Plasma preparation device

Abstract

A plasma preparation device (100). The plasma preparation device (100) comprises: a first assembly (20), one end of the first assembly (20) being provided with a first rotary cap (21); a second assembly (30), comprising a first portion (31) and a second portion (33), the first portion (31) being rotatably sleeved on the first assembly (20), the second portion (33) being in communication with the first portion (31), an adjustment portion (311) being provided on a side wall of the first portion (31), and a first opening (26) being provided on a side wall of the first assembly (20); an outer tube (40), which covers the second assembly (30) in a liquid-tight manner; and a sealing member (50), which is provided below the second assembly (30) in a liquid-tight manner.

Description

Plasma preparation device

[0001] Related applications

[0002] The present application claims priority to Chinese Patent Application No. 202421282983.8, filed on June 5, 2024, entitled "A Platelet-Rich Plasma Preparation Device", and Chinese Patent Application No. 202411232864.6, filed on September 3, 2024, entitled "A Leukocyte-Depleted PRP Preparation Device and Method", the contents of which are hereby incorporated by reference in their entirety. TECHNICAL FIELD

[0003] The present application relates to the technical field of medical devices, in particular to a platelet-rich plasma preparation device. BACKGROUND

[0004] After centrifugation, blood will be stratified, the upper layer is a platelet-poor plasma (PPP) layer, the middle layer is a platelet-rich plasma (PRP) layer containing a large number of platelets and a small amount of white blood cells, and the lower layer is a red blood cell layer containing a large number of red blood cells and part of white blood cells. Platelet-rich plasma (PRP) is a product obtained by centrifuging whole blood from the human body, which has a high concentration of platelet components and is widely used in bone injury, sports injury, wound healing, tissue regeneration and other departments. According to the different concentrations of white blood cells contained in the prepared PRP, PRP can be divided into leukocyte-poor PRP (P-PRP) and leukocyte-rich PRP (L-PRP). Some studies have shown that the high concentration of white blood cells in L-PRP can cause an inflammatory response, which offsets the beneficial effects of growth factors in PRP. The use of P-PRP can reduce the concentration of inflammatory factors and reduce the inflammatory response, thereby accelerating tissue regeneration. SUMMARY

[0005] According to various embodiments of the present application, a plasma preparation device is provided.

[0006] In one aspect of the present application, a plasma preparation device is provided. The plasma preparation device comprises: a first assembly provided with a first rotary cap at one end, a second assembly, a first part and a second part, the first part being rotatably sleeved on the first assembly, and the second part being communicated with the first part, wherein a regulating part is arranged on the side wall of the first part, a first opening is arranged on the side wall of the first assembly, and the first rotary cap can drive the first assembly to rotate synchronously to adjust the position between the first opening and the regulating part; an outer tube liquid-tightly covers the second assembly; a sealing member is liquid-tightly arranged below the second assembly, and the sealing member is at least partially movable up and down along the axial direction of the plasma preparation device.

[0007] The plasma preparation device provided by the application can adjust the amount of PPP flowing out of the second component through the first component controlling the switch of the adjusting part, so as to adjust the remaining amount of PPP in the second component, and further adjust the total amount of platelet-rich plasma and platelet-poor plasma, and further adjust the concentration multiple of platelet-rich plasma. The plasma preparation device provided by the application can realize the adjustable concentration multiple of platelet-rich plasma, so that platelet-rich plasma with different concentration multiples can be prepared according to different requirements. At the same time, the plasma preparation device provided by the application has a compact structure, and occupies a small space during use. In addition, by arranging the outer tube, the part of PPP to be separated can be accommodated, so that the PPP can be recovered.

[0008] In some embodiments, the adjusting part is at least one second opening, and the at least one second opening is arranged along the circumferential direction of the first part.

[0009] In some embodiments, the number of second openings is a plurality, and the plurality of second openings are arranged in a stepped manner on the peripheral wall of the first component; and the first opening is arranged along the height direction of the plasma preparation device.

[0010] In some embodiments, the number of second openings is one, the shape of the second opening is triangular, and the first opening is arranged along the height direction of the plasma preparation device.

[0011] In some embodiments, the adjusting part is a second opening extending along the peripheral wall of the first component, and the first opening and the second opening intersect, and when the first rotating cover rotates relative to the second component, the intersection between the first opening and the second opening moves up and down relative to the height direction of the plasma preparation device.

[0012] In this way, the intersection between the first opening and the second opening moves up and down relative to the height direction of the plasma preparation device, and then part of the PPP is separated, so that the occurrence of the situation affecting the concentration multiple of the prepared red blood cell-poor PRP is affected.

[0013] In some embodiments, the plasma preparation device further comprises an extraction component which is detachably connected to the first rotating cover and is used for extracting liquid in the second component.

[0014] In this way, the PPP can be extracted from the second component by the extraction component, and the red blood cell-poor PRP with the target concentration multiple can be prepared.

[0015] In some embodiments, the first opening is a straight groove extending straightly on the peripheral wall of the first rotating cover, and the second opening is a spiral groove extending curvedly on the peripheral wall of the first part.

[0016] In this way, the angle of rotating the first screw cap relative to the second assembly is linearly related to the distance of moving up and down at the intersection position, so as to accurately separate the part of PPP and reduce errors.

[0017] In some embodiments, the central angle of the spiral groove is less than 180°.

[0018] In this way, the first opening and the second opening can be non-intersected, so as to prevent blood leakage when the red blood cell-poor PRP preparation device is placed in a centrifuge for extraction.

[0019] In some embodiments, the second assembly further comprises a throat portion, which is in communication between the first portion and the second portion, and the cross-sectional area of the throat portion is smaller than the cross-sectional area of the first portion and the cross-sectional area of the second portion, respectively.

[0020] In this way, compared with the scheme without the throat portion, the scheme with the throat portion, because the cross-sectional area of the throat portion is smaller than the cross-sectional area of the inner cavity, reduces the mixing of the liquids contained in the inner cavity due to vibration when the PRP layer and the PPP layer are extracted by means of the extraction assembly, thereby affecting the quality of the obtained PRP.

[0021] In some embodiments, the outer tube is detachably connected to the first screw cap, and one end of the outer tube is sealingly connected to the first screw cap.

[0022] In some embodiments, the ratio of the sum of the volume of the cavity surrounded by the first assembly and the first portion to the volume of the second portion is 1:1.2-1:1.

[0023] In some embodiments, the sealing member comprises: a sealing piece, which moves up and down along the axis of the plasma preparation device, and a plurality of annular protrusions are arranged on the outer wall of the sealing piece and abut against the inner wall of the second portion; a second screw cap, which is connected to the sealing piece; and the screw cap and the second portion are connected by threads.

[0024] The sealing member with this structure is compact in structure, small in space occupation, reliable in connection, and stable in movement.

[0025] In some embodiments, the outer tube is detachably connected to the first screw cap and the second portion, one end of the outer tube is sealingly connected to the first screw cap, and the other end of the outer tube is sealingly connected to the second portion away from the end connected to the first screw cap.

[0026] In some embodiments, the sealing member further comprises a second screw cap, which is threadedly connected to the outer tube, and the second screw cap carries the sealing member.

[0027] In this way, the position of the sealing member and the second screw cap relative to the outer tube can be adjusted by using the second screw cap, so as to adjust the liquid level in the second assembly relative to the cavity, thereby preventing the extraction assembly from being unable to completely extract the PRP layer due to insufficient volume of the liquid in the second assembly when the PRP layer and the PPP layer are extracted by using the extraction assembly, and finally causing insufficient platelet content in the prepared red blood cell-depleted PRP.

