Light-initiated chemiluminescence detection apparatus and detection method

Abstract

The present application relates to a light-initiated chemiluminescence detection apparatus and detection method. The detection apparatus comprises: a sample feeding module; a reagent module; a pipetting mechanism comprising a first driving assembly and a pipettor, wherein the pipettor can be driven by the first driving assembly to move in a three-dimensional space and can be connected to disposable pipette tips, so as to transfer a sample and a first reagent; a cup grasping mechanism comprising a second driving assembly and a cup grasping assembly, wherein the cup grasping assembly can be used for grasping reaction cups, and is driven by the second driving assembly to perform positional dispatching on the grasped reaction cups; an incubation mechanism, which comprises a rotary disk configured to circumferentially load a plurality of reaction cups, and incubates sample-reagent mixtures in the reaction cups; a detection mechanism, which is arranged on the incubation mechanism and is configured to perform light initiation and detection on the incubated sample-reagent mixtures; and a liquid-filling assembly, which completes at a specific position the filling of a second reagent into the reaction cups. The solution provided in the present application can omit a washing procedure in chemiluminescence analysis and detection, thereby improving the detection efficiency.

Description

Photo-induced chemiluminescence detection equipment and detection methods

[0001] This application claims priority to Chinese Patent Application No. 2024104965939, filed on April 23, 2024, entitled "Photostimulated Chemiluminescence Detection Device and Detection Method", the entire contents of which are incorporated herein by reference. Technical Field

[0002] This application relates to the field of photo-induced chemiluminescence immunoassay technology, and in particular to a photo-induced chemiluminescence detection device and detection method. Background Technology

[0003] In chemiluminescence analysis, regardless of the chemiluminescence principle, commercially available detection equipment often includes a cleaning process. For example, patent application number CN201810117325.6 discloses a fully automated photo-induced chemiluminescence detector. This patent clearly states that the sample addition mechanism is the left arm of the sample addition module 4, and the reagent addition mechanism is the right arm of the sample addition module 4. After the left arm of the sample addition arm is filled with the solution containing the sample to be tested, it can be cleaned through the first needle washing tank in the needle washing tank 81. After the right arm of the sample addition arm is filled with the reaction reagent, it can be cleaned through the second needle washing tank in the needle washing tank 81.

[0004] Obviously, in the relevant technology, the sample dispensing arm adopts a left arm and a right arm depending on the sample being dispensed. Compared with using the same sample dispensing arm for sample dispensing, the cleaning time is shortened. However, it is undeniable that the existence of the cleaning process undoubtedly increases the detection cycle in the detection and analysis, thereby reducing the detection efficiency.

[0005] Summary of the Invention

[0006] To address or partially address the problems existing in related technologies, this application provides a photo-induced chemiluminescence detection device and detection method, which can eliminate the cleaning process in chemiluminescence analysis and detection, thereby improving detection efficiency.

[0007] The first aspect of this application provides a photo-induced chemiluminescence detection device, comprising:

[0008] The sample introduction module is used to store at least a sample tube holder and a disposable pipette tip, and the sample tube contains a sample.

[0009] The reagent module is used to store the first and second reagents;

[0010] The pipetting mechanism includes a first driving component and a pipette. The pipette is mounted on the first driving component and can move in three-dimensional space under the drive of the first driving component. The pipette can be connected to a disposable pipette tip to realize the aspiration and transfer of sample and first reagent.

[0011] The cup-grabbing mechanism includes a second drive component and a cup-grabbing component, the cup-grabbing component being mounted on the second drive component; the cup-grabbing component can be used to grab a reaction cup and perform position scheduling of the grabbed reaction cup under the drive of the second drive component;

[0012] An incubation apparatus includes at least one turntable for loading a plurality of reaction cups along its circumference; the incubation apparatus incubates a mixture of reagents in the reaction cups;

[0013] The testing facility is located on the incubation facility and is used to photoexcite the incubated mixed reagent and detect the chemiluminescence produced by the mixed reagent after photoexcitation.

[0014] And a liquid injection assembly, which can complete the injection of a second reagent into the reaction cup inside the incubation mechanism at a specific location.

[0015] A second aspect of this application provides a photo-induced chemiluminescence detection method, which is based on the photo-induced chemiluminescence detection device of the first aspect, comprising:

[0016] The first drive component in the pipetting mechanism controls the pipette to connect to the disposable pipette tip. Driven by the first drive component, the disposable pipette tip transfers and aspirates the sample and the first reagent into the corresponding reaction cup to obtain a mixed reagent. After transfer, the pipette discards the aspirated disposable pipette tip.

[0017] According to the process requirements, the cup-grabbing component in the cup-grabbing mechanism, driven by the second drive component, positions the grabbing reaction cup containing the mixed reagent, so that the incubation mechanism incubates the mixed reagent in the reaction cup; wherein, for secondary incubation, after the first incubation, the liquid injection component can complete the injection of the second reagent into the reaction cup inside the incubation mechanism at a specific position for the second incubation.

[0018] The testing agency performs photoexcitation on the incubated mixed reagent and detects the chemiluminescence produced by the mixed reagent after photoexcitation;

[0019] The cup-grabbing mechanism performs a cup-throwing process on the corresponding reaction cups after detecting the mixed reagents.

[0020] The technical solution provided in this application may include the following beneficial effects:

[0021] The technical solution of this application is applied to photoluminescence analysis and detection. The pipette, using disposable pipette tips, can transfer samples or first reagents into reaction cups. After transferring the sample or reagent, the pipetting mechanism can control the pipette to discard the corresponding disposable pipette tip, eliminating the need for cleaning the conventional sample or reagent aspiration mechanism, saving analysis and detection time, shortening the detection cycle, and improving detection efficiency. This application also features a specially designed cup-grabbing mechanism to manage the position of the reaction cups. This mechanism can cooperate with the turntable in the incubation mechanism. Compared to the incubation mechanism where the movement of reaction cups is entirely within the incubation mechanism, the internal complexity of the incubation mechanism is reduced, thereby improving the effective utilization rate of the incubation mechanism. This allows for a relatively smaller incubation mechanism volume while maintaining a consistent incubation environment, improving incubation quality, and ultimately enhancing the quality of the detection results.

[0022] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Attached Figure Description

[0023] The above and other objects, features and advantages of this application will become more apparent from the following description of exemplary embodiments of this application in conjunction with the accompanying drawings, wherein the same reference numerals generally represent the same components.

[0024] Figure 1 is a perspective view of the photo-induced chemiluminescence detection device shown in an embodiment of this application;

[0025] Figure 2 is a perspective view of the pipetting mechanism shown in an embodiment of this application;

[0026] Figure 3 is a perspective view of the cup-gripping mechanism shown in an embodiment of this application;

[0027] Figure 4 is a perspective view of the incubation facility shown in an embodiment of this application;

[0028] Figure 5 is a schematic diagram of the piping connection of the injection assembly shown in an embodiment of this application;

[0029] Figure 6 is a schematic diagram of the liquid injection assembly shown in an embodiment of this application;

[0030] Figure 7 is a schematic diagram of the structure of the injection assembly and pipette shown in the embodiment of this application;

[0031] Figure 8 is a schematic diagram of the temperature control component shown in an embodiment of this application;

[0032] Figure 9 is a schematic diagram of the structure of the second positioning component fixing the reaction cup in the incubation mechanism shown in an embodiment of this application;

[0033] Figure 10 is a bottom view of the structure of the incubation mechanism shown in an embodiment of this application;

[0034] Figure 11 is a schematic diagram of the gripper assembly when it grips the reaction cup, as shown in an embodiment of this application;

[0035] Figure 12 is a front view of the gripper assembly in an embodiment of this application when it grips the reaction cup;

[0036] Figure 13 is a left view of the gripper assembly in an embodiment of this application when it grips the reaction cup;

[0037] Figure 14 is a top view of the gripper assembly at the cam when it grips the reaction cup, as shown in an embodiment of this application.

[0038] Figure 15 is a schematic diagram of the gripper assembly in an embodiment of this application when the gripper is opened to its maximum extent;

[0039] Figure 16 is a top view of the gripper assembly at the cam when the gripper is opened to its maximum extent, as shown in an embodiment of this application.

