Composition for hair growth and method to prepare the same
A composition with specific plant extracts and proteins stimulates hair follicles and promotes growth by upregulating VEGF and enhancing follicular cell proliferation, addressing multifactorial hair loss causes and improving hair health.
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- ANVEYA LIVING PTE LTD
- Filing Date
- 2025-11-10
- Publication Date
- 2026-05-21
Smart Images
Figure IN2025051759_21052026_PF_FP_ABST
Abstract
Description
[0001] COMPOSITION FOR HAIR GROWTH AND METHOD TO PREPARE THE SAME TECHNICAL FIELD
[0002] The present disclosure relates to a composition of hair growth and a method to prepare the same.
[0003] BACKGROUND
[0004] Hair fall is a natural part of the hair growth cycle. However, when the balance between hair growth and shedding is disrupted, it can lead to excessive hair loss, a condition often referred to as hair fall. In recent years, hair loss has become a significant concern, influenced by factors such as lifestyle changes, stress, hormonal imbalances, poor nutrition, medication, and environmental factors.
[0005] There are several types of hair loss patterns, with androgenetic alopecia (AGA) being the most common. Medical conditions, such as hypothyroidism and nutritional deficiencies, as well as the use of certain medications like contraceptives, also contribute to hair loss in both men and women. Recent studies show that by the age of 70, hair loss affects approximately 80% of males and 50% of females. The incidence of hair loss increases with age and varies across different ethnicities, being more common among Caucasians, followed by Asians, and African Americans, and less prevalent in Native Americans and Eskimos.
[0006] Presently, hair fall composition has several drawbacks, including limited effectiveness across all phases of the hair growth cycle and an inability to address underlying causes like lifestyle changes, hormonal imbalances, or nutrient deficiencies. Many rely on synthetic ingredients, which can cause side effects such as scalp irritation or more severe reactions and often provide only temporary results. Additionally, these methods usually focus on single mechanisms like blood flow stimulation or enzyme inhibition, failing to offer a comprehensive approach to the multifactorial nature of hair loss. Age-related hair thinning and follicle miniaturization are often resistant to conventional compositions, which also tend to be less effective across different ethnicities and hair types. Moreover, results can take months to manifest, making the process slow and frustrating, while invasive or expensive procedures like transplants and laser therapy are not viable for everyone.
[0007] Thus, there is a need for a composition for hair growth and a method to prepare the same to overcome the aforementioned drawbacks.
[0008] SUMMARY
[0009] In an aspect of the present disclosure, a composition for hair growth is disclosed. The composition comprising one or more ingredients selected from a group including: Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition, Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition, Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition, Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition, Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition, Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition, biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition, hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition, arginine in a range of 0.05 to 0.2% by volume of the composition, glycine in a range of 0.08 to 0.2% by volume of the composition, caffeine in a range of 0.05 to 0.5% by volume of the composition, panthenol in a range of 0.1 to 1.0% by volume of the composition, niacinamide in a range of 0.5 to 2.0% by volume of the composition, hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, zinc chloride in a range of 0.05 to 0.08% by volume of the composition; and one or more excipients. In some aspects of the present disclosure, the one or more excipients are selected from a group comprising humectants, preservatives, chelating agents, emollients and solubilizers, rheology modifiers, and conditioning agent.
[0010] In some aspects of the present disclosure, the humectants are selected from a group comprising glycerin in a range of 5.0 to 8.0% by volume of the composition, propanediol in a range of 0.5 to 2.0% by volume of the composition.
[0011] In some aspects of the present disclosure, the preservatives are selected from a group comprising sodium metabisulfite in a range of 0.03 to 0.1% by volume of the composition, sodium benzoate in a range of 0.01 to 0.04% by volume of the composition, phenoxyethanol and ethylhexylglycerin in a range of 0.3 to 0.8% by volume of the composition.
[0012] In some aspects of the present disclosure, chelating agents are selected from a group comprising tetrasodium ethylenediaminetetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition, gluconolactone in a range of 0.07 to 0.1% by volume of the composition.
[0013] In some aspects of the present disclosure, emollients and solubilizers are selected from a group comprising polyalkylene glycol mono butyl ether may be in a range of 1.00-4.00% by volume of the composition, PEG-40 hydrogenated castor oil may be in a range of 0.30-0.80% by volume of the composition.
[0014] In some aspects of the present disclosure, rheology modifiers are selected from a group comprising acrylates / C 10-30 alkyl acrylate crosspolymer may be in a range of 0.05-0.30% by volume of the composition.
[0015] In some aspects of the present disclosure, conditioning agents is selected from a group comprising calcium gluconate in a range of 0.05 to 0.08% by volume of the composition.
[0016] In an aspect of the present disclosure, a method of preparing a composition for hair growth is disclosed. The method includes steps- adding demineralized (DM) water to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer in a range of 0.05-0.30 %, Tetrasodium Ethylenediamine Tetra acetic Acid (EDTA) in a range of 0.01-0.1 %, and Niacinamide in a range of 0.50-2.00 %; preparing one or more premixes individually, the one or more premixes comprises demineralized (DM) water, and one or more specific ingredients added to the mixing vessel sequentially; adding ingredients Hamamelis virginiana (Witch Hazel) in a range of 2.00-5.00 %, Larix europea wood extract in a range of 0.08-0.2 %, Glycerin in a range of 5.00-8.00 %, Zinc Chloride in a range of 0.05-0.08 %, Camellia sinesis leaf extract in a range of 0.01-0.05 %, Sodium Metabisulfite in a range of 0.03-0.1 %, Glycine in a range of 0.08-0.2 %, Pisum sativum sprout extract in a range of 0.1 -0.4 %, Curcuma longa callus conditioned media in a range of 5-8 %, Gluconolactone in a range of 0.07-0.1 %, Sodium benzoate in a range of 0.01-0.04 %, Calcium gluconate in a range of 0.05-0.08 %, Saccharide Isomerate in a range of 0.1 -0.5 %, Phenoxyethanol and Ethylhexylglycerin in a range of 0.3-0.8 %, and Polyalkylene Glycol Mono Butyl Ether in a range of 1.00-4.00 %, is added to the mixing vessel. In some aspects of the present disclosure, the one or more premixes comprises: premix A is prepared by dissolving Hydrolyzed Pea Protein in a range of 0.05-0.50 %, Caffeine in a range 0.05-0.50 %, and Panthenol in a range of 0.1-1.00 % in DM water; premix B is prepared by dissolving Hyaluronic acid in a range of 0.01-0.2 %, Biotinoyl Tripeptide- 1 in a range of 0.0001-0.01 %, Alpha Glucosyl Hesperidin in a range of 0.05- 0.50 %, and Hydrolyzed Hyaluronic acid in a range of 0.01-0.2 % in the DM water; premix C is prepared by dissolving Propanediol in a range of 0.5-2.00 %, Peg-40 hydrogenated castor oil in a range of 0.30-0.80 %, and Rosmarinus officinalis Leaf Oil in a range of 0.05-0.20 % in the DM water, and premix D is prepared by dissolving Arginine in a range of 0.05-0.20 % with the DM water.
