Composition for promoting water supplementation, and preparation method therefor and use thereof

Abstract

A composition for promoting water supplementation, and a preparation method therefor and a use thereof. The composition at least comprises one or more of component I, component II, and component III, wherein component I is an oligosaccharide carbohydrate containing at least two glucose molecules linked together or a derivative thereof, component II is a chelate salt of an amino acid for supplying a mineral element and a derivative thereof, and component III is a common aid such as a sweetener or an acidulant or a conventional nutrient supplement such as vitamin B or vitamin K2 in food processing. The mass ratio of component I to component II to component III is (20-100):(0-50):(0-20). The composition can remarkably promote hydration, protect the body from dehydration, and further reduce fatigue during exercise, relieve exercise injuries, increase stamina, and promote body recovery.

Description

A composition for promoting hydration and its preparation method and application Technical Field

[0001] The present invention belongs to the technical field of sports nutrition food, and in particular relates to a composition for promoting hydration, and a preparation method and application thereof. Background Art

[0002] Water is a vital component of cells and body fluids, participating in metabolism, regulating body temperature, and lubricating joints and intestines. Losing even 10% of body water can severely disrupt physiological functions, while a loss of 20% can quickly lead to death. Besides water, human body water also contains common minerals such as calcium, magnesium, potassium, and sodium.

[0003] Intense exercise, prolonged exercise, and working in a continuously hot environment all lead to fluid loss, which is associated with increased sweat loss. Even a small water loss of around 2% of body weight can lead to impaired exercise tolerance and negatively impact cognitive ability and recovery. Excessive sweating leads to electrolyte loss and reduced body fluid volume, leading to physiological stress and a greater increase in the body's heat load. To offset the adverse effects of dehydration on cardiovascular function and exercise performance, it is now widely recommended to drink beverages rich in carbohydrates and electrolytes to provide the carbohydrates needed for energy, and to replenish fluids to alleviate dehydration and offset hyponatremia.

[0004] Furthermore, during exercise and work under dehydrated conditions, the contribution of anaerobic glucose metabolism and glycogen to energy production increases. Therefore, to maintain the ability to exercise and work for extended periods in hot ambient temperatures, hydration must consider not only the rate of fluid loss but also the increased utilization of carbohydrates. Hydration is related to the body's total water volume. This is an aspect of fluid regulation that involves the homeostatic regulation, composition, and distribution of fluid volume throughout the body. Adequate hydration prevents weakness and even reduces oxidative stress, which are hallmarks of high-intensity exercise, such as endurance sports. Furthermore, some studies have shown that drinking water can help alleviate frequent headaches.

[0005] Optimal hydration occurs when water or fluid intake is sufficient to replace water lost. If water intake is insufficient, dehydration can occur. Dehydration is dangerous and can lead to high blood sugar levels, cardiovascular disease, and urinary tract problems. Studies have shown that dehydration can even impair physical and cognitive performance and affect mood.

[0006] Given the importance of hydration status to physical performance and recovery, it seems crucial to examine the effects of different hydration strategies on the extent of muscle cell damage, exercise tolerance, and inflammatory status. The low-osmotic pressure hydration strategy of the present invention, comprising chelated salts of carbohydrates, amino acids, and their derivatives, can significantly promote hydration, protect the body from dehydration, and further reduce fatigue during exercise. Summary of the Invention

[0007] The present invention aims to overcome the problems existing in the prior art and provide a composition for promoting hydration and a preparation method and application thereof.

[0008] One of the objectives of the present invention is to provide a composition that promotes hydration. This composition can improve the body's bioimpedance, enhance hydration, and transport water across cell membranes, thereby promoting hydration. The composition is particularly suitable for restoring hydration and electrolyte balance after strenuous exercise, prolonged exercise, and work in high-temperature environments. Furthermore, the composition can replenish nutrients such as minerals, amino acids, and vitamins, alleviate sports injuries, increase endurance, and promote recovery.

