Collagen treatment structure, system and method

Abstract

A collagen treatment structure (10), system and method. The collagen treatment structure (10) comprises a treatment cavity (11) and a centrifugal treatment mechanism (12). The treatment cavity (11) is provided with a discharge port (111). The centrifugal treatment mechanism (12) comprises centrifugal ultrafiltration members (122) and a centrifugal driving member (121) connected thereto, wherein the centrifugal ultrafiltration members (122) are driven by the centrifugal driving member (121) to rotate; the centrifugal ultrafiltration members (122) are arranged in the treatment cavity (11); each centrifugal ultrafiltration member (122) is provided with ultrafiltration membrane layers (1221) on two opposite surfaces; a filtering cavity (1222) is provided inside each centrifugal ultrafiltration member (122); and each centrifugal ultrafiltration member (122) is further provided with a waste liquid hole (1223) in communication with the filtering cavity (1222), and the waste liquid holes (1223) are in communication with the discharge port (111). By combining the principles of centrifugation and cross-flow filtration, and under the action of a centrifugal force, membrane-surface filter layers are efficiently removed, the membrane-surface concentration difference is eliminated, and a membrane blockage phenomenon does not easily occur, such that the purity of collagen is improved, and the collagen concentration can reach 20 mg / ml.

Description

Collagen processing structures, systems and methods

[0001] This application claims priority to Chinese Patent Application No. 2024105175701, filed on April 26, 2024, entitled "Collagen Processing Structure, System and Method", the entire contents of which are incorporated herein by reference. [Technical Field]

[0002] This disclosure relates to the field of biomaterials technology, and in particular to a collagen processing structure, system and method. [Background Technology]

[0003] Collagen is a large protein molecule composed of three chains, characterized by high viscosity, poor water solubility, and a tendency to gel. Its viscosity increases rapidly, especially during purification and concentration, making it very difficult to achieve and process high concentrations (e.g., exceeding 20 mg / ml) of collagen. Currently, collagen production typically employs methods such as salting out, dialysis, ultrafiltration, and a combination of these methods for purification and concentration. However, these methods for purifying and concentrating high-concentration collagen suffer from drawbacks including low purity, low efficiency, susceptibility to contamination, and technical complexity.

[0004] [Summary of the Invention]

[0005] One technical solution adopted in this application is: providing a collagen processing structure, which includes a processing chamber and a centrifugal processing mechanism. The processing chamber is provided with a discharge port. The centrifugal processing mechanism includes a centrifugal drive and a centrifugal ultrafiltration element. The centrifugal ultrafiltration element is connected to the centrifugal drive and rotates under the centrifugal drive. The centrifugal ultrafiltration element is disposed in the processing chamber, and ultrafiltration membrane layers are provided on opposite sides of the centrifugal ultrafiltration element. The centrifugal ultrafiltration element has a filtration chamber inside, and the centrifugal ultrafiltration element also has a waste liquid hole communicating with the filtration chamber, and the waste liquid hole is connected with the discharge port.

[0006] The centrifugal drive unit includes a hollow centrifugal shaft that extends into the processing chamber. The centrifugal shaft has a hollow channel, and the end away from the centrifugal drive unit is connected to the discharge port. Each centrifugal ultrafiltration unit has a mounting hole, and the centrifugal shaft is fixed in the mounting hole. The waste liquid hole penetrates the inner wall of the mounting hole. The centrifugal shaft has a liquid inlet, which communicates with the waste liquid hole.

[0007] Each mounting hole is located at the center of the corresponding centrifugal ultrafiltration element.

[0008] Each centrifugal ultrafiltration element is disc-shaped, with the ultrafiltration membrane layer located on opposite sides of the disc, and the mounting hole is at the center of the disc.

[0009] The processing chamber also includes a feed inlet and a return outlet. Along the direction of gravity, both the discharge outlet and the feed inlet are located below the return outlet.

[0010] The pore size of each ultrafiltration membrane layer ranges from 10nm to 30nm.

[0011] This application also includes a second technical solution, providing a collagen processing system, including the aforementioned collagen processing structure, storage tank, delivery pipeline, return pipeline, sewage discharge pipeline and water injection pipeline; the delivery pipeline connects the storage tank and the collagen processing structure; the return pipeline connects the collagen processing structure and the storage tank; the sewage discharge pipeline connects to the discharge outlet; and the water injection pipeline connects to the storage tank.

[0012] The water injection pipeline can control two working states: shutting off water injection or injecting water into the storage tank. When it is in the working state of injecting water into the storage tank, the amount of water injected into the storage tank is equal to the amount of waste liquid discharged from the outlet.

[0013] The collagen processing system also includes a pressure regulating component, which consists of a compressed air inlet and an ultrafiltration pump. The compressed air inlet is connected to a storage tank and used to regulate the pressure inside the storage tank. One end of the ultrafiltration pump is connected to the storage tank, and the other end is connected to the collagen processing structure.

[0014] The collagen processing system also includes a rinsing tank and rinsing pipelines, with the rinsing pipelines connecting the collagen processing structure and the rinsing tank.

[0015] This application also includes a third technical solution, providing a method for processing collagen, comprising: conveying an initial collagen extract from a storage tank to a collagen processing structure; performing cyclic centrifugal ultrafiltration treatment through a centrifugal processing mechanism within the collagen processing structure to purify the initial collagen extract; and stopping the purification of the initial collagen extract in response to the conductivity of the collagen solution in the storage tank meeting a first preset value; wherein, performing cyclic centrifugal ultrafiltration treatment through a centrifugal processing mechanism within the collagen processing structure to purify the initial collagen extract includes: discharging waste liquid generated in each centrifugal ultrafiltration treatment; returning the collagen solution extracted in each centrifugal ultrafiltration treatment to the storage tank, and simultaneously replenishing the storage tank with the same amount of water as the waste liquid; the mixture formed by the remaining initial collagen extract in the storage tank, the replenished water, and the collagen solution extracted in the centrifugal ultrafiltration treatment is again conveyed to the collagen processing structure for cyclic centrifugal ultrafiltration treatment, and the cycle continues until the purification of the initial collagen extract is stopped.

