The invention relates to a method for rapidly evaluating in-vitro antagonistic
helicobacter pylori activity, and belongs to the technical field of microbiological detection. The method comprises the following steps of taking a
sheep blood agar culture medium plate,
coating the
sheep blood agar culture medium plate with a bacterial suspension of
helicobacter pylori, placing an oxford cup, adding ato-be-detected sample solution into the oxford cup, culturing for 3-5d, and measuring the
diameter of an
inhibition zone; and preparing to-be-detected sample solutions with different concentrations, respectively adding the to-be-detected sample solutions into 96-well plates, adding the
sheep blood agar culture medium into each well while the goat blood agar culture medium is hot, mixing, adding the bacterial suspension of the
helicobacter pylori into each well after the sheep blood agar culture medium is solidified, culturing for 3-5d, and determining the minimum inhibition concentration of the to-be-detected sample solutions on the
helicobacter pylori. The method has the characteristics of simplicity, high efficiency, short judgment time,
visual observation result and the like, meets therequirements of a
helicobacter pylori in-vitro
antagonism experiment method, and is suitable for multi-sample detection.