Hair growth promoter

Abstract

The purpose of the present invention is to provide a novel hair growth promoter. The features of the present invention for solving the problem are as follows. 1. A hair growth promoter containing an extract of yellow strawberry guava fruit as an active ingredient. 2. An agent for promoting vascular endothelial cell growth factor production, containing an extract of yellow strawberry guava fruit as an active ingredient. 3. A food or drink composition for promoting hair growth, containing the hair growth promoter as an active ingredient. 4. A pharmaceutical composition for promoting hair growth, containing the hair growth promoter as an active ingredient. 5. A hair cosmetic composition for promoting hair growth, containing the hair growth promoter as an active ingredient. 6. A topical skin preparation composition for promoting hair growth, containing the hair growth promoter as an active ingredient. 7. A hair growth promoter for use in oral administration, containing an aqueous ethanol extract of yellow strawberry guava fruit as an active ingredient.

Description

Hair growth promoter

[0001] The present invention relates to a novel hair growth promoter.

[0002] Hair grows and falls out repeatedly according to a cyclical hair cycle (hair cycle) consisting of an anagen phase, a regressive phase, and a telogen phase. The stage of this hair cycle, from the telogen phase to the anagen phase, in which new hair follicles are formed, is considered to be the most important for hair growth, and dermal papilla cells are thought to play an important role in the proliferation and differentiation of hair follicle epithelial cells during this stage. Dermal papilla cells are located inside hair follicle epithelial cells, which are composed of outer root sheath cells and matrix cells near the hair root, in the basement membrane-enclosed part of the hair root. They act on hair follicle epithelial cells to promote their proliferation, and thus play an important role in the proliferation and differentiation of hair follicle epithelial cells and hair formation (see Non-Patent Document 1).

[0003] As such, dermal papilla cells play the most important role in the proliferation and differentiation of hair follicle epithelial cells and in hair formation, and a method has been proposed in the past in which a target substance is brought into contact with cultured dermal papilla cells, and the presence or absence and / or strength of the proliferation activity of the cells is identified to assay the hair growth-promoting effect of the target substance (see Patent Document 1).

[0004] In addition, as herbal medicines having the effect of promoting the proliferation of hair papilla cells, for example, Astragalus Root extract, Coptis Rhizome extract, and Anemone Cylindrica extract have been proposed (see Patent Documents 2 and 3). As such, there is a strong demand from consumers for various natural preparations that are safe and productive, can be taken daily, are inexpensive, and have excellent hair papilla cell proliferation promoting effects, but at present, no fully satisfactory products have been provided. Furthermore, while hair growth promoters have traditionally been used as hair tonics, etc., no sufficiently effective hair growth promoters are known for oral administration.

[0005] On the other hand, vascular endothelial growth factor (VEGF) is a glycoprotein with a molecular weight of 34-46 kDa. It was discovered in the culture medium of pituitary follicular cells as a growth factor specific to vascular endothelial cells and was found to be identical to vascular permeability factor (VPF). VEGF is known to be produced not only by pituitary cells but also by normal cells such as smooth muscle cells, macrophages, alveolar epithelial cells, hepatocytes, and dermal papilla cells. VEGF acts on vascular endothelial cells, promoting cell proliferation and migration and angiogenesis. VEGF is known to be strongly expressed during the development of the fetal heart. It has been reported that VEGF gene deficiency causes vascular abnormalities and fetal death, suggesting that VEGF plays an extremely important role in individual development and tissue formation. Recently, it has been reported that VEGF-C, a new member of the VEGF family, mediates lymphatic vessel growth in the skin as a potent lymphangiogenic factor.

[0006] Incidentally, it is known that outer root sheath cells and dermal papilla cells in hair follicles produce VEGF (see, for example, Non-Patent Documents 2 and 3). It has been found that inhibiting VEGF production in hair follicles leads to a delay in the anagen phase of the hair cycle and a decrease in hair follicle size (see, for example, Non-Patent Document 4), demonstrating the importance of VEGF in the development and regeneration of hair follicles. "Trends Genet," 1992, Vol. 8, pp. 56-61; J. Invest. Dermatol., 106, 17-23 (1996); Arch. Dermatol. Res., 209, 661-668 (1998); J. Clin. Invest. , 107, 409-417 (2001) JP-A-10-229978, JP-A-9-208431, JP-A-11-12134

[0007] Under these circumstances, the present inventors orally administered an extract of yellow strawberry guava (hereinafter sometimes referred to as "strawberry guava") to mice and found that the extract promoted the production of vascular endothelial growth factor (VEGF) and also had a hair growth promoting effect, leading to the completion of the present invention. That is, an object of the present invention is to provide a novel hair growth promoting agent.

[0008] The features of the present invention for solving the above problems are as follows: 1. A hair growth stimulant containing an extract of yellow strawberry guava fruit as an active ingredient. 2. A vascular endothelial cell growth factor production promoter containing an extract of yellow strawberry guava fruit as an active ingredient. 3. A food or drink composition for promoting hair growth containing the hair growth stimulant as an active ingredient. 4. A pharmaceutical composition for promoting hair growth containing the hair growth stimulant as an active ingredient. 5. A hair cosmetic composition for promoting hair growth containing the hair growth stimulant as an active ingredient. 6. A topical skin composition for promoting hair growth containing the hair growth stimulant as an active ingredient. 7. A hair growth stimulant for oral administration containing a hydroethanolic extract of yellow strawberry guava fruit as an active ingredient. 8. A method for promoting hair growth in humans, comprising: a. obtaining a polar solvent extract from a raw material of yellow strawberry guava fruit; and b. administering an effective amount of the polar solvent extract to humans orally or parenterally to promote the production of vascular endothelial cell growth factor in the human.

[0009] FIG. 1 is a schematic diagram showing a method for evaluating the hair growth effect of strawberry guava in C3H mice. FIG. 2 is a photograph of the back in an evaluation of hair growth effect of strawberry guava in C3H mice. FIG. 3 is a graph showing the evaluation of hair growth effect of strawberry guava in C3H mice. FIG. 4 is a graph showing the evaluation results of the vascular endothelial growth factor production effect of strawberry guava in C3H mice. FIG. 5 is a schematic diagram showing a method for evaluating the blood flow-increasing effect of strawberry guava in C3H mice. FIG. 6 is a graph showing the evaluation results of the blood flow-increasing effect of strawberry guava in C3H mice.