[0016] The process includes, after stopping the purification of the initial collagen extract, a concentration treatment of the purified initial collagen extract, including: continuing to transport the purified initial collagen extract from the storage tank to the collagen processing structure; the centrifugal processing mechanism in the collagen processing structure performing cyclic centrifugal ultrafiltration to concentrate the purified initial collagen extract, and during the cyclic centrifugal ultrafiltration to concentrate the purified initial collagen extract, stopping the replenishment of water to the storage tank, and discharging the waste liquid generated in each centrifugal ultrafiltration treatment, and returning the collagen liquid extracted by the centrifugal ultrafiltration treatment to the storage tank; the mixture formed by the remaining purified initial collagen extract in the storage tank and the collagen liquid extracted by the centrifugal ultrafiltration treatment is transported back to the collagen processing structure for centrifugal ultrafiltration treatment again, and the cycle is repeated until the centrifugal ultrafiltration treatment is stopped; in response to the total amount of waste liquid discharged during the concentration treatment reaching a second preset value, the centrifugal ultrafiltration treatment is stopped, and the collagen concentrate in the storage tank and the collagen processing structure is collected.

[0017] During the process of purifying the initial collagen extract through cyclic centrifugal ultrafiltration in the centrifugal processing unit: the rotation speed of the centrifugal processing unit is 100-500 rpm; the feed pressure of the processing chamber is 0.1-0.3 MPa; and the discharge pressure of the processing chamber is 0.01-0.3 MPa.

[0018] During the concentration process of the purified initial collagen extract: the centrifuge speed is 500-800 rpm, the feed pressure of the processing chamber is 0.1-0.5 MPa, and the discharge pressure of the processing chamber is 0.05-0.5 MPa.

[0019] The process of collecting collagen concentrate in the storage tank and collagen processing structure includes: adjusting the pressure in the storage tank to 0.2-0.3 MPa, collecting collagen concentrate from the first outlet of the storage tank, and closing the first outlet after collection; adjusting the centrifugal processing mechanism to rotate at a speed of 200-300 rpm, and opening the second outlet of the collagen processing structure to collect collagen concentrate.

[0020] The process includes, after collecting the collagen concentrate from the storage tank and the collagen processing structure, rinsing the collagen processing structure, including closing the delivery pipeline between the collagen processing structure and the storage tank, and closing the drain pipeline; connecting the rinsing pipeline to the collagen processing structure; turning on the rinsing pump on the rinsing pipeline, adjusting the inlet pressure of the discharge port of the processing chamber to 0.1-0.2 MPa, and adjusting the speed of the centrifugal processing mechanism to 200-300 rpm for cleaning.

[0021] The above description is only an overview of the technical solution of this application. In order to better understand the technical means of this application and to implement it in accordance with the contents of the specification, and to make the above and other objects, features and advantages of this application more obvious and understandable, the following are specific embodiments of this application. [Attached Image Description]

[0022] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiments below. The accompanying drawings are for illustrative purposes only and are not intended to limit the scope of this application. Furthermore, the same reference numerals denote the same parts throughout the drawings. In the drawings:

[0023] Figure 1 is a schematic diagram of the collagen treatment structure shown in some embodiments of this specification;

[0024] Figure 2 is a first structural schematic diagram of a centrifugal ultrafiltration element for collagen treatment according to some embodiments of this specification;

[0025] Figure 3 is a schematic diagram of the second structure of a centrifugal ultrafiltration element for collagen treatment according to some embodiments of this specification;

[0026] Figure 4 is a schematic diagram of the structure of a collagen processing system according to some embodiments of this specification;

[0027] Figure 5 is a schematic diagram of the purification process of collagen treatment methods according to some embodiments of this specification;

[0028] Figure 6 is a schematic diagram of the concentration process of a collagen processing method according to some embodiments of this specification.

[0029] The reference numerals in the detailed embodiments are as follows: 100, Collagen processing system; 10, Collagen processing structure; 11, Processing tank; 111, Discharge port; 112, Feed inlet; 113, Return port; 12, Centrifugal processing mechanism; 121, Centrifugal drive component; 1211, Centrifugal shaft; 1212, Hollow channel; 122, Centrifugal filter component; 1221, Filter membrane; 1222, Filter chamber; 1223, Waste liquid hole; 1224, Mounting hole; 13, Second discharge port; 20, Storage tank; 21, First discharge port; 30, Conveying pipeline; 40, Return pipeline; 50, Sewage discharge pipeline; 60, Water injection pipeline; 61, Flow meter; 70, Pressure regulating component; 72, Ultrafiltration pump; 73, Flushing pump; 71, Compressed air inlet; 80, Flushing tank; 90, Flushing pipeline.

Detailed Implementation Methods

[0030] The embodiments of the technical solution of this application will be described in detail below. The following embodiments are only used to illustrate the technical solution of this application more clearly, and are therefore only examples, and should not be used to limit the scope of protection of this application.

[0031] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application and the above-mentioned figure descriptions are intended to cover non-exclusive inclusions.

[0032] In the description of the embodiments of this application, technical terms such as "first" and "second" are used only to distinguish different objects and should not be construed as indicating or implying relative importance or implicitly specifying the number, specific order, or primary and secondary relationship of the indicated technical features. In the description of the embodiments of this application, unless otherwise explicitly specified, the term "multiple" refers to two or more (including two), similarly, "multiple sets" refers to two or more (including two sets), and "multiple pieces" refers to two or more (including two pieces).

[0033] In this document, the term "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0034] In the description of the embodiments of this application, the term "and / or" is simply a description of the association relationship between associated objects, indicating that three relationships can exist. For example, A and / or B can represent the following three situations: A exists alone, A and B exist simultaneously, and B exists alone. In addition, the character " / " in this document generally indicates that the associated objects are in an "or" relationship.

[0035] In the description of the embodiments of this application, the technical terms "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing the embodiments of this application and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on the embodiments of this application.

[0036] In the description of the embodiments of this application, unless otherwise expressly specified and limited, technical terms such as "installation," "connection," "joining," and "fixing" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. For those skilled in the art, the specific meaning of the above terms in the embodiments of this application can be understood according to the specific circumstances.

[0037] Animal-derived collagen is mainly derived from animal tissues such as bones, skin, tendons, and membranes. Among them, type I collagen is extremely abundant, accounting for about 80%-90% of the total collagen. As a major component of the extracellular matrix, it has good repair properties, biocompatibility, and biodegradability, and is widely used in medicine, food, and cosmetic fields, playing an important role.

[0038] Collagen is generally produced by first extracting collagen from tissues using physical, chemical, and biological methods (such as the common acid-enzyme extraction method), followed by further purification and concentration. Currently, collagen production typically employs methods such as salting out, dialysis, ultrafiltration, and a combination of these methods for purification and concentration.