[0009] The second object of the present invention is to provide a preparation method of the composition. Although the composition of the present invention can be prepared by a conventional solid particle preparation method, when the complex of amino acids and their amino acid hydrates or derivatives combined with mineral elements in the selected composition has an unpleasant taste, it can be treated by the application method of the flavor modifier combination mentioned in the application document No. 202310143495.2 submitted by the applicant Nanjing Shengde Chuangying Biotechnology Co., Ltd. on February 21, 2023, so as to avoid the use of sweeteners and flavors and make the product label more green and natural.

[0010] The third object of the present invention is to provide an application of the composition in regulating water absorption and electrolyte balance in the human body after strenuous exercise, long-term exercise and working in a high-temperature environment.

[0011] The purpose of the present invention and the solution to the technical problem are achieved by adopting the following technical solutions.

[0012] One aspect of the present invention provides a composition for promoting hydration, comprising at least one or more of component I, component II, and component III, wherein component I is an oligosaccharide carbohydrate or a derivative thereof bound by at least two glucose molecules, component II is a chelated salt of an amino acid and its derivative that provides mineral elements, and component III is an auxiliary material commonly used in food processing, such as a sweetener, an acidulant, or a nutrient supplement such as B vitamins or vitamin K2; the mass ratio of component I, component II, and component III is: (20-100): (0-50): (0-20).

[0013] In some preferred embodiments of the present invention, the component I is selected from one or more of the following groups, which consists of: maltotetraose, maltyl trehalose, trehalose, stachyose, raffinose, xylooligosaccharides, fructooligosaccharides, galacto-oligosaccharides, lactulose, isomaltooligosaccharides, lactofructooligosaccharides, chito-oligosaccharides, malto-oligosaccharides, isomerized lactose or its derivatives.

[0014] In a more preferred embodiment of the present invention, the component I is a mixture of maltotetraose and maltosyltrehalose.

[0015] Maltotetraose, a glucose tetramer linked by α-1,4-glycosidic bonds, is a novel maltooligosaccharide. It exhibits excellent salt and heat resistance, resists browning, and is well digested and absorbed by the human body, making it suitable for use as a dietary ingredient in children and athletes. Currently, commonly used sports drinks utilize monosaccharides or disaccharides such as glucose, sucrose, and maltose as carbohydrate energy supplements. However, these sugars have high osmotic pressures and can easily cause osmotic diarrhea in the user, so they are often supplemented with dextrin to adjust osmotic pressure. Research has found that maltotetraose, due to its minimal branching structure and linear oligosaccharide structure, has a low osmotic pressure and is efficiently decomposed and metabolized in the body. Therefore, it is the preferred carbohydrate and energy source in the present invention. Currently, no energy drinks or sports drinks using maltotetraose as a carbohydrate are available on the market.

[0016] Maltosyl trehalose is a carbohydrate composed of maltose and trehalose. It can serve as a nutrient source for probiotics, promoting their growth and reproduction, increasing their numbers, and inhibiting the growth of harmful bacteria, thereby maintaining the stability of the intestinal flora and improving intestinal health. It also has a regulatory effect on the immune system, protecting against the invasion of pathogenic microorganisms.

[0017] In some preferred embodiments of the present invention, the mineral elements of component II are selected from one or more of the following groups, which group consists of: sodium, potassium, calcium, magnesium, zinc, manganese, chromium, phosphorus, sulfur, iron chlorine, copper, iodine, molybdenum, cobalt, tin, vanadium, silicon, nickel, fluorine, selenium, strontium, lithium, iodine, and chlorine.

[0018] In some preferred embodiments of the present invention, the amino acids of component II are selected from one or more of the following groups: aspartic acid, threonine, serine, glutamic acid, glutamine, proline, glycine, alanine, cystine, cysteine, valine, methionine, leucine, isoleucine, tyrosine, phenylalanine, lysine, arginine, histidine, tryptophan, citrulline, ornithine, theanine, taurine, hydroxyproline, N-acetylneuraminic acid, hydroxyproline, γ-aminobutyric acid and its hydrates or derivatives thereof.