[0010] The present invention is characterized in that an extract of yellow strawberry guava fruit is used as an active ingredient.

[0011] It is preferable to use a polar solvent as the extraction solvent. Examples of polar solvents that can be used include water, methanol, ethanol, isopropyl alcohol, 1,3-butylene glycol, ethylene glycol, propylene glycol, glycerin, and ethyl acetate. Two or more of these solvents may be mixed. Preferably, water or aqueous ethanol, which is a mixture of water and ethanol, is used as the extraction solvent, as this allows for efficient extraction of active ingredients.

[0012] When water is used as the extraction solvent, the type of water is not particularly limited, and tap water, distilled water, mineral water, alkaline ionized water, deep sea water, etc. can be used.

[0013] When using aqueous ethanol as the extraction solvent, the ethanol concentration is not particularly limited, but it is particularly preferable that the ethanol concentration be 10 to 99% (wt / wt), and preferably 20 to 80% (wt / wt). The reason for setting the ethanol concentration at 99% (wt / wt) or less is that if the ethanol concentration is too high, oil will easily dissolve in the aqueous ethanol.

[0014] The extraction temperature should be 20 to 80° C., preferably about 40 to 50° C. If the extraction temperature is too low, the active ingredient is difficult to extract, and if the extraction temperature is too high, the active ingredient decomposes, resulting in a decrease in physiological activity (hair growth promoting effect).

[0015] As the extraction method, any method such as stirring extraction, continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction can be employed, and any device can be used at room temperature or under reflux heating.

[0016] Specifically, the extraction method involves placing the raw strawberry guava fruit in a processing tank filled with an extraction solvent and stirring to extract the active ingredients. For example, when using aqueous ethanol as the extraction solvent, the extraction solvent is used in an amount 2 to 100 times (by weight) the amount of the raw material, and extraction is carried out for 30 minutes to 2 hours. After the active ingredients have been extracted into the solvent, the extract is obtained by filtering to remove the extraction residue.

[0017] Thereafter, the extract is subjected to treatments such as dilution, concentration, purification, and drying according to conventional methods to obtain the agent according to the present invention.

[0018] Specifically, the extraction method involves placing the raw material (yellow strawberry guava fruit) in a processing tank filled with an extraction solvent and stirring to extract the active ingredients. For example, when using water or aqueous ethanol as the extraction solvent, the extraction solvent is used in an amount 2 to 100 times (by weight) the amount of the raw material, and extraction is carried out for 30 minutes to 2 hours. After the active ingredients have been extracted into the solvent, the extract is obtained by filtering to remove the extraction residue.

[0019] The extract is then diluted, concentrated, purified, dried, and other processes according to conventional methods to produce the hair growth promoter of the present invention. The purification method is preferably such that the extract is passed through a synthetic adsorption resin, gel filtration resin, or the like to adsorb the active ingredient, which is then eluted with methanol, ethanol, or the like and concentrated.

[0020] The hair growth stimulant of the present invention can be used as an ingredient in various foods and beverages. Examples of foods and beverages include general foods such as confectioneries (gum, candy, caramel, chocolate, cookies, snacks, jelly, gummy candy, tablet candy, etc.), noodles (soba, udon, ramen, etc.), dairy products (milk, ice cream, yogurt, etc.), seasonings (miso, soy sauce, etc.), soups, beverages (juice, coffee, black tea, green tea, carbonated drinks, sports drinks, etc.), health foods (tablets, capsules, etc.), and nutritional supplements (nutritional drinks, etc.). The agent of the present invention can be appropriately incorporated into these foods and beverages.

[0021] These foods and beverages can contain various ingredients depending on the type, and the following food ingredients can be used: glucose, fructose, sucrose, maltose, sorbitol, stevioside, corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerin fatty acid esters, polyglycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, propylene glycol fatty acid esters, gum arabic, carrageenan, casein, gelatin, pectin, agar, B vitamins, nicotinamide, calcium pantothenate, amino acids, calcium salts, colorings, flavorings, and preservatives.

[0022] As a specific manufacturing method, the agent of the present invention is spray-dried or freeze-dried together with powdered cellulose, and then formed into a powder, granules, tablets, or solution, which can be easily incorporated into foods and beverages (instant foods, etc.). The agent of the present invention can also be dissolved in, for example, fats and oils, ethanol, glycerin, or a mixture thereof to form a liquid, which can then be added to beverages or solid foods. If necessary, it can also be mixed with a binder such as gum arabic or dextrin to form a powder or granules, which can then be added to beverages or solid foods.

[0023] When the agent of the present invention is applied to food or drink, the amount of the active ingredient added is preferably 1 to 20 wt % in total relative to the food or drink.

[0024] The hair growth stimulant of the present invention may be used as a raw material for pharmaceuticals (medicines: including pharmaceuticals and quasi-drugs). Pharmaceutical preparations can be produced by appropriately blending the agent of the present invention with raw materials for pharmaceutical preparations. Examples of pharmaceutical raw materials that can be incorporated into the agent of the present invention include excipients (glucose, lactose, sucrose, sodium chloride, starch, calcium carbonate, kaolin, crystalline cellulose, cocoa butter, hardened vegetable oil, kaolin, talc, etc.), binders (distilled water, saline, ethanol water, simple syrup, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, potassium phosphate, polyvinylpyrrolidone, etc.), disintegrants (sodium alginate, agar, sodium bicarbonate, calcium carbonate, sodium lauryl sulfate, stearate monoglyceride, starch, lactose, powdered gum arabic, gelatin, ethanol, etc.), disintegration inhibitors (sucrose, stearin, cocoa butter, hydrogenated oil, etc.), absorption enhancers (quaternary ammonium base, sodium lauryl sulfate, etc.), adsorbents (glycerin, starch, lactose, kaolin, bentonite, silicic acid, etc.), and lubricants (purified talc, stearates, polyethylene glycol, etc.).

[0025] The hair growth stimulant of the present invention can generally be administered orally in the form of tablets, pills, soft or hard capsules, fine granules, powders, granules, liquids, etc., but may also be administered parenterally. When administered parenterally, it can be administered in the form of a solution or with the addition of a dispersant, suspending agent, stabilizer, etc., by local tissue administration, intradermal, subcutaneous, intramuscular, or intravenous injection. It may also be in the form of a suppository, etc. Furthermore, it can be administered as an eye drop.

