System and method for cold plasma tissue treatment

Abstract

A tissue treatment system (100) for providing a cold plasma treatment. A pulser (102) is configured to deliver in use a set of high-voltage pulses (Pp) to a plasma pad (101) for generating cold plasma of a pre-determined intensity. The pulser comprises a pulse modulation circuit (20) for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses (Pp). A geospatial unit (103) is configured to determine geospatial information (M) of the pulser (102). A feedforward controller (104) is configured to perform a initialization of the pulser (102) prior to delivering the set of high-voltage pulses (Pp). The initialization comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses (Pp) based on a pre-determined relationship between the geospatial information (M) and the pre-determined intensity for generation of cold plasma that is substantially the same as at the sea level.

Description

[0001] Title: SYSTEM AND METHOD FOR COLD PLASMA

[0002] TISSUE TREATMENT

[0003] TECHNICAL FIELD AND BACKGROUND

[0004] The present disclosure relates to a system for cold plasma tissue treatment and a method thereof.

[0005] Cold plasma treatment has emerged as a promising therapy for various medical conditions, particularly due to its antimicrobial, wound healing, and tissue regeneration properties. The general principle of plasma treatment involves generating plasma using Dielectric Barrier Discharge (DBD) technology. This is achieved by applying repetitive, short high-voltage pulses to a planar electrode arrangement. A high voltage source delivers pulsed voltages ranging from 3 to 8 kilovolts, with pulse durations between 1 nanosecond and 150 microseconds, and repetition rates from 0.5 to 100 kilohertz. These parameters allow for micro-discharges during each pulse, enabling electrical current to flow through the object being treated -such as human skin - only when the plasma is active. Between pulses, the plasma becomes inactive, and no current flows through the skin, enhancing safety and control over the treatment process. The electrode arrangement used for the plasma treatment comprises a flexible plasma pad placed above the wound and a ground electrode placed elsewhere on the human skin such that electric current can flow from the plasma pad to the ground electrode.

[0006] Despite its versatility, there are significant challenges related to delivering cold plasma treatments. A significant issue with a plasma treatment device is an electromagnetic interference caused by high voltage oscillations and irregular discharges of the cold plasma. The open mesh design of the flexible plasma pad can act like radio antennas, emitting electromagnetic radiation and causing interference. Another issue is providing a consistent plasma treatment across different clinical sites due to the sensitivity of the plasma generation to various conditions, leading to inconsistent treatment outcomes. For example, changes in temperature may result into underpowered plasma in cold environments or overly aggressiveplasma in hot climates. Humidity brings water vapor into the air, which can significantly alter the plasma's composition. Consequently, the same plasma generation device used at one site may produce a less consistent plasma at another site, potentially causing tissue damage.

[0007] Given the challenges of cold plasma treatment methods, there is a need for a novel solution capable of delivering consistent cold plasma treatments.

[0008] SUMMARY

[0009] The inventors have realized that the variability of cold plasma treatment at different geographical locations can be improved by initializing pulser of a cold plasma treatment system based on geospatial information such as a geographical location, an altitude, and / or an atmospheric pressure.

[0010] Aspects of the present disclosure relate to a tissue treatment system for providing a cold plasma treatment. A pulser is configured to deliver in use a set of high-voltage pulses to a plasma pad for generating cold plasma of a pre-determined intensity. The pulser comprises a high-voltage power supply for generating electrical energy for the set of high-voltage pulses and a pulse modulation circuit for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses. A geospatial unit is configured to determine geospatial information of the pulser. The geospatial information comprises at least one of a geographical location, an altitude, and an atmospheric pressure. A feedforward controller is configured to perform a initialization of the pulser prior to delivering the set of high-voltage pulses to the plasma pad. The initialization comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses based on a pre-determined relationship between the geospatial information and intensity for generation of cold plasma of the pre-determined intensity that is substantially the same as at the sea level.Advantageously, by initializing the pulser, the cold plasma can be generated independently of the geographical location, altitude, and / or atmospheric pressure.