[0019] In some preferred embodiments of the present invention, the chelated salt of the amino acid derivative of component II is a complex of selected mineral elements and amino acids and their hydrates or derivatives, selected from one or more of the following groups, the group consisting of: magnesium L-aspartate, magnesium L-hydroxyproline, zinc theanine, zinc L-glycinate, calcium L-glutamate, sodium L-glycinate, manganese L-glycinate, magnesium L-glycinate, zinc methionine, copper lysine, iron L-hydroxyproline, iron lysine, methionine Manganese, zinc arginine, magnesium L-threonate, magnesium taurate, magnesium N-acetylneuraminic acid, magnesium hydroxyproline, magnesium gamma-aminobutyrate, calcium N-acetylneuraminic acid, calcium hydroxyproline, calcium gamma-aminobutyrate, manganese N-acetylneuraminic acid, manganese hydroxyproline, manganese gamma-aminobutyrate, zinc N-acetylneuraminic acid, zinc hydroxyproline, zinc gamma-aminobutyrate, potassium N-acetylneuraminic acid, potassium hydroxyproline, potassium gamma-aminobutyrate, sodium N-acetylneuraminic acid, sodium hydroxyproline, sodium gamma-aminobutyrate.

[0020] In some further preferred embodiments of the present invention, the amino acid chelate is sodium L-glutamate, zinc L-theanine, magnesium L-hydroxyproline, calcium L-aspartate, potassium L-glycinate, manganese L-glycinate, magnesium N-acetylneuraminic acid, magnesium hydroxyproline, magnesium γ-aminobutyrate, calcium N-acetylneuraminic acid, calcium hydroxyproline, calcium γ-aminobutyric acid, manganese N-acetylneuraminic acid, manganese hydroxyproline, manganese γ-aminobutyric acid, zinc N-acetylneuraminic acid, zinc hydroxyproline, zinc γ-aminobutyric acid, potassium N-acetylneuraminic acid, potassium hydroxyproline, potassium γ-aminobutyric acid, sodium N-acetylneuraminic acid, sodium hydroxyproline, and sodium γ-aminobutyric acid.

[0021] Amino acid chelates are complexes of mineral elements with amino acids, their hydrates, or their derivatives. Compared to simple amino acids or mineral elements, amino acid chelates have higher biological efficacy. Commonly used inorganic and organic salts, after ingestion, must form complexes with amino acids or other substances with the help of coenzymes before they can be absorbed. After absorption, the metal elements bind to certain proteins in the bloodstream and are transported to the required parts of the body to be effective. Amino acid complexes, on the other hand, are both the primary form of metal ion absorption and an intermediate in protein synthesis. Furthermore, their moderate stability constant allows them to be effectively released for utilization when needed. Therefore, amino acid chelates can simultaneously provide two essential nutrients—trace elements and amino acids—and thus possess dual nutritional benefits.

[0022] In some preferred embodiments of the present invention, the remaining components of the composition are auxiliary materials commonly used in food processing, such as sweeteners, acidulants, etc., or conventional nutrient supplements, such as B vitamins and vitamin K2. However, these components are not absolutely necessary and have no substantial impact on the composition of the present invention.

[0023] Short-term intense exercise, prolonged exercise, and physical activity performed in high ambient temperature and humidity can impair thermoregulatory processes, leading to severe dehydration and significant muscle stress due to endogenous and exogenous hyperthermia. Exercise- and environmental-induced muscle fiber damage triggers an inflammatory response associated with elevated blood levels of myoglobin (Mb), creatine kinase (CK), and lactate dehydrogenase (LDH). These proteins are widely used markers of skeletal muscle damage. Furthermore, post-exercise muscle damage triggers an inflammatory response associated with increased production of certain proinflammatory interleukins, such as IL-1β, which stimulates C-reactive protein (CRP) production in the liver. Interleukins are a large class of polypeptides known as cytokines, which are directly linked to inflammation associated with the infiltration of neutrophils and, later, macrophages into the injury site. Increased production of reactive oxygen species (ROS) due to intense exercise, dehydration, and hyperthermia further contributes to a pro-oxidant-antioxidant imbalance.

