A system providing isolated temperature control for electrochemical sensors

Abstract

The invention relates to a system that provides the calculation process as a result of a direct analysis by correlating the reaction kinetics with temperature, the isolated temperature control of electrochemical sensors suitable for use in field applications, which is used to perform a precise temperature control reaction in a closed environment.

Description

[0001] A SYSTEM PROVIDING ISOLATED TEMPERATURE CONTROL FOR ELECTROCHEMICAL SENSORS

[0002] Technical Field

[0003] The invention comprises placing an electrochemical biosensor preferably in the middle of a cap integrated into the test tube and placing a heat conductive element extending to the electrode level at which the chemical reaction takes place . Thus , precise temperature control can be conducted while the reaction takes place in a closed environment . In this direction, a calculation process can be carried out as a result of a direct analysis by correlating the reaction kinetics with temperature , or temperature control can be carried out outside the test tube thanks to the heat conductive plate , and heat trans fer related to the heat elements can be carried out to the test tube in the direction of control . As a result , it will be possible to conduct field applications with sensitive temperature control .

[0004] State of the Art

[0005] Printed electrochemical biosensors are sensors that provide an electronic output as a result of a biological reaction or interaction and allow digital measurements to be performed with devices called potentiostats . For the production of the speci fied biosensors , components such as carbon, gold, silver / silver chloride are printed on a plasti c or ceramic surface . Then, the chemical agents that will create the relevant reaction are immobili zed on the working electrode of this sensor . For the use of these sensors designed according to a speci fic analyte ; the electrodes of the sensors are integrated into the device called potentiostat . The liquid solution with the analyte is dripped onto the surface of the sensor or the sensor is immersed in a liquid . Within the relevant electrochemical method, the relevant voltage or current is applied to the sensor and electronic information is obtained for the determined reaction or interaction . Afterwards , this information is converted into concentration data, and the analysis is performed . Although these sensors have been developed for field applications , they can still only be applied in laboratory environments due to the additional chemical and process controls required .

[0006] One of the biggest problems of the speci fied sensors at the commerciali zation stage is the repeatability problem . One of the reasons for the inability to achieve repeatability is the inability to achieve accurate temperature control . Especially in field applications , there is a deviation in the results due to the di f ferent solution temperatures of the analysed solutions in winter and summer seasons . To achieve this control , additional devices and non-disposable temperature probes are needed . Even i f such an additional device is used, the probe needs to be free of inter-analysis contamination and analyte residues . For this , solvents and steri le cloths or napkins must be used . These elements must be free of enzymes that degrade RNA, DNA, and proteins . Due to the di f ficulty of these processes , field applications of the speci fied sensors cannot be conducted .

[0007] Although printed electrochemical biosensors included in the present art are planned for field applications , applications are performed for experiments performed at room temperature in laboratory environments . Therefore , no precise temperature control is performed . The systems in which temperature control is performed result from applying a drop to the analysis part of the biosensor and performing analysis or using the immersion method .

[0008] A similar system is described at https : / / www . researchgate . net / f igure / Electrochemical -cell- set ting- with- 500-mL-bath-and- 52 -mm- spacing-be tween- the - counter-and figl 334514081 . In this system, it is understood that the bottom heating is made , and a probe is immersed in the reaction vessel , and the heat is adj usted by transmitting it to the heating table accordingly . Such methods are applied in the laboratory for long-term analysis . It is not possible to use this type of system for field applications .

[0009] There are no systems suitable for field application within the current art . The solution, which allows field applications , constitutes an advantage as it allows on-site and fast analysis .

[0010] Objects of the Invention

[0011] The obj ect of the invention relates to an electrochemical biosensor associated with a cap integrated into the test tube and an isolated printed electrochemical biosensor containing a heat conductive element extending to the electrode level where the chemical reaction takes place .

[0012] The present invention comprises creating a structure that enables direct analysis from the tube by keeping the part of the printed biosensor that performs the electrochemical reaction inside the caps of the chemical tubes and the connection part on the outside .

[0013] However, it will be a heat conductive plate placed parallel to the front or back surface of the biosensor . One end of this plate will extend to the surface where the sensor reacts , and the other end i s designed to pass through the tube cap and expand on the outside of the cap . With this design, the liquid samples placed in the test tube will be removed directly from the outside world by attaching the cap containing the biosensor and the heat conductive plate and a safe test environment will be created . Thanks to the outer end of the biosensor, the electronic connection will be made , and the relevant reaction will be initiated .

[0014] However, making the reaction medium closed together with the thermal conductive plate cap helps to maintain the temperature balance .

[0015] According to the preferred embodiment of the invention, there will be a temperature probe integrated into the cylindrical heat trans fer system to be installed around the test tube . Thus , temperature trans fer will be provided from the outer surface of the closed test tube , liquid temperature will be trans ferred directly out of the cap with the thermal conductive plate integrated in the cap, and information will be provided to the heat trans fer system for heating by making precise measurement with the temperature probe adj acent to the part of the plate outside the cap . In this way, the temperature on the biosensor surface will be controlled quickly and precisely, and the biosensor measurement di f ferences that will occur according to the di f ferences in external weather conditions wil l be prevented . At the same time , it will protect from external contamination and prevent inter-analysis analyte contamination .

