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39results about How to "Strong detection sensitivity" patented technology
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Photoacoustic spectroscopy telemetering method and device based on micro quartz tuning fork optoacoustic effect
InactiveCN103105365AIncrease luminous energy densityImprove detection sensitivityColor/spectral properties measurementsOptical pathTest object
The invention discloses a photoacoustic spectroscopy telemetering method and device based on a micro quartz tuning fork optoacoustic effect. The method comprises the following steps of: modulating continuous light of which the wavelength changes continuously into light of which the light intensity changes according to a certain frequency, and irradiating the light of which the light intensity changes to an object to be tested; collecting light reflected by the object to be tested by a concave mirror, and irradiating the collected light to a micro quartz tuning fork, wherein the amplitude of the micro quartz tuning fork changes along with the wavelength of incident light waves; and irradiating an arm of the micro quartz tuning fork by another beam of laser, wherein the light spot position of the reflected laser beam moves along with the vibration of the micro quartz tuning fork, the reflected light is irradiated to a light spot position detector after the light path of the reflected light is lengthened through a folded path, the amplitude size of the micro quartz tuning fork can be obtained by detecting the movement of the light spot, and the absorption spectrum of the tested object can be obtained by continuously changing the output wavelength of a variable wavelength laser. The method does not need sample preparation and pre-concentration processes or a photoacoustic cell, and can be used for directly and remotely measuring objects in an open environment.
Owner:XI AN JIAOTONG UNIV
Quantum dot array spectrum sensor
InactiveCN106768331AHigh wavelength resolutionThe number of encoding bits increasesSpectrum investigationPhotovoltaic detectorsLight sensing
The invention discloses a quantum dot array spectrum sensor, comprising: a quantum dot colloidal array film and an array photoelectric detector; the quantum dot colloidal array film is used for bearing a quantum dot colloidal array; the quantum dot colloidal array includes a plurality of quantum dot colloidal units, wherein at least one quantum dot colloidal unit has a different absorbing or transmitting spectrum from other quantum dot colloidal units; the side of the quantum dot colloidal array film bearing the quantum dot colloidal array is opposite to a light-sensing side of the array photoelectric detector; after measured light of measured spectrum irradiates the quantum dot colloidal array film, transmitting light passing through the quantum dot colloidal array is detected by the light-sensing side. With the cooperation of the quantum dot colloidal array and the array photoelectric detector, the quantum dot array spectrum sensor has great bandwidth, high wavelength resolution and good sensitivity to weak light, and properties of existing instruments are improved greatly.
Owner:杭州盗火者科技有限公司
Measurement method and measurement system of zero-field paramagnetic resonance
ActiveCN108333207AStrong detection sensitivityImprove spatial resolutionAnalysis using electron paramagnetic resonanaceZero fieldPhysics
The invention discloses a measurement method and measurement system of zero-field paramagnetic resonance. According to the measurement method, a paramagnetic resonance spectrum of target electrons ismeasured by utilizing an NV color center in a diamond base material as a probe; a zero-field condition is utilized, adverse effects caused by molecule random orientation can be resisted and the Zeemaneffect generated by an external electric field can be eliminated, so that a needed energy grade structure is directly detected. For example, a space structure of a molecule can be obtained through analyzing an electron-electron fine interaction effect; a local polar environment of the molecule can be obtained through analyzing an electron-nuclear spin superfine interaction effect; a precision displacement device, an optical co-focusing device and an NV color center probe are combined, and the precision displacement device and a co-focusing microscopic device can realize accurate finding and detection of the NV color center; the NV color center is a single-electron probe and has a nano-scale resolution capability, so that a nano-scale detection capability is realized.
