Bottled beverage including vitamin b12 and carnosine

Abstract

The present invention provides a novel food and drink containing at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. According to an embodiment of the present invention, there is provided a bottled beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the content of carnosine is 0.70-15 mg / mL and the content of the at least one cobalamin is 0.005-1 µm / mL.

Description

Packaged beverage containing vitamin B12 and carnosine

[0001] The present invention relates to vitamin B １２ The present invention relates to a packaged beverage containing at least one cobalamin selected from the group consisting of cobalamins, such as cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, and carnosine, and a method for producing the same. The present invention also relates to a method for stabilizing cobalamin and a method for improving the aftertaste of carnosine. The present invention also relates to an agent for inhibiting the degradation of cobalamin.

[0002] In recent years, with the increasing health consciousness, foods and beverages containing various nutritional components are being manufactured and sold. For example, foods and beverages containing various vitamins and minerals are included. There are various vitamins added to foods and beverages, and common examples include vitamin A, B vitamins, vitamin C, vitamin E, and vitamin K. Examples of B vitamins include vitamin B １ , Vitamin B ２ , Vitamin B ６ , Vitamin B １２ , niacin, pantothenic acid, folic acid and biotin are commonly used.

[0003] Each vitamin has a known nutritional function. According to the website of the Consumer Affairs Agency of Japan, vitamin A is listed as a nutrient that helps maintain healthy skin and mucous membranes, and vitamin B １ is described as a nutrient that helps produce energy from carbohydrates and maintain healthy skin and mucous membranes, and is a B vitamin １２ is described as a nutrient that aids in the formation of red blood cells.

[0004] On the other hand, some vitamins are easily decomposed, and much research is being done to stabilize these vitamins in food and drink. For example, vitamin B １２ can be destabilized by heat or other ingredients in foods and beverages, accelerating their decomposition.

[0005] Japanese Patent Application Laid-Open No. 2019-110827 (Patent Document 1) describes the use of vitamin B in an acidic composition. １２However, it has been discovered that the presence of ethanol increases the decomposition rate and / or decreases the stability of vitamin B. １２ In Japanese Patent Laid-Open No. 2022-162843 (Patent Document 2), an acidic composition containing vitamin B １２ is decomposed in an acidic aqueous solution, and a concentrate for a water-soluble vitamin drink has been proposed, which comprises (1) a water-soluble vitamin, (2) one or more selected from polyoxyethylene hydrogenated castor oil and polyoxyethylene castor oil, (3) one or more selected from the group consisting of polyglycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene glycerin fatty acid ester, sucrose fatty acid ester, and polyoxyethylene alkyl ether, and (4) a polyhydric alcohol. １２ It has been reported that glucose, amino acids, and vitamin B are greatly lost by heating. １ , ascorbyl palmitate and / or vitamin B essentially free of vitamin C １２ Formulations have been proposed.

[0006] Japanese Patent Application Laid-Open No. 2019-110827 Japanese Patent Application Laid-Open No. 2022-162843 Special Publication No. 2023-542821

[0007] Under the circumstances described above, there is a need for the development of new foods and beverages containing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin.

[0008] The present inventors have found that adding a predetermined amount of carnosine to a packaged beverage containing a predetermined amount of cobalamin can suppress the decomposition of cobalamin. Furthermore, the present inventors have found that cobalamin can improve the undesirable taste of carnosine. The present invention is based on these findings.

[0009] The present invention includes, for example, the following aspects. [1] A packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL, and the at least one cobalamin content is 0.005 to 1 μg / mL. [2] The packaged beverage according to [1], which contains chicken extract. [3] The packaged beverage according to [1] or [2], wherein the at least one cobalamin contains cyanocobalamin. [4] The packaged beverage according to any one of [1] to [3], which has a carnosine content of 0.75 to 7 mg / mL. [5] The packaged beverage according to any one of [1] to [4], which has a carnosine content of 0.80 to 4 mg / mL. [6] The packaged beverage according to any one of [1] to [5], wherein the content of the at least one cobalamin is 0.04 to 0.4 μg / mL. [7] The packaged beverage according to any one of [1] to [6], wherein the weight ratio of the content of carnosine to the content of the at least one cobalamin is 500:1 to 50,000:1. [8] The packaged beverage according to any one of [1] to [7], wherein after a retort test at 120°C for 8 minutes, 80% by weight or more of the content of the at least one cobalamin is maintained relative to the content of the at least one cobalamin before the retort test. [9] The packaged beverage according to any one of [1] to [8], which is a ready-to-drink (RTD) beverage, a soft drink, chicken soup, a jelly-like beverage, or a functional beverage.

[10] A method for producing the packaged beverage according to any one of [1] to [9], comprising adding at least one of the at least one cobalamin and carnosine to a beverage base.

[11] A method for stabilizing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, comprising adding carnosine to a solution containing the at least one type of cobalamin at a concentration of 0.01 to 15 mg / mL.

[12] A method for improving the aftertaste of carnosine, comprising adding at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin to a solution containing 0.01 to 15 mg / mL of carnosine to give a concentration of 0.005 to 1 μg / mL. [3] A packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the at least one cobalamin content is 0.005 to 1 μg / mL, and the propylene glycol content is less than 0.3 mg / mL.

[14] The packaged beverage according to any one of [1] to [9] and

[13] , wherein the ethanol content is less than 0.001 mg / mL.

[15] An inhibitor of the degradation of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, comprising carnosine.

[0010] According to one aspect of the present invention, a packaged beverage can be provided in which the stability of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin is improved. According to another aspect of the present invention, a packaged beverage can be provided in which the undesirable taste quality of carnosine is improved and which contains at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin.

[0011] Figure 1 is a graph showing the percent retention of cyanocobalamin in each experimental sample, relative to the amount of cyanocobalamin in the control sample (not retorted), set at 100. Figure 2 shows the average percent retention at various carnosine concentrations, based on data from experimental samples containing 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL of cyanocobalamin.

[0012] The present invention will be described in detail below. The following embodiments are illustrative of the present invention and are not intended to limit the present invention to these embodiments. The present invention can be embodied in various forms without departing from the spirit of the present invention. Any paragraph headings used herein are for organizational purposes only and should not be construed as limiting the subject matter described. All documents cited herein, as well as published publications, patent publications, and other patent documents, are incorporated herein by reference. This specification also incorporates the contents of the specification of Japanese Patent Application No. 2024-091512, filed on June 5, 2024, from which priority is claimed by this application.

[0013] 1. Beverage Comprising Cobalamin and Carnosine One aspect of the present invention provides a beverage comprising cobalamin and carnosine (hereinafter also referred to as the "beverage of the present invention"). According to a first aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL, and the at least one cobalamin content is 0.005 to 1 μg / mL.

[0014] According to a second aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the content of the at least one cobalamin is 0.005 to 1 μg / mL, and the propylene glycol content is less than 0.3 mg / mL.

[0015] Specific embodiments of the first and second aspects of the present invention will be described below, but unless otherwise specified, the following embodiments apply to both the first and second aspects.

[0016] The inventors have surprisingly found that the stability of cobalamin is improved by controlling the cobalamin content within a specific range and the carnosine content within a specific range. Without wishing to be bound by theory, it is speculated that in the basic structure of cobalamin, a cobalt ion is coordinated to a dimethylbenzimidazole moiety. While this dimethylbenzimidazole is sometimes replaced with histidine containing an imidazole moiety, carnosine, which also contains an imidazole moiety, chelates the cobalt ion to form a stable carnosine-cobalt complex. As demonstrated in the Examples below, increasing the concentration of carnosine tends to improve the stability of cyanocobalamin (a type of cobalamin), while increasing the concentration of carnosine tends to decrease the stability of cyanocobalamin. Although we do not wish to be bound by theory on this point either, it is speculated that when the concentration of carnosine becomes too high, the coordination of dimethylbenzimidazole is lost, resulting in an unstable structure of cobalamin.

[0017] Beverages according to preferred embodiments of the present invention are capable of suppressing the decomposition of the at least one cobalamin due to high-temperature heat sterilization treatment such as a retort test. After a retort test at 120°C for 8 minutes, a beverage according to one embodiment of the present invention maintains more than 70% by weight of the at least one cobalamin, relative to the content of the at least one cobalamin before the retort test. After a retort test at 120°C for 8 minutes, a beverage according to preferred embodiments of the present invention maintains 75% by weight or more, 80% by weight or more, 85% by weight or more, 90% by weight or more, 92% by weight or more, 94% by weight or more, 96% by weight or more, 98% by weight or more, or approximately 100% by weight of the at least one cobalamin, relative to the content of the at least one cobalamin before the retort test.

[0018] The inventors have also unexpectedly found that the undesirable taste of carnosine can be suppressed by adjusting the content of carnosine and at least one of the cobalamins within a specific range.The mechanism of human taste is complex, and it was completely unexpected that the taste of carnosine could be improved by the presence of a very small amount of at least one of the cobalamins.

[0019] <Cobalamin> Cobalamin is a B vitamin １２ Cobalamin is a general term for a group of vitamins classified as water-soluble. Cobalamin is a complex of cobalt. The most commonly used cobalamin is cyanocobalamin, whose molecular formula is C 63 H 88 CoN 14 O 14 P. Its CAS number is 68-19-9. Cobalamins other than cyanocobalamin include hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. Hydroxocobalamin is a cobalamin that has a hydroxyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C. 62 H 89 CoN 13 O 15P and its CAS number is 13422-51-0. Adenosylcobalamin is a cobalamin that has an adenosyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C 72 H 100 CoN 18 O 17 P and its CAS number is 13870-90-1. Methylcobalamin is a cobalamin with a methyl group instead of the cyano group of cyanocobalamin, and its molecular formula is C 63 H 91 CoN 13 O 14 P and its CAS number is 13422-55-4. Sulfitocobalamin is a cobalamin that has a sulfite group instead of the cyano group of cyanocobalamin, and its molecular formula is C 62 H 89 CoN 13 O 17 Its CAS number is 15671-27-9. A beverage according to one embodiment of the present invention comprises at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin. In one embodiment of the present invention, the at least one cobalamin preferably comprises cyanocobalamin and / or hydroxocobalamin, and more preferably comprises cyanocobalamin.

