Nutritional composition capable of improving bioavailability of aromatic amino acid and use thereof

Abstract

A nutritional composition capable of improving the bioavailability of an aromatic amino acid and a use thereof. The composition comprises: a protein, taurine, and choline in a mass ratio of 1:(0.0019-0.01):(0.008-0.02). Also provided are food containing the composition, and a use of the composition or food in the preparation of a product for improving the bioavailability of an aromatic amino acid.

Description

Nutritional composition for improving bioavailability of aromatic amino acids and application thereof TECHNICAL FIELD

[0001] The present application belongs to the technical field of nutritional composition, and particularly relates to a nutritional composition for improving bioavailability of aromatic amino acids and related application. BACKGROUND

[0002] Aromatic amino acids refer to amino acids with benzene ring structure in molecular structure, mainly including tyrosine, phenylalanine and tryptophan. Aromatic amino acids play an important role in life activities. In a study on children born small for gestational age (SGA), the correlation between the content of aromatic amino acids in the blood of full-term children and full-term SGA and their growth and development was analyzed, and the results showed that appropriate supplementation of aromatic amino acids in the diet of full-term SGA was beneficial to the growth and development of full-term SGA.

[0003] Tyrosine is a semi-essential amino acid required for the growth and development of infants, which can be synthesized in the body but can only be produced by hydroxylation of phenylalanine. Tyrosine and phenylalanine together synthesize important neurotransmitters and hormones, and participate in body glucose metabolism and fat metabolism. Tyrosine is an important raw material for the formation of adrenaline, norepinephrine, thyroid hormone, melanin and other hormones and functional substances. Among them, thyroid hormone is one of the important factors for maintaining normal metabolism of blood lipids and affecting protein synthesis, and the change of thyroid function is closely related to the metabolism of other substances in the body (such as glucose, protein, fat and water and electrolyte balance).

[0004] Phenylalanine is an essential amino acid for the growth and development of infants, which is involved in protein synthesis and closely related to the metabolism and health of infants. Phenylalanine can be metabolized into tyrosine, and phenylalanine hydroxylation is an important step to eliminate excess phenylalanine in the body and provide tyrosine. However, compared with adults, the activity of phenylalanine-4-monooxygenase in infants is lower, and the metabolic conversion capacity of phenylalanine is limited, so it is very important to provide appropriate amount of phenylalanine for the growth and development of infants.

[0005] Tryptophan is another important aromatic amino acid, which is not only an essential amino acid for protein synthesis, but also an important pathway for serotonin metabolism and kynurenine metabolism. The metabolites of the serotonin pathway are serotonin and melatonin, both of which are involved in almost all physiological activities, including appetite regulation, digestion, circadian rhythm, cognition, learning and memory, etc. Tryptophan can generate kynurenine, nicotinic acid and nicotinamide adenine dinucleotide (NAD) through the kynurenine metabolic pathway. Research data shows that tryptophan in the diet can regulate the sleep pattern of infants and also affect the early brain development, especially the neuron circuit and neuron plasticity, etc.

[0006] It is crucial to provide infants with sufficient amount and variety of amino acids for their growth, because the rate of protein synthesis in the body is limited by the limiting essential amino acid in the diet. Previous studies have shown that the first limiting amino acid in infant formula is tryptophan, and the second and third limiting amino acids are phenylalanine and tyrosine. On the other hand, excessive intake of amino acids is also a burden on the immature kidneys and liver of infants. How to meet the needs of infants for amino acids while reducing the protein concentration has been a challenge for infant formula.

[0007] The difference in amino acid composition between cow milk protein and breast milk is large, which makes the protein quality of infant formula with cow milk as the main protein source relatively low, and the bioavailability of amino acids relatively low. Therefore, it is necessary to adjust and optimize the formula development of infant formula to improve the protein quality, improve the bioavailability of amino acids, and promote the absorption of amino acids by infants. SUMMARY

[0008] One object of the present application is to provide a composition that can improve the bioavailability of aromatic amino acids.

[0009] Another object of the present application is to provide a product, such as a food, comprising the composition.

[0010] Another object of the present application is to provide related applications of the composition or food in improving the bioavailability of aromatic amino acids.

[0011] In one aspect, the present application provides a composition comprising: protein, taurine and choline, the mass ratio of protein, taurine and choline being 1:(0.0019-0.01):(0.008-0.02).

