in combination in a single embodiment. Conversely, various features of the invention, which are, for brevity, described in the context of a single embodiment, may also be provided separately or in any suitable sub combination or as suitable in any other described embodiment of the invention. Certain features described in the context of various embodiments are not to be considered essential features of those embodiments unless the embodiment is inoperative without those elements.

[0320] It should be noted that the various embodiments and examples detailed herein in connection with various aspects of the invention may be applicable to one or more aspects disclosed herein. It should be further noted that any embodiment described herein may be applied separately or in various combinations. Various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below find experimental support in the following examples. The phrases “in another embodiment” or any refence made to embodiment as used herein do not necessarily refer to different embodiment, although it may. Thus, various embodiments of the invention can be combined (from the same or from different aspects) without departing from the scope of the invention.

[0321] Various embodiments and aspects of the present invention as delineated herein above and as claimed in the claims section below find experimental support in the following examples.

[0322] Disclosed and described, it is to be understood that this invention is not limited to the particular examples, products disclosed herein as such products, may vary somewhat. It is also to be understood that the terminology used herein is used for the purpose of describing particular embodiments only and not intended to be limiting since the scope of the present invention will be limited only by the appended claims and equivalents thereof.

[0323] The following examples are provided to illustrate specific embodiments of the invention and are described with reference to particular sweet proteins. However, it should be understood that these examples are not limiting, and the disclosed products and methods are broadly applicable to any sweet protein or combination thereof. The term "sweet protein" is as defined herein and encompasses all proteins that elicit a sweet taste, including naturally occurring proteins, engineered variants, and synthetic analogs. The use of one or more sweet proteins in these examples is merely illustrative, and results may be achieved with other sweet proteins. The following examples are representative of techniques employed by the inventors in carrying out aspects of the present invention. It should be appreciated that while these techniques are exemplary of preferred embodiments for the practice of the invention, those of skill in the art, in light of the present disclosure, will recognize that numerous modifications can be made without departing from the spirit and intended scope of the invention.

[0324] Table 20: List of sweet proteins NON-LIMITING EXAMPLES

[0325] Sensory Evaluation - Expert Panel

[0326] Unless indicated otherwise, sensory evaluations were determined using a panel of tasters for analytical discrimination.

[0327] The sensory panel was established by a screening process of potential tasters. Screening tests, conducted according to ISO standard (IS 8586-1), examined the sensory sensitivity, consistency, and sensory memory of the taster. The panel is well trained and calibrated. The selected panel is trained regularly to maintain high performance output.

[0328] Sensory profiles - testing procedure:

[0329] Sensory vocabulary was determined by the expert panel. The sensory vocabulary was built by tasting a range of products from the category and raising all the relevant sensory attributes which describe the category. Using as diverse a language as possible to best describe the products.

[0330] After having the sensory vocabulary, selecting the key attributes that will be used to describe the products in the questionnaire.

[0331] Building sensory profiles: the panelists rated each tested product versus a reference on a two-way scale (between -3 to +3) with a fixed reference point (0) across all the attributes selected from the glossary. When the tested product was evaluated “more” than the reference on a specific attribute (e.g., sweeter, thicker, and so on), it got positive rates (+1, +2, or +3), and when it was rated “less” than the reference on a specific attribute (e.g., less sweet, less thick, and so on) it got negative rates (-1, -2 or -3). Before and between attributes, the tasters were requested to rinse their mouths with mineral water, eat an unsalted cracker and cucumber, and drink water again.

[0332] Example 1: Gummy candy with sweet proteins and bioactive ingredients

[0333] Example 1A: Preparation and characterization of gummy candy comprising DM31

[0334] Methods

[0335] Maltodextrin, fibers, sucrose, pectin, and modified starch were mixed together, then water was added to the mixture. The aqueous mixture was boiled to 100°C. Then, com syrup was added while stirring and the mixture was heated to 105°C. When reaching this temperature, the mass was cooled down to 60°C. At 60°C sweet protein DM31, food coloring and flavor were added while mixing well, resulting in a mass of gummy candy.

[0336] The list of ingredients and their amounts is shown in Table 1A.

[0337] Table 1A: list of ingredients

[0338] Results

[0339] Figure 1 shows a spider plot of gummy candy with 50% sugar reduction compared to gummy candy with 50% sugar reduction with sweet protein DM-31.

[0340] As can be seen from Figure 1, gummy candy with 50% sugar reduction combined with DM31 is sweeter than gummy candy with 50% sugar reduction without added protein.