[0017] In some aspects of the present disclosure, the composition is prepared in a topical form selected from a group comprising serum, lotion, cream, gel, spray, ointment, emulsion, or oil. In an aspect of the present disclosure, a method of treating a subject in need of hair treatment, the method includes topically administring a composition to the scalp of the subject, such that the composition stimulates hair follicles, reduces hair fall, promotes hair growth, and improves hair thickness, density, and strength.
[0018] In some aspects of the present disclosure, the composition is administered in a dosage of 0.5 to 1.0 milliliters per application.
[0019] In some aspects of the present disclosure, the composition is administered to the scalp once or twice daily for a duration of at least 8 weeks to achieve visible improvement in hair density and reduction in hair fall.
[0020] In an aspect of the present disclosure, use of a composition for reducing hair fall, improving hair growth, increasing hair density, enhancing hair thickness, and promoting hair strength.
[0021] In an aspect of the present disclosure, use of a composition including Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition, Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition, Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition, Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition, Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition, Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition, biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition, hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition, arginine in a range of 0.05 to 0.2% by volume of the composition, glycine in a range of 0.08 to 0.2% by volume of the composition, caffeine in a range of 0.05 to 0.5% by volume of the composition, panthenol in a range of 0.1 to 1.0% by volume of the composition, niacinamide in a range of 0.5 to 2.0% by volume of the composition, hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, zinc chloride in a range of 0.05 to 0.08% by volume of the composition, and one or more excipients.
[0022] BRIEF DESCRIPTION OF DRAWINGS
[0023] The above and still further features and advantages of aspects of the present disclosure become apparent upon consideration of the following detailed description of aspects thereof, especially when taken in conjunction with the accompanying drawings, and wherein;
[0024] FIG. 1 illustrates a concentration-dependent increase in VEGF expression, in accordance with an aspect of the present disclosure; and
[0025] FIG. 2 illustrates the enhancement of dermal papilla cell viability, in accordance with an exemplary aspect of the present disclosure.
[0026] To facilitate understanding, reference numerals have been used, where possible, to designate elements common to the figures.
[0027] DETAILED DESCRIPTION OF PREFERRED ASPECTS
[0028] This section is intended to provide an explanation and description of various possible aspects of the present disclosure. The aspects used herein, and the various features and advantageous details thereof are explained more fully with reference to non -limiting aspects illustrated in the accompanying drawing / s and detailed in the following description. The examples used herein are intended only to facilitate an understanding of ways in which the aspects may be practiced and to enable the person skilled in the art to practice the aspects used herein. Also, the examples / aspects described herein should not be construed as limiting the scope of the aspects herein.
[0029] V arious aspects of the present disclosure provide a composition for hair growth and a method to prepare thereof. The following description provides specific details of certain aspects of the disclosure illustrated in the drawings to provide a thorough understanding of those aspects. It should be recognized, however, that the present disclosure can be reflected in additional aspects and the disclosure may be practiced without some of the details in the following description.
[0030] The various aspects including the example aspects are now described more fully with reference to the accompanying drawings, in which the various aspects of the disclosure are shown. The disclosure may, however, be embodied in different forms and should not be construed as limited to the aspects set forth herein. Rather, these aspects are provided so that this disclosure is thorough and complete, and fully conveys the scope of the disclosure to those skilled in the art.
[0031] The subject matter of example aspects, as disclosed herein, is described specifically to meet statutory requirements. However, the description itself is not intended to limit the scope of this disclosure. Rather, the inventor / inventors have contemplated that the claimed subject matter might also be embodied in other ways, to include different features or combinations of features similar to the ones described in this document, in conjunction with other technologies. Generally, the various aspects including the example aspects relate to a composition for hair growth and a method to prepare thereof.
[0032] As mentioned, there is a requirement to develop the composition for hair growth and the method to prepare the same.
[0033] Each and every ingredient of the composition including all the biological materials (including all kind of extracts, oils etc., cell lines and chemicals) used to prepare the claimed composition is commercially procured from India.
[0034] In some aspects, the present disclosure provides the composition for hair growth (hereinafter referred to and designated as “the composition”). The composition may include one or more active ingredients and one or more excipients.
[0035] In some aspects of the present disclosure, the composition may deliver through various formulations. The composition may be formulated into a foam for easy application and even distribution on the scalp, a serum for concentrated use on specific areas, and a spray for quick and convenient application, particularly suited for individuals with long hair.
[0036] In an aspect of the present disclosure, a composition for hair growth is disclosed. The composition comprising one or more ingredients selected from a group including: Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition, Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition, Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition, Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition, Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition, Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition, biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition, hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition, arginine in a range of 0.05 to 0.2% by volume of the composition, glycine in a range of 0.08 to 0.2% by volume of the composition, caffeine in a range of 0.05 to 0.5% by volume of the composition, panthenol in a range of 0.1 to 1.0% by volume of the composition, niacinamide in a range of 0.5 to 2.0% by volume of the composition, hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, zinc chloride in a range of 0.05 to 0.08% by volume of the composition; and one or more excipients.
[0037] In some aspects of the present disclosure, the one or more excipients are selected from a group including humectants, preservatives, chelating agents, emollients and solubilizers, rheology modifiers, and conditioning agent.
[0038] In some aspects of the present disclosure, the humectants are selected from a group including glycerin in a range of 5.0 to 8.0% by volume of the composition, propanediol in a range of 0.5 to 2.0% by volume of the composition. In some aspects of the present disclosure, the preservatives are selected from a group including sodium metabisulfite in a range of 0.03 to 0.1% by volume of the composition, sodium benzoate in a range of 0.01 to 0.04% by volume of the composition, phenoxyethanol and ethylhexylglycerin in a range of 0.3 to 0.8% by volume of the composition.
