Bioprocessing base, bioprocessing station and bioprocessing system

Abstract

Shown and described is a bioprocessing base (1) for performing a bioprocess, comprising: a base body (2) comprising: base alignment means (3), one or more interface elements (4) adapted to support and preferably accommodate a bioprocess component (18), and pumping means (8), preferably a peristaltic pump head; an adapter module (9) comprising: one or more fluidic valve actuators (10); and optionally at least one electrical connector (11). In order to perform different and / or multiple operations for a bioprocess, preferably cell and / or gene therapy, while providing an improved handling, the described bioprocessing base (1) is proposed. In addition a bioprocessing station (17), a bioprocessing system (27), a use of a bioprocess component (18) in combination with a bioprocessing base (1) and a method of processing a biological sample in a bioprocessing system (27) are also described and shown intended for performing different and / or multiple operations for a bioprocess, preferably cell and / or gene therapy, while providing an improved handling.

Description

[0001] Bioprocessing base, bioprocessing station and bioprocessing system

[0002] The present invention relates to a bioprocessing base for performing a bioprocess, comprising a base body comprising base alignment means, one or more interface elements adapted to support and preferably accommodate a bioprocess component, and pumping means, preferably a peristaltic pump head; an adapter module comprising: one or more fluidic valve actuators; and optionally at least one electrical connector.

[0003] The present invention further relates to bioprocessing station comprising a bioprocessing base according to any one of claims 1 to 8 and at least one bioprocess component.

[0004] The present invention further relates to a bioprocessing system comprising a housing and at least two bioprocessing bases according to any one of claims 1 to 8, preferably at least two bioprocessing stations according to any one of claims 9 to 12.

[0005] The present invention further relates to the use of a bioprocess component in combination with a bioprocessing base according to any one of claims 1 to 8 for performing a bioprocess, preferably of a bioprocessing station according to any one of claims 9 to 12 for performing a bioprocess.

[0006] The present invention further relates to a method of processing a biological sample in a bioprocessing system according to any one of claims 13 to 18, the method comprising: arranging at least two bioprocessing stations in adjacent processing positions, generating a fluid-tight connection between the bioprocessing stations, preferably by welding, wherein preferably the reversible fluid-tight connection is established through a consumable element which may comprise a tube set and / or a bioprocess container, performing a bioprocess, and optionally severing the fluid-tight connection, preferably by sealing and cutting.

[0007] In recent years, the field of cell and gene therapy has garnered increasing attention. As promising therapies multiply, the focus intensifies on scaling up production systems. Currently multiple independent devices, which devices respectively perform only one unique operation for cell and / or gene therapy production, are usually used. Therefore, many different devices are needed to perform the different operations for cell and / or gene therapy production. Some biotech firms are actively pursuing fully automated and integrated systems to simultaneously produce several batches of immune or naive cell cultures for cell and gene therapy. However, these systems usually do not support an easy change of system components, for example to adapt the system for different production conditions or to upgrade the system, and often do not allow for an individual, preferably also manual, placement and / or replacement of consumable elements. The handling of these systems outside the rigid processes is thus often cumbersome.

[0008] Against this background, it is an object of the present invention to provide a bioprocessing base, a bioprocessing station, a bioprocessing system, a use of a bioprocess component with a bioprocessing base and a method of processing a biological sample in a bioprocessing system which can perform different and / or multiple operations for a bioprocess, preferably cell and / or gene therapy, while providing an improved handling.

[0009] The object named above is solved in accordance with the present invention by the bioprocessing base according to claim 1.

[0010] The object named above is further solved in accordance with the present invention by the bioprocessing station according to claim 9. The object named above is further solved in accordance with the present invention by the bioprocessing system according to claim 13.

[0011] The object named above is further solved in accordance with the present invention by the use of a bioprocess component in combination with a bioprocessing base according to claim 19.

[0012] The object named above is further solved in accordance with the present invention by the method of processing a biological sample in a bioprocessing system according to claim 22.

[0013] Various embodiments of the bioprocessing base, bioprocessing station, bioprocessing system, use of a bioprocess component with a bioprocessing base and method of processing a biological sample in a bioprocessing system are described in the following. The individual embodiments are in each case individually applicable to the bioprocessing base, bioprocessing station, bioprocessing system, use of a bioprocess component with a bioprocessing base and / or method of processing a biological sample in a bioprocessing system. The individual embodiments may furthermore be combined with each other at will.

[0014] The bioprocessing base for performing a bioprocess comprises a base body, wherein the base body comprises base alignment means, one or more interface elements adapted to support and preferably accommodate a bioprocess component, and pumping means, preferably a peristaltic pump head. The bioprocessing base can comprise a frame, wherein the base alignment means, the one or more interface elements and / or the pumping means can be connected to the frame and / or be part of the frame. The bioprocessing base is a mobile bioprocessing base. This can be used to insert the bioprocessing base into and / or remove the bioprocessing base out of a housing of a bioprocessing system, for example to provide and / or change one or more bioprocess components on the bioprocessing base. The base alignment means simplify an easy insertion and / or removal of the bioprocessing base into and / or from a housing of a bioprocessing station. This further simplifies equipping the bioprocessing station with the bioprocessing bases needed to perform a certain bioprocess and / or to easily remove and / or insert the bioprocessing base into the housing of the bioprocessing system to change one or more consumable elements provided in one or more bioprocess components on the bioprocessing base. The base alignment means are in particular used to align the position and / or orientation of the base body in the housing of the bioprocessing system. The base alignment means can for example be provided as one or more, preferably at least in sections oppositely arranged, protruding edges. This provides for a sufficient alignment with a constructively simple design. The protruding edges can be part of the frame and / or protrude from the frame. This further simplifies the construction of the bioprocessing base. A consumable element in the present disclosure is preferably a single-use consumable element. This helps minimizing the set-up time as the time for cumbersome cleaning can be reduced.

[0015] The one or more interface elements simplify performing different operations on the bioprocessing base and thus also in the bioprocessing system, as the one or more interface elements can be configured for interacting with the bioprocess component supported, preferably accommodated, by the bioprocessing base in order to perform one or more, preferably different, operations, preferably unit operations, of a bioprocess, preferably in the bioprocess component. A unit operation herein preferably refers to a unit operation used for cell therapy manufacture, e.g. for manufacture of therapeutic immune cells, such as CAR T cells or NK cells. Unit operations in context of cell therapy manufacturing process may include cell enrichment, cell activation, cell selection and / or genetic modification of cells. As used herein a unit operation is different from a step of expanding cells (even though cells may also proliferate and thus expand throughout a unit operation). According to a preferred embodiment, the one or more unit operations are selected from: activation of the cells, preferably wherein the liquid composition comprises an activation agent, genetic modification of the cells, in particular transduction and / or transfection, preferably wherein the liquid composition comprises a genetic modification agent, in particular a transduction agent and / or a transfection agent. Both of activation of the cells and genetic modification of the cells is usually required in order to manufacture a cell therapy product, in particular cells which have been genetically modified, e.g. to target a specific cell type such as a cancer cell. This particularly refers to manufacture of CAR T cells or other immune cell types which are usually genetically modified for targeting a cell type of interest. It can also be provided that the one or more interface elements are adapted to support and preferably accommodate one or more bioprocess components, preferably at least two bioprocess components, and / or that each interface element of the one or more interface elements supports at least one bioprocess component. Moreover, the one or more interface elements can comprise at least two interface elements. These different designs simplify it that each bioprocess component can be individually supported by the one or more interface elements, thus simplifying an individual setup of the different bioprocess components on the bioprocessing base. Furthermore the one or more interface elements are preferably arranged in such a way that a bioprocess component can be moved relative to and / or above the one or more interface elements, thus simplifying swapping of bioprocess components.