[0040] Figure 17 is another structural schematic diagram of the gripper assembly shown in an embodiment of this application;

[0041] Figure 18 is a top view of the cam structure of the gripper assembly shown in Figure 17 when it fails to grip the reaction cup;

[0042] Figure 19 is a schematic diagram of the sealing mechanism shown in an embodiment of this application;

[0043] Figure 20 is an exploded view of the sealing mechanism shown in an embodiment of this application;

[0044] Figure 21 is a top view schematic diagram of the sealing mechanism shown in an embodiment of this application;

[0045] Figure 22 is another top view schematic diagram of the sealing mechanism shown in an embodiment of this application;

[0046] Figure 23 is an exploded view of the sealing mechanism shown in an embodiment of this application (the third drive component is hidden);

[0047] Figure 24 is a schematic diagram of the external structure of the heat dissipation mechanism shown in an embodiment of this application;

[0048] Figure 25 is a cross-sectional view along the AA direction in Figure 24;

[0049] Figure 26 is a cross-sectional view along the BB direction in Figure 24;

[0050] Figure 27 is the right view of Figure 24;

[0051] In the diagram: 1. Frame; 100. Reaction cup; 101. Cup feeding mechanism; 102. Cup feeding chamber; 103. Cup feeding channel; 104. Cup pushing mechanism; 105. Waste box; 3. Reagent module; 300. Reagent chamber; 301. First reagent chamber; 302. Liquid outlet; 303. Sealing mechanism; 304. Cover plate; 305. First through hole; 306. Third drive assembly; 307. Position sensor; 308. Heating plate; 309. Second through hole; 310. Insulation plate; 311. Third through hole; 312. Temperature sensor; 313. Over-temperature protector; 314. Guide rail; 315. Heat dissipation mechanism; 316. Semiconductor cooling unit; 317. Heat dissipation shell; 318. Air inlet; 319. Air outlet; 310. 9. Receiving cavity; 320. Heat dissipation duct; 321. Opening; 322. Semiconductor cooling chip; 323. Heat dissipation fin assembly; 4. Pipetting mechanism; 400. Pipette; 401. First drive assembly; 5. Cup gripping mechanism; 500. Cup gripping assembly; 501. Fixing plate; 502. Spring fixing rod; 503. Rotating shaft; 504. Gripper; 505. Left gripper; 506. Right gripper; 507. Left roller; 508. Right roller; 509. Gripper clamping spring; 510. Cam; 511. Motor; 512. Gripper center positioning spring; 513. Gripper opening sensor; 514. Cupless sensor; 515. Cupless sensor baffle; 516. Gripper opening sensor baffle; 517. Second drive assembly; 51 8. Horizontal drive component; 519. Vertical drive component; 520. Drive motor; 521. Second pulley; 522. Belt; 523. Vertical slide rail; 524. Horizontal step loss detection component; 525. First encoder; 526. First optocoupler; 527. Vertical step loss detection component; 528. Second encoder; 529. Second optocoupler; 530. Zero-position optocoupler; 6. Incubation mechanism; 600. Turntable; 601. Incubation tray; 602. Measuring disc; 603. Incubation shell; 604. Incubation shell cover; 605. Incubation chamber; 606. Second positioning component; 607. Fixed block; 608. Movable block; 609. Incubation reset detection unit; 610. Incubation step counting unit; 611. Measuring reset detection unit; 61 2. Photometric and pedometer unit; 613. Socket; 614. Heating component; 615. Limiting block; 616. Elastic element; 7. Detection mechanism; 701. Photoexcitation component; 702. Detection component; 703. Excitation position; 704. Detection position; 8. Liquid injection component; 801. Liquid storage section; 802. Injector; 803. Liquid injection base; 804. Inlet pipe; 805. Outlet pipe; 806. Injection end; 807. First switching component; 808. Second switching component; 809. Container; 810. Substrate container; 811. Cleaning solution container; 812. Injection position; 813. Waste discharge position; 814. Base; 815. Connecting part; 816. Drive hole; 817. Temperature control component; 818. Housing;819. Heating element; 820. Piping channel; 821. Piping fastener; 822. Limiting hole; 823. First positioning component; 824. Contact element; 825. First blocking element; 826. Second blocking element; 827. Slide rail. Detailed Implementation

[0052] Embodiments of this application will now be described in more detail with reference to the accompanying drawings. While embodiments of this application are shown in the drawings, it should be understood that this application may be implemented in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided to make this application more thorough and complete, and to fully convey the scope of this application to those skilled in the art.

[0053] In related technologies, chemiluminescence analysis and detection equipment often involves a cleaning process, resulting in a long detection cycle and low detection efficiency. To address these issues, this application provides a photo-induced chemiluminescence detection device that effectively improves sample detection efficiency and quality. Photo-induced chemiluminescence immunoassay, as a type of chemiluminescence immunoassay, belongs to a homogeneous reaction mode, unlike heterogeneous reaction modes, thus eliminating the need for cleaning and separating unbound samples and reagents.

[0054] As shown in Figures 1 to 4, this application provides a photo-induced chemiluminescence detection device, including a sample introduction module, a reagent module 3, a pipetting mechanism 4, a cup gripping mechanism 5, an incubation mechanism 6, a detection mechanism 7, and a liquid injection assembly 8. The sample introduction module is used to store at least a sample tube holder and a disposable pipette tip, and the sample tube contains a sample. The reagent module 3 is used to store a first reagent and a second reagent. The pipetting mechanism 4 includes a first driving assembly 401 and a pipette 400. The pipette 400 is mounted on the first driving assembly 401 and can move in three-dimensional space under the drive of the first driving assembly 401. The pipette 400 can be connected to a disposable pipette tip to realize the aspiration and transfer of the sample and the first reagent. The cup gripping mechanism 5 includes a second driving assembly 51. 7 and a cup gripping assembly 500, which is mounted on the second drive assembly 517; the cup gripping assembly 500 can be used to grip the reaction cup 100 and perform position scheduling of the gripped reaction cup 100 under the drive of the second drive assembly 517; the incubation mechanism 6 includes at least one turntable 600, which is used to load a plurality of reaction cups 100 along its circumference; the incubation mechanism 6 incubates the mixed reagent in the reaction cup 100; the detection mechanism 7 is disposed on the incubation mechanism 6 and is used to photoexcite the incubated mixed reagent and detect the chemiluminescence generated by the mixed reagent after photoexcitation; and a liquid injection assembly 8, which can complete the injection of a second reagent into the reaction cup 100 inside the incubation mechanism 6 at a specific position.

[0055] In this embodiment, the photochemiluminescence detection device can be a single unit. The incubation unit 6, the pipetting unit 4, and the cup-gripping unit 5 are all mounted on the frame 1, with the pipetting unit 4 and the cup-gripping unit 5 located above the incubation unit 6. The sample injection module and the reagent module 3 are positioned at appropriate locations on the frame 1. The sample injection module can be used to store the sample tube holder and disposable pipette tips. In this embodiment, the disposable pipette tips can also be referred to as tip heads. The sample tube holder is used to store sample tubes containing samples. The reagent module 3 can be used to store reagents, such as the first reagent and the second reagent. In photo-induced chemiluminescence detection, an empty reaction cup 100 is loaded onto the sample dispensing position on the turntable 600 of the incubation mechanism 6. The first driving component 401 in the pipetting mechanism 4 drives the pipette 400 to the injection module for attaching a tip and taking a sample through the tip. The pipette 400 then moves back to the sample dispensing position to add the sample into the reaction cup 100. After sample dispensing, the aspirated tip is discarded. The pipette 400 moves to the injection module to attach a tip and draws the first reagent from the reagent module 3 through the tip, adding the first reagent into the aforementioned reaction cup 100. The tip can be discarded or removed as needed. The Tip reuse module is temporarily used; and, according to process requirements, the cup gripping component 500 in the cup gripping mechanism 5, driven by the second drive component 517, performs position scheduling on the gripped reaction cup 100, so that the incubation mechanism 6 incubates the mixed reagent in the reaction cup 100; wherein, for secondary incubation, after the first incubation, the liquid injection component 8 can complete the injection of the required second reagent into the reaction cup 100 inside the incubation mechanism 6 at a specific position for the second incubation; the detection mechanism 7 performs photoexcitation on the incubated mixed reagent and detects the chemiluminescence generated by the mixed reagent after photoexcitation.

[0056] In one embodiment, as shown in Figures 5 to 8, the liquid injection assembly 8 includes a liquid storage section 801, a liquid injector 802, a liquid injection base 803, an inlet pipe 804, and an outlet pipe 805. The liquid storage section 801 is located in the reagent module 3 and is used to store at least the second reagent. The inlet pipe 804 connects the inlet of the liquid injector 802 to the liquid storage section 801, and the outlet pipe 805 connects to the outlet of the liquid injector 802 and is partially coiled within the liquid injection base 803. The liquid injection base 803 is movably mounted on the incubation mechanism 6 and is detachably connected to the pipette 400. When the liquid injection base 803 is connected to the pipette 400, the pipette 400, driven by the first driving assembly 401, moves the injection end 806 of the outlet pipe 805 to the corresponding injection position 812, so that the second reagent can be injected into the corresponding reaction cup 100 in the incubation mechanism 6 through the liquid injector 802.