[0341] In addition, and as can be seen from Figure 1, properties such as sourness and off-flavor which at times are considered unfavorable properties are similar with and without addition of DM31. This implies that replacement of sugar by sweet protein such as DM31 is highly favorable. Example IB: Sugar-reduced gummy candy comprising DM31

[0342] Methods

[0343] The ingredients listed in Table IB, except sweet protein DM31, water and com syrup are mixed well, then water is added, and the mixture is heated to boiling at 100°C. Afterhearing, com syrup is added while stirring and cooked to 105°C. The mass is cooled to 60°C and sweet protein DM-31, food coloring and flavor are added.

[0344] The list of ingredients and their amounts is shown in Table IB.

[0345] Table IB: list of ingredients Example 1C: Gummy candy comprising DM685

[0346] Methods

[0347] Maltodextrin, fibers, sucrose, pectin, and modified starch are mixed together, then water is added to the mixture. The aqueous mixture is boiled to 100°C. Then, com syrup is added while stirring and the mixture is heated to 105°C. When reaching this temperature, the mass is cooled down to 60°C. At 60°C sweet protein DM685, food coloring and flavor are added while mixing well, resulting in a mass of gummy candy.

[0348] The list of ingredients and their amounts is shown in Table 1C.

[0349] Table 1C: list of ingredients

[0350] Example ID: Gummy candy comprising DM31 with 50% sugar reduction

[0351] Methods

[0352] About 50% of the water is heated to 50°C. 50% of the citric acid, all tri sodium, pectin, and half of the FOS and GOS syrups are mixed. The mixture is added to the warm water and heated to 90°C. Sucrose and 50% of FOS syrup are added and the mixture is heated to 110°C and mixed well. Additional water is added and mixed well and then flavor and color additive are added. Then, 50% of GOS syrup, vitamins, sweet protein, and 50% of citric acid are added while stirring. The mixture is poured into pre-oiled molds and allowed to set.

[0353] The list of ingredients and their amounts is shown in Table ID

[0354] Table ID: list of ingredients

[0355] Example IE: Gummy candy with combination of sweet proteins and 50% sugar reduction

[0356] Methods:

[0357] About 50% of the water is heated to 50°C. 50% of the citric acid, all tri sodium, pectin, and half of the FOS and GOS syrups are mixed. The mixture is added to the warm water and heated to 90°C. Sucrose and 50% of FOS syrup are added and the mixture is heated to 110°C and mixed well. Additional water is added and mixed well and then flavor and color additive are added. Then, 50% of GOS syrup, vitamins, sweet proteins, and 50% of citric acid are added while stirring. The mixture is poured into pre-oiled molds and allowed to set.

[0358] The list of ingredients and their amounts is shown in Table IE.

[0359] Table IE: list of ingredients

[0360] Example IF: Gummy candy with Brazzein and 50% sugar reduction

[0361] Methods:

[0362] About 50% of the water is heated to 50°C. 50% of the citric acid, all tri sodium, pectin, and half of the FOS and GOS syrups are mixed. The mixture is added to a warm water and heated to 90°C. Sucrose and 50% of FOS syrup are added and the mixture is heated to 110°C and mixed well. Additional water is added and mixed well and then flavor and color additive are added. Then, 50% of GOS syrup, vitamins, sweet protein, and 50% of citric acid are added while stirring. The mixture is poured into pre-oiled molds and allowed to set. The list of ingredients and their amounts is shown in Table IF.

[0363] Table IF: list of ingredients

[0364] Example 1G: Gummy candy with combination of sweet proteins and 50% sugar reduction

[0365] Methods:

[0366] About 50% of the water is heated to 50°C. 50% of the citric acid, all tri sodium, pectin, and half of the FOS and GOS syrups are mixed. The mixture is added to the warm water and heated to 90°C. Sucrose and 50% of FOS syrup are added and the mixture is heated to 110°C and mixed well. Additional water is added and mixed well and then flavor and color additive are added. Then, 50% of GOS syrup, vitamins, sweet proteins, and 50% of citric acid are added while stirring. The mixture is poured into pre-oiled molds and allowed to set.

[0367] The list of ingredients and their amounts is shown in Table 1G.