[0016] The pumping means, preferably a peristaltic pump head, also helps in performing different operations, preferably unit operations, of a bioprocess by the bioprocessing base itself. The pumping means is configured for interacting with a tube, preferably of a tube set of the bioprocess component, in order to convey fluid, preferably to and / or away from the bioprocess component.

[0017] In addition, the bioprocessing base comprises an adapter module, wherein the adapter module comprises one or more fluidic valve actuators. The adapter module simplifies connecting the bioprocessing base to a bioprocessing system and / or another bioprocessing base, as with the adapter module a designated element is provided for the connection. The fluidic valve actuators are preferably configured for interacting with a tube and / or multifluidic cassette, preferably of a tube set of a bioprocess component provided on the bioprocessing base, in order to convey fluid, preferably to and / or away from the bioprocess component. The adapter module can be arranged at an end of the bioprocessing base and / or in a row with the one or more interface elements. In this way the adapter module does not obstruct the placement of one or more bioprocess components on the bioprocessing base.

[0018] In addition, it can be optionally provided that the bioprocessing base comprises at least one electrical connector. The electrical connector is used for receiving power for the bioprocessing base in order to power the different components of the bioprocessing base, for example the one or more interface elements, the pumping means and / or the fluidic valve actuators. The power received via the electrical connector can also be used for one or more bioprocess components provided on the bioprocessing base. The electrical connector can be arranged on the base body and / or the adapter module. In addition, the bioprocessing base, preferably the base body and / or adapter module, can also comprise a data connector for receiving data for and / or transmitting data from the bioprocessing base. This simplifies controlling and / or monitoring the bioprocessing base.

[0019] In an embodiment of the bioprocessing base it is provided that the adapter module further comprises locking means for reversibly mounting the bioprocess component at a fixed position. In this way the bioprocess component is safely secured to the bioprocessing base. This also simplifies a safe handling of the bioprocessing base when the bioprocess component is arranged on the bioprocessing base. The locking means can be configured to be adjustable between a, preferably normally, closed position for mounting the bioprocess component at the fixed position and an open position for releasing the bioprocess component. By providing a normally closed position, the safe fixation of the bioprocess component is ensured even if the bioprocess component is cut-off from power. The locking means can preferably be a pair of cam locks. These provide a secure and constructively simple fixation. Moreover, the locking elements can be arranged facing the one or more interface elements, thus allowing for a fixation of the bioprocess component directly at the adapter module when the bioprocess component is supported by the one or more interface elements. This also helps in simplifying a secure fluidic and / or electrical connection between the bioprocess component and the bioprocessing base.

[0020] The term “reversibly” throughout this disclosure is preferably to be understood as “detachably”.

[0021] In an embodiment of the bioprocessing base it is provided that at least one, preferably each, of the interface elements comprises means for determining the weight of the bioprocess component, means for controlling and adjusting the temperature of the bioprocess component, and / or means for agitating the bioprocess component. In this way the bioprocessing base can perform, preferably different, operations, preferably unit operations, of a bioprocess on a bioprocess component supported by the one or more interface elements. The means for determining the weight of the bioprocess component can preferably comprise a load cell, thus providing a compact and reliable means for determining the weight. The means for controlling and adjusting the temperature of the bioprocess component can comprise a heating element and / or a cooling element. The means for agitating the bioprocess component can comprise a magnetic device for employing a rotating magnetic field. In this way the means for agitating the bioprocess component can act, preferably together with a stir bar provided in the bioprocess component, as a magnetic stirrer. In this case the magnetic device is configured to rotate the stir bar. As a result, only minimal set-up is required to agitate the bioprocess component, thus simplifying the handling.

[0022] In an embodiment of the bioprocessing base it is provided that at least one, preferably each, of the interface elements comprises interface alignment means for positioning and optionally retaining the bioprocess component, such as a magnetic retention system. In this way the bioprocess component can be optimally positioned. By providing each interface element of the one or more interface elements with an alignment means, also multiple bioprocess components can be aligned and preferably retained individually, thus simplifying an individual setup of the bioprocess components on the bioprocessing base. This also improves the handling of the bioprocessing base. Using a magnetic retention system further improves the handling as a formfitting connection, which is often associated with a more complex movement sequence during the connection process, to retain the bioprocess component does not need to be established. The interface alignment means, preferably the magnetic retention system, can comprise one or more electromagnets and / or one or more permanent magnets. This provides for a secure retention. Alternatively or in addition, the interface alignment means, preferably the magnetic retention system, can be adjustable between a retention state for positioning and preferably retaining the bioprocess component, preferably via magnetic force, and a release state for releasing the bioprocess component. In this way dedicated states for retaining and releasing the bioprocess component are provided, thus simplifying retention and realse.

[0023] In an embodiment of the bioprocessing base it is provided that the bioprocessing base comprises an alignment system for aligning the bioprocess component with the bioprocessing base and, preferably, reversibly fixing it thereto. This further improves the positioning and preferably fixation of the bioprocess component. The alignment system is preferably configured for form-fitting alignment and / or fixation of the bioprocess component with the bioprocessing base. Preferably the alignment system comprises one or more alignment elements, wherein at least one alignment element of the one or more alignment elements is preferably assigned to each interface element of the one or more interface elements. By assigning an alignment element to each interface element of the one or more interface elements also multiple bioprocess components can be aligned and preferably retained individually, thus simplifying an individual setup of bioprocess components on the bioprocessing base. The alignment system is preferably designed as one or more spring-loaded bolts, one or more guiderails and / or one or more retaining clips. In this case, preferably, the one or more alignment elements are designed as at least one spring-loaded bolts of the one or more spring-loaded bolts, at least one guide rail of the or more guiderails and / or at least one retaining clip of the one or more retaining clips. The one or more guiderails provide a constructively simple design for aligning and, preferably, at least partially fixing the bioprocess component. The one or more spring-loaded bolts provide a secure alignment and, preferably, at least partial fixation of the bioprocess component. Due to the spring-loading the alignment and preferably fixation can also be ensured without a power supply.