[0016] Description of the Figures

[0017] Figure 1 . A further view of the electrochemical sensor, Figure 2. A separate view of the heat transfer plate,

[0018] Figure 3. A view of the heat transfer block and sensor integrated cap structure,

[0019] Figure 4. A lower view of the heat transfer block and sensor integrated cap structure,

[0020] Figure 5. A side view of the tube and cap together,

[0021] Figure 6. A perspective view of the tube on the electrochemical analysis module,

[0022] Figure 7. A side view of the tube on the electrochemical analysis module,

[0023] Figure 8. A combined view of the electrochemical analysis module and the device lower module,

[0024] Figure 9. A detailed view of the heat transfer element

[0025] Description of References in Figures

[0026] A. Electrochemical sensor

[0027] B. Heat transfer plate

[0028] C. Electrochemical analysis module

[0029] D. Device upper module

[0030] 1. Working electrode

[0031] 2. Reference electrode

[0032] 3. Heat transfer plate contact point

[0033] 4. Integration section

[0034] 5. Tube cap

[0035] 6. Tube

[0036] 7. Temperature control connection cable

[0037] 8. Battery

[0038] 9. System control board

[0039] 10. Device temperature sensor

[0040] 11. Sensor connection point

[0041] 12. Device temperature system connection

[0042] 13. Heat transfer element

[0043] 14. Heating element 15 . Heat trans fer block

[0044] 16 . Resistance connection points

[0045] Description of the Invention

[0046] The invention relates to a system that provides the calculation process as a result of a direct analysis by correlating the reaction kinetics with temperature , the isolated temperature control of electrochemical sensors suitable for use in field applications , which is used to perform reactions under a precise temperature control in a closed environment .

[0047] The system comprises at least one electrochemical biosensor (A) integrated on the tube cap ( 5 ) of the test tube ( 6 ) and a heat trans fer plate (B ) extending to the electrode level where the reaction takes place .

[0048] In this direction, a calculation process can be carried out as a result of a direct analysis by correlating the reaction kinetics with temperature , or temperature control can be carried out outside the test tube thanks to the heat conductive plate , and heat trans fer related to the heat elements can be carried out to the test tube in the direction of control .

[0049] The present invention comprises creating a structure that enables direct analysis from the tube by keeping the part of the printed electrochemical biosensor (A) that performs the reaction inside the caps ( 5 ) of the chemical tubes ( 6 ) and the connection part on the outside .

[0050] Figure 2 shows the electrochemical biosensor (A) formed in the inner part of the tube cap ( 5 ) . Within this embodiment , the heat transfer plate (B) is disposed parallel to the front or rear surface of the electrochemical biosensor (A) .

[0051] The embodiment shown in Figure 2 shows the embodiment in which the tube cap (5) is removably connected to the tube (6) .

[0052] Within this embodiment, the heat transfer plate (B) is configured to extend from one end to the surface where the electrochemical biosensor (A) performs the reaction, and the other end is designed to pass through the tube cap (5) and expand on the outer side of the cap (5) . With this design, the liquid samples placed in the test tube (5) will be isolated from the external environment and a safe test environment will be created by attaching the cap (5) containing the electrochemical biosensor (A) and the heat conductive plate (B) . Thanks to the outer end of the biosensor (A) , the electronic connection will be made, and the relevant reaction will be initiated.

[0053] The electrochemical biosensor (A) is positioned in the middle of the cap (5) according to the preferred embodiment of the invention .

[0054] The electrochemical biosensor (A) according to one of the embodiments of the invention comprises at least one working electrode (1) and at least one reference electrode (2) . In this embodiment, at least one end of the electrochemical biosensor (A) is provided with the integration section (4) .

[0055] According to Figure 1, two reference electrodes (2) are positioned on both sides of the working electrode (1) .

[0056] Figure 2 shows an embodiment of the heat transfer plate (B) . Heat transfer plate (B) within this embodiment comprises at least one heat transfer plate contact point (3) .

[0057] This section is wider than the other section and connects the heat transfer plate (B) to the cap (5) .

[0058] In Figure 4, the cap (5) is shown from below. Within this embodiment, the lower part of the cap (5) comprises at least one inlet opening in order to provide connection to the integration section (4) .

[0059] After the tube (6) and the cap (5) are connected, they can be connected to an electrochemical analysis module (C) (Figure 6) . The electrochemical analysis module can be identified as the control unit of the system. The information received from the tube (6) thanks to the connections on the cap (5) is processed by this module (C) .

[0060] According to Figure 6, the electrochemical analysis module (C) is equipped with the temperature control connection cable (6) , the battery (8) , and / or the system control board (9) .

[0061] Again, in the structure of this module (C) , the device temperature sensor (10) , the sensor connection point (11) , and / or the device temperature system connection can be included .

[0062] Figure 7 shows the situation where, after the tube (6) and the cap (5) are connected, it is connected by the cap (5) to an electrochemical analysis module (C) and by the tube to the upper module (D) of the device.