[0368] TablelG: list of ingredients

[0369] Example 1H: Sugar reduced gummy candy with a combination of sweet proteins

[0370] Methods:

[0371] Maltodextrin, fibers, sucrose, pectin, and modified starch are mixed together, then water is added to the mixture. The aqueous mixture is boiled to 100°C. Then, com syrup is added while stirring and the mixture is heated to 105°C. When reaching this temperature, the mass is cooled down to 60°C. At 60°C sweet protein, food coloring and flavor are added while mixing well, resulting mass of gummy candy. The list of ingredients and their amounts is shown in Table 1H and Table 1J. Table 1H: list of ingredients

[0372] Table 1J: list of ingredients

[0373] Example 2: Sugar reduced Chewable vitamins

[0374] Methods:

[0375] The gum base is heated, and the melted mass is placed in a mixer. All other ingredients listed in Table 2 are added gradually during constant mixing. Then the mass is kneaded to smooth, form and shape the gum.

[0376] The list of ingredients and their amounts is shown in Table 2

[0377] Table 2: list of ingredients for chewable vitamins

[0378] Example 3: Non-carbonated energy drinks

[0379] Methods:

[0380] The ingredients except sweet proteins are weighed and mix well in Thermomix.

[0381] The mixture is heat to a temperature of 90°C. When reaching 90°C, the sweet proteins are added and the mixture is cooled down immediately.

[0382] Example 3A: Energy drink comprising DM31

[0383] The list of ingredients and their amounts is shown in Table 3A.

[0384] Table 3A: list of ingredients for a sugar-reduced energy drink. Example 3B: Energy drink comprising DM137

[0385] The list of ingredients and their amounts is shown in Table 3B.

[0386] Table 3B: list of ingredients for a sugar-reduced energy drink.

[0387] Example 3C: Energy drink comprising DM31 and DM137

[0388] The list of ingredients and their amounts is shown in Table 3C.

[0389] Table 3C: list of ingredients for a sugar-reduced energy drink.

[0390]

[0391] Example 3D: Energy drink comprising Brazzein

[0392] The list of ingredients and their amounts is shown in Table 3D.

[0393] Table 3D: list of ingredients for a sugar-reduced energy drink. Example 3E: Energy drink comprising Brazzein and DM31

[0394] The list of ingredients and their amounts is shown in Table 3E.

[0395] Table 3E: list of ingredients for a sugar-reduced energy drink.

[0396] Example 4: Preparation and characterization of carbonated sugar reduced energy drinks

[0397] Methods:

[0398] All ingredients except the sweet protein / s were added and mixed well until fully dissolved. Then, the sweet protein / s were added and mixed and carbonate was added.

[0399] Sweetness and tastiness evaluation

[0400] Sweetness and tastiness of carbonated energy drinks were determined by a panel of tasters. Each taster rated each tested product with respect to sweetness and tastiness on a scale from 1 to 5. On this scale, the score 1 corresponds to “no noticeable sweetness” or “not tasty”, whereas the score 5 indicates “maximum perceived sweetness” or “very tasty".

[0401] Example 4A: Carbonated energy drink product with DM31 and 40% sugar reduction

[0402] The list of ingredients and their amounts is shown in Table 4A.

[0403] Table 4A: list of ingredients

[0404] *Each vitamin provides 25% of the Daily Value (DV).

[0405] *Each mineral provides 12.25% of the Daily Value (DV).

[0406] Results

[0407] Table 4B shows the tasting results for carbonated energy drink with DM31 with 40% sugar reduction. Table 4B tasting results for carbonated energy drink (with DM31) with 40% sugar reduction

[0408] As can be seen from Table 4B, the drink was perceived as sweet and tasty.

[0409] Example 4B: Carbonated energy drink product with DM137 and 40% sugar reduction

[0410] The list of ingredients and their amounts is shown in Table 4C.

[0411] Table 4C: list of ingredients

[0412] *Each vitamin provides 25% of the Daily Value (DV).

[0413] *Each mineral provides 12.25% of the Daily Value (DV).

[0414] Results

[0415] Table 4D shows the tasting results for carbonated energy drink with 40% sugar reduction.

[0416] Table 4D: Tasting results for carbonated energy drink (with DM137) with 40% sugar reduction

[0417] As can be seen from Table 4D, the energy drink was perceived as both sweet and tasty. Example 4C: Carbonated energy drink product with Brazzein and 40% sugar reduction

[0418] Table 4E shows the components and amount for a carbonated energy drink product with Brazzein and 40% sugar reduction.

[0419] Table 4E: list of ingredients

[0420] *Each vitamin provides 25% of the Daily Value (DV).

[0421] *Each mineral provides 12.25% of the Daily Value (DV).