[0024] In an embodiment of the bioprocessing base it is provided that the adapter module further comprises at least one pressure sensor for measuring the liquid pressure inside a tube section, wherein preferably the pressure sensor is a piezo-resistive pressure sensor. The pressure sensor, in particular the information derived from the pressure sensor, can be used to precisely control and / or monitor the conveying of liquid in a tube, preferably of a tube set of the bioprocess component, and / or by the pumping means. By using a piezo-resistive pressure sensor very small and / or quick changes in pressure can be detected, making it particularly suitable for the use in a bioprocess.

[0025] In an embodiment of the bioprocessing base it is provided that the adapter module is reversibly electrically connectable to the one or more interface elements, and / or that the adapter module is reversibly mountable on the base body. In this way the adapter module can be removed, for example for maintenance and / or cleaning, and / or swapped to replace and / or upgrade the adapter module.

[0026] In an embodiment of the bioprocessing base it is provided that the bioprocessing base comprises exactly two interface elements. This is a good compromise between being able to perform different operations, preferably unit operations, of a bioprocess on the bioprocessing base and a compact design, which comes in handy when swapping the bioprocessing base.

[0027] The disclosure also concerns a bioprocessing station, which bioprocessing station comprises a bioprocessing base according to any one of claims 1 to 8 and at least one bioprocess component. The technical effects explained before with regard to the bioprocessing base and / or the bioprocess component apply correspondingly here. The bioprocess component can be used to perform one or more operations, preferably unit operations, of a bioprocess. It can also be provided that the bioprocessing station comprises multiple bioprocess components. By providing multiple bioprocess components different consumable elements can easily be provided on the bioprocessing base and / or different operations, preferably unit operations, of a bioprocess can be performed. The bioprocessing station can thus be easily modified for different operations, preferably unit operations, of a bioprocess and / or for different bioprocesses very individually. The one or more bioprocess components are preferably detachably connected to the bioprocessing base and / or movable relative to the bioprocessing base at least when the interface alignment means, preferably the magnetic retention system, is in the release state and / or the locking means is in the open position.

[0028] In an embodiment of the bioprocessing station it is provided that the bioprocess component comprises a docking element in the form of a tray or a cartridge, wherein the tray is configured to dock onto the bioprocessing base by interfacing with at least one interface element of the bioprocessing base, and wherein the cartridge is configured to dock onto the bioprocessing base by interfacing with the adapter module of the bioprocessing base. Thus, differently designed bioprocess components for different proposes are provided. In case the bioprocess component comprises a docking element in the form of a tray, the bioprocess component does not need to interface with the adapter module to dock onto the bioprocessing base, thus simplifying it to provide multiple bioprocess components on the bioprocessing base, as interfacing every bioprocess component to the adapter module can be difficult to achieve. Moreover, this design is in particular advantageous when a bioprocess component is simply used as a storage compartment for a consumable element, which consumable element is for example not intended for connection to another bioprocessing base via the adapter module. In case the bioprocess component comprises a docking element in the form of a cartridge, by interfacing with the adapter module a direct connection between the bioprocess component and the bioprocessing base, preferably adapter module, is provided via which a direct fluidic, electric and / or mechanical connection is provided, for example a fluidic connection via the fluidic valve actuators, an electric connection via the electrical connector and / or a mechanical connection via the locking means. The cartridge is preferably configured to interface with at least one, preferably at least two, interface element of the one or more interface elements. In this way more space is provided in the cartridge for consumable elements. The tray is preferably configured to interface with at least one, preferably only one, interface elements of the one or more interface elements. In this way multiple trays can be arranged on the bioprocessing base and / or the trays can be arranged individually on the bioprocessing base, preferably one or more interface elements, thus simplifying an individual set-up on the bioprocessing base.

[0029] In an embodiment of the bioprocessing station it is provided that the cartridge comprises: at least one bioprocess container configured to hold a liquid for use in a bioprocess, a tube set configured to be fluidically connectable to the adapter module of the bioprocessing base and / or to the at least one bioprocess container, for example by being weldable and cuttable. This simplifies performing a bioprocess with the bioprocess component comprising a cartridge. By providing the tube set to be weldable and cuttable a fluid-tight and / or aseptic connection can be more easily established via welding, preferably via welding at least one tube of the tube set to another tube of preferably another tube set, and / or suspended via cutting, preferably of a tube of the tube set. The tube set is preferably fixedly and / or reversibly connectable, preferably connected, to the adapter module of the bioprocessing base and / or to the at least one bioprocess container. In this way a secure and preferably also detachable connection is given. Alternatively or in addition it can also be provided that the tube set is, preferably reversibly, connectable to another bioprocess component, preferably to the tube set and / or bioprocess container of another bioprocess component. In this way fluid can easily be transferred between the bioprocess components. The tube set is preferably made at least in sections from plastic, as this material is easy to weld and cut. The bioprocess container can comprise a, preferably single-use, bag, preferably transfer bag. Alternatively or in addition, the bioprocess container and / or the tube set can respectively be a, preferably single-use, consumable element. This further increases the handling, as no cumbersome cleaning of these element is necessary.

[0030] In addition, it is provided that the cartridge comprises at least one tube holder block, preferably comprising, as a bioprocess container, a tube. The tube holder block simplifies welding of a tube with an at least partially, preferably fully, automated welding device, as the tube holder blocks provide defined reference elements for the welding device at which reference elements the welding device can orientate during the welding process. Alternatively or in addition the tray can also comprise at least one tube holder block, preferably comprising, as a bioprocess container, a tube.

[0031] Optionally it is provided that the at least one bioprocess container comprises a low volume transfer vessel and / or a transfer bag. The low volume transfer vessel is preferably a rigid vessel and / or a vessel containing less than 100 milliliters, preferably less than 50 milliliters, in particular less than 25 milliliters. This design allows for liquid to be removed from the low volume transfer vessel with minimal residue remaining behind, simplifying the conveying of a very precise amount of liquid. Alternatively or in addition, the low volume transfer vessel can comprise a filter, preferably in a lid of the low volume transfer vessel, to allow air to enter and / or exit the low volume transfer vessel, thus helping to avoid the pressurization and / or creation of a vacuum as the liquid level changes in the low volume transfer vessel. The transfer bag is preferably an expandable bag. The low volume transfer vessel and / or the transfer bag are respectively configured for storing liquid to be transferred to and / or from another vessel, preferably via the tube set.

[0032] In an embodiment of the bioprocessing station it is provided that the bioprocessing base is configured to provide an electrical connection to the bioprocess component. In this way the bioprocess component can be powered without the need for an own power source. The electrical connection can be provided as a wired connection and / or wireless connection, for example via induction. The disclosure also concerns a bioprocessing system comprising a housing and at least two bioprocessing bases according to any one of claims 1 to 8, preferably at least two bioprocessing stations according to any one of claims 9 to 12.