[0011] By storing the pre-determined relationship in the tissue treatment system, the same system can be initialized at different geographical locations based on the geospatial information. Alternatively, or in addition, the pre-determined relationship can be received in situ from a remote server. By performing a local measurement of the atmospheric pressure relative to a reference remote sensor, the geospatial unit can be kept simple and portable.

[0012] By setting the pulse width of the first pulse, the minimum energy in the pulse required to ignite the cold plasma can be tuned. By setting the pulse repetition frequency of the consecutive pulses, the pre-determined intensity of the cold plasma can be maintained. By providing a ground electrode with a round shape, peak currents can be avoided when collecting current from the tissue resulting from the cold plasma tissue treatment. By providing the system with a user interface, average intensity of the cold plasma can be inputted and then generated independent of the geospatial information.

[0013] Advantageously, a method of generating a cold plasma is employed to provide a cold plasma treatment independent of the geographical location, altitude, and / or atmospheric pressure.

[0014] BRIEF DESCRIPTION OF DRAWINGS

[0015] These and other features, aspects, and advantages of the apparatus, systems and methods of the present disclosure will become better understood from the following description, appended claims, and accompanying drawing wherein:

[0016] FIGs 1A-1B illustrate a system for providing a cold plasma treatment;FIGs 2A-2B illustrate the dependency of cold plasma intensity on pulse width and / or pulse repetition frequency;

[0017] FIGs 3A-3B illustrate the linear relationship between pulse width and / or pulse repetition frequency and the intensity of generated cold plasma;

[0018] FIGs 4A-4B illustrate a plasma pad.

[0019] DESCRIPTION OF EMBODIMENTS

[0020] Terminology used for describing particular embodiments is not intended to be limiting of the invention. As used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. The term "and / or" includes any and all combinations of one or more of the associated listed items. It will be understood that the terms "comprises" and / or "comprising" specify the presence of stated features but do not preclude the presence or addition of one or more other features. It will be further understood that when a particular step of a method is referred to as subsequent to another step, it can directly follow said other step or one or more intermediate steps may be carried out before carrying out the particular step, unless specified otherwise. Likewise it will be understood that when a connection between structures or components is described, this connection may be established directly or through intermediate structures or components unless specified otherwise.

[0021] The invention is described more fully hereinafter with reference to the accompanying drawings, in which embodiments of the invention are shown. In the drawings, the absolute and relative sizes of systems, components, layers, and regions may be exaggerated for clarity.

[0022] Embodiments may be described with reference to schematic and / or crosssection illustrations of possibly idealized embodiments and intermediate structures of the invention. In the description and drawings, like numbers refer to like elements throughout. Relative terms as well as derivativesthereof should be construed to refer to the orientation as then described or as shown in the drawing under discussion. These relative terms are for convenience of description and do not require that the system be constructed or operated in a particular orientation unless stated otherwise.

[0023] FIG 1A illustrates a tissue treatment system 100 for providing a cold plasma treatment. A pulser 102 is configured to deliver in use a set of high-voltage pulses Pp to a plasma pad 101 for generating cold plasma of a pre-determined intensity. The pulser 102 can be connected to the plasma pad 101 via a high-voltage cable 101c and a high voltage pin 10 Ip. The pulser 102 can be connected to a ground pad 105 via a ground pin 105p and a ground cable 105c. In one embodiment, the high-voltage cable 101c and the ground cable 105c are integrated in a single cable, e.g. a coax cable.

[0024] Preferably, the ground pad 105 comprises a body floating electrode.

[0025] Alternatively, or in addition, the ground pad 105 and the pulser 102 are connected to a common ground (earth). The pulser comprises a high-voltage power supply 10 for generating electrical energy for the set of high-voltage pulses Pp and a pulse modulation circuit 20 for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses Pp. In some embodiments, the set of high-voltage pulses Pp may comprise rectangular pulses, sinusoidal pulses, unipolar, or bipolar pulses.