[0026] The dosage may vary depending on factors such as the administration method, the condition, and the patient's age. For adults, the dosage is typically 0.5 to 5,000 mg of active ingredient per day, and for children, it is typically around 0.5 to 3,000 mg. The blending ratio of the hair growth promoter can be varied as needed depending on the dosage form, but is typically around 0.3 to 15.0 wt% for oral or mucosal administration and around 0.01 to 10 wt% for parenteral administration. The dosage varies depending on various conditions, so a smaller amount than the above may be sufficient, or it may be necessary to administer a larger amount than the above range.

[0027] The hair growth stimulant of the present invention can be used primarily as a hair cosmetic, which is not particularly limited and can be used, for example, as a hair tonic, shampoo, rinse, treatment, hair gel, hair cream, hair treatment lotion, hair foam, etc.

[0028] When the hair cosmetic composition is used as a hair tonic, it can be used in combination with other hair tonic ingredients along with the hair growth promoter of the present invention. In this case, the hair tonic ingredients to be blended are not particularly limited, and examples thereof include estrone, estradiol, ethylestradiol, vitamin E and derivatives, Swertia japonica extract, garlic extract, carrot extract, aloe extract, Enmeiso (Citrus Root), Gentian extract, Angelica Root extract, cepharanthine, Cordyceps sinensis extract, carpronium chloride, minoxidil, diazoxide, capsicum tincture, nonylic acid vanillamide, Cantharides tincture, Ginger tincture, peppermint oil, 1-menthol, camphor, nicotinic acid and derivatives, lactic acid and derivatives, resorcinol, salicylic acid, hexachlorophene, trichlorocarbanilide, bithionol, undecyl Further compounds that may be added include oleic acid, zinc and its derivatives, pyridoxine and its derivatives, sulfur, quaternary ammonium salts such as benzalcoronium chloride, alkyldiaminoethylglycine chloride solution, isopropylethylphenol, triclosan, Sophora root, photosensitizer No. 301, hinokitiol, pantothenyl ethyl ether, placenta extract, biotin, pentadecanoic acid glyceride, pantothenic acid and derivatives, glycyrrhizic acid, glycyrrhetinic acid, oxendolone, arachidonic acid, diphenhydramine hydrochloride, Lithospermum root extract, Angelica Root extract, Cistanche Tubulosa extract, echinacoside, acteoside, vitamins, amino acids, etc.

[0029] When the above-mentioned hair cosmetic composition is used as a shampoo, the hair growth promoter of the present invention can be blended with a cleanser, a conditioning agent, etc.

[0030] The following components can be used as the cleaning agent. Anionic surfactants: fatty acid soap, α-acylsulfonate, alkylsulfonate, alkylarylsulfonate, alkylnaphthalenesulfonate, alkyl sulfate, POE alkyl ether sulfate, alkylamide sulfate, alkyl phosphate, POE alkyl phosphate, alkylamide phosphate, alkyloylalkyl taurine salt, N-acylamino acid salt, POE alkyl ether carboxylate, alkyl sulfosuccinate, sodium alkylsulfoacetate, acylated hydrolyzed collagen peptide salt, perfluoroalkyl phosphate ester, etc. Amphoteric surfactants: carboxybetaine type, amidobetaine type, sulfobetaine type, hydroxysulfobetaine type, amidosulfobetaine type, phosphobetaine type, aminocarboxylate type, imitazoline derivative type, amidoamine type, etc. Nonionic surfactants: propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE-POP copolymers, POE-POP alkyl ethers, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, hydrogenated soybean phospholipid, and the like.

[0031] The following ingredients can be used as conditioning agents: Cationic surfactants: alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, etc.

[0032] When the hair growth promoter of the present invention is used as the above-mentioned hair cosmetic composition as a hair rinse or hair treatment, it is generally preferable to use an oil, a conditioning agent, an emulsifier, etc., and further to blend a thickener, a stabilizer, a moisturizer, a preservative, etc.

[0033] In this case, as the oil agent, higher alcohols, higher fatty acids, ester oils, hydrocarbon oils, natural oils, silicone oils, etc. can be used. Specifically, the following can be used. (1) Examples of higher alcohols: Lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, octyldodecanol, etc. (2) Examples of higher fatty acids: Caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid, arachidonic acid, behenic acid, erucic acid, 2-ethylhexanoic acid, etc. (3) Examples of ester oils: Glyceryl Tri-2-Ethylhexanoate, Cetyl 2-Ethylhexanoate, Isopropyl Myristate, Butyl Myristate, Isopropyl Palmitate, Ethyl Stearate, Octyl Palmitate, Isocetyl Isostearate, Butyl Stearate, Butyl Myristate, Ethyl Linoleate, Isopropyl Linoleate, Ethyl Oleate, Isocetyl Myristate, Isostearyl Myristate, Isostearyl Palmitate, Octyldodecyl Myristate, Isocetyl Isostearate, Diethyl Sebacate, Diisopropyl Adipate, Isoarachyl Neopentanoate, Caprylic / Capric Triglyceride, Trimethylolpropane Tri-2-Ethylhexanoate, Triisostearate Methylolpropane, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isocetyl myristate, isostearyl myristate, isocetyl palmitate, isostearyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isopropyl isostearate, cetostearyl 2-ethylhexanoate, stearyl 2-ethylhexanoate,Hexyl isostearate, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprate, propylene glycol dicaprylate, propylene glycol dicaprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate, polyglycerin isostearate, dipropyl carbonate, dialkyl carbonate (C12-18), triisostearate lactyl, triisoarachyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, and isostearyl 12-stearoylhydroxystearate. (4) Examples of hydrocarbon oils include squalane, squalene, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. (5) Examples of natural oils include animal oils and their hardened oils such as beef tallow, hardened beef tallow, lard, hardened lard, horse oil, hardened horse oil, mink oil, orange roughy oil, fish oil, hardened fish oil, egg yolk oil, avocado oil, almond oil, olive oil, cacao butter, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil,Examples of suitable oils include vegetable oils and their hydrogenated oils such as rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, medlar oil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, and hydrogenated coconut oil; beeswax, high acid value beeswax; lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin; and waxes such as carnauba wax and montan wax. (6) Examples of silicone oils include dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, and silicone RTV rubber.