[0033] The technical effects explained before with regard to the bioprocessing base and / or the bioprocessing station apply correspondingly here. By providing at least two bioprocessing bases, preferably bioprocessing stations, the bioprocessing system can more easily be adapted for different bioprocesses and / or operations, preferably unit operations, of a bioprocess. The housing provides stability and / or protection. The housing can at least partially encompass the at least two bioprocessing stations.

[0034] In an embodiment of the bioprocessing system it is provided that the housing comprises one or more guiding structures adapted to receive and position the base alignment means of the bioprocessing bases, preferably bioprocessing stations. This simplifies optimally positioning of the bioprocessing bases, preferably bioprocessing stations, within the bioprocessing system. The one or more guiding structures can be designed as, preferably oppositely arranged, guiding rails for respectively guiding the bioprocessing bases, preferably bioprocessing stations.

[0035] In addition, the one or more guiding structures are arranged to allow the bioprocessing bases, preferably bioprocessing stations, to be slid into processing positions which are defined relative to one another, wherein preferably the bioprocessing bases, preferably bioprocessing stations, are positioned side-by-side. In this way the bioprocessing bases, preferably bioprocessing stations, can be easily moved in and also out of the housing of the bioprocessing system. In this context it is preferably provided that the bioprocessing bases, preferably bioprocessing stations, are movable, preferably slidable, between the respective processing position and a respective release position arranged outside the one or more guiding structures and / or the housing of the bioprocessing system. The processing positions are preferably arranged at least in sections between the one or more guiding structures and / or at least in sections inside the housing. Alternatively or in addition the processing positions are preferably intended for the performance of at least one operation, preferably unit operation, of a bioprocess on at least one of the bioprocessing bases, preferably at least one of the bioprocessing stations, and / or a welding step, preferably on at least one tube set of at least one of the bioprocessing stations and / or by means of a welding device of the bioprocessing system. The at least one operation, preferably unit operation, of the bioprocess can be performed using the respective bioprocessing base, preferably the respective bioprocessing station. For example, at least one interface element of the one or more interface elements and / or the pumping means of at least one of the bioprocessing bases, preferably bioprocessing stations, can be used for performing the operation, preferably unit operation, of the bioprocess in the processing position. Alternative or in addition an active element of the bioprocessing system, preferably movable along at least one, preferably at least three, rotational degrees of freedom and / or along at least one, preferably at least three, translational degrees of freedom, can be used to perform the at least one operation, preferably unit operation, of the bioprocess in the processing position. The active element can be movable relative to the housing of the bioprocessing system, the one or more guiding structures and / or the processing positions. By a side-by-side arrangement fluidical connections between different bioprocessing bases, preferably bioprocessing stations, can be easily established, in particular when using an at least partially, preferably fully, automated welding device.

[0036] In addition, it is provided that the housing comprises one or more alignment features configured to ensure accurate positioning of the bioprocessing bases, preferably bioprocessing stations, within the bioprocessing system. In this way the bioprocessing bases, preferably bioprocessing stations, can be easily arranged in a position where the bioprocessing base, preferably bioprocessing station, can be used to perform one or more operations, preferably unit operations, of a bioprocess and / or the bioprocess as intended. The one or more alignment features can for example comprise an indicator means for indicating an intended position of the respective bioprocessing base, preferably bioprocessing station, and / or an end stop to define an intended position of the respective bioprocessing base, preferably bioprocessing station. In an embodiment of the bioprocessing system it is provided that the bioprocessing system comprises docking means configured to form a connection with a bioprocessing base, preferably bioprocessing station, arranged in a processing position, wherein preferably the connection is formed with the adapter module and / or base body of the bioprocessing base. In this way a dedicated means is provided to connect a bioprocessing base, preferably bioprocessing station, to the rest of the bioprocessing system, thus further improving the handling of the different parts of the bioprocessing system. The connection means formed via the docking means is preferably a detachable connection, thus simplifying swapping or changing bioprocessing bases, preferably bioprocessing stations. Alternatively or in addition via the docking means a fluidic, electric and / or mechanical connection can be provided, preferably to the rest of the bioprocessing system.

[0037] In an embodiment of the bioprocessing system it is provided that at least one, preferably each, bioprocessing base, preferably bioprocessing station, when positioned in a processing position, is supported, preferably exclusively, by the one or more guiding structures, such that the respective bioprocessing base, preferably bioprocessing station, remains elevated above a, preferably any, underlying surface. On the one hand, this simplifies cleaning as this design provides at least a gap underneath the bioprocessing bases, preferably bioprocessing stations, to clean any spillage. On the other hand, this simplifies using modified, for example upgraded, bioprocessing bases, preferably bioprocessing stations, with in the same housing without the need to substantially or even at all change the housing of the bioprocessing system. Modifications can be made such that the lower part of the respective bioprocessing base, preferably bioprocessing station, is altered, for example extended downwards, without inevitably leading to a collision with the existing housing.

[0038] In an embodiment of the bioprocessing system it is provided that the bioprocessing system comprises means to identify at least one, preferably each, of the bioprocessing bases, preferably bioprocessing stations, preferably to identify a bioprocess component connected to the bioprocessing bases. This can be used on the one hand to avoid mix-ups of bioprocessing bases, preferably bioprocessing stations, which could lead to an operation, preferably unit operation, of a bioprocess and / or welding process being performed on the wrong bioprocessing base, preferably bioprocessing station. This can also be used for determining if a bioprocessing base, preferably bioprocessing station, is arranged in the intended processing position, means to identify at least one, preferably each, of the bioprocessing bases can preferably comprise a, preferably optical, scanner and / or be configured to read a machine readable medium, preferably a barcode, NFC-tag and / or RFID-tag.

[0039] In an embodiment of the bioprocessing system it is provided that the bioprocessing system is set up to generate a, preferably reversible, fluid-tight connection between adjacent bioprocessing bases, preferably bioprocessing stations, wherein preferably the, preferably reversible, fluid-tight connection is established through one or more consumable elements which may comprise a tube set and / or a bioprocess container. Thus, different bioprocessing bases, preferably bioprocessing stations, can be connected to transfer fluids, preferably liquids, when performing a bioprocess and / or an operation, preferably unit operation, of a bioprocess. The reversible fluid-tight connection is preferably generated by the welding device of the bioprocessing system and / or by welding together a consumable element, preferably a tube set and / or bioprocess container of a consumable element, of one of the bioprocessing stations to a consumable element, preferably a tube set and / or bioprocess container of a consumable element, of another of the bioprocessing stations. Using the welding device, which is preferably at least partially, preferably fully, automated, on the one hand reduces the risk of contamination as user interaction is limited or even not necessary at all. On the other hand, this can reduce the process time of a bioprocess, as a user can simultaneously perform other tasks in conjunction with the bioprocess when the welding device is welding. Using consumable elements for establishing the connection reduces the up the set-up time of a bioprocess, as no multi-use elements need to be used which are cumbersome and time-consuming to clean. The consumable elements can instead be discarded after the use and thus no or only little cleaning is necessary before establishing the fluid connection.