[0020] The origin of the at least one cobalamin used in the present invention is not particularly limited. Therefore, in the present invention, chemically synthesized cobalamin, cobalamin biosynthesized from a bacterial culture medium, or cobalamin isolated from a natural source containing cobalamin may be used. Alternatively, cobalamin may be added to the beverage by adding a food material containing cobalamin. Examples of food materials rich in cobalamin include seafood (especially clams and short-necked clams) and animal meat (e.g., chicken liver).

[0021] In the beverage according to one aspect of the present invention, the content of at least one of the above cobalamins is 0.005 to 1 μg / mL. The content of at least one of the above cobalamins is, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.005 to 0.8 μg / mL, 0.01 to 0.8 μg / mL, 0.02 to 0.8 μg / mL, ०.03 to 0.8 μg / mL, 0.04 to 0.8 μg / mL, 0.05 to 0.8 μg / mL, 0.06 to 0.8 μg / mL, 0.07 to 0.8 μg / mL, 0.08 to 0.8 μg / mL, 0.09 to 0.8 μg / mL, 0.1 to 0.8 μg / mL, 0.15 to 0.8 μg / mL, 0.2 to 0.8 μg / mL, 0.25 to 0.8 μg / mL, 0.3 to 0.8 μg / mL, 0.35 to 0.8 μg / mL, 0.4 to 0.8 μg / mL, 0.005 to 0.6 μg / mL, ०.01 to 0.6 μg / mL, 0.02 to 0.6 μg / mL, 0.03 to 0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL,०.07 to 0.6 μg / mL, 0.08 to 0.6 μg / mL, 0.09 to 0.6 μg / mL, 0.1 to 0.6 μg / mL, 0.15 to 0.6 μg / mL, 0.2 to 0.6 μg / mL, 0.25 to 0.6 μg / mL, 0.3 to 0.6 μg / mL, 0.35 to 0.6 μg / mL, 0.4 to 0.6 μg / mL, 0.005 to 0.5 μg / mL, 0.01 to 0.5 μg / mL, 0.02 to 0.५ μg / mL, 0.03 to 0.5 μg / mL, 0.04 to 0.5 μg / mL, 0.05 to 0.5 μg / mL, 0.06 to 0.5 μg / mL, 0.07 to 0.5 μg / mL, 0.08 to 0.5 μg / mL, 0.09 to 0.5 μg / mL, 0.1 to 0.5 μg / mL, 0.15 to०.5 μg / mL, 0.2 to 0.5 μg / mL, 0.25 to 0.5 μg / mL, 0.3 to 0.5 μg / mL, 0.35 to 0.5 μg / mL, 0.4 to 0.5 μg / mL, 0.005 to 0.4 μg / mL, It should be noted that there seems to be a "०" in the original text which might be a typo. I've translated it as is for now. If it's a specific symbol with a defined meaning, please let me know for a more accurate translation.0.01-0.4μg / mL, 0.02-0.4μg / mL, 0.03-0.4μg / mL, 0.04-0.4μg / mL, 0.05-0.4μg / mL, 0.06-0.4μg / m L, 0.07-0.4μg / mL, 0.08-0.4μg / mL, 0.09-0.4μg / mL, 0.1-0.4μg / mL, 0.15-0.4μg / mL, 0.2-0.4μg / mL , 0.25-0.4μg / mL, 0.3-0.4μg / mL, 0.35-0.4μg / mL, 0.005-0.2μg / mL, 0.01-0.2μg / mL, 0.02-0.2μg / mL mL, 0.03-0.2μg / mL, 0.04-0.2μg / mL, 0.05-0.2μg / mL, 0.06-0.2μg / mL, 0.07-0.2μg / mL, 0.08-0.2μg / mL, 0.09-0.2μg / mL, 0.1-0.2μg / mL, 0.15-0.2μg / mL, 0.005-0.1μg / mL, 0.01-0.1μg / mL, 0.02-0.1μg / mL, 0.03-0.1μg / mL, 0.04-0.1μg / mL, 0.05-0.1μg / mL, 0.06-0.1μg / mL, 0.07-0.1μg / mL, 0.08-0. The concentration may be 1 μg / mL, 0.09 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The content of the at least one cobalamin is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL. When the beverage contains only one type of cobalamin, this content refers to the content of the cobalamin contained therein. When the beverage contains two or more types of cobalamin, this content refers to the total content.

[0022] According to one aspect of the present invention, the content of cyanocobalamin in the food may be 0.005~1μg / mL, for example, 0.01~1μg / mL, 0.02~1μg / mL, 0.03~1μg / mL, 0.04~1μg / mL, 0.06~1μg / mL. mL、0.07~1μg / mL、0.08~1μg / mL、0.09~1μg / mL、0.1~1μg / mL、0.15~1μg / mL、0.2~1μg / mL ~0.8μg / mL、0.01~0.8μg / mL、0.02~0.8μg / mL、0.03~0.8μg / mL、0.04~0.8μg / mL、0.05~0.8μg / mL、0.07~0.8μg / mL 09~0.8μg / mL、0.1~0.8μg / mL、0.1~0.8μg / mL、0.2~0.8μg / mL、0.25~0.8μg / mL、0.3~0.8μg / mL、0.2~0... 1~0.6μg / mL、0.02~0.6μg / mL、0.03~0.6μg / mL、0.04~0.6μg / mL、0.05~0.6μg / mL、0.0~0.6μg / mL、0.09~0.6μg / mL、 0.1~0.6μg / mL、0.1~0.6μg / mL、0.2~0.6μg / mL、0.2~0.6μg / mL、0.3~0.6μg / mL、0.1~0.6μg / mL . 0.15~0.5μg / mL、0.2~0.5μg / mL、0.2~0.5μg / mL、0.3~0.5μg / mL、0.35~0.5μg / mL、0.2~0.5μg / mL0.03-0.4μg / mL, 0.04-0.4μg / mL, 0.05-0.4μg / mL, 0.06-0.4μg / mL, 0.07-0.4μg / mL, 0.08-0.4μ g / mL, 0.09-0.4 μg / mL, 0.1-0.4 μg / mL, 0.15-0.4 μg / mL, 0.2-0.4 μg / mL, 0.25-0.4 μg / mL, 0.3-0. 4μg / mL, 0.35-0.4μg / mL, 0.005-0.2μg / mL, 0.01-0.2μg / mL, 0.02-0.2μg / mL, 0.03-0.2μg / mL, 0 .04-0.2μg / mL, 0.05-0.2μg / mL, 0.06-0.2μg / mL, 0.07-0.2μg / mL, 0.08-0.2μg / mL, 0.09-0.2μg / mL, 0.1 to 0.2 μg / mL, 0.15 to 0.2 μg / mL, 0.005 to 0.1 μg / mL, 0.01 to 0.1 μg / mL, 0.02 to 0.1 μg / mL, 0.03 to 0.1 μg / mL, 0.04 to 0.1 μg / mL, 0.05 to 0.1 μg / mL, 0.06 to 0.1 μg / mL, 0.07 to 0.1 μg / mL, 0.08 to 0.1 μg / mL, The concentration may be 0.09 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The cyanocobalamin content is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL.

[0023] The content of the at least one cobalamin can be determined by liquid chromatography-mass spectrometry (LC-MS / MS). However, if the content can be calculated from the blend amount, it may also be determined from the blend amount. The measurement conditions for LC-MS / MS can be determined according to the standard measurement method of the analyzer. When measuring by LC-MS / MS, an Agilent 1290 Binary LC equipped with an AB Sciex 5600 and a TripleTOF system can be used. For example, a CORTECS T3 Column, 20 Å, 1.6 μm, 2.1 mm × 100 mm (manufactured by Waters Corporation) can be used as the LC column.

[0024] The packaged beverage according to one embodiment of the present invention comprises cyanocobalamin and at least one vitamin selected from the group consisting of hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin (hereinafter referred to as "other vitamin B"). １２ Other B vitamins are also included. １２ If vitamins are included, the content of cyanocobalamin and other vitamin B １２ The composition ratio of the total amount of vitamin B stearate, vitamin B stearate, and vitamin B stearate may be 99:1 to 90:10, 99:1 to 91:9, 99:1 to 92:8, 99:1 to 93:7, 99:1 to 94:6, 99:1 to 95:5, 99:1 to 96:4, 99:1 to 97:3, or 99:1 to 98:2 by mass. １２ Vitamin B １２ As used herein, "consisting essentially of" refers to the vitamin B stearate used in the present invention. １２ Vitamin B other than cyanocobalamin １２ This means that it may contain small amounts of vitamins such as vitamin B １２ When the composition essentially consists of cyanocobalamin, the content of cyanocobalamin is １２ This means that the content of cyanocobalamin is preferably 85 to 100% by weight, more preferably 90 to 100% by weight, and even more preferably 95 to 100% by weight, based on the total weight of the ingredients.１２ 100% by weight based on the total weight of the group means vitamin B １２ The group consists of cyanocobalamin and any other B vitamins １２ This means that the content of these compounds is an impurity amount (less than 1% by weight).

[0025] Vitamin B １２ Cobalamin is known to have health benefits such as hematopoietic and nerve repair effects, so including cobalamin can be expected to improve eye fatigue, stiff shoulders, and neuralgia.

[0026] <Carnosine> In one embodiment of the present invention, the beverage contains carnosine. Carnosine is a type of imidazole peptide, and imidazole dipeptide is a general term for dipeptides whose constituent is histidine with an imidazole ring (Yanagiuchi Nobuya, Journal of the Japanese Society of Food Chemical Technology, 2014, Vol. 61, No. 1, p. 45). Here, imidazole is a type of five-membered aromatic heterocyclic compound, a nitrogen-containing aromatic heterocyclic compound with nitrogen atoms at the first and third positions.

