Modifications of transgenic corn event mon87429, methods for modifying transgenic corn event mon87429, and uses thereof

Abstract

The present disclosure provides modified versions of transgenic com event MON87429, as well as plants, plant cells, seeds, plant parts (including pollen, seed, and cells, and tissues corresponding to tassel, root, stalk, stem, leaf, cobb, and the like), progeny plants, and commodity products comprising the modified versions of corn event MON87429 DNA. Polynucleotides specific for a modified com event MON87429 and methods for detecting a modified corn event MON87429 are also provided. Methods for making and using plants, plant cells, seeds, plant parts, progeny plants, and commodity products comprising a modified corn event MON87429 and detecting a modified corn event MON 87429 are also provided.

Description

TITLE OF THE INVENTION MODIFICATIONS OF TRANSGENIC CORN EVENT MON87429, METHODS FOR MODIFYING TRANSGENIC CORN EVENT MON87429, AND USES THEREOF CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application claims the benefit of priority of U.S. Provisional Application No. 63 / 568,118, filed March 21, 2024, the disclosure of which is hereby incorporated by reference in its entirety. INCORPORATION OF SEQUENCE LISTING

[0002] The sequence listing contained in the file named MONS574WO_ST26.xml is 270 kilobytes (measured in Microsoft Windows®), was created on February 6, 2025, is filed herewith by electronic submission, and is incorporated by reference. FIELD

[0003] The present disclosure relates to modifications of recombinant DNA molecules present in and / or isolated from corn event MON87429. The present disclosure also relates to transgenic corn plants, plant parts, and seed, pollen, cells, and agricultural products containing modifications of corn event MON87429, as well as methods of producing, modifying and using the same, detecting the presence of modified corn event MON87429 in samples derived from a corn plant, plant part, plant seed, plant tissue, or plant cell. The present disclosure also relates to methods of controlling weeds using modified corn event MON87429. Transgenic corn plants, plant parts, seeds and cells comprising corn event MON87429 or modified corn event MON87429 exhibit tolerance to inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group, synthetic auxins, inhibitors of glutamine synthetase, and inhibitors of 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS). BACKGROUND

[0004] Corn (Zea mays) is an important crop and is a primary food source in many areas of the world. The methods of biotechnology have been applied to corn for improving agronomic traits1 US_ACTIVE\129309744\V-1and product quality. One such agronomic trait is herbicide tolerance, manifested through the insertion of a recombinant DNA segment into the genome of the corn plant.

[0005] Transgenic corn traits have been used to impart tolerance to glyphosate, glufosinate, and 2,4-D and are used broadly in commercial corn production for weed management. However, weeds have evolved resistance to herbicides and weed resistance continues to present a challenge in corn production today. Therefore, there is a continuing need in the art to provide novel transgenic corn events that exhibit additional herbicide tolerance trait options to manage weeds effectively and to sustain crop productivity. The present disclosure provides examples of novel modifications to a transgenic corn event that provides multiple modes of action for tolerance to different herbicides. SUMMARY

[0006] A recombinant DNA molecule is provided. The recombinant DNA molecule comprises: (a) a first nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO: 9; and (b) a second nucleotide sequence that (i) comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16; or (ii) is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16; or (iii) is selected from the group consisting of SEQ ID NOs:18-117. The recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10. The2 US_ACTIVE\129309744\V-1recombinant DNA molecule can further comprise: (c) a third nucleotide sequence that comprises: (i) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or (ii) is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17; or (iii) is selected from the group consisting of SEQ ID NOs:118-217. The recombinant DNA molecule can have a deletion of one or more consecutive nucleotides between the second nucleotide sequence and the third nucleotide sequence relative to SEQ ID NO:10, 15, or 17. Alternatively or in addition, the recombinant DNA molecule can have a deletion of one or more consecutive nucleotides between the first nucleotide sequence and the second nucleotide sequence relative to SEQ ID NO:10, 14, or 16.

[0007] Another recombinant DNA molecule is provided. The recombinant DNA molecule comprises: (a) a first nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9; and (b) a second nucleotide sequence that: (i) comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or (ii) is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least3 US_ACTIVE\129309744\V-112, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or (iii) is selected from the group consisting of SEQ ID NOs:118-217. The recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10. The recombinant DNA molecule can have a deletion of one or more consecutive nucleotides between the first nucleotide sequence and the second nucleotide sequence relative to SEQ ID NO:10, 15, or 17.

[0008] Yet another recombinant DNA molecule is provided. The recombinant DNA molecule comprises a first nucleotide sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the full length of SEQ ID NO: 9. The recombinant DNA molecule further comprises at the 5’ or 3’ end of said first nucleotide sequence a second nucleotide sequence comprising: (a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16; and / or (b) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17. The recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10. The recombinant DNA molecule can comprise at the 5’ end of said first nucleotide sequence one or more nucleotide sequences selected from SEQ ID NOs:18–117. Alternatively, or in addition, the4 US_ACTIVE\129309744\V-1recombinant DNA molecule can comprise at the 3’ end of said first nucleotide sequence one or more nucleotide sequences selected from SEQ ID NOs:118–217.

[0009] Any of the recombinant DNA molecules provided herein can be comprised in a corn plant, corn plant part, corn plant cell, corn plant seed, corn progeny plant, or commodity or fuel product made from corn and corn plant parts.

[0010] Any of the recombinant DNA molecules provided herein can comprise an amplicon diagnostic for the presence of DNA comprising a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9.

[0011] Any of the recombinant DNA molecules provided herein can be derived from a corn plant, corn plant part, corn seed, processed corn seed, corn plant cell or tissue, animal feed comprising corn, corn oil, corn meal, corn flour, corn flakes, corn bran, food made comprising corn, corn biomass, or fuel products made from corn and corn plant parts.

[0012] Another recombinant DNA molecule is provided. The recombinant DNA molecule comprises a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with a polynucleotide of a modified corn event MON87429 DNA in a sample. The polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and complete complements of any thereof. Detecting hybridization of the polynucleotide under the stringent hybridization conditions is diagnostic for the presence of the modified corn event MON87429.

[0013] A pair of DNA molecules is provided. The pair of DNA molecules comprises a first DNA molecule and a second DNA molecule that function as DNA primers when used together in an amplification reaction with DNA comprising a corn modified event MON87429 to produce an amplicon diagnostic for the modified corn event MON87429 in a sample. The first DNA molecule and the second DNA molecule are different from each other. The first DNA molecule is: (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100%5 US_ACTIVE\129309744\V-1identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof, or (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement of any thereof. The second DNA molecule is: (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof, or (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement of any thereof, or (iii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at6 US_ACTIVE\129309744\V-1least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement of any thereof. The amplicon can comprise a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10.

[0014] A method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample is provided. The method comprises: (a) contacting said sample with any the recombinant DNA molecules that function as probes that hybridize specifically under stringent hybridization conditions with a polynucleotide of a modified corn event MON87429; (b) subjecting said sample and said recombinant DNA molecule to stringent hybridization conditions; and (c) detecting hybridization of said recombinant DNA molecule to said DNA segment in said sample. Detection is diagnostic for the presence of said modified corn event MON87429 DNA in said sample.

[0015] Another method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample is provided. The method comprises: (a) contacting said sample with any of the pairs of DNA molecules that function as DNA primers when used together in an amplification reaction with DNA comprising a modified corn event MON87429 to produce an amplicon diagnostic for the modified corn event MON87429; (b) performing an amplification reaction sufficient to produce a DNA amplicon; and (c) detecting the presence of said DNA amplicon in said reaction. The presence of said DNA amplicon is diagnostic for the presence of said modified corn event MON87429 DNA in said sample.

[0016] Yet another method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample is provided. The method comprises performing a sequencing reaction to produce a target nucleotide sequence, wherein said target nucleotide sequence comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof.

[0017] A modified corn plant, corn plant part, corn seed, or corn cell is provided. The modified corn plant, corn plant part, corn seed, or corn cell comprises a modified corn event MON87429.7 US_ACTIVE\129309744\V-1The modified corn plant, corn plant part, corn seed, or corn cell can comprise: (a) any of the recombinant DNA molecules provided herein; or (b) a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a complement thereof; or (c) a DNA molecule comprising a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17, or a complement of any thereof. Any of the modified corn plants, corn plant parts, corn seeds, or corn cells can be further defined as a progeny plant of any generation of a corn plant comprising a modified corn event MON87429, or a corn plant part, corn seed, or corn cell derived therefrom. Any of the modified corn plants, corn plant parts, corn seeds, or corn cells can exhibit tolerance to one or more herbicides selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof.

[0018] A DNA detection kit is provided. The kit can comprise: (a) any the recombinant DNA molecules that function as probes that hybridize specifically under stringent hybridization conditions with a polynucleotide of a modified corn event MON87429; or (b) any of the pairs of DNA molecules that function as DNA primers when used together in an amplification reaction with DNA comprising a modified corn event MON87429 to produce an amplicon diagnostic for the modified corn event MON87429.8 US_ACTIVE\129309744\V-1

[0019] A method of producing a progeny corn plant comprising a modified corn event MON87429 is provided. The method comprises: (a) sexually crossing a first corn plant that comprises the modified corn event MON87429 with itself or a second corn plant; (b) collecting one or more seeds produced from said cross; (c) growing said seed to produce one or more progeny plants; and (d) selecting at least a first progeny plant or seed comprising the modified corn event MON87429. Selecting at least a first progeny plant or seed comprising the modified corn event MON87429 can comprise treating the progeny plant or seed with an effective amount of at least one herbicide selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5- enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof. The method can further comprise (e) collecting seed from said at least first progeny plant comprising the modified corn event MON87429.

[0020] A hybrid modified corn plant or seed comprising a modified corn event MON87429 is provided. The hybrid modified corn plant or seed can be produced by any of the methods of producing a progeny corn plant comprising a modified corn event MON87429 described herein.

[0021] A nonliving corn plant material is provided. The nonliving corn plant material comprises a detectable amount of any of the recombinant DNA molecules provided herein.

[0022] A microorganism is provided. The microorganism comprises any of the recombinant DNA molecules provided herein.

[0023] A plant cell is provided. The plant cell comprises any of the recombinant DNA molecules provided herein.

[0024] A commodity product is provided. The commodity product comprises any of the recombinant DNA molecules provided herein. The commodity product can be produced from a modified corn plant, corn plant part, corn seed, or corn tissue or cell comprising a modified corn event MON87429. The commodity product can be selected from the group consisting of whole or processed corn seed; food for human consumption or animal feed such as corn oil, corn meal, corn flour, corn grits, corn flakes, corn bran, or corn starch; sweetener such as high fructose corn syrup (HFCS), glucose or dextrose; beverage alcohol; brewer grits for beer production; fiber; corn9 US_ACTIVE\129309744\V-1biomass; fuel products produced using corn and corn plant parts; corn pollen; corn plastic; dried distillers grains (DDGs); and bio-degradable packing material.

[0025] A method of producing a commodity product is provided. The method comprises: (a)obtaining a modified corn plant, corn plant part, or corn seed comprising a modified corn event MON87429; and (b) producing a commodity product from the modified corn plant, corn plant part, or corn seed.

[0026] A corn plant, corn plant part, or corn seed is provided. The corn plant, corn plant part, or corn seed comprises a DNA molecule or segment functional as a template when tested in a DNA amplification method to produce an amplicon diagnostic for the presence of a modified corn event MON87429 DNA.

[0027] A method of determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 is provided. The method comprises: (a) contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a first primer pair that can produce a first amplicon of all or part of the modified corn event MON87429 and a second primer pair that can produce a second amplicon of a standard genomic sequence known to be single copy and homozygous in the corn plant, corn plant part, or corn seed; (b) contacting the sample with a first probe that specifically hybridizes to the first amplicon and / or all or part of the modified corn event MON87429, and a second probe that specifically hybridizes to the standard genomic sequence; (c) performing a DNA amplification reaction using real-time PCR with the sample and determining the cycle thresholds (Ct values) of the first amplicon and the second amplicon; (d) calculating the difference (ΔCt) between the Ct values of the second amplicon and the first amplicon; and (e) determining the zygosity of the modified corn event MON87429, wherein a ΔCt of about zero (0) indicates homozygosity of the modified corn event MON87429 and a ΔCt of about one (1) indicates heterozygosity of the modified corn event MON87429.

[0028] Another method of determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 is provided. The method comprises: (a) contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a primer pair capable of producing a first amplicon diagnostic for the modified corn event MON87429 and a second amplicon diagnostic for native corn genomic DNA not comprising the modified corn event MON87429; (b) performing a nucleic acid amplification reaction with the sample and the set of10 US_ACTIVE\129309744\V-1primer pairs; and (c) detecting the first amplicon and the second amplicon. The presence of only the first amplicon is diagnostic of a corn plant, corn plant part, or corn seed homozygous for the modified corn event MON87429. The presence of only the second amplicon is diagnostic of a corn plant, corn plant part, or corn seed homozygous for native corn genomic DNA not comprising the modified corn event MON87429. The presence of both the first amplicon and the second amplicon is diagnostic of a corn plant, corn plant part, or corn seed heterozygous for the modified corn event MON87429.

[0029] Yet another method for determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 is provided. The method comprises: (a)\ contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a probe set which contains at least a first probe that specifically hybridizes to the modified corn event MON87429 and at least a second probe that specifically hybridizes to corn genomic DNA that was disrupted by insertion of the heterologous DNA of corn event MON87429 and is disrupted by the modified corn event MON87429 DNA, wherein the second probe does not hybridize to the modified corn event MON87429 DNA; and (b) hybridizing the probe set with the sample under stringent hybridization conditions. Detecting hybridization of only the first probe under the hybridization conditions is diagnostic for a corn plant, corn plant part, or corn seed homozygous for the modified corn event MON87429. Detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a corn plant, corn plant part, or corn seed heterozygous for the modified corn event MON87429.

[0030] A population of transgenic corn plants is provided. Each transgenic corn plant comprises a modified corn event MON87429. Each transgenic corn plant of the population can exhibit tolerance to at least one herbicide selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof.

[0031] A method of modifying a corn plant comprising event MON87429 is provided. The method comprises: (a) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an11 US_ACTIVE\129309744\V-1explant of a corn plant comprising corn event MON87429, or a plant part thereof, to produce a modified corn event MON87429 via a genome editing technique; and (b) developing or regenerating a modified corn plant comprising the modified corn event MON87429. The site- specific nuclease can be a zinc-finger nuclease (ZFN), a meganuclease, an RNA-guided endonuclease, a TALE-endonuclease (TALEN), a recombinase, or a transposase. For example, the site-specific nuclease can be a CRISPR / Cas nuclease.

[0032] In any of the methods of modifying a corn plant comprising event MON87429 where the site-specific nuclease is a CRISPR / Cas nuclease, the introducing step (a) can comprise introducing the recombinant DNA construct into the at least one cell of the explant, wherein the recombinant DNA construct further comprises an expression cassette encoding a first guide RNA (gRNA). The recombinant DNA construct can further comprise an expression cassette encoding a second guide RNA (gRNA).

[0033] In any of the methods of modifying a corn plant comprising event MON87429 where the site-specific nuclease is a CRISPR / Cas nuclease, the introducing step (a) can further comprise introducing a first guide RNA (gRNA) or a second recombinant DNA construct comprising an expression cassette encoding a first guide RNA (gRNA) into the at least one cell of the explant. The second recombinant DNA construct can further comprise an expression cassette encoding a second guide RNA (gRNA).

[0034] In any of the methods of modifying a corn plant comprising event MON87429 where the site-specific nuclease is a CRISPR / Cas nuclease, the introducing step (a) can comprise introducing at least two guide RNAs (gRNAs) comprising a first gRNA and a second gRNA into the at least one cell of the explant.

[0035] In any of the methods of modifying a corn plant comprising event MON87429 where the site-specific nuclease is a CRISPR / Cas nuclease, the introducing step (a) can comprise introducing a first gRNA and second recombinant DNA construct comprising an expression cassette encoding a second guide RNA (gRNA) into the at least one cell of the explant.

[0036] In any of the methods of modifying a corn plant comprising event MON87429, the site- specific nuclease can have a first target site in the genome of the corn plant at or near corn event MON87429. The site-specific nuclease can additionally have a second target site in the genome of the corn plant at or near corn event MON87429.12 US_ACTIVE\129309744\V-1

[0037] In any of the methods of modifying a corn plant comprising event MON87429, the introducing step (a) can further comprise introducing a second site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a second site specific nuclease into at least one cell of the explant. The second site-specific nuclease has a second target site in the genome of the corn plant at or near corn event MON87429.

[0038] In any of the methods of modifying a corn plant comprising event MON87429 that involve the use of a first gRNA, the first gRNA can have a first target site in a flanking DNA sequence, 5´ flank, 3´ flank, junction sequence, or T-DNA insertion sequence of corn event MON87429, or a complement thereof. For example, the first gRNA can have a first target site comprising a target sequence that is: (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or (iii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.13 US_ACTIVE\129309744\V-1

[0039] In any of the methods of modifying a corn plant comprising event MON87429 that involve the use of a first gRNA, the first gRNA can have a second target site comprising a target sequence that is: (i) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or (ii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or (iii) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.

[0040] In any of the methods of modifying a corn plant comprising event MON87429 that involve the use of a first gRNA and a second gRNA, the first gRNA can have a first target site comprising a target sequence that is: (a) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides14 US_ACTIVE\129309744\V-1of SEQ ID NO:14 or 16, or a complement thereof; or (b) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or (c) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof. The second gRNA can have a second target site comprising a target sequence that is: (a) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or (b) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or (c) at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or15 US_ACTIVE\129309744\V-1100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.

[0041] In any of the methods of modifying a corn plant comprising event MON87429 where there are first and second target sites, the modified corn event MON87429 can comprise a deletion or excision of intervening DNA between the first target site and the second target site, relative to the corn event MON87429.

[0042] In any of the methods of modifying a corn plant comprising event MON87429, the method can further comprise: (c) selecting the modified corn plant comprising the modified corn event MON87429; and (d) sexually crossing the modified corn plant with itself or a second corn plant to produce one or more modified progeny corn plants.

[0043] A method of introducing a target site into a corn plant is provided. The method comprises: (a) introducing a cognate target site into the corn event MON87429 locus of at least one cell of a corn plant or corn plant part comprising the corn event MON87429 or an explant thereof via a targeted genome editing technique, wherein the cognate target site is identical or similar to an originator target site for a site-specific nuclease present in the corn event MON87429 locus; and (b) developing or regenerating a modified corn plant comprising a modified corn event MON87429 comprising the cognate target site. The method can further comprise: (c) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a corn plant comprising the modified corn event MON87429 or a plant part thereof, to produce a further modified corn event MON87429 via a targeted genome editing technique, wherein the target site of the site-specific nuclease includes the cognate target site and the originator target site; and (d) developing or regenerating a second modified corn plant comprising the further modified corn event MON87429.

[0044] Another method of introducing a target site into a corn plant is provided. The method comprises: (a) introducing a cognate target site into the corn event MON87429 locus of at least one cell of a corn plant or corn plant part comprising the corn event MON87429 or an explant thereof via a targeted genome editing technique to produce a modified corn event MON8742916 US_ACTIVE\129309744\V-1comprising the cognate target site, wherein the cognate target site is identical or similar to an originator target site for a site-specific nuclease present in the corn event MON87429 locus, and (b) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a modified corn plant comprising the modified corn event MON87429, or a plant part thereof, to produce a further modified corn event MON87429 via a targeted genome editing technique, wherein the target site of the site-specific nuclease includes the cognate target site and the originator target site; and (c) developing or regenerating a second modified corn plant comprising the further modified corn event MON87429.

[0045] In any of the methods of introducing a target site into a corn plant involving development or regeneration of a second modified corn plant, the further modified corn event MON87429 of the second modified corn plant can comprise a deletion or excision of intervening DNA between the originator target site and the cognate target site, relative to the corn event MON87429 or modified corn event MON87429.

[0046] In any of the methods of introducing a target site into a corn plant involving development or regeneration of a second modified corn plant, the method can further comprise selecting the second modified corn plant or a progeny plant of the second modified corn plant comprising the further modified corn event MON87429, and sexually crossing the second modified corn plant or the progeny plant with itself or another corn plant to produce one or more modified progeny corn plants comprising the further modified corn event MON87429. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 is a diagrammatic depiction of the orientation and alignment of the DNA elements or sequences present within the nucleotide sequence shown in SEQ ID NO:10, which represents the sequence of the inserted transgenic DNA and the corresponding adjacent 5’ and 3’ flanking corn genomic sequence present in the corn event MON87429. Horizontal lines correspond to the approximate positions of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9 relative to SEQ ID NO:10. The horizontal arrows referred to as SQ51062 (SEQ ID NO:11) and SQ51053 (SEQ ID NO:12) represent the approximate positions of illustrative primer pair that can be used to detect corn event MON87429. The horizontal line referred to as PB50370 (SEQ ID NO:13) represents the17 US_ACTIVE\129309744\V-1approximate position of an illustrative DNA probe that can be used to detect corn event MON87429. “RB” refers to the Agrobacterium T-DNA right border; “LB” refers to the Agrobacterium T-DNA left border. “P” represents a promoter element; “L” represents a leader (5´ UTR) element; “I” represents an intron element; “TS” represents a chloroplast transit peptide element; “T” represents a 3´ UTR element; “PAT” represents a phosphinothricin N- acetyltransferase coding element; “DMO” represents a dicamba monooxygenase coding element; “FT” represents a FT_T coding element; CP4-EPSPS represents a 5-enolpyruvylshikimate-3- phosphate synthase codling sequence; mts-siRNA represents a sequence of a male tissue specific siRNA target.

[0048] Figure 2 illustrates the approximate timeline for the research, testing, and development, leading to selection of the commercial corn event MON87429. “PAT” refers to phosphinothricin N-acetyltransferase; “DMO” refers to dicamba monooxygenase; “FT” refers to FT_T; CP4-EPSPS refers to 5-enolpyruvylshikimate-3-phosphate synthase; mts-siRNA refers to a male tissue specific siRNA target. BRIEF DESCRIPTION OF THE SEQUENCES

[0049] SEQ ID NO:1 is a 30-nucleotide sequence representing the 5´ junction region of corn genomic DNA and the transgenic insert, and corresponds to nucleotide positions 1,015-1,044 of SEQ ID NO:10.

[0050] SEQ ID NO:2 is a 30-nucleotide sequence representing the 3´ junction region of the transgenic insert and the corn genomic DNA, and corresponds to nucleotide positions 15,023- 15,052 of SEQ ID NO:10.

[0051] SEQ ID NO:3 is a 60-nucleotide sequence representing the 5´ junction region of corn genomic DNA and the transgenic insert, and corresponds to nucleotide positions 1,000-1,059 of SEQ ID NO:10.

[0052] SEQ ID NO:4 is a 60-nucleotide sequence representing the 3´ junction region of the transgenic insert and the corn genomic DNA, and corresponds to nucleotide positions 15,008- 15,067 of SEQ ID NO:10.

[0053] SEQ ID NO:5 is a 100-nucleotide sequence representing the 5´ junction region of corn genomic DNA and the transgenic insert, and corresponds to nucleotide positions 980-1,079 of SEQ ID NO:10.18 US_ACTIVE\129309744\V-1

[0054] SEQ ID NO:6 is a 100-nucleotide sequence representing the 3´ junction region of the transgenic insert and the corn genomic DNA, and corresponds to nucleotide positions 14,988- 15,087 of SEQ ID NO:10.

[0055] SEQ ID NO:7 is a 1,350-nucleotide sequence representing 1029 nucleotides of the 5´ flanking corn genomic DNA and the 321 nucleotides of the 5’ end of the transgenic insert. It corresponds to nucleotide positions 1-1,350 of SEQ ID NO:10.

[0056] SEQ ID NO:8 is a 1,069-nucleotide sequence representing 38 nucleotides of the 3’ end of the transgenic insert and 1031 nucleotides of the 3’ flanking corn genomic sequences. It corresponds to nucleotide positions 15,000-16,068 of SEQ ID NO:10.

[0057] SEQ ID NO:9 is a 14,008-nucleotide sequence of the transgenic insert of the corn event MON87429. It corresponds to nucleotide positions of 1,030-15,037 of SEQ ID NO:10.

[0058] SEQ ID NO:10 is a 16,068-nucleotide sequence of the corn event MON87429. The sequence contains the nucleotide sequence of the 5´ flanking genomic DNA sequence (nucleotides 1-1000), a stretch of 29 nucleotides inserted during T-DNA insertion (nucleotides 1001-1029), the transgenic DNA insert (nucleotides 1,030-15,037), a stretch of 31 nucleotides inserted during T- DNA insertion (nucleotides 15,038-15,068) and the 3´ flanking genomic DNA sequence (nucleotides 15,069-16,068). For simplicity, the 5´ flanking genomic DNA sequence (nucleotides 1-1000) and the stretch of 29 nucleotides inserted during T-DNA insertion (nucleotides 1001- 1029), and the stretch of 31 nucleotides inserted during T-DNA insertion (nucleotides 15,038- 15,068) and the 3´ flanking genomic DNA sequence (nucleotides 15,069-16,068) are referred to as the 5’ flanking genomic DNA sequence and the 3’ flanking genomic DNA sequence, respectively.

[0059] SEQ ID NO:11 is a 29-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51062 used to identify corn event MON87429 DNA in a sample. It is identical to the reverse complement of the nucleotide sequence corresponding to positions 15,038- 15,066 of SEQ ID NO:10.

[0060] SEQ ID NO:12 is a 17-nucleotide sequence corresponding to a thermal amplification primer referred to as SQ51053 used to identify corn event MON87429 DNA in a sample. It corresponds to positions 14,987-15,003 of SEQ ID NO:10.19 US_ACTIVE\129309744\V-1

[0061] SEQ ID NO:13 is a 16-nucleotide sequence corresponding to a probe referred to as PB50370 used to identify corn event MON87429 DNA in a sample. It corresponds to positions 15,009-15,024 of SEQ ID NO:10.

[0062] SEQ ID NO:14 is a 1,029-nucleotide sequence representing the 5’ flanking corn genomic DNA (nucleotides 1-1,000) plus 29 nucleotides (nucleotides 1,001-1,029) inserted during T-DNA insertion up to the transgene insert (SEQ ID NO:9). SEQ ID NO:14 corresponds to nucleotide positions 1-1,029 of SEQ ID NO:10.

[0063] SEQ ID NO:15 is a 1,031-nucleotide sequence representing 31 nucleotides inserted during T-DNA insertion (nucleotides 15,038-15,068) and the 3’ flanking corn genomic DNA after the transgene insert (SEQ ID NO:9). SEQ ID NO:15 corresponds to nucleotide positions 15,038- 16,068 of SEQ ID NO:10.

[0064] SEQ ID NO:16 is a 5000-nucleotide sequence representing corn genomic DNA that flanks the transgenic insert at the 5´end of the insert. Nucleotides 4,001-5,000 of SEQ ID NO:16 are identical to nucleotides 1-1,000 of SEQ ID NO:14. Nucleotides 1-4000 are based on the genomic sequence of the corn B73 germplasm.

[0065] SEQ ID NO:17 is a 5,000-nucleotide sequence representing corn genomic DNA that flanks the transgenic insert at the 3´ end of the insert. Nucleotides 1-1,000 of SEQ ID NO:17 are identical to nucleotides 32-1,031 of SEQ ID NO:15. The remaining nucleotides (1,001-5,000) are based on the genomic sequence of the corn B73 corn germplasm.

[0066] SEQ ID NOs:18–117 are 50-nucleotide sequences in the 5’ flank genomic sequence of event MON87429, not including the 29 nucleotides inserted during T-DNA insertion. SEQ ID NOs:18–97 are based on the genomic sequence of corn B73 germplasm; SEQ ID NOs:98–117 are based on the genomic sequence of the transformation germplasm and are identical to the corresponding sequences of B73 germplasm.

[0067] SEQ ID NOs:118–217 are 50-nucleotide sequences in the 3’ flank genomic sequence of event MON87429, not including the 31 nucleotides inserted during T-DNA insertion. SEQ ID NOs:118–137 are based on the genomic sequence of the transformation germplasm and are identical to the corresponding sequences of B73 germplasm; SEQ ID NOs:138–217 are based on the genomic sequence of corn B73 germplasm.

[0068] SEQ ID NOs:218 and 219 are the codon-optimized nucleotide and amino acid sequences of FnCas12a (also known as FnCpf1) nuclease of Francisella novicida, respectively.20 US_ACTIVE\129309744\V-1

[0069] SEQ ID NOs:220 and 221 are the codon-optimized nucleotide and amino acid sequences of LbCas12a (also known as LbCpf1) nuclease of Lachnospiraceae bacterium ND2006, respectively.