[0057] In one embodiment, as shown in FIG5, the liquid injection assembly 8 further includes a first switching component 807. The first switching component 807 includes two first stationary ends and one first moving end. The first moving end is connected to the liquid injector 802, and the two first stationary ends are respectively connected to the liquid inlet pipe 804 and the liquid outlet pipe 805. The first switching component 807 can be used to control the connection state between the liquid injector 802 and the liquid inlet pipe 804 and the liquid outlet pipe 805. The liquid storage section 801 includes N+1 containers 809. The liquid injection assembly 8 further includes N second switching components 808, where N is a positive integer. The second switching component 808 includes two second stationary ends and one second moving end. The N second switching components 808 are connected in series, and the remaining N+1 second stationary ends are each connected to one container 809. The remaining second moving end is connected to the liquid injector 802 through the liquid inlet pipe 804.

[0058] In one embodiment, as shown in FIG7, the liquid injection assembly 8 further includes a waste discharge position 813. When the liquid injection end 806 is at the waste discharge position 813, the liquid injection assembly 8 is used to discharge the cleaning reagent in the cleaning reagent container 809 in the container 809 through the liquid outlet pipe 805. When the liquid injection end 806 is at the liquid injection position 812, the liquid injection assembly 8 is used to inject the second reagent from the substrate container 810 into the reaction cup 100.

[0059] In this embodiment, taking three containers 809 as an example, there are two substrate containers 810 and one cleaning reagent container 809. The two substrate containers 810 contain substrate A and substrate B respectively, and the cleaning reagent container 809 contains cleaning solution. Correspondingly, there are two second switching components 808. The two second stationary ends of one second switching component 808 are each connected to one substrate container 810, and one second moving end is connected to one second stationary end of the other second switching component 808. The other second stationary end of the other second switching component 808 is connected to the cleaning reagent container 809. One second moving end is connected to the first stationary end of the first switching component 807 through the liquid inlet pipe 804. The other first stationary end of the first switching component 807 is connected to the liquid injection base 803 through the liquid outlet pipe 805, and the first moving end is connected to the liquid injector 802. The second switching component 808 can be used to control the connection state between different containers 809 and the injector 802 so that different solutions are input into the injector 802. The first switching component 807 can be used to control the connection state between the injector 802 and the outlet pipeline 805. The first switching component 807 and the second switching component 808 can be three-way valves.

[0060] During injection, the second switching component 808 connects one of the two substrate containers 810 to the inlet pipe 804, and the first switching component 807 connects the inlet pipe 804 to the injector 802, so that the substrate solution in the substrate container 810 is input into the injector 802. Then, the first switching component 807 switches the path so that the injector 802 is connected to the outlet pipe 805, so that the substrate solution in the injector 802 is output to the outlet pipe 805. The outlet pipe 805 outputs the substrate in it to the reaction cup 100 through the injection base 803 to participate in the chemoimmunoassay.

[0061] When cleaning the inlet pipe 804 and outlet pipe 805, the corresponding second switching component 808 is controlled to connect the cleaning solution container 811 and the inlet pipe 804, and the corresponding passage of the first switching component 807 controls the injector 802 to connect with the cleaning solution container 811, so that the cleaning solution in the cleaning solution container 811 is input into the injector 802 to clean the inlet pipe 804; then the passage is switched by the first switching component 807 to connect the injector 802 with the outlet pipe 805, so that the cleaning solution in the injector 802 is output to the outlet pipe 805 to clean the outlet pipe 805. After cleaning, the waste liquid is discharged through the injection base 803, completing the cleaning of the pipe.

[0062] This embodiment of the application achieves liquid injection and pipeline cleaning through a single inlet pipe 804 and outlet pipe 805. It can also prevent interference between the injection and cleaning solutions, and achieve continuous injection and cleaning, which greatly saves space and reduces costs. Furthermore, by using the outlet pipe 805 (soft tube) instead of the injection needle for liquid injection, the step of cleaning the inner and outer walls of the injection needle is eliminated. When the cleaning solution passes through the outlet pipe 805 and is discharged, the entire outlet pipe 805, including the injection port, is cleaned, preventing pipeline crystallization and contamination. Even if a blockage occurs, the outlet blockage problem can be solved by trimming the outlet pipe 805.

[0063] As shown in Figures 6 and 7, the injection base 803 includes a base 814, which is slidably mounted on the top of the incubation mechanism 6. The base 814 has a connecting portion 815 adapted to the pipette 400, and the connecting portion 815 has a driving hole 816. The injection assembly 8 also includes an injection position 812 and a waste discharge position 813. The top of the pipette 400 is inserted into the driving hole 816 under the drive of the first driving assembly 401, allowing the first driving assembly 401 to drive the base 814 to reciprocate between the injection position 812 and the waste discharge position 813. The base 814 can reciprocate via a slide rail provided on the incubation mechanism 6, allowing the injection base 803 to move smoothly between the injection position 812 and the waste discharge position 813.

[0064] In one embodiment, as shown in FIG8, the liquid injection assembly 8 further includes a temperature control assembly 817 disposed on the liquid injection base 803. The temperature control assembly includes a housing 818 and a heating element 819 disposed in the housing 818. The housing 818 is disposed on one side of the liquid injection base 803, and the inner side of the housing 818 is provided with a pipeline channel 820 for accommodating the liquid outlet pipe 805. The liquid injection end 806 of the liquid outlet pipe 805 extends from the pipeline channel 820 to the outside of the housing 818. The pipeline channel 820 can be a zigzag groove or a disc-shaped groove, as long as it can facilitate pipe passage. The shape of the pipeline channel 820 is not limited. The temperature control assembly 817 is disposed on the liquid outlet pipe 805 and can be used to control the temperature of the solution in the liquid outlet pipe 805 to ensure the temperature of the substrate solution injected into the reaction vessel 100. In photocatalytic chemiluminescence immunoassay, the recommended transport and storage temperature for the substrate solution is 2–8°C. The substrate solution needs to be brought back to room temperature before use. If the substrate solution temperature does not meet the requirements, it will affect the detection results of the immunoassay analyzer. This embodiment uses a temperature control component 817 to heat the substrate solution in the dispensing line 805, ensuring the substrate solution's operating temperature, reducing its impact on the detection results, and guaranteeing the reliability and stability of the dispensing temperature.

[0065] In one embodiment, as shown in Figures 6 and 7, the temperature control component 817 further includes a pipe fixing member 821. The pipe fixing member 821 is disposed on the base 814, and a limiting hole 822 is formed in the vertical direction within the pipe fixing member 821. The limiting hole 822 is matched with the liquid injection end 806 to limit the liquid injection so that the liquid injection end 806 is injected vertically. Considering that the liquid outlet pipe 805 in this embodiment is a flexible tube, in order to ensure the perpendicularity of the liquid injection end 806 to the reaction cup 100 during liquid injection, the liquid injection end 806 can be fixed and limited by the pipe fixing member 821. The limiting hole 822 is formed in the vertical direction within the pipe fixing member 821, and the liquid injection end 806 is inserted into the limiting hole 822 to maintain a vertical state.

[0066] In this embodiment, the pipette 400 extends into the drive hole 816 of the connecting part 815, driving the injection base 803, which is fixedly connected to the seat 814, to reciprocate. This causes the injection end 806 to move between the injection position 812 and the waste discharge position 813. This eliminates the need to replace the reaction cup 100 and waste container at the same location; only the reaction cup 100 needs to be placed at the position corresponding to the injection position 812, and the waste container at the waste discharge position 813. This reduces the overall control cost of the immunoassay analyzer and makes the injection and tubing cleaning operations simpler and more efficient. The component driving the reciprocating movement of the injection base 803 in the injection assembly 8 is the pipette 400 in the pipetting mechanism 4, which allows the pipette 400 to have dual functions and further reduces costs.

[0067] In one embodiment, as shown in FIG7, the injection assembly 8 further includes a first positioning assembly 823, which is used to detect whether the injection end 806 has moved to the injection position 812 or the waste discharge position 813. The first positioning assembly 823 can be connected to the main controller. The main controller receives the detection result of the first positioning assembly 823 and determines whether the injection end 806 has moved to the injection position 812 or the waste discharge position 813. If the injection end 806 moves to the injection position 812 or the waste discharge position 813, the main controller controls the pipette 400 to stop moving.

