4results about How to "Strong detection sensitivity" patented technology

The application relates to a gold nanoparticle array preparation method with strong stability and reusability, high cysteamine-stabilized gold nanoparticles are prepared first, the spacing between the gold nanoparticles is changed by utilizing the coulomb repulsion between the gold nanoparticles, then the gold nanoparticle solution is added dropwise to a glass surface modified by gamma-glycidoxypropyltrimethoxysilane to react, stable covalent bonds are formed, and gold nanoparticle arrays with uniform distribution on the glass surface can be obtained after the reaction. The preparation does not require high temperature, the experimental operation is simple and convenient, the gold nanoparticles are arranged in order and closely, have high detection sensitivity, have strong ultraviolet absorption capacity, good stability, reusability, salt resistance and low background interference, and can be reused. The gold nanoparticle array can realize detection analysis on high-salt-content samples in surface-assisted laser desorption ionization mass spectrometry and be used for rapid analysis of small molecule compounds in positive and negative ion dual modes.

The invention belongs to the field of chemical detection, and discloses a fluorescent probe P1, a preparation method thereof and application of the fluorescent probe P1 in spermidine and viscosity dual-function detection. The structural formula of the fluorescent probe P1 is shown in the specification. The recognition performance of the probe P1 in various common analytes in a DMF-HEPES buffer system is researched through a fluorescence spectrophotometer. Results show that the probe P1 has specific fluorescence recognition performance on spermidine, can overcome interference of other common analytes, and has a wide pH value application range. The probe has relatively good sensitivity on the recognition of spermidine, and the lowest detection limit is 0.213 mu M. In addition, the probe P1 has a good response effect on the viscosity of the solution, is not interfered by the polarity of the solution, and can realize quantitative detection of the viscosity. The results show that the probe P1 has wide application prospects in the fields of food safety, environmental detection and biology.

The invention discloses a fenaminosulf hapten, an artificial antigen, an antibody and a preparation method and application thereof. According to the fenaminosulf hapten disclosed by the invention, a carboxyl active group with a spacer arm with a certain length is introduced to a sulfocarboxyl group of fenaminosulf, so that the fenaminosulf hapten can be coupled with a carrier protein to obtain an artificial antigen for immunization; the fenaminosulf hapten retains all characteristic groups of fenaminosulf, the original structural characteristics of the fenaminosulf are changed to the minimum extent, and a foundation is laid for generating an antibody with higher specificity and higher sensitivity for subsequently stimulating animal immune response. The fenaminosulf hapten is used for preparing an artificial antigen, and a monoclonal antibody with high detection sensitivity and strong specificity is prepared from the fenaminosulf hapten. According to indirect competitive ELISA based on the fenaminosulf monoclonal antibody, the lowest detection limit of fenaminosulf is 2.45 ng / mL, IC50 is 14.80 ng / mL, the linear range is 4.75-46.06 ng / mL, the detection sensitivity is high, and the linear range is wide.

The application belongs to the technical field of targeted adenovirus, and particularly relates to a nanobody specifically combined with adenovirus Fiber protein and application thereof, and the nanobody 252A7, an amino acid sequence of which is shown as SEQ ID NO. 2. The nanobody 252A7 provided by the application can specifically recognize and combine with Fiber proteins of various serotypes of adenovirus (such as HAdV-3, HAdV-4, HAdV-7, HAdV-55), solves the technical bottleneck that traditional antibodies are difficult to recognize the conserved epitope of Fiber protein and lack broad spectrum, can effectively distinguish adenovirus from other common respiratory viruses (such as RSV, SARS-CoV-2), avoids cross reaction, and improves the accuracy and reliability of clinical etiology diagnosis. The nanobody of the application can be used for developing high-sensitivity immunological detection reagents (such as ELISA, immunochromatography, chemiluminescence, etc.), and has potential application value in the fields of targeted therapy and molecular imaging.