[0024] Dehydration reduces plasma volume, leading to decreased blood flow to contracting muscles, altered muscle cell metabolism, and impaired thermoregulation, particularly in hot environments. In endurance tests, fluid restriction during exercise has been shown to impede performance at high intensity. Rehydration has also been shown to improve subsequent exercise performance and is particularly important after prolonged exercise in hot and humid conditions.

[0025] Given the importance of hydration status to the body's ability to move and recover, appropriate hydration strategies and proper hydration are likely to effectively protect muscle cells from the effects of exogenous and endogenous exercise-induced heat stress, thereby avoiding exercise loss and reducing the sensitivity of cells to damage caused by oxidative stress due to dehydration. Conventional hydration generally uses isotonicity, mainly because it is equivalent to the osmotic pressure in the body. However, the inventors of this patent have discovered that a low-osmotic pressure hydration strategy using carbohydrates + amino acid chelates is more conducive to hydration and promotes water absorption by the human body.

[0026] In some preferred embodiments of the present invention, when the composition is formulated into a liquid, the mass fraction of component I in the solution ranges from 5% to 30%, preferably from 5% to 20%.

[0027] A second aspect of the present invention provides a method for preparing the aforementioned composition for promoting hydration, the method comprising the following steps:

[0028] a) Mixing: Weigh components I, II, and III according to the proportion and mix them evenly;

[0029] b) Granulation: A conventional wet granulation process is used: 5% to 10% by volume of a 70% to 90% ethanol solution is sprayed onto the mixed material, mixed evenly, and then dried at 40 to 60°C. The granules are sieved through a 40 to 80 mesh sieve, and the above wet granulation process is repeated on the obtained undersize material. Finally, all the materials are mixed to obtain the composition.

[0030] The preparation method of the composition for promoting hydration of the present invention is to weigh the components in the composition according to the said proportion, mix them evenly, and granulate them by wet or dry method to obtain the said composition. However, when the complex of the amino acid selected in component II and its hydrate or derivative with the selected mineral element has poor flavor (produces an unpleasant taste), it can be treated by the application method of the flavor improver combination mentioned in the application document with application number 202310143495.2 submitted by the applicant Nanjing Shengde Chuangying Biotechnology Co., Ltd. on February 21, 2023.

[0031] Conventional flavor-masking substances include sugars such as sucrose, high-intensity sweeteners, and flavors. Sugars like sucrose have a high osmotic pressure and can easily cause osmotic diarrhea in consumers, while high-intensity sweeteners often have a bitter taste and are not label-friendly. The application method for a flavor modifier combination, as described in application number 202310143495.2, filed on February 21, 2023 by applicant Nanjing Shengde Chuangying Biotechnology Co., Ltd., can effectively address these issues.

[0032] The third aspect of the present invention provides a product for regulating water absorption and electrolyte balance in the human body, comprising the aforementioned composition, or a composition obtained according to the aforementioned preparation method.

[0033] In some preferred embodiments of the present invention, the product may be in the form of powder, granules, liquid, or semi-solid, preferably in the form of liquid.

[0034] In some preferred embodiments of the present invention, the osmotic pressure of the product is between 10 mOsmol / L and 275 mOsmol / L, preferably between 50 mOsmol / L and 275 mOsmol / L.

[0035] In some preferred embodiments of the present invention, the sodium ion concentration in the product is between 10 mg / L and 150 mg / L, preferably between 10 mg / L and 100 mg / L.

[0036] A fourth aspect of the present invention provides a use of a composition for promoting hydration in the preparation of a product for regulating water absorption and electrolyte balance in the human body, wherein the composition is the composition described above, or a composition prepared according to the above-mentioned preparation method.

[0037] By means of the above technical solution, the present invention has at least the following advantages:

[0038] 1) The low-osmotic pressure hydrating composition of carbohydrates + amino acids and their derivative chelated salts of the present invention can improve the body's bioimpedance, enhance human hydration, transport water across cell membranes, and promote water replenishment in the body. It is particularly suitable for restoring body water and electrolyte balance after strenuous exercise, prolonged exercise, and working in high-temperature environments.