[0068] Referring to Figure 7, the first positioning component 823 includes a contact member 824 disposed on the base 814, and a first stop member 825 and a second stop member 826 respectively disposed near the injection position 812 and the waste discharge position 813. The contact member 824 and the first stop member 825 cooperate to limit the movement of the injection end 806 to the injection position 812, and the contact member 824 and the second stop member 826 cooperate to limit the movement of the injection end 806 to the waste discharge position 813. In order not to affect the installation of the reaction cup 100 and the waste liquid box at the injection position 812 and the waste discharge position 813, the first stop member 825 and the second stop member 826 are disposed near the injection position 812 and the waste discharge position 813, but at a certain distance from them. When the contact element 824 contacts the first stop element 825, the injection end 806 reaches the injection position 812. The main controller receives the contact signal and controls the pipette 400 to stop moving, and the injection end 806 injects the substrate solution. When the contact element 824 contacts the second stop element 826, the injection end 806 reaches the waste discharge position 813. The main controller receives the contact signal and controls the pipette 400 to stop moving, and the injection end 806 injects the cleaning solution. The contact element 824 can be a magnet, and the first stop element 825 and the second stop element 826 can be metal microswitches. When the magnet approaches the metal microswitch and is attracted, the metal microswitch generates a micro-motion signal. The main controller receives the micro-motion signal and controls the pipette 400 to stop moving.

[0069] In one embodiment, as shown in Figure 4, the number of turntables 600 is M, with at least M-1 turntables 600 arranged in a ring shape, and all turntables 600 are coaxially nested, where M is a positive integer greater than 1. The rotation drive of each turntable 600 is independent, where M is a positive integer greater than 1. If M = 2, then the two turntables 600 are an incubation disc 601 and a measuring disc 602, which are coaxially nested and rotate independently around their own axes. Each turntable 600 includes an inner disc and a ring-shaped outer disc, with the outer disc nested outside the inner disc. The outer and inner discs are driven by different driving mechanisms, allowing them to rotate at different times. The inner disc is the measuring disc 602, and the outer disc is the incubation disc 601. The coaxial inner and outer discs also reduce the spatial arrangement of the incubation mechanism 6. Compared to two separate turntables 600, the inner and outer discs in this embodiment are significantly more space-efficient, which is beneficial for the miniaturization of the testing equipment.

[0070] In one embodiment, as shown in FIG4, the detection mechanism 7 includes a photoexcitation component 701 and a detection component 702 disposed on the incubation mechanism 6. The photoexcitation component 701 and the detection component 702 are located on the same rotational circumferential trajectory of the measuring optical disc 602. The photoexcitation component 701 is used to emit excitation light to the mixed reagent after incubation to excite the mixed reagent to generate a light emission signal, and the detection component 702 is used to detect the light emission signal. The incubation mechanism 6 also includes an incubation shell 818603 and an incubation shell cover 604 fixedly disposed. The incubation shell cover 604 is mounted on the incubation shell 818603 and forms an incubation chamber 605 with the incubation shell 818603 to accommodate all the turntables 600. The photoexcitation component 701 and the detection component 702 are disposed on the incubation shell cover 604 and are located on the rotational circumferential trajectory of the measuring optical disc 602. The photoexcitation component 701 can emit a 680nm laser to excite the mixed reagents in the reaction cup 100, and the detection component 702 can be a PMT (photomultiplier tube) to collect photon values ​​to realize the detection and analysis of the emission signal.

[0071] In one embodiment, as shown in FIG9, the incubation mechanism 6 further includes a second positioning component 606, which is used to fix the reaction cup 100 on the measuring disc 602 facing the photoexcitation component 701 or the detection component 702. The second positioning component 606 is disposed inside the incubation shell cover 604 and located in the incubation chamber 605. The second positioning component 606 can keep the reaction cup 100 on the measuring disc 602 in the excitation position 703 and / or the detection position 704, so that the excitation light stably irradiates the mixed solution in the reaction cup 100, or the fluorescence in the reaction cup 100 can be stably collected, thereby ensuring the accuracy of the detection results.

[0072] As shown in Figure 9, the second positioning component 606 includes a fixed block 607 and a movable block 608. The fixed block 607 is fixedly connected to the incubation shell cover 604, and the movable block 608 is movably connected to the incubation shell cover 604 to move closer to or away from the fixed block 607, so that the movable block 608 can clamp the reaction cup 100 after approaching the fixed block 607. Specifically, when the test disc 602 is detected to be not rotating, the movable block 608 on the inner side of the incubation shell cover 604 clamps the reaction cup 100 after approaching the fixed block 607, so that the reaction cup 100 is held in the excitation position 703 and / or the detection position 704. When the test disc 602 is detected to be rotating, the reaction cup 100 can also continue to rotate with the test disc 602. In other variations, the movable block 608 can also be an elastic block, such as one made of silicone or rubber. When the reaction cup 100 enters between the fixed block 607 and the movable block 608, the movable block 608 is deformed by the pressure of the reaction cup 100. The movable block 608 exerts a reaction force on the reaction cup 100, so that the reaction cup 100 is stably positioned between the fixed block 607 and the movable block 608.

[0073] To improve the stability of the movable block 608 during movement, the second positioning assembly may further include a limiting block 615, which is connected to the incubation shell cover 604. Furthermore, the movable block 608 and the limiting block 615 are connected by an elastic element 616, which effectively holds the reaction cup 100 between the movable block 608 and the fixed block 607. The elastic element 616 is a spring.

[0074] As shown in Figure 10, the incubation mechanism 6 further includes an incubation reset detection unit 609 for detecting whether the incubation disk 601 is at the zero position; or, the incubation mechanism 6 further includes an incubation step counting unit 610 for detecting the rotation angle of the incubation disk 601; or, the incubation mechanism 6 further includes a photometric reset detection unit 611 for detecting whether the photometric optical disc 602 is at the zero position; or, the incubation mechanism 6 further includes a photometric step counting unit 612 for detecting the rotation angle of the photometric optical disc 602. The incubation disk 601 and the photometric optical disc 602 return to the zero position every 360° rotation. The incubation reset detection unit 609, the incubation step counting unit 610, the photometric reset detection unit 611, and the photometric step counting unit 612 can be photoelectric sensors, and their installation positions can be determined according to actual conditions.

[0075] As shown in Figure 9, a socket 613 is provided on the turntable 600. The socket 613 extends outward along the axial direction of the turntable 600. The clearance between the reaction cup 100 and the hole in the socket 613 is less than a set value, which is determined according to the wall thickness of the reaction cup 100. The socket 613 can be formed in multiple rings on the turntable 600, for example, two rings on the incubation tray 601. The extension formed by the outward extension of the socket 613 can be supported on the surface of the turntable 600, and the clearance between the reaction cup 100 and the hole in the socket 613 is less than 1 / 3 of the wall thickness of the reaction cup 100. This design allows for rapid and uniform heating of the mixed solution in the reaction cup 100, while also facilitating the handling of the reaction cup 100.

[0076] As shown in Figure 4, the incubation mechanism 6 can incubate the mixed reagents in the reaction vessel 100 once or multiple times. The detection device may also include a heating assembly 614, which is mounted on the incubation shell and used to heat the environment around the turntable 600. The heating assembly 614 includes a temperature sensor, a heating element, and a temperature control switch. The temperature sensor is used to collect the current ambient temperature to control the heating element to provide the corresponding heat source temperature, and the temperature control switch is used to provide temperature overload protection.

[0077] In one embodiment, as shown in Figures 11 to 18, the cup-gripping assembly 500 includes a gripper 504, a rotating shaft 503, a fixing plate 501, a gripper clamping spring 509, and a cam 510. The gripper 504 is used to grip the reaction cup 100. The gripper 504 is divided into a left gripper 505 and a right gripper 506, which are mounted opposite to each other on the rotating shaft 503 and rotate around the rotating shaft 503. The rotating shaft 503 is longitudinally mounted on the bottom of the fixing plate 501. One end of the gripper clamping spring 509 is connected to the left gripper 505, and the other end is connected to the right gripper 506, for clamping the left gripper 505 and the right gripper 506 together. The cam 510 is disposed between the left gripper 505 and the right gripper 506, for pushing the left gripper 505 and the right gripper 506 to the sides respectively. When gripping the reaction cup 100, the cam 510 rotates, pushing the left gripper 505 and the right gripper 506 to the left and right sides respectively, and the gripper clamping spring 509 is stretched; after the gripper 504 is moved to the gripping position of the reaction cup 100, the cam 510 rotates in the opposite direction, and the gripper 504 clamps the reaction cup 100 inward under the clamping force provided by the gripper clamping spring 509.

[0078] In one embodiment, the gripper 504 further includes a gripper 504 center positioning mechanism for keeping the right gripper 506 in constant contact with the cam 510. The gripper 504 center positioning mechanism is a gripper center positioning spring 512. One end of the gripper center positioning spring 512 is connected to the spring fixing rod 502 of the fixed plate 501, and the other end is connected to the right gripper 506. Under the pulling force provided by the gripper center positioning spring 512, the right gripper 506 keeps in constant contact with the cam 510, ensuring that the center position of the reaction cup 100 grasped by the gripper 504 is fixed. The spring fixing rod 502 is longitudinally arranged at the bottom of the fixed plate 501 near the left gripper 505. When gripping the reaction cup 100, the cam 510 rotates, pushing the left gripper 505 and the right gripper 506 to the left and right respectively, and the gripper center positioning spring 512 is stretched. After the gripper 504 is moved to the gripping position of the reaction cup 100, the cam 510 rotates in the opposite direction. Under the pulling force provided by the gripper center positioning spring 512, the right gripper 506 still maintains contact with the cam 510, thereby ensuring that the center position of the reaction cup 100 gripped by the gripper 504 is fixed.