Owner:UNIV OF SCI & TECH OF CHINA
Preparation method and application of aggregation induced luminescence-molecular imprinting fluorescence sensor for detecting rhodamine B
ActiveCN109406474AHigh fluorescence quantum yieldImprove structural stabilityFluorescence/phosphorescenceWarfarinCross-link
The invention discloses a preparation method and application of aggregation induced luminescence-molecular imprinting fluorescence sensor for detecting rhodamine B. According to the method, the molecular imprinting technology and fluorescence detecting technology are combined, warfarin is used as a template molecule, alpha-methacrylic acid is used as a functional monomer, ethylene glycol dimethylacrylate is used as a cross-linking agent, azobisisobutyronitrile is used as an evocating agent, acetonitrile is used as a dissolvant, functionalized AIE molecules are added, and the precipitation polymerization method is adopted to synthesize AIE-MIPs. The AIE-MIPs is simple to operate, organic reagent use is little, ability to recognize the rhodamine B is high, the linear relationship is good inthe concentration range of 1*10<-5>-10*10<-5>mol / L. The AIEMIPs is adopted to carry out standard recovery experiment for papaya dry and Fanta beverage, the results showthat the recovery range of therhodamine B is 96.2%-103.5%, and the relative standard deviation range is 1.5-4.7%. The data indicates that the AIE-MIPs obtained by the combination of fluorescence detecting and molecular imprintingcan be applied to detection of rhodamine B in practical samples.
Owner:HENAN UNIVERSITY OF TECHNOLOGY
Indirect background fluorescence colloidal gold immunochromatographic test strip based on double-labeled signal amplification and application thereof
The invention discloses an indirect background fluorescence colloidal gold immunochromatographic test strip based on double-labeled signal amplification and application thereof. The test strip comprises five parts, namely a PVC plastic back plate paved with fluorescence, a PVC bottom plate, a nitrocellulose membrane, a sample pad and absorbent paper. The invention also discloses a double-labeled colloidal gold probe, the double-labeled colloidal gold probe is composed of a gold nanoparticle labeled biotinylated anti-target object antibody and gold nanoparticle labeled streptavidin, and signalamplification is realized by using superstrong affinity between biotin-streptavidin molecules. According to the fluorescent colloidal gold immunochromatography test strip disclosed by the invention, asample is pretreated by utilizing the immunomagnetic microspheres before detection, and an enriched target object can be efficiently and rapidly separated in an external magnetic field. The test strip has the advantages of good stability, high sensitivity and no matrix interference, can realize rapid qualitative or quantitative detection of the sample, is simple in operation steps, strong in controllability, and has good application prospects.
Owner:CHINA AGRI UNIV
Fluorescent probe FAL1 for formaldehyde and pH dual-function detection as well as preparation method and application of fluorescent probe FAL1
PendingCN114149369ARaw materials are easy to obtainStrong photochemical stabilityOrganic chemistryFluorescence/phosphorescenceFluoProbesBifunctional
The invention belongs to the field of chemical detection, and relates to a formaldehyde and pH bifunctional detection reagent, in particular to a formaldehyde and pH bifunctional fluorescent molecule based on a naphthalimide derivative as well as a preparation method and application of the formaldehyde and pH bifunctional fluorescent molecule. The recognition performance of the probe FAL1 in various common analytes of DMSO / Tris-HCl is researched through a fluorescence spectrophotometer. Research results show that the probe FAL1 has specific fluorescence recognition performance on formaldehyde, can overcome interference of other common analytes, and has a wide pH value application range. The lowest detection limit of the probe to formaldehyde is 38 nM, and the probe is successfully applied to detection of formaldehyde in tap water through a standard recovery test; the fluorescence emission response of the probe to the pH value is within the pH value range of 3-8, and the probe is suitable for pH marking in a pH-value-sensitive microenvironment in an organism and has high practical application value in the fields of environment detection and biology.