[0012] According to some specific embodiments of the present application, in the composition of the present application, the mass ratio of protein, taurine and choline is 1:(0.0020-0.01):(0.0085-0.02).

[0013] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0021-0.009) : (0.0085-0.019).

[0014] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0022-0.009) : (0.0085-0.018).

[0015] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0023-0.009) : (0.0085-0.017).

[0016] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0024-0.0085) : (0.0085-0.016).

[0017] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0025-0.0085) : (0.010-0.015).

[0018] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0019-0.008) : (0.008-0.02).

[0019] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0020-0.008) : (0.009-0.02).

[0020] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0021-0.007) : (0.0090-0.020).

[0021] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0022-0.006) : (0.010-0.019).

[0022] According to some embodiments of the application, in the composition of the application the mass ratio of protein, taurine and choline is 1 : (0.0023-0.0055) : (0.010-0.018).

[0023] According to some embodiments of the present application, the mass ratio of protein, taurine and choline in the composition of the present application is 1 : (0.0024-0.0050) : (0.010-0.015).

[0024] According to some embodiments of the present application, the mass ratio of protein, taurine and choline in the composition of the present application is 1 : (0.0025-0.0035) : (0.010-0.015).

[0025] According to some embodiments of the present application, the protein in the composition of the present application can be plant protein and / or animal protein. In some embodiments of the present application, the protein comprises milk protein, preferably selected from bovine milk protein and / or goat milk protein. In some embodiments of the present application, the protein can be, for example, bovine milk-derived protein, including bovine milk whole protein, in particular commercially available milk protein powder or whey protein powder. In some embodiments of the present application, the whey protein content in the protein is not less than 40%, preferably not less than 60%.

[0026] According to some embodiments of the present application, the choline and taurine in the composition of the present application can be obtained from conventional commercial sources.

[0027] According to some embodiments of the present application, the composition of the present application is a nutritional composition or a food composition.

[0028] In some embodiments of the present application, the composition of the present application can mainly consist of protein, choline and taurine.

[0029] In some embodiments of the present application, the composition of the present application, in particular the nutritional composition or the food composition, can further comprise one or more of other proteins, fats, carbohydrates, vitamins, minerals, etc. in addition to the protein, choline and taurine.

[0030] In another aspect, the present application also provides use of the composition in the preparation of a product for improving the bioavailability of aromatic amino acids.

[0031] In some embodiments of the present application, the product is a nutritional product.

[0032] In some embodiments of the present application, the product is a food product.

[0033] In some embodiments of the present application, the product is a nutritional supplement, a complementary food for infants and young children, an infant formula, a follow-up formula or a special medical purpose infant formula.

[0034] In another aspect, the present application also provides a food product comprising the composition according to the present application.

[0035] According to some embodiments of the present application, the amount of the composition according to the present application (i.e. the aforementioned composition comprising protein, taurine and choline) added to the food product according to the present application can be comprised between 6 g and 36 g of protein per 100 g of dry matter of the food product.

[0036] According to some embodiments of the present application, the total protein content of the food product according to the present application can be comprised between 6 g and 36 g per 100 g of dry matter of the food product.

[0037] According to some embodiments of the present application, the taurine content of the food product according to the present application can be comprised between 20 mg and 86 mg per 100 g of dry matter of the food product.

[0038] According to some embodiments of the present application, the taurine content of the food product according to the present application can be comprised between 20 mg and 65 mg per 100 g of dry matter of the food product.

[0039] According to some embodiments of the present application, the taurine content of the food product according to the present application can be comprised between 25 mg and 55 mg per 100 g of dry matter of the food product.

[0040] According to some embodiments of the present application, the taurine content of the food product according to the present application can be comprised between 25 mg and 45 mg per 100 g of dry matter of the food product.

[0041] According to some embodiments of the present application, the taurine content of the food product according to the present application can be comprised between 25 mg and 40 mg per 100 g of dry matter of the food product.

[0042] According to some embodiments of the present application, the choline content of the food product according to the present application can be comprised between 90 mg and 200 mg per 100 g of dry matter of the food product.

[0043] According to some embodiments of the present application, the choline content of the food product according to the present application can be comprised between 100 mg and 200 mg per 100 g of dry matter of the food product.

