38results about How to "Short detection process" patented technology

The invention belongs to an infrared dim-small target detection method based on shear wave conversion in the infrared image processing technology. The method comprises the following steps: processing an original infrared image by employing nonsubsampled Laplace pyramid conversion and a Shearlet filter in succession to obtain high frequency information graphs of various directions under different scales, inhibiting background and noise interference information, enhancing target information and extracting a dim-small target. According to the invention, the nonsubsampled Laplace pyramid conversion and a Shearlet filter are employed to process the original infrared image, through same scale and different scales fusion processing of the obtained the high frequency information graphs, the interference information is inhibited, the target information is enhanced, and the high frequency information graphs are subjected to segmentation to obtain a clear dim-small target graph; thereby the method has the characteristics of a short detection processing flow, small data processing amount, short processing time, capability of effectively raising performance of detecting the infrared dim-small target and obviously distinguishing a target and a complex background in the image, a good effect and the like.

The invention provides a method for inspecting the acceptability of amorphous alloy products by means of sound, which includes the following specific steps: an amorphous alloy product is impacted to vibrate and emit sound under the condition of no contact with other objects; the sound signals of the amorphous alloy product, which are generated by impact, are acquired; the sound signals are processed, and the attenuation time of the sound signals is counted; and according to the length of the attenuation time of the sound signals, the acceptability of the product is judged. The method can be used for accurately inspecting whether shrunk holes, cracks or other defects exist in the amorphous alloy product, inspection can be automatically carried out as long as the threshold of the attenuation time of the sound signals of an acceptable product is set in advance, human subjective judgement is not needed, so that misjudgement can be reduced, moreover, the inspection flow is short, and the method is suitable for the inspection of products in large batches.

The invention relates to the field of molecular biology, in particular to a nucleic acid signal amplification sequence and a nucleic acid signal amplification method. The nucleic acid signal amplification sequence comprises a leader sequence and a repetitive sequence in a plurality of times; the leader sequence and an objective target drone sequence complement each other; and the repetitive sequence and a reporter probe complement each other. An objective target drone can be captured and hybridized by the leader sequence of the nucleic acid signal amplification sequence, and then by the repetitive sequence in a plurality of times and corresponding reporter probes, signals can be amplified, so that nucleic acid signals of the objective target drone are detected. Compared with a PCR technology which needs enzyme to maintain a hybrid system, the nucleic acid signal amplification sequence can detect nucleic acid substances on the premise of not increasing concentration of a detected object, and is good in stability, high in repeatability, low in cost and short in detection technological process.

The invention provides a kit for detecting mitochondrial DNA T4353C mutation linked to primary hypertension. The kit is composed of DNA extraction mixed liquor, PCR mixed liquor, outer primers and inner primers specifically designed for T4353C, restriction endonuclease, a positive control, a negative control and a kit body. According to the invention, genomic DNAs are rapidly extracted from samples like a small amount of a blood specimen, hair with hair follicles, a mouth mucosa scraping smear and saliva by using a protease K digestion cleavage process, so problems in extraction of DNAs from blood of a patient with primary hypertension in the prior art are overcome, pains of a detected person are alleviated, and trans-regional sample transmission is realized; different sites formed by the specific restriction endonuclease enable specificity of enzyme digestion to be improved; the phenomenon of false negative results is avoided, and stability of detection results is improved; and the kit has the advantages of low detection cost, simple and fast detection flow, visually interpretable results and good practicality.

The invention provides a gene detection method of Leber's hereditary optic neuropathy, (LHON), a gene chip and a kit. The kit comprises a plurality of PCR probes and primers for detecting SNP of optic nerve lesion genes. The chip is provided with detection probes aiming at 13 mitochondrial genes and 46 SNP loci. According to the gene detection method, the kit and the gene chip are utilized to perform detection, the gene detection method comprises the following steps: performing amplification and hybridization on marked samples; scanning hybridization signals; obtaining chip data, and processing the obtained chip data; judging SNP loci, and judging and reading SNP loci information of the visual lesion genes. The gene detection method disclosed by the invention overcomes the detects that by adopting the direct sequencing, the using sample volume is large and the blood sample quantity is large, the amplification time is long, the amplification frequency is high, and false positive or false negative results can be easily caused, and the like, so that the pain of detected persons can be relieved, and not only be the detection accuracy improved, but also the detection time is shortened. According to the invention, the chip and the kit which have the characteristics of being rapid and being high in flux are provided, so that the early diagnosis and treatment of diseases can be facilitated, and nationwide large-scale screening and preventive inspection are convenient to carry out.