[0039] However, collagen is a large protein molecule composed of three chains, characterized by high viscosity, poor water solubility, and easy gelation. Its viscosity increases rapidly, especially during purification and concentration, making it very difficult to obtain high concentrations (e.g., exceeding 20 mg / ml) of collagen. The methods mentioned above for purifying and concentrating high-concentration collagen suffer from drawbacks such as low purity, low efficiency, susceptibility to contamination, and technical complexity.

[0040] Based on the above considerations, in order to solve the technical problems existing in the methods for purifying and concentrating collagen in the prior art, this application proposes a collagen processing structure, system and method. This collagen processing structure, system and method can improve the purity of collagen, improve the ultrafiltration efficiency, is technically simple, has a high concentration factor, and the collagen concentration can reach 20mg / ml.

[0041] For ease of explanation, the following embodiments use a collagen processing structure from one embodiment of this application as an example.

[0042] Please refer to Figures 1 to 3. Figure 1 is a schematic diagram of the collagen processing structure according to some embodiments of this specification; Figure 2 is a first schematic diagram of the centrifugal ultrafiltration element of the collagen processing structure according to some embodiments of this specification; Figure 3 is a second schematic diagram of the centrifugal ultrafiltration element of the collagen processing structure according to some embodiments of this specification. In one aspect of this application, a collagen processing structure 10 is provided, which includes a processing chamber 11 and a centrifugal processing mechanism 12. The processing chamber 11 is provided with a discharge port 111. The centrifugal processing mechanism 12 includes a centrifugal drive 121 and a centrifugal ultrafiltration element 122. The centrifugal ultrafiltration element 122 is connected to the centrifugal drive 121 and rotates under the centrifugal drive 121. Specifically, the centrifugal ultrafiltration element 122 is disposed in the processing chamber 11, and ultrafiltration membrane layers 1221 are provided on opposite sides of the centrifugal ultrafiltration element 122. A filtration chamber 1222 is provided inside the centrifugal ultrafiltration element 122. The centrifugal ultrafiltration element 122 is also provided with a waste liquid hole 1223 that communicates with the filter chamber 1222 and the waste liquid hole 1223 is connected to the discharge port 111.

[0043] As can be seen from the above structure, after the collagen extract enters the processing chamber 11, the small molecule impurities in the collagen extract are filtered through the ultrafiltration membrane layer 1221. The impurities enter the inner filtration chamber 1222 from the outside of the ultrafiltration membrane layer 1221, and are then discharged from the discharge port 111 through the waste liquid hole 1223, while the collagen molecular structure is retained in the processing chamber 11. Furthermore, the collagen processing structure 10 of this application embodiment adopts a rotary structure that is completely different from the cross-flow ultrafiltration in related technologies. Combining the principles of centrifugation and cross-flow filtration, on the one hand, the centrifugal drive 121 drives the centrifugal ultrafiltration element 122 to rotate, and the centrifugal force is used to separate the fluid into high and low concentrations. The different linear velocities of the inner and outer rings provide different shear forces to deal with the feed liquid, thereby improving the ultrafiltration efficiency. The centrifugal motion forms a certain surface flow velocity on the surface of the centrifugal ultrafiltration element 122 to achieve cross-flow filtration due to the different linear velocities of the inner and outer rings. This allows substances smaller than the pore size of the ultrafiltration membrane layer 1221 to pass through and be filtered out, while collagen molecules larger than the pore size of the ultrafiltration membrane layer 1221 are retained, thereby achieving the ultrafiltration purification of collagen. On the other hand, the centrifugal force generated by the rotation of the centrifugal ultrafiltration element 122 and the strong turbulence generated by the collagen extract due to different linear velocities during ultrafiltration can improve the efficiency of removing the filter layer on the surface of the ultrafiltration membrane 1221, eliminate the concentration difference on the surface of the ultrafiltration membrane 1221, reduce the occurrence of membrane blockage, thereby improving the purity of collagen and improving ultrafiltration efficiency.

[0044] Furthermore, this application utilizes centrifugal force to separate collagen extract into high and low concentrations, which is a simple technique. Compared with existing technologies, it does not rely on high circulation flow and operating pressure provided by adjusting pressure. Therefore, the shear force between the centrifugal ultrafiltration element 122 and the collagen extract is relatively weak, which is beneficial to protecting the structure of collagen.

[0045] In one specific embodiment, the ultrafiltration membrane layer 1221 can be made of inorganic ceramic material. Inorganic ceramic material has strong resistance to acids, alkalis, and heat, is easy to clean and maintain, and can extend the service life of the ultrafiltration membrane layer 1221 and reduce contamination. In another specific embodiment, the ultrafiltration membrane layer 1221 can also be made of other materials, which will not be described in detail here.

[0046] Furthermore, the centrifugal drive component 121 includes a hollow centrifugal shaft 1211 extending into the processing chamber 11. The centrifugal shaft 1211 has a hollow channel 1212, and one end away from the centrifugal drive component 121 is connected to the discharge port 111. Each centrifugal ultrafiltration element 122 has a mounting hole 1224, and the centrifugal shaft 1211 is fixed in the mounting hole 1224. The waste liquid hole 1223 penetrates the inner wall of the mounting hole 1224. The centrifugal shaft 1211 has a liquid inlet (not shown in the figure), which communicates with the waste liquid hole 1223. With the above structure, the centrifugal shaft 1211 can be driven to rotate, thereby driving the centrifugal ultrafiltration element 122 to rotate.

[0047] In one embodiment, the number of centrifugal ultrafiltration elements 122 can be several. Several centrifugal ultrafiltration elements 122 are arranged at intervals on the centrifugal shaft 1211 and rotate under the drive of the centrifugal shaft 1211 to form cross-flow, thereby performing cross-flow filtration on the collagen extract. Under the action of centrifugal force, they move from the end of the filter chamber 1222 away from the centrifugal shaft 1211 to the hollow channel 1212 and are discharged into the hollow channel 1212, thereby improving the ultrafiltration efficiency. Each centrifugal ultrafiltration element 122 is installed on the centrifugal shaft 1211 through the mounting hole 1224, and the filtration chamber 1222 is connected to the hollow channel 1212. This allows for the removal of impurities from the collagen extract. Impurities filtered by the ultrafiltration membrane layer 1221 enter the filtration chamber 1222, then enter the hollow channel 1212 through the waste liquid hole 1223 and the liquid inlet, and finally exit through the discharge port 111. This achieves the purification of collagen within the collagen treatment structure 10, retaining the collagen molecular structure, removing other impurities, and improving the purity of the collagen.