[0044] According to some embodiments of the present application, the choline content of the food product according to the present application can be comprised between 110 mg and 200 mg per 100 g of dry matter of the food product.

[0045] According to some embodiments of the present application, the choline content of the food product according to the present application can be comprised between 110 mg and 190 mg per 100 g of dry matter of the food product.

[0046] According to some embodiments of the present application, the choline content of the food product according to the present application can be comprised between 120 mg and 150 mg per 100 g of dry matter of the food product.

[0047] In some embodiments of the present application, the food product is a milk powder or a liquid milk.

[0048] In some embodiments of the present application, the food product is an infant formula, a baby formula or a baby formula for special medical purposes.

[0049] In some embodiments of the present application, the food product of the present application is a milk powder, which can be prepared by adding / adjusting protein, taurine and choline in a base formula powder. The content of protein, taurine and choline in the milk powder of the present application can be determined by using conventional methods in the art, such as high performance liquid chromatography detection analysis technique, after the milk powder of the present application is prepared. The base formula powder is not particularly limited in the present application, as long as it is a commonly used formula of milk powder in the art. For example, the base formula powder can be a general infant formula powder or a baby formula powder. As a specific example, the base formula powder of the present application can use milk powder of animal origin as the main raw material, and can further add one or more of whey powder, vitamins, minerals, etc. As the milk powder of animal origin, cow milk powder or goat milk powder can be used.

[0050] The amount of each substance in the food product of the present application should meet the requirements of the relevant standards.

[0051] On the other hand, the present application also provides the use of the food product in the preparation of a product for improving the bioavailability of aromatic amino acids.

[0052] According to specific embodiments of the present application, in the present application, the aromatic amino acid includes one or more of tyrosine, phenylalanine and tryptophan; preferably includes one or more of tyrosine and tryptophan.

[0053] According to specific embodiments of the present application, in the present application, the improvement of the bioavailability of aromatic amino acids includes improving the digestibility and / or absorption utilization of one or more of tyrosine, phenylalanine and tryptophan. Preferably, the improvement of the bioavailability of aromatic amino acids includes improving the digestibility and / or absorption utilization of one or more of tyrosine and tryptophan.

[0054] In another aspect, the present application also provides a method for improving the bioavailability of aromatic amino acids in a protein-containing food, which comprises controlling the contents of protein, taurine and choline in the food so that the mass ratio of protein, taurine and choline is 1:(0.0019-0.01):(0.008-0.02). Preferably, the mass ratio of protein, taurine and choline is controlled within the more specific ranges of the present application, i.e., preferably the mass ratio of protein, taurine and choline is controlled to be any one of 1:(0.0020-0.01):(0.0085-0.02), 1:(0.0021-0.009):(0.0085-0.019), 1:(0.0022-0.009):(0.0085-0.018), 1:(0.0023-0.009):(0.0085-0.017), 1:(0.0024-0.0085):(0.0085-0.016), 1:(0.0025-0.0085):(0.010-0.015), 1:(0.0019-0.008):(0.008-0.02), 1:(0.0020-0.008):(0.009-0.02), 1:(0.0021-0.007):(0.0090-0.020), 1:(0.0022-0.006):(0.010-0.019), 1:(0.0023-0.0055):(0.010-0.018), 1:(0.0024-0.0050):(0.010-0.015) or 1:(0.0025-0.0035):(0.010-0.015).

[0055] In some embodiments of the present application, the effect of the composition of the present application on the bioavailability of aromatic amino acids was investigated in a piglet model experiment. Firstly, the body weight change of piglets was tracked to confirm that the test formula had no effect on the growth and development of piglets. Then the amino acid composition of the test groups was determined, and there was no significant difference in aromatic amino acids among the four test formulas. Subsequently, a 15-day feeding experiment was carried out, and after the experiment, the ileum chyme samples were collected to avoid the influence of intestinal microorganisms on the determination. After analyzing the amino acid composition of the chyme samples, the apparent and standard digestibility were calculated, which confirmed that the composition of the present application could improve the digestion and absorption rate of aromatic amino acids, especially tryptophan and tyrosine.