[0040] The disclosure also concerns the use of a bioprocess component in combination with a bioprocessing base according to any one of claims 1 to 8 for performing a bioprocess, preferably of a bioprocessing station according to any one of claims 9 to 12 for performing a bioprocess. The technical effects explained before with regard to the bioprocessing base, the bioprocess component and the bioprocessing station apply correspondingly here. The bioprocess component can preferably be configured as described beforehand in conjunction with the bioprocessing base, the bioprocessing station and / or the bioprocessing system.

[0041] In an embodiment of the use it is provided that the bioprocess component comprises at least one tray configured to dock onto the bioprocessing base by interfacing with at least one interface element of the bioprocessing base. The tray is preferably the docking element in the form of a tray. By using a respectively designed bioprocess component, the bioprocess component does not need to interface with the adapter module to dock onto the bioprocessing base, thus simplifying providing multiple bioprocess components on the bioprocessing base, as interfacing every bioprocess component to the adapter module can be difficult to achieve. Moreover, this design is in particular advantageous when a bioprocess component is simply used as a storage compartment for a consumable element, which consumable element is not intended to be connected to another bioprocessing base via the adapter module.

[0042] In an embodiment of the use it is provided that the bioprocess component comprises a cartridge configured to dock onto the bioprocessing base by interfacing with the adapter module of the bioprocessing base. The cartridge is preferably the docking element in the form of a cartridge. By using a respectively designed bioprocess component, by interfacing with the adapter module a direct connection between the bioprocess component and the bioprocessing base, preferably adapter module, is provided via which a direct fluidic, electric and / or mechanical connection is provided, for example a fluidic connection via the fluidic valve actuators, an electric connection via the electrical connector and / or a mechanical connection via the locking means.

[0043] The disclosure also concerns a method of processing a biological sample in a bioprocessing system according to any one of claims 13 to 18, the method comprising: arranging at least two bioprocessing stations in adjacent processing positions. This simplifies conveying fluids, preferably liquids, between the bioprocessing stations as connections can be made over short distances and preferably without complex conduit systems.

[0044] In addition, the method comprises generating a fluid-tight connection between the bioprocessing stations, preferably by welding, wherein preferably the reversible fluid- tight connection is established through one or more consumable elements which may comprise a tube set and / or a bioprocess container. Thus, different bioprocessing stations can be connected to transfer fluids, preferably liquids, when performing a bioprocess and / or an operation, preferably unit operation, of a bioprocess. The reversible fluid-tight connection is preferably generated by the welding device of the bioprocessing system and / or by welding together a consumable element, preferably a tube set and / or bioprocess container of a consumable element, of one of the bioprocessing stations to a consumable element, preferably a tube set and / or bioprocess container of a consumable element, of another of the bioprocessing stations. Using the welding device, which is preferably at least partially, preferably fully, automated, on the one hand reduces the risk of contamination as user interaction is limited or even not necessary at all. On the other hand, this can reduce the process time of a bioprocess, as a user can simultaneously perform other tasks in conjunction with the bioprocess when the welding device is welding. Using consumable elements for establishing the connection reduces the up the set-up time of a bioprocess, as no multi-use elements need to be used which are cumbersome and time-consuming to clean. The consumable elements can instead be discarded after the use and thus no or only little cleaning is necessary before establishing the fluid connection. The one or one or more consumable elements can preferably be the one or one or more consumable elements described before in conjunction with the bioprocessing system.

[0045] In addition, the method comprises performing a bioprocess. The bioprocess preferably comprises one or more operations, preferably unit operations and / or the bioprocess can be for cell and / or gene therapy production.

[0046] In addition, the method optionally comprises severing the fluid-tight connection, preferably by sealing and cutting. In this way the bioprocessing stations of the bioprocessing system can be disconnected from one another and preferably at least one of the bioprocessing stations can be removed from the housing of the bioprocessing system and / or be connected, preferably via a fluid-tight connection, to another bioprocessing station. Sealing helps reducing the risk of contamination and is preferably performed before cutting, thus further reducing the risk of contamination.

[0047] In an embodiment of the method it is provided that the performing of the bioprocess involves transferring fluid from one bioprocessing station to another bioprocessing station. This can for example be used for dilution or seeding by transferring cells. However other operations, preferably unit operations, of the bioprocess are also conceivable.

[0048] Further features and advantages of the bioprocessing base, the bioprocessing station, the bioprocessing system, the use of a bioprocess component with a bioprocessing base and the method of processing a biological sample in a bioprocessing system emerge from the following description of exemplary embodiments where reference is made to the attached drawing.

[0049] In the drawing

[0050] Fig. 1 shows an exemplary embodiment of a bioprocessing base in a perspective view, Fig. 2 shows the bioprocessing base according to Fig. 1 in a top view,

[0051] Fig. 3 shows an exemplary embodiment of a bioprocessing station in a perspective view,

[0052] Fig. 4 shows an exemplary embodiment of a bioprocessing system in a perspective view and

[0053] Fig. 5 shows a sectional view of the bioprocessing system according to Fig. 4.

[0054] Fig. 1 shows an exemplary embodiment of a bioprocessing base 1 in a perspective view. The bioprocessing base 1 comprises a base body 2, which base body comprises base alignment means 3 and interface elements 4, in the shown embodiment two interface elements 4, adapted to support and preferably accommodate a bioprocess component 18. The base body 2 also comprises a frame 5. In the shown embodiment the base alignment means 3 are formed as oppositely arranged protruding edges of the frame 5. The interface elements 4 are also connected to the frame 5.

[0055] In the shown embodiment the interface elements 4 respectively comprise means for determining the weight of the bioprocess component 6 and means for agitating the bioprocess component 7 to be supported by the interface elements 4. Here the means for determining the weight of the bioprocess component 6 is respectively a load cell and the means for agitating the bioprocess component 7 is respectively a magnetic device for employing a rotating magnetic field. The magnetic device can thus be used to rotate a stir bar provided in the bioprocess component 18. However, it is also conceivable that the means for agitating the bioprocess component 7 are dispensed with. It is also conceivable that the interface elements 4 respectively comprise a means for controlling and adjusting the temperature of the bioprocess component. In the shown embodiment the interface elements 4 also respectively comprise interface alignment means 13 for positioning and optionally retaining the bioprocess component 18 on the respective interface element 4 on which the bioprocess component 18 is supported. The interface alignment means 13 of the embodiment shown is a magnetic retention system comprising permanent magnets and being adjustable between a retention state for positioning and preferably retaining the bioprocess component 18 via magnetic force and a release state for releasing the bioprocess component 18.