[0039] In some aspects of the present disclosure, chelating agents are selected from a group including tetrasodium ethylenediaminetetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition, gluconolactone in a range of 0.07 to 0.1% by volume of the composition.
[0040] In some aspects of the present disclosure, emollients and solubilizers are selected from a group including polyalkylene glycol mono butyl ether may be in a range of 1.00-4.00 % by volume of the composition, peg-40 hydrogenated castor oil may be in a range of 0.30-0.80 % by volume of the composition.
[0041] In some aspects of the present disclosure, rheology modifiers are selected from a group including acrylates / C 10-30 alkyl acrylate crosspolymer may be in a range of 0.05-0.30 % by volume of the composition.
[0042] In some aspects of the present disclosure, conditioning agents is selected from a group including calcium gluconate in a range of 0.05 to 0.08% by volume of the composition. In some aspects of the present disclosure, one or more ingredients selected from a group including Larix europea wood extract, Camellia sinensis leaf extract, Pisum sativum sprout extract, Curcuma longa callus conditioned media, Rosmarinus officinalis leaf oil, biotinoyl tripeptide- 1 , caffeine, and zinc chloride may be used as hair growth agents.
[0043] In some aspects of the present disclosure, one or more ingredients selected from a group including hyaluronic acid, panthenol, saccharide isomerate, and ethylhexylglycerin may be used as moisturizing agents. In some aspects of the present disclosure, one or more ingredients selected from a group including glycerin, propanediol, and hydrolyzed hyaluronic acid may be used as humectants.
[0044] In some aspects of the present disclosure, one or more ingredients selected from a group including arginine and glycine may be used as amino acids to support scalp and follicle health.
[0045] In some aspects of the present disclosure, hydrolyzed pea protein may be used as a conditioning agent to improve hair texture and manageability.
[0046] In some aspects of the present disclosure, alpha glucosyl hesperidin may be used as an antioxidant to reduce oxidative stress on scalp tissues.
[0047] In some aspects of the present disclosure, niacinamide may be used as an antiinflammatory agent to soothe the scalp and reduce irritation.
[0048] In some aspects of the present disclosure, Hamamelis virginiana extract may be used as a soothing agent to calm the scalp.
[0049] In some aspects of the present disclosure, water may be added to the composition until a desired volume is achieved. The water may be demineralized (DM) water.
[0050] In some aspects of the present disclosure, Larix europea wood extract in a range 0.08-0.2 % by volume, Camellia sinesis leaf extract in a range 0.01-0.05 % by volume, Pisum sativum sprout extract in a range 0.1 -0.4 % by volume, Curcuma longa (Turmeric) callus conditioned media in a range 5-8 % by volume, Glycerin in a range of 5.00-8.00 % by volume, Glycine in a range of 0.08-0.2% by volume, Zinc Chloride in a range of 0.05-0.08 % by volume, Sodium Metabisulfite in a range of 0.03-0.1 % by volume, Gluconolactone in a range of 0.07-0.1 % by volume, Sodium Benzoate in a range of 0.01-0.04 % by volume, Calcium Gluconate in a range of 0.05-0.08 % by volume, Propanediol in a range of 0.5-2.00 % by volume, Biotinoyl Tripeptide- 1 in a range of 0.0001-0.01 % by volume, and Phenoxyethanol in a range of 0.3 -0.6 % by volume may be added in combination. In some aspects of the present disclosure, a method of preparing the composition may include steps: adding demineralized (DM) water to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer may be in a range of 0.05-0.30 %, Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) may be in a range of 0.01-0.1 %, and Niacinamide may be in a range of 0.50-2.00 %. The ingredients may be thoroughly mixed and homogenized to ensure uniformity and consistency in the base formulation; and preparing one or more separate premixes (A to D) individually, the one or more premixes may include DM water, and one or more specific ingredients added to the mixing vessel sequentially. Premix A may be prepared by dissolving Hydrolyzed Pea Protein may be in a range of 0.05-0.50 %, Caffeine may be in a range 0.05-0.50 %, and Panthenol may be in a range of 0.1-1.00 % in DM water, premix B may be prepared by dissolving Hyaluronic acid may be in a range of 0.01-0.2 %, Biotinoyl Tripeptide-1 may be in a range of 0.0001-0.01 %, Alpha Glucosyl Hesperidin may be in a range of 0.05-0.50 %, and Hydrolyzed Hyaluronic acid may be in a range of 0.01-0.2 % in the DM water, premix C may be prepared by dissolving Propanediol may be in a range of 0.5-2.00 %, Peg-40 hydrogenated castor oil may be in a range of 0.30-0.80 %, and Rosmarinus officinalis Leaf Oil may be in a range of 0.05-0.20 % in the DM water, and premix D may be prepared by dissolving Arginine may be in a range of 0.05-0.20 % with the DM water; and adding remaining ingredients Hamamelis virginiana (Witch Hazel) may be in a range of 2.00-5.00 %, Larix europea wood extract may be in a range of 0.08-0.2 %, Glycerin may be in a range of 5.00-8.00 %, Zinc Chloride may be in a range of 0.05-0.08 %, Camellia sinesis leaf extract may be in a range of 0.01-0.05 %, Sodium Metabisulfite may be in a range of 0.03-0.1 %, Glycine may be in a range of 0.08-0.2 %, Pisum sativum sprout extract may be in a range of 0.1 -0.4 %, Curcuma longa callus conditioned media may be in a range of 5-8 %, Gluconolactone may be in a range of 0.07-0.1 %, Sodium benzoate may be in a range of 0.01-0.04 %, Calcium gluconate may be in a range of 0.05-0.08 %, Saccharide Isomerate may be in a range of 0.1-0.5 %, Phenoxyethanol and Ethylhexylglycerin may be in a range of 0.3-0.8 %, and Polyalkylene Glycol Mono Butyl Ether may be in a range of 1.00-4.00 %, may be added to the mixing vessel. In some aspects of the present disclosure, the composition may be prepared in a topical form selected from a group comprising serum, lotion, cream, gel, spray, ointment, emulsion, or oil. Aspects of the present disclosure are intended to include or otherwise cover any type of forms including known, related art, and / or without deviating from the scope of the present disclosure.
