Primer pair, method, and use for rapidly identifying newborn nude mice
By designing imperfectly paired PCR amplification primers, and utilizing PCR-RFLP technology combined with agarose gel electrophoresis and lateral flow method, newborn nude mice can be rapidly identified, solving the problem of difficult identification in existing technologies and achieving low-cost, real-time nude mouse identification.
WO2026081369A1 Publication Date: 2026-04-23CANVEST WUHAN BIOTECH
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Patent Information
- Application Number
- PCT/CN2025/070067
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- Priority Date
- 2024-10-18
- Filing Date
- 2025-01-02
- Publication Date
- 2026-04-23
AI Technical Summary
Technical Problem
Existing technologies make it difficult to quickly and cost-effectively identify newborn nude mice, leading to increased workload and material waste in the safety evaluation of biological products.
Method used
A pair of imperfectly paired PCR amplification primers were designed. Using PCR-RFLP technology, newborn nude mice were identified by agarose gel electrophoresis and lateral flow assay. The primer pair specifically recognizes the Foxn1 gene of nude mice, allowing for direct enzyme digestion without the need for PCR product purification.
Benefits of technology
It enables rapid and low-cost identification of newborn nude mice, simplifies the experimental process, reduces dependence on instruments, and allows for real-time detection.
✦ Generated by Eureka AI based on patent content.
Abstract
A primer pair, method, and use for rapidly identifying newborn nude mice. On the basis that a deletion mutation occurs in the base G at position 337 of a coding region of the Foxn1 gene in nude mice, a primer pair introducing an enzyme digestion site is designed according to sequence characteristics. Using the genome of a mouse to be tested as a template, the Foxn1 gene is amplified according to a PCR-RFLP method. The PCR product is then digested with a SmaI restriction endonuclease, and the digested product is genotyped by agarose gel electrophoresis. In addition, a reaction system that can be combined with lateral flow technology is screened, thereby achieving instant detection of the newborn nude mice. The method has the characteristics of simple operation, short time consumption, low cost, instant detection, etc.
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