[0034] The conditioning agent may be a cationic surfactant, silicone oil, or the like. The cationic surfactant may be the same as that used in the shampoo. Furthermore, the silicone oil may be the same as that used in the oil agent.

[0035] The emulsifier may be a cationic surfactant, a nonionic surfactant, or the like. The cationic surfactant may be the same as that used in the shampoo described above. The nonionic surfactant may be PEG-80 hydrogenated castor oil, POE alkyl ethers such as POE cetyl ether, POE propylene glycol, sorbitan monostearate, or the like.

[0036] The thickener may be, for example, hydroxyethyl cellulose, but is not limited to this.

[0037] As the stabilizer, for example, glycerin, propylene glycol, butylene glycol, ethanol, etc. can be used.

[0038] The hair growth promoter of the present invention can also be expected to have a hair growth promoting effect when used as a topical skin preparation (including cosmetics, pharmaceuticals, and quasi-drugs). Examples of the form of a topical skin preparation that can incorporate the hair growth promoter of the present invention include emulsions, soaps, facial cleansers, bath additives, creams, emulsions, skin lotions, colognes, shaving creams, shaving lotions, cosmetic oils, suntan / sunscreen lotions, face powders, foundations, perfumes, face masks, nail creams, enamel, enamel removers, eyebrow pencils, blushers, eye creams, eye shadows, mascaras, eyeliners, lipsticks, lip balms, shampoos, rinses, hair dyes, dispersions, and cleansers. Examples of the form of pharmaceuticals or quasi-drugs that can incorporate the hair growth promoter of the present invention include ointments, creams, topical liquids, and the like.

[0039] In addition to the hair growth promoter of the present invention, the above-mentioned external skin preparations can contain, to the extent that the hair growth promoting effect is not impaired, ingredients that are incorporated into external skin preparations such as cosmetics and quasi-drugs, oils, higher alcohols, fatty acids, ultraviolet absorbers, powders, pigments, surfactants, polyhydric alcohols / sugars, polymers, physiologically active ingredients, solvents, antioxidants, fragrances, preservatives, etc. Examples are listed below, but the present invention is not limited to these examples.

[0040] (1) Examples of oils Ester-based oil phase components: glyceryl tri-2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, butyl stearate, butyl myristate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipate, isoarachyl neopentanoate, caprylic / capric triglyceride, trimethylolpropane tri-2-ethylhexanoate, trimethylolpropane triisostearate, pentaerythritol tetra-2-ethylhexanoate, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, cetyl ricinoleate, isostearyl laurate, isotridecyl myristate, isomyristate Isocetyl, Isostearyl Myristate, Isocetyl Palmitate, Isostearyl Palmitate, Octyl Stearate, Isocetyl Stearate, Isodecyl Oleate, Octyldodecyl Oleate, Octyldodecyl Linoleate, Isopropyl Isostearate, Cetostearyl 2-Ethylhexanoate, Stearyl 2-Ethylhexanoate, Hexyl Isostearate, Ethylene Glycol Dioctanoate, Ethylene Glycol Dioleate, Propylene Glycol Dicaprate, Propylene Glycol Dicaprylate, Di Propylene glycol caprylate, neopentyl glycol dicaprate, neopentyl glycol dioctanoate, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyldecyl neodecanoate, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerin oleate,Polyglycerin isostearate, dipropyl carbonate, dialkyl carbonate (C12-18), triisocetyl citrate, triisoarachidyl citrate, triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, 2-ethylhexyl hydroxystearate, di-2-ethylhexyl succinate, diisoadipate Isobutyl, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, cholesteryl isostearate, cholesteryl hydroxystearate, cholesteryl oleate, dihydrocholesteryl oleate, phytosteryl isostearate, phytosteryl oleate, isocetyl 12-stearoylhydroxystearate, stearyl 12-stearoylhydroxystearate, isostearyl 12-stearoylhydroxystearate, etc. Hydrocarbon oil phase components include squalane, liquid paraffin, α-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybutene, microcrystalline wax, petrolatum, etc. Animal and vegetable oils and their hydrogenated oils, and naturally derived waxes: beef tallow, hydrogenated beef tallow, lard, hydrogenated lard, horse oil, hydrogenated horse oil, mink oil, orange roughy oil, fish oil, hydrogenated fish oil, egg yolk oil and other animal oils and their hydrogenated oils, avocado oil, almond oil, olive oil, cocoa butter, kiwi seed oil, apricot kernel oil, kukui nut oil, sesame oil, wheat germ oil, rice germ oil, rice bran oil, safflower oil, shea butter, soybean oil, evening primrose oil, perilla oil, tea seed oil, camellia oil, corn oil, rapeseed oil, Examples of suitable oils include vegetable oils and their hydrogenated oils, such as hydrogenated rapeseed oil, palm kernel oil, hydrogenated palm kernel oil, palm oil, hydrogenated palm oil, peanut oil, hydrogenated peanut oil, castor oil, hydrogenated castor oil, sunflower oil, grape seed oil, jojoba oil, hydrogenated jojoba oil, macadamia nut oil, medlar oil, cottonseed oil, hydrogenated cottonseed oil, coconut oil, and hydrogenated coconut oil, as well as waxes such as beeswax, high acid value beeswax, lanolin, reduced lanolin, hydrogenated lanolin, liquid lanolin, carnauba wax, and montan wax. Silicone-based oil phase components: dimethylpolysiloxane, methylphenylpolysiloxane, methylcyclopolysiloxane,Examples of suitable oil phase components include octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, methylhydrogenpolysiloxane, polyether-modified organopolysiloxane, dimethylsiloxane-methylcetyloxysiloxane copolymer, dimethylsiloxane-methylstearoxysiloxane copolymer, alkyl-modified organopolysiloxane, terminal-modified organopolysiloxane, amino-modified silicone oil, amino-modified organopolysiloxane, dimethiconol, silicone gel, acrylic silicone, trimethylsiloxysilicate, and silicone RTV rubber. Fluorine-based oil phase components include perfluoropolyether, fluorine-modified organopolysiloxane, fluorinated pitch, fluorocarbon, fluoroalcohol, and fluoroalkyl-polyoxyalkylene-co-modified organopolysiloxane.

[0041] (2) Examples of higher alcohols include lauryl alcohol, myristyl alcohol, cetyl alcohol, stearyl alcohol, isostearyl alcohol, oleyl alcohol, behenyl alcohol, 2-ethylhexanol, hexadecyl alcohol, and octyldodecanol.