[0079] In one embodiment, the cross-section of the cam 510 is nearly elliptical, with its diameter divided into a major axis and a minor axis. The cam 510 is an eccentric cam 510, with its shaft hole located on the major axis. The cam 510 is divided into a long end and a short end along the major axis direction via the shaft hole. When the gripper 504 opens, the long end of the cam 510 contacts the left gripper 505, and the short end of the cam 510 contacts the right gripper 506. This ensures smooth movement of the right gripper 506 when the cam 510 rotates, preventing the right gripper 506 from impacting the reaction cup 100 when gripping the cup and reducing the wobbling of the right gripper 506. In this embodiment, one end of the cam 510 along its minor axis is a plane, and the other end is a curved surface. During the clamping process of the gripper 504, the right gripper 506 remains in contact with the curved surface of the cam 510, further ensuring smooth movement of the right gripper 506.

[0080] In one embodiment, the tops of the left gripper 505 and the right gripper 506 are respectively provided with a left roller 507 and a right roller 508. The left gripper 505 and the right gripper 506 contact the cam 510 through the left roller 507 and the right roller 508 respectively, reducing the friction between the gripper 504 and the cam 510. The right roller 508 always maintains contact with the cam 510, ensuring the stability of the gripper 504, and thus ensuring that the gripper 504's cup-grabbing and cup-releasing action is smooth; when the cam 510 rotates to a certain position, the left roller 507 contacts the cam 510.

[0081] In this embodiment, the fixing plate 501 is provided with a through hole, which is circular and located at the center of the fixing plate 501. The gripper 504 also includes a motor 511, which is mounted on the top of the fixing plate 501. The rotating shaft 503 of the motor 511 passes through the through hole and is connected to the shaft hole of the cam 510 to drive the cam 510 to rotate.

[0082] The gripper 504 also includes a gripper opening sensor 513, which is mounted below the fixing plate 501 and is used to detect the degree of opening of the gripper 504. The gripper opening sensor 513 is a slot-shaped photoelectric sensor, with the slot arranged horizontally and the opening 321 facing the cam 510. The gripper 504 also includes a gripper opening sensor baffle 516, which is arranged laterally on the cam 510 and is used to block the light source in the slot of the gripper opening sensor 513 when the gripper 504 is opened to its maximum extent.

[0083] When gripping the reaction cup 100, the motor 511 is first controlled to drive the cam 510 to rotate from left to right. When the gripper opening sensor 513 detects that the gripper 504 has opened to its maximum extent, the motor 511 is controlled to stop rotating, keeping the gripper 504 open to its maximum extent. The gripper 504 is then moved directly above the reaction cup 100, and the left gripper 505 and right gripper 506 are suspended on the left and right sides of the reaction cup 100. The motor 511 is then controlled to drive the cam 510 to rotate from right to left, and the left gripper 505 and right gripper 506... Under the clamping force provided by the gripper clamping spring 509, the reaction cup 100 is clamped inward. At the same time, the right roller 508 at the top of the right gripper 506 remains in contact with the cam 510 under the pulling force provided by the gripper center positioning spring 512, ensuring that the center position of the reaction cup 100 grasped by the gripper 504 is fixed. The gripper 504 is moved to the designated position, and the control motor 511 drives the cam 510 to push the left gripper 505 and the right gripper 506 to the left and right respectively, so as to put down the reaction cup 100.

[0084] In one embodiment, as shown in Figures 17 and 18, the gripper 504 further includes a cupless sensor 514, which is mounted on the right gripper 506 and used to detect whether the gripper 504 is holding the reaction cup 100. The cupless sensor 514 is a slot-shaped photoelectric sensor. The gripper 504 also includes a cupless sensor baffle 515 mounted laterally on the left gripper 505, which can block the light source in the groove of the cupless sensor 514. In the height direction, the height of the cupless sensor baffle 515 is the same as the height of the groove of the cupless sensor 514. Therefore, the cupless sensor baffle 515 can enter the groove of the cupless sensor 514 horizontally, blocking the light source in the groove of the cupless sensor 514 and hindering the transmission of light signals in the groove of the cupless sensor 514, thereby detecting whether the gripper 504 has grasped the reaction cup 100. The cupless sensor baffle 515 is used to cooperate with the cupless sensor 514 to detect whether the gripper 504 is holding the reaction cup 100. If gripper 504 holds reaction cup 100, and left gripper 505 and right gripper 506 are clamped together, the cupless sensor baffle 515 will not enter the groove of cupless sensor 514 and will not block the light source in the groove of cupless sensor 514. If gripper 504 does not hold reaction cup 100, and left gripper 505 and right gripper 506 are clamped together, the cupless sensor baffle 515 will enter the groove of cupless sensor 514, blocking the light source in the groove of cupless sensor 514 and hindering the transmission of light signal in the groove of cupless sensor 514. This will cause cupless sensor 514 to detect that gripper 504 has not held reaction cup 100 or that reaction cup 100 has fallen from gripper 504 during movement.

[0085] As is well known, the pipette 400 and the gripper cup assembly 500, as actuating components, cannot move in space. Therefore, the pipette 400 is equipped with a first drive assembly 401 and the gripper cup assembly 500 is equipped with a second drive assembly 517 in order to achieve the purpose of moving in space.

[0086] In one embodiment, considering that the cup-gripping assembly 500 moves in a two-dimensional plane, and in order to place the reaction cup 100 on the turntable 600, naturally one direction is vertical and the other is horizontal. Therefore, as shown in FIG3, the second driving assembly 517 includes a vertical driving member 519 and a fixedly disposed horizontal driving member 518. The vertical driving member 519 is assembled on the horizontal driving member 518 and is controlled by the horizontal driving member 518 to move in the horizontal direction. The cup-gripping assembly 500 is assembled on the vertical driving member 519 and is controlled by the vertical driving member 519 to move in the vertical direction.

[0087] As shown in Figure 3, taking the vertical drive component 519 as an example, the vertical drive component 519 includes a drive motor 520, a first pulley, a second pulley 521, and a belt 522. The belt is fixedly connected to the cup-gripping assembly 500 and is also meshed with the first pulley and the second pulley 521. The first pulley and the second pulley 521 rotate synchronously under the control of the drive motor 520 to drive the belt 522 to move, and thus drive the cup-gripping assembly 500 to move in the vertical direction. Furthermore, the vertical drive component 519 includes a fixedly installed vertical slide rail 523. This fixed installation refers to the fixed part of the vertical drive component 519; for example, the belt 522 connected to the cup-gripping assembly 500 is considered a movable part. The cup-gripping assembly 500 slides on the vertical slide rail 523. In this embodiment, the cup-gripping assembly 500 can be supported by the auxiliary vertical slide rail 523 to move along the moving direction of the belt 522. Considering that the form of the driving component is known to those skilled in the art, and that the movement in three-dimensional space is simply the same or similar to the movement in two-dimensional plane by adding a new driving component, other variations and embodiments of the first driving component 401 will not be described in detail.

[0088] As shown in Figure 3, the cup-grabbing mechanism 5 also includes a horizontal step-loss detection component 524. The horizontal step-loss detection component 524 includes a first code disk 525 and a first optical coupler 526. The movement of the first code disk 525 is synchronized with the horizontal movement of the cup-grabbing component 500. The first optical coupler 526 remains stationary when the first code disk 525 moves, and detects whether the horizontal movement of the cup-grabbing component 500 has lost steps based on the evenly distributed code teeth on the first code disk 525.

[0089] In one embodiment, the cup-grabbing mechanism 5 further includes a vertical step-loss detection component 527, which includes a second code disk 528 and a second optical coupler 529. The movement of the second code disk 528 is synchronized with the vertical movement of the cup-grabbing component 500. The second optical coupler 529 remains stationary when the second code disk 528 moves, and detects whether the vertical movement of the cup-grabbing component 500 has lost a step based on the evenly distributed code teeth on the second code disk 528.

[0090] The first code disk 525 and the second code disk 528 are gear-shaped or comb-shaped. As shown in Figure 3, in this embodiment, the first code disk 525 is gear-shaped, and the second code disk 528 is comb-shaped. The first code disk 525 is mounted on the second pulley 521 and rotates synchronously with the second pulley 521. The first optocoupler 526 is mounted on the frame 1 and determines whether a step is missed based on the rotation information of the code teeth on the first code disk 525. The second code disk 528 is fixedly connected to the cup gripping assembly 500 and moves up and down together with the cup gripping assembly 500. The second optocoupler 529 is provided on the fixed part of the vertical drive member 519 and determines whether a step is missed based on the movement information of the code teeth on the second code disk 528. In one embodiment, a zero-position optocoupler 530 is also provided on the fixed part of the vertical drive member 519, which determines the initial height of the cup gripping assembly 500.