[0070] SEQ ID NO:222 is the amino acid sequence of LbCas12a-TYC nuclease.

[0071] SEQ ID NO:223 is the amino acid sequence of LbCas12a-TAT nuclease.

[0072] SEQ ID NO:224 is a 27-nucleotide sequence corresponding to an OgRRS 5F-41.

[0073] SEQ ID NO:225 is a 27-nucleotide sequence corresponding to an OgRRS 5F-43.

[0074] SEQ ID NO:226 is a 27-nucleotide sequence corresponding to an OgRRS 5J-1.

[0075] SEQ ID NO:227 is a 27-nucleotide sequence corresponding to an OgRRS TI-532.

[0076] SEQ ID NO:228 is a 27-nucleotide sequence corresponding to an OgRRS TI-1025.

[0077] SEQ ID NO:229 is a 27-nucleotide sequence corresponding to an OgRRS TI-1676.

[0078] SEQ ID NO:230 is a 27-nucleotide sequence corresponding to an OgRRS 3J-1.

[0079] SEQ ID NO:231 is a 27-nucleotide sequence corresponding to an OgRRS 3F-5.

[0080] SEQ ID NO:232 is a 27-nucleotide sequence corresponding to an OgRRS 3F-9.

[0081] SEQ ID NO:233 is a 21-nucleotide sequence for the gRNA repeat for LbCas12a.

[0082] SEQ ID NO:234 is a 21-nucleotide sequence for the gRNA repeat for FnCas12a.

[0083] SEQ ID NO:235 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_5F-41.

[0084] SEQ ID NO:236 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_5F-43.

[0085] SEQ ID NO:237 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_5J-1.

[0086] SEQ ID NO:238 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_TI532.

[0087] SEQ ID NO:239 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_TI-1025.

[0088] SEQ ID NO:240 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_TI-1676.

[0089] SEQ ID NO:241 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_3J-1.

[0090] SEQ ID NO:242 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_3F-5.

[0091] SEQ ID NO:243 is a 51-nucleotide sequence corresponding to a gRNA, gRNA_3F-9.21 US_ACTIVE\129309744\V-1DETAILED DESCRIPTION

[0092] The present disclosure provides modifications of transgenic corn event MON87429. Transgenic corn event MON87429 confers tolerance to inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group, synthetic auxins, inhibitors of glutamine synthetase, and inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), or any combination thereof by expression of FT_T protein (FT), dicamba monooxygenase (DMO), phosphinothricin N-acetyltransferase (PAT), and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Reference to corn event MON87429 herein is intended as being equivalent to a reference to MON 87429, event MON87429, event MON 87429, MON87429 event, and MON 87429 event; the references are interchangeable.

[0093] The herbicide tolerance provided by event MON87429 results from the expression of a DNA segment encoding four herbicide tolerance proteins, that are operably and covalently linked within the inserted transgenic DNA that in part defines the corn event MON87429. The four proteins in the MON87429 event are a FT_T protein (FT), dicamba monooxygenase (DMO), phosphinothricin N-acetyltransferase (PAT) and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). These four herbicide tolerance proteins are expressed from the four expression cassettes within the inserted transgenic DNA as set forth in SEQ ID NO: 9 and illustrated in Fig. 1.

[0094] The PAT expression cassette in corn event MON87429 comprises in operable linkage a ubiquitin promoter, leader, and intron from Erianthus ravennae, a phosphinothricin N- acetyltransferase coding sequence, and a fructose-bisphosphate aldolase 3' UTR from Setaria italica. The DMO expression cassette in corn event MON87429 comprises in operable linkage a ubiquitin promoter, leader, and intron from Coix lacryma-jobi, an albino and pale green 6 chloroplast transit peptide coding sequence from Arabidopsis thaliana, a dicamba monooxygenase coding sequence, and a metallothionein-like protein 3’ UTR from Oryza sativa. The FT expression cassette in corn event MON87429 comprises in operable linkage a ubiquitin promoter, leader, and intron from Arundo donax, a malate dehydrogenase chloroplast transit peptide coding sequence from Arabidopsis thaliana, a FT_T protein coding sequence, and a no apical meristem protein 3’ UTR from Oryza sativa. The EPSPS expression cassette in corn event MON87429 comprises in operable linkage a CaMV 35S promoter and leader, a chlorophyll a / b-binding protein leader from Triticum aestivum, an actin 1 intron from Oryza sativa, a ShkG chloroplast transit peptide coding22 US_ACTIVE\129309744\V-1sequence from Arabidopsis thaliana, a glyphosate tolerant 5-enolpyruvylshikimate-3-phosphate synthase coding sequence from Agrobacterium sp strain CP4, a male tissue specific siRNA target from Zea mays, and a glycine-rich RNA binding protein 3’UTR from Oryza sativa. The relative position of the four expression cassettes in SEQ ID NO:9 (transgenic insert) and SEQ ID NO:10 (MON87429 event DNA) is shown in Fig. 1.

[0095] As described herein, numerous constructs which varied in the use of expression elements and combinations, transgenes coding sequences and orientation were evaluated. The construct used to create corn event MON87429 provided superior performance relative to other constructs when evaluated for herbicide tolerance.

[0096] The event MON87429 was selected based on comparisons to thousands of different independent transgenic events. The events generated expressing the herbicide tolerance genes were compared among themselves and to non-transgenic corn control (wild-type or WT) plants of the same variety. The results as illustrated in the Examples show that event MON87429 displayed superior properties due to expression of the PAT, DMO, FT and EPSPS proteins.

[0097] MON87429 was created through plant transformation techniques used to insert heterologous DNA (also known as transgenic DNA) randomly into a chromosome of the genome of a corn cell to produce a genetically engineered corn cell, also referred to as a “transgenic” or “recombinant” corn cell. Using this technique, many individual cells are transformed, each resulting in a unique “transgenic event” or “event” due to the random insertion of the foreign DNA into the genome. A transgenic plant is then regenerated from each individual transgenic cell. This results in every cell of the transgenic plant containing the uniquely inserted transgenic event as a stable part of its genome. This transgenic plant can then be used to produce seed which are then planted and grown into progeny plants, each containing the unique transgenic event.

[0098] As described herein, corn event MON87429 was produced by a complex research and development process in which: (1) 35 different transformation constructs were developed after testing various individual expression cassettes with different expression element combinations and transgenes – which included the coding sequences for the four herbicide tolerance genes, different combinations of the transcriptional regulatory elements, and relative position and orientation of the four cassettes within the constructs – and transformed through Agrobacterium-mediated transformation into corn cells of LH244 immature embryos to create 15,326 unique23 US_ACTIVE\129309744\V-1transformation, each made by a random insertion of the transgene insert into the corn genome; (2) the population of transgenic plants in which each plant contained a unique transgenic event was regenerated and tested; (3) the final event MON87429 was selected after a rigorous multi-year event selection process involving the testing and analysis of molecular characteristics, herbicide tolerance efficacy, mRNA and protein expression, and agronomic properties in a variety of genetic backgrounds. Corn event MON87429 was thus produced and selected as a uniquely superior event useful for broad-scale agronomic purposes.

[0099] The plasmid DNA inserted into the genome of corn event MON87429 was characterized by detailed molecular analysis. This analysis included: the insert number (number of integration sites within the corn genome), the genomic insert location (the specific site in the corn genome where the insertion occurred), the copy number (the number of copies of the T-DNA within one locus), presence / absence of the plasmid DNA backbone, and the integrity of the transgenic inserted DNA. The detailed molecular analysis demonstrated that the integrated T-DNA containing the PAT, DMO, FT and EPSPS expression cassettes remained intact after integration. As used herein, an “expression cassette” or “cassette” is a recombinant DNA molecule comprising a combination of distinct elements that are to be expressed by a transformed cell. Table 1 provides a list of the elements contained in SEQ ID NO:10, the DNA sequence that corresponds to corn event MON87429. Table 1. Description of corn event MON87429 Positi Description Element on in SE ID NO 1024 US_ACTIVE\129309744\V-1Posit Description Element ion in SEQ ID NO:1025 US_ACTIVE\129309744\V-1Position in Description Element SEQ ID NO:10

[0000] Corn even O 8 9 s c arac er zed as an nser on n o a s ng e ocus n e corn genome, resulting in two new loci or junction sequences (e.g., sequences set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ IDNO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, and SEQ ID NO:8) between the inserted DNA and the corn genome DNA that are not known to appear naturally in the corn genome or other transgenic corn events – they are unique to event MON87429. These junction sequences are useful in detecting the presence of the event MON87429 in corn cells, corn tissue, corn seed, and corn plants or corn plant products, such as corn commodity products. DNA molecular probes and primer pairs are described herein that have been developed for use in identifying the presence of these various junction segments in biological samples containing or suspected of containing corn cells, corn seed, corn plant parts, or corn plant tissue that contain the event MON87429.

[0100] As used herein, a “corn event MON87429 locus” refers to the genomic locus of the corn event MON87429 or a modified corn event MON87429 or a further modified corn event MON87429 including the flanking, junction and insertion sequences of the corn event MON87429 or the modified corn event MON87429 or further modified corn event MON87429. A modified corn event MON87429 or a further modified corn event MON87429 comprises one or more mutations, edits and / or genetic modifications such as insertions, deletions, or genetic element replacements in the corn event MON87429 locus relative to the corn event MON87429.26 US_ACTIVE\129309744\V-1

[0101] A modified corn event MON87429 and methods of making a modified corn event MON87429 are provided. As is described further herein, various mutagenesis or targeted genome editing techniques and related tools exist or could be made or engineered to permit genetic modification or mutation of the transgenic insert, junction and / or the flanking genomic DNA of corn event MON87429, such as by deletion, insertion, transposition, inversion, and / or substitution of nucleic acid sequence(s), or by insertion or introduction of a guide RNA target site or a cognate target site or CgRRS, and the transgenic event as modified may still be uniquely characterized by the presence of heterologous DNA and / or one or more sequences of the insertion, junction(s) and / or flanking sequence described herein at the particular position in the genome previously occupied by the unmodified corn event MON87429 relative to flanking portions or sequences of the native corn genome. A modified transgenic event derived from corn event MON87429 may comprise all or part of the insertion sequence and / or transgene cassette of corn event MON87429 and / or one or more flanking sequences described herein. As used herein, a “modified corn event MON87429” refers to the genomic DNA or sequence of the corn event MON87429 locus comprising one or more mutations, edits, or genetic modifications relative to the genomic DNA or sequence of the corn event MON87429, wherein such mutations or edits are introduced or made by a mutagenesis or targeted genome editing technique of a corn plant, plant part, tissue or cell comprising the corn event MON87429. A “modified corn event MON87429” includes a “further modified corn event MON87429” made by first inserting a target site or cognate target site or CgRRS into the corn event MON87429 locus and then further modifying the corn event MON87429 locus as described herein. Methods and techniques of mutagenesis are known in the art and include, for example, chemical mutagenesis (i.e., treatment with a chemical mutagen, such as an azide, hydroxylamine, nitrous acid, acridine, nucleotide base analog, or alkylating agent – e.g., EMS (ethylmethane sulfonate), MNU (N-methyl-N-nitrosourea), etc.), physical mutagenesis (e.g., gamma rays, X-rays, UV, ion beam, other forms of radiation, etc.), and insertional mutagenesis (e.g., transposon or T-DNA insertion). As used herein, a “modified corn plant” refers to a corn or maize plant comprising a modified corn event MON87429 or a further modified corn event MON87429, and thus a modified corn plant part, plant seed, plant tissue, or plant cell is derived from a modified corn plant and / or comprises a modified corn event MON87429.

[0102] As used herein, a “target site” for genome editing refers to the location of a polynucleotide sequence within a plant genome, which may be within or near corn event MON87429, that is27 US_ACTIVE\129309744\V-1bound and cleaved by a site-specific nuclease introducing a double stranded break (or single- stranded nick) into the nucleic acid backbone of the polynucleotide sequence and / or its complementary DNA strand. A target site may comprise at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 29, or at least 30 consecutive nucleotides. A “target site” for an RNA-guided nuclease may comprise the sequence of either complementary strand of a double-stranded nucleic acid (DNA) molecule or chromosome at the target site that is bound or hybridized to a guide RNA of a ribonucleoprotein complex comprising the RNA-guided nuclease. A site-specific nuclease may bind to a target site, such as via a non-coding guide RNA (e.g., without being limiting, a CRISPR RNA (crRNA) or a single- guide RNA (sgRNA) as described further below). A non-coding guide RNA provided herein may be complementary to a target site (e.g., complementary to either strand of a double-stranded nucleic acid molecule or chromosome at the target site). It will be appreciated that perfect identity or complementarity may not be required for a non-coding guide RNA to bind or hybridize to a target site. For example, at least 1, at least 2, at least 3, at least 4, at least 5, at least 6, at least 7, or at least 8 mismatches (or more) between a target site and a non-coding RNA may be tolerated. A “target site” also refers to the location of a polynucleotide sequence within a plant genome, which may be within or near corn event MON87429, that is bound and cleaved by another site- specific nuclease that may not be guided by a non-coding RNA molecule, such as a meganuclease, zinc finger nuclease (ZFN), or a transcription activator-like effector nuclease (TALEN), to introduce a double stranded break (or single-stranded nick) into the polynucleotide sequence and / or its complementary DNA strand. As used herein, a “target region” or a “targeted region” refers to a polynucleotide sequence or region that is flanked by two or more target sites. Without being limiting, in some embodiments a target region may be subjected to a mutation, deletion, insertion, or inversion. As used herein, “flanked” when used to describe a target region of a polynucleotide sequence or molecule, refers to two or more target sites of the polynucleotide sequence or molecule surrounding the target region, with one target site on each side of the target region.

[0103] As used herein, a “donor molecule”, “donor template”, or “donor template molecule” (collectively a “donor template”), which may be a recombinant DNA donor template, is defined as a nucleic acid molecule having a nucleic acid template or insertion sequence for site-directed,28 US_ACTIVE\129309744\V-1targeted insertion or recombination into the genome of a plant cell via repair of a nick or double- stranded DNA break in the genome of a plant cell. For example, a “donor template” may be used for site-directed integration of a guide RNA target site or a cognate target site or CgRRS into a target site within the genome of a plant. A targeted genome editing technique provided herein may comprise the use of one or more, two or more, three or more, four or more, or five or more donor molecules or templates. A “donor template” may be a single-stranded or double-stranded DNA or RNA molecule or plasmid. An “insertion sequence” of a donor template is a sequence designed for targeted insertion into the genome of a plant cell, which may be of any suitable length, such as to include a guide RNA target site or a cognate target site or CgRRS. Such an insertion sequence of a donor template is distinct and different from the transgenic insertion or insert of corn event MON87429, although they may potentially have sequence(s) in common. A donor template may also have at least one homology sequence or homology arm, such as two homology arms, to direct the integration of a mutation or insertion sequence into a target site within the genome of a plant via homologous recombination, wherein the homology sequence or homology arm(s) are identical or complementary, or have a percent identity or percent complementarity, to a sequence at or near the target site within the genome of the plant. When a donor template comprises homology arm(s) and an insertion sequence, the homology arm(s) will flank or surround the insertion sequence of the donor template.

[0104] As used herein, a “targeted genome editing technique” refers to any method, protocol, or technique that can be used to make a targeted mutation or edit, such as one or more insertions, deletions, substitutions, inversions, transpositions, mutations and / or other genetic modifications at or near a target site in the genome of a plant, and / or a deletion or excision of a target region between two target sites in the genome of a plant, using a site-specific nuclease, such as a meganuclease, a zinc-finger nuclease (ZFN), an RNA-guided endonuclease (e.g., the CRISPR / Cas9 system), a TALE-endonuclease (TALEN), a recombinase, or a transposase. A site-specific nuclease may introduce a double stranded break (or single-stranded nick) into the nucleic acid backbone of the polynucleotide sequence and / or its complementary DNA strand. Following the introduction of the single or double-stranded break or nick or cleavage of DNA at or near the target site(s) by the site- specific nuclease, the genomic sequence can be repaired via a double strand break repair pathway, which may include, for example, non-homologous end-joining (NHEJ), microhomology-mediated end joining (MMEJ), homologous recombination, synthesis-dependent strand annealing (SDSA),29 US_ACTIVE\129309744\V-1single-strand annealing (SSA), or a combination of any thereof, at or near the target site(s). However, if the repair is imperfect, a mutation or edit may be introduced at or near the target site(s), and a target region between two or more target sites may be deleted or excised.

[0105] A “site-specific nuclease” provided herein may be selected from the group consisting of a zinc-finger nuclease (ZFN), a meganuclease, an RNA-guided endonuclease, a TALE- endonuclease (TALEN), a recombinase, a transposase, or any combination thereof. See, e.g., Khandagale, K. et al., “Genome editing for targeted improvement in plants,” Plant Biotechnol Rep 10: 327-343 (2016); and Gaj, T. et al., “ZFN, TALEN and CRISPR / Cas-based methods for genome engineering,” Trends Biotechnol. 31(7): 397-405 (2013), the contents and disclosures of which are incorporated herein by reference. A recombinase may be a serine recombinase attached to a DNA recognition motif, a tyrosine recombinase attached to a DNA recognition motif or other recombinase enzyme known in the art. A recombinase or transposase may be a DNA transposase or recombinase attached to a DNA binding domain. A tyrosine recombinase attached to a DNA recognition motif may be selected from the group consisting of a Cre recombinase, a Flp recombinase, and a Tnp1 recombinase. A Cre recombinase or a Gin recombinase provided herein is tethered to a zinc-finger DNA binding domain. A serine recombinase attached to a DNA recognition motif provided herein is selected from the group consisting of a PhiC31 integrase, an R4 integrase, and a TP-901 integrase. A DNA transposase attached to a DNA binding domain provided herein is selected from the group consisting of a TALE-piggyBac and TALE-Mutator.

[0106] An RNA-guided endonuclease may be selected from the group consisting of Cas1, Cas1B, Cas2, Cas3, Cas4, Cas5, Cas6, Cas7, Cas8, Cas9 (also known as Csn1 and Csx12), Cas10, Csy1, Csy2, Csy3, Cse1, Cse2, Csc1, Csc2, Csa5, Csn2, Csm2, Csm3, Csm4, Csm5, Csm6, Cmr1, Cmr3, Cmr4, Cmr5, Cmr6, Csb1, Csb2, Csb3, Csx17, Csx14, Csx10, Csx16, CsaX, Csx3, Csx1, Csx15, Csf1, Csf2, Csf3, Csf4, Cas12a, Cpf1, CasX, CasY, and homologs or modified versions thereof, Argonaute (non-limiting examples of Argonaute proteins include Thermus thermophilus Argonaute (TtAgo), Pyrococcus furiosus Argonaute (PfAgo), Natronobacterium gregoryi Argonaute (NgAgo) and homologs or modified versions thereof. According to some embodiments, an RNA-guided endonuclease may be a Cas9 or Cas12a or Cpf1 enzyme.

[0107] A site-specific nuclease provided herein is selected from the group consisting of a zinc- finger nuclease, a meganuclease, an RNA-guided nuclease, a TALE-nuclease, a recombinase, a30 US_ACTIVE\129309744\V-1transposase, or any combination thereof. In another aspect, a site-specific nuclease provided herein is selected from the group consisting of a Cas9 or a Cas12a or Cpf1. In another aspect, a site-specific nuclease provided herein is selected from the group consisting of a Cas1, a Cas1B, a Cas2, a Cas3, a Cas4, a Cas5, a Cas6, a Cas7, a Cas8, a Cas9, a Cas10, a Cas12a, a Csy1, a Csy2, a Csy3, a Cse1, a Cse2, a Csc1, a Csc2, a Csa5, a Csn2, a Csm2, a Csm3, a Csm4, a Csm5, a Csm6, a Cmr1, a Cmr3, a Cmr4, a Cmr5, a Cmr6, a Csb1, a Csb2, a Csb3, a Csx17, a Csx14, a Csx10, a Csx16, a CsaX, a Csx3, a Csx1, a Csx15, a Csf1, a Csf2, a Csf3, a Csf4, a Cpf1, CasX, CasY, a homolog thereof, or a modified version thereof. In another aspect, an RNA-guided nuclease provided herein is selected from the group consisting of a Cas9 or a Cas12a or Cpf1. In another aspect, an RNA guided nuclease provided herein is selected from the group consisting of a Cas1, a Cas1B, a Cas2, a Cas3, a Cas4, a Cas5, a Cas6, a Cas7, a Cas8, a Cas9, a Cas10, a Cas12a, a Csy1, a Csy2, a Csy3, a Cse1, a Cse2, a Csc1, a Csc2, a Csa5, a Csn2, a Csm2, a Csm3, a Csm4, a Csm5, a Csm6, a Cmr1, a Cmr3, a Cmr4, a Cmr5, a Cmr6, a Csb1, a Csb2, a Csb3, a Csx17, a Csx14, a Csx10, a Csx16, a CsaX, a Csx3, a Csx1, a Csx15, a Csf1, a Csf2, a Csf3, a Csf4, a Cpf1, CasX, CasY, a homolog thereof, or a modified version thereof. In another aspect, a method and / or a composition provided herein comprises at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten site-specific nucleases. In yet another aspect, a method and / or a composition provided herein comprises at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten polynucleotides encoding at least one, at least two, at least three, at least four, at least five, at least six, at least seven, at least eight, at least nine, or at least ten site- specific nucleases.

[0108] For RNA-guided endonucleases, a guide RNA (gRNA) molecule is further provided to direct the endonuclease to a target site in the genome of the plant via base-pairing or hybridization to cause a DSB or nick at or near the target site. The gRNA may be transformed or introduced into a plant cell or tissue (perhaps along with a nuclease, or nuclease-encoding DNA molecule, construct or vector) as a gRNA molecule, or as a recombinant DNA molecule, construct or vector comprising a polynucleotide or transcribable DNA sequence encoding the guide RNA operably linked to a plant-expressible promoter. As understood in the art, a “guide RNA” may comprise, for example, a CRISPR RNA (crRNA), a single-chain guide RNA (sgRNA), or any other RNA molecule that may guide or direct an endonuclease to a specific target site in the genome. A31 US_ACTIVE\129309744\V-1“single-chain guide RNA” (or “sgRNA”) is an RNA molecule comprising a crRNA covalently linked a tracrRNA by a linker sequence, which may be expressed as a single RNA transcript or molecule. The guide RNA comprises a guide or targeting sequence that is identical or complementary to a target site within the plant genome, such as within or near corn event MON87429. A protospacer-adjacent motif (PAM) may be present in the genome immediately adjacent and upstream or downstream of the genomic target site sequence complementary to the targeting sequence of the guide RNA as known in the art. See, e.g., Wu, X. et al., “Target specificity of the CRISPR-Cas9 system,” Quant Biol. 2(2): 59-70 (2014), the content and disclosure of which is incorporated herein by reference. The guide RNA may typically be a non- coding RNA molecule that does not encode a protein. The guide sequence of the guide RNA may be at least 10 nucleotides in length, such as 12-40 nucleotides, 12-30 nucleotides, 12-20 nucleotides, 12-35 nucleotides, 12-30 nucleotides, 15-30 nucleotides, 17-30 nucleotides, or 17-25 nucleotides in length, or about 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25 or more nucleotides in length. The guide sequence may be at least 95%, at least 96%, at least 97%, at least 99% or 100% identical or complementary to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, or more consecutive nucleotides of a DNA sequence at the genomic target site. As used herein, the term “consecutive” in reference to a polynucleotide or protein sequence means without deletions or gaps in the sequence.

[0109] In addition to the guide sequence, a guide RNA may further comprise one or more other structural or scaffold sequence(s), which may bind or interact with an RNA-guided endonuclease. Such scaffold or structural sequences may further interact with other RNA molecules (e.g., tracrRNA). Methods and techniques for designing targeting constructs and guide RNAs for genome editing and site-directed integration at a target site within the genome of a plant using an RNA-guided endonuclease are known in the art.

[0110] Recombinant DNA molecules, constructs and vectors are provided comprising a polynucleotide or transcribable DNA sequence encoding a site-specific nuclease, such as a zinc- finger nuclease (ZFN), a meganuclease, an RNA-guided endonuclease, a TALE-endonuclease (TALEN), a recombinase, or a transposase, wherein the coding sequence is operably linked to a plant expressible promoter. For RNA-guided endonucleases, recombinant DNA molecules, constructs and vectors are further provided comprising a polynucleotide or transcribable DNA32 US_ACTIVE\129309744\V-1sequence encoding a guide RNA, wherein the guide RNA comprises a guide sequence of sufficient length having a percent identity or complementarity to a target site within the genome of a plant. Recombinant DNA molecules, constructs and vectors are provided comprising a first polynucleotide or transcribable DNA sequence encoding a site-specific nuclease and a second polynucleotide or transcribable DNA sequence encoding one or more gRNAs. In some aspects, each polynucleotide or transcribable DNA sequence of a recombinant DNA molecule, construct and vector that encodes a site-specific nuclease and / or a guide RNA may be operably linked to a plant expressible promoter, such as an inducible promoter, a constitutive promoter, a tissue- specific promoter, etc.

[0111] Recombinant DNA molecules, constructs and vectors are provided comprising a polynucleotide or transcribable DNA sequence encoding a site-specific nuclease, such as a zinc- finger nuclease (ZFN), a meganuclease, an RNA-guided endonuclease, a TALE-endonuclease (TALEN), a recombinase, or a transposase, wherein the coding sequence is operably linked to a plant expressible promoter. For RNA-guided endonucleases, recombinant DNA molecules, constructs and vectors are further provided comprising a polynucleotide or transcribable DNA sequence encoding a guide RNA, wherein the guide RNA comprises a guide sequence of sufficient length having a percent identity or complementarity to a target site within the genome of a plant. In some aspects, recombinant DNA molecules, constructs and vectors are provided comprising a first polynucleotide or transcribable DNA sequence encoding a site-specific nuclease and a second polynucleotide or transcribable DNA sequence encoding one or more gRNAs. In some aspects, each polynucleotide or transcribable DNA sequence of a recombinant DNA molecule, construct and vector that encodes a site-specific nuclease and / or a guide RNA may be operably linked to a plant expressible promoter, such as an inducible promoter, a constitutive promoter, a tissue- specific promoter, etc.

[0112] A recombinant DNA molecule, construct or vector may comprise a first polynucleotide sequence encoding a site-specific nuclease and a second polynucleotide sequence encoding a guide RNA(s) that may be introduced into a plant cell together via plant transformation techniques. Alternatively, two recombinant DNA molecules, constructs or vectors may be provided including a first recombinant DNA molecule, construct or vector and a second DNA molecule, construct or vector that may be introduced into a plant cell together or sequentially via plant transformation techniques, wherein the first recombinant DNA molecule, construct or vector comprises a33 US_ACTIVE\129309744\V-1polynucleotide sequence encoding a site-specific nuclease and the second recombinant DNA molecule, construct or vector comprises a polynucleotide sequence encoding a guide RNA(s). In some aspects, a recombinant DNA molecule, construct or vector comprising a polynucleotide sequence encoding a site-specific nuclease may be introduced via plant transformation techniques into a plant cell that already comprises (or is transformed with) a recombinant DNA construct or vector comprising a polynucleotide sequence encoding a guide RNA(s). Alternatively, a recombinant DNA molecule, construct or vector comprising a polynucleotide sequence encoding a guide RNA may be introduced via plant transformation techniques into a plant cell that already comprises (or is transformed with) a recombinant DNA construct or vector comprising a polynucleotide sequence encoding a site-specific nuclease. In some aspects, a first plant comprising (or transformed with) a recombinant DNA construct or vector comprising a polynucleotide sequence encoding a site-specific nuclease may be crossed with a second plant comprising (or transformed with) a recombinant DNA construct or vector comprising a polynucleotide sequence encoding a guide RNA(s). Such recombinant DNA molecules, constructs or vectors may be transiently transformed into a plant cell or stably transformed or more preferably integrated into the genome of a plant cell.

[0113] Molecules or vectors comprising polynucleotides encoding a site-specific nuclease, and optionally one or more, two or more, three or more, or four or more gRNAs are provided to a plant cell by transformation methods known in the art (e.g., without being limiting, particle bombardment, PEG-mediated protoplast transfection or Agrobacterium-mediated transformation). In an aspect, molecules or vectors comprising polynucleotides encoding a Cas9 nuclease, and optionally one or more, two or more, three or more, or four or more gRNAs are provided to a plant cell by transformation methods known in the art (e.g., without being limiting, particle bombardment, PEG-mediated protoplast transfection or Agrobacterium-mediated transformation). In another aspect, vectors comprising polynucleotides encoding a Cpf1 and, optionally one or more, two or more, three or more, or four or more crRNAs are provided to a cell by transformation methods known in the art (e.g., without being limiting, viral transfection, particle bombardment, PEG-mediated protoplast transfection or Agrobacterium-mediated transformation).