[0051] In an aspect of the present disclosure, a method of treating a subject in need of hair treatment is disclosed. The method includes topically administering the composition to the scalp of the subject, wherein the composition stimulates hair follicles, reduces hair fall, promotes hair growth, and improves hair thickness, density, and strength.
[0052] In some aspects of the present disclosure, the composition is administered in a dosage of 0.5 to 1.0 milliliters per application.
[0053] In some aspects of the present disclosure, the composition is administered to the scalp once or twice daily for a duration of at least 8 weeks to achieve visible improvement in hair density and reduction in hair fall.
[0054] In some aspects of the present disclosure, use of the composition may include for reducing hair fall, improving hair growth, increasing hair density, enhancing hair thickness, and promoting hair strength.
[0055] In some aspect of the present disclosure, use of a composition including Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition, Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition, Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition, Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition, Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition, Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition, biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition, hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition, hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition, arginine in a range of 0.05 to 0.2% by volume of the composition, glycine in a range of 0.08 to 0.2% by volume of the composition, caffeine in a range of 0.05 to 0.5% by volume of the composition, panthenol in a range of 0.1 to 1.0% by volume of the composition, niacinamide in a range of 0.5 to 2.0% by volume of the composition, hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition, zinc chloride in a range of 0.05 to 0.08% by volume of the composition, and one or more excipients.
[0056] Examples:
[0057] The disclosure will now be illustrated with working examples, which are intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed composition. It is to be understood that this disclosure is not limited to methods, and experimental conditions described, as such methods and conditions may vary.
[0058] EXAMPLE 1
[0059] In exemplary aspects of the present disclosure, the composition includes, Larix europea wood extract in 0.12 % by volume of the composition, Camellia sinesis leaf extract in 0.025 % by volume of the composition, Pisum sativum sprout extract in 0.18 % by volume of the composition, Curcuma longa (Turmeric) callus conditioned media in 6 % by volume of the composition, Hamamelis virginiana (Witch Hazel) in 3.2 % by volume of the composition, Rosmarinus officinalis Leaf Oil in 0.1 % by volume of the composition, Glycerin in 5.5 % by volume of the composition, Glycine in 0.11 % by volume of the composition, Zinc Chloride in 0.07 % by volume of the composition, Sodium Metabisulfite in 0.05 % by volume of the composition, Gluconolactone in 0.085 % by volume of the composition, Sodium Benzoate in 0.018 % by volume of the composition, Calcium Gluconate in 0.065 % by volume of the composition, Tetrasodium ethylenediaminetetraacetic acid (EDTA) in 0.025 % by volume of the composition, Saccharide Isomerate in 0.18 % by volume of the composition, Hyaluronic Acid in 0.13 % by volume of the composition, Hydrolyzed Hyaluronic Acid in 0.15 % by volume of the composition, Propanediol in 1 % by volume of the composition, Biotinoyl Tripeptide- 1 in 0.0015 % by volume of the composition, Alpha Glucosyl Hesperidin in 0.13 % by volume of the composition, Caffeine in 0.18 % by volume of the composition, Hydrolyzed Pea Protein in 0.1 % by volume of the composition, Polyalkylene Glycol Mono Butyl Ether in 2.5 % by volume of the composition, Acrylates / C 10-30 Alkyl Acrylate Crosspolymer in 0.12 % by volume of the composition, Panthenol in 0.65 % by volume of the composition, Niacinamide in 1.1 % by volume of the composition, Arginine in 0.11 % by volume of the composition, PEG-40 Hydrogenated Castor Oil in 0.58 % by volume of the composition, Ethylhexylglycerin in 0.65 % by volume of the composition, Phenoxyethanol in 0.58 % by volume of the composition and water is added to the composition until the desired volume is achieved.
[0060] EXAMPLE 2
[0061] Demineralized (DM) water was added to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer (0.12%), Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) (0.025%), and Niacinamide (1.1%). The ingredients are thoroughly mixed and homogenized to ensure uniformity and consistency in the base formulation; b. preparing one or more separate premixes (A to D) individually, the one or more premixes may include DM water, and one or more specific ingredients added to the mixing vessel sequentially. Premix A is prepared by dissolving Hydrolyzed Pea Protein in (0.1%), Caffeine (0.18%), and Panthenol (0.65 %) in DM water, premix B is prepared by dissolving Hyaluronic acid (0.13%), Biotinoyl Tripeptide-1 (0.0015%), Alpha Glucosyl Hesperidin (0.13%), and Hydrolyzed Hyaluronic acid (0.15%) in the DM water, premix C is prepared by dissolving Propanediol (1%), PEG-40 hydrogenated castor oil (0.58%), and Rosmarinus officinalis Leaf Oil (0.1%) in the DM water, and premix D is prepared by dissolving Arginine (0.11%) with the DM water; and c. adding remaining ingredients Hamamelis virginiana (Witch Hazel) (3.2%), Larix europea wood extract (0.12%), Glycerin (5.5%), Zinc Chloride (0.07%), Camellia sinesis leaf extract (0.025%), Sodium Metabisulfite (0.05%), Glycine (0.11%), Pisum sativum sprout extract (0.18 %), Curcuma longa callus conditioned media (6 %), Gluconolactone (0.085%), Sodium benzoate (0.018%), Calcium gluconate (0.065%), Saccharide Isomerate (0.18%), Phenoxyethanol (0.58%) and Ethylhexylglycerin (0.65%), and Polyalkylene Glycol Mono Butyl Ether (2.5 %), is added to obtain the composition.
[0062] EXAMPLE 3
[0063] Demineralized (DM) water was added to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer (0.27%), Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) (0.085%), and Niacinamide (1.8%). The ingredients are thoroughly mixed and homogenized to ensure uniformity and consistency in the base formulation; b. preparing one or more separate premixes (A to D) individually, the one or more premixes may include DM water, and one or more specific ingredients added to the mixing vessel sequentially. Premix A is prepared by dissolving Hydrolyzed Pea Protein in (0.45%), Caffeine (0.4%), and Panthenol (0.95 %) in DM water, premix B is prepared by dissolving Hyaluronic acid (0.195%), Biotinoyl Tripeptide-1 (0.01%), Alpha Glucosyl Hesperidin (0.48%), and Hydrolyzed Hyaluronic acid (0.20%) in the DM water, premix C is prepared by dissolving Propanediol (1.80%), PEG-40 hydrogenated castor oil (0.78%), and Rosmarinus officinalis Leaf Oil (0.18%) in the DM water, and premix D is prepared by dissolving Arginine (0.20%) with the DM water; and c. adding remaining ingredients Hamamelis virginiana (Witch Hazel) (5%), Larix europea wood extract (0.18%), Glycerin (7.15%), Zinc Chloride (0.08%), Camellia sinesis leaf extract (0.045%), Sodium Metabisulfite (0.95%), Glycine (0.18%), Pisum sativum sprout extract (0.40%), Curcuma longa callus conditioned media (7.5%), Gluconolactone (0.10%), Sodium benzoate (0.035%), Calcium gluconate (0.08%), Saccharide Isomerate (0.40%), Phenoxyethanol (0.80%) and Ethylhexylglycerin (0.75%), and Polyalkylene Glycol Mono Butyl Ether (3.80%), is added to obtain the composition.
