38results about How to "Short detection process" patented technology

The invention belongs to an infrared dim-small target detection method based on shear wave conversion in the infrared image processing technology. The method comprises the following steps: processing an original infrared image by employing nonsubsampled Laplace pyramid conversion and a Shearlet filter in succession to obtain high frequency information graphs of various directions under different scales, inhibiting background and noise interference information, enhancing target information and extracting a dim-small target. According to the invention, the nonsubsampled Laplace pyramid conversion and a Shearlet filter are employed to process the original infrared image, through same scale and different scales fusion processing of the obtained the high frequency information graphs, the interference information is inhibited, the target information is enhanced, and the high frequency information graphs are subjected to segmentation to obtain a clear dim-small target graph; thereby the method has the characteristics of a short detection processing flow, small data processing amount, short processing time, capability of effectively raising performance of detecting the infrared dim-small target and obviously distinguishing a target and a complex background in the image, a good effect and the like.

The invention provides a kit for simultaneously detecting mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and a using method thereof. The kit comprises a reagent for extracting sample genomic DNA, a PCR amplification reactive reagent, a primer mixed liquor, a positive control template and a negative control template. The using method of the kit mainly comprises the following steps: using blood, hair with follicle, oral mucosa doctor blade, saliva, and the like, as a sample; adopting a proteinase K digestion pyrolysis method to extract genomic DNA; and then simultaneously detecting mutations in A1555G and C1494T by multiple allele specific PCR. The kit is used for detecting the mutations in mitochondria DNA A1555G and C1494T related to maternally inherited drug-induced deafness and is more rapid, economical and simpler than the single detection for mutation in A1555G or C1494T, and the kit has low requirements for equipment and environment and is conducive to promotion and application.

The invention discloses a method for detecting the dispersibility of titanium white in plastics, and belongs to the field of chemical industry. In order to solve the technical problem, the invention provides a simple and reliable method for detecting the dispersibility of titanium white in plastics. The method comprises the following steps of: a) fully mixing the titanium white and a resin to obtain a material to be detected; and b) extruding the material to be detected in an extruder, burning the material in different extrusion sections at resin decomposing temperature, calculating the solid content of the material in different extrusion sections, and characterizing the dispersibility of the titanium white in the plastics by using a ratio of the solid content, wherein the solid content is a quotient obtained by the mass of the material before burning by the mass of the material after burning. The dispersibility of the titanium white in the plastics is characterized by the ratio of the solid content, and the method has the advantages of high operability, simplicity, practicability, short detection flow, and high detection accuracy and suitable to be promoted and applied in the field.

The invention relates to an LIBS and Raman spectrum aerosol online detection device based on a single particle. An aerodynamic lens is used for focusing an atmospheric aerosol particle sample into a collimated particle beam and screening a particle size, and an oscilloscope, two continuous lasers and corresponding photomultipliers jointly form a double-beam diameter measuring system. And a first Nd: YAG laser and a second Nd: YAG laser act with particulate matters to excite a Raman spectrum and an LIBS spectrum. Element composition information in aerosol particles can be detected in real timethrough the LIBS spectrum, characteristic spectral lines of all elements belong to atomic spectral lines, and overlapping interference is avoided. The Raman spectrum studies vibration and rotation spectrum of molecules to obtain structure information of aerosol particles, and obtains lattice vibration information of a particle sample. The device is simple and compact in structure and low in instrument requirement, background noise interference is eliminated only through a low-vacuum environment, and cost of the detection device is reduced. The device is short in detection process, and an analysis result can be obtained after a single pulse laser.