[0114] Several site-specific nucleases, such as recombinases, zinc finger nucleases (ZFNs), meganucleases, and TALENs, are not RNA-guided and instead rely on their protein structure to determine their target site for causing the DSB or nick, or they are fused, tethered or attached to a34 US_ACTIVE\129309744\V-1DNA-binding protein domain or motif. The protein structure of the site-specific nuclease (or the fused / attached / tethered DNA binding domain) may target the site-specific nuclease to the target site. According to many of these embodiments, non-RNA-guided site-specific nucleases, such as recombinases, zinc finger nucleases (ZFNs), meganucleases, and TALENs, may be designed, engineered and constructed according to known methods to target and bind to a target site in the genome of a plant, to create a DSB or nick at or near such genomic target site or locus. For example, an engineered site-specific nuclease, such as a recombinase, zinc finger nuclease (ZFN), meganuclease, or TALEN, may be designed to target and bind to a genomic target site within the genome of a plant to create a DSB or nick at the genomic target site.

[0115] A targeted genome editing technique described herein may comprise the use of a zinc finger nuclease (ZFN). ZFNs are synthetic proteins consisting of an engineered zinc finger DNA-binding domain fused to a cleavage domain (or a cleavage half-domain), which may be derived from a restriction endonuclease (e.g., FokI). The DNA binding domain may be canonical (C2H2) or non- canonical (e.g., C3H or C4). The DNA-binding domain can comprise one or more zinc fingers (e.g., 2, 3, 4, 5, 6, 7, 8, 9 or more zinc fingers) depending on the target site. Multiple zinc fingers in a DNA-binding domain may be separated by linker sequence(s). ZFNs can be designed to cleave almost any stretch of double-stranded DNA by modification of the zinc finger DNA-binding domain. ZFNs form dimers from monomers composed of a non-specific DNA cleavage domain (e.g., derived from the FokI nuclease) fused to a DNA-binding domain comprising a zinc finger array engineered to bind a target site DNA sequence. The DNA-binding domain of a ZFN may typically be composed of 3-4 (or more) zinc-fingers. The amino acids at positions -1, +2, +3, and +6 relative to the start of the zinc finger α-helix, which contribute to site-specific binding to the target site, can be changed and customized to fit specific target sequences. The other amino acids may form a consensus backbone to generate ZFNs with different sequence specificities. Methods and rules for designing ZFNs for targeting and binding to specific target sequences are known in the art. See, e.g., US Patent App. Nos. 2005 / 0064474, 2009 / 0117617, and 2012 / 0142062, the contents and disclosures of which are incorporated herein by reference. The FokI nuclease domain may require dimerization to cleave DNA and therefore two ZFNs with their C-terminal regions are needed to bind opposite DNA strands of the cleavage site (separated by 5-7 bp). The ZFN monomer can cut the target site if the two-ZF-binding sites are palindromic. A ZFN, as used herein, is broad and includes a monomeric ZFN that can cleave double stranded DNA without35 US_ACTIVE\129309744\V-1assistance from another ZFN. The term ZFN may also be used to refer to one or both members of a pair of ZFNs that are engineered to work together to cleave DNA at the same site.

[0116] Without being limited by any scientific theory, because the DNA-binding specificities of zinc finger domains can be re-engineered using one of various methods, customized ZFNs can theoretically be constructed to target nearly any target sequence (e.g., at or near corn event MON87429 in a plant genome). Publicly available methods for engineering zinc finger domains include Context-dependent Assembly (CoDA), Oligomerized Pool Engineering (OPEN), and Modular Assembly. In an aspect, a method and / or composition provided herein comprises one or more, two or more, three or more, four or more, or five or more ZFNs. In another aspect, a ZFN provided herein is capable of generating a targeted DSB or nick. In an aspect, vectors comprising polynucleotides encoding one or more, two or more, three or more, four or more, or five or more ZFNs are provided to a cell by transformation methods known in the art (e.g., without being limiting, viral transfection, particle bombardment, PEG-mediated protoplast transfection, or Agrobacterium-mediated transformation). The ZFNs may be introduced as ZFN proteins, as polynucleotides encoding ZFN proteins, and / or as combinations of proteins and protein-encoding polynucleotides.

[0117] A targeted genome editing technique described herein may comprise the use of a meganuclease. Meganucleases, which are commonly identified in microbes, such as the LAGLIDADG family of homing endonucleases, are unique enzymes with high activity and long recognition sequences (> 14 bp) resulting in site-specific digestion of target DNA. Engineered versions of naturally occurring meganucleases typically have extended DNA recognition sequences (for example, 14 to 40 bp). According to some aspects, a meganuclease may comprise a scaffold or base enzyme selected from the group consisting of I-CreI, I-CeuI, I-MsoI, I-SceI, I- AniI, and I-DmoI. The engineering of meganucleases can be more challenging than ZFNs and TALENs because the DNA recognition and cleavage functions of meganucleases are intertwined in a single domain. Specialized methods of mutagenesis and high-throughput screening have been used to create novel meganuclease variants that recognize unique sequences and possess improved nuclease activity. Thus, a meganuclease may be selected or engineered to bind to a genomic target site or sequence in a plant. In an aspect, a method and / or composition provided herein comprises one or more, two or more, three or more, four or more, or five or more meganucleases. In another aspect, a meganuclease provided herein is capable of generating a targeted DSB. In an aspect,36 US_ACTIVE\129309744\V-1vectors comprising polynucleotides encoding one or more, two or more, three or more, four or more, or five or more meganucleases are provided to a cell by transformation methods known in the art (e.g., without being limiting, viral transfection, particle bombardment, PEG-mediated protoplast transfection or Agrobacterium-mediated transformation).

[0118] A targeted genome editing technique described herein may comprise the use of a transcription activator-like effector nuclease (TALEN). TALENs are artificial restriction enzymes generated by fusing the transcription activator-like effector (TALE) DNA binding domain to a nuclease domain (e.g., FokI). In some aspects, the nuclease is selected from a group consisting of PvuII, MutH, TevI, FokI, AlwI, MlyI, SbfI, SdaI, StsI, CleDORF, Clo051, and Pept071. For FokI nuclease, when each member of a TALEN pair binds to the DNA sites flanking a target site, the FokI monomers dimerize and cause a double-stranded DNA break at the target site. Besides the wild-type FokI cleavage domain, variants of the FokI cleavage domain with mutations have been designed to improve cleavage specificity and cleavage activity. The FokI domain functions as a dimer, requiring two constructs with unique DNA binding domains for sites in the target genome with proper orientation and spacing. Both the number of amino acid residues between the TALEN DNA binding domain and the FokI cleavage domain and the number of bases between the two individual TALEN binding sites are parameters for achieving high levels of activity. The term TALEN, as used herein, is broad and includes a monomeric TALEN that can cleave double stranded DNA without assistance from another TALEN. The term TALEN also refers to one or both members of a pair of TALENs that work together to cleave DNA at the same site.

[0119] Besides the wild-type FokI cleavage domain, variants of the FokI cleavage domain with mutations have been designed to improve cleavage specificity and cleavage activity. The FokI domain functions as a dimer, requiring two constructs with unique DNA binding domains for sites in the target genome with proper orientation and spacing. Both the number of amino acid residues between the TALEN DNA binding domain and the FokI cleavage domain and the number of bases between the two individual TALEN binding sites are parameters for achieving high levels of activity. PvuII, MutH, and TevI cleavage domains are useful alternatives to FokI and FokI variants for use with TALEs. PvuII functions as a highly specific cleavage domain when coupled to a TALE (see Yank et al. 2013. PLoS One. 8: e82539). MutH is capable of introducing strand- specific nicks in DNA (see Gabsalilow et al. 2013. Nucleic Acids Research. 41: e83). TevI37 US_ACTIVE\129309744\V-1introduces double-stranded breaks in DNA at targeted sites (see Beurdeley et al., 2013. Nature Communications. 4: 1762).

[0120] Transcription activator-like effectors (TALEs) can be engineered to bind practically any DNA sequence, such as at or near the genomic locus of corn event MON87429 in a plant. TALE has a central DNA-binding domain composed of 13-28 repeat monomers of 33-34 amino acids. The amino acids of each monomer are highly conserved, except for hypervariable amino acid residues at positions 12 and 13. The two variable amino acids are called repeat-variable diresidues (RVDs). The amino acid pairs NI, NG, HD, and NN of RVDs preferentially recognize adenine, thymine, cytosine, and guanine / adenine, respectively, and modulation of RVDs can recognize consecutive DNA bases. This simple relationship between amino acid sequence and DNA recognition has allowed for the engineering of specific DNA binding domains by selecting a combination of repeat segments containing the appropriate RVDs. The relationship between amino acid sequence and DNA recognition of the TALE binding domain allows for designable proteins. Software programs such as DNA Works can be used to design TALE constructs. Other methods of designing TALE constructs are known to those of skill in the art. See Doyle et al., Nucleic Acids Research (2012) 40: W117-122.; Cermak et al., Nucleic Acids Research (2011). 39:e82; and tale-nt.cac.cornell.edu / about. In an aspect, a method and / or composition provided herein comprises one or more, two or more, three or more, four or more, or five or more TALENs. In another aspect, a TALEN provided herein is capable of generating a targeted DSB. In an aspect, vectors comprising polynucleotides encoding one or more, two or more, three or more, four or more, or five or more TALENs are provided to a cell by transformation methods known in the art (e.g., without being limiting, viral transfection, particle bombardment, PEG-mediated protoplast transfection or Agrobacterium-mediated transformation). See, e.g., US Patent App. Nos. 2011 / 0145940, 2011 / 0301073, and 2013 / 0117869, the contents and disclosures of which are incorporated herein by reference.

[0121] A targeted genome editing technique described herein may comprise the use of a recombinase. In some embodiments, a tyrosine recombinase attached, etc., to a DNA recognition domain or motif may be selected from the group consisting of a Cre recombinase, a Flp recombinase, and a Tnp1 recombinase. In an aspect, a Cre recombinase or a Gin recombinase provided herein may be tethered to a zinc-finger DNA binding domain. The Flp-FRT site-directed recombination system may come from the 2µ plasmid from the baker’s yeast Saccharomyces38 US_ACTIVE\129309744\V-1cerevisiae. In this system, Flp recombinase (flippase) may recombine sequences between flippase recognition target (FRT) sites. FRT sites comprise 34 nucleotides. Flp may bind to the “arms” of the FRT sites (one arm is in reverse orientation) and cleaves the FRT site at either end of an intervening nucleic acid sequence. After cleavage, Flp may recombine nucleic acid sequences between two FRT sites. Cre-lox is a site-directed recombination system derived from the bacteriophage P1 that is similar to the Flp-FRT recombination system. Cre-lox can be used to invert a nucleic acid sequence, delete a nucleic acid sequence, or translocate a nucleic acid sequence. In this system, Cre recombinase may recombine a pair of lox nucleic acid sequences. Lox sites comprise 34 nucleotides, with the first and last 13 nucleotides (arms) being palindromic. During recombination, Cre recombinase protein binds to two lox sites on different nucleic acids and cleaves at the lox sites. The cleaved nucleic acids are spliced together (reciprocally translocated) and recombination is complete. In another aspect, a lox site provided herein is a loxP, lox 2272, loxN, lox 511, lox 5171, lox71, lox66, M2, M3, M7, or M11 site.

[0122] As used herein, the term “derived” or “derived from” in reference to a particular DNA molecule, amplicon or sequence in relation to a corn cell, corn tissue, corn seed, corn plant, corn plant part and / or corn plant product, such as a corn commodity product, means that the DNA molecule, amplicon or sequence is taken, purified, isolated, or made, directly or indirectly, from such corn cell, corn tissue, corn seed, corn plant, corn plant part and / or corn plant product, such as a corn commodity product, as the case may be. Alternatively, the term “derived” or “derived from” in reference to a corn plant product, such as a corn commodity product, in relation to a corn cell, corn tissue, corn seed, corn plant, and / or corn plant part, means that the corn plant product is taken, purified, isolated, or made, directly or indirectly, from such corn cell, corn tissue, corn seed, corn plant, and / or corn plant part, as the case may be. “Capable of being detected” refers to the ability of a particular DNA molecule, segment or sequence to be detected in a sample, such as by amplification and determining its presence, size or sequence such as by DNA sequence analysis, and / or binding of a probe to the target DNA molecule, segment or sequence.

[0123] A “sample” is intended to refer to any composition comprising or derived from, either directly or indirectly, a biological sample, source or material. The sample may generally comprise corn DNA and / or substantially or completely pure, purified or isolated corn DNA. A “biological sample” contains biological materials, including but not limited to DNA obtained or derived from, either directly or indirectly, the genome of a corn cell(s), corn tissue(s), corn seed(s), corn plant(s),39 US_ACTIVE\129309744\V-1corn plant part(s) and / or corn plant product(s), such as a corn commodity product(s). Such corn cell(s), corn tissue(s), corn seed(s), corn plant(s), corn plant part(s) and / or corn plant product(s), such as a corn commodity product(s), may comprise corn event MON87429 or DNA molecule(s) and / or DNA segment(s) comprising corn event MON87429. In some embodiments, a sample or biological sample may comprise corn cell(s), corn tissue(s), corn seed(s), corn plant(s), corn plant part(s), and / or corn plant product(s), whose cells or cellular membranes have been fractured (e.g., disrupted or opened) to release the contents of the corn cell(s) including genomic DNA and / or make the contents of the corn cell(s) including genomic DNA accessible or usable for assays or testing. “Directly” refers to directly obtaining DNA by a skilled artisan from the corn genome by fracturing corn cells (or by obtaining samples of corn that contain fractured corn cells) and exposing or using the genomic DNA from corn cells for the purposes of detection. “Indirectly” refers to obtaining by a skilled artisan a target or specific reference DNA (i.e., a novel and unique junction segment(s) described herein as being diagnostic for the presence of the event MON87429) in a particular sample, by means other than by obtaining directly via fracturing of corn cells or obtaining a sample of corn that contains fractured corn cells. Such indirect means include, but are not limited to, amplification of a DNA segment that contains a DNA sequence targeted by a particular probe(s) and / or primer set(s) designed to bind with specificity to or near the target sequence, or amplification of a DNA segment comprising all or part of a target sequence that can be measured and characterized (e.g., measured by migration or separation from other segments of DNA and / or identification in an effective matrix, such as an agarose or acrylamide gel or the like, or characterized by direct sequence analysis of the amplicon(s), or cloning of the amplicon(s) into a vector(s) and direct sequencing of the inserted amplicon(s) present within such vector(s).

[0124] Detailed molecular analysis demonstrated that event MON87429 contains a single T-DNA insertion with one copy of each of the PAT, DMO, FT and EPSPS expression cassettes. No additional elements from the transformation construct other than portions of the Agrobacterium tumefaciens left and right border regions used for transgenic DNA transfer from the plant transformation plasmid to the corn genome were identified in event MON87429. Further, thermal amplification producing specific amplicons diagnostic for the presence of event MON87429 in a sample and DNA sequence analyses were performed to determine the arbitrarily assigned 5´ and 3´ insert-to-plant genome junctions, confirm the organization of the elements within the insert, and determine the complete DNA sequence of the inserted transgenic DNA (SEQ ID NO:9). SEQ ID40 US_ACTIVE\129309744\V-1NO:14 (referred to as “5’ genomic flanking sequence” or “5’ flanking genomic sequence” or “5’ flanking sequence”) is a sequence representing the 1,000 nucleotide 5´ corn genomic DNA sequence flanking the inserted T-DNA sequence presented as SEQ ID NO:9, plus an additional 29 nucleotides at the 3’ end of the flanking genomic sequence which was inserted during T-DNA integration. SEQ ID NO:15 (referred to as “3’ genomic flanking sequence” or “3’ flanking genomic sequence” or “3’ flanking sequence”) is a sequence representing the 1000 nucleotide 3´ corn genomic DNA sequence flanking the inserted T-DNA sequence presented as SEQ ID NO:9, plus an additional 31 nucleotides at the 5’ end of the flanking genomic sequence which was also inserted during T-DNA integration. SEQ ID NO:7 is a sequence representing the 1029 nucleotide 5´ corn genomic DNA sequence flanking the inserted T-DNA sequence plus the additional 29 nucleotides (i.e., SEQ ID NO:14) combined with the 321 nucleotides of the 5’ end of the inserted T-DNA sequence presented as SEQ ID NO:9. SEQ ID NO:8 is a sequence representing 38 nucleotides of the 3’ end of the inserted T-DNA sequence with the 31 additional nucleotides inserted during T-DNA integration and the 1000 nucleotide 3´ corn genomic DNA sequence (i.e., SEQ ID NO:15). SEQ ID NO:10 corresponds to corn event MON87429 and contains a contiguous sequence (contig) comprising the 5´ flanking sequence (SEQ ID NO:14), the transgene insert of MON87429 (SEQ ID NO:9), and the 3´ flanking sequence (SEQ ID NO:15), and thus contains the insert-to-plant genome junction sequences.

[0125] Unless otherwise noted herein, terms are to be understood according to conventional usage by those of ordinary skill in the relevant art. Definitions of common terms in molecular biology may be found in Rieger et al., Glossary of Genetics: Classical and Molecular, 5thedition, Springer- Verlag: New York, 1991; and Lewin, Genes V, Oxford University Press: New York, 1994, along with other sources known to those of ordinary skill in the art. As used herein, the term “corn” means species belong to the genus Zea, preferably Zea mays and includes all plant varieties that can be bred with corn plants containing event MON87429, including wild corn species as well as those plants belonging to the genus Zea that permit breeding between species.

[0126] Provided are transgenic plants which have been transformed with a DNA construct that contains four expression cassettes to produce phosphinothricin N-acetyltransferase (PAT), dicamba monooxygenase (DMO), FT_T protein (FT), and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) and provide the transgenic plants tolerance to inhibitors of acetyl CoA41 US_ACTIVE\129309744\V-1carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group; synthetic auxins; inhibitors of glutamine synthetase; and inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).

[0127] As used herein, inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group (referred to as “FOP herbicide(s)”) include, but are not limited to, clodinafop, clodinafop-ethyl, clodinafop-propargyl, cyhalofop, cyhalofop-butyl, diclofop, diclofop-methyl, diclofop-P, diclofop-P-methyl, fenoxaprop, fenoxaprop-P, fenoxaprop-P-ethyl, fenthiaprop, fluazifop, fluazifop-butyl, fluazifop-P, fluazifop-P-butyl, fluroxypyr, haloxyfop, haloxyfop.etotyl, haloxyfop-methyl, haloxyfop-P, haloxyfop-P-methyl, isoxapyrifop, metamifop, propaquizafop, quizalofop, quizalafop-ethyl, quizalofop-P, quizalafop-P-ethyl, quizalafop-P- tefuryl, and trifop.

[0128] As used herein, synthetic auxins include, but are not limited to, benzoic acid herbicides, phenoxy acid herbicides, arylpicolinate herbicides, and pyridinyloxy acid herbicides. Examples of a benzoic acid herbicides include, but are not limited to, dicamba (3,6-dichloro-2- methoxybenzoic acid), dicamba salts, dicamba-butotyl, dicamba-diglycolamine salt, dicamba- dimethylammonium, dicamba-diethanolammonium, dicamba-isopropylammonium, dicamba- potassium, dicamba-sodium, and dicamba-trolamine. Examples of phenoxy acid herbicides are 2,4-D (2,4-dichlorophenoxyacetic acid), 2,4-D-butotyl, 2,4-D-butyl, 2,4-D-choline, 2,4-D- dimethylammonium, 2,4-D-diolamin, 2,4-D-ethyl, 2,4-D-2-ethylhexyl, 2,4-D-isobutyl, 2,4-D- isoctyl, 2,4-D-isopropyl, 2,4-D- isopropylammonium, 2,4-D-potassium, 2,4-D-sodium, 2,4-D- triisopropanolammonium, 2,4-D-trolamine, clomeprop, dichlorprop, fenoprop, MCPA, MCPA- butotyl, MCPA-dimethylammonium, MCPA-2-ethylhexyl. MCPA-isopropylammonium, MCPA- potassium, MCPA-sodium, MCPA-thioethyl, 2,4-DB, MCPB, MCPB-methyl, MCPB-ethyl- sodium, and mecoprop. Examples of arylpicolinate herbicides are halauxifen, halauxifen-methyl, and florpyrauxifen-benzyl. Examples of pyridinyloxy acid herbicides are triclopyr, fluroxypyr, aminopyralid, clopyralid, and picloram.

[0129] As used herein, inhibitors of glutamine synthetase include, but are not limited to, phosphinothricin, glufosinate, glufosinate salts, glufosinate-ammonium, glufosinate-sodium, glufosinate-P, L-glufosinate-ammonium, and L-glufosinate-sodium.

[0130] As used herein, inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) include, but are not limited to, glyphosate, glyphosate salts, glyphosate-isopropylammonium,42 US_ACTIVE\129309744\V-1glyphosate-ammonium, glyphosate-dimethylammonium, glyphosate-trimesium (=sulfosate), glyphosate-diammonium, glyphosate-potassium, and glyphosate-sodium.

[0131] As used herein, “herbicide tolerant” or “herbicide tolerance” or “tolerance” means the ability to be wholly or partially unaffected by the presence or application of one of more herbicide(s), for example to resist the toxic effects of an herbicide when applied. A cell, seed, or plant is “herbicide tolerant” or has “improved tolerance” if it can maintain at least some normal growth or phenotype in the presence of one or more herbicide(s). A trait is an herbicide tolerance trait if its presence can confer improved tolerance to an herbicide upon a cell, plant, or seed as compared to the wild-type or control cell, plant, or seed. Crops comprising an herbicide tolerance trait can continue to grow in the presence of the herbicide and may be minimally affected by the presence of the herbicide. A protein confers “herbicide tolerance” if expression of the protein can confer improved tolerance to an herbicide upon a cell, plant, or seed as compared to the wild-type or control cell, plant, or seed. Examples of herbicide tolerance proteins are phosphinothricin N- acetyltransferase, dicamba monooxygenase, the FT protein, and 5-enolpyruvylshikimate-3- phosphate synthase from Agrobacterium sp strain CP4. Herbicide tolerance may be complete or partial insensitivity to a particular herbicide and may be expressed as a percent (%) tolerance or insensitivity to a particular herbicide.

[0132] A transgenic plant is produced by transformation of a plant cell with heterologous DNA, i.e., a polynucleic acid construct that includes a number of efficacious features of interest, regeneration of a plant resulting from the insertion of the transgene into the genome of the plant cell, and selection of a particular plant characterized by insertion into a particular genome location and the number of efficacious features of the regenerated transgenic plant. The term “transgenic event” or “event” refers to the inserted transgenic DNA in the plant genome and flanking genomic sequences immediately adjacent to the inserted transgenic DNA in the genome of the transformed plant, but also refers to a DNA molecule comprising the inserted transgenic DNA in the plant genome and flanking genomic sequences. Each event is unique and would be expected to be transferred to progeny plants that receive the transgenic DNA and event through genetic inheritance and / or segregation from a parent as the result of a sexual or self-cross of a first parental line that includes the inserted transgenic DNA and event either with itself or a second parental line that may or may not contain the same transgenic DNA and event. The parental line that includes the inserted transgenic DNA and event may itself be the original transformant or a progeny plant43 US_ACTIVE\129309744\V-1of said original transformant that may have been generated by “selfing” the transformant with itself or crossing the transformant or a progeny plant of the transformant that includes the inserted transgenic DNA and event with another plant. For purposes of the present disclosure, the “event” refers to corn event MON87429. As used herein, the term “flanking” in reference to a transgenic event refers to the plant genomic sequence(s) immediately adjacent to the transgenic DNA insertion in the genome of a transformed plant, plant part, plant tissue, or plant cell comprising the transgenic event on the 5´ and / or 3´ side(s) or end(s) of the transgenic event insertion (i.e., the transgenic insertion of corn event MON87429). Likewise, “flanking DNA,” “flanking sequence” or “flanking DNA sequence” each refers to a length of genomic DNA sequence immediately adjacent to the transgenic DNA insertion in the genome of the transformed plant on the 5´ and / or 3´ side(s) or end(s) of the insertion. A “5´ flank” means the corn genomic DNA sequence adjacent to and upstream (or on the 5´ end) of the transgenic DNA insertion. For example, a “5´ flank” can include the corn genomic DNA sequence immediately adjacent to and upstream (on the 5´ end) of the transgenic insertion, or any corn genomic DNA sequence upstream (on the 5´ end) of the transgenic insertion that is not immediately adjacent to the transgenic insertion but is within about 5000 nucleotides, within about 4000 nucleotides, within about 3000 nucleotides, within about 2000 nucleotides, or within about 1000 nucleotides upstream of the transgenic insertion. Likewise, a “3´ flank” means the corn genomic DNA sequence adjacent to and downstream (or on the 3´ end) of the transgenic insert. For example, a “3´ flank” can include the corn genomic DNA sequence immediately adjacent to and downstream (on the 3´ end) of the transgenic insertion, or any corn genomic DNA sequence downstream (on the 3´ end) of the transgenic insertion that is not immediately adjacent to the transgenic insertion but is within about 5000 nucleotides, within about 4000 nucleotides, within about 3000 nucleotides, within about 2000 nucleotides, or within about 1000 nucleotides downstream of the transgenic insertion.

[0134] SEQ ID NOs:14 and 15 are 1,029 and 1,031 nucleotide sequences representing corn (Zea mays) genomic DNA that flanks the transgenic insert at the 5´ and 3´ ends of the insert in corn event MON87429, respectively. SEQ ID NOs:16 and 17 are 5,000 nucleotide sequences representing corn (Zea mays) genomic DNA that flanks the transgenic insert at the 5´ and 3´ ends of the insert, respectively. Nucleotides 4,001-5,000 of SEQ ID NO:16 are identical to nucleotides 1-1,000 of SEQ ID NO: 14. Nucleotides 1-4,000 are based on the genomic sequence of the B73 corn germplasm (Zm-B73-REFERENCE-GRAMENE-4.0, NCBI). Similarly, nucleotides 1-44 US_ACTIVE\129309744\V-11,000 of SEQ ID NO:17 are identical to nucleotides 32-1,031 of SEQ ID NO:15. The remaining nucleotides (1,032-5,000) are based on the genomic sequence of the B73 corn germplasm.

[0135] The present disclosure describes the original transformant plant and progeny of the transformant that include the transgenic DNA and event. Such progeny may be produced by a sexual cross or outcross between plants comprising the same transgenic DNA and event, or between a plant comprising the transgenic DNA and event with another plant, or by any other method known in the art including any cell or tissue culture method, wherein the progeny includes the transgenic DNA and event. Such other plant may be a transgenic plant comprising the same and / or a different transgene or may be a non-transgenic plant, and each parental plant in a cross or outcross may be the same or different germplasm or breeding line. Even after repeated back- crossing to a recurrent parent, the transgenic DNA and event is present in progeny of the cross at the same chromosomal location. Thus, a “transgenic plant” can be the original transformant plant regenerated from the transformed plant cell and comprising the transgenic DNA and event, or a progeny plant of the original transformant plant, which may be separated from the transformant by one or more generations, that retains the transgenic DNA and event at the same specific location and sequence context in the plant’s genome. The transformant or progeny plant may be homozygous or heterozygous for event MON87429. In addition, a “transgenic plant” can include a plant produced from a transformed plant cell or tissue, or from another transgenic plant or plant part, by or using cell or tissue culture methods known in the art. A “transgenic plant” may comprise a plant having a transgene stably inserted into the genome of at least one cell of the plant (i.e., corn event MON87429 in at least one cell of the plant), and the plant may be chimeric or non- chimeric with respect to the transgene and / or event. A transgenic plant is chimeric with respect to a transgene if not all cells of the plant comprise the transgene.