[0039] 2) The composition of the present invention can supplement nutrients such as minerals, amino acids, vitamins, etc., relieve sports injuries, increase endurance, and promote body recovery.

[0040] 3) The preparation method of the composition of the present invention can improve the unpleasant taste of some amino acid chelate salts. The finished product does not use flavors and high-intensity sweeteners, and has a green label.

[0041] The above description is only an overview of the technical solution of the present invention. In order to more clearly understand the technical means of the present invention and implement it according to the contents of the specification, the following is a detailed description of the preferred embodiments of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 shows a trend graph of cumulative urine volume after drinking different beverages;

[0043] FIG2 shows the BHI index of different beverages. DETAILED DESCRIPTION

[0044] In order to make the technical means, creative features, objectives and effects achieved by the present invention easier to understand, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts shall fall within the scope of protection of the present invention.

[0045] The present invention provides a composition for promoting hydration, which adopts a low osmotic pressure hydration strategy of carbohydrates + amino acid chelate salts, can improve the biological impedance of the human body, improve the hydration of the human body, transport water through the cell membrane, and promote the hydration of the human body. It is particularly suitable for the recovery of body water and electrolyte balance after strenuous exercise, long-term exercise and working in a high-temperature environment. At the same time, it can also supplement nutrients such as amino acids and vitamins, alleviate sports injuries, increase endurance, and promote body recovery. Another object of the present invention is to provide an application of the composition in the fields of strenuous exercise, long-term exercise and working in a high-temperature environment to solve the problem of efficient hydration. One object of the present invention is to provide a method for preparing a composition, which can improve the unpleasant taste of some amino acid chelate salts, does not require the use of flavors or high-intensity sweeteners to mask the taste, and has a green label.

[0046] Unless otherwise specified, the experimental methods used in the following examples are conventional methods.

[0047] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0048] The quantitative tests in the following examples were all repeated three times, and the average value was taken as the result.

[0049] Example 1: This example provides a composition for promoting hydration and a preparation method thereof, as follows:

[0050] The components are mixed in proportion by mass: 43.84% maltotetraose, 8.77% maltoyl trehalose, 0.05% sodium L-glutamate, 37.7% potassium L-aspartate, 7.01% magnesium N-acetylneuraminic acid, 0.35% sodium chloride, and 2.28% zinc N-acetylneuraminic acid.

[0051] Preparation method:

[0052] a) Mixing: After accurately weighing the above raw materials, sodium chloride, sodium L-glutamate, zinc N-acetylneuraminic acid, magnesium N-acetylneuraminic acid, maltoyl trehalose, potassium L-aspartate, and maltotetraose were added in sequence and mixed using a three-dimensional mixer for 1 hour;

[0053] b) Granulation: A conventional wet granulation process is employed: approximately 5% by volume of a 75% ethanol solution is sprayed onto the mixed materials. After uniform mixing, the mixture is dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The sieved portion is then subjected to the same wet granulation process. Finally, all materials are mixed to obtain the composition.

[0054] Example 2: This example provides a composition for promoting hydration and a preparation method thereof, as follows:

[0055] The components are mixed in proportion by mass: 40% maltotetraose, 12.61% maltoyl trehalose, 0.05% sodium L-glutamate, 37.7% potassium L-aspartate, 7.01% magnesium glycinate, 0.35% sodium chloride, and 2.28% zinc N-acetylneuraminic acid.

[0056] Preparation method:

[0057] a) Mixing: After accurately weighing the above raw materials, add sodium L-glutamate, sodium chloride, zinc N-acetylneuraminic acid, magnesium glycinate, maltoyl trehalose, potassium L-aspartate, and maltotetraose in sequence, and mix using a three-dimensional mixer for 1 hour;

[0058] b) Granulation: A conventional wet granulation process is employed: approximately 5% by volume of a 75% ethanol solution is sprayed onto the mixed materials. After uniform mixing, the mixture is dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The sieved portion is then subjected to the same wet granulation process. Finally, all materials are mixed to obtain the composition.