[0042] (3) Examples of fatty acids include caprylic acid, capric acid, undecylenic acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, isostearic acid, oleic acid, linoleic acid, linolenic acid, arachidonic acid, behenic acid, erucic acid, and 2-ethylhexanoic acid.

[0043] (4) Examples of ultraviolet absorbers: para-aminobenzoic acid, amyl para-aminobenzoate, dihydroxypropyl ethyl para-aminobenzoate, glyceryl para-aminobenzoate, ethyl para-aminobenzoate, octyl para-aminobenzoate, octyldimethyl para-aminobenzoate, ethylene glycol salicylate, octyl salicylate, triethanolamine salicylate, phenyl salicylate, butylphenyl salicylate, benzyl salicylate, homomenthyl salicylate, benzyl cinnamate, octyl para-methoxycinnamate, 2-ethylhexyl para-methoxycinnamate, glyceryl di-para-methoxycinnamate mono-2-ethylhexanoate, isopropyl para-methoxycinnamate, diethanolamine salt of para-methoxyhydrocinnamate, diisopropyl-diisopropylcinnamate mixture, urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenone sulfonic acid and its salts, di Examples include hydroxymethoxybenzophenone, dihydroxymethoxybenzophenone sodium disulfonate, dihydroxybenzophenone, dihydroxydimethoxybenzophenone, hydroxyoctoxybenzophenone, tetrahydroxybenzophenone, butylmethoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2-ethylhexyl-1-oxy)-1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, methyl-O-aminobenzoate, 2-ethylhexyl-2-cyano-3,3-diphenylacrylate, phenylbenzimidazole sulfate, 3-(4-methylbenzylidene)camphor, isopropyldibenzoylmethane, 2-ethylhexyl 4-(3,4-dimethoxyphenylmethylene)-2,5-dioxo-1-imidazolidinepropionate, and their polymer and silane derivatives.

[0044] (5) Examples of powders and pigments: Dyes such as Red No. 104, Red No. 201, Yellow No. 4, Blue No. 1, Black No. 401, lake dyes such as Yellow No. 4 AL Lake and Yellow No. 203 BA Lake, nylon powder, silk powder, urethane powder, Teflon (registered trademark) powder, silicone powder, polymethyl methacrylate powder, cellulose powder, starch, silicone elastomer spherical powder, polymers such as polyethylene powder, yellow iron oxide, red iron oxide, black iron oxide, chromium oxide, carbon black, colored pigments such as ultramarine and iron blue, zinc oxide, titanium oxide, acid Examples of suitable pigments include white pigments such as cerium chloride, extender pigments such as talc, mica, sericite, kaolin, and plate-like barium sulfate, pearl pigments such as titanium dioxide, metal salts such as barium sulfate, calcium carbonate, magnesium carbonate, aluminum silicate, and magnesium silicate, inorganic powders such as silica and alumina, metal soaps such as aluminum stearate, magnesium stearate, zinc palmitate, zinc myristate, magnesium myristate, zinc laurate, and zinc undecylenate, bentonite, smectite, and boron nitride. There are no particular limitations on the shape (spherical, rod-like, needle-like, plate-like, irregular, flaky, spindle-like, etc.) and particle size of these powders. These powders may or may not have been previously surface-treated by conventionally known surface treatments, such as fluorine compound treatment, silicone treatment, silicone resin treatment, pendant treatment, silane coupling agent treatment, titanium coupling agent treatment, oil treatment, N-acylated lysine treatment, polyacrylic acid treatment, metal soap treatment, amino acid treatment, lecithin treatment, inorganic compound treatment, plasma treatment, mechanochemical treatment, etc.

[0045] (6) Examples of surfactants: Anionic surfactants include fatty acid soaps, α-acylsulfonates, alkylsulfonates, alkylarylsulfonates, alkylnaphthalenesulfonates, alkyl sulfates, POE alkyl ether sulfates, alkylamide sulfates, alkyl phosphates, POE alkyl phosphates, alkylamide phosphates, alkyloyl alkyl taurine salts, N-acylamino acid salts, POE alkyl ether carboxylates, alkyl sulfosuccinates, sodium alkylsulfoacetates, acylated hydrolyzed collagen peptide salts, perfluoroalkyl phosphate esters, etc. Cationic surfactants include alkyltrimethylammonium chloride, stearyltrimethylammonium chloride, stearyltrimethylammonium bromide, cetostearyltrimethylammonium chloride, distearyldimethylammonium chloride, stearyldimethylbenzylammonium chloride, behenyltrimethylammonium bromide, benzalkonium chloride, behenic acid amidopropyldimethylhydroxypropylammonium chloride, stearic acid diethylaminoethylamide, stearic acid dimethylaminopropylamide, lanolin derivative quaternary ammonium salts, etc. Amphoteric surfactants include carboxybetaine, amidobetaine, sulfobetaine, hydroxysulfobetaine, amidosulfobetaine, phosphobetaine, aminocarboxylate, imidazoline derivative, and amidoamine types. Nonionic surfactants include propylene glycol fatty acid esters, glycerin fatty acid esters, polyglycerin fatty acid esters, sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE sorbitan fatty acid esters, POE glycerin fatty acid esters, POE alkyl ethers, POE fatty acid esters, POE hydrogenated castor oil, POE castor oil, POE-POP copolymer, POE-POP alkyl ether, polyether-modified silicone lauric acid alkanolamide, alkylamine oxide, and hydrogenated soybean phospholipids. Natural surfactants include lecithin, saponin, and sugar-based surfactants.

[0046] (7) Examples of polyhydric alcohols and sugars include ethylene glycol, diethylene glycol, polyethylene glycol, propylene glycol, dipropylene glycol, polypropylene glycol, glycerin, diglycerin, polyglycerin, 3-methyl-1,3-butanediol, 1,3-butylene glycol, sorbitol, mannitol, raffinose, erythritol, glucose, sucrose, fructose, xylitol, lactose, maltose, maltitol, trehalose, alkylated trehalose, mixed isomerized sugars, sulfated trehalose, pullulan, etc. Chemically modified versions of these can also be used.