[0063] Within this embodiment, the device upper module (D) is provided with at least one heat transfer element (13) . Within the embodiment of Figure 7, the device upper module (D) is configured as a rectangular body and is positioned in the heat transfer element (13) .

[0064] Figure 8 shows the details of the heat transfer element (13) . Within this embodiment, the heat transfer element (13) is provided with at least one heating element (14) , at least one heat transfer block (15) and / or resistance connection point.

[0065] According to Figure 8, the heat transfer element (13) is configured as a cylinder and is installed around the test tube. Within this embodiment, the heat transfer plate (B) and the heat transfer element (13) are operated in an integrated manner .

[0066] Thus, temperature transfer will be provided from the outer surface of the closed test tube, liquid temperature will be transferred directly out of the cap (5) with the heat conductive plate (B) integrated into the cap (5) , and information will be provided to the heat transfer element (13) for heating by making precise measurement with the temperature probe adjacent to the part of the plate (B) outside the cap (5) . Thus, the temperature on the surface of the biosensor (A) is quickly and precisely controlled and the biosensor (A) measurement differences that occur according to the differences that will occur in external weather conditions will be prevented.

[0067] At the same time, protection will be provided against external contaminations and analyte residues.

Claims

CLAIMS1 . A system providing isolated temperature control for electrochemical sensors (A) suitable for use in field applications , used to perform a precise temperature control reaction in a closed environment , by correlating temperature with reaction kinetics , characterized in that it comprises at least one electrochemical biosensor (A) integrated on the tube cap ( 5 ) of the test tube ( 6 ) and a heat trans fer plate (B ) extending to the electrode level where the reaction occurs .2 . The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that the part of the printed electrochemical biosensor (A) that performs the reaction is configured to remain inside the caps ( 5 ) of the chemical tubes ( 6 ) , while the connection part remains on the outside .

3. The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 or 2 , characterized in that it comprises a heat trans fer plate (B ) arranged parallel to the front or back surface of the electrochemical biosensor (A) .4 . The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that the tube cap ( 5 ) is removably connected to the tube ( 6 ) .5 . The system providing isolated temperature control of electrochemical sensors (A) according to any one of the preceding claims , characterized in that the heat trans fer plate (B ) is configured such that one end extends to thesurface where the electrochemical biosensor (A) performs the reaction, and the other end is designed to pass through the tube cap ( 5 ) and expand on the outer side of the cap ( 5 ) .

6. The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that it comprises an electrochemical biosensor (A) positioned in the centre of the cap ( 5 ) .7 . The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that it comprises an electrochemical biosensor (A) containing at least one working electrode ( 1 ) and at least one reference electrode ( 2 ) in its structure .8 . The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that it comprises an electrochemical biosensor (A) equipped with at least one end integration section ( 4 ) .

9. The system providing isolated temperature control of electrochemical sensors (A) according to claim 7 , characterized in that two reference electrodes ( 2 ) are positioned on both sides of the working electrode ( 1 ) .10 . The system providing isolated temperature control of electrochemical sensors (A) according to claim 1 , characterized in that it comprises the heat trans fer plate (B ) equipped with at least one heat trans fer plate contact point ( 3 ) .11 . The system providing the isolated temperature control of the electrochemical sensors (A) according to claim 10 ,characterized in that it comprises the heat transfer plate contact point (3) , which is wider than the other part and connects the heat transfer plate (B) to the cap (5) .

12. The system that provides isolated temperature control of electrochemical sensors (A) according to claim 1, characterized in that it comprises an electrochemical analysis module (C) that can be connected after the tube (6) and cap (5) are connected.

13. The system providing isolated temperature control of electrochemical sensors (A) according to claim 12, characterized in that it comprises an electrochemical analysis module (C) equipped with a temperature control connection cable (6) , a battery (8) , and / or a system control board ( 9 ) .

14. The system providing isolated temperature control of electrochemical sensors (A) according to claim 12 or 13, characterized in that the device comprises an electrochemical analysis module (C) equipped with a temperature sensor (10) , a sensor connection point (11) , and / or a device temperature system connection.

15. The system providing isolated temperature control of electrochemical sensors (A) according to claim 12, characterized in that it is connected to an electrochemical analysis module (C) by a cap (5) and to the device upper module (D) by a tube.

16. The system providing isolated temperature control of electrochemical sensors (A) according to claim 15, characterized in that it comprises the device upper module (D) equipped with at least one heat transfer element (13) .

17. The system providing isolated temperature control of electrochemical sensors (A) according to claim 15 or 16, characterized in that it comprises the device upper module (D) configured as a rectangular body and positioned within the heat transfer element (13) .

18. The system providing isolated temperature control of electrochemical sensors (A) according to claim 16 or 17, characterized in that the heat transfer element (13) comprises a heat transfer element (13) equipped with at least one heating element (14) , at least one heat transfer block (15) and / or resistance connection point.

19. The system providing isolated temperature control of electrochemical sensors (A) according to claim 16 or 17, characterized in that it comprises a heat transfer element (13) configured in the form of a cylinder and installed around the test tube, in which the heat transfer plate (B) and the heat transfer element (13) are operated integrally.

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