[0136] As used herein, the term “recombinant” refers to a non-natural DNA, protein, or combination that would not normally be found in nature, such as a combination of DNA sequences, proteins that would not naturally occur together, and is the result of human intervention. A “recombinant DNA molecule” is a DNA molecule comprising a combination of DNA sequences that would not naturally occur together and is the result of human intervention. Two or more elements of such combination of DNA sequences may be operably linked to one another. For example, a recombinant DNA molecule may comprise a combination of at least two DNA sequences that are heterologous with respect to each other, such as a DNA molecule that comprises45 US_ACTIVE\129309744\V-1a coding or transcribable DNA sequence operably linked to a heterologous promoter and / or other regulatory expression element(s), and / or a plant genomic DNA sequence comprising all or part of a transgene and a heterologous and flanking genomic sequence(s) adjacent to the transgene, and / or a DNA molecule that is artificially synthesized and comprises a polynucleotide sequence that deviates from any polynucleotide sequence that would normally exist in nature. A recombinant DNA molecule may comprise all or part of a junction sequence of the genome of a plant and all or part of the transgene insertion into the genome of the plant, and / or may comprise a recombinant or heterologous DNA fragment of event MON87429. An example of a recombinant DNA molecule is a DNA molecule comprising at least one of SEQ ID NOs:1-10. As used herein, a recombinant plant, plant part, plant cell or plant tissue is a plant, plant part, plant cell or plant tissue that would not normally exist in nature, is the result of human intervention, and contains a transgene incorporated into the genome of the plant, plant part, plant cell or plant tissue. As a result of such genomic insertion, the recombinant plant is something new and distinctly different from any related wild-type or naturally occurring plant, plant part, plant cell or plant tissue. An example of a recombinant plant, plant part, plant cell or plant tissue is a corn or maize plant, plant part, plant cell or plant tissue containing the event MON87429.

[0137] As used herein, the term “heterologous” in reference to a combination of two or more DNA sequences or elements means that the two or more DNA sequences or elements do not normally exist together as such combination in nature without human intervention. As used herein, the term “heterologous” in reference to a DNA molecule, construct or sequence in relation to a plant, microorganism, plant cell or plant genome means that the DNA molecule, construct or sequence does not exist in nature as part of such plant, microorganism, plant cell or plant genome, and / or does not exist in the same physical or genomic location, context or orientation as part of such plant, microorganism, plant cell or plant genome in nature, without human intervention.

[0138] The present disclosure provides DNA molecules and fragments and their corresponding DNA sequences. The terms “DNA” and “DNA molecule” as used herein refer to a deoxyribonucleic acid (DNA) molecule. A DNA molecule may be of genomic or synthetic origin and / or comprise a recombinant or heterologous DNA molecule or sequence. A DNA molecule may be described in reference to its 5´ (upstream) end and 3´ (downstream) end. As used herein, the term “DNA sequence” refers to the polynucleotide sequence of the DNA molecule – i.e., the sequence of consecutive nucleotides in the DNA molecule. As used herein in reference to46 US_ACTIVE\129309744\V-1nucleotides of a polynucleotide or DNA sequence or molecule, the terms “consecutive” and “contiguous” are interchangeable and synonymous and refer to the 5´ to 3´ order of nucleotides in a polynucleotide or DNA sequence, strand or molecule without any gap or interruption between them. By convention, DNA sequences of the disclosure and fragments thereof are disclosed with reference to the 5´ to 3´ direction of only one strand of the two, anti-parallel and complementary DNA strands of a DNA molecule. By implication and intent, the complementary sequences of the sequences provided here (i.e., the sequences of the complementary, opposing, or antiparallel strand), also referred to in the art as the reverse complementary or reverse complement sequences, are within the scope of the present disclosure and are expressly intended to be within the potential scope of the subject matter as claimed. A DNA molecule, or a fragment derived therefrom, can also be extracted from plant part(s), plant cell(s) and / or tissue(s) or a homogenate, extract or lysate from plant part(s), plant cell(s) and / or tissue(s), or can be produced as an amplicon from extracted, purified or isolated DNA from plant part(s), plant cell(s) and / or tissue(s), or a homogenate, extract or lysate from plant part(s), plant cell(s) and / or tissue(s), which may further comprise event MON87429.

[0139] As used herein, the term “fragment” refers to a smaller piece or sequence of a larger or whole DNA molecule or sequence. For example, a fragment of SEQ ID NO:9 or 10 may include a sequence that is at least about 12 consecutive nucleotides, at least about 13 consecutive nucleotides, at least about 14 consecutive nucleotides, at least about 15 consecutive nucleotides, at least about 16 consecutive nucleotides, at least about 17 consecutive nucleotides, at least about 18 consecutive nucleotides, at least about 19 consecutive nucleotides, at least about 20 consecutive nucleotides, at least about 21 consecutive nucleotides, at least about 22 consecutive nucleotides, at least about 23 consecutive nucleotides, at least about 24 consecutive nucleotides, at least about 25 consecutive nucleotides, at least about 30 consecutive nucleotides, at least about 35 consecutive nucleotides, at least about 40 consecutive nucleotides, at least about 45 consecutive nucleotides, at least about 50 consecutive nucleotides, at least about 60 consecutive nucleotides, at least about 70 consecutive nucleotides, at least about 80 consecutive nucleotides, at least about 90 consecutive nucleotides, at least about 100 consecutive nucleotides, at least about 200 consecutive nucleotides, at least about 300 consecutive nucleotides, at least about 400 consecutive nucleotides, or at least about 500 consecutive nucleotides of the larger, whole or complete DNA molecule or sequence of SEQ ID NO:9 or 10.47 US_ACTIVE\129309744\V-1

[0140] A fragment of the DNA sequence of the 5´ flank (SEQ ID NO:14 or SEQ ID NO:16) or the 3´ flank (SEQ ID NO:15 or SEQ ID NO:17) of corn event MON87429 can comprise at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, or at least 500 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16 or SEQ ID NO:15 or SEQ ID NO:17. It is possible that different maize germplasms may have differences in their genomic sequences, which may include differences in the flanking sequence(s), 5´ flank and / or 3´ flank of corn event MON87429. These differences may result from introgression of the corn event MON87429 into a different germplasm and / or spontaneous, mutagenic or genome editing changes that occur in a given germplasm or line. According to some embodiments, DNA molecules, constructs or polynucleotides are provided comprising a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:14 or 16 or SEQ ID NO:15 or 17, or a fragment of either thereof. According to some embodiments, DNA molecules, constructs or polynucleotides are provided comprising a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, or at least 500 consecutive nucleotides of SEQ ID NO:14 or 16 or SEQ ID NO:15 or 17.

[0141] As used herein, the term “isolated” in reference to a molecule means that the molecule is at least partially separated from other molecules or sequences that are normally associated with the molecule in its native or natural state. In some embodiments, the term “isolated” refers to a DNA molecule that is at least partially separated from the nucleic acids or polynucleotide or DNA sequence(s) that normally flank and are covalently linked to the sequence of the DNA molecule in48 US_ACTIVE\129309744\V-1its native or natural state. An “isolated” DNA molecule may have a DNA sequence corresponding to a portion of the genome of a plant cell without other genomic DNA sequence(s) that normally flank and are covalently linked to the DNA sequence in nature. Such an “isolated” DNA molecule may comprise all or part of a transgene and / or transgenic event, which may comprise all or part of corn event MON87429 or the transgene or expression cassette described herein. Nucleic acid sequences or elements, such as a coding sequence, intron sequence, untranslated leader sequence, promoter sequence, transcriptional termination sequence, and the like, that are naturally found within the DNA of the genome of an organism are not considered to be “isolated” so long as the element is within the genome of the organism and at the location within the genome in which it is naturally found. However, each of these elements, and subparts of these elements, would be “isolated” within the scope of this disclosure so long as the element or subpart is not within the genome of the organism, and at the location within the genome of the organism, in which it is naturally found. An “isolated” DNA molecule may be any recombinant DNA molecule or amplification product or amplicon, and / or may comprise any DNA sequence removed from its natural or biological state and covalently fused to another DNA molecule or sequence with which it is not associated in nature. Such an isolated DNA molecule could be created by the use of biotechnology techniques, such as by making a recombinant DNA or integrating a foreign or heterologous DNA molecule into the chromosome of a cell, plant, or seed. Thus, any DNA molecule comprising a transgenic, recombinant, chimeric or artificial nucleotide sequence, transgene or expression cassette would be considered to be an “isolated” DNA molecule since these sequences are not naturally occurring, regardless of whether the sequence, transgene or expression cassette is present within a plasmid, vector or construct used to transform plant cells, within the genome of a plant, plant part, plant tissue, or plant cell, or is present in detectable amounts in tissues, progeny, biological samples or commodity products derived from a plant, plant part, plant tissue, or plant cell. A recombinant DNA molecule or sequence, or any fragment derived therefrom, comprising all or part of a transgene or junction sequence of corn event would therefore also be considered to be “isolated.” An “isolated” DNA molecule may be extracted or purified from a transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be present in a homogenate, extract or lysate from any such transgenic plant(s), plant part(s), plant cell(s) and / or tissue(s), or may be produced as an amplicon or amplification product from plant genomic DNA and / or extracted or purified DNA from transgenic plant(s), plant part(s), plant cell(s) and / or49 US_ACTIVE\129309744\V-1tissue(s), or a homogenate, extract or lysate from plant(s), plant part(s), plant cell(s) and / or tissue(s). For the purposes of this disclosure, any transgenic polynucleotide or DNA sequence, i.e., the nucleotide sequence of the DNA inserted into the genome of a plant or bacterium, or present in an extrachromosomal vector, would be considered to be an “isolated” nucleotide or DNA sequence whether it is present within the plasmid or similar structure used to transform the cells, within the genome of the plant or bacterium, or present in detectable amounts in tissues, progeny, biological samples or commodity products derived from the plant or bacterium. An “isolated” DNA molecule is a chemical or biochemical molecule, regardless of whether the molecule is referred to as a nucleic acid, a nucleic acid sequence, a polynucleotide sequence, a DNA sequence, a nucleic acid molecule, a polynucleotide molecule, a DNA molecule, or the like. An “isolated” molecule can provide industrial applicability when present in a plant cell or in a plant genome or when present outside of a plant cell, and therefore, provides and exhibits (and is intended to provide and exhibit) utility regardless of where the molecule is located.

[0142] The phosphodiester bond linkage between one end of a transgenic insert (or insertion) into the genome of a plant and the flanking corn genomic DNA is referred to as a “junction.” In other words, a “junction’ is the connection point or covalent linkage of one end of a transgenic insert and the flanking genomic DNA. One junction is found at the 5´ end of the transgenic insertion and the other is found at the 3´ end of the transgenic insert, referred to herein as the 5´ and 3´ junction, respectively. A “junction sequence” refers to a DNA sequence of any length of consecutive nucleotides that spans the 5´ or 3´ junction of a transgenic event in the plant genome. For a “junction sequence” to be specific to a junction between a transgenic event and a flanking genomic sequence, the junction sequence will generally comprise a sufficient number of consecutive nucleotides at one end of the insertion and a sufficient number of consecutive nucleotides of the flanking genomic sequence. According to some embodiments, a “junction sequence” may comprise (i) at least five (5) consecutive nucleotides, at least ten (10) consecutive nucleotides, at least fifteen (15) consecutive nucleotides, at least twenty (20) consecutive nucleotides, or at least thirty (30) consecutive nucleotides at one end of the insertion and (ii) at least five (5) consecutive nucleotides, at least ten (10) consecutive nucleotides, at least fifteen (15) consecutive nucleotides, at least twenty (20) consecutive nucleotides, or at least thirty (30) consecutive nucleotides of flanking genomic DNA sequence, although it is understood that any length of consecutive nucleotides spanning a junction of a transgenic event in a plant genome may50 US_ACTIVE\129309744\V-1be a junction sequence. A variety of junction sequences of corn event MON87429 can be determined by one of skill in the art using SEQ ID NO:10. Examples of junction sequences of event MON87429 are provided as SEQ ID NOs:1-8. FIG. 1 illustrates the physical arrangement and locations of the junction sequences, arranged from 5´ to 3´ (left to right), relative to SEQ ID NO:10. The junction sequences of event MON87429 may be present as part of the genome of a corn plant, plant part, plant seed, or plant tissue or cell containing event MON87429, a DNA molecule containing all or part of event MON87429, or a microorganism containing event MON87429. The identification of any one or more of the junction sequences in a DNA molecule or sample from a plant, plant part, plant seed, or plant tissue or cell indicates that the plant, plant part, plant seed, or plant tissue or cell contains or comprises event MON87429, or the DNA molecule contains or comprises event MON87429 or was obtained from a corn plant, plant part, plant seed, or plant tissue or cell containing or comprising event MON87429, and is diagnostic in each case for the presence of event MON87429.

[0143] The junction sequences described herein are diagnostic for the presence of all or part of event MON87429 and / or a DNA molecule comprising all or part of the construct or expression cassettes described herein. Thus, the identification or detection, directly or indirectly, of one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10 in a sample or DNA molecule derived from a corn plant, corn plant part, corn seed, or corn tissue or cell, or a commodity product from a corn plant, corn plant part, corn seed, or corn tissue or cell, is diagnostic that the corn plant, corn plant part, corn seed, or corn tissue or cell, or a commodity product from a corn plant, corn plant part, corn seed, or corn tissue or cell has or comprises all or part of corn event MON87429. The identification or detection, directly or indirectly, of a 5´ junction sequence and a 3´ junction sequence (each as provided or described herein) in a sample or DNA molecule derived from a corn plant, corn plant part, corn seed, or corn tissue or cell, or a commodity product from a corn plant, corn plant part, corn seed, or corn tissue or cell, is diagnostic that the corn plant, corn plant part, corn seed, or corn tissue or cell, or a commodity product from a corn plant, corn plant part, corn seed, or corn tissue or cell has or comprises corn event MON87429 The present disclosure thus provides a DNA molecule that contains at least one of the nucleotide sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. Any segment of DNA derived from51 US_ACTIVE\129309744\V-1transgenic corn event MON87429 that is sufficient to include at least one of the sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10 is within the scope of the present disclosure. In addition, any DNA or polynucleotide molecule or sequence comprising a sequence complementary to any of the sequences described herein is also within the scope of the present disclosure.

[0144] The disclosure provides DNA, polynucleotide or nucleic acid molecules, which may be single or double stranded, that can be used either as primers or probes for detecting the presence of DNA comprising all or part of event MON87429 or a modified event MON87429 in a sample derived from a corn plant, corn plant part, corn seed, or corn tissue or cell, or a commodity product from a corn plant, corn plant part, corn seed, or corn tissue or cell. Such primers or probes are specific for a target nucleic acid, polynucleotide or DNA sequence and, as such, are useful for the identification of corn event MON87429 or a modified event MON87429 nucleic acid, polynucleotide or DNA sequence by the methods described herein. A primer or probe can hybridize to a target nucleic acid, polynucleotide or DNA sequence to allow for specific detection or amplification of a nucleic acid, polynucleotide or DNA molecule or sequence that comprises, or is covalently linked and associated with, the target nucleic acid, polynucleotide or DNA sequence. According to present embodiments, the primers and / or probe may be chosen to identify and distinguish detection of a particular transgenic event and not only the presence of a transgene in a plant genome. The target nucleic acid, polynucleotide or DNA molecule or sequence may comprise all or part of corn event MON87429 or a modified event MON87429, a junction sequence and / or flanking genomic DNA. Probes and primers according to the present disclosure may have (i) complete or 100% sequence complementarity (i.e., 100% complementary) to a target DNA sequence or (ii) incomplete sequence complementarity to a target DNA sequence, such as at least 60% complementary, at least 65% complementary, at least 70% complementary, at least 75% complementary, at least 80% complementary, at least 85% complementary, at least 90% complementary, at least 95% complementary, or at least 99% complementary to the target DNA sequence as long as the probe or primer has sufficient complementarity to the target DNA sequence to hybridize to the target DNA sequence under stringent hybridization conditions that are suitable and necessary for use of the probe or primer in the relevant amplification or detection assay,52 US_ACTIVE\129309744\V-1reaction or method. As understood in the art, the percentage complementarity of a primer or probe may be lower if the length of the primer or probe is longer and depends on the stringency and use.

[0145] A “probe” is a nucleic acid molecule that is complementary to a strand of target nucleic acid and is useful in hybridization methods. A probe may be attached to a conventional detectable label or reporter molecule, e.g., a radioactive isotope, ligand, chemiluminescent agent, or enzyme. Such a probe is complementary to a strand of a target nucleic acid and, in the case of the present invention, to a strand of DNA from event MON87429 or a modified event MON87429 whether from an event MON87429 or a modified event MON87429 containing plant or from a sample that includes event MON87429 DNA or a modified event MON87429 DNA. Probes according to the present invention include not only deoxyribonucleic or ribonucleic acids, but also polyamides and other probe materials that bind specifically to a target DNA sequence and can be used to detect the presence of that target DNA sequence. An illustrative DNA sequence useful as a probe for detecting corn event MON87429 is provided as SEQ ID NO:13 (PB50370). A “probe” may also be used to bind a template DNA in a sample comprising all or part of a DNA or nucleotide sequence of corn event MON87429 or a modified event MON87429 to purify the template DNA from the remainder of the sample using purification methods or techniques known in the art, for example, if the probe is bound or can be bound to a substrate or a particle or bead that can be purified or separated. Such a template DNA may comprise all or part of a DNA or nucleotide sequence of corn event MON87429 or a modified event MON87429, or a portion or fragment thereof, such as a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a complement thereof.

[0146] A “primer” is typically a DNA molecule that is designed for use in specific annealing or hybridization methods that involve thermal amplification. A pair of primers may be used with template DNA (such as a sample of corn genomic DNA) in a thermal amplification (such as polymerase chain reaction (PCR)) to produce an amplicon, where the amplicon produced from such reaction would have a DNA sequence corresponding to sequence of the template DNA located between the two sites where the primers hybridized to the template. As understood in the art, an “amplification product” or “amplicon” is a DNA molecule or segment produced by an amplification reaction. Amplification or amplifying refers to making multiple copies of a target DNA molecule or segment from a template DNA. A single “primer” may also be used to initiate53 US_ACTIVE\129309744\V-1a sequencing reaction to determine a DNA sequence of a template DNA according to sequencing methods known in the art. Such a sequencing reaction may be used to determine the presence or absence of a DNA molecule or nucleotide sequence, or a portion or fragment thereof, from corn event MON87429 or a modified event MON87429. Such a template DNA may comprise all or part of a DNA or nucleotide sequence of corn event MON87429 or a modified event MON87429, or a portion or fragment thereof, such as a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a complement thereof.

[0147] DNA amplification reactions, methods and techniques are known to those skilled in art. DNA amplification can be accomplished by any of the various nucleic acid amplification methods known in the art, including thermal and isothermal amplification methods including the polymerase chain reaction or PCR. Amplification methods are known in the art and are described, inter alia, in U.S. Patent Nos.4,683,195 and 4,683,202 and in PCR Protocols: A Guide to Methods and Applications, ed. Innis et al., Academic Press, San Diego, 1990. PCR amplification methods have been developed to amplify up to 22 kb (kilobase) of genomic DNA and up to 42 kb of bacteriophage DNA (Cheng et al., Proc. Natl. Acad. Sci. USA 91:5695-5699, 1994). These methods as well as other methods known in the art of DNA amplification may be used in the practice of the present invention. Examples of DNA amplification methods include PCR, Recombinase Polymerase Amplification (RPA) (see for example U.S. Pat No. 7,485,428), Strand Displacement Amplification (SDA) (see for example, U.S. Pat. Nos. 5,455,166 and 5,470,723), Transcription-Mediated Amplification (TMA) (see for example, Guatelli et al., Proc. Natl. Acad. Sci. USA 87:1874-1878, 1990), Rolling Circle Amplification (RCA) (see for example, Fire and Xu, Proc. Natl. Acad Sci. USA 92:4641-4645, 1995; Lui, et al., J. Am. Chem. Soc.118:1587-1594, 1996; Lizardi, et al., Nature Genetics 19:225-232, 1998; U.S. Pat. Nos.5,714,320 and 6,235,502), Helicase Dependent Amplification (HDA) (see for example Vincent et al., EMBO Reports 5(8): 795-800, 2004; U.S. Pat. No. 7,282,328), and Multiple Displacement Amplification (MDA) (see for example Dean et al., Proc. Natl. Acad Sci. USA 99:5261-5266, 2002). A sequence of the heterologous DNA insert and / or flanking genomic DNA sequence from corn event MON87429 or a modified event MON87429 can be verified or tested by amplifying such DNA molecules from corn seed containing event MON87429 DNA or a modified event MON87429 or corn plants grown from the corn seed containing event MON87429 DNA or a modified event MON87429,54 US_ACTIVE\129309744\V-1using primers derived from the sequences provided herein, followed by standard DNA sequencing of the PCR amplicon or a cloned DNA fragment thereof.

[0148] According to present embodiments, the sequence of an amplicon of an amplification reaction may comprise one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a fragment thereof. According to present embodiments, the sequence of an amplicon comprises at least one junction sequence or two junction sequences, such as a 5´ junction sequence and / or a 3´ junction sequence for corn event MON87429 or a modified event MON87429.

[0149] A primer is typically designed to hybridize in a sequence-specific manner to a complementary target DNA strand to form a hybrid between the primer and target DNA strand, and the primer hybridized or bound to the complementary target DNA strand is a point of recognition for a polymerase to begin extension of the primer (i.e., polymerization of additional nucleotides into a lengthening nucleotide molecule) using as a template the target DNA strand. Primer pairs refer to use of two primers binding opposite strands of a double stranded DNA or polynucleotide segment for the purpose of amplifying the polynucleotide or DNA segment between the positions targeted for binding by the individual primers of the primer pair to the original template DNA or an amplicon of the amplification reaction, typically in a thermal cycling amplification reaction or other conventional DNA amplification method. Primer pairs are typically designed to hybridize to different nearby target positions of a template DNA molecule on opposing strands of the template DNA molecule such that the intervening region or sequence between the two primers can be specifically amplified for use or detection through multiple rounds of amplification. Exemplary DNA molecules useful as primers are provided as SEQ ID NO:11 and SEQ ID NO:12.

[0150] The primer pair SEQ ID NO:11 and SEQ ID NO:12 are useful as a first DNA molecule and a second DNA molecule that is different from the first DNA molecule, and both are each of sufficient length of contiguous nucleotides of SEQ ID NO:10 to function as DNA primers that, when used together in a thermal amplification reaction with template DNA derived from corn event MON87429, to produce an amplicon diagnostic for corn event MON87429 DNA in a sample. The primer pair SEQ ID NO:11 and SEQ ID NO:12 are useful as a first DNA molecule and a second DNA molecule that is different from the first DNA molecule, and both are each of sufficient length of contiguous nucleotides of SEQ ID NO:10 to function as DNA primers that,55 US_ACTIVE\129309744\V-1when used together in a thermal amplification reaction with template DNA derived from corn event MON87429, produce an amplicon diagnostic for the zygosity of corn event MON87429 DNA in a sample.

[0151] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises at least one of the nucleotide sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, or at least 90 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, or SEQ ID NO:6. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that (i) contains or comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of any one of SEQ ID NOs:1-10, (ii) is at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 nucleotides in length, and (iii) comprises nucleotides 1,029-1,030 and / or 15,037–15,038 of SEQ ID NO: 10.

[0152] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) four expression cassettes that encode PAT, DMO, FT_T and EPSPS, and (ii) a junction sequence. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a 5´ flank and / or a 3´ flank sequence. The expression cassette may comprise in operable linkage: (a) a promoter sequence (b)56 US_ACTIVE\129309744\V-1a transcribable DNA sequence encoding a PAT, or DMO, or FT or EPSPS that provide tolerance to herbicides, and (c) a transcription termination or 3´ UTR sequence. The expression cassette may further comprise any of the elements described in Table 1, which may be operably linked.

[0153] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a polynucleotide sequence comprising one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of one or more of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10.

[0154] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16 or SEQ ID NO:15 or SEQ ID NO:17. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:14 or 16 or57 US_ACTIVE\129309744\V-1SEQ ID NO:15 or 17, or a fragment of either thereof. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16 or SEQ ID NO:15 or 17. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS and (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:18-117. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0155] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, (ii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16, and (iii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at58 US_ACTIVE\129309744\V-1least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or SEQ ID NO:17. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, (ii) a sequence or flanking sequence, or a 5´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:14 or 16, or a fragment of either thereof, and (iii) a sequence or flanking sequence, or a 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:15 or 17, or a fragment of either thereof. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, and (ii) a sequence or flanking sequence, or a 5´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16, and (iii) a sequence or flanking sequence, or a 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at59 US_ACTIVE\129309744\V-1least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) expression cassettes that encode PAT, DMO, FT and EPSPS, (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:18-117, and (iii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0156] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end at least 50 consecutive nucleotides of SEQ ID NO:14 or 16. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end at least 50 consecutive nucleotides of SEQ ID NO:15 or 17. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end a polynucleotide sequence selected from SEQ ID NOs:18-117. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end a polynucleotide sequence selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0157] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, wherein the DNA molecule,60 US_ACTIVE\129309744\V-1construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end a polynucleotide sequence selected from SEQ ID NOs:18-117, and / or wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end a polynucleotide sequence selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0158] According to present embodiments, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16 at the 5´ end of a DNA molecule, construct, segment, amplicon, fragment or polynucleotide may be immediately adjacent to and upstream (on the 5´ end) of the transgenic insertion, or may not be immediately adjacent to, but further upstream (on the 5´ end) and within about 5000 nucleotides, within about 4000 nucleotides, within about 3000 nucleotides, within about 2000 nucleotides, or within about 1000 nucleotides of the transgenic insertion. According to present embodiments, at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or SEQ ID NO:17 at the 3´ end of a DNA molecule, construct, segment, amplicon, fragment or polynucleotide may be immediately adjacent to and downstream (on the 3´ end) of the transgenic insertion, or may not be immediately adjacent to but further downstream (on the 3´ end) and within about 5000 nucleotides, within about 4000 nucleotides, within about 3000 nucleotides, within about 2000 nucleotides, or within about 1000 nucleotides of the transgenic insertion. Any sequence comprising at least 50 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16, or at least 50 consecutive nucleotides of SEQ ID NO:15 or SEQ ID NO:17 is within the scope of the present disclosure. A DNA molecule, construct, segment,61 US_ACTIVE\129309744\V-1amplicon, fragment or polynucleotide can comprise at the 5´ and / or 3´ end of the construct (i) at least 50 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16; and / or (ii) at least 50 consecutive nucleotides of SEQ ID NO:15 or SEQ ID NO:17, respectively. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0159] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a junction sequence. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a 5´ flank and / or a 3´ flank sequence. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0160] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at62 US_ACTIVE\129309744\V-1least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16 or SEQ ID NO:15 or SEQ ID NO:17. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO: 9, and (ii) a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:14 or 16 or SEQ ID NO:15 or 17, or a fragment of either thereof. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0161] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a sequence or flanking sequence, or a 5´ flank or 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at63 US_ACTIVE\129309744\V-1least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16 or SEQ ID NO:15 or 17. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:18-117. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO: 10.

[0162] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:10.

[0163] A DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, (ii) a polynucleotide sequence64 US_ACTIVE\129309744\V-1comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or SEQ ID NO:16, and (iii) a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or SEQ ID NO:17. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, (ii) a sequence or flanking sequence, or a 5´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:14 or 16, or a fragment of either thereof, and (iii) a sequence or flanking sequence, or a 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to SEQ ID NO:15 or 17, or a fragment of either thereof. According to some embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least65 US_ACTIVE\129309744\V-1150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, and (ii) a sequence or flanking sequence, or a 5´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16, and (iii) a sequence or flanking sequence, or a 3´ flank, that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:10.

[0164] Also provided is a DNA molecule, construct, segment, amplicon, fragment or polynucleotide that contains or comprises (i) SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, (ii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:18-117, and (iii) a polynucleotide sequence comprising one or more polynucleotide sequences selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment,66 US_ACTIVE\129309744\V-1amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:10.

[0165] Also provided is a DNA molecule, construct, segment, amplicon, fragment or polynucleotide that contains or comprises SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end at least 50 consecutive nucleotides of SEQ ID NO:18-117. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end at least 50 consecutive nucleotides of SEQ ID NO:15 or 17. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises expression cassettes that encode PAT, DMO, FT and EPSPS, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end a polynucleotide sequence selected from SEQ ID NOs:18-117. According to present embodiments, a DNA molecule, construct, segment, amplicon, fragment or polynucleotide is provided that contains or comprises SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, wherein the DNA molecule, construct, segment, amplicon, fragment67 US_ACTIVE\129309744\V-1or polynucleotide comprises at its 3´ end a polynucleotide sequence selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:10.