[0027] In one embodiment of the present invention, the carnosine used in the beverage may be obtained by extraction with an aqueous solvent from a carnosine-containing material (e.g., fish meat) or by chemical synthesis, and its origin and raw materials are not particularly limited. Alternatively, carnosine may be added to the beverage by adding a carnosine-containing food ingredient. For example, carnosine is abundant in fish meat such as tuna, bonito, and salmon, as well as beef, pork, and chicken. Mammals such as pigs and cows contain a large amount of carnosine, while aquatic mammals contain carnosine and balenine in a ratio of about 1:2. Birds contain carnosine and anserine in a ratio of 1:3. Large fish contain almost exclusively anserine (Yanagiuchi, Nobuya, Journal of the Japanese Society of Food and Chemical Technology, 2014, Vol. 61, No. 1, p. 45). In a preferred embodiment of the present invention, chicken extract, described below, can be used as a carnosine-containing food ingredient.

[0028] The content of carnosine contained in the beverage in one embodiment of the first aspect of the present invention is 0.70 to 15 mg / mL, for example, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, 0.70 to 12 mg / mL, 0.75 to 12 mg / mL, 0.75 to 15 mg / mL, 0.75 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, 0.70 to 12 mg / mL, 2mg / mL, 0.80-12mg / mL, 0.90-12mg / mL, 1-12mg / mL, 1.5-12mg / mL, 2-12 mg / mL, 2.5-12 mg / mL, 3-12 mg / mL, 3.5-12 mg / mL, 4-12 mg / mL, 4.5-12 mg / mL mL, 5-12 mg / mL, 5.5-12 mg / mL, 6-12 mg / mL, 6.5-12 mg / mL, 7-12 mg / mL, 7. 5-12mg / mL, 8-12mg / mL, 0.70-10mg / mL, 0.75-10mg / mL, 0.80-10mg / mL, 0.90-10mg / mL, 1-10mg / mL, 1.5-10mg / mL, 2-10mg / mL, 2.5-10mg / mL, 3- 10mg / mL, 3.5-10mg / mL, 4-10mg / mL, 4.5-10mg / mL, 5-10mg / mL, 5.5-10m g / mL, 6-10 mg / mL, 6.5-10 mg / mL, 7-10 mg / mL, 7.5-10 mg / mL, 8-10 mg / mL, 0.70-8mg / mL, 0.75-8mg / mL, 0.80-8mg / mL, 0.90-8mg / mL, 1-8mg / mL, 1. 5-8mg / mL, 2-8mg / mL, 2.5-8mg / mL, 3-8mg / mL, 3.5-8mg / mL, 4-8mg / mL, 4 5-8 mg / mL, 5-8 mg / mL, 5.5-8 mg / mL, 6-8 mg / mL, 6.5-8 mg / mL, 7-8 mg / mL, 7.5-8mg / mL, 0.70-7mg / mL, 0.75-7mg / mL, 0.80-7mg / mL, 0.90-7mg / mL, 1-7 mg / mL, 1.5-7 mg / mL, 2-7 mg / mL, 2.5-7 mg / mL, 3-7 mg / mL, 3.5-7 mg / mL,4-7 mg / mL, 4.5-7 mg / mL, 5-7 mg / mL, 5.5-7 mg / mL, 6-7 mg / mL, 6.5-7 mg / mL, 0.70-6mg / mL, 0.75-6mg / mL, 0.80-6mg / mL, 0.90-6mg / mL, 1-6mg / mL, 1.5 ~6mg / mL, 2~6mg / mL, 2.5~6mg / mL, 3~6mg / mL, 3.5~6mg / mL, 4~6mg / mL, 4.5 ~6mg / mL, 5~6mg / mL, 5.5~6mg / mL, 0.70~4mg / mL, 0.75~4mg / mL, 0.80~4mg The carnosine content may be 0.75-7 mg / mL, more preferably 0.80-4 mg / mL, 1-4 mg / mL, 1.5-4 mg / mL, 2-4 mg / mL, 2.5-4 mg / mL, 3-4 mg / mL, 3.5-4 mg / mL, 0.70-3 mg / mL, 0.75-3 mg / mL, 0.80-3 mg / mL, 0.90-3 mg / mL, 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL.

[0029] According to one embodiment of the second aspect of the present invention, the carnosine content is 0.50 to 15 mg / mL, for example, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7 to 15 mg / mL, 7.5 to 15 mg / mL, 8 to 15 mg / mL, 9 to 15 mg / mL, 10 to 15 mg / mL, 11 to 15 mg / mL, 12 to 15 mg / mL, 13 to 15 mg / mL, 14 to 15 mg / mL, 15 to 15 mg / mL, 16 to 15 mg / mL, 17 to 15 mg / mL, 18 to 15 mg / mL, 19 to 20 mg / mL, 21 to 22 mg / mL, 22 to 23 mg / mL, 23 to 24 mg / mL, 24 to 25 mg / mL, 25 to 26 mg / mL, 26 to 27 mg / mL, 27 to 28 mg / mL, 28 to 29 mg / mL, 29 to 30 mg / mL, 30 to 31 mg / mL, 31 to 32 mg / mL, 32 to mg / mL, 0.50-12 mg / mL, 0.60-12 mg / mL, 0.70-12 mg / mL, 0.75-12 mg / mL, 0 .80-12mg / mL, 0.90-12mg / mL, 1-12mg / mL, 1.5-12mg / mL, 2-12mg / mL, 2.5 ~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12mg / mL, 5.5-12mg / mL, 6-12mg / mL, 6.5-12mg / mL, 7-12mg / mL, 7.5-12mg / mL, 8-12mg / mL, 0.50-10mg / mL, 0.60-10mg / mL, 0.70-10mg / mL, 0.75-10mg / mL, 0.80-10mg / mL, 0.90-10mg / mL, 1-10mg / mL, 1.5-10mg / mL, 2-10mg / mL , 2.5-10mg / mL, 3-10mg / mL, 3.5-10mg / mL, 4-10mg / mL, 4.5-10mg / mL, 5- 10mg / mL, 5.5-10mg / mL, 6-10mg / mL, 6.5-10mg / mL, 7-10mg / mL, 7.5-10mg / mL, 8-10mg / mL, 0.50-8mg / mL, 0.60-8mg / mL, 0.70-8mg / mL, 0.75-8mg / mL, 0.80-8mg / mL, 0.90-8mg / mL, 1-8mg / mL, 1.5-8mg / mL, 2-8mg / mL, 2.5-8mg / mL, 3-8mg / mL, 3.5-8mg / mL, 4-8mg / mL, 4.5-8mg / mL, 5-8mg / mL, 5.5-8mg / mL, 6-8mg / mL, 6.5-8mg / mL, 7-8mg / mL, 7.5-8mg / mL, 0.50-7mg / mL,0.60-7mg / mL, 0.70-7mg / mL, 0.75-7mg / mL, 0.80-7mg / mL, 0.90-7mg / mL, 1-7mg / mL, 1.5-7mg / mL, 2-7mg / m L, 2.5-7mg / mL, 3-7mg / mL, 3.5-7mg / mL, 4-7mg / mL, 4.5-7mg / mL, 5-7mg / mL, 5.5-7mg / mL, 6-7mg / mL, 6.5-7 mg / mL, 0.50-6 mg / mL, 0.60-6 mg / mL, 0.70-6 mg / mL, 0.75-6 mg / mL, 0.80-6 mg / mL, 0.90-6 mg / mL, 1-6 mg / mL, 1.5-6mg / mL, 2-6mg / mL, 2.5-6mg / mL, 3-6mg / mL, 3.5-6mg / mL, 4-6mg / mL, 4.5-6mg / mL, 5-6mg / mL, 5.5-6mg / mL, 0.50-4mg / mL, 0.60-4mg / mL, 0.70-4mg / mL, 0.75-4mg / mL, 0.80-4mg / mL, 0.90-4mg / mL, 1-4mg / mL, 1.5-4mg / mL, 2-4mg / mL, 2.5-4mg / mL, 3-4mg / mL, 3.5-4mg / mL, 0.50-3mg / mL, 0.60-3mg / mL, 0.70-3mg / mL, 0. The carnosine content may be 75-3 mg / mL, 0.80-3 mg / mL, 0.90-3 mg / mL, 1-3 mg / mL, 1.5-3 mg / mL, 2-3 mg / mL, 2.5-3 mg / mL, 0.50-2 mg / mL, 0.60-2 mg / mL, 0.70-2 mg / mL, 0.75-2 mg / mL, 0.80-2 mg / mL, 0.90-2 mg / mL, 1-2 mg / mL, or 1.5-2 mg / mL. The carnosine content is preferably 0.75-7 mg / mL, more preferably 0.80-4 mg / mL.

[0030] The carnosine content can be determined by high-performance liquid chromatography (HPLC), or if the content can be calculated from the blended amount, it can also be determined from the blended amount. The measurement conditions for HPLC can be the standard measurement method of the analyzer. When measuring by HPLC, an ion exchange column (e.g., 300SCX (dimensions: 4.6 × 250 mm, particle size: 5 μm)) can be used.

[0031] The ratio of carnosine to the at least one cobalamin is not particularly limited, but in a preferred embodiment of the present invention, the weight ratio of the carnosine content to the at least one cobalamin content is 500:1 to 50,000:1. In another embodiment of the present invention, the weight ratio of the carnosine content to the at least one cobalamin content may be 1,000:1 to 50,000:1, 2,000:1 to 50,000:1, 4,000:1 to 50,000:1, 5,000:1 to 50,000:1, 8,000:1 to 50,000:1, 8,000:1 to 40,000:1, 8,000:1 to 35,000:1, or 8,000:1 to 30,000:1. Furthermore, from the viewpoint of improving the undesirable taste of carnosine, the weight ratio of the content of carnosine to the content of at least one of the above-mentioned cobalamins is preferably 4000:1 to 15000:1, more preferably 6000:1 to 12000:1, and even more preferably 6500:1 to 10000:1.

[0032] <Chicken Extract> A beverage according to one embodiment of the present invention contains chicken extract. Chicken extract is a component obtained by hot water extraction or the like using chicken meat, bones, skin, and other parts as raw materials. Chicken extract generally contains carnosine.

[0033] When the chicken extract used in the beverage of one embodiment of the present invention contains a carnosine salt, the content of carnosine in the chicken extract means the total amount of carnosine and its salt.