[0056] In addition, the bioprocessing base 1, in particular the base body 2, also comprises a pumping means 8 which is in the shown embodiment a peristaltic pump head. This can be used to convey liquid provided in a bioprocess component 18 to be supported by at least one of the interface elements 4.

[0057] Moreover, the shown bioprocessing base 1 comprises an adapter module 9. The adapter module 9 in turn comprises multiple fluidic valve actuators 10. Moreover, in the shown embodiment an electrical connector 11 is also arranged on the adapter module 9. However, the electrical connector 11 can also be arranged on the base body 2 or even be dispensed with. The fluidic valve actuators 10 are configured for interacting with a tube 25 and / or multifluidic cassette, preferably of a tube set 24 of the bioprocess component 18 to be supported on at least one of the interface elements 4. Via the electrical connector 11 the bioprocessing base 1 can be supplied with power. The electrical connector 11 in the shown embodiment is arranged on the back of the adapter module 9, however, the electrical connector 11 could also be placed at another position, for example at the base body 2.

[0058] The adapter module 9 in the shown embodiment also comprises locking means 12 for reversibly mounting the bioprocess component 18 at a fixed position. Thus, the bioprocess component 18 can be securely fixed in particular while performing a bioprocess. The locking means 12 of the shown embodiment are a pair of cam locks, which cam locks are spaced apart from another. The locking means 12 can be adjusted between a, preferably normally, closed position for mounting the bioprocess component 18 at the fixed position and an open position for releasing the bioprocess component 18.

[0059] The adapter module 9 of the shown embodiment further comprises two pressure sensors 16, which are arranged inside of the adapter module 9 and respectively designed as a piezo-resistive pressure sensor, for respectively measuring the liquid pressure inside a tube section of a tube 25. The tube section is in particular the tube section of a tube 25 of a tube set 24 of a bioprocess component 18. It is also conceivable to provide more or fewer pressure sensors 16.

[0060] The adapter module 9 is also reversibly electrically connectable to the interface elements 4, in particular to power the means for determining the weight of the bioprocess component 6 and the means for agitating the bioprocess component 7. In case a means for controlling and adjusting the temperature of the bioprocess component is provided, this means can also be powered in this way.

[0061] Moreover, the adapter module 9 is reversibly mountable to the base body 2, thus simplifying swapping of the adapter module 9. However, this may not be the case. Alternatively the adapter module 9 can be fixedly mounted to the base body 2.

[0062] In order to further simplify alignment and fixation of the bioprocess components 18 to be supported by the bioprocessing base 1, the bioprocessing base 1 also comprises an alignment system for aligning the bioprocess component with the bioprocessing base 1 and reversibly fixing it thereto, i.e. to the bioprocessing base 1. In the embodiment shown the alignment system comprises different alignment elements. Some of the alignment elements are provided in the form of spring-loaded bolts 14, which can interact with, preferably the underside of, a bioprocess component 18. Some of the alignment elements are provided as guiderails 15 which can interact with a ridge and / or slot of a bioprocess component 18. Moreover, it also conceivable that alignment system comprises one or more retaining clips. Fig. 2 shows the bioprocessing base 1 according to Fig. 1 in a top view. Here in particular the electrical connector 11 of the adapter module 9 can be clearly seen. However, the electrical connector 11 could also be provided at the base body 2.

[0063] Fig. 3 shows an exemplary embodiment of a bioprocessing station 17 in a perspective view. The bioprocessing station 17 comprises the bioprocessing base 1 according to Fig. 1 and a bioprocess component 18. For the sake of clarity the bioprocess component 18 is arranged spaced a part from the bioprocessing base 1, however, the bioprocess component 18 can be moved and docked onto the bioprocessing base 1.

[0064] In the shown embodiment the bioprocess component 18 comprises a docking element 19 in the form of a cartridge 20, wherein the cartridge 20 is configured to dock onto the bioprocessing base 1 by interfacing with the adapter module 9 of the bioprocessing base 1. The docking is here provided by the locking means 12 of the adapter module 9, which interact with recesses 21 of the cartridge 20 (However, only one recess 21 can be clearly seen in Fig. 3).

[0065] The cartridge 20 comprises two bioprocess containers 22 to hold a liquid for use in a bioprocess. One of the bioprocess containers 22 is designed as a low volume transfer vessel 23 and the other container 22 is designed as a transfer bag 36. In addition, the cartridge comprises a tube set 24 (For the sake of clarity only parts of the tube set 24 are shown in Fig. 3). The tube set 24 will be reversibly fluidically connected to the adapter module 9 of the bioprocessing base 1, to allow conveying liquid via the adapter module 9, in particular via the multiple fluidic valve actuators 10, using the pumping means 8. The tube set 24 is also fluidically connected to the bioprocess containers 22, i.e. the transfer bag 36 and the low volume transfer vessel 23. The tube set 24 in the shown embodiment is fixedly connected to the bioprocess containers 22 and can preferably be reversibly connected to a tube set 24 of another bioprocess component 18, for example another bioprocess component 18 comprising a tray 42. The fluidically connections, in particular to the transfer bag 36 and the low volume transfer vessel 23, are fluid-tight. The tubes 25 of the tube set 24 can be held by the tube holder blocks 26 and thus are held in a defined position which facilitates welding of these tubes 25 by an automated welding device 39, for example to be connected to a tube set 25 of another bioprocess component 18. In order to disconnect these, preferably welded, connections, the tubes 25 of the tube set 24 can be severed by cutting. Before the disconnection it is, however, advantageous that the tubes 25 of the tube set 24 are sealed in order to avoid contamination.

[0066] When the bioprocess component 18 is docked to the bioprocessing base 1 the bioprocessing base 1 can provide an electrical connection to bioprocess component 18, for example via induction and / or a wired connection. However, this does not have to be the case.

[0067] Alternatively or in addition however, the bioprocess component 18 could comprise a docking element 19 in the form of a tray 42. The tray 42 can be designed similarly to a cartridge 20. However, the tray 42 is configured to dock onto the bioprocessing base 1 by interfacing with at least one interface element 4 of the bioprocessing base 1, in particular via the interface alignment means 13. The tray does preferably not interface with the adapter module 9 and / or only interfaces with one of the interface elements 4, thus allowing for a very individual arrangement on the bioprocessing base 1, in particular the interface elements 4, and / or the arrangement of multiple trays 42.

[0068] Fig. 4 shows an exemplary embodiment of a bioprocessing system 27 in a perspective view. The bioprocessing system 27 comprises a housing 28 as well as three bioprocessing stations 17.

[0069] For controlling and / or monitoring a bioprocess and / or the bioprocessing stations 17 the bioprocessing system 1 comprises a control device 35.