[0028] In some embodiments, the first screw cap is provided with a vent hole, and the vent hole is provided with a filter structure.

[0029] Through the vent hole, the inner cavity and the overflow part can communicate with the external environment, so as to achieve internal and external pressure balance; the filter structure can prevent bacteria from entering the inner cavity and the overflow part.

[0030] In some embodiments, the first screw cap has a flow guide pipe, and the extraction assembly is inserted into the flow guide pipe.

[0031] In some embodiments, the extraction assembly comprises a syringe and an internal channel switch, the internal channel switch is connected to the end of the syringe away from the first screw cap, and can move up and down with the syringe, the internal channel switch comprises a pressure head and an anti-fouling block, the pressure head can move up and down in the flow guide pipe, and the anti-fouling block contacts the flow guide pipe to block the port of the end of the flow guide pipe away from the first screw cap.

[0032] In this way, the flow guide pipe can prevent blood from contacting external substances under unnecessary conditions when the extraction assembly is stored, thereby preventing blood contamination.

[0033] In some embodiments, the pressure head comprises a liquid outlet filter head having a liquid outlet, a liquid inlet filter head having a liquid inlet, and a white blood cell filter structure, which is arranged between the liquid outlet filter head and the liquid inlet filter head.

[0034] In some embodiments, the first screw cap is provided with a third opening, which communicates with the inside of the first assembly, and the first screw cap is also provided with a fourth opening, which is arranged at a position between the second assembly and the outer tube, and the fourth opening communicates with the inside of the outer tube, and the plasma preparation device further comprises a first plug matched with the third opening and a second plug matched with the fourth opening.

[0035] In this way, the plasma preparation device is only temporarily connected to the outside world when blood is injected or PPP is recovered, thereby reducing contact with external substances and preventing blood from being contaminated.

[0036] In some embodiments, the plasma preparation device further comprises a flow guide pipe, one end of which is connected to the third opening and the other end of which extends to the throat.

[0037] In another aspect of the present application, a method for preparing red blood cell-poor PRP is also provided, which comprises the following steps:

[0038] The blood is loaded into the plasma preparation device described above.

[0039] The plasma preparation device is centrifuged using a centrifuge to obtain supernatant and a precipitate portion.

[0040] The position of the first screw cap is adjusted so that the supernatant flows out of the second assembly through the adjusting portion and the first opening; and

[0041] The red blood cell-poor PRP is extracted from the second assembly through the extraction assembly.

[0042] In this way, the red blood cell-poor PRP can be prepared by using the red blood cell-poor PRP preparation device.

[0043] In some embodiments, the following step is further included: recovering the PPP flowing out of the inner cavity of the red blood cell-poor PRP preparation device from the outer cavity of the red blood cell-poor PRP preparation device through the sampling device.

[0044] In this way, the PPP separated in the process of preparing the red blood cell-poor PRP by using the red blood cell-poor PRP preparation device can be recovered.

[0045] The details of one or more embodiments of the present application are presented in the following drawings and description. Other features, objects, and advantages of the present application will become apparent from the description, drawings, and claims. BRIEF DESCRIPTION OF DRAWINGS

[0046] In order to more clearly illustrate the technical solutions in the embodiments of the present application or in the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can also be obtained by those skilled in the art without creative labor on the basis of these drawings.

[0047] FIG. 1 is a structural schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0048] Figure 2 is a sectional view of the plasma preparation device shown in Figure 1.

[0049] Figure 3 is a structural schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0050] Figure 4 is a structural schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0051] Figure 5 is an exploded view of the plasma preparation device shown in Figure 4.

[0052] Figure 6 is an enlarged view of portion A of the plasma preparation device shown in Figure 4.

[0053] Figure 7 is a perspective schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0054] Figure 8 shows an exploded schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0055] Figure 9 shows a sectional schematic diagram of a plasma preparation device according to some embodiments of the present application.

[0056] Figure 10 shows a structural schematic diagram of a first rotary cap and an inner cavity connection of a plasma preparation device according to some embodiments of the present application.

[0057] Figure 11 shows a sectional schematic diagram of a first rotary cap and a draw assembly connection of a plasma preparation device according to some embodiments of the present application.

[0058] Figure 12 shows a perspective schematic diagram of a leukocyte filtration structure according to some embodiments of the present application.

[0059] Figure 13 shows an exploded schematic diagram of a pressure head of a plasma preparation device according to some embodiments of the present application.

[0060] 100, plasma preparation device; 10, cover part; 11, top cover sealing film; 12, top cover; 20, first assembly; 21, first screw cover; 211, air hole; 212, filter structure; 22, flow guide tube; 23, extraction assembly; 231, syringe; 232, built-in channel switch; 2321, pressure head; 23211, liquid outlet filter head; 232110, liquid outlet; 232111, liquid outlet clamping plate; 232112, liquid outlet tube; 23212, white blood cell filter structure; 23210, filter membrane layer; 232101, first filter membrane layer; 232102, second filter membrane layer; 23211, through hole; 23213, liquid inlet filter head; 232130, liquid inlet; 232131, liquid inlet clamping plate; 232132, liquid inlet tube; 2322, anti-pollution block; 2323, sealing ring; 24, third opening; 241, first plug cover; 25, fourth opening; 251, second plug cover; 26, first opening; 30, second assembly; 31, first part; 311, adjusting part; 3110, second opening; 32, throat part; 33, second part; 34, first cavity; 40, outer tube; 41, outer tube cavity; 42, sealing structure; 50, sealing member; 51, sealing piece; 511, annular protrusion; 512, clamping groove; 52, second screw cover; 53, connecting plate; 54, clamping block; 200, sampling device. DETAILED DESCRIPTION

[0061] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of protection of the present application.

[0062] It should be noted that when a component is referred to as being "fixed to" or "disposed on" another component, it can be directly on the other component or there can be a middle component. When a component is referred to as being "connected to" another component, it can be directly connected to the other component or there can be a middle component. The terms "vertical", "horizontal", "upper", "lower", "left", "right", and similar expressions used in the specification of the present application are for the purpose of illustration only, and do not represent the only implementation.

[0063] In the present application, unless specifically defined and limited otherwise, the first feature "on", "under", "below", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0064] In the description of the present application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0065] In the description of the present application, it should be noted that, unless otherwise specifically defined and limited, the terms "mounting", "connecting", "connecting" should be broadly understood, for example, it can be fixedly connected, or it can be detachably connected, or integrally connected; it can be mechanically connected, or it can be electrically connected; it can be directly connected, or it can be indirectly connected through an intermediate medium, or it can be connected inside two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0066] In addition, the terms "horizontal", "vertical", "overhanging" and the like do not mean that the parts must be absolutely horizontal or overhanging, but can be slightly inclined. For example, "horizontal" only means that its direction is more horizontal relative to "vertical", and does not mean that the structure must be completely horizontal, but can be slightly inclined.

[0067] Unless otherwise defined, all technical and scientific terms used in the specification of the present application have the same meaning as understood by those skilled in the art to which the present application belongs. The terms used in the specification of the present application are only for the purpose of describing the specific embodiments, and are not intended to limit the present application. The term "and / or" used in the specification of the present application includes any and all combinations of one or more related listed items.