[0026] A geospatial unit 103 comprises program logic for determining geospatial information M of the pulser 102, the geospatial information M comprising at least one of a geographical location, an altitude, and an atmospheric pressure. Preferably, the geospatial unit 103 is located in proximity of the tissue treatment system 100 so that the geospatial information M accurately represents the pressure within the volume of air A between the plasma pad 101 and the wound W. Alternatively, or in addition, the geospatial unit 103 is a remote sensor, and the geospatial information M can be passed on to the tissue treatment system 100 via a data link and / or a user interface. In some embodiments, the geospatial unit 103 comprises atleast one of GPS receiver, Cellular radio, Wi-Fi or local area network card, barometer, pressure sensor, altimeter, and / or other type of sensor configured to determine pressure-dependent geospatial information M. In other or further embodiments, the geospatial information M comprises GPS coordinates, Cell Tower Triangulation, IP address, pressure value, altitude, weather forecast, and / or other kind for information uniquely identifying a geographical location. In one embodiment, the geographical location is defined as an area within which the geospatial information doesn’t change substantially, e.g. area of the substantially same altitude and / or atmospheric pressure.

[0027] A feedforward controller 104 is configured to initialize the pulser 102 prior to delivering the set of high-voltage pulses Pp to the plasma pad 101. In some embodiments, the initialization comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses Pp based on a pre-determined relationship between the geospatial information M and intensity for generation of cold plasma of the predetermined intensity that is substantially the same as at the sea level.

[0028] FIG IB illustrates a schematic drawing of the pulser 102. In some embodiments, the high-voltage power supply 10 comprises a high voltage transformer device T1 including a primary P and secondary S inductor coupled via a magnetic circuit; the secondary inductor S to be coupled to the plasma pad 101 - here schematically indicated as Z_load. A feed circuit of the high-voltage power supply 10 comprises a power source VI, an inductor LI and a unidirectional current conductor DI coupled in series to a power capacitor Cl. The power source VI feeds electrical current into the power capacitor Cl, which is coupled with the primary inductor P and a first controllable conductor Q 1 in series; to provide a pulsed primary current in the primary inductor P resonating with the capacitor Cl when the first controllable conductor Ql is switched in a conducting on-state. If Ql is off, that is, the switch is open in a non conducting off-state, the voltage overcapacitor Cl can be boosted with electrical current. The on / off switching of Q 1 by microcontroller V2 regulates the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses Pp. In one embodiment, the pulse modulation circuit 20 may comprise a pulse wave modulator for controlling the switching of Ql, thereby modulating parameters of the set of high-voltage pulses Pp, e.g. the pulse width, the pulse repetition frequency, and / or a pulse amplitude.

[0029] FIGs 2A-2B illustrate the dependency of cold plasma intensity on a pulse width and / or a pulse repetition frequency. For example, FIG 2 A illustrates the dependency of the intensity I on the pulse width PW for various values of atmospheric pressure ranging from 1015 hectopascals (the sea level), 900 hectopascals (1450m above the sea level), up to 700 hectopascals (3000m above the sea level). In another example, FIG 2B illustrates the dependency of the intensity I on the pulse repetition frequency PRF for various values of atmospheric pressure ranging from 1013 hectopascals (the sea level), 900 hectopascals (1450m above the sea level), up to 700 hectopascals (3000m above the sea level).