[0422] Results

[0423] Table 4F shows the tasting results for carbonated energy drink with Brazzein with 40% sugar reduction. Table 4F tasting results for carbonated energy drink (with Brazzein) with 40% sugar reduction

[0424] As can be seen from Table 4F the drink was perceived as sweet and tasty.

[0425] Example 4D: Carbonated energy drink product with Brazzein and DM31 and 40% sugar reduction

[0426] Table 4G shows the components and amount for a carbonated energy drink product with Brazzein and DM31 and 40% sugar reduction.

[0427] Table 4G: list of ingredients

[0428] *Each vitamin provides 25% of the Daily Value (DV). *Each mineral provides 12.25% of the Daily Value (DV).

[0429] Results

[0430] Table 4H shows the tasting results for carbonated energy drink with Brazzein and DM3 1 with 40% sugar reduction.

[0431] Table 4H Tasting results for carbonated energy drink (Brazzein and DM31) with 40% sugar reduction

[0432] As can be seen from Table 4H, the drink was perceived as sweet and tasty. Example 5: Protein bar or Energy bar or snack bar or functional bar

[0433] Example 5A: Energy bar or snack bar or functional bar

[0434] Methods:

[0435] Oatmeal, rice crisped, pecans, and almonds are toasted for 15 minutes at 160°C. Glucose syrup, powdered sugar, sunflower oil, glycerol, lecithin, salt and all other diy ingredients are heated to a homogenous syrup. The toasted pecans and almonds are crushed into small pieces. The dry ingredients (oatmeal, crisped rice, pecans, and almonds) are added to the syrup and mixed. Maltodextrin and sweet protein are added at the end. The mixture is poured into a baking dish. The Granola mix is baked for 10 minutes at 90°C, then it is taken out, cooled down, and stored in a cool place in a closed container.

[0436] The list of ingredients and their amounts is shown in Table 5A.

[0437] Table 5A: list of ingredients

[0438] Example 5B: Energy bar or snack bar or functional bar with 40% sugar reduction

[0439] Methods:

[0440] Oatmeal, pecans, and almonds are roasted for 15 minutes at 160°C. The toasted pecans and almonds are grounded with crisped rice. The glucose syrup, palm oil, glycerol, lecithin and salt are heated while mixing. The grind mixture is combined with whey protein, maltodextrin and sweet protein and is mixed well. The dry ingredients mixture and the actives are poured into hot syrup and are mixed well before transferring to preoiled mold. Then, it is baked for 10 minutes at 90°C.

[0441] The list of ingredients and their amounts is shown in Table 5B.

[0442] Table 5B: list of ingredients for a 40% sugar-reduced bar with sweet protein

[0443] Example 5C: Protein bar or functional bar with 46% sugar reduction comprising

[0444] DM31

[0445] Methods

[0446] Oatmeal, pecans, and almonds were roasted for 15 minutes at 160°C. Pecans, almonds, whole oats and whole crisp rice were toasted and grinded. Glucose syrup, palm oil, glycerol, and lecithin were heated while mixing. Grind mixture, whey protein, maltodextrin, sweet protein were combined and mixed well. Vitamin B (niacin, pantothenic acid, pyridoxine, riboflavin, thiamin, folic acid and cobalamin), Vitamin C (ascorbic acid), taurine, magnesium, potassium and calcium were added and mixed well.

[0447] The dry ingredients mixture and the bioactive compounds (vitamins and minerals) were poured into hot syrup, mixed well before transferring to pre-oiled mold. Then, it was baked for 10 minutes at 90C. The list of ingredients and their amounts is shown in Table 5C.

[0448] Table 5C: list of ingredients for bar with 46% sugar reduction comprising DM31

[0449] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0450] Results

[0451] Table 5D shows tasting results for bar with DM31 with 46% sugar reduction. Table 5D Tasting results for bar (DM31) with 46% sugar reduction

[0452] As can be seen from Table 5D, the bar comprising the sweet protein was perceived as sweet and tasty.

[0453] Example 5D: Protein bar or functional bar comprising DM528 with 46% sugar reduction

[0454] Methods

[0455] Whole Oatmeal, pecans, and almonds were roasted for 15 minutes at 160°C. Pecans, almonds, whole oats and whole crisp rice were toasted and grinded. Glucose syrup, palm oil, glycerol, and lecithin were heated while mixing. Grind mixture, whey protein, maltodextrin, sweet protein were combined and mixed well. Vitamin B (niacin, pantothenic acid, pyridoxine, riboflavin, thiamin, folic acid and cobalamin), Vitamin C (ascorbic acid), taurine, magnesium, potassium and calcium were added and mixed well.