[0056] The composition of the present application containing protein, taurine and choline can improve the digestion and absorption of protein by infants and young children, especially can improve the digestion rate and / or absorption utilization rate of aromatic amino acids, which is beneficial to the healthy growth of infants and young children. BRIEF DESCRIPTION OF DRAWINGS

[0057] Figure 1 shows the body weight change of experimental animals in different groups in the embodiments of the present application.

[0058] Figure 2 shows the determination of the acid aromatic amino acid composition of experimental animals in different groups in the embodiments of the present application.

[0059] Figure 3 shows the apparent protein digestibility of experimental animals in different groups in the embodiments of the present application.

[0060] Figure 4 shows the determination of the apparent aromatic amino acid digestibility of experimental animals in different groups in the embodiments of the present application. In the figure, two groups indicated with the same letter represent no statistical difference between the groups, and the indicated letters without the same letter represent significant difference between the groups.

[0061] Figure 5 shows the determination of the apparent aromatic amino acid (combined) digestibility of experimental animals in different groups in the embodiments of the present application. In the figure, two groups indicated with the same letter represent no statistical difference between the groups, and the indicated letters without the same letter represent significant difference between the groups.

[0062] Figure 6 shows the determination of the standard aromatic amino acid digestibility of experimental animals in different groups in the embodiments of the present application. In the figure, two groups indicated with the same letter represent no statistical difference between the groups, and the indicated letters without the same letter represent significant difference between the groups.

[0063] Figure 7 shows the determination of the standard aromatic amino acid (combined) digestibility of experimental animals in different groups in the embodiments of the present application. In the figure, two groups indicated with the same letter represent no statistical difference between the groups, and the indicated letters without the same letter represent significant difference between the groups. DETAILED DESCRIPTION

[0064] Before further describing the embodiments of the present application, it should be understood that the scope of protection of the present application is not limited to the specific embodiments described below; it should also be understood that the terms used in the embodiments of the present application are used to describe the specific embodiments, and are not intended to limit the scope of protection of the present application.

[0065] When the embodiments give numerical ranges, it should be understood that, unless otherwise stated by the present application, each numerical range has two endpoints and any number between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present application have the same meaning as generally understood by those skilled in the art. In addition to the specific methods, devices, materials used in the embodiments, any method, device and material of the prior art similar or equivalent to those described in the embodiments of the present application can also be used to implement the present application according to the mastery of the prior art by those skilled in the art and the description of the present application.

[0066] Unless otherwise stated, the experimental methods, detection methods, preparation methods disclosed in the present application all use conventional techniques in the art.

[0067] In the examples, the protein used is derived from bovine milk whey protein powder, wherein the whey protein content is > 60%.

[0068] In the examples, the taurine and choline used are routinely commercially available.

[0069] Experiment of the effect of the nutritional composition on improving the bioavailability of aromatic amino acids

[0070] The 7-day-old piglets were selected, with an initial body weight of 2.8-3.1 kg. According to the principle of similar average weight, the piglets were randomly divided into 4 groups, with 6 piglets in each group. Each group was fed for 15 days, and the feeding amount was 42 g of dry matter per kg of body weight. The piglet's food intake, weight gain, and whether there were adverse reactions such as diarrhea were observed. On the 15th day of the experiment, the piglets were sacrificed, and the chyme samples at the end of the ileum were collected.

[0071] The piglets were individually placed in special feeding boxes, with a temperature control of 26-28°C and a relative humidity control of 40-70%. Artificial lighting was provided with 12 hours of light and dark alternation.

[0072] In addition to the 4 test groups, a nitrogen-free diet group was also set up, consisting of 5 7-day-old piglets with an initial body weight of 2.8-3.0 kg. The nitrogen-free diet group (main components are carbohydrates and minerals, without protein components) was set up to determine the basic endogenous loss of protein and amino acids, so as to calculate the standard digestibility of each amino acid in different groups later.

[0073] The food fed to each group was a reference infant formula prepared by adjusting the protein, taurine, and choline content of the base powder. The specific composition of the formula is shown in Table 1.

[0074] Table 1

[0075] Note: Vitamins and minerals were added according to regulatory requirements, and the content in the food of each group was consistent.

[0076] Aromatic amino acid detection:

[0077] Phenylalanine and tyrosine were detected according to the method in GB / T 5009.124-2016 "Determination of Amino Acids in Food" after hydrolysis in 6 mol / L hydrochloric acid solution for 22 h. Tryptophan was detected by chromatography according to GB / T 15400-2018 after hydrolysis of the sample with potassium hydroxide. The three aromatic amino acids in each group and the collected chyme samples were detected, and the apparent digestibility and ileal standard digestibility of the amino acids in different groups were calculated.