[0048] Furthermore, each mounting hole 1224 is located at the center of the corresponding centrifugal ultrafiltration element 122, which can reduce the radius of rotation of the centrifugal ultrafiltration element 122 around the centrifugal shaft 1211, that is, rotate with the distance from the center to the edge of the centrifugal ultrafiltration element 122 as the radius, which can reduce the space occupied by the centrifugal ultrafiltration element 122 and save structural costs.

[0049] In one specific embodiment, each centrifugal ultrafiltration element 122 is disc-shaped, with ultrafiltration membrane layers 1221 located on opposite sides of the disc, and mounting holes 1224 being the center of the disc. That is, the centrifugal ultrafiltration element 122 rotates around the centrifugal shaft 1211 with its radius, thereby improving ultrafiltration efficiency.

[0050] Furthermore, the processing chamber 11 also includes an inlet 112 and a return outlet 113. Along the direction of gravity, both the outlet 111 and the inlet 112 are located below the return outlet 113. The collagen extract enters the processing chamber 11 through the inlet 112. On one hand, the ultrafiltered collagen structure enters the return outlet 113 in the opposite direction of gravity. On the other hand, the filtered impurities enter the filtration chamber 1222 and are discharged from the outlet 111 along the direction of gravity, thus ensuring more thorough ultrafiltration of the collagen.

[0051] In one specific embodiment, the pore size range of each ultrafiltration membrane layer 1221 is 10nm-30nm, such as 10nm, 15nm, 20nm, 25nm and 30nm, etc., to separate collagen liquid and small molecule impurities by ultrafiltration of collagen extract.

[0052] Please refer to Figure 4, which is a schematic diagram of the structure of a collagen processing system according to some embodiments of this specification. In another aspect, this application provides a collagen processing system 100, including the aforementioned collagen processing structure 10, a storage tank 20, a delivery pipeline 30, a return pipeline 40, a drain pipeline 50, and a water injection pipeline 60. The delivery pipeline 30 connects the storage tank 20 and the collagen processing structure 10; the return pipeline 40 connects the collagen processing structure 10 and the storage tank 20; the drain pipeline 50 connects to a discharge port 111; and the water injection pipeline 60 connects to the storage tank 20. Further, the collagen processing system 100 also includes a pressure regulating component 70, which includes a compressed air inlet 71 and an ultrafiltration pump 72. The compressed air inlet 71 is connected to the storage tank 20 and is used to regulate the pressure within the storage tank 20. The ultrafiltration pump 72 is connected to the storage tank 20 at one end and the collagen processing structure 10 at the other end, and is used to provide power to transport the material in the storage tank 20 to the collagen processing structure 10.

[0053] Specifically, storage tank 20 is used to store materials, such as water, collagen extract, and ultrafiltered collagen solution. Delivery pipeline 30 is used to transport the materials in storage tank 20 to collagen processing structure 10. In another embodiment, an ultrafiltration pump 72 is installed on delivery pipeline 30, with one end connected to storage tank 20 and the other end connected to collagen processing structure 10. This pump can regulate the pressure within storage tank 20 and collagen processing structure 10 to ensure smooth liquid output. Return pipeline 40 is used to return the ultrafiltered collagen solution from centrifugal processing unit 12 to storage tank 20 for circulating ultrafiltration. In another embodiment, pressure regulating component 70 may also include a return pump installed on return pipeline 40 to regulate the return pressure and ensure liquid return. In other embodiments, the pressure regulating component may be located in other positions to increase or decrease pressure according to different stages. Drainage pipeline 50 is used to discharge filtered impurities. Water injection pipe 60 is used to inject water into storage tank 20 to maintain the material balance in storage tank 20. It should be noted that the entire collagen processing system 100 includes two stages:

[0054] Purification Stage: The initial collagen extract from storage tank 20 is transferred to collagen processing structure 10. Centrifugation unit 12 in collagen processing structure 10 performs ultrafiltration on the initial collagen extract. The ultrafiltered collagen solution is returned to storage tank 20 via reflux line 40. The waste liquid containing impurities after filtration is discharged through drain line 50. An equal volume of water for injection is injected into storage tank 20 through water injection line 60 according to the waste liquid discharge volume. Ultrafiltration continues until purification is complete. After purification, concentration can be performed directly without changing equipment or adding concentration reagents, making the operation simple.

[0055] Concentration stage: Close the water supply line 60, continue to transport the collagen liquid in the storage tank 20 to the collagen processing structure 10, the ultrafiltration collagen flows back to the storage tank 20 through the return line 40, and the filtrate is discharged through the drain line 50. Repeat the cycle until the concentration is completed.

[0056] In one embodiment, the water injection pipeline 60 can be controlled to operate in two states: shutting off water injection or injecting water into the storage tank 20. In one specific embodiment, the water injection pipeline 60 can be controlled to be in the shut-off state to proceed with the next stage of operation, such as shutting off water injection after purification to proceed with concentration. In another specific embodiment, the water injection pipeline 60 can also be controlled to be in the water injection state into the storage tank 20. In this case, flow meters 61 can be added to both the water injection pipeline 60 and the sewage discharge pipeline 50 to calculate the liquid flow rate, ensuring that the amount of water injected into the storage tank 20 equals the amount of waste liquid discharged from the discharge port 111, thereby maintaining the material balance in the storage tank 20. Furthermore, water can be automatically added based on the amount of waste liquid discharged from the discharge port 111 to achieve automation.

[0057] Furthermore, the collagen treatment system 100 also includes a rinsing tank 80 and a rinsing pipeline 90, with the rinsing pipeline 90 connecting the collagen treatment structure 10 and the rinsing tank 80. The rinsing tank 80 can be filled with a cleaning agent, which is then delivered to the collagen treatment structure 10 via the rinsing pipeline 90 to clean the collagen treatment structure 10. In one specific embodiment, the pressure regulating component 70 may further include a rinsing pump 73, which can be installed on the rinsing pipeline 90 to provide power to deliver the cleaning agent from the rinsing tank 80 to the collagen treatment structure 10 to regulate the pressure of the rinsing pipeline 90. This design utilizes the backflushing principle, which facilitates system cleaning, extends the membrane's service life, and reduces membrane fouling.

[0058] Please refer to Figure 5, which is a schematic diagram of the purification process of a collagen processing method according to some embodiments of this specification. In another aspect, this application provides a method for processing collagen, comprising:

[0059] S110, the initial collagen extract in storage tank 20 is transported to collagen processing structure 10.