[0456] The dry ingredients mixture and the bioactive compounds (minerals and vitamins) were poured into hot syrup, mixed well before transferring to pre-oiled mold. Then, it was baked for 10 minutes at 90C.

[0457] Table 5E shows the components and amount for bar product (with DM528) with 46% sugar reduction.

[0458] Table 5E: list of ingredients

[0459]

[0460] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0461] Results

[0462] Table 5F shows the tasting results for bar with DM528 with 46% sugar reduction.

[0463] Table 5F Tasting results for bar (DM528) with 46% sugar reduction

[0464]

[0465] As can be seen from Table 5F, the bar was perceived as mildly sweet and tasty.

[0466] Example 5E: Protein bar or functional bar comprising Brazzein with 46% sugar reduction methods:

[0467] Oatmeal, pecans, and almonds were roasted for 15 minutes at 160°C. Pecans, almonds, whole oats and whole crisp rice were toasted and grinded. Glucose syrup, palm oil, glycerol, and lecithin were heated while mixing. Grind mixture, whey protein, maltodextrin, sweet protein were combined and mixed well. Vitamin B (niacin, pantothenic acid, pyridoxine, riboflavin, thiamin, folic acid and cobalamin), Vitamin C (ascorbic acid), taurine, magnesium, potassium and calcium were added and mixed well. The dry ingredients mixture and the bioactive compounds were poured into hot syrup, mixed well before transferring to pre-oiled mold. Then, it was baked for 10 minutes at 90C.

[0468] The list of ingredients and their amounts is shown in Table 5G.

[0469] Table 5G: list of ingredients reduction

[0470] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0471] Results

[0472] Table 5H shows tasting results for bar with Brazzein with 46% sugar reduction.

[0473] Table 5H: tasting results for bar (DM528) with 46% sugar reduction

[0474] As can be seen from Table 5H, the bar was perceived as sweet and tasty.

[0475] Example 6: Protein milk drink

[0476] Example 6A: Protein milk drink comprising DM31

[0477] Methods

[0478] The dry ingredients were weighed and mixed. Liquid components, low -lactose milk, water, and flavor were added and mixed well. Sweet protein DM-3 Iwas added and mixed well.

[0479] The list of ingredients and their amounts is shown in Table 6A and Table 6B.

[0480] Table 6A: list of ingredients for a protein nutrition drink

[0481]

[0482] Table 6B: list of ingredients for a protein nutrition drink with DM31 and 100% sugar reduction

[0483] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0484] Results

[0485] Table 6C shows the tasting results for protein nutrition drink comprising DM3 Iwith 100% sugar reduction.

[0486] Table 6C : tasting results for protein nutrition drink (DM31) with 100% sugar reduction

[0487] As can be seen from Table 6C, the nutrition drink with the sweet protein was perceived as sweet and tasty.

[0488] Example 6B: Protein nutrition drink comprising Brazzein

[0489] Methods:

[0490] The list of ingredients and their amounts is shown in Table 6D.

[0491] Table 6D: list of ingredients

[0492] Example 6C: Protein nutrition drink comprising Brazzein and DM31

[0493] Methods:

[0494] The list of ingredients and their amounts is shown in Table 6E.

[0495] Table 6E: list of ingredients

[0496] Example 6D: Protein nutrition drink comprising Brazzein and DM31 with 100% sugar reduction

[0497] Methods

[0498] The dry ingredients were weighed and mixed. Liquid components: low -lactose milk, water, and flavor were added and mixed well. Sweet protein DM31 and Brazzein were added and mixed well.

[0499] The list of ingredients and their amounts is shown in Table 6F.

[0500] Table 6F: list of ingredients

[0501] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0502] Results

[0503] Table 6G shows the tasting results for protein nutrition drink with Brazzein and DM31 with 100% sugar reduction.

[0504] Table 6G: tasting results for protein nutrition drink (Brazzein and DM31) with 100% sugar reduction As can be seen from Table 6G, the protein nutrition drink was perceived as sweet and tasty.

[0505] Example 6E: Protein nutrition drink comprising DM170 and DM31

[0506] Methods:

[0507] The list of ingredients and their amounts is shown in Table 6H.