[0064] EXAMPLE 4
[0065] Demineralized (DM) water was added to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer (0.06%), Tetrasodium Ethylenediamine Tetraacetic Acid (EDTA) (0.012%), and Niacinamide (0.55%). The ingredients are thoroughly mixed and homogenized to ensure uniformity and consistency in the base formulation; b. preparing one or more separate premixes (A to D) individually, the one or more premixes may include DM water, and one or more specific ingredients added to the mixing vessel sequentially. Premix A is prepared by dissolving Hydrolyzed Pea Protein in (0.055%), Caffeine (0.058%), and Panthenol (0.20%) in DM water, premix B is prepared by dissolving Hyaluronic acid (0.06%), Biotinoyl Tripeptide-1 (0.0001%), Alpha Glucosyl Hesperidin (0.055%), and Hydrolyzed Hyaluronic acid (0.01%) in the DM water, premix C is prepared by dissolving Propanediol (0.55%), PEG-40 hydrogenated castor oil (0.35%), and Rosmarinus officinalis Leaf Oil (0.055%) in the DM water, and premix D is prepared by dissolving Arginine (0.06%) with the DM water; and c. adding remaining ingredients Hamamelis virginiana (Witch Hazel) (2%), Larix europea wood extract (0.08%), Glycerin (5.1%), Zinc Chloride (0.055%), Camellia sinesis leaf extract (0.012%), Sodium Metabisulfite (0.033%), Glycine (0.08%), Pisum sativum sprout extract (0.12%), Curcuma longa callus conditioned media (5%), Gluconolactone (0.07%), Sodium benzoate (0.012%), Calcium gluconate (0.05%), Saccharide Isomerate (0.05%), Phenoxyethanol (0.35%) and Ethylhexylglycerin (0.30%), and Polyalkylene Glycol Mono Butyl Ether (3%), is added to obtain the composition. Overall working:
[0066] { Control- components without claimed composition
[0067] Treated- with components with claimed composition }
[0068] The study collectively demonstrated that the present composition exerts a dual mechanism of action relevant to hair growth and follicular health. In the VEGF expression study (study 1), administration of the composition significantly upregulated Vascular Endothelial Growth Factor, a critical biomarker for angiogenesis and follicular nourishment. Within 48 hours, dermal papilla cells exhibited up to a 33 percent (Refer to Fig. 1) increase in VEGF expression in a clear dose -dependent manner. This rapid upregulation of VEGF pinpoints the molecular driver of efficacy, as this enhances follicular perfusion and nutrient delivery, thereby supporting the favorable progression of follicles into the growth phase, which underlies visible hair regeneration.
[0069] In parallel, the dermal papilla cell viability study established the direct impact of the composition on follicular cell survival and proliferation. Administration of the composition resulted in a concentration-dependent increase in cell viability, reaching approximately 1.6-fold (159.74%) higher levels compared to controls. Vehicle controls showed minimal effect (98.33% viability), confirming that the observed increase was uniquely attributable to the active composition. This early biomarker response at the cellular level provides predictive evidence of potential human clinical outcomes. The dose-dependent increase also establishes a robust and predictable pharmacological response, highlighting efficacy not captured by prior clinical trials that measured only macroscopic hair parameters.
[0070] Together, these findings confirm that the composition promotes hair growth through two complementary pathways: • stimulation of angiogenesis via VEGF upregulation; and
[0071] • reinforcement of follicular cell proliferation and survival.
[0072] The presence of a dose response relationship, reproducible cellular outcomes, and early activation within 48 hours provides robust mechanistic evidence, establishing both the biological pathway and the cellular basis that translate into clinical outcomes of improved hair growth and regeneration.
[0073] The above Clinical trial studies in detail:
[0074] Study 1: The study was conducted with the objective of evaluating the effect of the composition on the expression of Vascular Endothelial Growth Factor (VEGF) in dermal papilla cells derived from human hair follicle dermal stem cells. VEGF is a key signaling protein responsible for angiogenesis and has been implicated in promoting and sustaining hair growth by enhancing blood supply to hair follicles. The experiment aimed to determine whether the composition stimulates VEGF expression, thereby supporting the potential efficacy in hair growth applications.
[0075] Materials and Methods
[0076] Dermal papilla cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 2 millimolar L-glutamine, 10 % fetal bovine serum (FBS), and an antibiotic-antimycotic solution comprising 1000 milligrams per milliliter streptomycin sulfate, 1000 units per milliliter penicillin G sodium, and 2.5 milligrams per milliliter amphotericin B. The cultured cells were maintained under controlled laboratory conditions until they reached 70 to 80 % confluence. Dermal papilla cells were procured from Life Technologies India Pvt. Ltd.
[0077] Two types of controls were maintained: a negative control consisting of the same cell line in complete DMEM medium without composition, and a blank control containing only the medium.
[0078] Procedure Once the cells reached the desired confluence, they were administered with the composition at concentrations of 0.63%, 1.25%, 2.50%, and 5.00%. The cells were incubated for 48 hours under standard conditions. Following incubation, secretome analysis was performed to examine changes in the secretion profile of the cells.