[0166] Also provided is a DNA molecule, construct, segment, amplicon, fragment or polynucleotide that contains or comprises SEQ ID NO:9 or a polynucleotide sequence comprising at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:9, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 5´ end a polynucleotide sequence selected from SEQ ID NOs:18- 117, and / or wherein the DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at its 3´ end a polynucleotide sequence selected from SEQ ID NOs:118-217. The DNA molecule, construct, segment, amplicon, fragment or polynucleotide comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:10.

[0167] A corn plant, plant part, plant seed, plant tissue, plant cell or commodity product is provided comprising any DNA molecule, construct, segment, fragment or polynucleotide described herein. A corn plant comprising a construct as described herein may be further characterized as having tolerance to at least one herbicide selected from the group consisting of glufosinate, dicamba, 2,4-D, and glyphosate, relative to a non-transgenic control plant.

[0168] To detect the presence or absence of corn event MON87429 or a modified corn event MON87429, the target positions and / or the intervening region or sequence of a template DNA molecule may comprise at least one junction sequence and / or at least a portion of the insert of corn event MON87429 or a modified corn event MON87429. To detect the absence of corn event68 US_ACTIVE\129309744\V-1MON87429 or a modified corn event MON87429, the target positions and / or the intervening region or sequence of a template DNA molecule may comprise corn genomic DNA that does not include a junction sequence or any portion of the insert of corn event MON87429 or a modified corn event MON87429. Thus, the presence or absence of an amplicon with a primer pair may be diagnostic of the presence or absence, respectively, of corn event MON87429 or a modified corn event MON87429 in a DNA molecule or sample, or vice versa. This may also be possible with more than one primer pair. For example, a first primer pair may produce a first amplicon if corn event MON87429 or a modified corn event MON87429 is present, and a second primer pair may produce a second amplicon if corn event MON87429 or the modified corn event MON87429 is absent or not present. Alternatively, the size of an amplicon produced in an amplification reaction may also be diagnostic of the presence or absence of corn event MON87429 or a modified corn event MON87429 in a DNA molecule or sample – e.g., a primer pair may produce a first amplicon of a first size if corn event MON87429 or a modified corn event MON87429 is present or a second amplicon of a second size if corn event MON87429 or the modified corn event MON87429 is absent and not present; or a first primer pair may produce a first amplicon of a first size if corn event MON87429 or a modified corn event MON87429 is present, and a second primer pair may produce a second amplicon of a second size if corn event MON87429 or the modified corn event MON87429 is absent or not present. According to some of these embodiments, at least two primer pairs may be used wherein at least one of the primer pairs is used as an internal control and is not associated with corn event MON87429 or a modified corn event MON87429.

[0169] A primer pair to detect the presence of all or part of corn event MON87429 or a modified corn event MON87429 in a DNA molecule or sample comprises a first primer and a second primer, wherein the first primer is identical (or complementary) to a 5´ flanking genomic DNA sequence and the second primer is complementary (or identical) to a sequence within the transgenic insert; or wherein the first primer is identical (or complementary) to a 5´ flanking genomic DNA sequence and the second primer is complementary (or identical) to a 3´ flanking genomic DNA sequence; or wherein the first primer is identical (or complementary) to a sequence within the transgenic insert and the second primer is complementary (or identical) to a 3´ flanking genomic DNA sequence.

[0170] Exemplary DNA molecules useful as primers are provided as SEQ ID NO:11 and SEQ ID NO:12. The primer pair SEQ ID NO:11 and SEQ ID NO:12 can be useful as a first DNA molecule69 US_ACTIVE\129309744\V-1or primer and a second DNA molecule or primer, wherein each primer has sufficient length of consecutive nucleotides of SEQ ID NO:10 or a sequence complementary to SEQ ID NO:10 to function as DNA primers that, when used together in an amplification reaction with template DNA derived from corn event MON87429, hybridize to opposite strands of the template DNA and produce an amplicon diagnostic for corn event MON87429 DNA in a sample.

[0171] DNA probes and DNA primers are generally eleven (11) polynucleotides or more in length, often eighteen (18) polynucleotides or more, twenty-four (24) polynucleotides or more, or thirty (30) polynucleotides or more. Such probes and primers are selected to be of sufficient length to hybridize specifically to a target sequence under high stringency hybridization conditions. Preferably, probes and primers according to the present invention have complete sequence similarity with the target sequence, although probes differing from the target sequence that retain the ability to hybridize to target sequences may be designed by conventional methods.

[0172] The nucleic acid probes and primers of the present invention hybridize under stringent conditions to a target DNA molecule. Any conventional nucleic acid hybridization or amplification method can be used to identify the presence of DNA from a transgenic plant in a sample. Polynucleic acid molecules also referred to as nucleic acid segments or fragments thereof are capable of specifically hybridizing to other nucleic acid molecules under certain circumstances.

[0173] As used herein, two polynucleic acid molecules are said to be capable of specifically hybridizing to one another if the two molecules are capable of forming an anti-parallel, double- stranded nucleic acid structure. A nucleic acid molecule is said to be the “complement” of another nucleic acid molecule if they exhibit complete complementarity. As used herein, molecules are said to exhibit “complete complementarity” when every nucleotide of one of the molecules is complementary to a nucleotide of the other. Two molecules are said to be “minimally complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under at least conventional "low-stringency" conditions. Similarly, the molecules are said to be “complementary” if they can hybridize to one another with sufficient stability to permit them to remain annealed to one another under conventional "high-stringency" conditions. Conventional stringency conditions are described by Sambrook et al., 1989, and by Haymes et al., In: Nucleic Acid Hybridization, A Practical Approach, IRL Press, Washington, DC (1985). Departures from complete complementarity are therefore permissible, as long as such departures do not completely preclude the capacity of the molecules to form a double-stranded70 US_ACTIVE\129309744\V-1structure. In order for a nucleic acid molecule to serve as a primer or probe it needs only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed.

[0174] As used herein, a substantially homologous sequence is a nucleic acid sequence that will specifically hybridize to the complement of the nucleic acid sequence to which it is being compared under high stringency conditions. Appropriate stringency conditions that promote DNA hybridization, for example, 6.0 x sodium chloride / sodium citrate (SSC) at about 45°C, followed by a wash of 2.0 x SSC at 50°C, are known to those skilled in the art or can be found in Current Protocols in Molecular Biology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6. For example, the salt concentration in the wash step can be selected from a low stringency of about 2.0 x SSC at 50°C to a high stringency of about 0.2 x SSC at 50°C. In addition, the temperature in the wash step can be increased from low stringency conditions at room temperature, about 22°C, to high stringency conditions at about 65°C. Both temperature and salt may be varied, or either the temperature or the salt concentration may be held constant while the other variable is changed. In a preferred embodiment, a polynucleic acid of the present invention will specifically hybridize to one or more of the nucleic acid molecules set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10, or complements thereof or fragments thereof under moderately stringent conditions, for example at about 2.0 x SSC and about 65°C. A nucleic acid of the present disclosure will specifically hybridize to one or more of the nucleic acid molecules set forth in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, or SEQ ID NO:10, or complements or fragments thereof under high stringency conditions. In one aspect of the present disclosure, a preferred marker nucleic acid molecule of the present disclosure has the nucleic acid sequence set forth in SEQ ID NO:1, or SEQ ID NO:2, or SEQ ID NO:3, or SEQ ID NO:4, or SEQ ID NO:5, or SEQ ID NO:6, or SEQ ID NO:7, or SEQ ID NO:8, or SEQ ID NO:9, or SEQ ID NO:10, or complements thereof, or fragments thereof. The hybridization of the probe to the target DNA molecule can be detected by any number of methods known to those skilled in the art, these can include, but are not limited to, fluorescent tags, radioactive tags, antibody-based tags, and chemiluminescent tags.

[0175] Regarding the amplification of a target nucleic acid sequence (e.g., by PCR) using a particular amplification primer pair, "stringent conditions" are conditions that permit the primer71 US_ACTIVE\129309744\V-1pair to hybridize only to the target nucleic acid sequence to which a primer having the corresponding wild-type sequence (or its complement) would bind and preferably to produce a unique amplification product, the amplicon, in a DNA thermal amplification reaction.

[0176] The term "specific for (a target sequence)" indicates that a probe or primer hybridizes under stringent hybridization conditions only to the target sequence in a sample comprising the target sequence.

[0177] As used herein, “amplified DNA” or “amplicon” refers to the product of polynucleic acid amplification method directed to a target polynucleic acid molecule that is part of a polynucleic acid template. For example, to determine whether a corn plant resulting from a sexual cross contains transgenic plant genomic DNA from a corn plant comprising event MON87429 or a modified corn event MON87429 of the present invention, DNA that is extracted from a corn plant tissue sample may be subjected to a polynucleic acid amplification method using a primer pair that includes a first primer derived from a genomic DNA sequence in the region flanking the heterologous inserted DNA of event MON87429 or a modified corn event MON87429 and is elongated by polymerase 5´ to 3´ in the direction of the inserted DNA. The second primer is derived from the heterologous inserted DNA molecule is elongated by the polymerase 5´ to 3´ in the direction of the flanking genomic DNA from which the first primer is derived. The amplicon may range in length from the combined length of the primer pair plus one nucleotide base pair, or plus about fifty nucleotide base pairs, or plus about two hundred-fifty nucleotide base pairs, or plus about four hundred-fifty nucleotide base pairs or more. Alternatively, a primer pair can be derived from genomic sequence on both sides of the inserted heterologous DNA so as to produce an amplicon that includes the entire insert polynucleotide sequence (e.g., a forward primer isolated from the genomic portion on the 5´ end of SEQ ID NO:10 and a reverse primer isolated from the genomic portion on the 3´ end of SEQ ID NO:10 that amplifies a DNA molecule comprising the inserted DNA sequence (SEQ ID NO:9)). A member of a primer pair derived from the plant genomic sequence adjacent to the inserted transgenic DNA is located a distance from the inserted DNA sequence, this distance can range from one nucleotide base pair up to about twenty thousand nucleotide base pairs. The use of the term “amplicon” specifically excludes primer dimers that may be formed in the DNA thermal amplification reaction.

[0178] For practical purposes, one should design primers which produce amplicons of a limited size range, for example, between 100 to 1000 bases. Smaller (shorter polynucleotide length) sized72 US_ACTIVE\129309744\V-1amplicons in general are more reliably produced in thermal amplification reactions, allow for shorter cycle times, and can be easily separated and visualized on agarose gels or adapted for use in endpoint TaqMan®-like assays. Smaller amplicons can be produced and detected by methods known in the art of DNA amplicon detection. In addition, amplicons produced using the primer pairs can be cloned into vectors, propagated, isolated, and sequenced or can be sequenced directly with methods well established in the art. Any primer pair derived from the combination of SEQ ID NO:14 and SEQ ID NO:9 or the combination of SEQ ID NO:15 and SEQ ID NO:9 that are useful in a DNA amplification method to produce an amplicon diagnostic for event MON87429 or a modified corn event MON87429, or progeny thereof is an aspect of the invention. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:14, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for event MON87429 or a modified corn event MON87429 or progeny thereof is an aspect of the disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:15, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for plants comprising event MON87429 or a modified corn event MON87429 or progeny thereof is an aspect of the disclosure. Any single isolated DNA polynucleotide primer molecule comprising at least 15 contiguous nucleotides of SEQ ID NO:9, or its complement that is useful in a DNA amplification method to produce an amplicon diagnostic for event MON87429 or a modified corn event MON87429 or progeny thereof is an aspect of the disclosure.

[0179] Polynucleic acid amplification can be accomplished by any of the various polynucleic acid amplification methods known in the art, including the polymerase chain reaction (PCR). Amplification methods are known in the art and are described, inter alia, in U.S. Patent Nos. 4,683,195 and 4,683,202 and in PCR Protocols: A Guide to Methods and Applications, ed. Innis et al., Academic Press, San Diego, 1990. PCR amplification methods have been developed to amplify up to 22 kb (kilobase) of genomic DNA and up to 42 kb of bacteriophage DNA (Cheng et al., Proc. Natl. Acad. Sci. USA 91:5695-5699, 1994). These methods as well as other methods known in the art of DNA amplification may be used in the practice of the present invention. The sequence of the heterologous DNA insert or flanking genomic DNA sequence from corn event MON87429 can be verified (and corrected if necessary) by amplifying such DNA molecules from corn seed containing event MON87429 DNA or corn plants grown from the corn seed containing73 US_ACTIVE\129309744\V-1event MON87429 DNA deposited with the ATCC having accession No. PTA-124635, using primers derived from the sequences provided herein, followed by standard DNA sequencing of the PCR amplicon or cloned DNA fragments thereof.

[0180] A diagnostic amplicon produced by the methods described herein may be detected by a plurality of techniques known in the art, such as sequencing, restriction mapping, Northern analysis, Southern analysis, or any other suitable polynucleotide or DNA hybridization, blotting, polymerization and / or amplification-based approach or technique. One method is Genetic Bit Analysis (Nikiforov et al., Nucleic Acid Res. 22:4167-4175, 1994) where a DNA oligonucleotide is designed that overlaps both the adjacent flanking genomic DNA sequence and the inserted DNA sequence – i.e., a junction sequence. The oligonucleotide is immobilized in wells of a microtiter plate. Following PCR of the region of interest (using, for example, one primer in the inserted sequence and one in the adjacent flanking genomic sequence), a single-stranded PCR product can be hybridized to the immobilized oligonucleotide and serve as a template for a single base extension reaction using a DNA polymerase and labeled dideoxynucleotide triphosphates (ddNTPs) specific for the expected next base. Readout may be fluorescent or ELISA-based. A signal indicates presence of the transgene / genomic junction sequence due to successful amplification, hybridization, and single base extension.

[0181] Another method is the pyrosequencing technique as described by Winge (Innov. Pharma. Tech. 00:18-24, 2000). In this method, an oligonucleotide is designed that overlaps the adjacent genomic DNA and insert DNA junction. The oligonucleotide is hybridized to single-stranded PCR product from the region of interest (one primer in the inserted sequence and one in the flanking genomic sequence) and incubated in the presence of a DNA polymerase, ATP, sulfurylase, luciferase, apyrase, adenosine 5´ phosphosulfate and luciferin. dNTPs are added individually and the incorporation results in a light signal that is measured. A light signal indicates the presence of the transgene / genomic sequence due to successful amplification, hybridization, and single or multi-base extension.

[0182] Fluorescence polarization as described by Chen, et al., (Genome Res. 9:492-498, 1999) is a method that can be used to detect the amplicon of the present invention. Using this method an oligonucleotide is designed that overlaps the genomic flanking and inserted DNA junction. The oligonucleotide is hybridized to single-stranded PCR product from the region of interest (one primer in the inserted DNA and one in the flanking genomic DNA sequence) and incubated in the74 US_ACTIVE\129309744\V-1presence of a DNA polymerase and a fluorescent-labeled ddNTP. Single base extension results in incorporation of the ddNTP. Incorporation can be measured as a change in polarization using a fluorometer. A change in polarization indicates the presence of the transgene / genomic sequence due to successful amplification, hybridization, and single base extension.

[0183] Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs (i.e., in real time). Data is collected throughout the PCR process, rather than at the end of the PCR. In real-time PCR, reactions are characterized by the point in time during cycling when amplification of a target is first detected rather than the amount of target accumulated after a fixed number of cycles. In a real-time PCR assay, a positive reaction is detected by accumulation of a fluorescent signal. The higher the starting copy number of the nucleic acid target, the sooner a significant increase in fluorescence is observed. The cycle threshold (Ct value) is defined as the number of cycles required for the fluorescent signal to cross the threshold (i.e., exceeds background level). Ct levels are inversely proportional to the amount of target nucleic acid in the sample (i.e., the lower the Ct value, the greater the amount of target nucleic acid in the sample).

[0184] TaqMan® (PE Applied Biosystems, Foster City, CA) is described as a method of detecting and quantifying the presence of a DNA sequence using real-time PCR and is fully understood in the instructions provided by the manufacturer. Briefly, a FRET oligonucleotide probe is designed that overlaps the genomic flanking and insert DNA junction. The FRET probe and PCR primers (one primer in the insert DNA sequence and one in the flanking genomic sequence) are cycled in the presence of a thermal stable polymerase and dNTPs. Hybridization of the FRET probe results in cleavage and release of the fluorescent moiety away from the quenching moiety on the FRET probe. A fluorescent signal indicates the presence of the transgene / genomic sequence due to successful amplification and hybridization.

[0185] Other detection methods known in the art may be used. For example, microfluidics (see, e.g., U.S. Patent Publication No. 2006 / 068398; U.S. Patent No. 6,544,734) provide methods and devices that can be used to separate and amplify DNA samples or molecules. Optical dyes can be used to detect and measure specific DNA molecules (see, e.g., WO / 05017181). Nanotube devices (see, e.g., WO / 06024023) that comprise an electronic sensor for the detection of DNA molecules or nanobeads that bind specific DNA molecules can then be detected.75 US_ACTIVE\129309744\V-1

[0186] DNA detection kits that are based on DNA amplification methods contain DNA primer molecules that hybridize specifically to a target DNA and amplify a diagnostic amplicon under the appropriate reaction conditions. The kit may provide an agarose gel-based detection method or any number of methods of detecting the diagnostic amplicon that are known in the art. DNA detection kits can be developed using the compositions disclosed herein and are useful for identification of corn event MON87429 DNA or DNA of a modified corn event MON87429 in a sample and can be applied to methods for breeding corn plants containing event MON87429 DNA or DNA of a modified corn event MON87429. A kit that contains DNA primers that are homologous or complementary to any portion of the corn genomic region as set forth in SEQ ID NO: 10 and to any portion of the inserted transgenic DNA as set forth in SEQ ID NO: 10 is an object of the invention. The DNA molecules can be used in DNA amplification methods (PCR) or as probes in polynucleic acid hybridization methods, i.e., southern analysis, northern analysis. Kits of the invention may optionally also comprise reagents or instructions for performing the detection or diagnostic reactions described herein.

[0187] Probes and primers as provided herein may have complete sequence identity with the target sequence, although primers and probes differing from the target sequence that retain the ability to hybridize preferentially to target sequences may be designed by conventional methods. In order for a nucleic acid molecule to serve as a primer or probe it needs only be sufficiently complementary in sequence to be able to form a stable double-stranded structure under the particular solvent and salt concentrations employed. Any conventional nucleic acid hybridization or amplification method can be used to identify the presence of transgenic DNA from corn event MON87429 in a sample.

[0188] Any number of methods well known to those skilled in the art can be used to isolate and manipulate a DNA molecule, or fragment thereof, disclosed herein, including DNA isolation or thermal amplification or PCR methods. Such DNA molecule or fragment may be inserted or placed into any suitable vector or plasmid or combined with other elements, sequences or fragments using molecular or recombinant techniques.

[0189] The DNA molecules and corresponding nucleotide sequences provided herein are therefore useful for, among other things, identifying corn event MON87429 or a modified corn event MON87429, detecting the presence of DNA derived from the transgenic corn event MON87429 or a modified corn event MON87429 in a sample, and monitoring samples for the presence and / or76 US_ACTIVE\129309744\V-1absence of corn event MON87429 or a modified corn event MON87429 or plant parts derived from corn plants comprising event MON87429 or a modified corn event MON87429.

[0190] Reference herein to “corn” generally is intended to include corn plants, corn plant cells, corn plant tissues, corn seeds, corn plant parts, corn progeny plants, and / or corn commodity products, depending on the context of its use herein, unless otherwise provided. The present disclosure provides corn plants, corn plant cells, corn plant tissues, corn seeds, corn plant parts (such as flowers, pollen, anther, silk, microspore, spike, cob, ovary, ovule, embryo, root tissue, stalk tissue, leaf tissue, and callus), corn progeny plants, and corn commodity products. These corn plants, corn plant cells, corn plant tissues, corn seeds, corn plant parts, corn progeny plants, and corn commodity products contain a detectable amount of a polynucleotide or DNA molecule or sequence comprising at least one junction sequence and / or heterologous insert sequence of corn event MON87429 or a modified corn event MON87429, such as a polynucleotide or DNA molecule or sequence having or comprising at least one of the sequences provided as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10.

[0191] Corn plants, corn plant cells, corn seeds, corn plant parts (such as flower, pollen, anther, silk, microspore, spike, cob, ovary, ovule, embryo, root tissue, stalk tissue, leaf tissue, callus), corn progeny plants derived from a transgenic corn plant containing event MON87429 DNA or DNA of a modified corn event MON87429 are provided. A representative sample of corn seed containing event MON87429 DNA has been deposited according to the Budapest Treaty with the American Type Culture Collection (ATCC®). The ATCC repository has assigned the Patent Deposit Designation PTA-124635 to the seed containing event MON87429 DNA.

[0192] A microorganism is provided. A microorganism is intended to include any microscopic cell or organism, whether prokaryote or eukaryote or otherwise, that contains DNA within a genome or chromosome or an extra-chromosomal DNA structure, such as a plasmid or vector, in such microscopic cell. The microorganism comprises a polynucleotide molecule having the nucleotide sequence of SEQ ID NO:9, or a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:9.77 US_ACTIVE\129309744\V-1An example of such a microorganism is an Agrobacterium cell. Another example of such a microorganism is an E. coli cell.

[0193] A plant cell is provided comprising a polynucleotide molecule as described herein. For example, a plant cell is provided having a nucleotide sequence present in its genome, wherein the nucleotide sequence is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, and a nucleic acid molecule comprising a polynucleotide having a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9.

[0194] Plant cells and microorganisms of the present disclosure are useful in many industrial applications, including but not limited to: (i) use as research tools for scientific inquiry or industrial research; (ii) use in culture for producing endogenous or recombinant carbohydrate, lipid, nucleic acid, enzymes or protein products or small molecules that may be used for subsequent scientific research or as industrial products; and (iii) use with modern plant tissue culture techniques to produce transgenic plants or plant tissue cultures that may then be used for agricultural research or production. The production and use of such transgenic plant cells utilize modern microbiological techniques and human intervention to produce a man-made, unique microorganism and plant cell. In this process, a recombinant DNA is inserted into a plant cell’s genome to create a transgenic plant cell that is separate and unique from naturally occurring plant cells. This transgenic plant cell can then be cultured much like bacteria and yeast cells using modern microbiology techniques and may exist in an undifferentiated, unicellular state. The transgenic plant cell’s new genetic composition and phenotype is a technical effect created by the integration of the heterologous DNA into the genome of the cell.

[0195] Provided are methods of using a plant cell, such as transgenic plant cells. These include (i) methods of producing transgenic cells by integrating a recombinant DNA into the genome of the cell and then using this cell to derive additional cells possessing the same heterologous DNA; (ii) methods of culturing cells that contain recombinant DNA using modern microbiology techniques; (iii) methods of producing and purifying endogenous or recombinant carbohydrate,78 US_ACTIVE\129309744\V-1lipid, nucleic acid, enzymes or protein products from cultured cells; and (iv) methods of using modern plant tissue culture techniques with transgenic plant cells to produce transgenic plants or transgenic plant tissue cultures.

[0196] Corn plants of the present disclosure may pass along the event MON87429 DNA or the modified event MON87429 DNA, including the transgene inserted in corn event MON87429 or the modified event MON87429, to progeny. As used herein, “progeny” includes any plant, plant cell, seed, and / or regenerable plant part containing the event MON87429 DNA derived from an ancestor plant and / or comprising a DNA molecule having at least one sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. Corn plants, progeny, and seeds may be homozygous or heterozygous for the transgene of event MON87429 or the modified event MON87429. Progeny may be grown from seeds produced by a corn event MON87429 or a modified corn event MON87429-containing plant and / or from seeds produced by a plant fertilized with pollen from a corn event MON87429 or a modified corn event MON87429-containing plant.

[0197] Methods for producing corn plants and seeds containing or comprising corn event MON87429 are provided. Corn plants may be bred using any method known in the art, for example, descriptions of breeding methods that are commonly used can be found in WR Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987). Corn plants or progeny plants containing or comprising corn event MON87429 may be self-pollinated (also known as “selfing”) to generate a true breeding line of corn plants, i.e., corn plants homozygous for the transgene and event MON87429. Selfing can result in progeny known as an “inbred” that can be used to produce corn inbred lines that are genetically uniform.

[0198] Alternatively, corn plants or progeny plants containing or comprising corn event MON87429 or a modified corn event MON87429 may be out-crossed or cross-pollinated (also known as “crossing”), e.g., bred with another plant having a different germplasm or genotype, to produce a varietal or hybrid seed or plant that may be homozygous or heterozygous for the transgene and event MON87429 or a modified event MON87429 depending on whether the other parental plant also comprises or contains the transgene and event MON87429 or a modified event MON87429. The other parental plant may be transgenic or non-transgenic for the same and / or79 US_ACTIVE\129309744\V-1different trait, transgene or event. A varietal or hybrid seed or plant of the invention may thus be derived by sexually crossing a first parent that lacks the specific and unique corn event MON87429 or a modified corn event MON87429 with a second parent comprising corn event MON87429 or a modified corn event MON87429, resulting in a hybrid plant or progeny plant containing or comprising the specific and unique corn event MON87429 or a modified corn event MON87429. Each parent can be a hybrid or an inbred / varietal plant, so long as a parent or progeny plant or seed of the cross has or comprises at least one copy of the corn event MON87429 or a modified corn event MON87429 and / or a DNA molecule having or comprising at least one sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10.

[0199] Sexually crossing one plant with another plant, i.e., cross-pollinating, may be accomplished or facilitated by human intervention, for example: by human hands or other mechanical means under human, computer or automated control collecting the pollen of one plant and contacting this pollen with the style or stigma of a second plant; by human hands and / or human actions or other mechanical means under human, computer or automated control removing, destroying, devitalizing or covering the stamen or anthers of a plant (e.g., by manual intervention or by application of a chemical gametocide) so that natural self-pollination is prevented and cross- pollination would have to take place in order for fertilization to occur; by human placement of pollinating insects in a position for “directed pollination” (e.g., by placing beehives in orchards or fields or by caging plants with pollinating insects); by human opening or removing of parts of the flower to allow for placement or contact of foreign pollen on the style or stigma; by selective placement of plants (e.g., intentionally planting plants in pollinating proximity); and / or by application of chemicals to precipitate flowering or to foster receptivity (of the stigma for pollen).

[0200] Two different transgenic plants may thus be crossed to produce hybrid offspring plants, plant parts and / or seeds that contain two independently segregating transgenes or events wherein at least one of those transgenes or events comprises or is contained within corn event MON87429 or a modified corn event MON87429. For example, transgenic plants comprising corn event MON87429 or a modified corn event MON87429 can be crossed with other transgenic corn plants to produce a plant having the characteristics of both transgenic parents. Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated, as is vegetative propagation. Descriptions of other breeding methods that are commonly used for different traits80 US_ACTIVE\129309744\V-1and crops are known in the art and can be found in one of several references, e.g., Fehr, in Breeding Methods for Cultivar Development, Wilcox J. ed., American Society of Agronomy, Madison WI (1987).

[0201] The corn progeny plants, plant parts or seeds may be analyzed using diagnostic methods as described herein to select for progeny plants, plant parts or seeds containing or comprising corn event MON87429 or a modified corn event MON87429. Alternatively or in addition, corn progeny plants, plant parts or seeds containing or comprising corn event MON87429 or a modified corn event MON87429 may be identified or selected by treating the progeny plants, plant parts or seeds with an effective amount of at least one herbicide selected from the group consisting of glufosinate, dicamba, 2,4-D, glyphosate, and combinations of any thereof.

[0202] Corn plants, progeny, seeds, cells and plant parts of the present disclosure may also contain one or more additional corn trait(s) or transgenic events, particularly those introduced by crossing a corn plant containing corn event MON87429 or a modified corn event MON87429 with another corn plant containing the additional trait(s) or transgenic events. Such trait(s) or transgenic events include, but are not limited to, increased insect resistance, herbicide tolerance, increased water use efficiency, increased yield performance, increased drought resistance, increased seed quality, improved nutritional quality, hybrid seed production, or disease or fungal resistance. Corn transgenic events are known to those of skill in the art. For example, a list of such traits is provided by the United States Department of Agriculture’s (USDA) Animal and Plant Health Inspection Service (APHIS) and can be found on their website: www.aphis.usda.gov. Two or more transgenic events may thus be combined in a progeny seed or plant by crossing two parent plants each comprising one or more transgenic events, collecting the progeny seed, and selecting for progeny seed or plants that contain the two or more transgenic events. These steps may be repeated until the desired combination of transgenic events in a progeny is achieved. Back-crossing to a parental plant and out-crossing with a non-transgenic plant are also contemplated and is vegetative propagation.

