10results about How to "Avoid purification steps" patented technology

The invention discloses a preparation method of ultra-pure 4, 4 '-biphenol, which comprises the following steps: in the presence of air and a first catalyst, carrying out a coupling reaction by using 2, 6-di-tert-butyl phenol as a raw material to obtain 3, 3', 5, 5 '-tetra-tert-butyl-4, 4'-biphenol; and adding a second catalyst into the 3, 3 ', 5, 5'-tetra-tert-butyl-4, 4 '-biphenol, carrying out a heating reaction, after the reaction is finished, filtering to obtain a 4, 4'-biphenol crude product, and recrystallizing and drying to obtain the ultra-pure 4, 4 '-biphenol, the preparation method disclosed by the invention is mild in reaction condition, simple and convenient in post-treatment, easy to operate and excellent in yield, and the product purity reaches 99.99% or above.

The present invention belongs to the field of biotechnology, and relates to a hybridoma cell strain secreting a monoclonal antibody of Clostridium perfringens CPA with neutralizing activity and its application. The present invention uses natural CPA as an immune antigen and a non-toxic CPA mutant as a coating antigen for the first time, and screens a hybridoma cell strain secreting a monoclonal antibody with neutralizing activity. The hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity, and can achieve large-scale batch production, thereby providing a material basis for the detection and treatment of CPA antigen / antibody in the later stage.

The invention relates to a triazolotriazinyl fused ring nitrogen-rich organic covalent skeleton as well as a preparation method and application thereof. The structure of the triazolo-triazinyl fused ring nitrogen-rich organic covalent skeleton is as shown in a formula I in the specification. The invention also provides a preparation method of the triazolo triazinyl fused ring nitrogen-rich organic covalent skeleton, which comprises the following steps: polymerizing a 2, 6, 10-triformonitrile-tri [1, 2, 4] triazolo [1, 5-a: 1 ', 5'-c: 1 '', 5''-e] [1, 3, 5] triazine compound and a catalyst under the conditions of heating and inert gas to obtain a crude product; and placing the crude product in an acid solution or an organic solvent, and filtering to obtain the triazolotriazinyl fused ring nitrogen-rich organic covalent skeleton. The invention also provides an application of the triazolotriazinyl fused ring nitrogen-rich organic covalent skeleton as a photocatalyst. The invention provides a novel triazolotriazinyl fused ring nitrogen-rich organic covalent skeleton, so that a novel photocatalyst is provided. # imgabs0 #

This invention relates to a pyridine-imine type metal complex, its preparation method, and its applications. The pyridine-imine type metal complex is an NNN-pyridine-imine type metal complex or an NN-pyridine-imine type metal complex; its structural formula is shown in Formulas 1-4. The pyridine-imine type metal complex and its preparation method described in this invention, as well as its application in hydrosilylation and silanization reactions, allow for the one-pot synthesis of the desired metal complex. The preparation method is simple and low-cost, eliminating the cumbersome separation steps in traditional two-step methods, significantly reducing reaction time, increasing the maximum yield to over 95%, and exhibiting high catalytic activity and selectivity in hydrosilylation and silanization reactions.

The present invention provides a preparation method of high-purity trimetazidine hydrochloride, and the steps include: S1, adding raw materials 2,3,4-trimethoxybenzaldehyde and anhydrous piperazine to an ionic liquid, adding an asymmetric catalyst, and performing microwave radiation heating to obtain a mixed solution; S2, the mixed solution is subjected to supercritical fluid extraction, cooled to room temperature, and an extract is obtained; S3, the extract is added to an organic solvent, hydrochloric acid is added and mixed evenly, the temperature is lowered and crystallized, suction filtration is washed, and dried, crushed and sieved to obtain high-purity trimetazidine hydrochloride. The preparation process of the present invention adopts microwave radiation technology and supercritical fluid extraction technology to improve the yield and purity of trimetazidine hydrochloride, and the obtained trimetazidine hydrochloride yield is as high as 95%, the purity is as high as 99.95%, the production efficiency is high, the cost is low, the product quality is stable, and it has a good market prospect.