[0060] Specifically, the pressure can be adjusted by the ultrafiltration pump 72 of the pressure regulating component 70, and the feed pressure of the initial collagen extract into the collagen treatment structure 10 can be set to 0.1-0.3 MPa, such as 0.1 MPa, 0.2 MPa and 0.3 MPa, to ensure that the initial collagen extract is smoothly delivered to the collagen treatment structure 10.

[0061] S120, the initial collagen extract is purified by cyclic centrifugation and ultrafiltration through the centrifugation processing mechanism 12 within the collagen processing structure 10.

[0062] Specifically, during the purification of the initial collagen extract through circulating centrifugal ultrafiltration in the centrifugal processing unit 12: the rotation speed of the centrifugal processing unit 12 can be set to 100-500 rpm, such as 200 rpm, 300 rpm, 400 rpm, and 500 rpm, to improve ultrafiltration efficiency. The discharge pressure of the processing chamber 11 can be set to 0.01 MPa-0.3 MPa, such as 0.01 MPa, 0.05 MPa, 0.10 MPa, 0.15 MPa, 0.20 MPa, 0.25 MPa, and 0.30 MPa, to ensure that the ultrafiltered collagen extract can be smoothly discharged.

[0063] Furthermore, the purification of the initial collagen extract through cyclic centrifugal ultrafiltration via the centrifugal processing mechanism 12 within the collagen processing structure 10 specifically includes the following processes: discharging the waste liquid generated during each centrifugal ultrafiltration process; returning the collagen liquid extracted during each centrifugal ultrafiltration process to the storage tank 20, while simultaneously replenishing the storage tank 20 with the same amount of water as the waste liquid; the mixture formed by the remaining initial collagen extract, replenished water, and collagen liquid extracted during centrifugal ultrafiltration in the storage tank 20 is then transported back to the collagen processing structure 10 for cyclic centrifugal ultrafiltration, and this cycle continues until the purification of the initial collagen extract is stopped. Specifically, the waste liquid containing impurities generated during the centrifugal ultrafiltration process enters the filter chamber 1222, and under the action of centrifugal force, it enters the hollow channel 1212 through the waste liquid hole 1223 and the liquid inlet, and is finally discharged through the drain pipe 50. The collagen molecules after ultrafiltration remain in the processing chamber 11 and are mixed with the liquid and returned to the storage tank 20. The waste liquid containing impurities is discharged through the drain pipe 50. In order to maintain the material balance in the storage tank 20, a certain amount of waste liquid is discharged, that is, a certain amount of water for injection is injected.

[0064] S130, in response to the conductivity of the collagen solution in the storage tank 20 meeting the first preset value, the purification of the initial collagen extract is stopped.

[0065] Specifically, purification ends when the conductivity of the collagen solution in storage tank 20 meets a first preset value. In one specific embodiment, the first preset value can be set to 50 μS / cm, that is, purification ends when the conductivity of the collagen solution is ≤50 μS / cm.

[0066] Please refer to Figures 4 and 6. Figure 6 is a schematic diagram of the concentration process of a collagen processing method according to some embodiments of this specification. Further, after stopping the purification of the initial collagen extract, the method further includes a concentration process of the purified initial collagen extract, specifically including:

[0067] S210, continue to transport the purified initial collagen extract from storage tank 20 to collagen processing structure 10.

[0068] Specifically, to concentrate the collagen solution and increase the collagen concentration, the purified initial collagen extract in storage tank 20 is further transported to collagen processing structure 10 for ultrafiltration. The feed pressure of processing chamber 11 can be set to 0.1-0.5 MPa, for example, 0.1 MPa, 0.2 MPa, 0.3 MPa, 0.4 MPa, 0.5 MPa, etc., to ensure that the purified initial collagen extract is smoothly transported to collagen processing structure 10.

[0069] S220, the centrifugal processing mechanism 12 in the collagen processing structure 10 performs cyclic centrifugal ultrafiltration to concentrate and purify the initial collagen extract. During the cyclic centrifugal ultrafiltration to concentrate and purify the initial collagen extract, water is stopped from being added to the storage tank 20, and the waste liquid generated in each centrifugal ultrafiltration is discharged. The collagen liquid extracted by centrifugal ultrafiltration is returned to the storage tank 20.

[0070] Specifically, stopping the replenishment of water to storage tank 20 is to further concentrate the purified initial collagen extract and increase the collagen concentration. During the concentration process of the purified initial collagen extract: the rotation speed of centrifuge 12 can be set to 500-800 rpm, such as 500 rpm, 600 rpm, 700 rpm, 800 rpm, etc., and the discharge pressure of processing chamber 11 can be set to 0.05-0.5 MPa, such as 0.05 MPa, 0.15 MPa, 0.25 MPa, 0.35 MPa, 0.45 MPa, 0.5 MPa, etc., to improve ultrafiltration efficiency, reduce shear force, and protect the collagen molecular structure.

[0071] S230, the mixture of the remaining purified initial collagen extract and the collagen solution extracted by centrifugal ultrafiltration in the storage tank 20 is transported back to the processing chamber for centrifugal ultrafiltration again, and the process is circulated until the centrifugal ultrafiltration is stopped.

[0072] Specifically, by continuously circulating ultrafiltration to further concentrate the mixture formed by the remaining purified initial collagen extract in storage tank 20 and the collagen solution extracted by centrifugal ultrafiltration, the concentration of collagen can be increased.

[0073] S240, in response to the total amount of waste liquid discharged during the concentration process reaching a second preset value, the centrifugal ultrafiltration process is stopped, and the collagen concentrate in the storage tank 20 and the collagen treatment structure 10 is collected.

[0074] Specifically, when the total amount of waste liquid discharged during the concentration process reaches a second preset value, the concentration ends, the centrifugal ultrafiltration process is stopped, and the collagen concentrate remaining in the storage tank 20 and collagen treatment structure 10 can be collected. The second preset value can be set according to the total amount of the initial collagen extract to meet the collagen concentration requirements.

[0075] Further, collecting the collagen concentrate in the storage tank 20 and the collagen processing structure 10 includes: adjusting the pressure in the storage tank 20 to 0.2-0.3 MPa, collecting the collagen concentrate from the first outlet 21 of the storage tank 20, and closing the first outlet 21 after collection; adjusting the speed of the centrifugal processing mechanism 12 to rotate at a speed of 200-300 rpm, and opening the second outlet 13 of the processing chamber 11 to collect the collagen concentrate.