[0068] The concentration of platelet-rich plasma (PRP) is different, and the environment is also different. The existing red blood cell-poor PRP preparation device extracts the middle layer of PRP layer and estimates and extracts part of the upper layer of PPP layer according to the target concentration multiple. Although the existing red blood cell-poor PRP preparation device can prepare red blood cell-poor PRP, the concentration multiple of the prepared red blood cell-poor PRP has an error with the target concentration multiple.

[0069] In the related art, the PRP preparation device extracts the middle layer of PRP layer and estimates and extracts part of the upper layer of PPP layer according to the target concentration multiple. Although the existing red blood cell-poor PRP preparation device can prepare red blood cell-poor PRP, the PPP layer and the PRP layer are mixed during extraction, and the concentration multiple of the prepared red blood cell-poor PRP has an error with the target concentration multiple. In order to solve this problem, the present application provides a plasma preparation device and method which can prevent the mixing of PPP layer and PRP layer during extraction.

[0070] In one aspect of the present application, a plasma preparation device 100 is provided. The plasma preparation device 100 comprises: a first assembly 20, a first rotating cap 21 arranged at one end of the first assembly 20, a second assembly 30, a first part 31 and a second part 33, the first part 31 being rotatably sleeved on the first assembly 20, and the second part 33 being communicated with the first part 31, wherein an adjusting part 311 is arranged on the side wall of the first part 31, a first opening 26 is arranged on the side wall of the first assembly 20, and the first rotating cap 21 is arranged to drive the first assembly 20 to rotate synchronously to adjust the position between the first opening 26 and the adjusting part 311; an outer tube 40 which is liquid-tightly sleeved on the second assembly 30; a sealing member 50 which is liquid-tightly arranged below the second assembly 30, and the sealing member 50 is at least partially movable up and down along the axis direction of the plasma preparation device 100.

[0071] In the present application, when the sealing member 50 moves upward, the volume (i.e. the actual volume capable of containing liquid) in the second assembly 30 can be reduced, and when the sealing member 50 moves downward, the volume in the second assembly 30 can be increased.

[0072] In the embodiment, the inner wall of the first part 31 of the second assembly 30, the inner wall of the first assembly 20 and the inner wall of the second part 33 of the second assembly 30 enclose a first cavity, blood and anticoagulant are injected into the first cavity, the plasma preparation device containing blood and anticoagulant is placed in a centrifuge for centrifugation, after centrifugation, the blood is separated into a platelet-poor plasma layer (PPP layer), a platelet-rich plasma layer (PRP layer) and a red blood cell layer along the height direction of the plasma preparation device from top to bottom (i.e. from the direction in which the first rotary cover 21 points to the second assembly 30); the sealing member 50 is driven to move upward or downward (the direction close to the first rotary cover 21 is upward) to move the separated blood upward or downward until the PPP layer is located in the first part 31 of the second assembly 30; the first rotary cover 21 is driven to move, the first rotary cover 21 drives the first assembly 20 to move to open the adjusting part 311, thereby opening the channel through which the PPP enters the outer lumen 41 from the first part 31 of the second assembly 30, the PPP in the first part 31 of the second assembly 30 can overflow into the outer lumen 41 through the adjusting part 311; when the PPP overflows by a set amount, the first rotary cover 21 is driven to move, the first rotary cover 21 drives the first assembly 20 to move to close the adjusting part 311, thereby closing the channel to prevent the PPP from overflowing into the outer lumen 41; the remaining PPP and PRP in the first cavity can be extracted by a syringe or the like to obtain platelet-rich plasma with a desired concentration multiple.

[0073] The plasma preparation device provided in the embodiment can control the opening and closing of the adjusting part 311 through the first assembly 20 to adjust the amount of PPP overflowing into the outer lumen 41, thereby adjusting the remaining amount of PPP in the first cavity, further adjusting the total amount of platelet-rich plasma and platelet-poor plasma, and further adjusting the concentration multiple of the platelet-rich plasma. The plasma preparation device provided in the embodiment can realize adjustable platelet-rich plasma concentration multiple, so that platelet-rich plasma with different concentration multiples can be prepared according to different requirements.

[0074] There are various ways to achieve the opening and closing of the adjusting part 311 by movably arranging the first assembly 20 in the first part 31 of the second assembly 30, for example, the first assembly 20 is slidingly arranged in the first part 31 of the second assembly 30, that is, the first assembly 20 can move up and down along the height direction of the plasma preparation device 100 to expose the adjusting part 311 (i.e. open the adjusting part 311) or shield the adjusting part 311 (i.e. close the adjusting part 311).

[0075] As an optional solution, the first assembly 20 is rotatably arranged in the first part 31 of the second assembly 30 to control the opening and closing of the adjusting part 311, so that the structure of the plasma preparation device can be compact and occupy less space during use.

[0076] The first assembly 20 can be in the shape of an arc plate. By rotating the first assembly 20, the adjusting part 311 can be exposed (i.e., opened) or covered (i.e., closed).

[0077] As an optional solution, the first assembly 20 is rotatably arranged in the first part 31 of the second assembly 30. The first assembly 20 is provided with a first opening 26 which can be in communication with the adjusting part 311.

[0078] In the embodiment, when the first opening 26 of the first assembly 20 is in communication with the adjusting part 311 of the first part 31 of the second assembly 30, the adjusting part 311 is opened. Otherwise, the adjusting part 311 is closed. The first assembly 20 can be in the shape of a cylinder. The first assembly 20 can be positioned in the first part 31 of the second assembly 30, so that the rotation of the first assembly 20 is stable.

[0079] Specifically, in the circumferential direction of the first part 31 of the second assembly 30, the first part 31 of the second assembly 30 is provided with at least one adjusting part 311, and the first assembly 20 is provided with at least one first opening 26.

[0080] As an optional solution, the adjusting part 311 is a second opening 3110. The number of the second openings 3110 and the number of the first openings 26 are both one. The overflow amount of the PPP can be adjusted by controlling the communication time of the second opening 3110 and the first opening 26, so as to adjust the remaining amount of the PPP in the first cavity, and further adjust the concentration multiple of the platelet-rich plasma.

[0081] As an optional solution, the number of the second openings 3110 is multiple, and the number of the first openings 26 is multiple. The multiple second openings 3110 and the multiple first openings 26 are one-to-one corresponding, i.e., the second opening 3110 and the first opening 26 are arranged in pairs. When one pair of the second opening 3110 and the first opening 26 are in communication, the rest of the pairs of the second opening 3110 and the first opening 26 are also in communication. The overflow amount of the PPP can be adjusted by controlling the communication time of the second opening 3110 and the first opening 26, so as to adjust the remaining amount of the PPP in the first cavity, and further adjust the concentration multiple of the platelet-rich plasma.

[0082] In order to facilitate use, a scale line can be arranged on the outer wall of the first part 31 of the second assembly 30 to indicate the sum of the remaining amount of the PPP and the amount of the PRP after overflow in the first cavity 34, that is, at which scale line the liquid surface drops to, which indicates that the sum of the remaining amount of the PPP and the amount of the PRP is the amount of the corresponding scale line. For example, the scale line 0-10 ml is arranged on the outer wall of the first part 31 of the second assembly 30, the blood amount injected is 30 ml, and the liquid surface in the first cavity 34 finally stays at 5 ml, so that the platelet-rich plasma with a concentration multiple of 6 times is obtained (concentration multiple=(V1÷V2)×DF; wherein V1 represents the volume of the original blood, in milliliter; V2 represents the volume of the platelet-rich plasma obtained after concentration, in milliliter; and DF represents the dilution multiple, which is 1 if no dilution is performed); the blood amount injected is 30 ml, and the liquid surface in the first cavity 34 finally stays at 6 ml, so that the platelet-rich plasma with a concentration multiple of 5 times is obtained; the blood amount injected is 40 ml, and the liquid surface in the first cavity 34 finally stays at 5 ml, so that the platelet-rich plasma with a concentration multiple of 8 times is obtained.