The invention discloses a tumor targeting activatable nano photosensitizer as well as a preparation method and application thereof. The tumor targeting activatable nano photosensitizer comprises a cationic polymer, an anionic polymer and an aromatic structure photosensitizer. Cationic-pi interaction is formed between a cationic side chain in the cationic polymer and the photosensitizer with an aromatic structure, the nano photosensitizer is formed through self-assembly under the interaction, and the photosensitive activity of the nano photosensitizer in blood circulation is in a completely inactivated state; and no light toxic or side effect is generated on normal light exposure tissues. When selectively enriched in tumor tissues, the photosensitizer is structurally degraded in tumor cells in a specific environment, and the photosensitive activity of the photosensitizer is quickly activated and recovered, so that efficient tumor inhibition treatment is realized.

The application belongs to the technical field of sodium hexafluorophosphate and particularly relates to a one-pot preparation process of sodium hexafluorophosphate. The one-pot preparation process of sodium hexafluorophosphate comprises the following steps: (1) reaction: anhydrous hydrogen fluoride is put into a reactor, a sodium source and a phosphorus source are sequentially added at a temperature of -60 to -20 DEG C, then the temperature is increased to 0 to 10 DEG C for reaction, volatile substances are evaporated after the reaction is completed, and a sodium hexafluorophosphate primary product is obtained; (2) neutralization: an organic solvent is added to the sodium hexafluorophosphate primary product for dissolution, then an alkaline substance is added to adjust the pH value of the solution system to 7 to 9, filtration is performed, a sodium hexafluorophosphate solution is obtained, the organic solvent is evaporated by increasing the temperature, and a sodium hexafluorophosphate crude product is obtained after drying. The one-pot preparation process has the advantages of mild conditions, simple operation, fast reaction rate, easy separation of the product, high purity and high yield of the prepared sodium hexafluorophosphate crude product, and is suitable for industrial production.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of natural toxin, an indirect ELISA (enzyme-linked immunosorbent assay) detection method is established by utilizing a non-toxic ETX single amino acid site protein mutant, and a series of hybridoma cell strains secreting an anti-clostridium perfringens ETX monoclonal antibody are screened; the hybridoma cell strain can stably and efficiently secrete a monoclonal antibody with neutralizing activity and a monoclonal antibody without neutralizing resistance respectively, and large-scale batch production can be realized. According to the present invention, the clostridium perfringens ETX monoclonal antibody is firstly utilized to establish the ETX antigen ELISA detection method, and the method has characteristics of simple sample operation, low cost, rapid reaction, strong specificity and the like, can provide the reference for the diagnosis of clostridium perfringens, and provides the basis for the research of the related vaccine potency test substitution method.

The invention belongs to the technical field of biology, and particularly relates to a hybridoma cell strain capable of secreting a clostridium perfringens ETX monoclonal antibody with neutralizing activity and application of the hybridoma cell strain. On the basis of selecting natural toxins, an indirect ELISA detection method is established by utilizing nontoxic ETX single amino acid site protein mutants, a series of hybridoma cell strains secreting anti-clostridium perfringens ETX monoclonal antibodies are screened, and the hybridoma cell strains can stably and efficiently secrete the monoclonal antibodies with neutralizing activity. According to the present invention, the clostridium perfringens ETX monoclonal antibody with neutralization resistance is combined with the ETX tetraamino acid mutant to establish the ETX antibody blocking ELISA detection method, and the method can reduce the potential safety hazard of the ETX natural toxicity and the potential safety hazard of the single amino acid mutant amino acid reverse mutation; the kit also has the characteristics of simplicity and convenience in operation, low cost, quick reaction, strong specificity and the like, and can provide reference for diagnosis of clostridium perfringens disease.

The invention relates to a saccharomyces cerevisiae strain and application thereof in efficient synthesis of beta-NMN, and belongs to the technical field of bioengineering. The strain is saccharomyces cerevisiae Y1t, is classified and named as saccharomyces cerevisiae, and is preserved in China General Microbiological Culture Collection Center (CGMCC) on March 26, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.30138. The invention further discloses a preparation method of the saccharomyces cerevisiae. The strain can catalyze conversion of nicotinamide ribose into beta-NMN through one-step reaction, the purification step of enzyme liquid is omitted, and an enzymatic reaction system with higher stability and catalytic efficiency is realized by utilizing a surface immobilized enzyme technology; in addition, beta-NMN can be efficiently synthesized through the strain, powerful technical support is provided for industrial large-scale production of beta-NMN, and influences can be possibly generated on related health products, medicine and food industries. The production cost of the beta-NMN is reduced, and the application prospect of the beta-NMN in the fields of cell energy metabolism, anti-aging research and related medical treatment is widened.