[0508] Table 6H: list of ingredients Example 6F: Protein nutrition drink comprising DM137

[0509] Methods:

[0510] The dry ingredients are mixed. Then, the liquid components: low-lactose milk, water, and flavor are added and mixed well. Then, the sweet protein is added and mixed well.

[0511] The list of ingredients and their amounts is shown in Table 61.

[0512] Table 61: list of ingredients

[0513] The list of ingredients and their amounts is shown in Table 6 J. Table 6J: list of ingredients

[0514] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0515] Results

[0516] Table 6K shows the tasting results for protein nutrition drink with DM137 with

[0517] 100% sugar reduction.

[0518] Table 6K: tasting results

[0519] As can be seen from Table 6K the protein nutrition drink with the sweet protein was perceived and sweet and tasty.

[0520] Example 6G: Protein nutrition drink comprising DM137 and DM31 and 100% sugar reduction

[0521] Methods

[0522] The dry ingredients were weighed and mixed. Liquid components: low -lactose milk, water, and flavor were added and mixed well. Sweet protein DM31 and sweet protein DM137 were added and mixed well.

[0523] The list of ingredients and their amounts is shown in Table 6L.

[0524] Table 6L: list of ingredients

[0525] *Each vitamin and mineral provide 25% of the Daily Value (DV).

[0526] Results

[0527] Table 6M shows the tasting results for protein nutrition drink comprising DM137 and DM31 with 100% sugar reduction.

[0528] Table 6M: tasting results for protein nutrition drink (DM137 and DM31) with 100% sugar reduction

[0529] As can be seen from Table 6M the protein nutrition drink with the sweet proteins was perceived and sweet and tasty. Example ?: Sport nutrition drinks

[0530] Example 7A: Sport nutrition drink comprising DM09

[0531] Methods

[0532] All ingredients except sweet protein DM09 are mixed well in Thermomix. The mixture is heated to 90°C. When reaching 90°C, sweet protein DM09 is added and the mixture is allowed to cool down immediately.

[0533] The list of ingredients and their amounts is shown in Table 7A.

[0534] Table 7A: list of ingredients Example 7B: Sport nutrition drink comprising DM31 with 40% sugar reduction

[0535] Methods

[0536] The ingredients except of DM31 were weighted and mixed well until fully dissolved . DM31 was added and mixed . The list of ingredients and their amounts is shown in Table 7B.

[0537] Table 7B: list of ingredients

[0538] *Eachvitammprovides 25% of the Daily Value (DV) ,*Each mineral provides 12.25%of the Daily Value (DV). Results

[0539] Table 7C shows thetasting results for sport nutrition drink comprising DM31 with 40% sugar reduction.

[0540] Table 7C: tasting results for sport nutrition drink (DM31) with 40% sugar reduction

[0541] As can be seen from Table 7C, the sport nutrition drink was perceived as sweet and tasty.

[0542] Example 7C: Sport nutrition drink comprising DM137 with 40% sugar reduction

[0543] Methods

[0544] The ingredients other than DM137 were weighed and mixed well until fully dissolved. DM137 was added and mixed.

[0545] The list of ingredients and their amounts is shown in Table 7D

[0546] Table 7D: list of ingredients

[0547] *Each vitamin provides 25% of the Daily Value (DV) . *Each mineral provides 12.25% of the Daily Value (DV).

[0548] Results

[0549] Table 7E shows thetasting results for sport nutrition drink with DM137 with 40% sugar reduction.

[0550] Table 7E: tasting results for sport nutrition drink (DM137) with 40% sugar reduction

[0551] As can be seen from Table 7E the sport nutrition drink was perceived as sweet and tasty. Example 8: Oral film strip

[0552] Example 8A: Oral strip comprising DM31

[0553] The list of ingredients and their amounts is shown in Table 8A.

[0554] Table 8A: list of ingredients for oral strips. Production Process:

[0555] • A liquid mixture is prepared - temperature and mixing speed are crucial

[0556] • The mixture is spread & dried in drying tunnel by controlling the temperature, air circulation, humidity & coating speed.

[0557] • The dried spread mixture is wound to form a big roll

[0558] • The big roll is unwound to undergo two consecutive cutting phases, where the size of the film strips is selected and the final dosage is defined.

[0559] • The films are punched, pouched and sealed in suitable single-dose sachets.

[0560] The list of ingredients and their amounts is shown in Table 8B.