Owner:河南省农业科学院农业质量标准与检测技术研究所 +1
Rs 3909184 detection genotyping kit based on AllGlo probe and genotyping method thereof
InactiveCN105483279AIncrease the Tm valueImprove signal-to-noise ratioMicrobiological testing/measurementPositive controlFluorescence
The invention relates to single nucleotide polymorphism, in particular to an rs 3909184 detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, and rs 3909184 is an SNP (single nucleotide polymorphism) locus serial number provided by NCBI(National Center of Biotechnology Information). The detection genotyping method includes the steps that DNA in an EDTA anticoagulant whole blood sample is extracted by adopting a conventional method; the real-time fluorescence quantification PCR reagent provided by the rs 3909184 detection genotyping kit based on the AllGlo probe is used for conducting real-time fluorescence quantification PCR amplification on DNA; the rs 3909184 SNP locus is genotyped according to detected fluorescence signals. On the premise of keeping high specificity and sensibility of the AllGlo probe, the detection price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
ELISA kit for detecting airborne allergen based on IgG3 antibody
The invention provides an ELISA kit for detecting an airborne allergen based on an IgG3 antibody, and the ELISA kit belongs to the technical field of airborne allergen detection. The ELISA kit includes the following reagents: a detection plate coated with the airborne allergen, a washing buffer solution, a sample diluent, a standard substance or a positive quality control substance, a biotin-labelled anti-human IgG3 antibody, an enzyme-labeled avidin solution, a substrate coloring solution, and a stop solution. The ELISA kit of the invention selects the IgG3 to detect the airborne allergen, and is the prepared ELISA kit based on an indirect method; and the ELISA kit has the characteristics of strong sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at one time. Embodiments of the invention prove that the detection sensitivity of the ELISA kit of the invention reaches 1 ng / ml.
Owner:何韶衡
Novel coronavirus IgG/IgA antibody detection kit
PendingCN111562365ASimple preparation processEasy to useBiological material analysisCoronavirus antibodyGold labelling
The invention discloses a novel coronavirus IgG / IgA antibody detection kit, and belongs to the technical field of biological detection. The kit comprises a colloidal gold detection reagent card and abuffer solution; the colloidal gold detection reagent card comprises a plastic bottom plate, a buffer solution pad is adhered to one end of the plastic bottom plate, one end of the buffer solution padis tightly connected to a gold-labeled pad in a pressing manner, one end of the gold-labeled pad is tightly connected to a sample pad in a pressing manner, one end of the sample pad is tightly connected to a gold-labeled mouse IgG antibody pad in a pressing manner, one end of the gold-labeled mouse IgG antibody pad is tightly connected to a nitrocellulose NC membrane in a pressing manner, the nitrocellulose NC membrane is sequentially coated with a detection line A, a detection line G and a quality control line C which are separated from one another, and the other end of the nitrocellulose NCmembrane is connected to a water absorption pad. The kit is low in cost, convenient to use, high in detection sensitivity, strong in anti-interference capability and short in detection time, and canbe used for clinicians to quickly judge whether the novel coronavirus is diagnosed or not definitely.
Owner:ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
Glu504lys detection genotyping kit based on AllGlo probe and genotyping method thereof
InactiveCN105969842AAccurate typingImprove featuresMicrobiological testing/measurementPositive controlAnti freezing
The invention relates to single nucleotide polymorphism, in particular to a Glu504lys detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, Glu504lys is an SNP locus in an ALDH2 gene of human chromosome 12 provided by NCBI. The genotyping method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the Glu504lys detection genotyping kit based on the probe AllGlo is used for conducting real-time fluorescence quantification PCR amplification on the DNA; and thirdly, genotyping is conducted on the Glu504lys locus of the human gene ALDH2 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
ELISA kit for detecting food allergen based on IgA antibody
The invention provides an ELISA kit for detecting a food allergen based on an IgA antibody, and the kit belongs to the technical field of food allergen detection. The kit includes the following reagents: a detection plate coated with the food allergen, a washing buffer solution, a sample diluent, a standard substance or a positive quality control substance, a biotin-labelled anti-human IgA antibody, an enzyme-labeled avidin solution, a substrate coloring solution, and a stop solution. The ELISA kit of the invention selects the IgA for the first time to detect the food allergen, and is the prepared ELISA kit based on an indirect method; and the ELISA kit has the characteristics of strong sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at onetime. Embodiments of the invention prove that the detection sensitivity of the kit of the invention reaches 10 ng / ml.