[0076] Specifically, the pressure inside the storage tank 20 is adjusted by opening the compressed air inlet 71 connected to the storage tank 20. The pressure can be adjusted to 0.2-0.3 MPa, for example, 0.2 MPa, 0.22 MPa, 0.24 MPa, 0.26 MPa, 0.28 MPa, 0.3 MPa, etc. Along the direction of gravity, the first discharge port 21 is located below the compressed air inlet 71, allowing the collagen concentrate to be discharged from the first discharge port 21 under the combined action of pressure and gravity, thereby improving the collagen recovery rate. The rotation speed of the centrifugal drive 121 can be adjusted to 200-300 rpm, for example, 200 rpm, 220 rpm, 240 rpm, 260 rpm, 280 rpm, 300 rpm, etc., to reduce collagen residue on the centrifugal ultrafiltration element 122 through centrifugal force, thereby improving the recovery efficiency of the collagen concentrate.

[0077] The collagen processing system 100 of this application embodiment can directly perform concentration processing after the collagen extract has been purified, without the need to change equipment or add reagents, simplifying the operation and making the technology simple. It can also increase the collagen concentration of the concentrated collagen solution; in some embodiments, the collagen concentration can be greater than or equal to 20 mg / ml. It should be noted that the collagen processing system 100 of this application embodiment is designed for collagen with characteristics such as high viscosity, poor water solubility, and easy gelation, to purify and concentrate it, obtaining collagen with a high concentration factor, reaching a concentration of over 20 mg / ml, effectively removing non-collagenous impurities, achieving high yield, and maintaining the complete triple helix structure, meeting the needs of higher concentration collagen medical materials.

[0078] Furthermore, after collecting the collagen concentrate in the storage tank 20 and the collagen treatment structure 10, the process also includes rinsing the collagen treatment structure 10. That is, after collecting the collagen concentrate, the collagen treatment structure 10 is rinsed through the rinsing tank 80 and the rinsing pipeline 90 to extend the service life of the centrifugal ultrafiltration element 122 and reduce contamination.

[0079] Specifically, rinsing the collagen processing structure 10 includes: closing the delivery pipeline 30 between the collagen processing structure 10 and the storage tank 20, and closing the discharge port 111 of the processing chamber 11; connecting the rinsing pipeline 90 to the collagen processing structure 10; adjusting the inlet pressure of the discharge port 111 of the processing chamber 11 to 0.1-0.2 MPa, and adjusting the rotation speed of the centrifugal drive 121 to 200-300 rpm; and performing cleaning.

[0080] Specifically, the delivery pipeline 30 and the discharge port 111 are closed, ensuring that all openings below the return port 113 along the direction of gravity within the collagen treatment structure 10 are closed, allowing the cleaning agent to flow in one direction and improving cleaning efficiency. The cleaning agent can be delivered to the collagen treatment structure 10 through the flushing pipeline 90. To improve delivery efficiency and ensure smooth delivery of the cleaning agent to the collagen treatment structure 10, a flushing pump 73 can be installed to adjust the feed pressure of the treatment chamber 11. The pressure can be adjusted to 0.1MPa-0.2MPa, for example, 0.1MPa, 0.12MPa, 0.14MPa, 0.16MPa, 0.18MPa, 0.2MPa, etc. The rotation speed of the centrifugal drive 121 can be adjusted to 200-300rpm, for example, 200rpm, 220rpm, 240rpm, 260rpm, 280rpm, 300rpm, etc., to ensure sufficient contact between the centrifugal ultrafiltration element 122 and the cleaning agent, increasing the cleaning area and improving cleaning efficiency. Furthermore, the rinsing tank 80 is connected to the cleaning agent injection line 81. The cleaning agent is injected through the cleaning agent injection line 81, and then the cleaning agent is transported to the collagen treatment structure 10, the cleaning treatment chamber 11 and the centrifugal treatment mechanism 12 through the rinsing line 90. Then it is returned to the storage tank 20 through the return line 40, and finally discharged through the first discharge port 21 of the storage tank 20.

[0081] The collagen processing method of this application integrates purification, concentration, and subsequent washing processes into a single system, simplifying operation. The following four tests demonstrate the experimental data performance of the collagen processing structure, system, and method of this application:

[0082] Test 1

[0083] include:

[0084] Step 1: Place the collagen extract into the storage tank 20 of the collagen processing system 100. The concentration of the collagen extract is 2.2 mg / ml, the viscosity is 126 mpa.s, and the material quantity is 50 kg.

[0085] Step 2: The pore size of the centrifugal ultrafiltration element 122 is 10 nm. The parameters of the centrifugal ultrafiltration element 122 during the purification process are set as follows: feed pressure 0.1 MPa, centrifugal ultrafiltration element 122 speed 300 rpm, discharge pressure 0.08 MPa, and continuous replenishment of water for injection is started.

[0086] Step 3: When the conductivity of the collagen purification solution in storage tank 20 is ≤50μS / cm, concentration begins.

[0087] Step 4: Set the parameters of the centrifugal ultrafiltration element 122 for the concentration process as follows: feed pressure 0.2 MPa, centrifugal ultrafiltration element 122 speed 500 rpm, discharge pressure 0.18 MPa, and turn off the water for injection replenishment.

[0088] Step 5: After concentration is complete, open the compressed air inlet 71, set the pressure inside the storage tank 20 to 0.2 MPa, collect the collagen concentrate at the first outlet 21 of the storage tank 20, and after the material in the storage tank 20 has been collected, close the first outlet 21, set the speed of the centrifugal ultrafiltration element 122 to 200 rpm, and open the second outlet 13 of the processing chamber 11 to collect the collagen concentrate.

[0089] Step 6: After collecting the materials, clean them.

[0090] Finally, the purification time was 41 min, the concentration time was 27 min, and 6.44 kg of collagen concentrate was collected. The collagen concentration was 15.7 mg / ml, the viscosity was 18970 mpa.s, the yield was 91.92%, and the concentration factor was 7.14.

[0091] Test 2

[0092] include:

[0093] Step 1: Place the collagen extract into the storage tank 20 of the collagen processing system 100. The concentration of the collagen extract is 3.5 mg / ml, the viscosity is 184 mpa.s, and the material quantity is 42 kg.

[0094] Step 2: The pore size of the centrifugal ultrafiltration element 122 is 30nm. The parameters of the centrifugal ultrafiltration element 122 during the purification process are set as follows: feed pressure 0.05MPa, centrifugal ultrafiltration element 122 speed 500rpm, discharge pressure 0.04MPa, and continuous replenishment of water for injection is started.