[0083] As an optional solution, the number of the second openings 3110 is multiple, and the multiple second openings 3110 are arranged in a stepped manner on the peripheral wall of the first part 31 of the second assembly 30; the number of the first opening 26 is one, and the first opening 26 extends along the height direction of the plasma preparation device 100. Specifically, the cross-sectional area of the first opening 26 is not less than the sum of the cross-sectional areas of the multiple second openings 3110, and the first opening 26 can cover each second opening 3110.

[0084] In the embodiment, when the first opening 26 is opposite to one second opening 3110, the shape of the cross section of the channel formed is the same as the shape of the second opening 3110. By rotating the first assembly 20, the position of the first opening 26 can be changed, so that the position of the channel is changed, and the bottom of the channel, that is, the bottom end of the corresponding second opening 3110 (the end close to the second part 33 of the second assembly 30, if the second opening 3110 has a bottom edge, the bottom end is the bottom edge) is the overflow cutoff. When the first opening 26 stops at one second opening 3110, the PPP above the bottom end of the second opening 3110 overflows to the outer cavity 41.

[0085] The second openings 3110 can be marked with scales to indicate the remaining amount of plasma in the first cavity 34. For example, if there are five second openings 3110, the five second openings 3110 from bottom to top can correspond to the scales of 2 mL, 4 mL, 6 mL, 8 mL, and 10 mL, respectively. If the amount of blood injected is 20 mL, the five second openings 3110 from bottom to top can correspond to the scales of 10-fold, 5-fold, 3.3-fold, 2.5-fold, and 2-fold, respectively. If the amount of blood injected is 30 mL, the four second openings 3110 can correspond to the scales of 7.5-fold, 5-fold, 3.75-fold, and 3-fold, respectively.

[0086] The second openings 3110 can be triangular, quadrilateral, pentagonal, elliptical, or circular, or the like. Alternatively, the plurality of second openings 3110 can have the same shape, which is convenient for processing.

[0087] In some embodiments, the number of second openings 3110 is one, the shape of the second opening 3110 is triangular, and the first opening 26 is arranged along the height direction of the plasma preparation device. The upper edge of the second opening 3110 is flush with the upper edge of the first part 31.

[0088] The cross-sectional areas of the plurality of second openings 3110 can be the same or different. For example, the cross-sectional areas of the plurality of second openings 3110 can gradually increase from bottom to top or from top to bottom.

[0089] In some embodiments, as shown in FIGS. 4-6, the number of second openings 3110 is one, and the second opening 3110 spirally rises along the peripheral wall of the first part 31 of the second assembly 30 from bottom to top. The number of first openings 26 is one, and the first opening 26 extends along the height direction of the plasma preparation device 100.

[0090] In this embodiment, the plurality of second openings 3110 spirally wind around the outer peripheral wall of the first part 31 of the second assembly 30 (the first part 31 of the second assembly 30 is arranged in a cylindrical shape). The channel is surrounded by the upper and lower edges of the second opening 3110 and the left and right edges of the first opening 26, and the lower end of the channel is the overflow cutoff. By rotating the first assembly 20, the position of the first opening 26 relative to the second opening 3110 is changed, thereby changing the height of the bottom end of the channel, and further changing the height of the liquid level, thereby adjusting the overflow amount of PPP, and further adjusting the concentration multiple of platelet-rich plasma.

[0091] The scale lines can be arranged on the outer peripheral wall of the first part 31 of the second assembly 30 at different heights of the second opening 3110 to indicate the remaining amount of the plasma in the first cavity 34, for example, 2ml, 4ml, 6ml, 8ml, 10ml scale lines are arranged from bottom to top. When the injected blood volume is 30ml, the bottom end of the channel is at the 10ml scale line, and the platelet-rich plasma with a concentration of 3 times is obtained. When the injected blood volume is 30ml, the bottom end of the channel is at the 5ml scale line, and the platelet-rich plasma with a concentration of 6 times is obtained. When the injected blood volume is 30ml, the bottom end of the channel is at the 3ml scale line, and the platelet-rich plasma with a concentration of 10 times is obtained.

[0092] It is easy to understand that at least part of the outer tube 40 (i.e., the outer wall of the plasma preparation device 100) is made of a transparent material (for example, plastic or glass, etc.), and the staff can observe the change of the liquid level in the first cavity 34 and the corresponding scale line.

[0093] On the basis of the above embodiment, further, the plasma preparation device 100 further comprises a throat part 32 and an outer tube 40; the throat part 32 is communicated between the second part 33 of the second assembly 30 and the first part 31 of the second assembly 30 (in the height direction of the main tube 1, from top to bottom, the first part 31 of the second assembly 30, the throat part 32 and the second part 33 of the second assembly 30 are sequentially arranged); the outer tube 40 is sleeved outside the first part 31 of the second assembly 30 and the throat part 32 to form an outer tube cavity 41; and the first screw cap 21 is connected with the upper end of the outer tube 40.

[0094] In the embodiment, it can be understood that the cross-sectional area of the throat part 32 is smaller than the cross-sectional area of the first part 31 of the second assembly 30 and the cross-sectional area of the second part 33 of the second assembly 30. The layered blood can be moved upward or downward by driving the sealing member 50 to move upward or downward until the PRP layer is located in the throat part 32, the red blood cell layer is located in the second part 33 of the second assembly 30, and the PPP layer is located in the first part 31 of the second assembly 30, which is more convenient for extracting the required liquid.

[0095] The second assembly 30 can be integrally formed.

[0096] Alternatively, the second assembly 30 is formed by assembly, wherein the first part 31 of the second assembly 30, the throat part 32 and the second part 33 of the second assembly 30 are integrally formed. One end of the outer tube 40 is connected with the outer wall of the second part 33 of the second assembly 30, and there is a gap between the inner wall of the outer tube 40 and the outer wall of the first part 31 of the second assembly 30 and between the outer tube 40 and the outer wall of the throat part 32. The outer tube 40, the throat part 32 and the first part 31 of the second assembly 30 form the outer tube cavity 41. The upper end of the outer tube 40 is connected with the first screw cap 21.

[0097] A sealing structure 42 (for example, a sealing filler layer, a sealing ring, etc.) can be arranged between the outer tube 40 and the first screw cap 21 to improve the sealing performance of the connection between the outer tube 40 and the first screw cap 21.

[0098] On the basis of the above-mentioned embodiments, the volume of the first part 31 of the second assembly 30, the throat part 32, and the second part 33 of the second assembly 30 can be set as required.

[0099] Preferably, the ratio of the sum of the volume of the cavity formed by the inner wall of the first assembly 20 and the inner wall of the first part 31 of the second assembly 30 to the volume of the throat part 32 to the volume of the second part 33 of the second assembly 30 is 1:1.2 (for example, 1:1, 1:1.1, or 1:1.2, etc.), which is beneficial to the distribution of the PPP layer, the PRP layer, and the red blood cell layer in different cavities.