[0070] Moreover, in the shown embodiment the bioprocessing system 1 also comprises multiple mobile bioprocessing modules 29, which are, however, not a mandatory part of the bioprocessing system 1. The mobile bioprocessing modules 29 respectively comprise a bioreactor, in particular used for cell expansion. However, the mobile bioprocessing modules 29 can also be used for other operations of a bioprocess. The mobile bioprocessing modules 29 can be stored in storage locations 30, which storage locations 30 are stacked on top of each other in the shown embodiment. In order to move the mobile bioprocessing modules 29 into a workstation 31, a handling device

[0071] 32 is provided, which handling device 32 can in particular move the mobile bioprocessing modules 29 up and down and / or move the mobile bioprocessing modules 29 out of and / or into the storage locations 30.

[0072] The housing 28 of the bioprocessing system 27 comprises several guiding structures

[0073] 33 for receiving and positioning the base alignment means 3 of the bioprocessing bases 1. In the embodiment shown the guiding structures 33 are designed as guiding rails. The guiding structures 33 allow the bioprocessing bases 1 together with the bioprocess components 18 (i.e. the bioprocessing stations 17) to be slid easily into processing positions 34 (the processing positions 34 are referenced in Fig. 5 for the sake of clarity) which are positioned side-by-side such that the bioprocessing stations 17 are positioned side-by-side.

[0074] When positioned in the processing position 34, the bioprocessing bases 1 and in the embodiment shown thus also the entire bioprocessing stations 17 are supported by the one or more guiding structures 33, such that the bioprocessing bases 1 are elevated above any underlying surface 37. Thus, a clearance underneath the bioprocessing bases 1 is given, which simplifies cleaning and also allows for an extension of the bioprocessing bases 1 downwards.

[0075] The bioprocessing system 1 also comprises in the shown embodiment means to identify the bioprocessing bases 1, preferably bioprocessing stations 17, and / or bioprocess components 18 connected to the bioprocessing bases 1. The means to identify are in the shown embodiment scanners 38. These scanners 38 can for example scan, preferably unique, identifiers, for example barcodes, on the bioprocessing bases 1 and / or the bioprocess components 18 for the identification.

[0076] The bioprocessing system 1 is also set up to generate a, preferably reversible, fluid- tight connection between adjacent bioprocessing bases 1, preferably bioprocessing stations 27. The fluid-tight connection is established in particular through the tube sets 24 of the bioprocessing stations 17. As show in the detail of Fig. 4 the tube sets 24 of two adjacent cartridges 20 are welded together, in particular the tubes 25 of the respective tube sets 24 were welded together between a tube holder blocks 26 of each cartridge 20. The welding was performed by the, preferably automated, welding device 39 of the bioprocessing system 1. This fluid-tight connection can then be used to transfer fluid from one bioprocessing station 17 to another bioprocessing station 17, preferably during a bioprocess.

[0077] In the shown embodiment the welding device 39 can be moved along three translational axis and can thus easily reach the different bioprocessing stations 17 and the mobile bioprocessing module 29 arranged in the workstation 31. When the connection shall be severed, the welding device 39 cuts the tubes 25 of the tube sets 24. Before the cutting, however, the tubes 25 of the tube sets 24 are sealed by the welding device 39 to avoid contamination. For cutting the tubes 25 the welding device 39 comprises at least one detachable and / or disposable knife. In order to store the one or more knives to be picked up by the welding device 39 for cutting and / or to store used knives to be discarded, the shown embodiment also comprises a knife storing module 41 arranged in the workstation 31.

[0078] The welding device 39 is coupled in the shown embodiment with an active element 40, which active element 40 can thus move with the welding device 39. This active element 40 can interact with the bioprocessing stations 17 to perform an operation, preferably unit operation, of a bioprocess and / or to interact in a bioprocess. For example, the active element 40 can comprise a pump or an element to transfer energy to the bioprocessing stations 17. However, an active element 40 does not need to be provided. Moreover, it is also conceivable that the welding device 39 can move at least one tube 25, preferably of the tube set 24, of the bioprocess component 18 and / or bioprocessing station 17, forwards and / or backwards. This can be used to align the respective tube 25 before welding.

[0079] When an intended operation, preferably unit operation, of a bioprocess and / or a bioprocess was performed on a bioprocessing station 17, the respective bioprocess component 18 can be removed from the respective processing position 34 and / or bioprocessing base 1 and out of the housing 28.

[0080] Fig. 5 shows a sectional view of the bioprocessing system 1 according to Fig. 4. The sectional view runs along the cut V-V in Fig. 4.

[0081] In Fig. 5 the bioprocessing bases 1 have a different sets of bioprocess components 18 than in Fig. 4. The right hand side bioprocessing base 1 does not comprise any bioprocess components 18 at all, thus both interface elements 4 are free. On the middle bioprocessing base 1 a cartridge 20 is arranged, which cartridge interacts with the adapter module 9 of the respective bioprocessing base 1 and covers both active element 4. On the left hand side bioprocessing base 1 a single tray 42 is arranged, which covers and interacts with only one interface element 4, here the frontal interface element 4.

[0082] For forming a connection with the bioprocessing bases 1, preferably bioprocessing stations 17, arranged in the processing positions 34 the bioprocessing system 1 comprises docking means 43. The respective connection is formed between the docking means 43 and the bioprocessing base 1 of the respective bioprocessing base 1.

[0083] Moreover, the housing 28 comprises an alignment feature 44 to ensure accurate positioning of the bioprocessing bases 1, preferably bioprocessing stations 17, within the bioprocessing system 1. In the embodiment shown the alignment feature 44 is designed as an end stop.

[0084] List of reference signs

[0085] 1 Bioprocessing base

[0086] 2 Base body

[0087] 3 Base alignment means

[0088] 4 Interface element

[0089] 5 Frame

[0090] 6 Means for determining the weight of the bioprocess component

[0091] 7 Means for agitating the bioprocess component

[0092] 8 Pumping means

[0093] 9 Adapter module

[0094] 10 Fluidic valve actuator

[0095] 11 Electrical connector

[0096] 12 Locking means

[0097] 13 Interface alignment means

[0098] 14 Spring-loaded bolt

[0099] 15 Guiderail

[0100] 16 Pressure sensor

[0101] 17 Bioprocessing station

[0102] 18 Bioprocess component

[0103] 19 Docking element

[0104] 20 Cartridge

[0105] 21 Recess

[0106] 22 Bioprocess container

[0107] 23 Low volume transfer vessel

[0108] 24 Tube set

[0109] 25 Tube

[0110] 26 Tube holder block

[0111] 27 Bioprocessing system 28 Housing

[0112] 29 Mobile bioprocessing module

[0113] 30 Storage location

[0114] 31 Workstation

[0115] 32 Handling device

[0116] 33 Guiding structure

[0117] 34 Processing position

[0118] 35 Control device

[0119] 36 Transfer bag

[0120] 37 Underlying surface

[0121] 38 Scanner

[0122] 39 Welding device

[0123] 40 Active element

[0124] 41 Knife storing module

[0125] 42 Tray

[0126] 43 Docking means

[0127] 44 Alignment feature

Claims

C l a i m s1. Bioprocessing base (1) for performing a bioprocess, comprising: a base body (2) comprising: base alignment means (3), one or more interface elements (4) adapted to support and preferably accommodate a bioprocess component (18), and pumping means (8), preferably a peristaltic pump head; an adapter module (9) comprising: one or more fluidic valve actuators (10); and optionally at least one electrical connector (11).