[0030] The intensity of cold plasma can be expressed by the amount of power delivered to the generated plasma, by the amount of (visible) light from the generated plasma, and / or by the amount of a specific species present in the generated plasma. Preferably, the intensity of the predetermined and / or generated plasma is normalized, e.g. with respect to the intensity of plasma at sea level or other reference value. Alternatively, or in addition, the intensity is measured in absolute units of i.e. Watt, lux, and / or particle concentration. Preferably, the relationship between the pulse width PW and / or pulse repetition frequency PRF and the generated intensity I of the cold plasma, e.g. as shown in FIGs 2A-2B, is measured under controlled (pressure) conditions in a laboratory setting. Alternatively, or in addition, the relationship is based on a computer simulation.The pulse width can be defined as the duration of time that a pulse remains in its on-state. In one embodiment, it is measured from the point where the rising edge of the pulse reaches half of its maximum amplitude to the point where the falling edge returns to the half of that amplitude. Alternatively, or in addition, other amplitude thresholds can ben envisioned.

[0031] The pulse repetition frequency (PRF) can be defined as the number of pulses transmitted per second by the pulser 102. In one embodiment, the PRF is measured as the number of pulses occurring within a one-second interval. In another or further embodiment, the PRF can be calculated as a reciprocal of a pulse repetition interval (PRI) which is measured as the time between successive pulses.

[0032] In some embodiments, the pre-determined relationship comprises an analytical expression and / or a look-up table relating the geospatial information M to the pulse width and / or the pulse repetition frequency. Typically, the predetermined relationship differs for plasma pad 101 and / or ground pad 105 based on their distinct geometric (e.g. size) or electrical (e.g. conductivity) characteristics. In one embodiment, the pulse width is set based on the geospatial information M in a range from 0.1 microsecond up to 10 000 microseconds, preferably from 1 microsecond up to 1000 microseconds, even more preferably from 10 microseconds up to 500 microseconds. In another or further embodiment, the pulse repetition frequency is set based on the geospatial information M in a range from 10 hertz up to 10000 hertz, preferably in from 100 hertz up to 1000 hertz. For example, the analytical expression may comprise a linear relationship.

[0033] FIGs 3A-3B illustrate the linear relationship between a pulse width, a pulse repetition frequency, and the geospatial information M, e.g. atmospheric pressure P, for generating a pre-determined intensity of cold plasma independent of the geospatial information M. In some embodiments, the pulse width and / or the pulse repetition frequency are increased as alinear function of the atmospheric pressure P. In other or further embodiments, the linear function comprises an independent variable, a dependent variable, a slope and an intercept. In one embodiment, e.g. as shown in FIG 3A, the pre-determined (normalized) intensity is around 70, the independent variable is the pulse width PW (e.g. expressed in microseconds) the dependent variable is the atmospheric pressure P (e.g. expressed in hectopascals), the (unitless) slope is in a range from zero up to one, e.g. 0.0989, and the intercept (e.g. expressed in microseconds) is in a range from zero up to one thousand, e.g. 59.6 microseconds. In another or further embodiment, e.g. as shown in FIG. 3B, the pre-determined (normalized) intensity is around 70, the independent variable is the pulse repetition frequency PRF (e.g. expressed in hertz), the dependent variable is the atmospheric pressure P, the slope is in a range from zero up to one, e.g.

[0034] 0.4, and the intercept is in a range from ten up to one hundred thousand, e.g. 150 hertz.

[0035] FIGs 4A-4B illustrate a plasma pad. In some embodiments, the system 100 comprises a plasma pad 101 configured to be positioned at a wound W. In one embodiment, the plasma pad 101 comprises a high-voltage electrode 10 Ih connected via a high-voltage pin 10 Ip and a high-voltage cable 101c to the pulser 102. The plasma pad 101 comprises a dielectric layer 10 Id arranged between the high-voltage electrode 10 Ih and the wound W. The plasma pad 101 can be formed by a substrate containing a planar high-voltage electrode 10 Ih, that is covered with a dielectric foil or film. An air or gas gap is present in a treatment area formed between an object to be treated, e.g. a foot, functioning as a counter electrode and dielectric with a dielectric constant, e.g. larger than 2. A ground electrode 105g may be placed adjacent to the treatment area. The high-voltage electrode 10 Ih can be made from metal foil, but a mesh is suitably adaptable to the 3D shape of the object to be treated, and will not rupture, crease or fold. The high-voltage electrode 10 Ih may be contacted by a high-voltage cable 101c, thatconnects to a high-voltage pin 10 Ip. The ground electrode 105g may be contacted by a ground cable 105c, that connects to a ground pin 105p. The high-voltage electrode 10 Ih covered with the dielectric layer 10 Id is in contact with the object to be treated, e.g. skin of the foot.