[0100] On the basis of the above-mentioned embodiments, the sealing member 50 can have various structural forms, for example, the sealing member 50 includes a push block and a push-pull rod, the push block is slidingly arranged in the second part 33 of the second assembly 30, and the push-pull rod is connected to the end of the push block away from the throat part 32, so that the push block can be moved in the second part 33 of the second assembly 30 by the push-pull rod.

[0101] As an optional solution, as shown in FIG. 2 and FIG. 5, the sealing member 50 includes a sealing piece 51 and a second screw cap 52, the outer wall of the sealing piece 51 is provided with a plurality of annular protrusions 511 at intervals in the height direction of the plasma preparation device 100, the annular protrusions 511 abut against the inner wall of the second part 33 of the second assembly 30, the second screw cap 52 is connected to the sealing piece 51, and the second screw cap 52 is threadedly connected to the second part 33 of the second assembly 30.

[0102] In this embodiment, a groove is formed between two adjacent annular protrusions 511, and the plurality of annular protrusions 511 and the plurality of grooves are combined to form a labyrinth seal, thereby improving the sealing performance between the sealing piece 51 and the second part 33 of the second assembly 30. The second screw cap 52 is threadedly connected to the second part 33 of the second assembly 30, so that the position of the second screw cap 52 relative to the second part 33 of the second assembly 30 can be changed by rotating the second screw cap 52, and the second screw cap 52 can move up and down in the height direction of the second part 33 of the second assembly 30, thereby realizing the up-and-down movement of the sealing piece 51 in the second part 33 of the second assembly 30 to change the position of the liquid level in the inner cavity. The sealing member 50 of this structure has the advantages of compact structure, small space occupation, reliable connection, and smooth movement.

[0103] The second screw cap 52 can be connected to the sealing piece 51 by a fastener such as a bolt or a screw.

[0104] As an alternative, as shown in FIG. 2 and FIG. 5, an outer wall of the lower part of the sealing member 51 is provided with a clamping groove 512; the sealing member 50 further comprises a connecting plate 53 and a clamping block 54; the connecting plate 53 is fixed at the bottom of the second screw cap 52; the clamping block 54 is fixed with the connecting plate 53, and the clamping block 54 is clamped in the clamping groove 512; an insertion slot is formed between the inner wall of the second screw cap 52, the connecting plate 53 and the clamping block 54, and the bottom of the second part 33 of the second assembly 30 is provided with an opening, and the lower end of the second part 33 of the second assembly 30 is inserted into the insertion slot.

[0105] In this embodiment, one side of the connecting plate 53 is connected with the bottom of the second screw cap 52, and the other side is connected with the clamping block 54, so that a space is formed between the second screw cap 52 and the clamping block 54, thereby allowing the bottom of the second part 33 of the second assembly 30 to be inserted. The connection mode of the second screw cap 52 and the sealing member 51 has a simple structure, is convenient to install, and has a regular and compact structure.

[0106] As shown in FIG. 2, the first screw cap 21 is provided with a third opening 24; the blood plasma preparation device further comprises a flow guide pipe 22, one end of the flow guide pipe 22 is connected with the third opening 24, and the other end extends to the throat part 32. The flow guide pipe 22 is provided to facilitate the suction of the liquid in the first cavity 34.

[0107] As shown in FIG. 1 and FIG. 2, on the basis of the above-mentioned embodiments, further, the first screw cap 21 is provided with a ventilation hole 211, and a filter structure 212 is arranged at the ventilation hole 211; the first cavity 34 and the outer pipe cavity 41 surrounded by the first part 31 of the second assembly 30 and the first assembly 20 are in communication with the ventilation hole 211.

[0108] In this embodiment, the first cavity 34 and the outer pipe cavity 41 can be in communication with the external environment through the ventilation hole 211, thereby achieving internal and external pressure balance; the filter structure 212 is arranged to prevent bacteria from entering the first cavity 34 and the outer pipe cavity 41.

[0109] As shown in FIG. 1 and FIG. 2, on the basis of the above-mentioned embodiments, further, the first screw cap 21 is further provided with a fourth opening 25 in communication with the outer pipe cavity 41, the PPP in the outer pipe cavity 41 can be sucked out through the fourth opening 25, and a PPP flow guide pipe can be arranged in the outer pipe cavity 41 to facilitate the suction; the blood plasma preparation device further comprises a first plug cap 241 and a second plug cap 251, the first plug cap 241 is installed at the third opening 24, and the second plug cap 251 is installed outside the fourth opening 25, thereby improving the sealing performance of the entire device.

[0110] Please refer to FIG. 7 to FIG. 8, one embodiment of the present application provides a blood plasma preparation device 100, which can comprise a second assembly 30 for containing blood and a suction assembly 23 for extracting PPP layer and PRP layer.

[0111] As shown in FIG. 7 to FIG. 7, in some embodiments, when the first rotating cover 21 rotates relative to the second assembly 30, the intersection between the first opening 26 and the adjusting portion 311 moves up and down relative to the plasma preparation device 100; the extraction assembly 23 is detachably connected to the first rotating cover 21 for extracting PRP in the second assembly 30. The adjusting portion 311 is a second opening 3110.

[0112] It is worth noting that, compared with the existing direct extraction scheme, the present scheme can prevent the mixing of the PPP layer and the PRP layer during extraction to affect the concentration of the prepared red blood cell-poor PRP by rotating the first rotating cover 21 relative to the second assembly 30 to move the intersection between the first opening 26 and the second opening 3110 up and down relative to the plasma preparation device 100, thereby separating part of the PPP.

[0113] In some embodiments, as shown in FIG. 10, the first opening 26 is a straight slot extending straight on the peripheral wall of the first rotating cover 21, and the second opening 3110 is a spiral slot extending curvedly on the peripheral wall of the first portion 31.

[0114] It is worth noting that in this way, the angle of rotating the first rotating cover 21 relative to the second assembly 30 is linearly related to the distance of the intersection moving up and down, so as to accurately separate the part of the PPP and reduce the error.

[0115] In some embodiments, as shown in FIG. 10, the central angle of the spiral slot is less than 180°, in other words, the spiral slot does not surround the first portion 31 for one turn, that is, there is no intersection between the first opening 26 and the second opening 3110.

[0116] It is worth noting that the non-intersection between the first opening 26 and the second opening 3110 can prevent the blood from leaking when the plasma preparation device 100 is placed in the centrifuge for extraction.

[0117] In some embodiments, as shown in FIG. 8 and FIG. 9, the plasma preparation device 100 further comprises an outer tube 40 detachably connected to the first rotating cover 21 and the second assembly 30, one end of the outer tube 40 is sealingly connected to the first rotating cover 21, and the other end of the outer tube 40 is sealingly connected to the second assembly 30 away from the end connected to the first rotating cover 21, to form an outer tube cavity 41.

[0118] It is worth noting that the first screw cap 21 is provided with a scale related to the concentration multiple and a number corresponding to the scale (concentration multiple), and the outer tube 40 is also provided with a mark corresponding to the scale provided on the first screw cap 21 (the outer tube 40 is fixedly connected with the second assembly 30, and the relative connection position of the outer tube 40 and the second assembly 30 is unique). The position of the mark and the scale is related to the distance of the intersection between the first opening 26 and the second opening 3110 moving up and down relative to the plasma preparation device 100 when the first screw cap 21 rotates by a unit angle relative to the outer tube 40, that is, when the first screw cap 21 is rotated relative to the outer tube 40 to make the mark rotate to a certain scale, the red blood cell-poor PRP with the concentration multiple corresponding to the scale can be prepared.