[0078] Data processing and statistical analysis:

[0079] Protein apparent digestibility of the feed (%) = [(total protein intake - ileal chyme protein amount) / total protein intake] x 100;

[0080] Amino acid apparent digestibility of the feed (%) = [(amount of ingested amino acid - amount of the same amino acid in ileal chyme) / amount of ingested amino acid] x 100;

[0081] Amino acid standard digestibility of the feed (%) = [(amount of ingested amino acid - amount of the same amino acid in ileal chyme + amount of the same amino acid endogenous in ileum) / amount of ingested amino acid] x 100.

[0082] It can be seen that the protein digestibility measured in the present application refers to the ratio of the protein (including peptides and amino acids produced after digestion) that can be absorbed and utilized by the digestive tract of the organism to the total amount of ingested protein; the amino acid digestibility measured in the present application refers to the proportion of the amino acid (including amino acids contained in proteins and peptides as well as free amino acids) that can be absorbed and utilized by the digestive tract of the organism. It can be considered that the protein / amino acid digestibility measured in the present application essentially refers to the bioavailability of protein / amino acid.

[0083] Experimental results:

[0084] 1) Body weight change of piglets in each group

[0085] The body weight of piglets was recorded every 3 days at a fixed time from the time of entering the group (D1) to the 15th day (D15) to track the change of body weight of piglets.

[0086] The results are shown in Figure 1. There was no significant difference in the body weight change of piglets in each group, and no adverse phenomena such as anorexia, bloating or diarrhea were observed in piglets during the 15-day feeding period, indicating that the tested formula can meet the normal growth needs of piglets.

[0087] 2) Amino acid composition determination of each group formula

[0088] The composition of tyrosine, phenylalanine and tryptophan in each group formula was determined before feeding piglets to determine the amino acid composition in different formulas before digestion.

[0089] The contents of tyrosine, phenylalanine and tryptophan in each group are shown in Figure 2. There was no significant difference in the total amount of calculated and determined amino acids in groups 1-4.

[0090] 3) Protein digestibility of each group

[0091] After piglets were fed with each group formula for 15 days, ileal chyme samples were collected, freeze-dried, ground, and the protein in each chyme sample was determined to calculate the apparent protein digestibility of each group.

[0092] Figure 3 shows the apparent digestibility of each group of proteins. The results show that the apparent digestibility of each group of proteins is about 80% and there is no statistical difference.

[0093] 4) Apparent digestibility of each group of amino acids

[0094] After the piglets were fed each group of formula for 15 days, the ileal chyme samples were collected, freeze-dried, ground, and the aromatic amino acids in each group of chyme samples were determined to calculate the apparent digestibility of aromatic amino acids in each group.

[0095] The results of the apparent digestibility of each amino acid are shown in Figure 4. There is a significant difference in the apparent digestibility of tyrosine and tryptophan in each group, with the highest apparent digestibility of tyrosine and tryptophan in group 4, followed by group 1 and group 3. Group 2 has lower digestibility of tyrosine and tryptophan than the other groups, especially the apparent digestibility of tryptophan is significantly lower than the other three groups, because it does not add taurine and choline.

[0096] Figure 5 shows the apparent digestibility of aromatic amino acids (combined), which is consistent with the trend of apparent digestibility of tyrosine, phenylalanine and tryptophan alone. The apparent digestibility of aromatic amino acids in group 4 is the highest, followed by group 1 and group 3. The digestibility of aromatic amino acids in group 2 is lower than that in other groups because it does not add taurine and choline.

[0097] 5) Standard digestibility of each group of amino acids

[0098] After calculating the apparent digestibility of aromatic amino acids in each group, combined with the determination of amino acid endogenous loss in the nitrogen-free diet group, the standard digestibility of aromatic amino acids in different groups was calculated.

[0099] The standard digestibility of each amino acid is shown in Figure 6. As with the apparent digestibility trend, the standard digestibility of aromatic amino acids tyrosine and tryptophan in group 4 and group 1 is higher than that in group 3 and group 2. Although there is no statistical difference in the standard digestibility of phenylalanine in each group, analysis of group 2 and group 4 shows a trend of statistical difference in the standard digestibility of phenylalanine (p=0.088).