[0036] In other or further embodiments, the dielectric layer 10 Id comprises dielectric structures configured to trap ambient air A in a volume between the dielectric layer 10 Id and the wound W. If the electric field is high enough >30 kV / cm and if the thickness of the air gap is rather constant, homogeneously distributed cold plasma discharges are created in the air gap to the object to be treated e.g. the skin of a foot. Dielectric and protrusions have a high dielectric strength, e.g. > 180 kV / mm.

[0037] In some embodiments, the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses Pp are configured to ignite and maintain the cold plasma in the volume of air A between the dielectric layer 10 Id and the wound W. The strength of the electric field in the volume of air A is dependent on the energy stored in the set of high-voltage pulses Pp delivered by the pulser to the high-voltage electrode 10 Ih of the plasma pad 101. When the energy is high enough to generate electric field higher than the dielectric strength of the air A in the volume between the dielectric layer 10 Id and the wound W, cold plasma is ignited in the volume of air A. The energy stored in the set of high-voltage pulses Pp can preferably be controlled by modifying the pulse width and / or the pulse amplitude. After the ignition, the cold plasma is maintained by consecutive pulses repeatedly delivered to the high-voltage electrode 10 Ih of the plasma pad 101. The intensity of the cold plasma to be maintained can preferably be controlled by the pulse repetition frequency.

[0038] Preferably, the atmospheric pressure determined by the geospatial unit 103 is representative of the pressure in the volume of air A between the plasma pad 101 and the wound W. Alternatively, or in addition, the atmospheric pressure of the volume of air between the plasma pad 101and the wound W is determined indirectly using the geospatial unit 103. In some embodiments, the geospatial unit 103 is configured to perform local measurement of the geospatial information M. The local measurement can be adjusted and / or related to an atmospheric pressure measurement performed by a remote reference sensor. For example, the geospatial unit 103 is a capacitive sensor configured to measure pressure relative to a pressure of gas in an enclosed chamber. The local (relative) measurement is then adjusted (calibrated) based on an absolute measurement of the gas pressure carried out at a remote location by a remote reference pressure sensor configured to measure absolute value of pressure inside the enclosed chamber. In another example, the geospatial unit 103 is a GPS receiver configured to measure geographical location of the pulser 102 and the remote reference sensor is a barometer (e.g. a weather station) configured to measure atmospheric pressure at and / or nearby the geographical location of the pulser. Other combinations of local geospatial information M measurements by the geospatial unit 103 of the pulser and reference measurements performed by remote reference sensors can be envisioned.