[0091] Similarly, for the step loss detection component in the pipetting mechanism 4, a step loss detection component in the corresponding direction can be set according to different movement directions. Its working structure and principle are similar to the horizontal step loss detection component 524 / vertical step loss detection component 527 in the cup gripping mechanism 5, so it will not be described in detail again.

[0092] In one embodiment, as shown in FIG1, the detection device further includes a cup feeding mechanism 101 and a cup pushing mechanism 104. The cup feeding mechanism 101 is used to store the reaction cup 100 and transfer it to the cup pushing mechanism 104. The cup pushing mechanism 104 is used to push the reaction cup 100 onto the turntable 600.

[0093] In this embodiment, the reaction cup 100 first reaches the turntable 600 under the combined action of the cup feeding mechanism 101 and the cup pushing mechanism 104. In addition to the cup gripping mechanism 5, the cup feeding mechanism 101 and the cup pushing mechanism 104 are set separately. The reaction cup 100 can be periodically supplied to the turntable 600 through the control of the cup pushing mechanism 104, thereby realizing orderly and continuous photo-induced chemiluminescence immunoassay detection. The cup feeding mechanism 101 may include a cup feeding chamber 102 and a cup feeding channel 103. The cup feeding chamber 102 may be located at the top of the frame 1. The cup feeding chamber 102 stores multiple randomly arranged reaction cups 100 poured in by the operator. The cup feeding channel 103 receives the reaction cups 100 that fall from the cup feeding chamber 102 under the action of gravity and sorts the disordered reaction cups 100 on the cup feeding channel 103. The reaction cups are then conveyed to the cup pushing mechanism 104 through the cup feeding channel 103. The cup pushing mechanism 104 can push the reaction cups 100 to the turntable 600 in the incubation mechanism 6 at a set frequency. It should be noted that the cup feeding chamber 102, the cup feeding channel 103, and the cup pushing mechanism 104 can all be implemented using any existing technology, and therefore will not be described in detail.

[0094] In one embodiment, as shown in Figures 19 to 23, the reagent module 3 is provided with a first reagent compartment 301 for storing a first reagent. The top of the first reagent compartment 301 is provided with a liquid dispensing port 302. The detection device also includes a sealing mechanism 303. The sealing mechanism 303 includes a cover plate 304 with a first through hole 305 and a third driving component 306 connected to the cover plate 304. The cover plate 304 is slidably disposed on the top of the first reagent compartment 301 and moves back and forth on the first reagent compartment 301 under the drive of the third driving component 306, so that the first through hole 305 and the liquid dispensing port 302 are connected to the inside and outside of the first reagent compartment 301 or are staggered and separated from the inside and outside of the first reagent compartment 301.

[0095] The cover plate 304 is provided with a first through hole 305, and the cover plate 304 is slidably connected to the first reagent chamber 301; the third driving component 306 is used to drive the cover plate 304 to slide back and forth between a first position and a second position relative to the first reagent chamber 301; in Figure 21, the cover plate 304 is located in the first position. When the cover plate 304 is located in the first position, the first through hole 305 is offset from the liquid outlet 302 so that the cover plate 304 can close the liquid outlet 302; in Figure 22, the cover plate 304 is located in the second position. When the cover plate 304 is located in the second position, the first through hole 305 is arranged opposite to the liquid outlet 302. In this embodiment, when the first reagent is not needed, the third driving component 306 positions the cover plate 304 in a first position, with the first through hole 305 on the cover plate 304 offset from the liquid dispensing port 302, so that the cover plate 304 can close the liquid dispensing port 302, ensuring the airtightness of the first reagent chamber 301. When the first reagent is being drawn, the third driving component 306 positions the cover plate 304 in a second position, with the first through hole 305 opposite to the liquid dispensing port 302, so that the tip can be inserted from the outside into the reagent bottle inside the liquid dispensing port 302. Therefore, in this embodiment, the liquid dispensing port 302 can be opened only when liquid is being drawn, and kept closed when liquid is not needed, ensuring the refrigeration effect of the first reagent chamber 301 on the first reagent and avoiding the situation where the refrigeration temperature is not up to standard. In this embodiment, the cover plate 304 can be slidably connected to the first reagent chamber 301 via a guide rail 314, which is located between the cover plate 304 and the first reagent chamber 301.

[0096] In one embodiment, the first reagent compartment 301 has multiple liquid dispensing ports 302, and the cover plate 304 is adapted to have multiple first through holes 305, wherein each liquid dispensing port 302 corresponds to one first through hole 305. A reagent bottle is provided below each liquid dispensing port 302 in the first reagent compartment 301. When the cover plate 304 is in the second position, the bottle mouth, liquid dispensing port 302 and first through hole 305 are arranged in concentric circles. The tip can pass through the first through hole 305, the liquid dispensing port 302 and the bottle mouth of the reagent bottle in sequence to enter the reagent bottle to draw liquid.

[0097] As shown in Figures 19 to 22, the sealing mechanism 303 also includes a position sensor 307 for detecting the relative positions of the first through-hole 305 and the liquid inlet 302. The position sensor 307 is triggered when the third drive assembly 306 drives the cover plate 304 to the second position. In one embodiment, the position sensor 307 may be a photoelectric sensor, disposed on the first reagent chamber 301. When the cover plate 304 moves, triggering the position sensor 307 indicates that the cover plate 304 is in the second position, and the third drive assembly 306 stops driving the cover plate 304 to continue moving, thus keeping the cover plate 304 in the second position.

[0098] In one embodiment, as shown in FIG23, the sealing mechanism 303 further includes a heating plate 308 attached to the cover plate 304, and the heating plate 308 has a second through hole 309 corresponding to the first through hole 305. To solve the problem of condensation contamination and reagent dilution at the top of the first reagent chamber 301, the sealing mechanism 303 also includes a heating plate 308 on the cover plate 304. The heating plate 308 can heat the cover plate 304, making its temperature close to the external ambient temperature of the first reagent chamber 301. This prevents condensation from forming on the cover plate 304 due to the cold air outside, and also prevents condensation from falling into the reagent bottle when the cover plate 304 is in the second position, thus preventing contamination and reagent dilution. The cover plate 304 has multiple first through holes 305, the heating plate 308 has a sheet-like structure, and the heating plate 308 has multiple second through holes 309, which are arranged one-to-one with the multiple first through holes 305 for the liquid collection needle to pass through. The heating plate 308 is a resistance heating element.

[0099] In one embodiment, the sealing mechanism 303 further includes a heat insulation plate 310 attached to the cover plate 304, and the heat insulation plate 310 has a third through hole 311 corresponding to the first through hole 305. In this embodiment, the heat insulation plate 310 can prevent the heat generated by the heating plate 308 from being lost.

[0100] In one embodiment, the sealing mechanism 303 further includes a heating plate 308 and a heat-insulating plate 310 attached to a cover plate 304. The heating plate 308 is located between the cover plate 304 and the heat-insulating plate 310, and the heating plate 308 and the heat-insulating plate 310 are sequentially provided with second through holes 309 and third through holes 311 corresponding to the first through hole 305. The heat-insulating plate 310 is used to cover the heating plate 308 on the cover plate 304 to prevent the heat generated by the heating plate 308 from being lost. Additionally, a chamber for installing the heating plate 308 can be provided at the upper end of the cover plate 304. The heating plate 308 can be fixed to the cover plate 304 with bolts, and the heat-insulating plate 310 covers the heating plate 308. The heat-insulating plate 310 is provided with multiple third through holes 311, which correspond one-to-one with multiple second through holes 309, for the passage of a liquid-taking needle.

[0101] As shown in Figure 23, the sealing mechanism 303 also includes a temperature sensor 312 and an over-temperature protector 313 located on the outside of the cover plate 304, and both the temperature sensor 312 and the over-temperature protector 313 are located away from the third drive assembly 306. The sealing mechanism 303 also includes an over-temperature protector 313 located on the cover plate 304, which is located to one side of the temperature sensor 312. When the temperature sensor 312 is damaged, the heating plate 308 will continue to heat. When the temperature reaches the over-temperature protection set temperature, the power supply to the heating plate 308 will be disconnected to stop heating, thus providing protection.

[0102] In one embodiment, as shown in Figures 24 to 27, the reagent module 3 further includes a reagent compartment 300 for storing reagents. The detection device also includes a heat dissipation mechanism 314 located outside the reagent compartment 300. The heat dissipation mechanism 314 is used to regulate the temperature of the reagents stored in the reagent compartment 300. The heat dissipation mechanism 314 can be located outside the reagent compartment 300 to regulate the temperature of the stored reagents without affecting the retrieval and placement of the stored reagents. The reagent compartment 300 can store either a first reagent or a second reagent.

