The invention discloses a
cell DNA damage detection method, and relates to the
cell damage detection. The
cell DNA damage detection method comprises the following steps: preheating a to-be-detected cell sample with low-gelling temperature
agarose prepared from
calcium and
magnesium-free PBS (
Phosphate Buffer Solution) to obtain a mixed liquid, paving the mixed liquid in a plate cover sample hole of a 24-hole cell culturing plate, spreading
agarose evenly, cooling, curing, and then immersing the plate cover into cell
lysis buffer, and pyrolyzing; washing an
agarose plate after
pyrolysis by an alkaline unwinding liquid, and then immersing the agarose plate into a container filled with a precooling alkaline unwinding liquid; placing the agarose plate after alkaline unwinding into an
electrophoresis tank filled with a precooling
electrophoresis liquid for
electrophoresis; immersing the agarose plate after the electrophoresis into milli-Q water; standing and immersing the agarose plate by precooling absolute ethyl
alcohol, and refrigerating and storing the taken agarose plate; adding a drop of MillI-Q water dropwise in each hole of the agarose plate after
dehydration and
drying, standing,
wetting the agarose plate, adding 10-15micron L of
ethidium bromide dropwise in each hole, keeping away from light,
dyeing, sucking residual dye liquid dry on the surface of the agarose plate by
filtration paper, and collecting images with
excitation wavelength being 515nm.