[0079] Thereafter, the cells were subjected to trypsin process three to four times, followed by centrifugation to prepare the cell lysate. The supernatant was removed, and one milliliter of phosphate -buffered saline (PBS) was added to the pellet to wash and stabilize the lysate. DNA was extracted from the cell lysate using a magnetic beadbased DNA isolation method. The purity of the isolated DNA was assessed spectrophotometrically, and the absorbance ratio at 260:280 nanometers was found to be approximately 1.8, indicating satisfactory purity for downstream applications. Expression analysis of VEGF was carried out using reverse transcriptase polymerase chain reaction (RT-PCR) with primers specific to the VEGF gene. The RT-PCR thermal cycling was conducted according to the parameters described below (Table 1):
[0080]
[0081] Table 1: RT-PCR Thermocycling Parameters Results
[0082] The experiment measured the fold-change in VEGF expression across different concentrations of the composition, compared to the control group. The data showed a progressive, concentration-dependent increase in VEGF expression. The following table summarizes the mean expression levels and corresponding standard deviations (Table 2):
[0083]
[0084] Table 2: VEGF Expression Analysis
[0085] The graphical representation (as shown in FIG. 1) illustrates the fold-change in VEGF gene expression in dermal papilla cells following administration with varying concentrations of the composition. The composition at concentrations of 0.63%, 1.25%, 2.5%, and 5.0% resulted in progressively higher expression levels of 11.8%, 17.6%, 23.8%, and 33.1%, respectively.
[0086] Observations and Conclusion
[0087] It was observed that the composition resulted in a statistically significant and concentration-dependent upregulation of VEGF expression in dermal papilla cells. In comparison to the control group, which recorded a mean VEGF expression of 139.00, the expression increased progressively with rising concentrations of the composition. The maximum expression level was recorded at 5.00 % concentration, where a 33.1 % increase in VEGF expression within 48 hours was observed relative to the control. These findings highlight VEGF as a mechanistic biomarker for hair regrowth. Within just 48 hours, the composition upregulated VEGF in dermal papilla cells by up to 33%, demonstrating a clear dose-dependent effect. This rapid upregulation pinpoints the molecular driver that supports follicular activation and improved nutrient delivery, ultimately enhancing the anagen phase relative to the telogen phase. The results underscore that VEGF stimulation represents the earliest measurable biological activity preceding visible hair growth, providing mechanistic support for the composition's efficacy in hair regrowth applications.
[0088] Study 2: The study was conducted with the objective of evaluating the effect of the composition on the viability and proliferation of dermal papilla cells (DPCs) derived from human hair follicle dermal stem cells, using the MTT assay. Dermal papilla cells play a vital role in hair follicle formation, growth regulation, and cycling.
[0089] Materials and Methods:
[0090] Dermal papilla cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 2 millimolar L-glutamine, 10 % fetal bovine serum (FBS), and an antibiotic-antimycotic solution consisting of 1000 milligrams per milliliter streptomycin sulfate, 1000 units per milliliter penicillin G sodium, and 2.5 milligrams per milliliter amphotericin B. Cultures were maintained under standard laboratory conditions at 37°C with 5 % CO2 until they reached 70 to 80 % confluence.
[0091] The composition was diluted in IX phosphate -buffered saline (PBS) to prepare test concentrations of 0.625 %, 1.25 %, 2.5 %, and 5.0 %, in accordance with OECD Guideline 129. Each solution was sterilized using a 0.22-micron syringe filter to avoid microbial contamination.
[0092] Additional materials used included:
[0093] • Cell culture media (Himedia): Dulbecco’s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin (Pen- Strep)
[0094] • Other cell culture reagents: Phosphate buffered saline (PBS), Trypsin-EDTA solution.
[0095] • MTT (Himedia) stock solution prepared at 5 mg / ml in PBS • DMSO (Himedia) for solubilization of formazan crystals
[0096] • 96-well plates (Tarsons) for seeding and processing
[0097] • Multimode Reader (Agilent BioTek Synergy Hl) for measuring absorbance at 570 nm
[0098] Two types of controls were maintained during the study: a control, where dermal papilla cells were cultured in complete DMEM medium without exposure to the composition, and a vehicle control, in which the cells were applied with phosphate -buffered saline (PBS) alone.
[0099] Procedure:
[0100] The composition was prepared in accordance with OECD Guideline 129 and was sterilized using a 0.22-micron syringe filter to ensure the prevention of microbial contamination.
[0101] For the assay, dermal papilla cells were cultured in 25 cm3flasks using Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin-Streptomycin (Pen-Strep). Following culturing, the cells were counted using a hemocytometer, and the total cell concentration was calculated using the formula:
[0102] Total cells / ml = (Total cells counted x Dilution factor x 10,000 cells / ml) / Number of squares counted
[0103] = (204 x 10 x 104cells / ml) / 4
[0104] = 5.1 x 106cells / ml
[0105] The cells were then seeded into 96-well plates at a density of 10,000 cells per well. After seeding, the plates were incubated for 24 hours at 37°C in a humidified atmosphere containing 5% CO2. Following this incubation, cells were administered with the test composition at concentrations of 0.625%, 1.25%, 2.5%, and 5.0%, and then incubated for an additional 24 hours under the same conditions. Post incubation, the media in each well was replaced with 50 pl of MTT-containing media (prepared using 5 mg / ml MTT in PBS), and the plates were incubated for 3 hours at 37°C to allow for the formation of formazan crystals. Subsequently, the MTT-containing media was removed, and 100 pl of DMSO was added to each well to dissolve the crystals. The plates were wrapped in aluminum foil and incubated at room temperature for 30 minutes. Finally, the absorbance was measured at 570 nm using a multimode plate reader (Agilent BioTek Synergy Hl). The cell viability was calculated by comparing the optical density (OD) values of samples to that of the control.
[0106] Formula for Cell Viability Calculation
[0107] The % Cell viability was calculated by using the formula
[0108] % Cell Viability = (Average of OD treated cells / A verage of OD untreated cells) * 100 Results:
[0109] The claimed composition demonstrated a concentration-dependent increase in dermal papilla cell viability, indicating the potential to promote cellular proliferation relevant to hair growth applications.
[0110]
[0111]
[0112] Table 3: Absorbance Measurements at 570 nm
[0113]
[0114] The graphical representation (as shown in FIG. 2) illustrates the concentration -dependent effect of the composition on dermal papilla cell viability. The bar graph shows an upward trend in cell viability as the concentration of the composition increases from 0.625% to 5.00%. The control is set at 100%, and the vehicle control remains comparable at 98.33%, indicating no significant effect from the solvent alone. In contrast, the composition led to a marked increase in viability, reaching a peak of 159.74% at the 5.00% concentration.
[0115] Mechanism of Action for hair serum:
[0116] VEGF Report (Hair Growth Mechanism)
[0117] • VEGF is a natural protein that helps grow new blood vessels and supports the active growth phase of hair.