[0203] A plant part is provided that comprises event MON87429 or a modified corn event MON87429 and / or is derived from corn plants comprising event MON87429 or a modified corn event MON87429. As used herein, a “plant part” refers to any part of a plant which may comprise event MON87429 or a modified corn event MON87429 and / or material derived from a corn plant81 US_ACTIVE\129309744\V-1comprising event MON87429 or a modified corn event MON87429. Plant parts include, but are not limited to, plant tissue, flower, anther, silk, pollen, microspore, spike, cob, ovary, ovule, embryo, root tissue, stem or stalk tissue, leaf tissue and callus. Plant parts may be viable, nonviable, regenerable, and / or non-regenerable.

[0204] A commodity product is provided that is derived from one or more corn plants, plant parts, seeds and / or plant tissues comprising event MON87429 or a modified corn event MON87429 and that contains a detectable amount of a nucleic acid or DNA molecule, segment or sequence specific for event MON87429 or a modified corn event MON87429. As used herein, a “commodity product” refers to any composition or product comprising material derived from one or more corn plants, whole or processed corn seed, one or more plant cells, and / or one or more plant parts containing or comprising the corn event MON87429 DNA or a modified corn event MON87429. Nonviable commodity products include, but are not limited to, nonviable seeds, whole or processed seeds, seed parts, and plant parts; food for human consumption or animal feed comprising corn, corn oil, corn meal, corn flour, corn flakes, corn bran, grits, corn starch, pasta made with corn, corn biomass, fiber, sweetener such as high fructose corn syrup (HFCS), glucose and dextrose, beverage alcohol, brewer grits for beer production, and fuel products produced using corn and corn parts, corn pollen, corn plastic, dried distillers grains (DDGs), and bio-degradable packing materials. Viable commodity products include, but are not limited to, seeds, plants, and plant cells. The corn plants comprising event MON87429 or a modified corn event MON87429 can thus be used to manufacture any commodity product typically acquired from corn. Any such commodity product that is derived from corn plants comprising event MON87429 or a modified corn event MON87429 may contain at least a detectable amount of the specific and unique DNA corresponding to corn event MON87429 or a modified corn event MON87429, and specifically may contain a detectable amount of a polynucleotide or DNA molecule having or comprising at least one sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a polynucleotide having a sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical to the full length of SEQ ID NO:10 or the full length of SEQ ID NO:9. Detection of one or more of these polynucleotide or DNA sequences in a sample may be82 US_ACTIVE\129309744\V-1used to determine or diagnose that the sample is taken from a corn plant, corn plant part, corn plant tissue, corn plant cell, and / or corn plant product, such as a corn commodity product, comprising event MON87429 or a modified corn event MON87429, or to determine the content or source of a corn plant, corn plant part, corn plant tissue, corn plant cell, and / or corn plant product, such as a corn commodity product. Any standard method of detection for nucleotide molecules may be used, including methods of detection disclosed herein. A commodity product is within the scope of the present disclosure if there is any detectable amount of a DNA molecule disclosed herein contained or comprised in the commodity product.

[0205] The corn plants, corn plant cells, corn seeds, corn plant parts (such as plant tissue, flower, anther, silk, pollen, microspore, spike, cob, ovary, ovule, embryo, root tissue, stem or stalk tissue, leaf tissue and callus), corn progeny plants, and commodity products of the present disclosure are therefore, useful for, among other things, growing plants for the purpose of producing seed and / or plant parts comprising corn event MON87429 or a modified corn event MON87429 for agricultural purposes, producing progeny comprising corn event MON87429 or a modified corn event MON87429 for plant breeding and research purposes, use with microbiological techniques for industrial and research applications, and sale to consumers.

[0206] Methods for producing an herbicide tolerant corn plant comprising the DNA sequences specific and unique to event MON87429 or a modified corn event MON87429 of the present disclosure are provided. Transgenic plants used in these methods may be homozygous or heterozygous for the transgenes. Progeny plants produced by these methods may be varietal or hybrid plants; may be grown from seeds produced by corn event MON87429 or a modified corn event MON87429-containing plants and / or from seeds produced by a plant fertilized with pollen from a corn event MON87429 or a modified corn event MON87429-containing plant; and may be homozygous or heterozygous for the transgenes. Progeny plants may be subsequently self- pollinated to generate a true breeding line of plants, i.e., plants homozygous for the transgene, or alternatively may be out-crossed, e.g., bred with another unrelated plant, to produce a varietal or a hybrid seed or plant.

[0207] Methods of detecting the presence of DNA derived from a corn cell, corn tissue, corn seed, corn plant or commodity product comprising corn event MON87429 or a modified corn event MON87429 in a sample are provided. One method consists of (i) extracting a DNA sample from at least one corn cell, corn tissue, corn seed, corn plant or commodity product; (ii) contacting the83 US_ACTIVE\129309744\V-1DNA sample with at least one primer that is capable of producing DNA sequence specific to event MON87429 or modified event MON87429 DNA under conditions appropriate for DNA sequencing; (iii) performing a DNA sequencing reaction; and then (iv) confirming that the nucleotide sequence comprises a nucleotide sequence specific for event MON87429 or modified event MON87429, of the construct comprised therein, such as one selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10.

[0208] Another method comprises (i) extracting a DNA sample from at least one corn cell, corn tissue, corn seed, corn plant or commodity product; (ii) contacting the DNA sample with a primer pair that is capable of producing an amplicon from event MON87429 or modified event MON87429 DNA under conditions appropriate for DNA amplification; (iii) performing a DNA amplification reaction; and then (iv) detecting the amplicon molecule and / or confirming that the nucleotide sequence of the amplicon comprises a nucleotide sequence specific for event MON87429 or modified event MON87429, such as one selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. The amplicon should be one that is specific for event MON87429, such as an amplicon that comprises SEQ ID NO:1, or SEQ ID NO:2, or SEQ ID NO:3, or SEQ ID NO:4, or SEQ ID NO:5, or SEQ ID NO:6, or SEQ ID NO:7, or SEQ ID NO:8, or SEQ ID NO:9, or SEQ ID NO:10. The detection of a nucleotide sequence specific for event MON87429 or modified event MON87429 in the amplicon is determinative and / or diagnostic for the presence of the corn event MON87429 or modified corn event MON87429-specific DNA in the sample. An example of a primer pair that is capable of producing an amplicon from event MON87429 DNA or modified event MON87429 DNA under conditions appropriate for DNA amplification is provided as SEQ ID NO:11 and SEQ ID NO:12. Other primer pairs may be readily designed by one of skill in the art and would produce an amplicon comprising SEQ ID NO:1, or SEQ ID NO:2, or SEQ ID NO:3, or SEQ ID NO:4, or SEQ ID NO:5, or SEQ ID NO:6, or SEQ ID NO:7, or SEQ ID NO:8 wherein such a primer pair comprises at least one primer within the genomic region flanking the insert and a second primer within the insert.

[0209] Another method of detecting the presence of DNA derived from a corn cell, corn tissue, corn seed, corn plant or commodity product comprising corn event MON87429 or a modified corn event MON87429 in a sample consists of (i) extracting a DNA sample from at least one corn cell,84 US_ACTIVE\129309744\V-1corn tissue, corn seed, or corn plant; (ii) contacting the DNA sample with a DNA probe specific for event MON87429 DNA or DNA of a modified corn event MON87429; (iii) allowing the probe and the DNA sample to hybridize under stringent hybridization conditions; and then (iv) detecting hybridization between the probe and the target DNA sample. An example of the sequence of a DNA probe that is specific for event MON87429 or a modified event MON87429 is provided as SEQ ID NO:13. Other probes may be readily designed by one of skill in the art and would comprise at least one fragment of genomic DNA flanking the insert and at least one fragment of insert DNA such as the sequence provided in SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10. Detection of probe hybridization to the DNA sample is diagnostic for the presence of corn event MON87429 or modified corn event MON87429-specific DNA in the sample. Absence of hybridization is alternatively diagnostic of the absence of corn event MON87429 or modified corn event MON87429-specific DNA in the sample.

[0210] DNA detection kits are provided that are useful for the identification of corn event MON87429 DNA or DNA of a modified corn event MON87429 in a sample and can also be applied to methods for breeding corn plants containing the appropriate event DNA. Such kits contain DNA primers and / or probes comprising fragments of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. One example of such a kit comprises at least one DNA molecule of sufficient length of continuous nucleotides of SEQ ID NO:10 to function as a DNA probe useful for detecting the presence and / or absence of DNA derived from transgenic corn plants comprising event MON87429 or a modified event MON87429 in a sample. The DNA derived from transgenic corn plants comprising event MON87429 or a modified event MON87429 would comprise a DNA molecule having at least one sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10. A DNA molecule sufficient for use as a DNA probe that is useful for determining, detecting, or diagnosing the presence and / or absence of corn event MON87429 DNA or a modified event MON87429 in a sample is provided as SEQ ID NO:13. Other probes may be readily designed by one of skill in the art and should comprise at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 28, at least 29, at least 30, at least 31, at least 32, at least 33, at least 34, at least85 US_ACTIVE\129309744\V-135, at least 36, at least 37, at least 38, at least 39, or at least 40 contiguous nucleotides of SEQ ID NO:10 and be sufficiently unique to corn event MON87429 DNA or a modified event MON87429 in order to identify DNA derived from the event.

[0211] Another type of kit comprises a primer pair useful for producing an amplicon useful for detecting the presence and / or absence of DNA derived from transgenic corn event MON87429 or a modified event MON87429 in a sample. Such a kit would employ a method comprising contacting a target DNA sample with a primer pair as described herein, then performing a nucleic acid amplification reaction sufficient to produce an amplicon comprising a DNA molecule having at least one sequence selected from SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10 and then detecting the presence and / or absence of the amplicon. Such a method may also include sequencing the amplicon or a fragment thereof, which would be determinative of, i.e., diagnostic for, the presence of the corn event MON87429 or modified event MON87429-specific DNA in the target DNA sample. Other primer pairs may be readily designed by one of skill in the art and should comprise at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 26, at least 27, at least 28, at least 29, or at least 30 contiguous nucleotides of sequences provided in, but not limited to SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, and be sufficiently unique to corn event MON87429 DNA or modified event MON87429 DNA in order to identify DNA derived from the event.

[0212] Also provided are protein detection kits for detecting the presence of corn event MON87429 or a modified event MON87429 in a sample. One example of such a kit is a kit comprising antibodies specific for the PAT, DMO, FT and EPSPS proteins encoded by corn event MON87429 or a modified event MON87429. Detecting binding of the antibodies to the proteins encoded by corn event MON87429 or a modified event MON87429 in a sample is diagnostic for the presence of corn event MON87429 or the modified event MON87429 in the sample.

[0213] The kits and detection methods of the present disclosure are useful for, among other things, identifying corn event MON87429 or a modified event MON87429, selecting plant varieties or hybrids comprising corn event MON87429 or a modified event MON87429, detecting the presence of DNA derived from a transgenic corn plant comprising event MON87429 or a modified event MON87429 in a sample, and monitoring samples for the presence and / or absence of corn86 US_ACTIVE\129309744\V-1plants comprising event MON87429 or a modified event MON87429, or plant parts derived from corn plants comprising event MON87429 or a modified event MON87429.

[0214] The sequences of the heterologous DNA insert, junction sequences, or flanking sequence from corn event MON87429 or a modified event MON87429 can be verified (and corrected if necessary) by amplifying such sequences from the event using primers derived from the sequences provided herein followed by standard DNA sequencing of the amplicon or of the cloned DNA.

[0215] Methods of detecting the zygosity of the transgene allele of DNA derived from a corn cell, corn tissue, corn seed, or corn plant comprising corn event MON87429 or a modified event MON87429 in a sample are provided. One method consists of (i) extracting a DNA sample from at least one corn cell, corn tissue, corn seed, or corn plant; (ii) contacting the DNA sample with a primer pair that is capable of producing a first amplicon diagnostic for event MON87429 or a modified event MON87429; (iii) contacting the DNA sample with a primer pair that is capable of producing a second amplicon diagnostic for native corn genomic DNA not comprising event MON87429 or a modified event MON87429; (iv) performing a DNA amplification reaction; and then (v) detecting the amplicons, wherein the presence of only the first amplicon is diagnostic of a homozygous event MON87429 DNA or DNA of the modified event MON87429 in the sample, and the presence of both the first amplicon and the second amplicon is diagnostic of a corn plant heterozygous for event MON87429 or the modified event MON87429 allele. An exemplary primer pair is presented as SEQ ID NO:11 and SEQ ID NO:12 which produce an amplicon diagnostic for event MON87429. A set of probes can also be incorporated into such an amplification method to be used in a real-time PCR format using the primer pair sets described above. An exemplary probe is presented as SEQ ID NO:13 (diagnostic for the amplicon for the event MON87429).

[0216] Another method for determining zygosity consists of (i) extracting a DNA sample from at least one corn cell, corn tissue, corn seed, or corn plant; (ii) contacting the DNA sample with a probe set which contains at least a first probe that specifically hybridizes to event MON87429 DNA or DNA of a modified event MON87429 and at least a second probe that specifically hybridizes to corn genomic DNA that was disrupted by insertion of the heterologous DNA of event MON87429 and does not hybridize to event MON87429 DNA or DNA of a modified event MON87429; (iii) hybridizing the probe set with the sample under stringent hybridization conditions, wherein detecting hybridization of only the first probe under the hybridization87 US_ACTIVE\129309744\V-1conditions is diagnostic for a homozygous allele of event MON87429 DNA or DNA of the modified event MON87429 in the sample; and wherein detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a heterozygous allele of event MON87429 or the modified event MON87429 in a DNA sample.

[0217] Yet another method for determining zygosity consists of (i) extracting a DNA sample from at least one corn cell, corn tissue, corn seed, or corn plant; (ii) contacting the DNA sample with a primer pair that is capable of producing an amplicon of one of the transgenes coding sequences encoding PAT, DMO, FT and EPSPS; (iii) contacting the DNA sample with a primer pair that is capable of producing an amplicon of an internal standard known to be single-copy and homozygous in the corn plant; (iv) contacting the DNA sample with a probe set which contains at least a first probe that specifically hybridizes to one of the transgene coding sequences encoding PAT, DMO, FT and EPSPS, and at least a second probe that specifically hybridizes to the internal standard genomic DNA known to be single-copy and homozygous in the corn plant; (v) performing a DNA amplification reaction using real-time PCR and determining the cycle thresholds (Ct values) of the amplicon corresponding to the toxin coding sequence and the single- copy, homozygous internal standard; (vi) calculating the difference (∆Ct) between the Ct value of the single-copy, homozygous internal standard amplicon and the Ct value of the toxin coding sequence amplicon; and (vii) determining zygosity, wherein a ∆Ct of around zero (0) indicates homozygosity of the inserted T-DNA and a ∆Ct of around one (1) indicates heterozygosity of the inserted T-DNA. Heterozygous and homozygous events are differentiated by a ∆Ct value unit of approximately one (1). Given the normal variability observed in real-time PCR due to multiple factors such as amplification efficiency and ideal annealing temperatures, the range of “about one (1)” is defined as a ∆Ct of 0.75 to 1.25. Primer pairs and probes for the above method for determining zygosity can amplify and detect amplicons from the 3´ junction sequence.

[0218] A transgenic corn plant or plant part, one or more transgenic corn plants or plant parts or a plurality transgenic corn plants or plant parts as provided herein, or an agricultural field or soil in which a transgenic corn plant or plant part, one or more transgenic corn plants or plant parts or a plurality of transgenic corn plants or plant parts as provided herein are planted or grown, can be treated with an agricultural composition comprising one or more active ingredients or other agents, such as, for example and without limitation, an herbicide or one or more herbicides, a fungicide or one or more fungicides, an insecticide or one or more insecticides, a plant growth regulator or plant88 US_ACTIVE\129309744\V-1stimulant or one or more plant growth regulators and / or plant stimulants, and / or a safener or one or more safeners. Provided below are lists of possible or representative compounds for each of these types of actives or agents, and an agricultural composition may comprise one or any combination or multiplicity of these actives, agents or compounds. Such an agricultural composition may be applied, for example, as a foliar, soil or in-furrow treatment, as a pre- emergent, pre-sowing and / or post-emergent treatment, and / or in some cases, may be applied to a transgenic plant part or seed provided herein.

[0219] An agricultural composition may be formulated according to its intended use and application. The appropriate formulation of the agricultural composition may be chosen to have different physicochemical parameters, components and stabilities of the respective compound(s). Possible types of formulations for an agricultural composition can include, for example: wettable powders (WP), water-soluble powders (SP), water-soluble concentrates, emulsifiable concentrates (EC), emulsions (EW), such as oil-in-water and water-in-oil emulsions, sprayable solutions, suspension concentrates (SC), dispersions based on oil or water, oil-miscible solutions, capsule suspensions (CS), dusting products (DP), dressings, granules for scattering and soil application, granules (GR) in the form of microgranules, spray granules, absorption and adsorption granules, water-dispersible granules (WG), water-soluble granules (SG), ULV formulations, microcapsules and waxes. If appropriate, some agricultural compositions of a pesticidal compound or one or more pesticidal compounds might be formulated and used as a seed coating applied to a plant part or seed as provided herein.

[0220] Plants, progeny, plant parts, plant seeds, plant tissues, and plant cells may contain or comprise one or more additional desirable trait(s). Such desirable traits may be transgenic traits, native traits, or traits produced by other methods, such as genome editing, base editing, prime editing or other conventional mutagenesis methods. Such desirable trait(s) may provide an agronomic, agricultural or commodity benefit to a plant, plant part, plant seed or plant product. Desirable traits may be combined with corn event MON87429 or a modified corn event MON87429 by, for example, crossing a corn plant comprising corn event MON87429 or a modified corn event MON87429 with another corn plant containing the additional trait(s). Alternatively, a trait may be created by mutagenesis, editing or site-directed integration of or into a plant, plant part or plant cell comprising corn event MON87429 or a modified corn event MON87429. Such traits may include, but are not limited to, increased insect resistance, increased89 US_ACTIVE\129309744\V-1water use efficiency, increased nitrogen use efficiency, increased yield performance, increased drought resistance, increased disease resistance, increased seed quality, improved nutritional quality, hybrid seed production, and / or increase herbicide tolerance, in which the trait is measured with respect to a corn plant lacking such transgenic trait. For example, the MON87429 event or a modified MON87429 event could be stacked by breeding or introgression with another event(s), or a combination of events, known in the art including, but not limited to: • MON00603 (also known as NK603 or MON603, Roundup Ready™ 2 Maize for herbicide tolerance; deposited as ATCC PTA-2478, described in US Patent No. 6,825,400, and USDA APHIS Petition No.00-011-01p (Extension of 97-099-01p), the entire contents and disclosure of each of which are incorporated herein by reference), • MON89034 (also known as MON-89034-3 and YieldGard™ VT Pro™ for insect resistance; deposited as ATCC PTA-7455, described in PCT Publication No. WO2007 / 140256, US Patent No. 8,062,840, and USDA APHIS Petition No. 06-298-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON88017 (also known as MON-88017-3 and YieldGard™ VT™ Rootworm™ RR2 for herbicide tolerance and insect resistance; deposited as ATCC PTA-5582, described in US Patent No. 8,212,113 and PCT Publication No. WO2005 / 059103, and USDA APHIS Petition No. 04-125-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON87427 (also known as MON-87427-7 and Roundup Ready™ Maize for herbicide tolerance; deposited as ATCC PTA-7899, described in US Patent No. 8,618,358 and PCT Publication No. WO2011 / 062904, and USDA APHIS Petition No. 10-281-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON87411 (for insect resistance; deposited as ATCC PTA-12669, described in US Patent No. 10,316,330 and PCT Publication No. WO2013 / 169923, USDA APHIS Petition No. 13-290-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON87460 (also known as MON-87460-4 and Genuity® DroughtGard™ for abiotic stress tolerance; deposited as ATCC PTA-8910, described in PCT Publication No.90 US_ACTIVE\129309744\V-1WO2009 / 111263 and US Patent No. 8,450,561, and USDA APHIS Petition No. 09-055- 01p. the entire contents and disclosure of each of which are incorporated herein by reference), • MON87419 (for herbicide tolerance; deposited as ATCC PTA-120860, described in US Patent No.11,098,321 and PCT Publication WO2015 / 142571, and USDA APHIS Petition No.15-113-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON95379 (for insect resistance; deposited as ATCC PTA-125027, described in PCT Publication WO2020 / 028172) and US Patent No. 11,198,887, and UDSA APHIS Petition No.20-205-01p, the entire contents and disclosure of each of which are incorporated herein by reference. • MON810 (also known as MON-00810-6, YieldGard™ and MaizeGard™ for insect resistance; described in US Patent No. 6,713,259, and USDA APHIS Petition No.96-017- 01p (extension of 95-093-01p), the entire contents and disclosure of each of which are incorporated herein by reference), • MON00021 (also known as GA21, Roundup Ready™ Maize and Agrisure™GT for herbicide tolerance; deposited as ATCC PTA-209033 and described in US Patent No. 6,762,344 and PCT Publication No. WO1998 / 044140, USDA APHIS Petition No.97-099- 01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MON832 (also known as Roundup Ready™ Maize for herbicide tolerance), • MON863 (also known as MON-00863-5, YieldGard™, Rootworm RW and MaxGard™ for insect resistance; deposited as ATCC PTA-2605 and described in PCT Publication No. WO2004 / 011601 and US Patent No. 7,705216, USDA APHIS Petition No. 01-137-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • Zm_CSM63715 (also known as MON80616 for PPO herbicide tolerance; deposited as ATCC PTA-127361, and described in USDA Regulatory Status Review No.23-208-01rsr, the entire contents and disclosure of each of which are incorporated herein by reference),91 US_ACTIVE\129309744\V-1• ZM_BCS216090 (also known as Short Stature Maize, MON94804 and Preceon™ for altered plant architecture, deposited as ATCC PTA-127050, described in US Patent Application Publication No. 2023 / 0279508 and USDA Regulatory Status Review No. 22- 152-01rsr, the entire contents and disclosure of which are incorporated herein by reference), • AGV-PY203-4 (also known as GraINzyme Phytase and PY203 for modified product quality; described in USDA-APHIS Petition 19-176-01p, the entire content and disclosure of which are incorporated herein by reference), • ACS-ZM004-3 (also known as CBH-351, Starlink™ Maize for herbicide tolerance and insect resistance; described in USDA APHIS Petition No. 97-265-01p, the entire content and disclosure of which are incorporated herein by reference), • ACS-ZM001-9 (also known as MS3, InVigor™ Maize for pollination control system, described in USDA APHIS Petition No. 95-228-01p, the entire content and disclosure of which are incorporated herein by reference), • ACS-ZM005-4 (also known as MS6, InVigor™ Maize for pollination control system; described in USDA APHIS Petition No. 98-349-01p (extension of 95-228-01p), the entire content and disclosure of which are incorporated herein by reference), • ACS-ZM002-1 (also known as T14 and Liberty Link™ Maize for herbicide tolerance, described in USDA APHIS Petition No. 94-357-01p, the entire contents and disclosure of which are incorporated herein by reference), • ACS-ZM003-2 (also known as T25 and GAT-ZM1 Liberty Link™ Maize for herbicide tolerance; described in US Patent No. 6,951,929, and USDA APHIS Petition No. 94-357- 01p, the entire contents and disclosure of each of which are incorporated herein by reference), • DAS-40278-9 (also known as DAS40278 and Enlist™ Maize for herbicide tolerance; deposited as ATCC No. PTA-10244 and described in US Patent No. 11,098,322 and PCT Publication No. WO2011 / 022469, and USDA APHIS Petition No. 09-233-01p, the entire contents and disclosure of each of which are incorporated herein by reference),92 US_ACTIVE\129309744\V-1• DAS-01507-1 (also known as TC1507, Herculex™ I and Herculex™ CB for herbicide tolerance and insect resistance; described in US Patent No.7,288,643 and PCT Publication No WO2004 / 099447, and USDA APHIS Petition No. 00-136-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • DAS-59122-7 (also known as Herculex™ RW for insect resistance; described in US Patent No. 7,875,429 and USDA APHIS Petition No. 05-353-01p, the entire content and disclosure of each of which are incorporated herein by reference), • DKB-89614-9 (also known as DBT418 and Bt Xtra™ Maize for insect resistance; described in USDA APHIS Petition No. 96-291-01p, the entire content and disclosure of which are incorporated herein by reference), • DP-004114-3 (Also known as 4114 for herbicide tolerance and insect resistance; described in USDA APHIS Petition No. 11-244-01p, the entire content and disclosure of which are incorporated herein by reference), • DP-32138-1 (also known as 32138 and 32138 SPT maintainer for pollination control system; deposited as ATCC PTA-9158, described in US Patent No. 8,257,930, PCT Publication No. WO2009 / 103049, and USDA APHIS Petition No. 08-338-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • DP-098140-6 (also known as Optimum™ GAT™ for herbicide tolerance; deposited as ATCC PTA-8296, and described in US Patent No. 7,897,846 and PCT Publication No. WO2008 / 112019, and USDA APHIS Petition No. 07-152-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MIR162 (also known as SYN-IR162-4, Agrisure™ Viptera for insect resistance; deposited as ATCC PTA-6188, described in US Patent No. 8,232,456, PCT Publication No. WO2007 / 142840, and USDA APHIS Petition No. 07-253-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • MIR604 (also known as SYN-IR604-5 and Agrisure™ RW for insect resistance, described in US Patent Application Publication No. 7,361,813 and PCT Publication No.93 US_ACTIVE\129309744\V-1WO2005 / 103301, and USDA APHIS Petition No. 04-362-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • REN-00038-3 (also known as LY038 and Mavera™ Maize for modified product quality; deposited as ATCC PTA-5623, described in PCT Publication No. WO2005 / 061720 and US Patent No. 7,615,621, and USDA APHIS Petition No. 04-229-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • SYN-E3272-5 (also known as 3272 and Enogen™ for modified product quality, described in US Patent No. 7,635,799 and PCT Application Publication No. WO2006 / 098952, and USDA APHIS Petion No.05-280-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • SYN-05307-1 (also known as 5307 and Agrisure® Duracade™ for insect resistance; deposited as ATCC PTA-9561 and described in PCT Publication No. WO2010 / 077816 and US Patent No. 10,100,371, and USDA APHIS Petition No. 10-336-01p, the entire contents and disclosure of each of which are incorporated herein by reference), • Bt11 (also known as SYN-BT011-1 and Agrisure™ CB / LL for insect resistance; described in USDA APHIS Petition No. 95-195-01p, the entire content and disclosure of which are incorporated herein by reference), • SYN-EV176-9 (also known as Bt176, 176, NaturGard KnockOut™ and Maximizer™ for insect resistance; described in USDA APHIS Petition No. 94-319-01p, the entire content and disclosure of which are incorporated herein by reference), • MON89034 x DAS-01507-1 x MON603 (also known as Power Core™ for herbicide tolerance and insect resistance), • MON89034 x DAS-01507-1 x MON603 x MIR162 x DAS-40278-9 (also known as Power Core™ x MIR162 x Enlist™ for herbicide tolerance and insect resistance), • DAS-01507-1 x DAS-59122-7 (also known as Herculex XTRA™ for herbicide tolerance and insect resistance),94 US_ACTIVE\129309744\V-1• DAS-01507-1 x DAS-59122-7 x MON603 (also known as Herculex XTRA™ RR for herbicide tolerance and insect resistance), • DAS-01507-1 x MON603 (also known as Herculex™ I RR for herbicide tolerance and insect resistance), • DAS-59122-7 x MON603 (also known as Herculex™ RW Roundup Ready™ 2 for herbicide tolerance and insect resistance), • DP-004114-3 x MON603 (also known as Roundup Ready 2™), • DP-004114-3 x MON810 x MON603 (also known as Roundup Ready 2™ Yieldgard™), • DP-004114-3 x MIR604 x MON603 (also known as Roundup Ready 2 Agrisure RW™), • DP-004114-3 x MON810 (also known as Yieldgard™), • DP-004114-3 x MON810 x MIR604 (also known as Agrisure RW™ Yieldgard™), • DP-004114-3 x DAS-40278-9 (also known as Enlist™), • DP-004114-3 x MON87411 x DAS-40278-9 (also known as Enlist™), • DP-004114-3 x MON89034 (also known as Yieldgard VT Pro™), • DP-004114-3 x MON89034 x DAS-40278-9 (also known as Enlist™ Yieldgard VT Pro™), • DP-004114-3 x MON89034 x MON87411 (also known as Yieldgard VT Pro™), • DP-004114-3 x MON89034 x MON87411 x DAS-40278-9 (also known as Enlist™ Yieldgard VT Pro™), • DAS-01507-1 × DAS-59122-7 × MON00810 × MIR604 x MON603 (also known as Optimum™ Intrasect Xtreme for herbicide tolerance and insect resistance), • DAS-01507-1 x DAS-59122-7 x MON810 x MON603 (also known as Optimum™ Intrasect XTRA for herbicide tolerance and insect resistance), • DAS-01507-1 x MIR604 x MON603 (also known as Optimum™ TRIsect for herbicide tolerance and insect resistance),95 US_ACTIVE\129309744\V-1• DAS-01507-1 x MON810 x MON603 (also known as Optimum™ Intrasect for herbicide tolerance and insect resistance), • MON00021 x MON810 (also known as Roundup Ready™ YieldGard™ Maize for herbicide tolerance and insect resistance), • MON810 x MON88017 (also known as YieldGard™ VT Triple for herbicide tolerance and insect resistance), • MON863 x MON810 (also known as YieldGard™ Plus for insect resistance), • MON603 x MON810 x MON863 (also known as YieldGard™ Plus with RR for herbicide tolerance and insect resistance), • MON863 x MON603 (also known as YieldGard™ RW + RR for herbicide tolerance and insect resistance), • MON87427 x MON89034 x DAS-01507-1 x MON87411 x DAS-59122-7 x DAS-40278- 9 (also known as SmartStax™ Pro x Enlist™ for herbicide tolerance and insect resistance), • MON89034 x MON88017 (also known as Genuity® VT Triple Pro™ for herbicide tolerance and insect resistance), • MON89034 x MON603 (also known as Genuity® VT Double Pro™ for herbicide tolerance and insect resistance), • MON89034 x DAS-01507-1 x MON88017 x DAS-59122-7 (also known as Genuity® SmartStax™ for herbicide tolerance and insect resistance), • MON89034 x DAS-01507-1 x MON603 (also known as Power Core™ for herbicide tolerance and insect resistance), • MON603 x MON810 (also known as YieldGard™ CB + RR for herbicide tolerance and insect resistance), • MON603 x ACS-ZM003-2 x DAS40278 (also known as Roundup Ready™ Liberty Link™ Enlist™ Maize),96 US_ACTIVE\129309744\V-1• MON603 x ACS-ZM003-2 (also known as Roundup Ready™ Liberty Link™ Maize for herbicide tolerance), • ACS-ZM003-2 x MON810 (also known as Liberty Link™ Yieldgard™ Maize for herbicide tolerance and insect resistance), • REN-00038-3 x MON810 (also known as Mavera™ YieldGard™ Maize for insect resistance and modified product quality), • SYN-05307-1 x MIR604 x SYN-BT011-1 x DAS-01507-1 x MON00021 (also known as Agrisure® Duracade™ 5122 for herbicide tolerance and insect resistance), • SYN-05307-1 x MIR604 x SYN-BT011-1 x DAS-01507-1 x MON00021 x MIR162 (also known as Agrisure® Duracade™ 5222 for herbicide tolerance and insect resistance), • SYN-BT011-1 x DAS-59122-7 x MIR604 x DAS-01507-1 x MON00021 (also known as Agrisure® 3122 for herbicide tolerance and insect resistance), • SYN-BT011-1 x MON00021 (also known as Agrisure® GT / CB / LL for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 (also known as Agrisure® Viptera™ 2100 for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 MON603 (also known as Agrisure® Viptera™ 2100 Roundup Ready™ 2 Maize for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 x MON00021 (also known as Agrisure® Viptera™ 3110 for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 x MIR604 (also known as Agrisure® Viptera™ 3100 for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 x DAS-01507-1 x MON00021 (also known as Agrisure® Viptera™ 3220 for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR604 (also known as Agrisure® CB / LL / RW for herbicide tolerance and insect resistance),97 US_ACTIVE\129309744\V-1• SYN-BT011-1 x MIR604 x MON00021 (also known as Agrisure® Viptera™ 3000GT for herbicide tolerance and insect resistance), • SYN-BT011-1 x MIR162 x MIR604 x MON00021 (also known as Agrisure® Viptera™ 3111, Agrisure® Viptera™ 4 for herbicide tolerance and insect resistance), and / or • MIR604 x MON00021 (also known as Agrisure® GT / RW for herbicide tolerance and insect resistance). DEPOSIT INFORMATION