[0095] Step 3: When the conductivity of the collagen purification solution in storage tank 20 is ≤50μS / cm, concentration begins.

[0096] Step 4: Set the parameters of the centrifugal ultrafiltration element 122 for the concentration process as follows: feed pressure 0.15MPa, centrifugal ultrafiltration element 122 speed 800rpm, discharge pressure 0.14MPa, and turn off the water for injection replenishment.

[0097] Step 5: After concentration is complete, open the compressed air inlet 71, set the pressure inside the storage tank 20 to 0.15 MPa, collect the collagen concentrate at the first outlet 21 of the storage tank 20, and after the material in the storage tank 20 has been collected, close the first outlet 21, set the speed of the centrifugal ultrafiltration element 122 to 300 rpm, and open the second outlet 13 of the processing chamber 11 to collect the collagen concentrate.

[0098] Step 6: After collecting the materials, clean them.

[0099] Finally, the purification time was 34 min, the concentration time was 21 min, and 6.89 kg of collagen concentrate was collected. The collagen concentration was 18.2 mg / ml, the viscosity was 21550 mpa.s, the yield was 85.30%, and the concentration factor was 5.20.

[0100] Test 3

[0101] include:

[0102] Step 1: Place the collagen extract into the storage tank 20 of the collagen processing system 100. The concentration of the collagen extract is 1.8 mg / ml, the viscosity is 105 mpa.s, and the material quantity is 70 kg.

[0103] Step 2: The pore size of the centrifugal ultrafiltration element 122 is 16nm. The parameters of the centrifugal ultrafiltration element 122 during the purification process are set as follows: feed pressure 0.2MPa, centrifugal ultrafiltration element 122 speed 400rpm, discharge pressure 0.17MPa, and continuous replenishment of water for injection is started.

[0104] Step 3: When the conductivity of the collagen purification solution in storage tank 20 is ≤50μS / cm, concentration begins.

[0105] Step 4: Set the parameters of the centrifugal ultrafiltration element 122 for the concentration process as follows: feed pressure 0.3 MPa, centrifugal ultrafiltration element 122 speed 600 rpm, discharge pressure 0.29 MPa, and turn off the water for injection replenishment.

[0106] Step 5: After concentration is complete, open the compressed air inlet 71 and set the pressure to 0.2 MPa. Collect the collagen concentrate at the first outlet 21 of the storage tank 20. After the material in the storage tank 20 has been collected, close the first outlet 21, set the centrifugal ultrafiltration element 122 speed to 250 rpm, and open the second outlet 13 of the processing chamber 11 to collect the collagen concentrate.

[0107] Step 6: After collecting the materials, clean them.

[0108] Finally, the purification time was 28 minutes, the concentration time was 37 minutes, and 5.16 kg of collagen concentrate was collected. The collagen concentration was 21.7 mg / ml, the viscosity was 26470 mpa.s, the yield was 88.87%, and the concentration factor was 12.06.

[0109] Test 4

[0110] include:

[0111] Step 1: Place the collagen extract into the storage tank 20 of the collagen processing system 100. The concentration of the collagen extract is 4.1 mg / ml, the viscosity is 230 mpa.s, and the material quantity is 35 kg.

[0112] Step 2: The pore size of the centrifugal ultrafiltration element 122 is 24nm. The parameters of the centrifugal ultrafiltration element 122 during the purification process are set as follows: feed pressure 0.3MPa, centrifugal ultrafiltration element 122 speed 200rpm, discharge pressure 0.27MPa, and continuous replenishment of water for injection is started.

[0113] Step 3: When the conductivity of the collagen purification solution in storage tank 20 is ≤50μS / cm, concentration begins.

[0114] Step 4: Set the parameters of the centrifugal ultrafiltration element 122 for the concentration process as follows: feed pressure 0.5 MPa, centrifugal ultrafiltration element 122 speed 700 rpm, discharge pressure 0.48 MPa, and turn off the water for injection replenishment.

[0115] Step 5: After concentration is complete, open the compressed air inlet 71 and set the pressure to 0.25 MPa. Collect the collagen concentrate at the first outlet 21 of the storage tank 20. After the material in the storage tank 20 has been collected, close the first outlet 21, set the centrifugal ultrafiltration element 122 speed to 280 rpm, and open the second outlet 13 of the processing chamber 11 to collect the collagen concentrate.

[0116] Step 6: After collecting the materials, clean them.

[0117] Finally, the purification time was 47 minutes, the concentration time was 33 minutes, and 5.46 kg of collagen concentrate was collected. The collagen concentration was 23.1 mg / ml, the viscosity was 32280 mPa·s, the yield was 87.89%, and the concentration factor was 5.63.

[0118] As can be seen from the above data, the collagen processing structure, system and method of this application embodiment are simple to operate and technically simple. After purification and concentration, the collagen extract has a high collagen concentration, a short processing time, improved ultrafiltration efficiency, and improved collagen recovery rate.

[0119] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application, and they should all be covered within the scope of the claims and specification of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the embodiments can be combined in any way. This application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. A collagen processing structure, characterized in that, It includes a processing chamber (11), which is provided with a discharge port (111); A centrifugal processing mechanism (12) includes a centrifugal drive (121) and a centrifugal ultrafiltration element (122). The centrifugal ultrafiltration element (122) is connected to the centrifugal drive (121) and rotates under the centrifugal drive (121). The centrifugal ultrafiltration element (122) is disposed in the processing chamber (11), and ultrafiltration membrane layers (1221) are provided on opposite sides of the centrifugal ultrafiltration element (122); the centrifugal ultrafiltration element (122) is provided with a filtration chamber (1222) inside, and the centrifugal ultrafiltration element (122) is also provided with a waste liquid hole (1223) communicating with the filtration chamber (1222), and the waste liquid hole (1223) is communicating with the discharge port (111).

2. The collagen treatment structure according to claim 1, characterized in that, The centrifugal drive (121) includes a hollow centrifugal shaft (1211) extending into the processing chamber (11). The centrifugal shaft (1211) is provided with a hollow channel (1212), and one end away from the centrifugal drive (121) is connected to the discharge port (111). Each of the centrifugal ultrafiltration elements (122) is provided with a mounting hole (1224), and the centrifugal shaft (1211) is fixed in the mounting hole (1224); The waste liquid hole (1223) penetrates the inner wall of the mounting hole (1224); The centrifugal shaft (1211) is provided with a liquid inlet, which is connected to the waste liquid hole (1223).