[0039] In other or further embodiments, the system 100 comprises a ground pad 105 of a round shape configured to establish uniform and conformal electrical interface with the tissue (T) surrounding the wound (W) for collecting electrical current resulting from the cold plasma treatment of the tissue (T). Preferably, the diameter of the ground pad 105 is in a range from 3 centimeter up to 9 centimeter, more preferably at least 4 centimeter, the most preferably at least 5 centimeter. The ground pad is preferably electrically connectable to a reference potential, e.g. earth potential which may be provided in the pulser. In one embodiment, e.g. as shown in FIGs 4A-4B, the diameter of the ground pad 105 adheres with substantially the whole surface area to the tissue (T) forming the ground electrode 105g. Such an enlarged area is advantageous to minimize a risk of cauterization due to electrical currents flowing through the tissue to the ground pad 105.Preferably, the ground pad 105 is formed of a flexible material that can conform to the curvature of the tissue T. Alternatively, or in addition, the ground pad 105 can be split into multiple segments to conform to the tissue T. In some embodiments, the ground pad 105 is arranged on an opposite side of the object to be treated, e.g. as shown in FIG 1A, for generating cold plasma in a volume of air under the plasma pad 101 above the wound W. In other or further embodiments, the ground pad 105 is placed adjacent to the plasma pad 101, e.g. as shown in FIG 4A, for generating volume plasma in the volume of air around the wound W (volume plasma generation). In yet another or further embodiment, the ground electrode 105g is integrated in the plasma pad 101, e.g. as shown in FIG 4B, for generating plasma above the surface of the wound W (surface plasma generation). In some embodiments, the ground pad 105 comprises electrically conductive adhesive configured to adhere the ground pad 105 to the tissue T. In one embodiment, the strength of the adhesive is configured to support continuous adhesion of the ground electrode 105g to the tissue T during the cold plasma treatment.

[0040] In some embodiments, the system 100 comprises a user interface 102u for inputting an average intensity of the cold plasma to be generated independently of the geospatial information M. In one embodiment, the pulser 102 is initialized by the feedforward controller 104 based on the average intensity of the cold plasma and at least one linear relationship between the pulse width and / or the pulse repetition frequency, and the geospatial information M.

[0041] In other or further embodiments, a method of generating a cold plasma is utilized for delivering a cold plasma treatment of a wound W. The method comprises the step of providing a pulser 102 configured to deliver in use a set of high-voltage pulses Pp to a plasma pad 101 for generating cold plasma of a pre-determined intensity. The pulser comprises a high-voltage power supply 10 for generating electrical energy for the set of high-voltagepulses Pp and a pulse modulation circuit 30 for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses Pp. In one embodiment, the method comprises the step of determining, by a geospatial unit 103, geospatial information M of the pulser 102, the geospatial information M comprising at least one of a geographical location, an altitude, and an atmospheric pressure. In another or further embodiment, the method comprises the step of initializing, by a feedforward controller 104, the pulser 102 prior to delivering the set of high-voltage pulses Pp to the plasma pad 101. In another or further embodiment, the initialization comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses Pp based on a pre-determined relationship between the geospatial information M and cold plasma intensity for generation of cold plasma of the pre-determined intensity that is substantially the same as at the sea level. In other or further embodiments, the pre-determined relationship comprises an analytical expression and / or a look-up table relating the geospatial information M to the pulse width and / or the pulse repetition frequency.

[0042] In interpreting the appended claims, it should be understood that the word "comprising" does not exclude the presence of other elements or acts than those listed in a given claim; the word "a" or "an" preceding an element does not exclude the presence of a plurality of such elements; any reference signs in the claims do not limit their scope; several "means" may be represented by the same or different item(s) or implemented structure or function; any of the disclosed devices or portions thereof may be combined together or separated into further portions unless specifically stated otherwise.

Claims

CLAIMS1. A tissue treatment system (100) for providing a cold plasma treatment, the system (100) comprisinga pulser (102) configured to deliver in use a set of high-voltage pulses (Pp) to a plasma pad (101) for generating cold plasma of a predetermined intensity, the pulser comprisinga high-voltage power supply (10) for generating electrical energy for the set of high-voltage pulses (Pp);a pulse modulation circuit (20) for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses (Pp);a geospatial unit (103) configured to determine geospatial information (M) of the pulser (102), the geospatial information (M) comprising at least one of a geographical location, an altitude, and an atmospheric pressure; anda feedforward controller (104) configured to perform a initialization of the pulser (102) prior to delivering the set of high-voltage pulses (Pp) to the plasma pad (101);wherein the initialization comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses (Pp) based on a pre-determined relationship between the geospatial information (M) and intensity for generation of cold plasma of the pre-determined intensity that is substantially the same as at the sea level.

