5results about How to "Avoid purification steps" patented technology

The present invention belongs to the field of biotechnology, and relates to a hybridoma cell strain secreting a monoclonal antibody of Clostridium perfringens CPA with neutralizing activity and its application. The present invention uses natural CPA as an immune antigen and a non-toxic CPA mutant as a coating antigen for the first time, and screens a hybridoma cell strain secreting a monoclonal antibody with neutralizing activity. The hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity, and can achieve large-scale batch production, thereby providing a material basis for the detection and treatment of CPA antigen / antibody in the later stage.

This invention relates to a pyridine-imine type metal complex, its preparation method, and its applications. The pyridine-imine type metal complex is an NNN-pyridine-imine type metal complex or an NN-pyridine-imine type metal complex; its structural formula is shown in Formulas 1-4. The pyridine-imine type metal complex and its preparation method described in this invention, as well as its application in hydrosilylation and silanization reactions, allow for the one-pot synthesis of the desired metal complex. The preparation method is simple and low-cost, eliminating the cumbersome separation steps in traditional two-step methods, significantly reducing reaction time, increasing the maximum yield to over 95%, and exhibiting high catalytic activity and selectivity in hydrosilylation and silanization reactions.

The present invention provides a preparation method of high-purity trimetazidine hydrochloride, and the steps include: S1, adding raw materials 2,3,4-trimethoxybenzaldehyde and anhydrous piperazine to an ionic liquid, adding an asymmetric catalyst, and performing microwave radiation heating to obtain a mixed solution; S2, the mixed solution is subjected to supercritical fluid extraction, cooled to room temperature, and an extract is obtained; S3, the extract is added to an organic solvent, hydrochloric acid is added and mixed evenly, the temperature is lowered and crystallized, suction filtration is washed, and dried, crushed and sieved to obtain high-purity trimetazidine hydrochloride. The preparation process of the present invention adopts microwave radiation technology and supercritical fluid extraction technology to improve the yield and purity of trimetazidine hydrochloride, and the obtained trimetazidine hydrochloride yield is as high as 95%, the purity is as high as 99.95%, the production efficiency is high, the cost is low, the product quality is stable, and it has a good market prospect.

The application belongs to the technical field of sodium hexafluorophosphate and particularly relates to a one-pot preparation process of sodium hexafluorophosphate. The one-pot preparation process of sodium hexafluorophosphate comprises the following steps: (1) reaction: anhydrous hydrogen fluoride is put into a reactor, a sodium source and a phosphorus source are sequentially added at a temperature of -60 to -20 DEG C, then the temperature is increased to 0 to 10 DEG C for reaction, volatile substances are evaporated after the reaction is completed, and a sodium hexafluorophosphate primary product is obtained; (2) neutralization: an organic solvent is added to the sodium hexafluorophosphate primary product for dissolution, then an alkaline substance is added to adjust the pH value of the solution system to 7 to 9, filtration is performed, a sodium hexafluorophosphate solution is obtained, the organic solvent is evaporated by increasing the temperature, and a sodium hexafluorophosphate crude product is obtained after drying. The one-pot preparation process has the advantages of mild conditions, simple operation, fast reaction rate, easy separation of the product, high purity and high yield of the prepared sodium hexafluorophosphate crude product, and is suitable for industrial production.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of selecting natural toxins, an indirect ELISA detection method is established by utilizing nontoxic ETX single amino acid site protein mutants, a series of hybridoma cell strains secreting anti-clostridium perfringens ETX monoclonal antibodies are screened, and the hybridoma cell strains can stably and efficiently secrete the monoclonal antibodies with neutralizing activity. According to the present invention, the clostridium perfringens ETX monoclonal antibody with neutralization resistance is combined with the ETX tetraamino acid mutant to establish the ETX antibody blocking ELISA detection method, and the method can reduce the potential safety hazard of the ETX natural toxicity and the potential safety hazard of the single amino acid mutant amino acid reverse mutation; the kit also has the characteristics of simplicity and convenience in operation, low cost, quick reaction, strong specificity and the like, and can provide reference for diagnosis of clostridium perfringens disease.