Grna probe library and preparation and design methods therefor, methods for targeting target sequence and nucleic acid imaging, and use thereof
By designing and preparing gRNA probe libraries, the challenge of imaging low-repetition or non-repetition genomic regions using CRISPR systems has been solved, enabling low-cost, high-efficiency multi-site imaging and gene editing, which is suitable for multi-omics analysis and disease diagnosis in live cells.
WO2026130589A2PCT designated stage Publication Date: 2026-06-25TSINGHUA UNIVERSITY
Patent Information
- Authority / Receiving Office
- WO · WO
- Patent Type
- Applications
- Current Assignee / Owner
- TSINGHUA UNIVERSITY
- Filing Date
- 2026-02-14
- Publication Date
- 2026-06-25
Smart Images
Figure PCTCN2026079468-FTAPPB-I100001 
Figure PCTCN2026079468-FTAPPB-I100002 
Figure PCTCN2026079468-FTAPPB-I100003
Abstract
Provided in the present application are a gRNA probe library and a preparation method and design method therefor, and a method for targeting a target gene and a nucleic acid imaging method. In the present application, a gRNA probe containing a modifying group is bound to a nuclease, which binds to a target genomic sequence or RNA transcript in a living cell, thereby realizing dynamic observation of DNA and RNA. By using fluorescently labeled crRNAs, tracrRNAs and sgRNAs, the present application realizes real-time imaging of repetitive and non-repetitive loci and establishes a robust gene imaging toolkit. In addition, the gRNA probe library prepared in the present application will be applied to a CRISPR system for gene editing, transcriptional regulation, and gene targeting and delivery.
Need to check novelty before this filing date? Find Prior Art