[0047] (8) Examples of polymers: acrylic acid ester / methacrylic acid ester copolymer (Plassize, manufactured by GOO Chemical Co., Ltd.), vinyl acetate / crotonic acid copolymer (Resin 28-1310, manufactured by NSC Co., Ltd.), vinyl acetate / crotonic acid / vinyl neodecanoate copolymer (28-2930, manufactured by NSC Co., Ltd.), methyl vinyl ether maleic acid half ester (Gantrez ES, manufactured by ISP Co., Ltd.), t-butyl acrylate / ethyl acrylate / methacrylic acid copolymer (Ruvimer, manufactured by BASF Co., Ltd.), vinylpyrrolidone / vinyl acetate / vinyl propionate copolymer (Ruviscol VAP, manufactured by BASF Co., Ltd.), vinyl acetate Vinyl acetate / crotonic acid copolymer (Rubyset CA, BASF), vinyl acetate / crotonic acid / vinyl pyrrolidone copolymer (Rubyset CAP, BASF), vinyl pyrrolidone / acrylate copolymer (Rubyflex, BASF), acrylate / acrylamide copolymer (Ultrahold, BASF), vinyl acetate / butyl maleate / isobornyl acrylate copolymer (Advantage, ISP), carboxyvinyl polymer (Carbopol, BFGoodrich), acrylic acid / alkyl methacrylate copolymer (Pemulen, BF Examples of such polymers include anionic polymers such as acetic acid amphoteric dialkylaminoethyl methacrylate polymer (Yukaformer, Mitsubishi Chemical), and octylacrylamide acrylate / hydroxypropyl acrylate / butylaminoethyl methacrylate copolymer (AMPHOMER, NSC), cationic polymers such as quaternized vinylpyrrolidone / dimethylaminoethyl methacrylate (GAFQUAT, ISP), and methylvinylimidazolium chloride / vinylpyrrolidone copolymer (Rubycoat, BASF), and nonionic polymers such as polyvinylpyrrolidone (Ruviscol K, BASF), vinylpyrrolidone / vinyl acetate copolymer (Ruviscol VA, BASF), vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer 937, ISP), and vinylcaprolactam / vinylpyrrolidone / dimethylaminoethyl methacrylate copolymer (Copolymer VC713, ISP).In addition, naturally occurring polymeric compounds such as cellulose or derivatives thereof, keratin and collagen or derivatives thereof, calcium alginate, pullulan, agar, gelatin, tamarind seed polysaccharides, xanthan gum, carrageenan, high methoxyl pectin, low methoxyl pectin, guar gum, gum arabic, crystalline cellulose, arabinogalactan, karaya gum, tragacanth gum, alginic acid, albumin, casein, curdlan, gellan gum, and dextran can also be suitably used.

[0048] (9) Examples of physiologically active ingredients: Physiologically active ingredients include substances that impart some kind of physiological activity to the skin when applied to the skin, such as whitening ingredients, immunostimulants, anti-aging agents, UV protection agents, slimming agents, tightening agents, antioxidants, hair growth agents, hair growth promoters, moisturizers, blood circulation promoters, antibacterial agents, disinfectants, drying agents, cooling agents, warming agents, vitamins, amino acids, wound healing promoters, irritation soothing agents, analgesics, cell activators, and enzyme components. Examples of suitable ingredients include angelica extract, avocado extract, hydrangea extract, althaea extract, arnica extract, aloe extract, apricot extract, apricot kernel extract, ginkgo extract, fennel extract, turmeric extract, oolong tea extract, angelica tree extract, echinacea leaf extract, Scutellaria root extract, Phellodendron bark extract, Coptis japonica extract, barley extract, St. John's wort extract, dead nettle extract, watercress extract, orange extract, dried seawater, seaweed extract, hydrolyzed elastin, hydrolyzed wheat powder, hydrolyzed silk, chamomile extract, carrot extract, artemisia capillaris extract, licorice extract, kalkade extract, kakyoku extract, cinchona extract, cucumber extract, guanosine, gardenia extract, bamboo grass extract, sophora flavescens extract, walnut extract, grapefruit extract, clematis extract, chlorella extract, mulberry extract, gentian extract, black tea extract, and yeast extract. , Burdock extract, Fermented rice bran extract, Rice germ oil, Comfrey extract, Collagen, Cowberry extract, Chinese radish extract, Bupleurum extract, Umbilical cord extract, Salvia extract, Soapwort extract, Bamboo shoot extract, Hawthorn extract, Japanese pepper extract, Shiitake mushroom extract, Rehmannia root extract, Lithospermum root extract, Perilla extract, Tilia extract, Spiraea extract, Peony extract, Calamus root extract, Birch extract, Horsetail extract, Ivy extract, Hawthorn extract, elderberry extract, yarrow extract, peppermint extract, sage extract, mallow extract, cnidium extract, Swertia japonica extract, soybean extract, Chinese laurel extract, thyme extract, tea extract, clove extract, Imperata cylindrica extract, tangerine peel extract, Angelica acutiloba extract, calendula extract, peach kernel extract, spruce extract, Houttuynia cordata extract, tomato extract, natto extract, carrot extract, garlic extract,Examples of such extracts include wild rose extract, hibiscus extract, burdock root extract, parsley extract, honey, witch hazel extract, parietaria extract, holly extract, bisabolol, loquat extract, coltsfoot extract, butterbur stalk extract, poria cocos extract, butcher's broom extract, grape extract, propolis, loofah extract, safflower extract, peppermint extract, linden extract, peony extract, hop extract, pine extract, horse chestnut extract, skunk cabbage extract, soapberry extract, melissa extract, peach extract, cornflower extract, eucalyptus extract, saxifrage extract, coix seed extract, mugwort extract, lavender extract, apple extract, lettuce extract, lemon extract, astragalus extract, rose extract, rosemary extract, Roman chamomile extract, and royal jelly extract. In addition, biopolymers such as deoxyribonucleic acid, mucopolysaccharides, sodium hyaluronate, sodium chondroitin sulfate, collagen, elastin, chitin, chitosan, and hydrolyzed eggshell membrane; moisturizing ingredients such as amino acids, hydrolyzed peptides, sodium lactate, urea, sodium pyrrolidone carboxylate, betaine, whey, and trimethylglycine; oily ingredients such as sphingolipids, ceramides, phytosphingosine, cholesterol, cholesterol derivatives, and phospholipids; immunostimulants such as ε-aminocaproic acid, glycyrrhizic acid, β-glycyrrhetinic acid, lysozyme chloride, guaiazulene, and hydrocortisone; vitamin A, vitamin B2, vitamin B6, vitamin C, vitamin D, vitamin E, calcium pantothenate, and bio Vitamins such as cinnamic acid, nicotinamide, and vitamin C ester; active ingredients such as allantoin, diisopropylamine dichloroacetate, and 4-aminomethylcyclohexanecarboxylic acid; antioxidants such as tocopherol, carotenoids, flavonoids, tannins, lignans, and saponins; cell activators such as alpha-hydroxy acids and beta-hydroxy acids; blood circulation promoters such as gamma-oryzanol and vitamin E derivatives; wound healing agents such as retinol and retinol derivatives; whitening agents such as arbutin, kojic acid, placenta extract, sulfur, ellagic acid, linoleic acid, tranexamic acid, and glutathione; cepharanthine, licorice extract, capsicum tincture, hinokitiol, iodized garlic extract, pyridoxine hydrochloride, and DL-alpha-tocopherol.Hair growth promoters such as DL-α-tocopherol acetate, nicotinic acid, nicotinic acid derivatives, calcium pantothenate, D-pantothenyl alcohol, acetyl pantothenyl ethyl ether, biotin, allantoin, isopropyl methylphenol, estradiol, ethinyl estradiol, capronium chloride, benzalkonium chloride, diphenhydramine hydrochloride, tacanal, camphor, salicylic acid, nonylic acid vanillylamide, nonanoic acid vanillylamide, piroctone olamine, glyceryl pentadecanoate, L-menthol, mononitroguaiacol, resorcinol, γ-aminobutyric acid, benzethonium chloride, mexiletine hydrochloride, auxin, female hormones, cantharides tincture, cyclosporine, zinc pyrithione, hydrocortisone, minoxidil, polyoxyethylene sorbitan monostearate, peppermint oil, and Sasanishiki extract are also included.