3. The collagen treatment structure according to claim 2, characterized in that, Each of the mounting holes (1224) is located at the center of the corresponding centrifugal ultrafiltration element (122).

4. The collagen treatment structure according to claim 3, characterized in that, Each of the centrifugal ultrafiltration elements (122) is disc-shaped, the ultrafiltration membrane layer (1221) is located on opposite sides of the disc, and the mounting hole (1224) is the center of the disc.

5. The collagen treatment structure according to claim 4, characterized in that, The processing chamber (11) also includes a feed inlet (112) and a return outlet (113). Along the direction of gravity, the discharge outlet (111) and the feed inlet (112) are both located below the return outlet (113).

6. The collagen treatment structure according to claim 1, characterized in that, The pore size of each of the ultrafiltration membrane layers (1221) ranges from 10 nm to 30 nm.

7. A collagen processing system, characterized in that, It includes the collagen-treated structure (10) according to any one of claims 1-6; The collagen processing system also includes a storage tank (20), a delivery pipeline (30), a return pipeline (40), a sewage pipeline (50), and a water injection pipeline (60); The delivery pipeline (30) connects the storage tank (20) and the collagen processing structure (10); The reflux pipeline (40) connects the collagen processing structure (10) and the storage tank (20); The sewage pipe (50) is connected to the discharge port (111); The water injection pipeline (60) is connected to the storage tank (20).

8. The collagen processing system according to claim 7, characterized in that, The water injection pipeline (60) can be controlled to operate in two states: water injection off or water injection into the storage tank (20). When it is in the water injection into the storage tank (20) state, the amount of water injected into the storage tank (20) is equal to the amount of waste liquid discharged from the discharge port (111).

9. The collagen processing system according to claim 7, characterized in that, It also includes a pressure regulating assembly (70), which includes a compressed air inlet (71) and an ultrafiltration pump (72); The compressed air inlet (71) is connected to the storage tank (20) and is used to regulate the pressure inside the storage tank (20); The ultrafiltration pump (72) is connected at one end to the storage tank (20) and at the other end to the collagen processing structure (10).

10. The collagen processing system according to claim 7, characterized in that, It also includes a rinsing tank (80), a rinsing pump (73), and a rinsing pipeline (90), the rinsing pipeline (90) connecting the collagen treatment structure (10) and the rinsing tank (80).

11. A method for processing collagen, characterized in that, include: The initial collagen extract in the storage tank (20) is transported to the collagen processing structure (10); The initial collagen extract is purified by cyclic centrifugation and ultrafiltration through the centrifugation mechanism (12) within the collagen processing structure (10); In response to the conductivity within the storage tank (20) meeting a first preset value, the purification of the initial collagen extract is stopped; wherein, The purification of the initial collagen extract by cyclic centrifugation and ultrafiltration through the centrifugation mechanism (12) within the collagen processing structure (10) includes: Discharge the waste liquid generated in each centrifugal ultrafiltration process; return the collagen liquid extracted in each centrifugal ultrafiltration process to the storage tank (20), and at the same time replenish the storage tank (20) with the same amount of water as the waste liquid; The mixture of the remaining initial collagen extract in the storage tank (20), the supplemented water, and the collagen solution extracted by the centrifugal ultrafiltration process is transported back to the collagen processing structure (10) for another centrifugal ultrafiltration process, and the process is circulated until the initial collagen extract is no longer purified.

12. The method for processing collagen according to claim 11, characterized in that, After stopping the purification of the initial collagen extract, the method further includes concentrating the purified initial collagen extract, including: The purified initial collagen extract in the storage tank (20) is continued to be transported to the collagen processing structure (10); The centrifugal processing mechanism (12) within the collagen processing structure (10) continues to perform cyclic centrifugal ultrafiltration to concentrate and purify the initial collagen extract. During the process of cyclic centrifugal ultrafiltration to concentrate and purify the initial collagen extract, water is stopped from being added to the storage tank (20), and the waste liquid generated in each centrifugal ultrafiltration is discharged. The collagen liquid extracted in each centrifugal ultrafiltration is returned to the storage tank (20). The mixture formed by the remaining purified initial collagen extract and the collagen liquid extracted in the centrifugal ultrafiltration in the storage tank (20) is transported back to the collagen processing structure (10) for centrifugal ultrafiltration again, and the process is repeated until the centrifugal ultrafiltration is stopped. When the total amount of waste liquid discharged from the concentration process reaches a second preset value, the centrifugal ultrafiltration process is stopped, and the collagen concentrate in the storage tank (20) and collagen treatment structure (10) is collected.

13. The method for processing collagen according to claim 11 or 12, characterized in that, During the process of purifying the initial collagen extract by circulating centrifugation and ultrafiltration in the collagen processing unit (10), the rotation speed of the centrifugation unit (12) is 100-500 rpm, the feed pressure of the processing chamber (11) is 0.1-0.3 MPa, and the discharge pressure of the processing chamber (11) is 0.01-0.3 MPa.

14. The method for processing collagen according to claim 11 or 12, characterized in that, During the concentration process of the purified initial collagen extract, the centrifuge (12) rotates at 500-800 rpm, the feed pressure of the processing chamber (11) is 0.1-0.5 MPa, and the discharge pressure of the processing chamber (11) is 0.05-0.5 MPa.

15. The method for processing collagen according to claim 11, characterized in that, The collection of collagen concentrate within the storage tank (20) and collagen processing structure (10) includes: Adjust the pressure inside the storage tank (20) to 0.2-0.3 MPa, collect the collagen concentrate from the first outlet (21) of the storage tank (20), and close the first outlet (21) after collection is completed; Adjust the centrifugal processing mechanism (12) to rotate at a speed of 200-300 rpm, and open the second discharge port (13) of the processing chamber (11) to collect the collagen concentrate.

16. The method for processing collagen according to claim 11, characterized in that, After collecting the collagen concentrate from the storage tank (20) and the collagen treatment structure (10), the process further includes rinsing the collagen treatment structure (10), including: Close the delivery pipeline (30) between the collagen processing structure (10) and the storage tank (20), and close the drain pipeline (50); Connect the flushing pipe (90) to the collagen treatment structure (10); Turn on the flushing pump on the flushing pipeline, adjust the inlet pressure of the discharge port (111) of the treatment chamber (11) to 0.1-0.2MPa, and adjust the speed of the centrifugal treatment mechanism (12) to 200-300rpm for cleaning.

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