Owner:何韶衡
CYP2C19*2 detection parting kit based on probe AllGlo and parting method of CYP2C19*2 detection parting kit
InactiveCN105671151AAccurate typingImprove featuresMicrobiological testing/measurementPositive controlAnti freezing
The invention discloses a CYP2C19*2 detection parting kit based on a probe AllGlo and a parting method of the CYP2C19*2 detection parting kit and relates to single nucleotide polymorphism (SNP). The kit comprises a real-time fluorescence quantification PCR reagent, positive control and negative control. CYP2C19*2 is an SNP locus in a CYP2C19 gene of human chromosome 10 provided by NCBI. The detection parting method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the detection parting kit is used for conducting real-time fluorescence quantification PCR amplification on the DNA; thirdly, parting is conducted on the SNP CYP2C19*2 of the human gene CYP2C19 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
Magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides
ActiveCN110130099AImprove hydrophilicityExcellent strong magnetic response abilityFibre treatmentPreparing sample for investigationThiolNanofiber
The invention provides a magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides. The material is used for glycopeptide enrichment and identification. One-dimensional hydroxyapatite nanofibers (HN) are used as carriers to fix Fe3O4 nanoparticles and Au nanoparticles, and surface modification is performed by the affinity interaction betweenthiol groups in zwitterionic tripeptide L-glutathione and Au and Fe3O4 to form mHN / Au-GSH nanofibers. The mHN / Au-GSH nanofibers have high detection sensitivity (2fmol), high recovery rate (89.65%), strong binding ability (100mg / g), and high enrichment selectivity (1:100) on glycopeptides.
Owner:ZHEJIANG FORESTRY UNIVERSITY
ELISA kit for detecting airborne allergen based on IgM antibody
InactiveCN108593932AStrong detection sensitivityRich varietyBiological material analysisBiological testingIndirect MethodEnzyme
The invention provides an ELISA kit for detecting an airborne allergen based on an IgM antibody, and belongs to the technical field of airborne allergen detection. The kit is prepared from the following reagents: a test board coated with the airborne allergen, a washing buffer, a sample diluent, a standard substance or a positive quality control material, a biotin-labeled anti-human IgM antibody,an enzyme-labeled avidin solution, a color-substrate solution and a stop buffer. According to the ELISA kit provided by the invention, IgM is selected for detecting the airborne allergen; in addition,the ELISA kit is prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can treat large quantities of samples at a time. The embodiment proves that the detection sensitivity of the kit provided by the invention reaches 10ng / ml.
Owner:何韶衡
An oxygen absorption rate measuring device and measuring method
InactiveCN106018306BImprove linearitySimple structureColor/spectral properties measurementsUsing reradiationMeasurement deviceLighting spectrum
Owner:PEOPLES LIBERATION ARMY ORDNANCE ENG COLLEGE
A method for simultaneously measuring the contents of nicotine, 3-(pyrrolidin-2-yl)pyridine, pyridylpyrrolidone and testosterone in serum
ActiveCN110146615BMeet trace analysis needsHigh detection sensitivityComponent separationCotinineFluid phase
Owner:WENZHOU VOCATIONAL COLLEGE OF SCI & TECH
Sensor, preparation method and application thereof, and detection method of 2, 4, 6-trinitrophenol
InactiveCN110687081ASignificant optical signal amplification effectHigh fluorescence intensityFluorescence/phosphorescenceFluorescenceNitrophenol
The invention relates to a sensor, a preparation method and an application thereof, and a detection method of 2, 4, 6-trinitrophenol. The sensor comprises a substrate, a nano metal rod array vertically distributed on the surface of the substrate and molybdenum disulfide quantum dots loaded on nano metal rods, wherein the interval between every two adjacent nano metal bars does not exceed 1 nm, andthe average diameter of the molybdenum disulfide quantum dots is 2-4 nm. The sensor can emit green fluorescence with relatively high intensity, is high in human eye distinguishable degree, has wide universality and high efficiency, is high in fluorescence efficiency, can specifically recognize TNP, obviously quenches a fluorescence signal of to-be-detected TNP after the to-be-detected TNP is captured, is high in detection sensitivity, and can be used for detecting trace TNP.