[0119] It is worth noting that the outer tube cavity 41 is used to accommodate the part of PPP to be separated, so as to recover the PPP.

[0120] In some embodiments, as shown in FIGS. 8 and 9, the plasma preparation device 100 further comprises a sealing member 51 sealingly connected to the outer tube 40, and the sealing member 51, the outer tube 40, the first screw cap 21 and the second assembly 30 jointly form a first cavity 34.

[0121] It is worth noting that the first cavity 34 can be used to accommodate blood, and after the red blood cell-poor PRP is prepared by means of the plasma preparation device 100, the sealing member 51 can be detached to facilitate cleaning of the plasma preparation device 100.

[0122] In some embodiments, as shown in FIGS. 8 and 9, the first screw cap 21 has a flow guide tube 22 for accommodating the extraction assembly 23, and the extraction assembly 23 is inserted into the flow guide tube 22.

[0123] Particularly, as shown in FIG. 8 and FIG. 11, the extraction assembly 23 comprises a syringe 231 and an internal channel switch 232 connected to the end of the syringe 231 away from the first cap 21 and movable up and down with the syringe 231. The internal channel switch 232 comprises a sealing ring 2323, a pressing head 2321 and an anti-fouling block 2322, which can be partially or wholly integrated on the internal channel switch 232. The sealing ring 2323 is sealingly connected to the inner wall of the flow guide tube 22, the pressing head 2321 is movable up and down in the flow guide tube 22, and the anti-fouling block 2322 is in contact with the flow guide tube 22 to block the port at the end of the flow guide tube 22 away from the first cap 21, thereby isolating the blood material in the first cavity 34 from entering the flow guide tube 22. By moving or rotating the syringe 231, the pressing head 2321 in the internal channel switch 232 is displaced, thereby destroying or moving the anti-fouling block 2322, so that the anti-fouling block 2322 fails to function and cannot continue to isolate the blood material in the first cavity 34 from entering the flow guide tube 22. Pulling the push rod at the tail of the syringe 231, the syringe 231 can smoothly extract the PRP layer and the PPP layer in the first cavity 34 due to the hydraulic effect.

[0124] Particularly, as shown in FIG. 13, the pressing head 2321 comprises a liquid outlet filter head 23211 with a liquid outlet 232110, a liquid inlet filter head 23213 with a liquid inlet 232130, and a white blood cell filtering structure 23212 disposed between the liquid outlet filter head 23211 and the liquid inlet filter head 23213. The white blood cell filtering structure 23212 comprises a plurality of filter membrane layers 23210 stacked on each other, at least one of which has one or more through holes 23211, and the through holes 23211 on different filter membrane layers 23210 are misaligned with each other, and the diameter of the through holes 23211 is greater than the diameter of white blood cells.

[0125] Notably, the internal channel switch 232 can filter the white blood cells in the red blood cell-removed and white blood cell-depleted PRP prepared by the plasma preparation device 100 through the white blood cell filtering structure 23212.

[0126] Particularly, as shown in FIG. 13, the liquid outlet filter head 23211 comprises a liquid outlet clamping plate 232111 connected to the white blood cell filtering structure 23212 and a liquid outlet tube 232112 protruding from the liquid outlet clamping plate 232111, and the liquid inlet filter head 23213 comprises a liquid inlet clamping plate 232131 connected to the white blood cell filtering structure 23212 and a liquid inlet tube 232132 protruding from the liquid inlet clamping plate 232131, and the anti-fouling block 2322 abuts against the liquid inlet tube 232132 to block the port of the liquid inlet tube 232132.

[0127] In particular, as shown in FIGS. 12 and 13, the leukocyte filter structure 23212 includes a plurality of filter membrane layers 23210 stacked on each other, at least one of the filter membrane layers 23210 having one or more through-holes 23211, and the through-holes 23211 on different filter membrane layers 23210 being misaligned with each other, the through-holes 23211 having a diameter greater than that of a leukocyte.

[0128] Notably, in this way, the leukocyte filter structure 23212 can have the advantages of filtering a large amount of leukocytes and adsorbing a small amount of platelets and plasma. The filter membrane layers 23210 having the through-holes 23211 can filter leukocytes faster than the filter membrane layers 23210 without the through-holes 23211, thereby improving the passability of the leukocyte filter structure 23212 and reducing the amount of platelets and plasma remaining in the filter membrane layers 23210. The leukocyte filter structure 23212 with a plurality of filter membrane layers 23210 can perform multiple filtrations, thereby increasing the amount of leukocytes filtered. Thus, the leukocyte filter structure 23212 can have the advantages of filtering a large amount of leukocytes and adsorbing a small amount of platelets and plasma.

[0129] Illustratively, as shown in FIGS. 12 and 13, the plurality of filter membrane layers 23210 includes a first filter membrane layer 232101 without the through-holes 23211 and one or more second filter membrane layers 232102 stacked on one side of the first filter membrane layer 232101 and having the through-holes 23211.

[0130] Notably, in this way, the first filter membrane layer 232101 without the through-holes 23211 can perform a total filtration on P-PRP (leukocyte-poor platelet-rich plasma) obtained by filtration through the plurality of second filter membrane layers 232102, preventing a small number of leukocytes from passing through the gaps between the first filter membrane layer 232101 and the second filter membrane layers 232102 and between the second filter membrane layers 232102, continuously passing through the through-holes 23211 of the second filter membrane layers 232102, and remaining in the P-PRP (leukocyte-poor platelet-rich plasma), thereby increasing the amount of leukocytes filtered.

[0131] Notably, the first filter membrane layer 232101 can be disposed between the plurality of second filter membrane layers 232102, i.e., the filtration of PRP (platelet-rich plasma) by the first filter membrane layer 232101 is advanced from the last to the middle in the order of filtration.

[0132] As shown in FIG. 13, the first filter membrane layer 232101 and the second filter membrane layer 232102 each has a diameter less than 5 cm. In some embodiments, the first filter membrane layer 232101 and the second filter membrane layer 232102 each has a diameter less than 3 cm. Compared with a larger size single-layer filter membrane with the same filtration rate, the overall size of the leukocyte filtration structure 23212 is smaller, and compared with a multi-layer filter membrane with the same filtration efficiency, the filtration rate of the leukocyte filtration structure 23212 is faster, and the leukocyte filtration structure 23212 has both fast filtration rate and is suitable for high size requirement.

[0133] As shown in FIG. 13, the second filter membrane layer 232102 has a number of through holes 23211 ranging from 1 to 10. In some embodiments, the second filter membrane layer 232102 has a number of through holes 23211 ranging from 1 to 4. In this way, by setting a reasonable number of through holes 23211, the occurrence of overlapping between the through holes 23211 of two adjacent second filter membrane layers 232102 can be prevented, and at the same time, the occurrence of overlapping between two through holes 23211 of two second filter membrane layers 232102 separated by one second filter membrane layer 232102 can be reduced, thereby ensuring the leukocyte filtration effect of the leukocyte filtration structure 23212.