[0034] Examples of salts of carnosine include, but are not limited to, inorganic salts such as hydroxides, carbonates, and bicarbonates of sodium, lithium, calcium, magnesium, and aluminum and ammonia; and organic salts such as mono-, di-, or tri-alkylamine salts, such as methylamine, dimethylamine, and trimethylamine salts, mono-, di-, or tri-hydroxyalkylamine salts, guanidine salts, and N-methylglucosamine salts.

[0035] When a chicken extract is used in a beverage according to one embodiment of the present invention, an extract obtained by heating raw materials such as chicken meat in a liquid may be used, or a commercially available chicken extract may be used. Raw materials for the chicken extract may include chicken bones, cartilage, feet (also known as maple leaves), etc.

[0036] Commercially available chicken extracts include "Brand's Essence of Chicken (BEC) (manufactured by Suntory Beverage & Food Asia Co., Ltd.)," "Scotch® Essence of Chicken (manufactured by Scotch Industrial Co., Ltd., Thailand)," "Quaker Essence of Chicken (manufactured by Standard Foods Corporation, Taiwan)," "SWANSON® Chicken Stock and Broth (manufactured by Campbell Soup Company)," "Drip Chicken Essence (manufactured by Eu Yan Sang International Co., Ltd., Singapore)," "Boned Chicken Tonic (manufactured by Eu Yan Sang International Co., Ltd., Singapore)," and "Boiled Essence of Chicken (manufactured by Lao Xie Zhen Co., Ltd., Taiwan)." While any of these commercially available products can be used, it is preferable to use Brand's Essence of Chicken (BEC).

[0037] When the chicken extract used in the beverage of one embodiment of the present invention is produced by hot water extraction of chicken meat, chicken bones, cartilage, feet, etc. (hereinafter referred to as "chicken meat, etc."), it can be produced by a commonly used method. For example, chicken extract can be produced by treating chicken meat, etc. in a solvent at high temperature under normal or increased pressure. When producing chicken extract, fat and cholesterol may be removed after crude extraction with a solvent, and then excess solvent may be removed to increase the concentration.

[0038] As used herein, chicken extract includes the liquid extract, diluted solution, concentrate, or dry powder obtained by the above-mentioned method, as well as purified products thereof. The purified product can be obtained, for example, by subjecting the chicken extract to diatomaceous earth filtration, ultrafiltration, membrane treatment, liquid separation, or fractionation using resins to achieve high purification.

[0039] In one embodiment of the present invention, the amount of chicken extract (in terms of solid content) in the beverage is not particularly limited, but is preferably 5% to 20% by weight. In a preferred embodiment of the present invention, the amount of chicken extract (in terms of solid content) may be 6% to 14% by weight, 8% to 12% by weight, or 9% to 11% by weight.

[0040] <Beverage Form> The beverage of the present invention is a packaged beverage. The type of container for the beverage is not limited, and the beverage may be packaged in a container such as a can, bottle, PET bottle, pouch, paper carton, or plastic container. According to a preferred embodiment of the present invention, the beverage is packaged in a small-portion pouch for convenient, regular consumption. When heat sterilization is performed after packaging, the type is not particularly limited, and can be carried out using conventional techniques such as inversion sterilization, UHT sterilization, and retort sterilization. The temperature of the heat sterilization step is not particularly limited, but is, for example, 65 to 130°C, preferably 80 to 120°C, for 1 to 40 minutes. However, as long as a sterilization value equivalent to that under the above conditions is obtained, sterilization at an appropriate temperature for a few seconds, for example, 5 to 30 seconds, is acceptable.

[0041] In one embodiment of the present invention, beverages include ready-to-drink (RTD) beverages, soft drinks, soups containing meat extracts (extracts obtained from chicken, beef, pork, etc.), such as chicken soup, jelly-like beverages, powdered beverages, and functional beverages. As used herein, powdered beverages refer to powdered beverages that are diluted with water or hot water to a predetermined concentration before consumption. Beverages containing chicken extract also include compositions that are heated and consumed in a soup-like state, such as pouched soups (e.g., chicken essence or DEOC (Drip Essence of Chicken)). In a preferred embodiment of the present invention, the packaged beverage is a ready-to-drink (RTD) beverage.

[0042] <Propylene Glycol> A beverage according to one embodiment of the present invention contains propylene glycol, while a beverage according to another embodiment of the present invention does not contain propylene glycol. When propylene glycol is contained, the content may be, for example, less than 1 mg / mL, less than 0.8 mg / mL, less than 0.6 mg / mL, less than 0.4 mg / mL, less than 0.3 mg / mL, or less than 0.2 mg / mL. A beverage according to a second embodiment of the present invention contains carnosine and at least one of the above-mentioned cobalamins, and has a propylene glycol content of less than 0.3 mg / mL. The propylene glycol content is not particularly limited as long as it is less than 0.3 mg / mL, and may be 0 mg / mL (or below the lower limit of measurement), but may also be, for example, 0.15 mg / mL or less, 0.12 mg / mL or less, 0.10 mg / mL or less, or 0.08 mg / mL or less. Preferably, the propylene glycol content is 0 to 0.08 mg / mL. The amount of propylene glycol can be adjusted by selecting the ingredients to be added. For example, the amount of propylene glycol can be reduced by not adding fragrances, and the propylene glycol content can be measured by HS-SPME-GC-MS (headspace solid-phase microextraction gas chromatography-mass spectrometry).

[0043] <Other Ingredients and Properties> As described in Patent Document 1, ethanol promotes the decomposition of cobalamin, so its content is preferably limited. The ethanol content is preferably less than 0.001 mg / mL, and may be less than 0.0008 mg / mL or less than 0.0005 mg / mL. The amount of ethanol can be adjusted by selecting the ingredients to be added. For example, the amount of ethanol can be reduced by not adding flavorings. The ethanol content can be measured by HS-SPME-GC-MS.

[0044] A beverage according to one embodiment of the present invention is substantially free of one or both of copper and vitamin C. As used herein, "substantially free of copper" means that the iron content is less than 1 mM, and "substantially free of vitamin C" means that the vitamin C content is less than 10 mM. According to one embodiment of the present invention, the copper content in the beverage is less than 0.1 mM, and the vitamin C content is less than 1 mM.

[0045] The beverage according to one embodiment of the present invention contains caramel. The beverage according to another embodiment of the present invention does not contain melon juice. The beverage according to yet another embodiment of the present invention does not contain niacinamide or has a niacinamide content of less than 26 mg / 100 ml, less than 20 mg / 100 ml, or less than 15 mg / 100 ml. The beverage according to yet another embodiment of the present invention contains vitamin B ２ Does not contain riboflavin or vitamin B ２ The beverage according to still another aspect of the present invention is free of pyridoxine hydrochloride or has a pyridoxine hydrochloride content of less than 5.0 mg / 100 ml, less than 4.0 mg / 100 ml, or less than 2.0 mg / 100 ml.

[0046] The beverage according to one embodiment of the present invention is a low-calorie beverage. Here, a low-calorie beverage refers to, for example, a beverage with a calorie content of 20 kcal / bottle or less, preferably 18 kcal / bottle or less, and more preferably 16 kcal / bottle or less. The calorie content of a beverage can be calculated by calculating the content of each ingredient contained in the beverage and multiplying the calculated content by an energy conversion factor, or by measuring the physical heat of combustion using a calorie meter (e.g., a bomb calorie meter) and correcting the calculated value for digestion and absorption rate, excreted heat, etc.

[0047] The beverage according to one embodiment of the present invention contains water. The water is not particularly limited, and any type of water can be used as long as it does not adversely affect the flavor, including tap water, ion-exchanged water, softened water, distilled water, carbonated water, reverse osmosis water (RO water), activated carbon-treated water, purified water, and demineralized water.

[0048] The pH of the beverage according to one embodiment of the present invention may be preferably 4 to 7, 4.5 to 7, 5 to 7, 5.5 to 7, 6 to 7, 4 to 6.8, 4.5 to 6.8, 5 to 6.8, 5.5 to 6.8, 6 to 6.8, 4 to 6.6, 4.5 to 6.6, 5 to 6.6, 5.5 to 6.6, or 6 to 6.6. By adjusting the pH to within this range, a pleasant taste with less sourness can be achieved.

[0049] The Brix (soluble solids content) of a beverage according to one embodiment of the present invention is preferably 5 to 20, more preferably 6 to 14, and even more preferably 8 to 12. The Brix (soluble solids content) of a beverage can be measured using a sugar content refractometer.

[0050] The beverage according to one embodiment of the present invention may contain a sweetener. The sweetener may be a low-intensity sweetener, a high-intensity sweetener, or a combination thereof. Examples of low-intensity sweeteners include glucose, sucrose (e.g., granulated sugar), fructose, maltose, oligosaccharides, high-fructose corn syrup, lactose, psicose, allose, tagatose, xylose, and ribose. Examples of high-intensity sweeteners include aspartame, neotame, advantame, sucralose, acesulfame potassium (acesulfame K), saccharin, saccharin sodium, sodium cyclamate, dulcin, disodium glycyrrhizinate, trisodium glycyrrhizinate, neohesperidin dihydrochalcone, thaumatin, monellin, curculin, mabinlin, brazzein, pentadine, hernandulcin, 4β-hydroxyhernandulcin, miraculin, glycyrrhizin, rubusoside, phyllodulcin, mogroside IV, mogroside V, steviol glycosides (rebaudioside A, rebaudioside D, rebaudioside M), thaumatin, monellin, brazzein, monatin, monk fruit extract, and stevia extract.

[0051] The beverage according to one embodiment of the present invention may contain, in addition to the above-mentioned components, components that can be added to ordinary beverages, as long as the effects of the present invention are not impaired. Examples of such components include acidulants, flavorings, vitamins, colorings, antioxidants, emulsifiers, preservatives, seasonings, extracts, pH adjusters, and quality stabilizers.