Owner:北威(重庆)科技股份有限公司
ELISA kit for detecting food allergen based on IgD antibody
InactiveCN108872597AStrong detection sensitivityRich varietyBiological material analysisBiological testingElisa kitBiotin-binding proteins
The invention provides an ELISA kit for detecting a food allergen based on an IgD antibody, which belongs to the technical field of food allergen. The kit comprises the following reagents: a detectionplate coated with food allergen; a washing buffering solution; a sample diluting solution; a standard product or a positive quality control product; a biotin-marked anti-human IgD antibody preparation; an enzyme-labeled avidin solution; a substrate developing solution; and a terminating solution. The IgD is first used for detecting the food allergen; the ELISA kit prepared in an indirect method has the characteristics of high detection sensitivity, high accuracy and good repetition performance; and moreover, a great amount of samples can be treated at one time. Embodiments prove that the detection sensitivity of the kit provided by the invention reaches 2ng / ml.
Owner:何韶衡
Determination method of allylamine and impurities thereof
PendingCN114414697AEffective quality controlStrong detection sensitivityComponent separationPropylaminePhysical chemistry
The invention relates to the technical field of chemical analysis technologies, in particular to a method for determining allylamine and impurities thereof. The method for determining the allylamine and the impurities thereof comprises the step of determining the allylamine and the impurities thereof in an allylamine product by utilizing gas chromatography. Wherein the detection conditions are as follows: a chromatographic column is a 5% diphenyl-95% dimethyl polysiloxane capillary chromatographic column; the temperature programming comprises the following steps: keeping the initial temperature at 30-50 DEG C for 2-5 minutes, raising the temperature to 60-80 DEG C at the speed of 2-20 DEG C / min, keeping the temperature for 1-10 minutes, raising the temperature to 200-250 DEG C at the speed of 2-20 DEG C / min, and keeping the temperature for 1-10 minutes. The method is suitable for separation and detection of the allylamine and the six impurities thereof, separation and content detection of the allylamine and the six impurities thereof can be achieved at the same time, and the separation and detection efficiency is improved.
Owner:湖北华世通生物医药科技有限公司
ELISA kit for detecting airborne allergens based on IgG4 antibody
InactiveCN108646023AStrong detection sensitivityRich varietyBiological testingElisa kitQuality control
The invention provides an ELISA kit for detecting airborne allergens based on an IgG4 antibody, and belongs to the technical field of the detection of airborne allergens. The ELISA kit includes the following reagents: a test plate coated with the airborne allergens; a washing buffer; a sample diluent; a standard or a positive quality control; a biotin-labeled anti-human IgG4 antibody; an enzyme-labeled avidin solution; a substrate color developing solution; and a stop solution. The ELISA kit adopts the IgG4 to detect the airborne allergens, and the ELISA kit is an ELISA kit prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at one time. The embodiment proves that the ELISA kit for detecting the airborne allergens based on the IgG4 antibody has a detection sensitivity of 1 ng / ml.
Owner:何韶衡
A method and system for measuring zero-field paramagnetic resonance
ActiveCN108333207BStrong detection sensitivityImprove spatial resolutionAnalysis using electron paramagnetic resonanaceSingle electronColour centre
Owner:UNIV OF SCI & TECH OF CHINA
ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting airborne allergen based on IgG2 antigen
The invention provides an ELISA (Enzyme Linked Immunosorbent Assay) kit for detecting an airborne allergen based on an IgG2 antigen, and belongs to the field of airborne allergen detection. The kit consists of a detection board coated with the airborne allergen, a washing buffer, a sample diluent, a standard substance or a positive quality control product, a biotin-labeled anti-human IgG2 antibody, an enzyme-labeled avidin solution, a substrate developing solution and a stop solution. IgG2 is used for detecting the airborne allergen, the ELISA kit is prepared based on an indirect method and has the characteristics of high detection sensitivity, high accuracy and high reproducibility, and a large batch of samples can be treated through at one time. As proved by the embodiment, the kit provided by the invention is up to 0.5ng / ml in the detection sensitivity.