[0059] Example 3: This example provides a composition for promoting hydration and a preparation method thereof, as follows:

[0060] The components are mixed in proportion by mass: 35.52% maltotetraose, 17.76% maltoyl trehalose, 1.33% sodium L-glutamate, 37.65% potassium L-aspartate, 7.1% magnesium N-acetylneuraminic acid, 0.53% potassium chloride, and 0.11% calcium N-acetylneuraminic acid.

[0061] Preparation method:

[0062] a) Mixing: After accurately weighing the above raw materials, add N-acetylneuraminic acid calcium, potassium chloride, sodium L-glutamate, magnesium glycinate, magnesium N-acetylneuraminic acid, maltosyl trehalose, potassium L-aspartate, and maltotetraose in order, and mix using a three-dimensional mixer for 1 hour;

[0063] b) Granulation: A conventional wet granulation process is employed: approximately 5% by volume of an 80% ethanol solution is sprayed onto the mixed materials. After uniform mixing, the mixture is dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The sieved material is then subjected to the same wet granulation process. Finally, all materials are mixed to obtain the composition.

[0064] Example 4: This example provides a composition for promoting hydration and a preparation method thereof, as follows:

[0065] The components are proportioned according to mass percentage: maltotetraose 35.52%, maltoyl trehalose 17.76%, sodium L-glutamate 1.33%, potassium L-aspartate 37.65%, magnesium γ-aminobutyrate 7.1%, potassium N-acetylneuraminic acid 0.53%, and calcium L-aspartate 0.11%.

[0066] Preparation method:

[0067] a) Mixing: After accurately weighing the above raw materials, calcium L-aspartate, potassium chloride, sodium L-glutamate, magnesium γ-theanine, maltoyl trehalose, potassium L-aspartate, and maltotetraose were added in sequence and mixed using a three-dimensional mixer for 1 hour;

[0068] b) Granulation: A conventional wet granulation process is employed: approximately 5% by volume of a 75% ethanol solution is sprayed onto the mixed materials. After uniform mixing, the mixture is dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The sieved portion is then subjected to the same wet granulation process. Finally, all materials are mixed to obtain the composition.

[0069] Example 5: This example provides a composition for promoting hydration and a preparation method thereof, as follows:

[0070] The components are mixed in proportion by mass: 51.26% maltotetraose, 17.09% maltoyl trehalose, 0.47% sodium L-glutamate, 23.92% potassium theanine, 6.75% magnesium γ-aminobutyrate, and 0.51% potassium chloride.

[0071] Preparation method:

[0072] a) Mixing: After accurately weighing the above raw materials, add sodium L-glutamate, potassium chloride, γ-theanine magnesium, maltyl trehalose, potassium theaninate, and maltotetraose in sequence, and mix using a three-dimensional mixer for 1 hour;

[0073] b) Granulation: A conventional wet granulation process is employed: approximately 6% by volume of a 75% ethanol solution is sprayed onto the mixed materials. After uniform mixing, the mixture is dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The sieved material is then subjected to the same wet granulation process. Finally, all materials are mixed to obtain the composition.

[0074] Comparative Example 1: This comparative example provides a comparative example of the composition for promoting hydration according to Example 5, wherein the content of trace elements is the same, but the source of the trace elements is not amino acids, as follows:

[0075] The components are mixed in proportion by mass: 33.75% maltotetraose, 11.25% maltoyl trehalose, 10.57% magnesium citrate, 44.15% potassium gluconate, and 0.28% sodium chloride.

[0076] Preparation method: After accurately weighing the above raw materials, the same preparation method as in Example 5 was used to sequentially mix sodium chloride, magnesium citrate, maltoyl trehalose, maltotetraose, and potassium gluconate. After uniform mixing, the mixture was sprayed with a 75% ethanol solution (6% by volume of the total mass) to form granules. The granules were dried in a 60°C thermostat, sized, and passed through a 40-mesh sieve. The wet granulation process was repeated for the sieved material. Finally, all materials were mixed to obtain the composite granules.

[0077] Comparative Example 2: This comparative example provides a composition prepared according to Comparative Example 1.