[0052] [Examples of the beverage of the present invention] The following are examples of the beverage of the present invention, but the present invention is not limited to the following examples. According to one aspect of the present invention, carnosine and vitamin B １２According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and vitamin B 2, wherein the carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and the content of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin is 0.005 to 1 μg / mL. １２ The present invention provides a packaged beverage comprising the above, wherein the carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and the cyanocobalamin content is 0.005 to 1 μg / mL.

[0053] According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and the at least one cobalamin content is 0.04 to 0.4 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and the cyanocobalamin content is 0.04 to 0.4 μg / mL.

[0054] According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, the packaged beverage containing chicken extract, having a carnosine content of 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and having a cyanocobalamin content of 0.005 to 1 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, the packaged beverage containing chicken extract, having a carnosine content of 0.70 to 15 mg / mL, preferably 0.75 to 7 mg / mL, and more preferably 0.80 to 4 mg / mL, and having a cyanocobalamin content of 0.005 to 1 μg / mL.

[0055] According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, the packaged beverage containing chicken extract, having a carnosine content of 0.75 to 7 mg / mL, and having a content of the at least one cobalamin of 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, the packaged beverage containing chicken extract, having a carnosine content of 0.75 to 7 mg / mL and a cyanocobalamin content of 0.005 to 1 μg / mL, preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL, or 0.005 to 0.09 μg / mL.

[0056] According to one aspect of the present invention, there is provided a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the at least one cobalamin content is 0.005 to 1 μg / mL, and the propylene glycol content is less than 0.3 mg / mL, preferably 0.15 mg / mL or less, or 0.08 mg / mL or less. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the cyanocobalamin content is 0.005 to 1 μg / mL, and the propylene glycol content is less than 0.3 mg / mL, preferably 0.15 mg / mL or less or 0.08 mg / mL or less.

[0057] According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the at least one cobalamin content is 0.005 to 1 μg / mL, and the propylene glycol content is 0.15 mg / mL or less, and the beverage satisfies the following requirements: (i) it does not contain melon juice; (ii) it does not contain niacinamide or the niacinamide content is less than 26 mg / 100 ml; and (iii) it is a vitamin B ２ Does not contain or contains vitamin B ２and (iv) being free of pyridoxine hydrochloride or having a pyridoxine hydrochloride content of less than 5.0 mg / 100 ml. According to one aspect of the present invention, there is provided a packaged beverage containing carnosine and cyanocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the cyanocobalamin content is 0.005 to 1 μg / mL, and the propylene glycol content is 0.15 mg / mL or less, and the beverage satisfies the following requirements: (i) not containing melon juice; (ii) not containing niacinamide or having a niacinamide content of less than 26 mg / 100 ml; (iii) vitamin B ２ Does not contain or contains vitamin B ２ (iv) the content of pyridoxine hydrochloride is less than 3.0 mg / 100 ml; and (iv) the content of pyridoxine hydrochloride is either free of pyridoxine hydrochloride or less than 5.0 mg / 100 ml.

[0058] 2. Method for Producing Beverage One aspect of the present invention provides a method for producing a packaged beverage comprising carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL and the at least one cobalamin content is 0.005 to 1 μg / mL (hereinafter also referred to as the "method for producing the beverage of the present invention").

[0059] A method for producing a beverage according to one embodiment of the present invention includes adding at least one of the above-described cobalamin and carnosine to a beverage base. A method for producing a beverage according to a preferred embodiment of the present invention includes adding water to the carnosine of the present invention and the above-described at least one cobalamin to obtain a packaged beverage having a carnosine content of 0.70 to 15 mg / mL and a cobalamin content of 0.005 to 1 μg / mL.

[0060] In one embodiment of the present invention, a beverage manufacturing method can add a predetermined amount of carnosine to a beverage by adding ingredients rich in carnosine to the beverage. Regarding ingredients rich in carnosine, those described in the section "1. Beverages containing cobalamin and carnosine" can be used as appropriate.

[0061] In this specification, the term "beverage base" refers to a liquid that serves as a precursor to a beverage, and is prepared by adding predetermined ingredients to the beverage base and optionally subjecting it to predetermined processing, heat sterilization, etc. Examples of beverage bases include, but are not limited to, water, carbonated water, fruit juice, soup containing meat extract (extract obtained from chicken, beef, pork, etc.), such as chicken soup, sports drinks, or combinations thereof.

[0062] The production method according to one embodiment of the present invention may include a packaging step and a subsequent sterilization step. Packaging can be carried out by any known method. When heat sterilization is carried out after packaging, the type of heat sterilization is not particularly limited, and can be carried out using conventional methods such as UHT sterilization and retort sterilization. The temperature of the heat sterilization step is not particularly limited, but is, for example, 50 to 130°C, preferably 50 to 120°C, for 10 to 40 minutes. However, as long as a sterilization value equivalent to that under the above conditions is obtained, sterilization at an appropriate temperature for a few seconds, for example, 5 to 30 seconds, is not a problem.

[0063] In the method for producing a beverage of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" described in the section "1. Beverages containing cobalamin and carnosine" can be used. The form and characteristics of the beverage can also be determined based on the descriptions in the section "1. Beverages containing cobalamin and carnosine."

[0064] 3. Method for Stabilizing Cobalamin One aspect of the present invention provides the following method for stabilizing cobalamin (hereinafter also referred to as the "stabilization method of the present invention"). According to one aspect of the present invention, there is provided a method for stabilizing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein a solution containing the at least one type of cobalamin contains carnosine at a concentration of 0.01 to 15 mg / mL.

[0065] The present inventors have surprisingly found that the stability of cobalamin can be improved by adding carnosine to a solution containing cobalamin at a predetermined concentration. For example, the stabilization method according to one embodiment of the present invention can suppress the decomposition of cobalamin during heat treatment such as retort sterilization.

[0066] In one embodiment of the stabilization method of the present invention, the concentration of carnosine in the solution containing at least one cobalamin is 0.01 to 15 mg / mL. In another embodiment, the concentration of carnosine is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, 5 to 15 mg / mL, 6 to 15 mg / mL, 7 to 15 mg / mL, 8 to 15 mg / mL, 9 to 15 mg / mL, 10 to 15 mg / mL, 11 to 15 mg / mL, 12 to 15 mg / mL, 13 to 15 mg / mL, 14 to 15 mg / mL, 15 to 15 mg / mL, 16 to 15 mg / mL, 17 to 15 mg / mL, 18 to 15 mg / mL, 19 to 20 mg / mL, 21 to 22 mg / mL, 22 to 23 mg / mL, 23 to 24 mg / mL, 24 to 25 mg / mL, 25 to 26 mg / mL, 26 to 27 mg / mL, 27 to 28 ~15mg / mL, 4.5~15mg / mL, 5~15mg / mL, 5.5~15mg / mL, 6~15mg / mL, 6.5~15mg / mL, 7~15mg / mL, 7.5~15mg / mL, 8~15mg / mL, 0.01~12mg / mL, 0.05~ 12mg / mL, 0.10-12mg / mL, 0.20-12mg / mL, 0.30-12mg / mL, 0.40-12mg / m L, 0.50-12mg / mL, 0.60-12mg / mL, 0.70-12mg / mL, 0.75-12mg / mL, 0.80 ~12mg / mL, 0.90~12mg / mL, 1~12mg / mL, 1.5~12mg / mL, 2~12mg / mL, 2.5~12mg / mL, 3~12mg / mL, 3.5~12mg / mL, 4~12mg / mL, 4.5~12mg / mL, 5~12m g / mL, 5.5 to 12 mg / mL, 6 to 12 mg / mL, 6.5 to 12 mg / mL, 7 to 12 mg / mL, 7.5 to 12 mg / mL mL, 8-12 mg / mL, 0.01-10 mg / mL, 0.05-10 mg / mL, 0.10-10 mg / mL, 0.20-1 0mg / mL, 0.30-10mg / mL, 0.40-10mg / mL, 0.50-10mg / mL, 0.60-10mg / mL, 0.70-10mg / mL, 0.75-10mg / mL, 0.80-10mg / mL, 0.90-10mg / mL, 1-10 mg / mL, 1.5-10 mg / mL, 2-10 mg / mL, 2.5-10 mg / mL, 3-10 mg / mL, 3.5-10 mg / mL, 4-10mg / mL, 4.5-10mg / mL, 5-10mg / mL, 5.5-10mg / mL, 6-10mg / mL,6.5~10mg / mL、7~10mg / mL、7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75-4mg / mL, 0.80-4mg / mL, 0.90-4mg / mL, 1-4mg / mL, 1.5-4mg / mL, 2-4mg / mL, 2.5-4mg / mL, 3-4mg / mL, 3.5-4mg / mL, 0.01-3mg / mL, 0.05- 3mg / mL, 0.10~3mg / mL, 0.20~3mg / mL, 0.30~3mg / mL, 0.40~3mg / mL, 0.50~3mg / mL, 0.60~3mg / mL, 0.70~3mg / mL, 0.75~3mg / mL, 0.80~3mg / mL The concentration of carnosine may be 0.75-7 mg / mL or 0.80-4 mg / mL. A stabilization method according to one embodiment of the present invention includes adding carnosine to the above-described concentrations.

[0067] When adding carnosine to a solution containing at least one type of cobalamin as described above, it may be added by extracting carnosine from a material containing carnosine (e.g., fish or chicken) with an aqueous solvent, or by chemical synthesis. Alternatively, carnosine may be added by adding food ingredients containing carnosine to the beverage.