Owner:JINZHOU MEDICAL UNIV
VKORC1 gene polymorphism detection genotyping kit based on AllGlo probe and genotyping method thereof
InactiveCN105483280ALow costAccurate typingMicrobiological testing/measurementVKORC1Positive control
The invention relates to single nucleotide polymorphism, in particular to a VKORC1 gene polymorphism detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, and VKORC1(1173C>T) is the SNP (single nucleotide polymorphism) locus, provided by NCBI(National Center of Biotechnology Information), of human chromosome 16 in the VKORC1 gene. The genotyping method includes the steps that DNA in an EDTA anticoagulant whole blood sample is extracted by adopting a conventional method; the real-time fluorescence quantification PCR reagent provided by the VKORC1 gene polymorphism detection genotyping kit based on the AllGlo probe is used for conducting real-time fluorescence quantification PCR amplification on DNA; the SNP locus VKORC1(1173C>T) of the human VKORC1 gene is genotyped according to detected fluorescence signals.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
Biological fluorescent probe for dynamically monitoring cholecystokinin and application thereof
PendingCN113234691AHigh fluorescence intensityDecreased fluorescence intensityFluorescence/phosphorescenceFermentationSynapseBiophysics
The invention provides a biological fluorescent probe for dynamically monitoring cholecystokinin and application thereof, and relates to the technical field of biological medicines. The biological fluorescent probe (CCK probe) is provided with an element capable of specifically recognizing neurons carrying Cre-protease, so that the biological fluorescent probe can be expressed in the specific neurons; the CCK probe can explore the release rule of the neuronal synapse CCK neurotransmitter by comparing the change of the fluorescence intensity of the biological fluorescent probe. The CCK probe can mark the cholecystokinin in specific neurons and dynamically detect the release process of the cholecystokinin after neuronal synapse, can specifically recognize the cholecystokinin before and after the synapse, and is high in detection sensitivity, strong in anti-interference capability and low in trauma.
Owner:黄燕婷
Magnetic nanofiber-based zwitterionic hydrophilic materials for selective capture and recognition of glycopeptides
ActiveCN110130099BImprove hydrophilicityExcellent strong magnetic response abilityFibre treatmentPreparing sample for investigationPolymer scienceNanoparticle
The present invention proposes a magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides for glycopeptide enrichment and identification; utilizing one-dimensional hydroxyapatite nanofibers (HN) as a carrier to fix Fe 3 o 4 Nanoparticles and Au nanoparticles, through the thiol group in the zwitterionic tripeptide L-glutathione and Au and Fe 3 o 4 Surface modification by the affinity between them to form mHN / Au‑GSH nanofibers. mHN / Au‑GSH nanofibers have high detection sensitivity (2fmol), high recovery (89.65%), strong binding capacity (100mg / g) and high enrichment selectivity (1:100) for glycopeptides.