The invention relates to a rapid simultaneous detection method for the content of copper, zinc and iron in gold mud. A sample is dissolved by utilizing nitric acid, aqua regia, sulfuric acid and nitric-sulfuric acid; after sulfuric acid is added for smoking, a copper-zinc-iron compound is decomposed to form a corresponding oxide, HAuCl4 is decomposed to form elemental gold, and H<n-1>[AgCln]<-n+1> is decomposed to form silver chloride; after sulfuric acid is added for dissolving salts, the copper iron zinc oxide is dissolved to form sulfate; after ammonia water is added, silver-copper-zinc is transformed into corresponding ammonia complex ions, iron is transformed into iron hydroxide precipitation, and gold and iron are filtered and then separated from filtrate; after the pH value of the filtrate is adjusted by adding sulfuric acid and ammonia water, sodium chloride is added for precipitation of silver, the silver is recovered by filtering, copper in the filtrate is detected by adopting iodometry, and zinc is detected by adopting an EDTA method; and iron is detected by using a potassium dichromate method. The sample consumption is less in a detection process, precious metals can be effectively classified and recovered, the detection process is simplified, the energy consumption is reduced, and the detection efficiency is improved.

The invention provides a kit for detecting deaf related mitochondrial DNA T7505C mutation. The kit is composed of a DNA extraction mixed liquid, a PCR mixed liquid for T7505C fragment amplification, a pair of outer primers designed against T7505C, a pair of inner primers designed against the T7505C, a restrictive endonuclease, a positive contrast, a negative contrast and a kit body. A protease K digestion cracking method is utilized in the invention to rapidly extract genome DNA from a small amount of specimens, so a problem of the traditional extraction of DNA from the blood of a deaf patient is solved, the pains of a detected person is mitigated, and a trans-regional sample transmission problem is also solved. The kit has the advantages of low application cost, simple and rapid detection flow, visual result interpretation, ensuring of the specificity and the stability of the detection result, and realization of the application in the detection of the deaf related mitochondrial tRNAGlnT7505C mutation.

The invention provides a kit for detecting mitochondrial DNA T5655C mutation linked to primary hypertension. The kit is composed of DNA extraction mixed liquor, PCR mixed liquor, outer primers and inner primers specifically designed for T5655C, restriction endonuclease, a positive control, a negative control and a kit body. According to the invention, genomic DNAs are rapidly extracted from samples like a small amount of a blood specimen, hair with hair follicles, a mouth mucosa scraping smear and saliva by using a protease K digestion cleavage process, so problems in extraction of DNAs from blood of a patient with primary hypertension in the prior art are overcome, pains of a detected person are alleviated, and trans-regional sample transmission is realized; different sites formed by the specific restriction endonuclease enable specificity of enzyme digestion to be improved; molecular internal control indexes are set, so the phenomenon of false negative results is avoided and stability of detection results is improved; and the kit has the advantages of low detection cost, simple and fast detection flow, visually interpretable results and good practicality.

The invention relates to a single-particle-based LIBS and Raman spectrum aerosol online detection device, wherein the aerodynamic lens is used to focus the atmospheric aerosol particle sample into a collimated particle beam and screen the particle size, an oscilloscope, two continuous The laser and the corresponding photomultiplier tube together constitute a dual-beam caliper system. The first and second Nd:YAG lasers interact with particles to excite Raman spectroscopy and LIBS spectroscopy respectively. The element composition information in aerosol particles can be detected in real time through LIBS spectroscopy, and the characteristic spectral lines of each element belong to atomic spectral lines without overlapping interference. Raman spectroscopy studies the vibrational and rotational spectra of molecules to obtain structural information on aerosol particles and to obtain information on lattice vibrations of particle samples. The device of the invention is simple and compact in structure, has low requirements on instruments, only needs a low vacuum environment to eliminate background noise interference, and reduces the cost of the detection device. The detection process of the device of the invention is short, and the analysis result can be obtained after a single pulse laser.