[0068] In the stabilization method according to one aspect of the present invention, the concentration of the at least one cobalamin in the solution containing the at least one cobalamin is not particularly limited, and may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, μg / mL, 0.3-1 μg / mL, 0.35-1 μg / mL, 0.4-1 μg / mL, 0.005-0.8 μg / mL, 0.01-0. 8μg / mL, 0.02-0.8μg / mL, 0.03-0.8μg / mL, 0.04-0.8μg / mL, 0.05-0.8μg / mL , 0.06-0.8μg / mL, 0.07-0.8μg / mL, 0.08-0.8μg / mL, 0.09-0.8μg / mL, 0.1-0 .8μg / mL, 0.15-0.8μg / mL, 0.2-0.8μg / mL, 0.25-0.8μg / mL, 0.3-0.8μg / mL, 0.35-0.8μg / mL, 0.4-0.8μg / mL, 0.005-0.6μg / mL, 0.01-0.6μg / mL, 0.02-0 .6μg / mL, 0.03-0.6μg / mL, 0.04-0.6μg / mL, 0.05-0.6μg / mL, 0.06-0.6μg / m L, 0.07-0.6μg / mL, 0.08-0.6μg / mL, 0.09-0.6μg / mL, 0.1-0.6μg / mL, 0.15- 0.6μg / mL, 0.2-0.6μg / mL, 0.25-0.6μg / mL, 0.3-0.6μg / mL, 0.35-0.6μg / mL , 0.4-0.6μg / mL, 0.005-0.5μg / mL, 0.01-0.5μg / mL, 0.02-0.5μg / mL, 0.03- 0.5μg / mL, 0.04-0.5μg / mL, 0.05-0.5μg / mL, 0.06-0.5μg / mL, 0.07-0.5μg / mL mL, 0.08-0.5μg / mL, 0.09-0.5μg / mL, 0.1-0.5μg / mL, 0.15-0.5μg / mL, 0.2- 0.5μg / mL, 0.25-0.5μg / mL, 0.3-0.5μg / mL, 0.35-0.5μg / mL, 0.4-0.5μg / mL,0.005-0.4μg / mL, 0.01-0.4μg / mL, 0.02-0.4μg / mL, 0.03-0.4μg / mL, 0.04-0.4μg / mL, 0.05-0.4μg / mL , 0.06-0.4μg / mL, 0.07-0.4μg / mL, 0.08-0.4μg / mL, 0.09-0.4μg / mL, 0.1-0.4μg / mL, 0.15-0.4μg / mL, 0 .. 2-0.4μg / mL, 0.25-0.4μg / mL, 0.3-0.4μg / mL, 0.35-0.4μg / mL, 0.005-0.2μg / mL, 0.01-0.2μg / mL, 0.0 2-0.2μg / mL, 0.03-0.2μg / mL, 0.04-0.2μg / mL, 0.05-0.2μg / mL, 0.06-0.2μg / mL, 0.07-0.2μg / mL, 0.0 8-0.2μg / mL, 0.09-0.2μg / mL, 0.1-0.2μg / mL, 0.15-0.2μg / mL, 0.005-0.1μg / mL, 0.01-0.1μg / mL, 0.0 2-0.1μg / mL, 0.03-0.1μg / mL, 0.04-0.1μg / mL, 0.05-0.1μg / mL, 0.06-0.1μg / mL, 0.07-0.1μg / mL, 0.08 The concentration of the at least one cobalamin may be 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL. When the beverage contains only one type of cobalamin, this concentration refers to the concentration of the cobalamin contained therein. When the beverage contains two or more types of cobalamin, this concentration refers to the total concentration of the cobalamins contained therein.

[0069] In the stabilization method according to one aspect of the present invention, the concentration of cyanocobalamin in the solution containing cyanocobalamin is not particularly limited, and may be, for example, 0.005 to 1 μg / mL, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, or 0.3 to 1 μg / mL , 0.35-1μg / mL, 0.4-1μg / mL, 0.005-0.8μg / mL, 0.01-0.8μg / mL, 0.02-0.8μ g / mL, 0.03-0.8μg / mL, 0.04-0.8μg / mL, 0.05-0.8μg / mL, 0.06-0.8μg / mL, 0 07-0.8μg / mL, 0.08-0.8μg / mL, 0.09-0.8μg / mL, 0.1-0.8μg / mL, 0.15-0.8 μg / mL, 0.2-0.8 μg / mL, 0.25-0.8 μg / mL, 0.3-0.8 μg / mL, 0.35-0.8 μg / mL, 0. 4-0.8μg / mL, 0.005-0.6μg / mL, 0.01-0.6μg / mL, 0.02-0.6μg / mL, 0.03-0.6 μg / mL, 0.04 to 0.6 μg / mL, 0.05 to 0.6 μg / mL, 0.06 to 0.6 μg / mL, 0.07 to 0.6 μg / mL, 0.08-0.6μg / mL, 0.09-0.6μg / mL, 0.1-0.6μg / mL, 0.15-0.6μg / mL, 0.2-0.6 μg / mL, 0.25-0.6 μg / mL, 0.3-0.6 μg / mL, 0.35-0.6 μg / mL, 0.4-0.6 μg / mL, 0. 005-0.5μg / mL, 0.01-0.5μg / mL, 0.02-0.5μg / mL, 0.03-0.5μg / mL, 0.04-0. 5μg / mL, 0.05-0.5μg / mL, 0.06-0.5μg / mL, 0.07-0.5μg / mL, 0.08-0.5μg / mL , 0.09-0.5μg / mL, 0.1-0.5μg / mL, 0.15-0.5μg / mL, 0.2-0.5μg / mL, 0.25-0. 5μg / mL, 0.3-0.5μg / mL, 0.35-0.5μg / mL, 0.4-0.5μg / mL, 0.005-0.4μg / mL,0.01-0.4μg / mL, 0.02-0.4μg / mL, 0.03-0.4μg / mL, 0.04-0.4μg / mL, 0.05-0.4μg / mL, 0.06-0.4μg / m L, 0.07-0.4μg / mL, 0.08-0.4μg / mL, 0.09-0.4μg / mL, 0.1-0.4μg / mL, 0.15-0.4μg / mL, 0.2-0.4μg / mL , 0.25-0.4μg / mL, 0.3-0.4μg / mL, 0.35-0.4μg / mL, 0.005-0.2μg / mL, 0.01-0.2μg / mL, 0.02-0.2μg / mL mL, 0.03-0.2μg / mL, 0.04-0.2μg / mL, 0.05-0.2μg / mL, 0.06-0.2μg / mL, 0.07-0.2μg / mL, 0.08-0.2μg / mL, 0.09-0.2μg / mL, 0.1-0.2μg / mL, 0.15-0.2μg / mL, 0.005-0.1μg / mL, 0.01-0.1μg / mL, 0.02-0.1μg / mL, 0.03-0.1μg / mL, 0.04-0.1μg / mL, 0.05-0.1μg / mL, 0.06-0.1μg / mL, 0.07-0.1μg / mL, 0.08-0. The concentration of cyanocobalamin may be 1 μg / mL, 0.09 to 0.1 μg / mL, 0.005 to 0.09 μg / mL, 0.01 to 0.09 μg / mL, 0.02 to 0.09 μg / mL, 0.03 to 0.09 μg / mL, 0.04 to 0.09 μg / mL, 0.05 to 0.09 μg / mL, 0.06 to 0.09 μg / mL, 0.07 to 0.09 μg / mL, or 0.08 to 0.09 μg / mL. The concentration of cyanocobalamin is preferably 0.02 to 0.4 μg / mL, more preferably 0.04 to 0.4 μg / mL.

[0070] In the stabilization method of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" described in the section "1. Beverages containing cobalamin and carnosine" can be used.

[0071] 4. Method for Improving the Aftertaste of Carnosine One aspect of the present invention provides the following method for improving the aftertaste of carnosine (hereinafter also referred to as the "aftertaste improving method of the present invention"). According to one aspect of the present invention, there is provided a method for improving the aftertaste of carnosine, which comprises adding at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin to a concentration of 0.005 to 1 μg / mL in a solution containing 0.01 to 15 mg / mL of carnosine.

[0072] The inventors have surprisingly found that the undesirable taste of carnosine can be reduced by adjusting the content of carnosine and the at least one cobalamin within a specific range.