Owner:ZHEJIANG FORESTRY UNIVERSITY
VKORC1 gene polymorphism detecting and genotyping kit based on AllGlo probe and genotyping method thereof
InactiveCN105671152AIncrease the Tm valueImprove signal-to-noise ratioMicrobiological testing/measurementPositive controlFluorescence
The invention discloses a VKORC1 gene polymorphism detecting and genotyping kit based on an AllGlo probe and a genotyping method thereof and relates to single nucleotide polymorphisms. The kit comprises a real-time fluorescent quantitative PCR reagent, positive control and negative control. VKORC1(-1639A / G) provides an SNP locus in a VKORC1 gene of a chromosome 16 of a person for NCBI. The genotyping method includes the steps that firstly, DNA in an EDTA anticoagulant whole-blood sample is extracted through a conventional method; secondly, the real-time fluorescent quantitative PCR reagent provided by the detecting and genotyping kit performs real-time fluorescent quantitative PCR amplification on the DNA; thirdly, genotyping is performed on the single nucleotide polymorphisms locus VKORC1(-1639A / G) of the VKORC1 gene of a person according to a detected fluorescence signal. On the premise that high specificity and high sensitivity of the AllGlo probe are kept, the detection price is lower than that of a direction sequencing method, the process is simpler, and consumed time is shorter.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
ELISA kit for detecting airborne allergen based on IgA antibody
InactiveCN108593934AStrong detection sensitivityImprove accuracyBiological material analysisBiological testingIndirect MethodEnzyme
The invention provides an ELISA kit for detecting an airborne allergen based on an IgA antibody, and belongs to the technical field of airborne allergen detection. The kit is prepared from the following reagents: a test board coated with the airborne allergen, a washing buffer, a sample diluent, a standard substance or a positive quality control material, a biotin-labeled anti-human IgA antibody,an enzyme-labeled avidin solution, a color-substrate solution and a stop buffer. According to the ELISA kit provided by the invention, IgA is selected for detecting the airborne allergen; in addition,the ELISA kit is prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can treat large quantities of samples at a time. The embodiment proves that the detection sensitivity of the kit provided by the invention reaches 10ng / ml.
Owner:何韶衡
Surface Raman enhanced optical fiber probe and preparation method thereof
PendingCN112986207AStrong detection sensitivityGood repeatabilityRaman scatteringMaterials scienceAnalytical chemistry
The invention discloses a preparation method of a surface Raman enhanced optical fiber probe. The preparation method comprises the following steps: preparing a seed solution; adding the seed solution into a HAuCl4 solution to prepare a gold nanostar solution; processing the end surface of the optical fiber; and covering the gold nanostars on the surface of the end face of the processed optical fiber.
Owner:杭州苏铂科技有限公司
ELISA kit for detecting food allergen based on IgG2 antibody
InactiveCN108802359AStrong detection sensitivityRich varietyBiological testingElisa kitQuality control
The invention provides an ELISA kit for detecting food allergen based on IgG2 antibody, and belongs to the technical field of food allergen. The kit is prepared from the following reagents: a detection plate coated with food allergen; a wash buffer solution, a sample diluent, a standard or a positive quality control, a biotin labeled anti-human IgG2 antibody preparation, an enzyme labeled avidin solution, a substrate developing solution and a stop solution. According to the ELISA kit provided by the invention, IgG2 is adopted to detect food allergen for the first time; the ELISA kit prepared based on an indirect method has the characteristics of high detection sensitivity, high accuracy and good reproducibility, and can be used for treating a large batch of samples once. Embodiments provethat the detection sensitivity of the kit provided by the invention can reach 0.5ng / ml.
Owner:何韶衡
CYP2C9*3 detection parting kit based on probe AllGlo and parting method of CYP2C9*3 detection parting kit
InactiveCN105671153AIncrease the Tm valueImprove signal-to-noise ratioMicrobiological testing/measurementPositive controlAnti freezing
The invention discloses a CYP2C9*3 detection parting kit based on a probe AllGlo and a parting method of the CYP2C9*3 detection parting kit and relates to single nucleotide polymorphism (SNP). The kit comprises a real-time fluorescence quantification PCR reagent, positive control and negative control. CYP2C9*3 is an SNP locus in a CYP2C9 gene of human chromosome 10 provided by NCBI. The parting method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the CYP2C9*3 detection parting kit based on the probe AllGlo is used for conducting real-time fluorescence quantification PCR amplification on the DNA; thirdly, parting is conducted on the CYP2C9*3 locus of the human gene CYP2C9 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.
Owner:ZHONGSHAN HOSPITAL XIAMEN UNIV +1
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