[0221] A deposit of a representative sample of corn seed containing event MON87429 was made on January 12, 2018, according to the Budapest Treaty with the American Type Culture Collection (ATCC) having an address at 10801 University Boulevard, Manassas, Virginia USA, Zip Code 20110. The deposit was accepted and assigned ATCC Accession No. PTA-124635. Access to the deposits will be available during the pendency of the application to the Commissioner of Patents and Trademarks and persons determined by the Commissioner to be entitled thereto upon request. Upon issuance of the patent, all restrictions upon availability to the public will be irrevocably removed. The deposit will be maintained in the depository for a period of thirty (30) years, or five (5) years after the last request, or for the effective life of the patent, whichever is longer, and will be replaced as necessary during that period. EXAMPLES

[0222] The following Examples are included to more fully describe the invention, Examples 1-6 describe the creation, testing, and detection of corn event MON87429, and Examples 7 and 8 describe illustrative modifications of corn event MON87429. Corn event MON87429 resulted from the construction and testing of 35 constructs, the production of over fifteen thousand unique events, and the analysis of hundreds of thousands of individual plants over seven years through the rigorous molecular, agronomic, and field testing required for the creation and ultimate selection of corn event MON87429.

[0223] Those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments that are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.98 US_ACTIVE\129309744\V-1Example 1: Expression Cassette Testing, Construct Design, Plant Testing and R0 Plant Testing

[0224] This example describes the design and testing in maize plants of thirty-five different constructs. Each construct contained four expression cassettes, each expression cassette used for expressing a different transgene. This testing was done to select the best construct to use for expressing all four transgenes in maize. Each construct had a unique configuration, varying by expression cassette orientation and expression elements.

[0225] Various individual expression cassettes with different expression element combinations and transgenes were designed, cloned into plant transformation vectors, and tested for trait efficacy in maize plants to create a pool of the best individual expression cassettes. Using this pool of individual expression cassettes, 35 different constructs were designed so that each contained four expression cassettes (each expression cassette having the transgene for PAT, DMO, FT or CP4- EPSPS) and could be used to test the four-way combination of the different expression cassettes. The expression cassette combinations varied by expression elements, protein coding sequence, and orientation. This resulted in the testing of two PAT expression cassettes, six DMO expression cassettes, five FT expression cassettes, and eighteen CP4-EPSPS expression cassettes.

[0226] The 35 four-expression cassette constructs were cloned into plant transformation vectors, and these vectors were used for Agrobacterium-mediated transformation of LH244 maize immature embryos using methods known in the art to produce 15,326 unique transformation events, each made by a random insertion of the transgene insert into the maize genome. R0 plants were then regenerated from the transgenic cells, and rooted plants with normal phenotypic characteristics were transferred to soil for growth and further assessment.

[0227] The 15,326 R0 plants were analyzed for having a single copy of the transgenic insert and absence of vector backbone sequence. Plants with a single copy of the insert were advanced for herbicide tolerance efficacy testing. Single copy R0 plants were assessed in the greenhouse for tolerance to quizalofop (0.16 lb ai / ac of Assure II® herbicide) followed by a tank mix of glufosinate (0.98 lb ae / ac of Ignite® 280 herbicide), dicamba (2.0 lb ae / ac of Clarity® herbicide), or 2,4-D (2.0 lb ae / ac 2,4-D Amine 4® herbicide) (or a combination of any of these) sprayed at the V1 / V2 growth stage. Plants that showed >30% injury were discarded.

[0228] From the initial 15,326 unique transformation events produced using the 35 transformation vectors, 1,945 unique events were selected after analyzing the copy number and herbicide spray99 US_ACTIVE\129309744\V-1data. Data are provided in Table 2. The R0 plants for the selected events were self-pollinated to produce R1 seed or crossed to produce F1 seed that was advanced to first season field trials. Table 2. First season filed trial inbred efficacy Construct # R0 Events # Events Advanced HT4-1 515 39US_ACTIVE\129309744\V-1Construct # R0 Events # Events Advanced HT4-51 132 9Example 2: First Season Field Trials

[0229] Field trials were conducted over many years with events advanced from the R0 analysis for each of the 35 constructs, and the performance of every plant for each construct in their first season of field trials was then analyzed as a set. Each construct was thus represented by many unique events. This allowed a larger number of constructs to be tested in first season field trials while only advancing beyond the first season trial the top performing constructs. Separate first season field trials were conducted with R2 plants (homozygous for each event) for (1) inbred efficacy for glufosinate+dicamba, quizalofop, and 2,4-D tolerance, (2) Roundup Hybridization System (RHS) efficacy, and (3) herbicide pressure testing for tolerance to higher application rates of herbicides quizalofop, 2,4-D, glufosinate, and dicamba.

[0230] An inbred efficacy screen of plants to evaluate herbicide tolerance for glufosinate+dicamba, quizalofop, and 2,4-D was carried out. Herbicide treatments consisted of: a tank-mix application of glufosinate at 0.8 lb ai / acre plus dicamba at 2.0 lb ae / acre; quizalofop at 0.16 lb ai / acre; or 2,4-D at 2.0 lb ai / acre. Plots were visually rated for crop injury 10-14 days after herbicide treatment on a scale of 0-100 with “0” being no crop injury and “100” being complete crop destruction. Plant height (PHT), ear height (EHT), days to 50% silk (S50D), days to 50% pollen (P50D), shell weight (SHW), test weight (TWT), moisture (MST), and grain yield (YLD) were also collected. All data were subjected to analysis of variance and means separation at p<0.05. Overall averages for multiple plants containing the same event were used, and inbred efficacy was summarized for glufosinate+dicamba, quizalofop, and 2,4-D as excellent (4), good (3), fair (2), or poor (1) or not applicable (NA). Data are provided in Table 3. Table 3. First season field trial inbred efficacy Construct Glufosinate / Dicamba Quizalofop 2,4-D101 US_ACTIVE\129309744\V-1Construct Glufosinate / Dicamba Quizalofop 2,4-D HT4-4 4 4 4dentify differences in glyphosate tolerance and glyphosate-induced tassel sterility of inbred material. A single herbicide treatment was used for screening and consisted of glyphosate at 1.5 lb ae / acre applied to V2 followed by102 US_ACTIVE\129309744\V-10.75 lb ae / acre to approximately V8 (875 growing degree days) followed by approximately V10 (1025 growing degree days). Plots were visually rated for % crop injury (CIPV2, CIPV8, CIPV10, and CIPVT) 10-14 days after herbicide application on a scale of 0 to 100 plus a final injury rating at VT (after tassel emergence). Plots were also visually rated for % silk emergence [(SES9A (S90) and SES9C (S90 plus 4 days) SES9E (S90 plus 8 days)] and % anther extrusion [(AES9A (S90), AES9C (S90 plus 4 days), and AES9E (S90 plus 8 days)] on a similar scale of 0 to 100 with “0” being no silk emergence or anther extrusion and “100” being complete silk emergence or anther extrusion. Other agronomic parameters were collected, as in the inbred efficacy screen. Overall averages for multiple plants containing the same event were used, and glyphosate tolerance, tassel sterility, and yield were each summarized as excellent (4), good (3), fair (2), or poor (1) or not applicable (NA). Data are provided in Table 4. Table 4. First season field trial RHS efficacy Construct Glyphosate Tolerance Tassel Sterility Yield103 US_ACTIVE\129309744\V-1Construct Glyphosate Tolerance Tassel Sterility Yield HT4-21 4 2 NA

[0232] An herbicide pressure test was conducted to evaluate construct level crop tolerance to higher application rates of herbicides quizalofop, 2,4-D, glufosinate, and dicamba as follows. Quizalofop treatments consisted of: 1) quizalofop at 0.32 lb ai / acre (4×) plus 0.25% v / v non-ionic surfactant (NIS) applied to VE-V2 followed by V4 followed by V8; 2) quizalofop at 0.64 lb ai / acre (8×) plus 0.25% v / v NIS applied to VE-V2 followed by V4 followed by V8; or 3) quizalofop at 1.28 lb ai / acre (16×) plus 0.25% v / v NIS applied to VE-V2 followed by V4 followed by V8. 2,4- D treatments consisted of 1) 2,4-D amine at 2 lb ai / acre plus 0.25% v / v non-ionic surfactant (NIS) applied to VE-V2 followed by V4 followed by V8; 2) 2,4-D amine at 4 lb ai / acre plus 0.25% v / v NIS applied to VE-V2 followed by V4 followed by V8; 3) 2,4-D amine at 8 lb ai / acre plus 0.25% v / v NIS applied to VE-V2 followed by V4 followed by V8; or 4) 2,4-D amine at 16 lb ai / acre plus 0.25% v / v NIS applied to VE-V2 followed by V4 followed by V8. Glufosinate treatments consisted of: 1) glufosinate 1.0 lb ai / acre applied to V2 followed by V4 followed by V8; 2) glufosinate 2.0 lb ai / acre applied to V2 followed by V4 followed by V8; 3) glufosinate 4.0 lb ai / acre applied to V2 followed by V4 followed by V8; or 4) glufosinate 8.0 lb ai / acre applied to V2 followed by V4 followed by V8. Dicamba treatments consisted of: 1) dicamba at 2.0 lb applied to V2 followed by V4 followed by V8; 2) dicamba at 4.0 lb applied to V2 followed by V4 followed by V8; or 3) dicamba at 8.0 lb applied to V2 followed by V4 followed by V8. 4) dicamba at 16 lb104 US_ACTIVE\129309744\V-1applied to V2 followed by V4 followed by V8. Plots were rated visually for crop injury and agronomic parameters were collected, as in the inbred efficacy screen. Overall averages for multiple plants containing the same event were used, and herbicide tolerance efficacy was summarized for each of the four herbicides as excellent (4), good (3), fair (2), or poor (1) or not applicable (NA). Data are provided in Table 5. Table 5. First season field trial herbicide pressure test Construct Glufosinate Dicamba Quizalofop 2,4-D HT4-1 4 4 3 3105 US_ACTIVE\129309744\V-1Construct Glufosinate Dicamba Quizalofop 2,4-D HT4-34 4 4 4 4f 35 constructs was compiled and analyzed for (1) the inbred efficacy test for glufosinate+dicamba, quizalofop, and 2,4-D tolerance, (2) the RHS efficacy test for glyphosate tolerance, tassel sterility, and yield, and (3) the herbicide pressure testing for tolerance to higher application rates of herbicides quizalofop, 2,4-D, glufosinate, and dicamba. Using this data, 3 constructs (HT4-14, HT4-32, and HT4-34) were selected for advancement from the 35 constructs tested. Events for these 3 constructs were then advanced to second season field trials. Example 3: Molecular Analysis

[0234] Molecular analysis was conducted concurrently with the field trials on events that were advanced. DNA amplification and sequencing were used to confirm the insert sequence, insert copy number, and absence of backbone in the insert. The insertion site in the maize genome for each event was mapped. Northern analysis was done to detect and measure mRNA transcripts of the pat, dmo, ft_t, and cp4-epsps genes. N-terminal protein sequencing of the PAT, DMO, FT_T, and CP4-EPSPS proteins purified from transgenic plants was done to confirm the recombinant protein sequence. Western blot analysis to detect the PAT, DMO, FT_T, and CP4-EPSPS proteins was done with transgenic plant samples. In depth Southern analysis was performed on genomic DNA from R1 plants to confirm copy number and the absence of backbone. Example 4: Advanced Field Trials

[0235] Advanced field trials (second season testing and beyond) were conducted over many years with events advanced from the first season field trials for constructs HT4-14, HT4-32, and HT4- 34. The performance of many individual plants for each event in each field trial was analyzed as a set. Each event was thus represented by many unique plants. This allowed the performance of106 US_ACTIVE\129309744\V-1each event to be analyzed under many conditions, in different locations and geographies, and for a variety of properties.

[0236] Field trials were conducted with inbred plants (homozygous for the event) and hybrid plants (hemizygous for the event) to assess (1) trait efficacy for commercial rates of glufosinate, dicamba, quizalofop, and 2,4-D tolerance, (2) agronomic performance, (3) Roundup Hybridization System (RHS) efficacy and glyphosate, and (4) herbicide pressure testing for tolerance to higher application rates of quizalofop, 2,4-D, glufosinate, and dicamba herbicides.

[0237] In the Field Season 1 trials, 30 events were tested for construct HT4-14, 41 events were tested for construct HT4-32, and 21 events were tested for construct HT4-34. Using the composite data from these trials, events were selected for advancement. In the Field Season 2 trials, 15 events were tested for the HT4-14 construct, 38 events for the HT4-32 construct, and 38 events for the HT4-34 construct (this number included some events not tested in Field Season 1 due to because of seed shortages during that season). Events for construct HT4-14 were characterized by molecular analysis as described in Example 3 and this data was also used to select events. The composite data from these trials and the in-depth molecular characterization of events for the HT4- 14 construct was used to select 3 events for advancement for the HT4-14 construct. The composite data from these trials was used to select 24 events for advancement for the HT4-32 construct and to decide not to advance any events for the HT4-34 construct. In the Field Season 3 trials, the 3 events for construct HT4-14 and 24 events for the HT4-34 construct were tested. The composite data from these trials was used to select 2 events for advancement for the HT4-14 construct and to decide not to advance any events for the HT4-32. The Field Season 4 trials were used to compare the final two events in a large number of locations, under a variety of conditions, and in hybrid and inbred germplasm in order to produce the data necessary to select the superior event. Table 6 provides the number of unique events tested for each construct in the field trials conducted during each season. Table 6. Advanced field trials summary Milestone HT4-14 HT4-32 HT4-34107 US_ACTIVE\129309744\V-1Milestone HT4-14 HT4-32 HT4-34 Field Season 3 3 24 0e season as the trait efficacy field trials. All field trials used a randomized complete block design and were conducted at multiple locations. Field trials were conducted at locations in North America and South America. For both efficacy and agronomic field trials, agronomic scoring was collected throughout the field trial season, and at the end of the season yield was determined (efficacy yield or agronomic yield). Efficacy field trials were conducted to assess crop injury 10 to 14 days following herbicide application. The target crop injury rating was a score of less than 10% for advancement of an event. For agronomic field trials, the plots were maintained weed free and none of the test herbicides were applied during the growing season. The hybrid agronomic field trials included controls of a comparable hybrid (hybrid control) produced using the same parental maize lines used to make the transgenic hybrid cross, but not containing a transgenic event. Inbred controls were a comparable inbred to the transgenic inbred lines.

[0239] To compare the field trial data, meta-analysis was performed using the aggregate of all plants in the multi-season, multi-location field trial data. As an example, Table 7 illustrates over multiple seasons the number of replications (reps) for which an observation was repeated for two selected HT4-14 events and the total number of individual plants tested in each trial for each event. Table 7. Field trial replications Milestone Event Total Reps Total Plants08 US_ACTIVE\129309744\V-1Milestone Event Total Reps Total Plants Field Season 4 MON87429 2465 172,550comparison of the hybrid injury ratings. As an example, Table 8 provides the injury rating over multiple seasons for two selected HT4-14 events scored at V8 (where V8 analysis encompasses the cumulative injury from V2, V4, V6, and V8 herbicide applications) with a statistical least significant difference at 95% confidence level (LSD at p<0.05). Plants containing maize event MON87429 and plants containing Event 2 both performed well in these trials. Table 8. Meta-analysis of injury rating from hybrid efficacy field trials Milestone Event V8 injury LSD (P<0.05) Field Season 1 MON87429 13 21

[0241] Meta-analysis of the multiple hybrid efficacy field trials was completed for comparison of yield as bushels / acre (Bu / ac). As an example, Table 9 provides yield from hybrids over multiple seasons for two selected HT4-14 events with a statistical least significant difference at 95% confidence level (LSD at p<0.05). Plants containing maize event MON87429 and plants containing Event 2 both performed well in these trials. Table 9. Meta-analysis of yield from hybrid efficacy field trials Milestone Event Yield (Bu / ac) LSD (p<0.05)109 US_ACTIVE\129309744\V-1

[0242] Pressure testing field trials with herbicide applied at higher application rates than commercial use were conducted with hybrid and inbred plants containing a single transgenic event. The herbicides glufosinate (over the range of 1.6 to 6.4 lb ai / acre), dicamba (over the range of 2.0 to 16 lb ai / acre), quizalofop (over the range of 0.32 to 1.28 lb ai / acre), 2,4-D (over the range of 2.0 to 8.0 lb ai / acre), and glyphosate (at 3.0 lb ae / acre) were applied in field trials for pressure testing of the efficacy of the herbicide tolerance traits. At the end of the season, the hybrid pressure testing field trials were harvested and yield (Bu / ac) was determined. As an example, Table 10 provides yield data from hybrids and inbreds in different trials for two selected HT4-14 events with a statistical least significant difference at 95% confidence level (LSD at p<0.05). Plants containing maize event MON87429 and plants containing Event 2 both performed well in hybrid and inbred yield trials for all of the herbicide treatments. The results suggested that further field testing could identify an inbred yield advantage for maize event MON87429 over Event 2. Table 10. Yield from hybrid / inbred pressure testing efficacy field trials Herbicide Plant Event Yield (Bu / ac) LSD (p<0.05) Glufosinate H brid MON87429 256 284

[0243] Hybrid agronomic field trials were conducted, agronomic measures were collected throughout the season, and agronomic yield was determined at the end of the season. Meta- analysis across the multi-season, multi-location hybrid agronomic field trials was used to compare the yield of the hybrid control and the hybrids. As an example, Table 11 provides yield data (Bu / ac) for two selected HT4-14 events with a statistical least significant difference at 95% confidence level (LSD at p<0.05). Plants containing maize event MON87429 and plants110 US_ACTIVE\129309744\V-1containing Event 2 both performed well in these trials, and no statistical difference in hybrid yield was found for plants containing either of these events when compared to the control plants. Table 11. Meta-analysis of yield from hybrid agronomic field trials Milestone Event Yield (Bu / ac) LSD (p<0.05) Field Season 1 Control - none 222 9

[0244] Inbred efficacy field trials were conducted for glyphosate tolerance and the Roundup Hybridization System (RHS) and yield was determined at the end of the season. Glyphosate was applied at a rate of 1.5 lb ae / acre for weed control followed by two sterility applications of glyphosate at 0.75 lb ae / acre at approximately V8 followed by 0.75 lb ae / acre at approximately V10. Meta-analysis across the multi-season, multi-location inbred efficacy field trials was used to compare the yield of plants. As an example, Table 12 provides yield data (Bu / ac) for two selected HT4-14 events with a statistical least significant difference at 95% confidence level (LSD at p<0.05). Field Season 2 and Field Season 3 trials both showed a statistically significant decrease in yield in plants containing Event 2 when compared to plants containing the Event MON87429. These data indicated the superior performance in inbred efficacy yield trials of plants containing maize event MON87429. Table 12. Meta-analysis of yield from glyphosate treated inbred efficacy field trials Milestone Event Yield (Bu / ac) LSD (p<0.05)111 US_ACTIVE\129309744\V-1Milestone Event Yield (Bu / ac) LSD (p<0.05) Field Season 3 EVENT 2 90.13 10.5he end of the season for untreated plants. The trials included controls of inbred lines comparable to the transgenic inbred lines. Meta-analysis across the multi-season, multi-location inbred agronomic field trials was conducted comparing yield for the paired control and the transgenic inbreds. As an example, Table 13 provides yield data (Bu / ac) for two selected HT4-14 events with a statistical least significant difference at 95% confidence level (LSD at p<0.05). No statistical difference in inbred agronomic yield was found between the control plants and plants containing maize event MON87429. In contrast, for Field Season 3 trials, there was a statistically significant decrease in yield in plants containing Event 2 when compared to control plants and plants containing maize event MON87429. These data indicated the superior performance in inbred agronomic yield trials of plants containing maize event MON87429. Table 13. Meta-analysis of yield from inbred agronomic field trials Milestone Event Total (Bu / ac) LSD (p<0.05) Fi ld S 1 C t l 1052 89

[0246] Hybrid efficacy trials were conducted at four locations in Argentina to evaluate plant tolerance to glufosinate, dicamba, quizalofop, haloxyfop, 2,4-D, and glyphosate. Plants containing MON87429 were crossed with plants containing both maize event MON88017 and maize event MON89034 to produce progeny containing all three events (MON87429×MON88017×MON89034). Herbicide treatments consisted of 1) a non-treated control; 2) glufosinate at 0.448 kg ai / ha applied at the V2 stage followed by the same application to the V6 stage; 3) glufosinate at 0.896 kg ai / ha applied at the V2 stage followed by the same112 US_ACTIVE\129309744\V-1application to the V6 stage; 4) dicamba at 0.56 lb ae / acre applied at the V2 stage followed by the same application to the V6 stage; 5) dicamba at 1.12 lb ae / acre applied at the V2 stage followed by the same application to the V6 stage; 6) quizalofop at 0.09 kg ae / ha applied at the V2 stage followed by the same application to the V6 stage; 7) quizalofop at 0.18 kg ae / ha applied at the V2 stage followed by the same application to the V6 stage; 8) haloxyfop at 0.1 kg ae / ha applied at the V2 stage followed by the same application to the V6 stage; 9) haloxyfop at 0.2 kg ae / ha applied at the V2 stage followed by the same application to the V6 stage; 10) 2,4-D at 1.12 lb ai / acre applied at the V2 stage followed by the same application to the V6 stage; 11) 2,4-D at 2.24 lb ae / acre applied at the V2 stage followed by the same application to the V6 stage; or 12) glyphosate at 2.24 lb ae / acre applied at the V2 stage followed by the same application to the V6 stage. Data collection consisted of crop injury 10 to 14 days after V2 and V6 herbicide applications and a final rating at VT, days to 50% pollen, days to 50% silk, plant height, ear height, shell weight, test weight, moisture, and grain yield. All data were subjected to analysis of variance and means separated at p<0.05.

[0247] Herbicide tolerance of plants containing MON87429×MON88017×MON89034 was excellent (<10% crop injury) over all rates of glyphosate, glufosinate, dicamba, quizalofop, haloxyfop, and 2,4-D tested. Herbicide treatment rates did not produce differences with respect to visual crop injury. Ear height was not significantly different for any of the treatments compared to the standard herbicide treatment 12 (glyphosate at 2.24 lb ae / acre). There was no significant reduction in plant height or test weight, increase in grain moisture, delay in maturity (measured as increase days to 50% pollen or silk), decrease in grain yield of the plants containing MON87429×MON88017×MON89034 within any treatment over that of non-treated plants. Hybrid plants produced through the cross of a plant containing MON87429 with a plant containing an event providing glyphosate tolerance in male tissues (such as commercially available maize events MON88017 or NK603) provide excellent vegetative tolerance to glyphosate, glufosinate, dicamba, quizalofop, haloxyfop, and 2,4-D when applied at commercial label rates.

[0248] Three years of field trials were used to test for control of plants comprising maize event MON87429 using clethodim. These trials assessed the use in volunteer control methods of a DIM herbicide with plants containing maize event MON87429. Plants were treated with clethodim at commercial label rates and complete control of plants comprising maize event MON87429 was observed.113 US_ACTIVE\129309744\V-1

[0249] The data accumulated from the molecular analysis and from the field trials with inbred and hybrid plants assessing (1) trait efficacy for commercial rates of glufosinate, dicamba, quizalofop, and 2,4-D tolerance, (2) agronomic performance, (3) Roundup Hybridization System (RHS) efficacy and glyphosate tolerance, and (4) herbicide pressure testing for tolerance to higher application rates of quizalofop, 2,4-D, glufosinate, and dicamba herbicides was analyzed for all the events tested for constructs HT4-14, HT4-32, and HT4-34. Analysis of the cumulative data demonstrated the overall superior performance of maize event MON87429 compared to the other events and resulted in selection of this event for commercial purposes. Example 5: Molecular Characterization of Maize Event MON87429

[0250] Maize event MON87429 was subjected to extensive molecular characterization upon selection as a commercial event. The transgenic insert of maize event MON87429 contains the elements and sequences described in Table 1.