[0073] In one embodiment of the aftertaste improving method of the present invention, the concentration of carnosine is 0.01 to 15 mg / mL. In another embodiment, the concentration of carnosine is 0.05 to 15 mg / mL, 0.10 to 15 mg / mL, 0.20 to 15 mg / mL, 0.30 to 15 mg / mL, 0.40 to 15 mg / mL, 0.50 to 15 mg / mL, 0.60 to 15 mg / mL, 0.70 to 15 mg / mL, 0.75 to 15 mg / mL, 0.80 to 15 mg / mL, 0.90 to 15 mg / mL, 1 to 15 mg / mL, 1.5 to 15 mg / mL, 2 to 15 mg / mL, 2.5 to 15 mg / mL, 3 to 15 mg / mL, 3.5 to 15 mg / mL, 4 to 15 mg / mL, mL, 4.5 to 15 mg / mL, 5 to 15 mg / mL, 5.5 to 15 mg / mL, 6 to 15 mg / mL, 6.5 to 15 mg / mL, 7-15mg / mL, 7.5-15mg / mL, 8-15mg / mL, 0.01-12mg / mL, 0.05-12mg / mL, 0 .10-12mg / mL, 0.20-12mg / mL, 0.30-12mg / mL, 0.40-12mg / mL, 0.50-12m g / mL, 0.60-12 mg / mL, 0.70-12 mg / mL, 0.75-12 mg / mL, 0.80-12 mg / mL, 0. 90-12mg / mL, 1-12mg / mL, 1.5-12mg / mL, 2-12mg / mL, 2.5-12mg / mL, 3-12 mg / mL, 3.5-12 mg / mL, 4-12 mg / mL, 4.5-12 mg / mL, 5-12 mg / mL, 5.5-12 mg / mL mL, 6-12 mg / mL, 6.5-12 mg / mL, 7-12 mg / mL, 7.5-12 mg / mL, 8-12 mg / mL, 0. 01-10mg / mL, 0.05-10mg / mL, 0.10-10mg / mL, 0.20-10mg / mL, 0.30-10mg / mL, 0.40-10mg / mL, 0.50-10mg / mL, 0.60-10mg / mL, 0.70-10mg / mL, 0.75-10mg / mL, 0.80-10mg / mL, 0.90-10mg / mL, 1-10mg / mL, 1.5-10mg / mL, 2-10mg / mL, 2.5-10mg / mL, 3-10mg / mL, 3.5-10mg / mL, 4-10mg / mL, 4.5-10mg / mL, 5-10mg / mL, 5.5-10mg / mL, 6-10mg / mL, 6.5-10mg / mL, 7-10mg / mL,7.5~10mg / mL、8~10mg / mL、0.01~8mg / mL、0.05~8mg / mL、0.10~8mg / mL、0.20~8mg / mL、0.30~8mg / mL、0.40~8mg / mL、0.50~8mg / mL、0.60~8mg / mL、0.70~8mg / mL、0.75~8mg / mL、0.80~8mg / mL、0.90~8mg / mL、1~8mg / mL、1.5~8mg / mL、2~8mg / mL、2.5~8mg / mL、3~8mg / mL、3.5~8mg / mL、4~8mg / mL、4.5~8mg / mL、5~8mg / mL、5.5~8mg / mL、6~8mg / mL、6.5~8mg / mL、7~8mg / mL、7.5~8mg / mL、0.01~7mg / mL、0.05~7mg / mL、0.10~7mg / mL、0.20~7mg / mL、0.30~7mg / mL、0.40~7mg / mL、0.50~7mg / mL、0.60~7mg / mL、0.70~7mg / mL、0.75~7mg / mL、0.80~7mg / mL、0.90~7mg / mL、1~7mg / mL、1.5~7mg / mL、2~7mg / mL、2.5~7mg / mL、3~7mg / mL、3.5~7mg / mL、4~7mg / mL、4.5~7mg / mL、5~7mg / mL、5.5~7mg / mL、6~7mg / mL、6.5~7mg / mL、0.01~6mg / mL、0.05~6mg / mL、0.10~6mg / mL、0.20~6mg / mL、0.30~6mg / mL、0.40~6mg / mL、0.50~6mg / mL、0.60~6mg / mL、0.70~6mg / mL、0.75~6mg / mL、0.80~6mg / mL、0.90~6mg / mL、1~6mg / mL、1.5~6mg / mL、2~6mg / mL、2.5~6mg / mL、3~6mg / mL、3.5~6mg / mL、4~6mg / mL、4.5~6mg / mL、5~6mg / mL、5.5~6mg / mL、6~6mg / mL、6.5~6mg / mL、7~6mg / mL、7.5~6mg / mL、8~6mg / mL、0.01~4mg / mL、0.05~4mg / mL、0.10~4mg / mL、0.20~4mg / mL、0.30~4mg / mL、0.40~4mg / mL、0.50~4mg / mL、0.60~4mg / mL、0.70~4mg / mL、0.75~4mg / mL、0.80~4mg / mL、0.90-4mg / mL, 1-4mg / mL, 1.5-4mg / mL, 2-4mg / mL, 2.5-4mg / mL, 3-4mg / mL, 3.5-4mg / mL, 0.01-3mg / mL, 0.05-3mg / mL, 0.10-3mg / mL , 0.20-3mg / mL, 0.30-3mg / mL, 0.40-3mg / mL, 0.50-3mg / mL, 0.60-3mg / mL, 0.70-3mg / mL, 0.75-3mg / mL, 0.80-3mg / mL, 0.90-3mg / m The carnosine concentration may be 0.40-7 mg / mL or 0.50-4 mg / mL. The stabilization method according to one embodiment of the present invention includes adding carnosine to the above-mentioned concentrations.

[0074] When adding at least one type of cobalamin to a solution containing carnosine, the at least one type of cobalamin may be obtained by extraction with an aqueous solvent from a material containing the at least one type of cobalamin (e.g., seafood (especially clams and short-necked clams) or animal meat (e.g., chicken liver)), or may be obtained by chemical synthesis. Alternatively, the at least one type of cobalamin may be added by adding a food ingredient containing the at least one type of cobalamin to the beverage.

[0075] In one embodiment of the aftertaste improving method of the present invention, the at least one cobalamin is contained at a concentration of 0.005 to 1 μg / mL. According to another embodiment, the at least one cobalamin is contained at a concentration of, for example, 0.01 to 1 μg / mL, 0.02 to 1 μg / mL, 0.03 to 1 μg / mL, 0.04 to 1 μg / mL, 0.05 to 1 μg / mL, 0.06 to 1 μg / mL, 0.07 to 1 μg / mL, 0.08 to 1 μg / mL, 0.09 to 1 μg / mL, 0.1 to 1 μg / mL, 0.15 to 1 μg / mL, 0.2 to 1 μg / mL, 0.25 to 1 μg / mL, 0.3 to 1 μg / mL, 0.35 to 1 μg / mL, 0.4 to 1 μg / mL, 0.00 5-0.8μg / mL, 0.01-0.8μg / mL, 0.02-0.8μg / mL, 0.03-0.8μg / mL, 0.04- 0.8μg / mL, 0.05-0.8μg / mL, 0.06-0.8μg / mL, 0.07-0.8μg / mL, 0.08-0.8 μg / mL, 0.09-0.8 μg / mL, 0.1-0.8 μg / mL, 0.15-0.8 μg / mL, 0.2-0.8 μg / m L, 0.25-0.8μg / mL, 0.3-0.8μg / mL, 0.35-0.8μg / mL, 0.4-0.8μg / mL, 0.0 05-0.6μg / mL, 0.01-0.6μg / mL, 0.02-0.6μg / mL, 0.03-0.6μg / mL, 0.04 ~0.6μg / mL, 0.05~0.6μg / mL, 0.06~0.6μg / mL, 0.07~0.6μg / mL, 0.08~0 .6μg / mL, 0.09-0.6μg / mL, 0.1-0.6μg / mL, 0.15-0.6μg / mL, 0.2-0.6μg / mL, 0.25-0.6μg / mL, 0.3-0.6μg / mL, 0.35-0.6μg / mL, 0.4-0.6μg / mL, 0 .. 005-0.5μg / mL, 0.01-0.5μg / mL, 0.02-0.5μg / mL, 0.03-0.5μg / mL, 0.0 4-0.5μg / mL, 0.05-0.5μg / mL, 0.06-0.5μg / mL, 0.07-0.5μg / mL, 0.08- 0.5μg / mL, 0.09-0.5μg / mL, 0.1-0.5μg / mL, 0.15-0.5μg / mL, 0.2-0.5μ g / mL, 0.25 to 0.5 μg / mL, 0.3 to 0.5 μg / mL, 0.35 to 0.5 μg / mL, 0.4 to 0.5 μg / mL,0.005 to 0.4 μg / mL, 0.01 to 0.4 μg / mL, 0.02 to 0.4 μg / mL, 0.03 to 0.4 μg / mL, 0.04 to 0.4 μg / mL, 0.05 to 0.4 μg / mL, 0.06 to 0.4 μg / mL, 0.07-0.4μg / mL, 0.08-0.4μg / mL, 0.09-0.4μg / mL, 0.1-0.4μg / mL, 0.15-0.4μg / mL, 0.2-0.4μg / mL, 0.25-0.4μg / mL, 0.3- The concentration of the at least one cobalamin may be 0.4 μg / mL, 0.35-0.4 μg / mL, 0.005-0.2 μg / mL, 0.01-0.2 μg / mL, 0.02-0.2 μg / mL, 0.03-0.2 μg / mL, 0.04-0.2 μg / mL, 0.05-0.2 μg / mL, 0.06-0.2 μg / mL, 0.07-0.2 μg / mL, 0.08-0.2 μg / mL, 0.09-0.2 μg / mL, 0.1-0.2 μg / mL, or 0.15-0.2 μg / mL. The concentration of the at least one cobalamin is preferably 0.02-0.8 μg / mL, more preferably 0.05-0.6 μg / mL. This concentration refers to the concentration of the cobalamin contained in the beverage if it contains only one type of cobalamin, and refers to the total concentration of the cobalamins contained in the beverage if it contains two or more types of cobalamin.

[0076] In one aspect of the present invention, the concentration when containing cyanocobalamin is not limited, for example, it may be 0.00~1μg / mL, 0.01~1μg / mL, 0.02~1μg / mL, 0.03~1μg / mL, 0.04~1μg / mL. 05~1μg / mL、0.06~1μg / mL、0.07~1μg / mL、0.08~1μg / mL、0.09~1μg / mL、0.1~1μg / mL L、0.4~1μg / mL、0.005~0.8μg / mL、0.01~0.8μg / mL、0.02~0.8μg / mL、0.03~0 .8μg / mL、0.04~0.8μg / mL、0.05~0.8μg / mL、0.06~0.8μg / mL、0.07~0.8μg / m L、0.08~0.8μg / mL、0.09~0.8μg / mL、0.1~0.8μg / mL、0.15~0.8μg / mL、0.2~0 .8μg / mL、0.25~0.8μg / mL、0.3~0.8μg / mL、0.35~0.8μg / mL、0.4~0.8μg / mL、0. . .6μg / mL、0.05~0.6μg / mL、0.06~0.6μg / mL、0.07~0.6μg / mL、0.08~0.6μg / m L、0.09~0.6μg / mL、0.1~0.6μg / mL、0.1~0.6μg / mL、0.2~0.6μg / mL、0.2~0. . 0. .5μg / mL、0.06~0.5μg / mL、0.07~0.5μg / mL、0.08~0.5μg / mL、0.09~0.5μg / m L、0.1~0.5μg / mL、0.15~0.5μg / mL、0.2~0.5μg / mL、0.25~0.5μg / mL、0.2~0... 5μg / mL、0.35~0.5μg / mL、0.4~0.5μg / mL、0.005~0.4μg / mL、0.01~0..μg / mL、0.02-0.4μg / mL, 0.03-0.4μg / mL, 0.04-0.4μg / mL, 0.05-0.4μg / mL, 0.06-0.4μg / mL, 0.07-0.4μg / mL, 0.08-0.4 μg / mL, 0.09-0.4 μg / mL, 0.1-0.4 μg / mL, 0.15-0.4 μg / mL, 0.2-0.4 μg / mL, 0.25-0.4 μg / mL, 0.3-0.4 μg / mL, 0.35-0 The concentration of cyanocobalamin may be 0.4 μg / mL, 0.005 to 0.2 μg / mL, 0.01 to 0.2 μg / mL, 0.02 to 0.2 μg / mL, 0.03 to 0.2 μg / mL, 0.04 to 0.2 μg / mL, 0.05 to 0.2 μg / mL, 0.06 to 0.2 μg / mL, 0.07 to 0.2 μg / mL, 0.08 to 0.2 μg / mL, 0.09 to 0.2 μg / mL, 0.1 to 0.2 μg / mL, or 0.15 to 0.2 μg / mL. The concentration of cyanocobalamin is preferably 0.02 to 0.8 μg / mL, more preferably 0.05 to 0.6 μg / mL.