[0561] Table 8B: list of ingredients Example 8B: Oral strip formulation with no sugar

[0562] Methods:

[0563] An oven was heated to 70°C. The ingredients for the base: water, Tri-sodium citrate, Citric acid, Sodium Alginate, Glycerin, Maltodextrin 2-4, and Xylitol were mixed. Then, sweet protein DM31 and flavors were added, and a casting was prepared. The film was put into the oven for 10 minutes. Strips were cut according to the desired size.

[0564] The list of ingredients and their amounts is shown in Table 8C.

[0565] Table 8C: list of ingredients Example 9: Electrolyte powder comprising sweet proteins

[0566] Example 9A: Electrolyte powder comprising DM31

[0567] Methods

[0568] The ingredients were weighted and mixed well until fully uniform. For each 10 gram of powder, 250 ml of water was added.

[0569] The list of ingredients and their amounts is shown in Table 9A.

[0570] Table 9A: list of ingredients

[0571] *Each vitamin provides 33.33% of the Daily Value (DV) . *Each mineral provides 33.33% of the Daily Value (DV).

[0572] Results

[0573] Table 9B shows the tasting results for electrolyte powder with DM31.

[0574] Table 9B: tasting results for electrolyte powder with DM31

[0575] As can be seen from Table 9B the electrolyte powderwith the sweet protein was perceived as sweet and tasty.

[0576] Example 9B: Electrolyte powder comprising DM31 and Brazzein

[0577] Methods

[0578] The ingredients were weightd and mixed well until fully uniform. For each 10 gram of powder, 250 ml water was added.

[0579] The list of ingredients and their amounts is shown in Table 9C. Table 9C: list of ingredients

[0580] *Each vitamin provides 33.33% of the Daily Value (DV) .

[0581] *Each mineral provides 33.33% of the Daily Value (DV). Results

[0582] Table 9D shows the tasting results for electrolyte powder with DM31 and Brazzein.

[0583] Table 9D: tasting results for electrolyte powder with DM31 and Brazzein

[0584] As can be seen from Table 9D the electrolyte powder with the sweet proteins was perceived as sweet and tasty.

[0585] Example 10: Drink shots Example 11: Tablets

Claims

CLAIMS:

1. A food product comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

2. The food product of claim 1, wherein said bioactive compound is or comprises one or more of a vitamin, a mineral, a botanical extract, a phytochemical, an amino acid, a probiotic, a prebiotic, a postbiotic, a peptide, a protein, a nucleotide, a nucleoside, a polyphenol, a flavonoid, a carotenoid, an alkaloid, a glycoside, a lipid, an essential fatty acid, an antioxidant, an enzyme, a metabolite, an electrolyte, a stimulant, a fiber or any combination thereof.

3. The food product of claim 1 or 2, wherein said bioactive compound is one or more of a vitamin, a mineral, an antioxidant, caffeine, taurine, a fiber or any combination thereof.

4. The food product of any one of claims 1 to 3, wherein said vitamin is one or more of vitamin C, vitamin B, vitamin A, vitamin D, vitamin E, vitamin K or any combination thereof.

5. The food product of claim 4, wherein said vitamin B is one or more of vitamin B 1 (Thiamine), vitamin B2 (Riboflavin), vitamin B3 (Niacin), vitamin B5 (Pantothenic acid), vitamin B6 (Pyridoxine), vitamin B7 (Biotin), vitamin B9 (Folic Acid), vitamin B12 (Cobalamin) or any combination thereof.

6. The food product of any one of claims 1 to 3, wherein said mineral is a mineral salt.

7. The food product of claim 6, wherein said mineral salt is one or more of a is a magnesium salt, a potassium salt, a calcium salt, a sodium salt or any combination thereof.

8. The food product of any one of claims 1 to 3, wherein said fiber is inulin, fructooligosaccharides (FOS), Galactooligosaccharides (GOS) or any combination thereof.

9. The food product of any one of claims 1 to 8, comprising at least 20% of a daily recommended value of said at least one bioactive compound.

10. The food product of any one of claims 1 to 9, wherein said sweet protein is at least one protein selected from the group consisting of Monellin, Thaumatin, Miraculin, Curculin, Brazzein, Mabinlin, sweet protein from honey truffle or a variant or combination thereof.

11. The food product of any one of claim 1 to 10, wherein said sweet protein is or comprises one or more proteins denoted by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO :9, SEQ ID NO : 10, SEQ ID NO : 11 , SEQ ID NO : 12, SEQ ID NO : 13 , SEQ ID NO :352, a variant thereof or any combinations thereof.