[0251] DNA sequence analysis of maize event MON87429 was conducted. Southern blot analysis was conducted to confirm that plants containing maize event MON87429 contained a single, intact copy of the entire transgenic insert without any transformation vector backbone. Flanking DNA was sequenced on both the 5′ and 3′ ends of the insert, and the respective junctions were determined using sequence capture, enrichment, sequencing, inverse PCR, and genome walking techniques. The sequences of the flanking DNA for maize event MON87429 were mapped to the known maize genome physical assembly. The insertion site sequence information was used for bioinformatics analysis of the chromosomal location of the event. Insertion site integrity was determined by PCR across the wild-type allele using primers specific to the flanking regions of maize event MON87429. The wild-type insertion site was used to map the unique site of transgene integration for maize event MON87429 to the maize reference genome. To ensure that no alterations or mutations were introduced to any region of the transgene during transformation, the entire transgenic insert of maize event MON87429 was isolated from the plant and sequenced. Sequence information for the 5′ junctions, 3′ junctions, transgenic insert, and the entire event sequence are provided herein as SEQ ID NOs:1-10.

[0252] RNA analysis of plants comprising the construct of maize event MON87429 was conducted. Northern analysis was conducted on total RNA isolated from grain of plants containing maize event MON87429. This confirmed the transcript size and number for the pat, dmo, ft_t, and114 US_ACTIVE\129309744\V-1cp4-epsps mRNA products. RNA expression levels for CP4-EPSPS were also measured by real- time PCR using samples from plant tissues containing maize event MON87429. Rapid amplification of cDNA ends (RACE) was used to identify CP4-EPSPS cleavage products to confirm that cleavage of the CP4-EPSPS transcript occurs only in tassels of plants comprising maize event MON87429 and that this is triggered by maize endogenous male-specific small interference RNAs (siRNAs) in a sequence-specific manner. Low molecular weight Northern analysis was performed to demonstrate that there are no CP4-EPSPS siRNAs that could compromise glyphosate tolerance in non-tassel tissues.

[0253] Protein analysis of plants comprising the construct of maize event MON87429 was conducted. N-terminal protein sequencing of the expressed PAT, DMO, FT_T, and CP4-EPSPS proteins was performed using immunopurified protein extracts from grain to confirm the authentic N-terminal amino acid sequence. Western blot analysis was conducted on protein extracts from grain containing maize event MON87429 to confirm that a single expected-sized protein was being produced for PAT, DMO, FT_T, and CP4-EPSPS, respectively. ELISAs was used to determine protein levels in the leaf, seed, roots, and pollen of plants for the PAT, DMO, FT_T, and CP4- EPSPS proteins. Example 6: Detection of Maize Event MON87429

[0254] Detection of maize event MON87429 in a sample can be done using DNA, RNA, or protein detection techniques. Exemplary detection methods and materials are provided below. Detection may determine the presence or absence of maize event MON87429 in a sample. Detection may indicate the number of genomic copies of maize event MON87429 (that is, hemizygous, homozygous, or heterozygous) in a sample of genomic DNA.

[0255] An event specific endpoint Applied Biosystems™ TAQMAN® thermal amplification method (Thermo Fisher Scientific) was developed to identify maize event MON87429 in a sample. The DNA primers and probe used in the endpoint assay are primers SQ51062 (SEQ ID NO:11), SQ51053 (SEQ ID NO:12), and 6-FAM™ labeled probe PB50370 (SEQ ID NO:13). 6-FAM is a fluorescent dye product of Applied Biosystems (Foster City, Calif.) attached to the DNA probe. For TAQMAN MGB™ probes, the 5′ exonuclease activity of Taq DNA polymerase cleaves the probe from the 5′-end, between the fluorophore and quencher. When hybridized to the target DNA strand, quencher and fluorophore are separated enough to produce a fluorescent signal, thus115 US_ACTIVE\129309744\V-1releasing fluorescence. SQ51062 and SQ51053 when used with these reaction methods and PB50370 produce a DNA amplicon that is diagnostic for maize event MON87429. The controls for this analysis should include a positive control containing maize event MON87429, a negative control from non-transgenic maize, and a negative control that contains no template DNA. Additionally, a control for the PCR reaction should optimally include Internal Control Primers and an Internal Control Probe, specific to a single copy gene in the maize genome. These assays are optimized for use with the Applied Biosystems GeneAmp® PCR System 9700 (Thermo Fisher Scientific) run at maximum speed, but other equipment may be used.

[0256] An example of conditions useful with TAQMAN methods for detection of maize event MON87429 is as follows. Step 1: 18 megohm water adjusted for final volume of 5 μl. Step 2: 2.28 μl of 2× Universal Master Mix (dNTPs, enzyme, buffer) to a 1× final concentration. Step 3: 0.05 μl Event Primer-1 (SQ51062) and Event Primer-2 (SQ51053) (resuspended in 18 megohm water to a concentration of 100 uM for each primer) to 0.9 μM final concentration. Step 4: 0.01 μl Event 6-FAM MGB Probe PB50370 (resuspended in 18 megohm water to a concentration of 100 μM) to 0.2 μM final concentration. Step 5: 0.05 μl Internal Control Primer-1 and Internal Control Primer-2 Mix (resuspended in 18 megohm water to a concentration of 100 μM for each primer) to 0.9 μM final concentration. Step 6: 0.01 μl Internal Control VIC™ Probe (resuspended in 18 megohm water to a concentration of 100 μM) to 0.2 μM final concentration. Step 7: 2.5 μl Extracted DNA (template) for each sample with one each of the following comprising: (a) Leaf Samples to be analyzed; (b) Negative control (non-transgenic DNA); (c) Negative water control (no template); and (d) Positive control maize containing maize event MON87429 DNA. Step 8: Thermocycler Conditions as follows: one cycle at 95° C. for 20 seconds; forty cycles of 95° C. for 3 seconds then 60° C. for 20 seconds; and final cycle of 10° C.

[0257] A zygosity assay is developed to determine whether a plant comprising maize event MON87429 is heterozygous or homozygous for the event or the wild-type allele. An amplification reaction assay can be designed using the sequence information provided herein. For example, such a PCR assay would include design of at least three primers: primer-1, primer-2, and primer-3, where primer-1 is specific to maize genomic DNA on the 3′ flank of maize event MON87429; primer-2 is specific to maize event MON87429 transgenic insert; and primer-3 is specific to the wild-type allele. When used as a primer pair in an amplification reaction, primer-1 with primer-2 will produce a PCR amplicon specific for maize event MON87429. When used as a primer pair116 US_ACTIVE\129309744\V-1in an amplification reaction, primer-1 with primer-3 will produce a PCR amplicon specific for wild-type allele. In a PCR reaction performed on maize genomic DNA, the respective PCR amplicons generated from primer-1+primer-2 and that generated from primer-1+primer-3 will differ in sequence and size of the amplicon. When the three primers are included in a PCR reaction with DNA extracted from a plant homozygous for maize event MON87429, only the primer- 1+primer-2 amplicon (specific for the maize MON87429 insertion) will be generated. When the three primers are included in a PCR reaction with DNA extracted from a plant heterozygous for maize event MON87429, both the primer-1+primer-2 amplicon (specific for the maize MON87429 insertion) and the primer-1+primer-3 amplicon (specific for wild-type allele or absence of the maize MON87429 insertion) will be generated. When the three primers are mixed together in a PCR reaction with DNA extracted from a plant that is null for maize event MON87429 (that is wild-type), only the primer-1+primer-3 amplicon (specific for wild-type allele) will be generated. The amplicons produced using the PCR reaction may be identified or distinguished using any method known in the art.

[0258] Another zygosity assay for maize event MON87429 is a TAQMAN thermal amplification reaction. For this type of assay, in addition to primers as described above, the assay would include two fluorescently labeled probes. Probe-1 would be specific for maize event MON87429, and probe-2 would be specific for a maize plant that is null for maize event MON87429 (wild-type), and where the two probes contain different fluorescent labels, for example the 6-FAM-label or VIC™-label. When used in a TAQMAN reaction, primer-1+primer-2+probe-1 will produce a first fluorescent signal specific for maize event MON87429 and primer-1+primer-3+probe-2 will produce a second fluorescent signal specific for wild-type maize. When the three primers and two probes are included in a TAQMAN reaction with DNA extracted from a plant homozygous for maize event MON87429, only the first fluorescent signal (specific to primer-1+primer-2+probe- 1) will be generated. When the three primers are included in a TAQMAN reaction with DNA extracted from a plant heterozygous for maize event MON87429, both the first fluorescent signal (specific to primer-1+primer-2+probe-1) and the second fluorescent signal (specific to primer- 1+primer-3+probe-2) will be generated. When the three primers are mixed together in a TAQMAN reaction with DNA extracted from a plant which is null for maize event MON87429 (wild-type), only the second fluorescent signal (specific to primer-1+primer-3+probe-2) will be generated.117 US_ACTIVE\129309744\V-1

[0259] Another method to detect the presence of maize event MON87429 in a plant sample would be Southern analysis. One of the skill in art would understand how to design Southern hybridization probe(s) specific for maize event MON87429 and a second southern hybridization probe specific for a maize plant which is null for maize event MON87429 (wild-type). With Southern analysis, a signal detected only from the first Southern hybridization probe will be indicative of a plant homozygous for maize event MON87429; a signal detected from both the first Southern hybridization probe and the second Southern hybridization probe will be indicative of a plant heterozygous for maize event MON87429; and a signal detected only from the second Southern hybridization probe will be indicative that the DNA was extracted from a plant that is null for maize event MON87429 (wild-type).

[0260] Another example of a detection kit comprises at least one antibody specific for at least one protein encoded by maize event MON87429. For example, such a kit may utilize a lateral flow strip comprising reagents activated when the tip of the strip is contacted with an aqueous solution. Exemplary proteins sufficient for use in antibody production are ones encoded by the sequence provided as SEQ ID NO:10, or any fragment thereof.

[0261] A protein detection method is developed to determine whether a sample is from a plant, seed, cell, or plant part comprising maize event MON87429. At least one antibody specific for at least one protein encoded by maize event MON87429 is used to detect a protein encoded by maize event MON87429 in a sample. A detection kit comprising one or more antibodies specific for one or more proteins encoded by maize event MON87429 may utilize a lateral flow strip containing reagents activated when the tip of the strip is contacted with an aqueous solution. Samples of maize tissue may be ground up and protein extracted for analysis using water or an aqueous buffer ...

Claims

What is claimed is:

1. A recombinant DNA molecule comprising: a) a first nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO: 9; and b) a second nucleotide sequence that i. comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16, or ii. is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16, or iii. is selected from the group consisting of SEQ ID NOs:18-117; and wherein the recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:

10.

2. The recombinant DNA molecule of claim 1, further comprising: c) a third nucleotide sequence that198 US_ACTIVE\129309744\V-1i. comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or ii. is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or iii. is selected from the group consisting of SEQ ID NOs:118-217.

3. The recombinant DNA molecule of claim 2 wherein the recombinant DNA molecule has a deletion of one or more consecutive nucleotides between the second nucleotide sequence and the third nucleotide sequence relative to SEQ ID NO:10, 15, or 17.

4. The recombinant DNA molecule of any one of claims 1-3, wherein the recombinant DNA molecule has a deletion of one or more consecutive nucleotides between the first nucleotide sequence and the second nucleotide sequence relative to SEQ ID NO:10, 14, or 16.

5. A recombinant DNA molecule comprising: a) a first nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9; and b) a second nucleotide sequence that199 US_ACTIVE\129309744\V-1i. comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or ii. is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8% or at least 99.9% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17, or iii. is selected from the group consisting of SEQ ID NOs:118-217; and wherein the recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:

10.

6. The recombinant DNA molecule of claim 5, wherein the recombinant DNA molecule has a deletion of one or more consecutive nucleotides between the first nucleotide sequence and the second nucleotide sequence relative to SEQ ID NO:10, 15, or 17.

7. A recombinant DNA molecule comprising a first nucleotide sequence having at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9%, or 100% identical to the full length of SEQ ID NO: 9; wherein the recombinant DNA200 US_ACTIVE\129309744\V-1molecule further comprises at the 5’ or 3’ end of said first nucleotide sequence a second nucleotide sequence comprising: a) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:14 or 16; and / or b) at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:15 or 17; and wherein the recombinant DNA molecule comprises at least one substitution, insertion, deletion, inversion, or a combination of any thereof, as compared to SEQ ID NO:

10.

8. The recombinant DNA molecule of claim 7, wherein: a) the recombinant DNA molecule comprises at the 5’ end of said first nucleotide sequence one or more nucleotide sequences selected from SEQ ID NOs:18–117; and / or b) the recombinant DNA molecule comprises at the 3’ end of said first nucleotide sequence one or more nucleotide sequences selected from SEQ ID NOs:118–217.

9. The recombinant DNA molecule of any one of claims 1-8, wherein the recombinant DNA molecule is comprised in a corn plant, corn plant part, corn plant cell, corn plant seed, corn progeny plant, or commodity or fuel product made from corn and corn plant parts.

10. The recombinant DNA molecule of any one of claims 1-8, wherein the recombinant DNA molecule comprises an amplicon diagnostic for the presence of DNA comprising a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:9.201 US_ACTIVE\129309744\V-111. The recombinant DNA molecule of any one of claims 1-8, wherein the recombinant DNA molecule is derived from a corn plant, corn plant part, corn seed, processed corn seed, corn plant cell or tissue, animal feed comprising corn, corn oil, corn meal, corn flour, corn flakes, corn bran, food made comprising corn, corn biomass, or fuel products made from corn and corn plant parts.

12. A recombinant DNA molecule comprising a polynucleotide segment of sufficient length to function as a DNA probe that hybridizes specifically under stringent hybridization conditions with a polynucleotide of a modified corn event MON87429 DNA in a sample, wherein the polynucleotide comprises a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, SEQ ID NO:17 and complete complements of any thereof, and wherein detecting hybridization of the polynucleotide under the stringent hybridization conditions is diagnostic for the presence of the modified corn event MON87429.

13. A pair of DNA molecules comprising a first DNA molecule and a second DNA molecule that function as DNA primers when used together in an amplification reaction with DNA comprising a corn modified event MON87429 to produce an amplicon diagnostic for the modified corn event MON87429 in a sample, wherein the first DNA molecule and the second DNA molecule are different from each other and a) wherein the first DNA molecule is: i. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ202 US_ACTIVE\129309744\V-1ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof, or ii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement of any thereof; and b) wherein the second DNA molecule is: i. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof, or ii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at203 US_ACTIVE\129309744\V-1least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement of any thereof, or iii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, or at least 99.9% identical or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement of any thereof.

14. The pair of DNA molecules of claim 13, wherein the amplicon comprises a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:

10.

15. A method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample, said method comprising: a) contacting said sample with the recombinant DNA molecule of claim 12; b) subjecting said sample and said recombinant DNA molecule to stringent hybridization conditions; and c) detecting hybridization of said recombinant DNA molecule to said DNA segment in said sample, wherein said detection is diagnostic for the presence of said modified corn event MON87429 DNA in said sample.

16. A method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample, said method comprising: a) contacting said sample with the pair of DNA molecules of claim 13; b) performing an amplification reaction sufficient to produce a DNA amplicon; and c) detecting the presence of said DNA amplicon in said reaction,204 US_ACTIVE\129309744\V-1wherein the presence of said DNA amplicon is diagnostic for the presence of said modified corn event MON87429 DNA in said sample.

17. A method of detecting the presence of a DNA segment diagnostic for a modified corn event MON87429 DNA in a sample, said method comprising performing a sequencing reaction to produce a target nucleotide sequence, wherein said target nucleotide sequence comprises at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement of any thereof.

18. A modified corn plant, corn plant part, corn seed, or corn cell comprising a modified corn event MON87429.

19. The modified corn plant, corn plant part, corn seed, or corn cell of claim 18, comprising: a) the recombinant DNA molecule of any one of claims 1-8; or b) a DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, or a complement thereof; or c) a DNA molecule comprising a nucleotide sequence that is at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, at least 50, at least 60, at least 70, at least 80, at least 90, at least 100, at least 150, at least 200, at least 250, at least 300, at least 400, at least 500, at least 1,000, at least 1,500, or at least 2,000 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, and SEQ ID NO:10, SEQ ID205 US_ACTIVE\129309744\V-1NO:14, SEQ ID NO:15, SEQ ID NO:16, or SEQ ID NO:17, or a complement of any thereof.

20. The modified corn plant, corn plant part, corn seed, or corn cell of claim 18 or 19, wherein the corn plant, corn plant part, corn seed, or corn cell is further defined as a progeny plant of any generation of a corn plant comprising a modified corn event MON87429, or a corn plant part, corn seed, or corn cell derived therefrom.

21. The modified corn plant, corn plant part, corn seed, or corn cell of any of claims 18-20, wherein the corn plant, corn plant part, corn seed, or corn cell exhibits tolerance to one or more herbicides selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof.

22. A DNA detection kit comprising: a) the DNA probe of claim 12; or b) the pair of DNA molecules of claim 13 or 14.

23. A method of producing a progeny corn plant comprising a modified corn event MON87429 comprising: a) sexually crossing a first corn plant that comprises the modified corn event MON87429 with itself or a second corn plant; b) collecting one or more seeds produced from said cross; c) growing said seed to produce one or more progeny plants; and d) selecting at least a first progeny plant or seed comprising the modified corn event MON87429.

24. The method of claim 23, wherein selecting at least a first progeny plant or seed comprising the modified corn event MON87429 comprises treating the progeny plant or seed with an effective amount of at least one herbicide selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5-206 US_ACTIVE\129309744\V-1enolpyruvylshikimate-3-phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof.

25. A hybrid modified corn plant or seed comprising a modified corn event MON87429 produced by the method of claim 23 or 24.

26. The method of claims 23 or 24, further comprising: e) collecting seed from said at least first progeny plant comprising the modified corn event MON87429.

27. A nonliving corn plant material comprising a detectable amount of the recombinant DNA molecule of any one of claims 1-9.

28. A microorganism comprising the recombinant DNA molecule of any one of claims 1-8.

29. A plant cell comprising the recombinant DNA molecule of any one of claims 1-9.

30. A commodity product comprising the recombinant DNA molecule of any one of claims 1-9.

31. The commodity product of claim 30, wherein said commodity product is produced from a modified corn plant, corn plant part, corn seed, or corn tissue or cell comprising a modified corn event MON87429.

32. The commodity product of claim 30 or 31, wherein the commodity product is selected from the group consisting of whole or processed corn seed; food for human consumption or animal feed such as corn oil, corn meal, corn flour, corn grits, corn flakes, corn bran, or corn starch; sweetener such as high fructose corn syrup (HFCS), glucose or dextrose; beverage alcohol; brewer grits for beer production; fiber; corn biomass; fuel products produced using corn and corn plant parts; corn pollen; corn plastic; dried distillers grains (DDGs); and bio-degradable packing material.

33. A method of producing a commodity product, said method comprising: a) obtaining a modified corn plant, corn plant part, or corn seed comprising a modified corn event MON87429; and b) producing a commodity product from the modified corn plant, corn plant part, or corn seed.

34. A corn plant, corn plant part, or corn seed comprising a DNA molecule or segment functional as a template when tested in a DNA amplification method to produce an amplicon diagnostic for the presence of a modified corn event MON87429 DNA.207 US_ACTIVE\129309744\V-135. A method of determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 comprising: a) contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a first primer pair that can produce a first amplicon of all or part of the modified corn event MON87429 and a second primer pair that can produce a second amplicon of a standard genomic sequence known to be single copy and homozygous in the corn plant, corn plant part, or corn seed; b) contacting the sample with a first probe that specifically hybridizes to the first amplicon and / or all or part of the modified corn event MON87429, and a second probe that specifically hybridizes to the standard genomic sequence; c) performing a DNA amplification reaction using real-time PCR with the sample and determining the cycle thresholds (Ct values) of the first amplicon and the second amplicon; d) calculating the difference (ΔCt) between the Ct values of the second amplicon and the first amplicon; and e) determining the zygosity of the modified corn event MON87429, wherein a ΔCt of about zero (0) indicates homozygosity of the modified corn event MON87429 and a ΔCt of about one (1) indicates heterozygosity of the modified corn event MON87429.

36. A method of determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 comprising: a) contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a primer pair capable of producing a first amplicon diagnostic for the modified corn event MON87429 and a second amplicon diagnostic for native corn genomic DNA not comprising the modified corn event MON87429; b) performing a nucleic acid amplification reaction with the sample and the set of primer pairs; and c) detecting the first amplicon and the second amplicon, wherein the presence of only the first amplicon is diagnostic of a corn plant, corn plant part, or corn seed homozygous for the modified corn event MON87429, the presence of only the second amplicon is diagnostic of a corn plant, corn plant part, or corn seed208 US_ACTIVE\129309744\V-1homozygous for native corn genomic DNA not comprising the modified corn event MON87429, and the presence of both the first amplicon and the second amplicon is diagnostic of a corn plant, corn plant part, or corn seed heterozygous for the modified corn event MON87429.

37. A method for determining the zygosity of a corn plant, corn plant part, or corn seed comprising a modified corn event MON87429 comprising: a) contacting a sample comprising DNA from the corn plant, corn plant part, or corn seed with a probe set which contains at least a first probe that specifically hybridizes to the modified corn event MON87429 and at least a second probe that specifically hybridizes to corn genomic DNA that was disrupted by insertion of the heterologous DNA of corn event MON87429 and is disrupted by the modified corn event MON87429 DNA, wherein the second probe does not hybridize to the modified corn event MON87429 DNA; and b) hybridizing the probe set with the sample under stringent hybridization conditions, wherein detecting hybridization of only the first probe under the hybridization conditions is diagnostic for a corn plant, corn plant part, or corn seed homozygous for the modified corn event MON87429, and wherein detecting hybridization of both the first probe and the second probe under the hybridization conditions is diagnostic for a corn plant, corn plant part, or corn seed heterozygous for the modified corn event MON87429.

38. A population of transgenic corn plants, wherein each transgenic corn plant comprises a modified corn event MON87429.

39. The population of claim 38, wherein each transgenic corn plant of the population exhibits tolerance to at least one herbicide selected from the group consisting of inhibitors of acetyl CoA carboxylase (ACCase) in the aryloxyphenoxy propionate (FOP) group such as quizalofop and haloxyfop; synthetic auxins such as dicamba and 2,4-D; inhibitors of glutamine synthetase such as glufosinate; inhibitors of 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) such as glyphosate; and combinations of any thereof.

40. A method of modifying a corn plant comprising event MON87429, the method comprising: a) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an209 US_ACTIVE\129309744\V-1explant of a corn plant comprising corn event MON87429, or a plant part thereof, to produce a modified corn event MON87429 via a genome editing technique; and b) developing or regenerating a modified corn plant comprising the modified corn event MON87429.

41. The method of claim 40, wherein the site-specific nuclease is a zinc-finger nuclease (ZFN), a meganuclease, an RNA-guided endonuclease, a TALE-endonuclease (TALEN), a recombinase, or a transposase.

42. The method of claim 40 or 41, wherein the site-specific nuclease is a CRISPR / Cas nuclease.

43. The method of claim 42, wherein the introducing step (a) comprises introducing the recombinant DNA construct into the at least one cell of the explant, and wherein the recombinant DNA construct further comprises an expression cassette encoding a first guide RNA (gRNA).

44. The method of claim 43, wherein the recombinant DNA construct further comprises an expression cassette encoding a second guide RNA (gRNA).

45. The method of claim 42, wherein the introducing step (a) further comprises introducing a first guide RNA (gRNA) or a second recombinant DNA construct comprising an expression cassette encoding a first guide RNA (gRNA) into the at least one cell of the explant.

46. The method of claim 42, wherein the introducing step (a) comprises introducing at least two guide RNAs (gRNAs) comprising a first gRNA and a second gRNA into the at least one cell of the explant.

47. The method of claim 45, wherein the second recombinant DNA construct further comprises an expression cassette encoding a second guide RNA (gRNA).

48. The method of claim 42, wherein the introducing step (a) comprises introducing a first gRNA and second recombinant DNA construct comprising an expression cassette encoding a second guide RNA (gRNA) into the at least one cell of the explant.

49. The method of any one of claims 40-48, wherein the site-specific nuclease has a first target site in the genome of the corn plant at or near corn event MON87429.

50. The method of claim 49, wherein the site-specific nuclease has a second target site in the genome of the corn plant at or near corn event MON87429.210 US_ACTIVE\129309744\V-151. The method of any one of claims 40-50, wherein the introducing step (a) further comprises introducing a second site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a second site specific nuclease into at least one cell of the explant, and wherein the second site-specific nuclease has a second target site in the genome of the corn plant at or near corn event MON87429.

52. The method of any one of claims 43-48, wherein the first gRNA has a first target site in a flanking DNA sequence, 5´ flank, 3´ flank, junction sequence, or T-DNA insertion sequence of corn event MON87429, or a complement thereof.

53. The method of any one of claims 43-48 and 52, wherein the first gRNA has a first target site comprising a target sequence that is: i. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or ii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or iii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least211 US_ACTIVE\129309744\V-199.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.

54. The method of claim 43-48, 52, and 53, wherein the first gRNA has a second target site comprising a target sequence that is: i. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or ii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or iii. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to212 US_ACTIVE\129309744\V-1at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.

55. The method of any one of claims 44-48 and 52-54, i. wherein the first gRNA has a first target site comprising a target sequence that is: a. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or b. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or c. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%,213 US_ACTIVE\129309744\V-1at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof; and ii. wherein the second gRNA has a second target site comprising a target sequence that is: a. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:14 or 16, or a complement thereof; or b. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at214 US_ACTIVE\129309744\V-1least 50 consecutive nucleotides of SEQ ID NO:15 or 17, or a complement thereof; or c. at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, at least 99.1%, at least 99.2%, at least 99.3%, at least 99.4%, at least 99.5%, at least 99.6%, at least 99.7%, at least 99.8%, at least 99.9% or 100% identical to at least 10, at least 11, at least 12, at least 13, at least 14, at least 15, at least 16, at least 17, at least 18, at least 19, at least 20, at least 21, at least 22, at least 23, at least 24, at least 25, at least 30, at least 35, at least 40, at least 45, or at least 50 consecutive nucleotides of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, or SEQ ID NO:9, or a complement thereof.

56. The method of any one of claims 50, 51, 54, and 55, wherein the modified corn event MON87429 comprises a deletion or excision of intervening DNA between the first target site and the second target site, relative to the corn event MON87429.

57. The method of any one of claims 40-56, further comprising: c) selecting the modified corn plant comprising the modified corn event MON87429; and d) sexually crossing the modified corn plant with itself or a second corn plant to produce one or more modified progeny corn plants.

58. A method of introducing a target site into a corn plant, the method comprising: a) introducing a cognate target site into the corn event MON87429 locus of at least one cell of a corn plant or corn plant part comprising the corn event MON87429 or an explant thereof via a targeted genome editing technique, wherein the cognate target site is identical or similar to an originator target site for a site- specific nuclease present in the corn event MON87429 locus; and b) developing or regenerating a modified corn plant comprising a modified corn event MON87429 comprising the cognate target site.

59. The method of claim 58, further comprising:215 US_ACTIVE\129309744\V-1c) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a corn plant comprising the modified corn event MON87429 or a plant part thereof, to produce a further modified corn event MON87429 via a targeted genome editing technique, wherein the target site of the site-specific nuclease includes the cognate target site and the originator target site; and d) developing or regenerating a second modified corn plant comprising the further modified corn event MON87429.

60. A method of introducing a target site into a corn plant, the method comprising: a) introducing a cognate target site into the corn event MON87429 locus of at least one cell of a corn plant or corn plant part comprising the corn event MON87429 or an explant thereof via a targeted genome editing technique to produce a modified corn event MON87429 comprising the cognate target site, wherein the cognate target site is identical or similar to an originator target site for a site- specific nuclease present in the corn event MON87429 locus, and b) introducing a site-specific nuclease or a recombinant DNA construct comprising an expression cassette encoding a site-specific nuclease into at least one cell of an explant of a modified corn plant comprising the modified corn event MON87429, or a plant part thereof, to produce a further modified corn event MON87429 via a targeted genome editing technique, wherein the target site of the site-specific nuclease includes the cognate target site and the originator target site; and c) developing or regenerating a second modified corn plant comprising the further modified corn event MON87429.

61. The method of claim 59 or 60, wherein the further modified corn event MON87429 of the second modified corn plant comprises a deletion or excision of intervening DNA between the originator target site and the cognate target site, relative to the corn event MON87429 or modified corn event MON87429.

62. The method of any one of claims 59-61, further comprising: selecting the second modified corn plant or a progeny plant of the second modified corn plant comprising the further modified corn event MON87429, and sexually crossing the second modified corn216 US_ACTIVE\129309744\V-1plant or the progeny plant with itself or another corn plant to produce one or more modified progeny corn plants comprising the further modified corn event MON87429.217 US_ACTIVE\129309744\V-1

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