[0118] • Within just 48 hours, the hair serum increased VEGF levels in hair follicle cells by up to 33%, depending on how much was used. This means the product helps trigger the body’s natural processes that promote hair growth. • This increase in VEGF is a key early sign (biomarker) showing that the serum is working at the biological level.
[0119] • More VEGF — better blood flow and nutrient delivery to hair roots — longer and healthier growth phase (anagen).
[0120] • The serum works quickly, stimulating VEGF production in the cells that control hair growth, which eventually leads to visible hair regrowth.
[0121] • This VEGF increase happens before you can see any visible results, making it a strong indicator of the product’s effectiveness from the start.
[0122] Cell Viability (Follicle Health)
[0123] • Cell viability shows how healthy and alive the hair follicle cells are a better and earlier sign of effectiveness than just counting hairs.
[0124] • The serum increased the survival of hair follicle cells by about 1.6 times (159.74%), which is a strong result at the cellular level even before clinical results are visible.
[0125] • In contrast, a placebo (vehicle control) showed no such improvement (only 98.33%), confirming that the boost is due to the active serum.
[0126] • The serum helps keep hair follicle cells alive and healthy, which is crucial for long-term hair regrowth.
[0127] • The response was dose-dependent meaning more product led to better results showing a clear and consistent biological effect that earlier studies did not fully capture.
[0128] Observations and Conclusion:
[0129] It was observed that the composition resulted in a clear, concentration -dependent increase in dermal papilla cell viability. Cells exposed to the composition showed progressively higher absorbance values at 570 nm compared to the control, indicating enhanced cellular metabolic activity and proliferation. According to the data presented in Table 3, the average absorbance for the control was 0.821, while the composition at 5.0% concentration recorded the highest average absorbance of 1.311. This trend was consistent across all concentrations tested, reflecting increasing cellular viability with increasing doses.
[0130] The corresponding percent viability values, detailed in Table 4, showed that the composition increased cell viability by approximately 114.7% at the lowest concentration (0.625%) and up to 159.7% at the highest concentration (5.0%) when compared to the untreated group. The vehicle control (PBS) exhibited minimal impact, with viability values closely matching the control, thereby confirming that the observed effect was attributable to the composition itself.
[0131] These findings demonstrate that dermal papilla cell viability serves as an early and sensitive biomarker for follicular health, providing insight beyond macroscopic clinical observations. The composition led to a concentration-dependent increase in cell viability, achieving up to a 1.6-fold enhancement at 5.0% concentration, while vehicle controls showed no significant effect. This robust response establishes the composition’s efficacy at the cellular level, indicating predictable pharmacological behavior and suggesting potential to drive improved hair growth outcomes prior to observable clinical effects. The findings highlight that enhanced dermal papilla cell survival is uniquely attributable to the active composition, reinforcing its mechanistic relevance in promoting follicular regeneration.
[0132] Certain advantages of the composition of the present disclosure are listed hereinbelow:
[0133] • The composition stimulates hair follicle stem cells and dermal papilla cells, promoting the active anagen (growth) phase of the hair cycle. Thus, results in increased hair growth, density, hair fall reduction, and thickness, making it particularly useful for individuals experiencing hair thinning or loss.
[0134] • The composition helps to prevent hair follicle shrinking, which is a typical problem in androgenetic alopecia. The composition maintains that hair stays thick and keeps growing normally by preserving normal follicle size, which promotes long-term hair health.
[0135] • The inclusion of natural compounds in the composition provides a sustainable and biocompatible solution for treating hair fall.
[0136] • The composition reduces scalp issues such as itchiness, dryness, and redness, enhancing overall scalp health.
[0137] • The combination of Rosemary’s phenolic compounds, such as rosmarinic acid, provides a shield against oxidative stress.
[0138] • The ingredients such as Rosemary Oil have been shown to stimulate hair follicles and promote hair growth.
[0139] • The ingredients such as Panthenol, and Hyaluronic acid provide hydration and nourishment to the hair and scalp.
[0140] • Antioxidants from Camellia Sinensis Leaf Extract help protect hair from oxidative stress and environmental damage.
[0141] The foregoing discussion of the present disclosure has been presented for purposes of illustration and description. It is not intended to limit the present disclosure to the form or forms disclosed herein. In the foregoing Detailed Description, for example, various features of the present disclosure are grouped together in one or more aspects, configurations, or aspects for the purpose of streamlining the disclosure. The features of the aspects, configurations, or aspects may be combined in alternate aspects, configurations, or aspects other than those discussed above. This method of disclosure is not to be interpreted as reflecting an intention the present disclosure requires more features than are expressly recited in each claim. Rather, as the following claims reflect, inventive aspects lie in less than all features of a single foregoing disclosed aspect, configuration, or aspect. Thus, the following claims are hereby incorporated into this Detailed Description, with each claim standing on its own as a separate aspect of the present disclosure. Moreover, though the description of the present disclosure has included a description of one or more aspects, configurations, or aspects and certain variations and modifications, other variations, combinations, and modifications are within the scope of the present disclosure, e.g., as may be within the skill and knowledge of those in the art, after understanding the present disclosure. It is intended to obtain rights that include alternative aspects, configurations, or aspects to the extent permitted, including alternate, interchangeable, and / or equivalent structures, functions, a range or steps to those claimed, whether or not such alternate, interchangeable and / or equivalent structures, functions, ranges or steps are disclosed herein, and without intending to publicly dedicate any patentable subject matter.
Claims
We Claim1. A composition for hair growth, the composition comprising one or more ingredients selected from a group comprising:• Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition,• Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition,• Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition,• Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition,• Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition,• Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition,• biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition,• hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition, • alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition,• hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition,• arginine in a range of 0.05 to 0.2% by volume of the composition,• glycine in a range of 0.08 to 0.2% by volume of the composition,• caffeine in a range of 0.05 to 0.5% by volume of the composition,• panthenol in a range of 0.1 to 1.0% by volume of the composition,• niacinamide in a range of 0.5 to 2.0% by volume of the composition,• hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition,• saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition,• zinc chloride in a range of 0.05 to 0.08% by volume of the composition; and • one or more excipients.
2. The composition as claimed in claim 1, wherein the one or more excipients are selected from a group comprising- humectants, preservatives, chelating agents, emollients and solubilizers, rheology modifiers, and conditioning agent.