The invention provides a diagnostic kit for obesity gene mutation. Probe sequences are designed according to the principle of sequence reverse complementation in the direction from 5' to 3' specific to the coding sequence of obesity-related pathogenic genes, and the oligonucleotide in situ synthesis technology is adopted on a connecting joint of each probe sequence to conduct large-scale synthesisof oligonucleotide on a chip, the oligonucleotide on the chip is eluted with ammonia water to form an oligonucleotide mixture, and a biotin-labeled primer at 5' end is adopted to form a DNA probe library of biotin-labeled obesity-related pathogenic genes in a PCR method. A high-throughput screening method and application for obesity-related known sites are achieved, are faster, more economical and simpler than detection of single site-specific screening and whole-genome or whole-exon sequencing, have low equipment and environmental requirements, can be used in the polymorphic sites of obesity-related known nuclear genes, and is suitable for large-scale screening and preventive examination of obesity-related people.

The invention discloses probe automatic detection equipment, which comprises a base module. The lower end of the base module is provided with a plurality of supporting legs; and the upper end of the base module is provided with a first UR5 robot and a second UR5 robot. The first UR5 robot and the second UR5 robot are provided with a first probe detection mechanism and a second probe detection mechanism respectively. The upper end of the base module is provided with a product jig module. The upper end of the product jig module is provided with a 3D sensor motion module. One side wall of the base module is provided with an operation panel. The invention also provides a detection method of the probe automatic detection equipment. The probe automatic detection equipment is reasonable in structure design, and has the advantages of fast operation, low product defect rate, high uniformity and greatly-reduced missed inspection rate.

The invention provides a gene detection method of Leber's hereditary optic neuropathy, (LHON), a gene chip and a kit. The kit comprises a plurality of PCR probes and primers for detecting SNP of optic nerve lesion genes. The chip is provided with detection probes aiming at 13 mitochondrial genes and 46 SNP loci. According to the gene detection method, the kit and the gene chip are utilized to perform detection, the gene detection method comprises the following steps: performing amplification and hybridization on marked samples; scanning hybridization signals; obtaining chip data, and processing the obtained chip data; judging SNP loci, and judging and reading SNP loci information of the visual lesion genes. The gene detection method disclosed by the invention overcomes the detects that by adopting the direct sequencing, the using sample volume is large and the blood sample quantity is large, the amplification time is long, the amplification frequency is high, and false positive or false negative results can be easily caused, and the like, so that the pain of detected persons can be relieved, and not only be the detection accuracy improved, but also the detection time is shortened. According to the invention, the chip and the kit which have the characteristics of being rapid and being high in flux are provided, so that the early diagnosis and treatment of diseases can be facilitated, and nationwide large-scale screening and preventive inspection are convenient to carry out.

The invention provides a kit for detecting deaf related mitochondrial DNA T7505C mutation. The kit is composed of a DNA extraction mixed liquid, a PCR mixed liquid for T7505C fragment amplification, a pair of outer primers designed against T7505C, a pair of inner primers designed against the T7505C, a restrictive endonuclease, a positive contrast, a negative contrast and a kit body. A protease K digestion cracking method is utilized in the invention to rapidly extract genome DNA from a small amount of specimens, so a problem of the traditional extraction of DNA from the blood of a deaf patient is solved, the pains of a detected person is mitigated, and a trans-regional sample transmission problem is also solved. The kit has the advantages of low application cost, simple and rapid detection flow, visual result interpretation, ensuring of the specificity and the stability of the detection result, and realization of the application in the detection of the deaf related mitochondrial tRNAGlnT7505C mutation.

The invention discloses a mutation detection lit for Parkinson disease and leukoaraiosis and a detection method thereof, which belong to the field of molecular diagnosis. The kit is provided with a packaging box, two pairs of primer sequences, a PCR (polymerase chain reaction) amplification reaction reagent, an enzyme digestion reaction reagent, a positive standard substance and a negative standard substance. The kit is applied in detection for the related mutations rs2066842 and rs75932628 of Parkinson disease and leukoaraiosis. The detection method comprises the following steps: using software Primer 5.0 and software Oligo 7.0 for obtaining specific PCR amplification products containing rs2066842 and rs75932628 according to the sequences of gene CARD15 and gene TREM2 of human respectively; digesting the specific PCR amplification products by restriction enzymes BamHI and HhaI; and detecting whether the two sites are mutated and the mutation types according to the quantity and size of digestion segments and in combination of the capillary electrophoresis.