[0134] As shown in FIG. 13, the second filter membrane layer 232102 has a diameter of the through hole 23211 ranging from 0.5 to 10 mm. In some embodiments, the second filter membrane layer 232102 has a diameter of the through hole 23211 ranging from 0.5 to 5 mm. In this way, by setting a reasonable number of through holes 23211, the occurrence of overlapping between the through holes 23211 of two adjacent second filter membrane layers 232102 can be prevented, and at the same time, the occurrence of overlapping between two through holes 23211 of two second filter membrane layers 232102 separated by one second filter membrane layer 232102 can be reduced, thereby ensuring the leukocyte filtration effect of the leukocyte filtration structure 23212.

[0135] As shown in FIG. 13, the second filter membrane layer 232102 has a plurality of through holes 23211 uniformly distributed therein. Compared with the position on the second filter membrane layer 232102 where no through hole 23211 is provided, the flow rate at the through hole 23211 is faster. By uniformly providing a plurality of through holes 23211 in the second filter membrane layer 232102, the filtration rate of each position of the second filter membrane layer 232102 during leukocyte filtration can be controlled to be relatively average, thereby improving the filtration effect of the leukocyte filtration structure 23212.

[0136] As shown in FIG. 13, the second filter membrane layer 232102 has the same diameter of the through holes 23211, and the flow rate at the through holes 23211 with different diameters is different, and the flow rate at the through holes 23211 with larger diameter is greater. The second filter membrane layer 232102 is provided with the through holes 23211 with the same diameter, which can ensure that the efficiency of the second filter membrane layer 232102 in filtering white blood cells is relatively average, thereby improving the filtering effect of the white blood cell filtering structure 23212.

[0137] As shown in FIG. 13, the closer to the first filter membrane layer 232101, the smaller the diameter of the through holes 23211 on the second filter membrane layer 232102. The larger the diameter of the through holes 23211 of the second filter membrane layer 232102, the faster the rate of filtering white blood cells, but the filtering effect is relatively reduced. The PRP (platelet-rich plasma) can be first coarsely filtered at a faster rate and then gradually improve the effect of filtering white blood cells in the process of filtering white blood cells by means of the white blood cell filtering structure 23212, thereby making the white blood cell filtering structure 23212 have the advantages of fast rate and good filtering effect in the process of filtering white blood cells.

[0138] As shown in FIG. 13, the number of layers of the filter membrane layer 23210 in the white blood cell filtering structure 23212 is 2, including 1 layer of the first filter membrane layer 232101 and 1 layer of the second filter membrane layer 232102, and the diameter of each filter membrane layer 23210 is 25 mm. The second filter membrane layer 232102 has 2 through holes 23211, and the diameter of the through holes 23211 of the second filter membrane layer 232102 is 2 mm. The through holes 23211 of the second filter membrane layer 232102 are uniformly distributed in the second filter membrane layer 232102.

[0139] In some embodiments, the diameters of the through holes 23211 of two adjacent second filter membrane layers 232102 can be the same.

[0140] In some embodiments, as shown in FIGS. 8 and 9, the second assembly 30 has a through space penetrating through the second assembly 30, and the through space has a throat 32 with a smaller cross section than the cross section of the second assembly 30.

[0141] In some embodiments, the through space is a first cavity 34.

[0142] It is worth noting that, compared with the scheme without the throat 32, the scheme with the throat 32 reduces the mutual mixing of the liquids contained in the first cavity 34 due to vibration when the PRP layer and the PPP layer are extracted by means of the extraction assembly 23, thereby affecting the concentration multiple of the finally obtained PRP.

[0143] In some embodiments, as shown in FIG. 8, the sealing member 50 further comprises a second screw cap 52, which is threadedly connected to the outer tube 40 and carries the sealing member 51.

[0144] It is worth noting that in this way, the position of the sealing member 51 and the second screw cap 52 relative to the outer tube 40 can be adjusted by means of threads using the second screw cap 52, thereby adjusting the liquid level of the liquid contained in the first cavity 34 relative to the cavity 30, and thereby preventing the PRP layer from being completely extracted by the extraction assembly 23 due to insufficient volume of the liquid contained in the first cavity 34 when the PRP layer and the PPP layer are extracted by means of the extraction assembly 23. Ultimately, the situation of insufficient platelet content in the prepared red blood cell-depleted PRP occurs.

[0145] In some embodiments, as shown in FIG. 8, the first screw cap 21 further has a third opening 24 and a fourth opening 25, and the plasma preparation device 100 further comprises a first plug cap 241 matched with the third opening 24 and a second plug cap 251 matched with the fourth opening 25.

[0146] It is worth noting that in this way, the plasma preparation device 100 is only temporarily connected to the outside world when injecting blood or recovering PPP, thereby reducing contact with external substances and preventing blood from being contaminated.

[0147] In particular, as shown in FIG. 8, the first screw cap 21 further comprises a through vent hole 211, which is provided with a filter structure 212, thereby preventing bacteria from entering the first screw cap 21 while balancing the air pressure of the plasma preparation device 100.

[0148] In particular, as shown in FIG. 7 and FIG. 8, the plasma preparation device 100 further comprises a cover part 10, which is detachably and sealingly connected to the first screw cap 21, for isolating external substances from entering the first screw cap 21, thereby preventing blood from being contaminated. The cover part 10 comprises a top cover sealing film 11 and a top cover 12, which has a hollow structure penetrating through the top cover 12, and the top cover sealing film 11 is detachably arranged at one end of the top cover 12 away from the first screw cap 21. The material of the top cover sealing film 11 includes but is not limited to Tyvek paper, aluminum foil, plastic film and other sealable materials.

[0149] It is worth noting that the plasma preparation device 100 is overall sealed and has high integration, and only temporarily connected to the outside world during the process of injecting blood, extracting the prepared red blood cell-depleted PRP and recovering PPP, thereby reducing the risk of blood being contaminated by external substances.

[0150] Specifically, the method for preparing the red blood cell-poor PRP comprises the following steps:

[0151] S100, loading blood into the plasma preparation device;

[0152] S200, using a centrifuge to centrifuge the plasma preparation device to obtain supernatant and a precipitate portion;

[0153] S300, adjusting the position of the first rotating cover so that the supernatant flows out of the second assembly through the adjusting portion and the first opening; and

[0154] S400, extracting the red blood cell-poor PRP from the second assembly through the extraction assembly.

[0155] In some embodiments, S300 comprises: rotating the plasma preparation device 100 relative to the second assembly 30 of the plasma preparation device 100, moving the intersection between the first opening 26 and the adjusting portion 311 up and down so that part of the PPP flows out of the inner cavity 302 of the red blood cell-poor PRP preparation device 1 through the intersection; and

[0156] S300: extracting the red blood cell-poor PRP from the inner cavity 32 through the extraction assembly 23 of the red blood cell-poor PRP preparation device 1.

[0157] Notably, in this way, the red blood cell-poor PRP can be prepared by means of the plasma preparation device 100.

[0158] Notably, the process of preparing the red blood cell-poor PRP using the method for preparing the red blood cell-poor PRP only involves one centrifugation.

[0159] In particular, in the step of centrifuging the plasma preparation device 100 containing blood using a centrifuge, the parameters set in the centrifuge can be increased to a first rotational speed within 1-120 s and maintained for 0-5 min, then increased to a second rotational speed again within 1-120 s and maintained for 0-5 min, and then reduced to 0. The first and second rotational speeds are 500-3000 g, and if necessary, third, fourth, and nth rotational speeds can be set. For example, the rotational speed is increased to 1000 g within 20 s and maintained for 3 min, then increased to 2000 g within 20 s and maintained for 5 min, and then reduced to 0.