[0077] In the aftertaste improving method of the present invention, the "cobalamin," "carnosine," "chicken extract," "propylene glycol," and "other ingredients" may be those described in the section "1. Beverages containing cobalamin and carnosine."

[0078] 5. Cobalamin Decomposition Inhibitor One aspect of the present invention provides a cobalamin degradation inhibitor (hereinafter also referred to as the "decomposition inhibitor of the present invention"). According to one aspect of the present invention, there is provided an inhibitor of the degradation of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, which comprises carnosine.

[0079] The degradation inhibitor according to one embodiment of the present invention consists essentially of carnosine. This means that the degradation inhibitor according to one embodiment of the present invention may contain small amounts of components other than carnosine. For example, when the degradation inhibitor consists essentially of carnosine, the carnosine content is preferably 85 to 100 wt %, more preferably 90 to 100 wt %, and even more preferably 95 to 100 wt %, based on the total weight of the degradation inhibitor. A carnosine content of 100 wt % based on the total weight of the degradation inhibitor means that the degradation inhibitor is composed of carnosine, and the content of any other components is an impurity amount (less than 1 wt %).

[0080] The degradation inhibitor according to one embodiment of the present invention may contain, as other components, various components that can be orally ingested by humans, such as a solvent such as water, a bulking agent, or a filler.

[0081] The form of the decomposition inhibitor according to one embodiment of the present invention is not particularly limited, and may be either liquid or solid.

[0082] As used herein, the term "at least" means that the number of a particular item may be equal to or greater than the recited number. Also, as used herein, the term "about" means that the subject lies within a range of ±25%, ±10%, ±5%, ±3%, ±2%, or ±1% of the numerical value following "about." For example, "about 10" means a range of 7.5 to 12.5.

[0083] The present invention will be specifically described below by showing examples, but the present invention is not limited to the following examples.

[0084] Example 1-1: Evaluation of the stability of cyanocobalamin Using carnosine (catalog No. 9625, Sigma-Aldrich USA, purity ≥ 99%) and cyanocobalamin (catalog No. 0429155, DSM Netherlands, purity ≥ 96%), an 80 mg / mL carnosine standard solution and a 2 mg / mL cyanocobalamin standard solution were prepared, respectively. These standard solutions were diluted to prepare 10x stock solutions for the preparation of experimental samples.

[0085] For each experimental sample, 45 carnosine-cyanocobalamin-containing samples listed in Table 1 were prepared by mixing a 10x carnosine stock solution with a 10x cyanocobalamin stock solution and diluting appropriately with tap water. Similarly, samples with the same composition as samples 1, 10, 19, 28, and 37 were prepared as control samples (without retort sterilization). Note that the carnosine used in the test was 99% or higher in purity. If the impurities in a sample containing 8 mg / mL of carnosine were assumed to be entirely propylene glycol, the maximum propylene glycol content would be approximately 0.08 mg / mL (approximately 80 ppm). Furthermore, since the purity of cyanocobalamin was 96% or higher, if the impurities in a sample containing 0.4 μg / mL of cyanocobalamin were assumed to be entirely propylene glycol, the maximum propylene glycol content would be approximately 0.016 μg / mL (approximately 0.016 ppm).

[0086] All experimental samples were subjected to heat treatment in an autoclave at 120°C for 8 minutes, conditions that simulate a retort sterilization process, and then cooled to ambient temperature for 2 hours before analysis.

[0087] The amount of cyanocobalamin contained in each heat-treated sample was determined by LC-MSMS under the following conditions. A known method was used for the measurement, and an Agilent 1290 Binary LC equipped with an AB Sciex 5600 and a triple TOF system was used as the instrument. A CORTECS T3 column (120 Å, 1.6 μm, 2.1 mm × 100 mm (Waters)) was used as the liquid chromatograph column. Other conditions were as follows. Injected sample volume: 5 μL Mobile phase: Solution A: Water containing 0.1% formic acid Solution B: Acetonitrile containing 0.1% formic acid Column temperature: 40°C Flow rate: 0.4 mL / min Gradient (concentration of solution B) 0 to 1 min: Concentration of solution B 1% 1 to 7.5 min: Concentration of solution B 1% → 40% 7.5 to 10 min: Concentration of solution B 40% → 100% 10 to 12.5 min: Concentration of solution B 100% → 100% 12.5 to 13 min: Concentration of solution B 100% → 1% 13 to 15 min: Concentration of solution B 1%

[0088] The measurement results are shown in Figure 1 and Tables 3 and 4. Table 3 summarizes the actual cyanocobalamin content measured after the retort test in the presence of carnosine. Table 4 shows the percentage of cyanocobalamin contained in each experimental sample, with the amount of cyanocobalamin contained in the control sample (unheat-treated) set at 100. Because the control sample was not subjected to heat treatment, it is assumed that the cyanocobalamin contained in the control sample was not reduced by thermal decomposition. Therefore, the percentage of cyanocobalamin contained in each experimental sample shown in Table 4 can be considered equivalent to the retention rate of cyanocobalamin after heat treatment.

[0089]

[0090] Furthermore, to verify the stabilizing effect of carnosine on cyanocobalamin, the obtained data are summarized in Figure 2. Figure 2 shows the average data for experimental samples containing cyanocobalamin at 0.005 μg / mL, 0.04 μg / mL, 0.05 μg / mL, 0.1 μg / mL, and 0.4 μg / mL of carnosine concentration.

[0091] Example 1-2: Evaluation of Cyanocobalamin Stability in a Chicken Extract-Containing Beverage Cyanocobalamin (Catalog No. 0429155, manufactured by DSM Netherlands, purity ≥ 96%) and chicken extract (manufactured by Suntory Beverage and Food Thailand, carnosine content: 0.77 g / L) were used to prepare a sample containing 0.110 μg / mL of cyanocobalamin (11.0 μg / 100 mL) and 0.77 mg / mL of carnosine. The resulting chicken soup beverage was subjected to heat treatment in the same manner as in Example 1-1. The cyanocobalamin concentrations in the chicken soup beverage before and after heat treatment were measured in the same manner as in Example 1-1. The cyanocobalamin concentration in the chicken soup beverage before heat treatment was 10.9 μg / 100 mL, and the cyanocobalamin concentration after heat treatment was 11.02 μg / 100 mL. From this, it can be understood that almost 100% of the cyanocobalamin was retained before and after heat treatment.

[0092] Example 2: Sensory Evaluation of Carnosine Carnosine (Catalog No. 9625, Sigma-Aldrich USA, purity 99% or higher) and cyanocobalamin (Catalog No. 0429155, DSM Netherlands, purity 96% or higher) were added to water to prepare Samples A to J so as to obtain the compositions shown in Table 5. In addition, as control samples, samples A to J that did not contain cyanocobalamin were also prepared.

[0093] The taste of the sample beverages obtained using the above procedure was evaluated. Each sample was evaluated by four expert panelists based on the following criteria. [Sensory evaluation criteria] Rating Criteria 6 Aftertaste is greatly improved. 5 Aftertaste is greatly improved. 4 Aftertaste is improved. 3 Aftertaste is slightly improved. 2 Aftertaste is only slightly improved. 1 Aftertaste is slightly improved. 0 No difference from the control sample.

[0094] Before conducting the test, the panelists thoroughly discussed the above evaluation criteria. The results are summarized in Table 5. The values ​​of the sensory evaluation results in the table below are the average scores of the four panelists.

Claims

1. A packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.70 to 15 mg / mL, and the content of the at least one cobalamin is 0.005 to 1 μg / mL.

2. The packaged beverage according to claim 1, which contains chicken extract.

3. The packaged beverage according to claim 1 or 2, wherein the at least one cobalamin comprises cyanocobalamin.

4. A packaged beverage according to any one of claims 1 to 3, having a carnosine content of 0.75 to 7 mg / mL.

5. A packaged beverage according to any one of claims 1 to 4, having a carnosine content of 0.80 to 4 mg / mL.

6. A bottled beverage according to any one of claims 1 to 5, wherein the content of the at least one type of cobalamin is 0.04 to 0.4 μg / mL.

7. A bottled beverage according to any one of claims 1 to 6, wherein the weight ratio of the content of carnosine to the content of the at least one type of cobalamin is 500:1 to 50,000:

1.

8. A bottled beverage according to any one of claims 1 to 7, wherein after a retort test at 120°C for 8 minutes, 80% by weight or more of the at least one type of cobalamin is maintained relative to the content of the at least one type of cobalamin before the retort test.

9. The packaged beverage according to any one of claims 1 to 8, which is a ready-to-drink (RTD) beverage, a soft drink, a chicken soup, a jelly-like drink or a functional drink.

10. A method for producing the packaged beverage according to any one of claims 1 to 9, comprising adding at least one of the at least one cobalamin and carnosine to a beverage base.

11. A method for stabilizing at least one type of cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, comprising adding carnosine to a solution containing the at least one type of cobalamin at a concentration of 0.01 to 15 mg / mL.

12. A method for improving the aftertaste of carnosine, comprising adding at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin to a solution containing 0.01 to 15 mg / mL of carnosine to give a concentration of 0.005 to 1 μg / mL.

13. A packaged beverage containing carnosine and at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin, and sulfitocobalamin, wherein the carnosine content is 0.50 to 15 mg / mL, the content of the at least one cobalamin is 0.005 to 1 μg / mL, and the propylene glycol content is less than 0.3 mg / mL.

14. The packaged beverage according to any one of claims 1 to 9 and 13, having an ethanol content of less than 0.001 mg / mL.

15. An inhibitor of the degradation of at least one cobalamin selected from the group consisting of cyanocobalamin, hydroxocobalamin, adenosylcobalamin, methylcobalamin and sulfitocobalamin, including carnosine.

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