[0049] (10) Examples of antioxidants include sodium bisulfite, sodium sulfite, erythorbic acid, sodium erythorbate, dilauryl thiodipropionate, tocopherol, tolylbiguanide, nordihydroguaiaretic acid, parahydroxyanisole, butylhydroxyanisole, dibutylhydroxytoluene, ascorbyl stearate, ascorbyl palmitate, octyl gallate, propyl gallate, carotenoids, flavonoids, tannins, lignans, saponins, and plant extracts that are known to have antioxidant effects, such as apple extract and clove extract.

[0050] (11) Examples of solvents include purified water, ethanol, lower alcohols, ethers, LPG, fluorocarbons, N-methylpyrrolidone, fluoroalcohols, volatile linear silicones, and next-generation fluorocarbons.

[0051] Examples of the present invention will be described below. Note that the examples shown below are provided to confirm the various actions and effects of the agent of the present invention obtained by the present invention, and the scope of the present invention is not limited to these products and manufacturing methods.

[0052] Example: Preparation of Yellow Strawberry Guava Extract Yellow strawberry guava fruit (4.61 kg) was crushed, and 99% ethanol (14.25 kg) was added. Extraction was carried out at room temperature (24 hours) with stirring. After filtration at room temperature, the filtrate was concentrated to dryness under reduced pressure to obtain an ethanol extract (540.7 g, yield: 11.7%).

[0053] Test Example 1: Evaluation of the Hair Growth-Promoting Effect of Yellow Strawberry Guava Extract (1) Test Method The experiment was conducted using C3H mice, which have a uniform hair growth cycle. C3H mice have a uniform, synchronized hair growth cycle at a specific age. Therefore, hair removal during the telogen phase can uniformly transition the hair growth cycle to the anagen phase. Ingredients such as minoxidil, which have been shown to promote hair growth in humans, have also been shown to promote hair growth in C3H mice, making them suitable for evaluating hair growth promotion. The test was performed using the method shown in Figure 1. First, 5-week-old C3H mice were pre-housed for one week to allow them to acclimate to the environment (total age: 6 weeks). Then, under isoflurane anesthesia, the backs of the mice were shaved (with electric clippers and a razor) and allowed to rest for two days. The mice were then divided into groups treated with either 5% gum arabic (control group) or 200 mg / kg of the yellow strawberry guava extract described in the above example, and orally administered the test substance for 21 consecutive days (N=8). During this period, the backs of the mice were photographed under isoflurane anesthesia before administration (Day 0), and 13, 19, and 21 days after administration. The results are shown in Figure 2. The area of ​​hair growth was calculated using Image J (free software). The results are shown in Figure 3. Furthermore, 21 days after administration of the test substance, the mice were sacrificed and the backs were shaved. Skin samples were then collected from the shaved areas and stored at -80°C. The protein levels of vascular endothelial growth factor (hereinafter abbreviated as VEGF), which is involved in angiogenesis, metabolism, and increased blood flow, in the collected skin were quantified using Western blotting. The results are shown in Figure 4.

[0054] (2) Measurement Results and Effects of the Example As shown in Figure 2, the control group showed almost no hair growth on Day 13, and even on Day 18, areas without hair growth were noticeable. In contrast, in the group administered with Yellow Strawberry Guava Extract, hair growth began to be noticeable on Day 13, and by Day 18, hair growth had been completed in almost all mice. This confirmed that Yellow Strawberry Guava Extract has a hair growth-promoting effect. Furthermore, as shown in Figure 3, it was confirmed that the hair area value of the mice administered with Yellow Strawberry Guava was significantly increased from Day 13 onwards compared to the control group. Furthermore, as shown in Figure 4, it was confirmed that Yellow Strawberry Guava Extract promoted VEGF production. These results confirm that Yellow Strawberry Guava Extract is useful as a hair growth promoter, especially when administered orally, and that this is due to its VEGF production-promoting effect.

[0055] Test Example 2: Evaluation of the Blood Flow-Increasing Effect of Yellow Strawberry Guava Extract (1) Test Method: Experiments were conducted using C3H mice with a regular hair growth cycle. First, 5-week-old C3H mice were pre-housed for one week to allow them to acclimate to the environment (total age: 6 weeks). The mice were then shaved (using electric clippers and depilatory cream) on their backs under isoflurane anesthesia and allowed to rest for one day. Subsequently, the mice were divided into groups receiving either 5% gum arabic (control group) or 200 mg / kg of the Yellow Strawberry Guava Extract described in the above example, and administered the test substance for seven consecutive days (N=6). During this period, blood flow videos were recorded on the backs under isoflurane anesthesia before administration (Day 0) and 1, 4, and 7 days after administration. Blood flow videos (see Figure 5) were captured at two locations on the upper and lower backs of the mice using a TOKU Capillaro blood flowscope (Toku Seiki). Blood flow velocity was then calculated from the videos using the vascular blood flow image analysis software "Capimetrics." For the analysis, the average value of two to three blood vessels in the video was calculated and used.