[0103] As shown in Figures 25 and 27, the heat dissipation mechanism 314 includes a semiconductor cooling unit 315, a heat dissipation housing 316, an air inlet component, and an air outlet component. The heat dissipation housing 316 has a receiving cavity 319 and a heat dissipation duct 320. The air inlet component and the air outlet component are located at both ends of the heat dissipation duct 320 to form a duct along a predetermined airflow direction. The receiving cavity 319 houses the semiconductor cooling unit 315, and a portion of the receiving cavity 319 is located within the heat dissipation duct 320. The hot end of the semiconductor cooling unit 315 is close to the heat dissipation duct 320, and the cold end is away from the heat dissipation duct 320. The cold end of the semiconductor cooling unit 315 is in close contact with the reagent compartment 300 through a corresponding opening 321 on the heat dissipation housing 316. In this embodiment, the receiving cavity 319 in the heat dissipation housing 316 is at least partially located within the heat dissipation duct 320. The receiving cavity 319 may be located between the air inlet 317 and the air outlet 318, or it may be located upstream of the air inlet 317 or downstream of the air outlet 318. This application does not limit the position of the receiving cavity 319. The air inlet component and the air outlet component may be a fan to keep the airflow direction of the heat dissipation duct 320 uniform. However, the space of the receiving cavity 319 and the space of the heat dissipation duct 320 overlap at least partially, so that the airflow in the heat dissipation duct 320 can pass through the semiconductor cooling unit 315 in the receiving cavity 319 to dissipate heat from the semiconductor cooling unit 315.

[0104] In the heat dissipation mechanism 314, the semiconductor cooling unit 315 performs cooling, and the airflow in the heat dissipation duct 320 dissipates heat from the semiconductor cooling unit 315. During use, the cold end of the semiconductor cooling unit 315 is brought close to the reagent chamber 300 to cool the reagent chamber 300. Since the hot end of the semiconductor cooling unit 315 generates heat during the cooling process, the airflow dissipates heat from the hot end of the semiconductor cooling unit 315.

[0105] As shown in Figures 25 and 26, the semiconductor cooling unit 315 includes a semiconductor cooling chip 322 and a heat dissipation fin assembly 323. The cold end of the semiconductor cooling chip 322 is away from the heat dissipation duct 320, while the hot end is close to the heat dissipation duct 320. The hot end is also connected to the heat dissipation fin assembly 323. The cold end of the semiconductor cooling chip 322 is connected to the reagent chamber 300 through an opening 321 on the heat dissipation housing 316 to cool the reagent chamber 300. The heat dissipation fins are placed at the hot end of the semiconductor cooling chip 322 to transfer the heat generated at the hot end. The heat dissipation fins are placed inside the heat dissipation duct 320, and the airflow inside the heat dissipation duct 320 carries away the heat on the heat dissipation fins, thereby achieving heat dissipation at the hot end of the semiconductor cooling chip 322 and improving heat dissipation efficiency.

[0106] In one embodiment, the heat dissipation fins in the heat dissipation fin assembly 323 are arranged at intervals along the airflow direction within the heat dissipation duct 320. As shown in FIG25, the airflow direction within the heat dissipation duct 320 flows from left to right, and the heat dissipation fins are also distributed from left to right, thereby increasing the contact area between the heat dissipation fins and the airflow within the heat dissipation duct 320 and improving heat dissipation efficiency. The heat dissipation mechanism 314 includes multiple semiconductor cooling units 315, which are arranged at intervals along the airflow direction within the heat dissipation duct 320. The cold sides of the multiple semiconductor cooling units 315 are in close contact with the reagent compartment 300. By setting multiple semiconductor cooling units 315, the temperature of the stored reagents within the reagent compartment 300 can be efficiently regulated.

[0107] In one embodiment, a tip reuse module is also included, located between the injection module and the waste area, enabling the reuse of reagents during pipetting. The tip reuse module is not labeled in the accompanying drawings, but its structure is known to those skilled in the art and will not be described in detail here.

[0108] To better illustrate the technical solution of this application, the following description is based on a detection process of an embodiment.

[0109] The reaction cup 100 is conveyed from the top of the rack 1 to the incubation tray 601 by the cup feeding mechanism 101 and the cup pushing mechanism 104. The incubation tray 601 is rotated to transfer the reaction cup 100 to the sample dispensing position. After the pipette 400 is connected to the disposable pipette tip (TIP), it draws the sample from the sample tube at the sample tube carrier and injects the drawn sample into the reaction cup 100 at the sample dispensing position. The pipette 400 discards the TIP in the waste box 105. After that, the pipetting mechanism 4 connects to a new tip, and the cover plate 304 of the sealing mechanism 303 at the top of the first reagent compartment 301 moves to the second position to draw the first reagent into the reaction cup 100. The first reagent can be a single component or multiple components, which can be determined according to actual needs. If the same tip will be used again, the tip can be temporarily stored in the tip reuse module. After the sample addition process is completed, the incubation tray 601 rotates and moves the reaction cup 100 to a designated position so that the cup gripping assembly 500 can move downward to grip the reaction cup 100. The cup gripping assembly 500 grips the reaction cup 100 and transfers it to the mixing position in the incubation mechanism 6 for mixing. After mixing is completed, the cup gripping assembly 500 grips the reaction cup 100 and places it on the incubation position of the incubation tray 601 for the first incubation. After the first incubation, the cup-grabbing assembly 500 grasps the reaction cup 100 on the incubation tray 601 and places it on the corresponding injection position 812 on the measuring disc 602 to add the second reagent, and then performs the second incubation; wherein, the pipette 400 moves and extends into the drive hole 816 on the seat 814 of the injection base 803 of the injection assembly 8, driving the injection base 803 to move to the corresponding injection position 812, and the second reagent is injected into the reaction cup 100 from the injection end 806. After the injection is completed, the injection base 803 is moved back, and the second reagent can be a universal liquid. After the second incubation, the incubation disc 601 rotates, moving the reaction cup 100 to the excitation position 703 below the photoexcitation mechanism to excite the mixed reagent to generate a light emission signal. Within 200ms, the inner disc rotates to transfer the reaction cup 100 to the detection position 704 below the detection mechanism 7. The detection mechanism 7 measures the photon value of the mixed reagent and transmits the collected photon value to the terminal software for calculating the concentration of the converted sample. After the reaction cup 100 completes photometry, the photometry disc 602 rotates and transfers the reaction cup 100 to a designated position for the cup-grabbing assembly 500 to grab and throw the reaction cup 100, causing it to fall into the waste box 105.

[0110] The addition of the second reagent can also be based on the actual process and is not limited to the description above.

[0111] As shown in Figures 1 to 27, this application embodiment also provides a photo-induced chemiluminescence detection method, which is based on the above-mentioned photo-induced chemiluminescence detection device, including:

[0112] Step S101: The first drive component 401 in the pipetting mechanism 4 controls the pipette 400 to connect to the disposable pipette tip. Under the drive of the first drive component 401, the disposable pipette tip transfers the sample and the first reagent stored in the aspirated disposable pipette tip to the corresponding reaction cup 100 to obtain a mixed reagent. After the transfer, the pipette 400 discards the aspirated disposable pipette tip.

[0113] Step S102: According to the process requirements, the gripping cup component 500 in the gripping cup mechanism 5, driven by the second driving component 517, positions the gripped reaction cup 100 containing the mixed reagent, so that the incubation mechanism 6 incubates the mixed reagent in the reaction cup 100; wherein, for the secondary incubation, after the first incubation, the liquid injection component 8 can complete the injection of the second reagent into the reaction cup 100 inside the incubation mechanism 6 at a specific position for the second incubation;

[0114] Step S103: The detection unit 7 performs photoexcitation on the incubated mixed reagent and detects the chemiluminescence produced by the mixed reagent after photoexcitation;

[0115] Step S104: After detecting the mixed reagent, the cup-grabbing mechanism 5 performs a cup-throwing process on the corresponding reaction cup 100.

[0116] The photo-induced chemiluminescence detection method provided in this application embodiment uses a disposable pipette tip to transfer the sample or first reagent into the reaction cup 100. After transferring the sample or reagent, the pipette mechanism 4 controls the pipette 400 to throw the corresponding disposable pipette tip, which can eliminate the need for cleaning the conventional pipette mechanism 4 for aspirating the sample or first reagent, save analysis and detection time, shorten the detection cycle, and improve detection efficiency.