2. Bioprocessing base (1) according to claim 1, wherein the adapter module further comprises locking means (12) for reversibly mounting the bioprocess component (18) at a fixed position, such as a pair of cam locks.

3. Bioprocessing base (1) according to claim 1 or 2, wherein at least one, preferably each, of the interface elements (4) comprises means for determining the weight of the bioprocess component (6), means for controlling and adjusting the temperature of the bioprocess component, and / or means for agitating the bioprocess component (7).

4. Bioprocessing base (1) according to any one of the preceding claims, wherein at least one, preferably each, of the interface elements (4) comprises interface alignment (13) means for positioning and optionally retaining the bioprocess component (18), such as a magnetic retention system.

5. Bioprocessing base (1) according to any one of the preceding claims, comprising an alignment system for aligning the bioprocess component with the bioprocessing base (1) and, preferably, reversibly fixing it thereto, for example one or more spring-loaded bolts (14) and / or one or more guiderails (15) and / or one or more retaining clips.

6. Bioprocessing base (1) according to any one of the preceding claims, wherein the adapter module (9) further comprises at least one pressure sensor (16) for measuring the liquid pressure inside a tube section, wherein preferably the pressure sensor is a piezo-resistive pressure sensor.

7. Bioprocessing base (1) according to any one of the preceding claims, wherein the adapter module (9) is reversibly electrically connectable to the one or more interface elements (4), and / or wherein the adapter module (9) is reversibly mountable on the base body (2).

8. Bioprocessing base (1) according to any one of the preceding claims comprising exactly two interface elements (4).

9. Bioprocessing station (17) comprising a bioprocessing base (1) according to any one of claims 1 to 8 and at least one bioprocess component (18).

10. Bioprocessing station (17) according to claim 9, wherein the bioprocess component (18) comprises a docking element (19) in the form of a tray (42) or a cartridge (20), wherein the tray (42) is configured to dock onto the bioprocessing base (1) by interfacing with at least one interface element (4) of the bioprocessing base (1), and wherein the cartridge (20) is configured to dock onto the bioprocessing base (1) by interfacing with the adapter module (9) of the bioprocessing base (1).

11. Bioprocessing station (17) according to claim 10, wherein the cartridge (20) comprises: at least one bioprocess container (22) configured to hold a liquid for use in a bioprocess, a tube set (24) configured to be fluidically connectable to the adapter module (9) of the bioprocessing base (1) and / or to the at least one bioprocess container (22), for example by being weldable and cuttable, optionally at least one tube holder block (26), preferably comprising, as a bioprocess container (22), a tube; optionally wherein the at least one bioprocess container (22) comprises a low volume transfer vessel (23) and / or a transfer bag (36).

12. Bioprocessing station (17) according to any one of claims 9 to 11, wherein the bioprocessing base (1) is configured to provide an electrical connection to the bioprocess component (18).

13. Bioprocessing system (27) comprising a housing (28) and at least two bioprocessing bases (1) according to any one of claims 1 to 8, preferably at least two bioprocessing stations (17) according to any one of claims 9 to 12.

14. Bioprocessing system (27) according to claim 13, wherein the housing (28) comprises one or more guiding structures (33) adapted to receive and position the base alignment means (3) of the bioprocessing bases (1), preferably bioprocessing stations (17); wherein the one or more guiding structures (33) are arranged to allow the bioprocessing bases (1), preferably bioprocessing stations (17), to be slid into processing positions (34) which are defined relative to one another, wherein preferably the bioprocessing bases (1), preferably bioprocessing stations (17), are positioned side-by-side; and wherein the housing (28) comprises one or more alignment features (44) configured to ensure accurate positioning of the bioprocessing bases (1), preferably bioprocessing stations (17), within the bioprocessing system (27).

15. Bioprocessing system (27) according to claim 13 or 14, further comprising docking means (43) configured to form a connection with a bioprocessing base (1), preferably bioprocessing station (17), arranged in a processing position (34), wherein preferably the connection is formed with the adapter module (9) and / or base body (2) of the bioprocessing base (1).

16. Bioprocessing system (27) according to any one of claims 13 to 15, wherein at least one, preferably each, bioprocessing base (1), preferably bioprocessing station (17), when positioned in a processing position (34), is supported, preferably exclusively, by the one or more guiding structures (33), such that the respective bioprocessing base (1), preferably bioprocessing station (17), remains elevated above a, preferably any, underlying surface (37).

17. Bioprocessing system (27) according to any one of claims 13 to 16 further comprising means to identify at least one, preferably each, of the bioprocessing bases (1), preferably bioprocessing stations (17), preferably to identify a bioprocess component (18) connected to the bioprocessing bases (1).

18. Bioprocessing system (27) according to any one of claims 13 to 17, wherein the bioprocessing system (27) is set up to generate a, preferably reversible, fluid- tight connection between adjacent bioprocessing bases (1), preferably bioprocessing stations (17), wherein preferably the, preferably reversible, fluid- tight connection is established through one or more consumable elements which may comprise a tube set (24) and / or a bioprocess container (22).

19. Use of a bioprocess component (18) in combination with a bioprocessing base (1) according to any one of claims 1 to 8 for performing a bioprocess, preferably of a bioprocessing station (17) according to any one of claims 9 to 12 for performing a bioprocess.

20. Use according to claim 19, wherein the bioprocess component (18) comprises at least one tray (42) configured to dock onto the bioprocessing base (1) by interfacing with at least one interface element (4) of the bioprocessing base (1).

21. Use according to claim 19, wherein the bioprocess component (18) comprises a cartridge (20) configured to dock onto the bioprocessing base (1) by interfacing with the adapter module (9) of the bioprocessing base (1).

22. Method of processing a biological sample in a bioprocessing system (27) according to any one of claims 13 to 18, the method comprising: arranging at least two bioprocessing stations (17) in adjacent processing positions (34), generating a fluid-tight connection between the bioprocessing stations (17), preferably by welding, wherein preferably the reversible fluid-tight connection is established through one or more consumable elements which may comprise a tube set (24) and / or a bioprocess container (22), performing a bioprocess, and optionally severing the fluid-tight connection, preferably by sealing and cutting.

23. Method according to claim 22, wherein the performing of the bioprocess involves transferring fluid from one bioprocessing station (17) to another bioprocessing station (17).

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