[0056] (2) Measurement Results and Effects of the Example As shown in Figure 6, the group administered 200 mg / kg of the Yellow Strawberry Guava Extract showed an increased blood flow rate on the upper back of the mice on the first day of administration compared to the control group. There was also a tendency for the blood flow rate on the lower back to increase (P=0.060). These results demonstrate that the Yellow Strawberry Guava Extract has the effect of increasing blood flow on the back of the mice.

[0057] The following are formulation examples of the hair growth promoter (yellow strawberry guava extract) according to the present invention. Note that the following formulation examples do not limit the present invention.

[0058] Formulation example 1: Chewing gum Sugar 53.0 wt% Gum base 20.0 Glucose 10.0 Starch syrup 16.0 Flavoring 0.5 Hair growth promoter 0.5 100.0 wt%

[0059] Formulation Example 2: Gummy Reduced starch syrup 40.0 wt% Granulated sugar 20.0 Glucose 20.0 Gelatin 4.7 Water 9.68 Kiwi juice 4.0 Kiwi flavor 0.6 Coloring 0.02 Hair growth promoter 1.0 100.0 wt%

[0060] Formulation Example 3: Candy Sugar 50.0 wt% Starch syrup 33.0 Water 14.4 Organic acid 2.0 Flavoring 0.2 Hair growth promoter 0.4 100.0 wt%

[0061] Formulation example 4: Yogurt (hard / soft) Milk 41.5 wt% Skim milk powder 5.8 Sugar 8.0 Agar 0.15 Gelatin 0.1 Lactic acid bacteria 0.005 Hair growth promoter 0.4 Fragrance Trace amount Water Remainder 100.0 wt%

[0062] Formulation Example 5: Soft capsule Kiwi seed oil 87.0 wt% Emulsifier 12.0 Hair growth promoter 1.0 100.0 wt%

[0063] Formulation Example 6: Coffee drink (liquid) Roasted coffee beans 6.0 wt% Sugar 6.0 Baking soda 0.2 Emulsifier 0.15 Hair growth promoter 1.0 Water Remaining 100.0 wt%

[0064] Formulation Example 7: Coffee drink (powder) Instant coffee 90.0 wt% Skim milk 7.0 Hair growth promoter 3.0 100.0 wt%

[0065] Formulation Example 8: Soft drink Fructose glucose syrup 30.0 wt% Emulsifier 0.5 Hair growth promoter 0.05 Flavoring as needed Purified water Remainder 100.0 wt%

[0066] Formulation Example 9: Tablet Lactose 54.0 wt% Microcrystalline cellulose 30.0 Starch hydrolysate 10.0 Glycerin fatty acid ester 5.0 Hair growth promoter 1.0 100.0 wt%

[0067] Formulation Example 10: Tablets Sugar 76.4 wt% Glucose 19.0 Sucrose fatty acid ester 0.2 Hair growth promoter 0.5 Purified water 3.9 100.0 wt%

[0068] Formulation Example 11: Hair growth agent (pharmaceutical grade) Hair growth promoter 5.5 wt% Tocopherol acetate 0.1 Pantothenyl alcohol 0.2 Dipotassium glycyrrhizinate 0.1 Polyoxyethylene (EO60) hydrogenated castor oil 0.3 Fragrance 0.1 Propylene glycol 2.0 Ethanol 60.0 Purified water Balance 100.0 wt%

[0069] Formulation Example 12: Hair tonic Ethanol 60.0 wt% Polyoxyethylene hydrogenated castor oil (E.O. 60 mol) 0.5 Glycerin 3.0 Menthol 0.2 Hair growth promoter 0.3 Fragrance and colorant appropriate amount Ion-exchanged water Remainder 100.0 wt%

[0070] Formulation Example 13: Shampoo Sodium polyoxyethylene alkyl ether sulfate (E.O. 2 moles) 15.0 wt% Coconut oil fatty diethanolamide 5.0 Glycerin 3.0 Hair growth promoter 0.4 Ethanol 5.0 Fragrance and preservative appropriate amount Ion-exchanged water Remainder 100.0 wt%

[0071] Formulation Example 14: Hair Cream Liquid paraffin 20.0 wt% Solid paraffin 3.0 Polyoxyethylene cetyl ether (E.O. 15 moles) 2.0 Sorbitan sesquioleate 1.0 Hair growth promoter 0.2 Ethanol 10.0 Potassium hydroxide 0.1 Glycerin 3.0 Fragrance and preservative appropriate amount Vaseline Remainder 100.0 wt%

[0072] Formulation Example 15: Ointment White beeswax 5.0 wt% Purified lanolin 5.0 Hair growth promoter 1.0 Fragrance 0.1 Vaseline Balance 100.0 wt%

[0073] As described above, the present invention can provide a hair growth promoter.

Claims

1. A hair growth promoter containing yellow strawberry guava fruit extract as the active ingredient.

2. A vascular endothelial cell growth factor production promoter containing yellow strawberry guava fruit extract as the active ingredient.

3. A food or drink composition for promoting hair growth, containing the hair growth promoter according to claim 1 as an active ingredient.

4. A pharmaceutical composition for promoting hair growth, comprising the hair growth promoter according to claim 1 as an active ingredient.

5. A hair cosmetic composition for promoting hair growth, containing the hair growth promoter according to claim 1 as an active ingredient.

6. A composition for external application to the skin for promoting hair growth, containing the hair growth promoter according to claim 1 as an active ingredient.

7. A hair growth promoter for oral administration, containing a hydroethanolic extract of yellow strawberry guava fruit as the active ingredient.

8. A method for promoting hair growth in a human, comprising: a) obtaining a polar solvent extract from a raw material of yellow strawberry guava fruit; and b) administering an effective amount of the polar solvent extract to a human by oral or parenteral administration to promote the production of vascular endothelial growth factor in the human.

Images