[0160] In particular, the method for preparing the red blood cell-poor PRP further comprises the following steps between the step S100 and the step S200:

[0161] Adjusting the relative position of the second rotating cover 52 and the cavity 30 so that the end of the flow guide tube 22 close to the first cavity 34 is located at a specified position based on the interface between the PRP layer and the red blood cell layer.

[0162] It is worth noting that in this way the extraction assembly 23 can completely extract the PRP layer, ensuring that the prepared leukocyte-depleted PRP contains a high content of platelets.

[0163] In some embodiments, as shown, the leukocyte-depleted PRP preparation method further comprises the following steps:

[0164] S400: Recover the separated part of PPP from the designated concentration plasma preparation device 100 through the sampling device 200.

[0165] It is worth noting that the above-mentioned sampling device 200 is not the same as the syringe 231. Moreover, in this way, the PPP separated during the preparation of leukocyte-depleted PRP by means of the plasma preparation device 100 can be recovered.

[0166] In summary, using the plasma preparation device 100 and the method can prepare the leukocyte-depleted PRP while avoiding the mixing of the PPP layer and the PRP layer caused by the vibration generated during the extraction of the PPP layer and the PRP layer, thereby affecting the concentration of the leukocyte-depleted PRP.

[0167] The technical features of the above embodiments can be combined without changing the basic principles of the present application. In order to make the description concise, not all possible combinations of the technical features in the above embodiments are described, but as long as the combinations of the technical features do not exist contradictory, they should be considered as the scope of the present application.

[0168] The above embodiments only express several implementation manners of the present application, and the description is more specific and detailed, but it should not be understood as a limitation on the patent scope of the application. It should be pointed out that for ordinary skilled in the art, without departing from the concept of the present application, a number of modifications and improvements can be made, which are all within the scope of the present application. Therefore, the patent protection scope of the present application should be subject to the appended claims.

Claims

1. A plasma preparation device, characterized by, Comprise: a first component, one end of the first component is provided with a first rotating cover, a second component, the first part and the second part, the first part is rotatably sleeved on the first component, the second part is communicated with the first part, wherein the side wall of the first part is provided with an adjusting part, the side wall of the first component is provided with a first opening, the first rotating cover can drive the first component to rotate synchronously to adjust the position between the first opening and the adjusting part; an outer tube which liquid-tightly covers the second component; a sealing member which is liquid-tightly arranged below the second component, the sealing member can at least partially move up and down along the axial direction of the plasma preparation device.

2. The plasma preparation device according to claim 1, wherein the adjusting part is at least one second opening, and the at least one second opening is arranged along the circumferential direction of the first part.

3. The plasma preparation device according to claim 2, wherein the number of the second openings is multiple, and the multiple second openings are arranged in a stepped manner on the peripheral wall of the first component; the first opening is arranged along the height direction of the plasma preparation device.

4. The plasma preparation device according to claim 2, wherein the number of the second opening is one, and the shape of the second opening is triangular, the first opening is arranged along the height direction of the plasma preparation device.

5. The plasma preparation device according to claim 1, wherein the adjusting part is one second opening extending along the peripheral wall of the first component, the first opening and the second opening intersect, and when the first rotating cover rotates relative to the second component, the intersection between the first opening and the second opening moves up and down relative to the height direction of the plasma preparation device.

6. The plasma preparation device according to claim 5, wherein the plasma preparation device further comprises an extraction assembly which is detachably connected to the first rotating cover and is used for extracting liquid in the second component.

7. The plasma preparation apparatus of claim 6, wherein, the first opening is a straight slot extending straight on the peripheral wall of the first rotating cover, and the second opening is a spiral slot extending curvedly on the peripheral wall of the first part.

8. The plasma preparation apparatus of claim 7, wherein, The central angle of the spiral slot is less than 180°.

9. The plasma preparation device of any one of claims 1-8, wherein, The second component further comprises: a throat part which is communicated between the first part and the second part, and the cross-sectional area of the throat part is smaller than the cross-sectional area of the first part and the cross-sectional area of the second part respectively.

10. The plasma preparation device of claim 9, wherein, The outer tube is detachably connected to the first rotating cover, and one end of the outer tube is sealingly connected to the first rotating cover.

11. The plasma preparation apparatus of claim 10, wherein, The ratio of the sum of the volume of the cavity surrounded by the first component and the first part to the volume of the second part is 1:1.2-1:

1.

12. The plasma preparation device of any one of claims 1-4, wherein, The sealing member comprises: a sealing part which moves up and down along the axial direction of the plasma preparation device, and a plurality of annular protrusions are arranged on the outer wall of the sealing part and abut against the inner wall of the second part; a second rotating cover which is connected with the sealing part and is connected with the second part through threads.

13. The plasma preparation apparatus of claim 5, wherein, The outer tube is detachably connected to the first screw cap and the second part, one end of the outer tube is sealingly connected to the first screw cap, and the other end of the outer tube is sealingly connected to the second part away from the end connected to the first screw cap.

14. The plasma preparation apparatus of claim 5, wherein, The sealing member comprises a sealing piece and a second screw cap, the second screw cap is threadedly connected to the outer tube, and the second screw cap carries the sealing piece.

15. The plasma preparation device of claim 1, wherein, The first screw cap is provided with a ventilation hole, and the ventilation hole is provided with a filtering structure.

16. The plasma preparation device of claim 1, wherein, The plasma preparation device further comprises an extraction assembly, and the first screw cap is provided with a flow guide tube, and the extraction assembly is inserted into the flow guide tube.

17. The plasma preparation device of claim 16, wherein, The extraction assembly comprises a syringe and an internal channel switch connected to one end of the syringe away from the first screw cap and movable up and down with the syringe, The internal channel switch comprises a pressure head and an anti-fouling block, The pressure head is movable up and down in the flow guide tube, and the anti-fouling block is in contact with the flow guide tube to block the port of the flow guide tube away from the first screw cap.

18. The plasma preparation device according to claim 17, wherein, The pressure head comprises a liquid outlet filter head with a liquid outlet, a liquid inlet filter head with a liquid inlet, and a white blood cell filtering structure arranged between the liquid outlet filter head and the liquid inlet filter head.

19. The plasma preparation device according to claim 12, wherein, The first screw cap is provided with a third opening in communication with the interior of the first assembly, The first screw cap is further provided with a fourth opening, and the fourth opening is arranged between the second assembly and the outer tube, and the fourth opening is in communication with the interior of the outer tube, The plasma preparation device further comprises a first plug matched with the third opening and a second plug matched with the fourth opening.

20. The plasma preparation device according to claim 19, wherein, The plasma preparation device further comprises a flow guide tube, one end of the flow guide tube is connected to the third opening, and the other end of the flow guide tube extends to the throat.

21. A method of preparing red blood cell-poor PRP, characterized by, The method for preparing red blood cell-poor PRP comprises the following steps: Blood is loaded into the plasma preparation device according to any one of claims 1-20; A centrifuge is used to centrifuge the plasma preparation device to obtain supernatant and precipitate; The position of the first screw cap is adjusted so that the supernatant flows out of the second assembly through the adjusting part and the first opening; and Red blood cell-poor PRP is extracted from the second assembly by the extraction assembly.

22. The method of claim 21, wherein the red blood cell-poor PRP is prepared by the method of claim 1. Further comprising the following steps: PPP flowing out of the first cavity is recovered from the outer tube cavity of the red blood cell-poor PRP preparation device by the sampling device.

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