[0078] Test Example 1: Effect of Drinks Prepared from the Composition on Hydration

[0079] Drinks with different ingredients were prepared according to the recipes in Table 1.

[0080] Table 1 Beverage formula and content

[0081]

[0082] The preparation method of each of the above beverages is as follows:

[0083] According to the order of numbers in Table 1, 1000 mL of pure water was measured respectively, heated to boiling, maintained for 3 minutes, and the corresponding mass percentage of the hydrating composition was slowly added in sequence, mixed evenly, cooled, and packaged for storage to obtain beverages numbered 1 to 7.

[0084] Seventy-two volunteers were recruited and randomly divided into nine groups. Inclusion criteria were as follows: participants aged 18 to 40 years and generally healthy. Participants were ineligible for inclusion if they had any of the following: a history of cardiovascular, renal, musculoskeletal, or metabolic disease; a history of urinary incontinence or frequent urination; a pacemaker or other electrical implant; a history of hand or foot amputation; or current pregnancy. All participants abstained from food, alcohol, nicotine, or medication for at least 8 hours prior to testing, and from alcohol, exercise, or strenuous physical activity for at least 24 hours. Before testing, participants were instructed to drink 500 mL of purified water within 15 minutes. Upon arrival at the laboratory, participants sat quietly in a comfortable environment for 10 minutes. Participants were then instructed to empty their bladders. Participants ingested a 1 liter test sample over 30 minutes (an average of 250 mL every 7.5 minutes). Urine volume was then collected over the next four hours and weighed after the test. All sample osmolality was measured using a BS-100 freezing point osmometer (Table 2). To assess the significance of the observed differences in BHI between the blank and each test sample, the differences were compared to the normal variation determined by a separate repeatability analysis. To achieve this, participants consumed the same beverage on two occasions for this repeatability analysis. The test samples were pure water, Gatorade, and drinks 1 through 7.

[0085] Table 2 Osmotic pressure of test samples

[0086]

[0087] The beverage hydration index (BHI) was calculated as the mass of urine excreted within two hours after ingesting one liter of pure water (i.e., control condition) divided by the mass of urine excreted within two hours after ingesting the formula beverage.

[0088] Figure 1 shows a trend chart of cumulative urine volume after ingestion of the test samples. Examples 1-5 and Comparative Examples 1-2 in the figure refer to beverages 1-7 prepared from the corresponding examples and comparative examples. As can be seen from Figure 1, there was no significant difference in urine quality in the tests conducted immediately after ingestion of the test samples. Compared to purified water, the effect size for cumulative urine volume over 4 hours for beverages prepared from the compositions of Examples 1-5 was significantly lower than that for beverages prepared from the compositions of Comparative Examples 1-2 and the positive control, Gatorade.

[0089] Figure 2 is a BHI trend chart, where Examples 1-5 and Comparative Examples 1-2 refer to beverages 1-7 prepared from the corresponding Examples and Comparative Examples. As can be seen from the chart, compared to pure water, the BHIs of the beverages prepared from the compositions of Examples 1-5 were significantly higher than those of the beverages prepared from the compositions of Comparative Examples 1-2 and the positive control, Gatorade.

[0090] Combined with the osmotic pressure data in Table 2, it can be seen that the cumulative urine volume and hydration index of the beverages prepared from the compositions of Examples 1 to 5 are better than those of isotonic beverages and hypertonic beverages. The hydration index indirectly indicates that the beverages have a better water retention effect in the body and a better hydration effect.

[0091] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred embodiment as above, it is not intended to limit the present invention. Any technician familiar with this profession can make slight changes or modifications to equivalent embodiments of the methods and technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A composition for promoting hydration, characterized in that: The composition comprises at least one or more of component I, component II, and component III, wherein component I is an oligosaccharide carbohydrate or a derivative thereof bound by at least two glucose molecules, component II is a chelated salt of an amino acid and its derivative that provides mineral elements, and component III is an auxiliary material commonly used in food processing, such as a sweetener, an acidulant, or a nutrient supplement such as B vitamins and vitamin K2; the mass ratio of component I, component II, and component III is: (20-100): (0-50): (0-20).