[0117] Furthermore, the position of the reaction cup 100 can be scheduled by the cup-grabbing component 500 in the cup-grabbing mechanism 5. The cup-grabbing component 500 can cooperate with the turntable 600 in the incubation mechanism 6. Compared with the fact that the scheduling of the reaction cup 100 is all realized in the incubation mechanism 6, the internal complexity of the incubation mechanism 6 is reduced, thereby improving the effective utilization rate of the incubation mechanism 6. The volume of the incubation mechanism 6 can be relatively reduced to maintain the consistency of the incubation environment inside the incubation mechanism 6, improve the incubation quality, and thus improve the quality of the detection results.

[0118] Regarding the methods in the above embodiments, the implementation methods of each step have been described in detail in the embodiments of the relevant detection equipment, and will not be elaborated further here.

[0119] The various embodiments of this application have been described above. These descriptions are exemplary and not exhaustive, nor are they limited to the disclosed embodiments. Many modifications and variations will be apparent to those skilled in the art without departing from the scope and spirit of the described embodiments. The terminology used herein is chosen to best explain the principles, practical application, or improvement of the technology in the market, or to enable others skilled in the art to understand the embodiments disclosed herein.

Claims

1. A photo-induced chemiluminescence detection device, characterized in that, include: A sample introduction module, which is at least used to store a sample tube holder and a disposable pipette tip, wherein the sample tube contains a sample; The reagent module is used to store the first and second reagents; A pipetting mechanism includes a first driving component and a pipette. The pipette is mounted on the first driving component and can move in three-dimensional space under the drive of the first driving component. The pipette can be connected to a disposable pipette tip to realize the aspiration and transfer of sample and first reagent. A cup-grabbing mechanism includes a second drive component and a cup-grabbing component, wherein the cup-grabbing component is mounted on the second drive component; the cup-grabbing component can be used to grab a reaction cup and perform position scheduling on the grabbed reaction cup under the drive of the second drive component; An incubation apparatus comprising at least one turntable for loading a plurality of reaction cups along its circumference; the incubation apparatus incubating a mixture of reagents in the reaction cups; The detection mechanism is located on the incubation mechanism and is used to photoexcite the incubated mixed reagent and detect the chemiluminescence produced by the mixed reagent after photoexcitation. And a liquid injection assembly, which can complete the injection of a second reagent into the reaction cup inside the incubation mechanism at a specific location.

2. The photo-induced chemiluminescence detection device according to claim 1, characterized in that, The liquid injection assembly includes a liquid storage section, a liquid injector, a liquid injection base, an inlet pipe, and an outlet pipe; The liquid storage section is located in the reagent module and is used to store at least the second reagent; The liquid inlet pipe connects the inlet of the injector to the liquid storage section, and the liquid outlet pipe connects to the outlet of the injector and is partially coiled inside the injection base.

3. The photo-induced chemiluminescence detection device according to claim 2, characterized in that, The injection base is movably mounted on the incubation mechanism and is detachably connected to the pipette. When the injection base is connected to the pipette, the pipette, driven by the first driving component, moves the injection end of the outlet pipeline to the corresponding injection position so that the second reagent can be injected into the corresponding reaction cup in the incubation mechanism through the injection device.

4. The photo-induced chemiluminescence detection device according to claim 2, characterized in that, The injection assembly further includes a first switching component, which includes two first stationary ends and one first moving end. The first moving end is connected to the injection device, and the two first stationary ends are respectively connected to the inlet pipe and the outlet pipe.

5. The photo-induced chemiluminescence detection device according to claim 3 or 4, characterized in that, The liquid storage unit includes N+1 containers, and the liquid injection assembly further includes N second switching components, where N is a positive integer. The second switching component includes two second stationary ends and one second moving end. N second switching components are connected in series. The remaining N+1 second stationary ends are each connected to one of the containers. The remaining second moving end is connected to the injector through the liquid inlet pipe.

6. The photo-induced chemiluminescence detection device according to claim 5, characterized in that, The injection assembly also includes a waste discharge position. When the injection end is in the waste discharge position, the injection assembly is used to discharge the cleaning reagent from the container through the outlet pipe; or... When the injection end is in the injection position, the injection assembly is used to inject a second reagent from the substrate container into the reaction cup.

7. The photo-induced chemiluminescence detection device according to claim 2, characterized in that, The injection base includes: A base body, which is slidably mounted on the top of the incubation mechanism, and the base body is provided with a connecting part adapted to the pipette, the connecting part having a drive hole; The liquid injection assembly also includes an injection position and a waste discharge position. The top of the pipette is inserted into the drive hole under the drive of the first drive assembly, so that the first drive assembly drives the base to reciprocate between the injection position and the waste discharge position.

8. The photo-induced chemiluminescence detection device according to claim 2, characterized in that, The liquid injection assembly also includes a temperature control assembly disposed on the liquid injection base; The temperature control assembly includes a housing and a heating element disposed in the housing. The housing is disposed on one side of the liquid injection base, and the inner side of the housing is provided with a pipeline channel for accommodating the liquid outlet pipeline. The liquid injection end of the liquid outlet pipeline extends from the pipeline channel to the outside of the housing.

9. The photo-induced chemiluminescence detection device as described in claim 1, characterized in that, The number of turntables is M, where M is a positive integer greater than 1. If M = 2, then the two turntables are an incubation disc and a measuring disc. The incubation disc and the measuring disc are coaxially nested and each rotates independently around its own axis.

10. The photo-induced chemiluminescence detection device as described in claim 9, characterized in that, The detection mechanism includes a photoexcitation component and a detection component disposed on the incubation mechanism. The photoexcitation component and the detection component are located on the same rotating circumferential trajectory of the measuring optical disc. The photoexcitation component is used to emit excitation light to the incubated mixed reagent to excite the mixed reagent to generate a light emission signal, and the detection component is used to detect the light emission signal.

11. The photo-induced chemiluminescence detection device as described in claim 10, characterized in that, The incubation mechanism further includes a second positioning component for fixing the reaction cup on the measuring optical disc toward the photoexcitation component or the detection component.

12. The photo-induced chemiluminescence detection device as described in claim 1, characterized in that, The cup-gripping assembly includes a gripper, a rotating shaft, a fixing plate, a gripper clamping spring, and a cam; The gripper is used to grasp the reaction cup; The gripper is divided into a left gripper and a right gripper, which are mounted opposite each other on the rotating shaft and rotate around the rotating shaft; The rotating shaft is longitudinally mounted on the bottom of the fixed plate; One end of the gripper clamping spring is connected to the left gripper, and the other end is connected to the right gripper, for use in clamping the left gripper and the right gripper together; The cam is positioned between the left gripper and the right gripper, and is used to push the left gripper and the right gripper open to both sides respectively.

13. The photo-induced chemiluminescence detection device as described in claim 12, characterized in that, The cup-gripping assembly also includes a gripper center positioning component for keeping the right gripper in constant contact with the cam.

14. The photo-induced chemiluminescence detection device according to claim 1, characterized in that, The reagent module is provided with a first reagent compartment for storing the first reagent, and the top of the first reagent compartment is provided with a liquid dispensing port. The detection device also includes a sealing mechanism. The sealing mechanism includes a cover plate with a first through hole and a third driving component connected to the cover plate. The cover plate is slidably mounted on the top of the first reagent chamber and moves back and forth on the first reagent chamber under the drive of the third driving component, so that the first through hole communicates with the liquid dispensing port to the inside and outside of the first reagent chamber or is staggered and separated from the inside and outside of the first reagent chamber.

15. The photo-induced chemiluminescence detection device according to claim 1, characterized in that, The reagent module is also provided with a reagent compartment for storing reagents, and the detection device also includes a heat dissipation mechanism located outside the reagent compartment, which is used to regulate the temperature of the reagents stored in the reagent compartment.

16. The photo-induced chemiluminescence detection device according to claim 1, characterized in that, Also includes: The Tip reuse module, located between the injection module and the waste area, enables the reuse of reagents during pipetting.

17. A photo-induced chemiluminescence detection method, based on the photo-induced chemiluminescence detection device according to any one of claims 1 to 16, characterized in that, include: The first drive component in the pipetting mechanism controls the pipette to connect to the disposable pipette tip. The disposable pipette tip is driven by the first drive component to transfer and aspirate the sample and the first reagent into the corresponding reaction cup to obtain a mixed reagent. The pipette discards the aspirated disposable pipette tip after transfer. According to the process requirements, the cup-grabbing component in the cup-grabbing mechanism, driven by the second drive component, positions the grasped reaction cup containing the mixed reagent, so that the incubation mechanism incubates the mixed reagent in the reaction cup; wherein, for secondary incubation, after the first incubation, the liquid injection component can complete the injection of the second reagent into the reaction cup inside the incubation mechanism at a specific position for the second incubation. The testing agency performs photoexcitation on the incubated mixed reagent and detects the chemiluminescence produced by the mixed reagent after photoexcitation; The cup-grabbing mechanism performs a cup-throwing process on the corresponding reaction cups after detecting the mixed reagents.

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