3. The composition as claimed in claim 2, wherein the humectants are selected from a group comprising- Glycerin in a range of 5.0 to 8.0% by volume of the composition, Propanediol in a range of 0.5 to 2.0% by volume of the composition.
4. The composition as claimed in claim 2, wherein the preservatives are selected from a group comprising-Sodium metabisulfite in a range of 0.03 to 0.1% by volume of the composition, Sodium benzoate in a range of 0.01 to 0.04% by volume of the composition, Phenoxyethanol and Ethylhexylglycerin in a range of 0.3 to 0.8% by volume of the composition.
5. The composition as claimed in claim 2, wherein chelating agents are selected from a group comprising- Tetrasodium ethylenediaminetetraacetic acid (EDTA) in a range of 0.01 to 0.1% by volume of the composition, and Gluconolactone in a range of 0.07 to 0.1% by volume of the composition.
6. The composition as claimed in claim 2, wherein emollients and solubilizers are selected from a group comprising- Polyalkylene Glycol Mono Butyl Ether may be in a range of 1.00-4.00 % by volume of the composition, and PEG-40 Hydrogenated Castor Oil may be in a range of 0.30-0.80 % by volume of the composition.
7. The composition as claimed in claim 2, wherein rheology modifiers is selected from a group comprising- Acrylates / C 10-30 Alkyl Acrylate Crosspolymer may be in a range of 0.05-0.30 % by volume of the composition.
8. The composition as claimed in claim 2, wherein conditioning agents is selected from a group comprising- Calcium gluconate in a range of 0.05 to 0.08% by volume of the composition.
9. A method of preparing a composition for hair growth, the method comprising: a. adding demineralized (DM) water to a mixing vessel, followed by the gradual addition of Acrylates / C 10-30 Alkyl Acrylate Crosspolymer in a range of 0.05-0.30 %, Tetrasodium Ethylenediamine Tetra acetic Acid (EDTA) in a range of 0.01-0.1 %, and Niacinamide in a range of 0.50- 2.00 %;b. preparing one or more premixes individually, the one or more premixes comprises demineralized (DM) water, and one or more specific ingredients added to the mixing vessel sequentially; andc. adding ingredients Hamamelis virginiana (Witch Hazel) in a range of 2.00- 5.00 %, Larix europea wood extract in a range of 0.08-0.2 %, Glycerin in a range of 5.00-8.00 %, Zinc Chloride in a range of 0.05-0.08 %, Camellia sinesis leaf extract in a range of 0.01-0.05 %, Sodium Metabisulfite in a range of 0.03-0.1 %, Glycine in a range of 0.08-0.2 %, Pisum sativum sprout extract in a range of 0.1 -0.4 %, Curcuma longa callus conditioned media in a range of 5-8 %, Gluconolactone in a range of 0.07-0.1 %, Sodium benzoate in a range of 0.01-0.04 %, Calcium gluconate in a range of 0.05- 0.08 %, Saccharide Isomerate in a range of 0.1-0.5 %, Phenoxyethanol and Ethylhexylglycerin in a range of 0.3-0.8 %, and Polyalkylene Glycol Mono Butyl Ether in a range of 1.00-4.00 %, to obtain the composition.
10. The method as claimed in claim 9, wherein the one or more premixes comprises:a. premix A is prepared by dissolving Hydrolyzed Pea Protein in a range of 0.05-0.50 %, Caffeine in a range 0.05-0.50 %, and Panthenol in a range of 0.1-1.00 % in DM water;b. premix B is prepared by dissolving Hyaluronic acid in a range of 0.01 - 0.2 %, Biotinoyl Tripeptide- 1 in a range of 0.0001-0.01 %, Alpha Glucosyl Hesperidin in a range of 0.05- 0.50 %, and Hydrolyzed Hyaluronic acid in a range of 0.01-0.2 % in the DM water;c. premix C is prepared by dissolving Propanediol in a range of 0.5- 2.00 %, Peg-40 hydrogenated castor oil in a range of 0.30-0.80 %, and Rosmarinus officinalis Leaf Oil in a range of 0.05-0.20 % in the DM water; andd. premix D is prepared by dissolving Arginine in a range of 0.05-0.20 % with the DM water.
11. The method as claimed in claim 9, wherein the composition is prepared in a topical form selected from a group comprising: serum, lotion, cream, gel, spray, ointment, emulsion, or oil.
12. A method of treating a subject in need of hair treatment, the method comprising topically administering a composition to the scalp of the subject, wherein the composition stimulates hair follicles, reduces hair fall, promotes hair growth, and improves hair thickness, density, and strength.
13. The method as claimed in claim 12, wherein the composition is administered in a dosage of 0.5 to 1.0 milliliters per application.
14. The method as claimed in claim 12, wherein the composition is administered to the scalp once or twice daily for a duration of at least 8 weeks to achieve visible improvement in hair density and reduction in hair fall.
15. Use of a composition for reducing hair fall, improving hair growth, increasing hair density, enhancing hair thickness, and promoting hair strength.
16. The use as claimed in claim 15, wherein the composition comprises:• Larix europea wood extract in a range of 0.08 to 0.2% by volume of the composition,• Camellia sinensis leaf extract in a range of 0.01 to 0.05% by volume of the composition,• Pisum sativum sprout extract in a range of 0.1 to 0.4% by volume of the composition,• Curcuma longa callus conditioned media in a range of 5 to 8% by volume of the composition,• Hamamelis virginiana extract in a range of 2.0 to 5.0% by volume of the composition,• Rosmarinus officinalis leaf oil in a range of 0.05 to 0.20% by volume of the composition,• biotinoyl tripeptide- 1 in a range of 0.0001 to 0.01% by volume of the composition,• hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition,• alpha glucosyl hesperidin in a range of 0.05 to 0.5% by volume of the composition,• hydrolyzed pea protein in a range of 0.05 to 0.5% by volume of the composition,• arginine in a range of 0.05 to 0.2% by volume of the composition, • glycine in a range of 0.08 to 0.2% by volume of the composition, • caffeine in a range of 0.05 to 0.5% by volume of the composition, • panthenol in a range of 0.1 to 1.0% by volume of the composition, • niacinamide in a range of 0.5 to 2.0% by volume of the composition,• hydrolyzed hyaluronic acid in a range of 0.01 to 0.2% by volume of the composition,• saccharide isomerate in a range of 0.1 to 0.5% by volume of the composition,• zinc chloride in a range of 0.05 to 0.08% by volume of the composition, and• one or more excipients.