[0100] Figure 7 shows the standard digestibility of aromatic amino acids (combined), which is consistent with the trend of apparent digestibility of tyrosine, phenylalanine and tryptophan alone. The apparent digestibility of aromatic amino acids in group 4 and group 1 is the highest, followed by group 3. The digestibility of aromatic amino acids in group 2 is lower than that in other groups because it does not add taurine and choline.

[0101] The above experiment can show that by controlling the content ratio of taurine and choline in the infant formula food, the bioavailability of aromatic amino acids in the food can be effectively improved, and the absorption and utilization of tryptophan and / or tyrosine can be promoted. Therefore, the composition containing protein, taurine and choline provided by the present application can be applied to infant formula food, the protein quality can be improved, the bioavailability of aromatic amino acids can be improved, so that the demand of infants for amino acids can be met while appropriately reducing the protein concentration in the infant formula food (to reduce the intake of limiting amino acids), and the burden on the immature kidney and liver of infants caused by excessive intake of amino acids can be avoided.

[0102] The above description of the embodiments is to facilitate the understanding and use of the present application by those skilled in the art. Those skilled in the art can easily make various modifications to these embodiments, and apply the general principles described herein to other embodiments without creative labor. Therefore, the present application is not limited to the above embodiments, and improvements and modifications made by those skilled in the art without departing from the scope of the present application should be within the scope of protection of the present application.

Claims

1. Use of a composition in the manufacture of a product for improving the bioavailability of aromatic amino acids, wherein, The composition comprises: protein, taurine and choline, and the mass ratio of the protein, taurine and choline is 1:(0.0019-0.01):(0.008-0.02).

2. The use according to claim 1, wherein, In the composition, the mass ratio of the protein, taurine and choline is 1:(0.0020-0.01):(0.0085-0.02).

3. The use according to claim 1, wherein, In the composition, the mass ratio of the protein, taurine and choline is 1:(0.0022-0.009):(0.0085-0.018).

4. The use according to claim 1, wherein, In the composition, the mass ratio of the protein, taurine and choline is 1:(0.0024-0.0085):(0.0085-0.016).

5. The use according to claim 1, wherein, In the composition, the mass ratio of the protein, taurine and choline is 1:(0.0025-0.0085):(0.010-0.015).

6. The use according to claim 1, wherein, The protein is plant protein and / or animal protein; Preferably, the protein is selected from cow milk protein and / or goat milk protein.

7. The use according to claim 1 or 6, wherein, The content of whey protein in the protein is not less than 40%.

8. The use according to claim 1, wherein, The product is a nutritional product.

9. The use according to claim 1, wherein, The product is a food product.

10. The use according to claim 1, wherein, The improvement of the bioavailability of aromatic amino acids includes increasing the digestibility and / or absorption utilization rate of one or more of tyrosine, phenylalanine and tryptophan; Preferably, the improvement of the bioavailability of aromatic amino acids includes increasing the digestibility and / or absorption utilization rate of one or more of tyrosine and tryptophan.

11. Use according to claim 9, wherein, The addition amount of the composition in the food is 6g-36g / 100g dry matter of the food, based on the protein therein.

12. The use according to claim 9, wherein, The food is milk powder or liquid milk.

13. The use according to claim 9, wherein, The food is infant formula, toddler formula or special medical purpose infant formula.

14. A method of improving bioavailability of aromatic amino acids in a protein-containing food product, wherein, The method comprises: Controlling the content of protein, taurine and choline in the food so that the mass ratio of the protein, taurine and choline is 1:(0.0019-0.01):(0.008-0.02).

15. A composition for improving bioavailability of aromatic amino acids, comprising: Protein, taurine and choline, and the mass ratio of the protein, taurine and choline is 1:(0.0019-0.01):(0.008-0.02); Preferably, the mass ratio of the protein, taurine and choline in the composition is 1:(0.0020-0.01):(0.0085-0.02), further preferably 1:(0.0022-0.009):(0.0085-0.018), further preferably 1:(0.0024-0.0085):(0.0085-0.016), further preferably 1:(0.0025-0.0085):(0.010-0.015). Preferably, the composition is a nutritional composition or a food composition.

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