2. A composition for promoting hydration according to claim 1, characterized in that The component I is selected from one or more of the following groups, which consists of: maltotetraose, maltosyl trehalose, trehalose, stachyose, raffinose, xylooligosaccharides, fructooligosaccharides, galacto-oligosaccharides, lactulose, isomaltooligosaccharides, lactofructooligosaccharides, chito-oligosaccharides, malto-oligosaccharides, isomerized lactose or its derivatives.

3. A composition for promoting hydration according to claim 1, characterized in that: The mineral elements of component II are selected from one or more of the following groups: sodium, potassium, calcium, magnesium, zinc, manganese, chromium, phosphorus, sulfur, chlorine, iron, copper, iodine, molybdenum, cobalt, tin, vanadium, silicon, nickel, fluorine, selenium, strontium, lithium, iodine, and chlorine.

4. A composition for promoting hydration according to claim 1, characterized in that: The amino acids of component II are selected from one or more of the following groups: aspartic acid, threonine, serine, glutamic acid, glutamine, proline, glycine, alanine, cystine, cysteine, valine, methionine, leucine, isoleucine, tyrosine, phenylalanine, lysine, arginine, histidine, tryptophan, citrulline, ornithine, theanine, taurine, hydroxyproline, N-acetylneuraminic acid, hydroxyproline, γ-aminobutyric acid and their hydrates or derivatives thereof.

5. A composition for promoting hydration according to claim 1, characterized in that: The chelated salt of the amino acid derivative of component II is a complex of selected mineral elements and amino acids, hydrates thereof or derivatives thereof, and is selected from one or more of the following groups, which consists of: magnesium L-aspartate, magnesium L-hydroxyproline, zinc theanine, zinc L-glycinate, calcium L-glutamate, sodium L-glycinate, manganese L-glycinate, magnesium L-glycinate, zinc methionine, copper lysinate, iron L-hydroxyproline, iron lysinate, manganese methionine, zinc arginine, magnesium L-threonate, magnesium taurine, magnesium N-acetylneuraminic acid, magnesium hydroxyproline, magnesium γ-aminobutyrate, calcium N-acetylneuraminic acid, calcium hydroxyproline, calcium γ-aminobutyric acid, manganese N-acetylneuraminic acid, manganese hydroxyproline, manganese γ-aminobutyric acid, zinc N-acetylneuraminic acid, zinc hydroxyproline, zinc γ-aminobutyric acid, potassium N-acetylneuraminic acid, potassium hydroxyproline, potassium γ-aminobutyric acid, sodium N-acetylneuraminic acid, sodium hydroxyproline, and sodium γ-aminobutyric acid.

6. A composition for promoting hydration according to claim 1, characterized in that: When the composition is formulated into a liquid, the proportion of component I in the liquid ranges from 5% to 20%.

7. A method for preparing the composition for promoting hydration according to any one of claims 1 to 6, characterized in that: The method comprises the following steps: a) Mixing: Weigh components I, II, and III according to the proportion and mix them evenly; b) Granulation: A conventional wet granulation process is used: 5% to 10% by volume of a 70% to 90% ethanol solution is sprayed onto the mixed material, mixed evenly, and then dried at 40 to 60°C. The granules are sieved through a 40 to 80 mesh sieve, and the above wet granulation process is repeated on the obtained undersize material. Finally, all the materials are mixed to obtain the composition.

8. A product for regulating water absorption and electrolyte balance in the human body, characterized in that: A composition comprising the composition according to any one of claims 1 to 6, or a composition prepared according to the preparation method according to claim 7.

9. A product for regulating water absorption and electrolyte balance in the human body according to claim 8, characterized in that: The product is in liquid form, and the osmotic pressure of the product is between 50mOsmol / L and 275mOsmol / L; the sodium ion concentration in the product is between 10mg / L and 100mg / L.

10. Use of a composition for promoting hydration in the preparation of a product for regulating water absorption and electrolyte balance in the human body, characterized in that: The composition is the composition according to any one of claims 1 to 6, or the composition prepared by the preparation method according to claim 7.

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