12. The food product of any one of claim 1 to 9, wherein said sweet protein is or comprises a protein denoted by SEQ ID NO: 14, SEQ ID NO: 350, a variant thereof or a combination thereof.

13. The food product of any one of claim 1 to 9, wherein said at least one sweet protein is or comprises one or more proteins denoted by SEQ ID NO: 14- SEQ ID NO:351, SEQ ID NO:353-SEQ ID NO:479, a variant thereof or any combination thereof.

14. The food product of any one of any one of claims 1 to 13, wherein said at least one sweet protein is or comprises one or more proteins denoted by SEQ ID NO: 16, SEQ ID N0:17, SEQ ID NO:39, SEQ ID NO:53, SEQ ID NO:104, SEQ ID NO:186, SEQ ID NO:217, SEQ ID NO:263, SEQ ID NO:257, SEQ ID NO:351, a variant thereof or any combinations thereof.

15. The food product of any one of claims 1 to 14, wherein said at least one sweet protein is at a concentration of at least about 0.00001 / w%.

16. The food product of any one of claims 1 to 15, comprising stevia or any derivative thereof.

17. The food product of claim 16, wherein said stevia or any derivative thereof is or comprises at least one of steviol glycoside, Rebaudioside A (Reb A), Rebaudioside M (Reb M), Rebaudioside D (Reb D), Stevioside, Rebaudioside or a combination thereof.

18. The food product of any one of claims 1 to 15, comprising one or more sweetener selected from the group consisting of at least one sugar, high intensity sweetener, a natural sweetener, a sugar alcohol, a rare sugar, an amino acid or a combination thereof.

19. The food product of claim 18, wherein said sugar is one or more of sucrose, glucose, lactose, fructose, palatinose or a combination thereof.

20. The food product of claim 18, wherein said high intensity sweetener is one or more of saccharin, aspartame, acesulfame, potassium (ace-k), neotame, advantame or a combination thereof.

21. The food product of claim 18, wherein said natural sweetener is one or more of monk fruit, mycodulcein, glycine, honey, agave, maple syrup, Silan, black treacle, molasses or a combination thereof.

22. The food product of claim 18, wherein said sugar alcohol is one or more of mannitol, maltitol, sorbitol, xylitol, lactitol, isomalt, erythritol or a combination thereof.

23. The food product of claim 18, wherein said rare sugar is one or more of allulose, turanose, isomaltulose, isomaltose, tagarose or a combination thereof.

24. The food product of any one of claims 1 to 23, comprising one or more non-tastemodulating proteins.

25. The food product of claim 24, comprising said non-taste-modulating protein is one or more of whey protein, a casein protein, a soy protein, a pea protein, a rice protein, a chickpea protein or any combination thereof.

26. The food product of any one of claims 1 to 25, being a dietary supplement.

27. The food product of any one of claims 1 to 25, being a functional food.

28. The food product of any one of claims 1 to 25, being a gummy candy, a chewable product, a liquid, a bar, an edible film strip, a hard candy, a powder.

29. The food product of claim 28, wherein said chewable product is one or more of a chewable vitamin, a chewable candy, a chewing gum or any combination thereof.

30. The food product of claim 28, wherein said liquid is a sports drink, an energy drink, a carbonated energy drink, a sport nutrition drink, a protein milk drink, a functional beverage, a drink shot, a shake, a hydration, a liquid gel or a combination thereof.

31. The food product of claim 28, wherein said bar is a protein bar, an energy bar, a snack bar, a functional bar or any combination thereof.

32. The food product of claim 31, wherein said bar is a protein bar.

33. The food product of claim 28, wherein said edible film strip is an oral thin film strip or edible film.

34. The food product of claim 28, wherein said powder is for reconstituting as a drink, a meal, a shake, a capsule.

35. The food product of claim 28, wherein said powder is an electrolyte powder.

36. The food product of any one of claims 1 to 35, characterized by a similar sweetness level as a food product essentially free of the one or more sweet proteins.

37. The food product of any one of claims 1 to 35, characterized by a reduced sweetness level as a food product essentially free of the one or more sweet proteins.

38. A dietary supplement comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

39. A functional food comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

40. A gummy candy comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

41. A chewable vitamin comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

42. A liquid comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

43. A carbonated energy drink comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

44. A protein bar and / or an energy bar comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

45. A sport nutrition drink comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

46. A protein milk drink comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

47. An oral film strip comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

48. An electrolyte powder comprising one or more bioactive compounds and one or more sweeteners, wherein said one or more sweeteners is or comprises at least one sweet protein.

Images