2. The system (100) according to the preceding claim, wherein the predetermined relationship comprises an analytical expression and / or a look-up table relating the geospatial information (M) to the pulse width and / or the pulse repetition frequency.

3. The system (100) according to any of the preceding claims, wherein the pulse width is set in a range from 1 microsecond to 1000 microseconds based on the geospatial information (M).

4. The system (100) according to any of the preceding claims, wherein the pulse repetition frequency is set in a range from 10 hertz up to 100 kilohertz based on the geospatial information (M).

5. The system (100) according to any of the preceding claims, wherein the pulse width and / or the pulse repetition frequency are increased as a monotonic function of atmospheric pressure.

6. The system (100) according to any of the preceding claims, wherein the system (100) comprises a plasma pad (101) configured to be positioned at a wound (W), the plasma pad (101) comprisinga high-voltage electrode (10 Ih) connected to the pulser (102);a dielectric layer (10 Id) arranged between the high-voltage electrode (10 Ih) and the wound (W).

7. The system (100) according to the preceding claim, wherein the dielectric layer (10 Id) comprises dielectric structures configured to trap ambient air (A) in a volume between the dielectric layer (10 Id) and the wound (W).

8. The system (100) according to any of the two preceding claims, wherein the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses (Pp) are configured to ignite and maintain a cold plasma in a volume of air (A) between the dielectric layer (10 Id) and the wound (W).

169. The system (100) according to any of the three preceding claims, wherein the atmospheric pressure determined by the geospatial unit (103) is representative of the pressure in the volume of air (A) between the plasma pad (101) and the wound (W).

10. The system (100) according to any of the preceding claims, wherein the geospatial unit (103) is configured to perform local measurement of the geospatial information (M) and wherein the local measurement is adjusted and / or related to an atmospheric pressure measurement performed by a remote reference sensor.

11. The system (100) according to any of the preceding claims comprising a flexible ground pad (105) of a rounded shape for collecting electrical current resulting from the cold plasma tissue treatment, wherein the flexible ground pad (105) comprises electrically conductive adhesive over substantially its whole surface area suited to adhering the ground pad (105) to the tissue (T).

12. The system (100) according to the preceding claim, wherein the ground pad (105) is at least five centimeter in diameter and is electrically connectable to the pulser.

13. The system (100) according to any of the preceding claims, wherein the system (100) comprises a user interface (102u) for inputting an average intensity of the cold plasma to be generated independently of the geospatial information (M).

14. The system (100) according to the preceding claim, wherein the pulser (102) is initialized by the feedforward controller (104) based on the average intensity of the cold plasma and at least one linear relationship between the pulse width and / or the pulse repetition frequency, and the geospatial17information (M).

15. A method of generating a cold plasma, the method comprising providing a pulser (102) configured to deliver in use a set of high- voltage pulses (Pp) to a plasma pad (101) for generating cold plasma of a pre-determined intensity, the pulser comprising a high-voltage power supply (10) for generating electrical energy for the set of high-voltage pulses (Pp);a pulse modulation circuit (30) for controlling pulse width and / or pulse repetition frequency of the set of high-voltage pulses (Pp);determining, by a geospatial unit (103), geospatial information (M) of the pulser (102), the geospatial information (M) comprising at least one of a geographical location, an altitude, and an atmospheric pressure; andinitializing, by a feedforward controller (104), the pulser (102) prior to delivering the set of high-voltage pulses (Pp) to the plasma pad (101);wherein the step of initializing comprises setting the pulse width and / or the pulse repetition frequency of the set of high-voltage pulses (Pp) based on a pre-determined relationship between the geospatial information (M) and the intensity for generation of cold plasma of the pre-determined intensity that is substantially the same as at the sea level.

16. The method according to the previous claim, wherein the predetermined relationship comprises an analytical expression and / or a look-up table relating the geospatial information (M) to the pulse width and / or the pulse repetition frequency.

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