39results about How to "Strong detection sensitivity" patented technology

The invention discloses a photoacoustic spectroscopy telemetering method and device based on a micro quartz tuning fork optoacoustic effect. The method comprises the following steps of: modulating continuous light of which the wavelength changes continuously into light of which the light intensity changes according to a certain frequency, and irradiating the light of which the light intensity changes to an object to be tested; collecting light reflected by the object to be tested by a concave mirror, and irradiating the collected light to a micro quartz tuning fork, wherein the amplitude of the micro quartz tuning fork changes along with the wavelength of incident light waves; and irradiating an arm of the micro quartz tuning fork by another beam of laser, wherein the light spot position of the reflected laser beam moves along with the vibration of the micro quartz tuning fork, the reflected light is irradiated to a light spot position detector after the light path of the reflected light is lengthened through a folded path, the amplitude size of the micro quartz tuning fork can be obtained by detecting the movement of the light spot, and the absorption spectrum of the tested object can be obtained by continuously changing the output wavelength of a variable wavelength laser. The method does not need sample preparation and pre-concentration processes or a photoacoustic cell, and can be used for directly and remotely measuring objects in an open environment.

The invention discloses a measurement method and measurement system of zero-field paramagnetic resonance. According to the measurement method, a paramagnetic resonance spectrum of target electrons ismeasured by utilizing an NV color center in a diamond base material as a probe; a zero-field condition is utilized, adverse effects caused by molecule random orientation can be resisted and the Zeemaneffect generated by an external electric field can be eliminated, so that a needed energy grade structure is directly detected. For example, a space structure of a molecule can be obtained through analyzing an electron-electron fine interaction effect; a local polar environment of the molecule can be obtained through analyzing an electron-nuclear spin superfine interaction effect; a precision displacement device, an optical co-focusing device and an NV color center probe are combined, and the precision displacement device and a co-focusing microscopic device can realize accurate finding and detection of the NV color center; the NV color center is a single-electron probe and has a nano-scale resolution capability, so that a nano-scale detection capability is realized.

The invention discloses a preparation method and application of aggregation induced luminescence-molecular imprinting fluorescence sensor for detecting rhodamine B. According to the method, the molecular imprinting technology and fluorescence detecting technology are combined, warfarin is used as a template molecule, alpha-methacrylic acid is used as a functional monomer, ethylene glycol dimethylacrylate is used as a cross-linking agent, azobisisobutyronitrile is used as an evocating agent, acetonitrile is used as a dissolvant, functionalized AIE molecules are added, and the precipitation polymerization method is adopted to synthesize AIE-MIPs. The AIE-MIPs is simple to operate, organic reagent use is little, ability to recognize the rhodamine B is high, the linear relationship is good inthe concentration range of 1*10<-5>-10*10<-5>mol/L. The AIEMIPs is adopted to carry out standard recovery experiment for papaya dry and Fanta beverage, the results showthat the recovery range of therhodamine B is 96.2%-103.5%, and the relative standard deviation range is 1.5-4.7%. The data indicates that the AIE-MIPs obtained by the combination of fluorescence detecting and molecular imprintingcan be applied to detection of rhodamine B in practical samples.

The invention relates to single nucleotide polymorphism, in particular to an rs 3909184 detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, and rs 3909184 is an SNP (single nucleotide polymorphism) locus serial number provided by NCBI(National Center of Biotechnology Information). The detection genotyping method includes the steps that DNA in an EDTA anticoagulant whole blood sample is extracted by adopting a conventional method; the real-time fluorescence quantification PCR reagent provided by the rs 3909184 detection genotyping kit based on the AllGlo probe is used for conducting real-time fluorescence quantification PCR amplification on DNA; the rs 3909184 SNP locus is genotyped according to detected fluorescence signals. On the premise of keeping high specificity and sensibility of the AllGlo probe, the detection price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.

The invention provides an ELISA kit for detecting an airborne allergen based on an IgG3 antibody, and the ELISA kit belongs to the technical field of airborne allergen detection. The ELISA kit includes the following reagents: a detection plate coated with the airborne allergen, a washing buffer solution, a sample diluent, a standard substance or a positive quality control substance, a biotin-labelled anti-human IgG3 antibody, an enzyme-labeled avidin solution, a substrate coloring solution, and a stop solution. The ELISA kit of the invention selects the IgG3 to detect the airborne allergen, and is the prepared ELISA kit based on an indirect method; and the ELISA kit has the characteristics of strong sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at one time. Embodiments of the invention prove that the detection sensitivity of the ELISA kit of the invention reaches 1 ng/ml.

The invention discloses a novel coronavirus IgG/IgA antibody detection kit, and belongs to the technical field of biological detection. The kit comprises a colloidal gold detection reagent card and abuffer solution; the colloidal gold detection reagent card comprises a plastic bottom plate, a buffer solution pad is adhered to one end of the plastic bottom plate, one end of the buffer solution padis tightly connected to a gold-labeled pad in a pressing manner, one end of the gold-labeled pad is tightly connected to a sample pad in a pressing manner, one end of the sample pad is tightly connected to a gold-labeled mouse IgG antibody pad in a pressing manner, one end of the gold-labeled mouse IgG antibody pad is tightly connected to a nitrocellulose NC membrane in a pressing manner, the nitrocellulose NC membrane is sequentially coated with a detection line A, a detection line G and a quality control line C which are separated from one another, and the other end of the nitrocellulose NCmembrane is connected to a water absorption pad. The kit is low in cost, convenient to use, high in detection sensitivity, strong in anti-interference capability and short in detection time, and canbe used for clinicians to quickly judge whether the novel coronavirus is diagnosed or not definitely.

The invention provides a magnetic nanofiber-based zwitterionic hydrophilic material for selective capture and recognition of glycopeptides. The material is used for glycopeptide enrichment and identification. One-dimensional hydroxyapatite nanofibers (HN) are used as carriers to fix Fe3O4 nanoparticles and Au nanoparticles, and surface modification is performed by the affinity interaction betweenthiol groups in zwitterionic tripeptide L-glutathione and Au and Fe3O4 to form mHN/Au-GSH nanofibers. The mHN/Au-GSH nanofibers have high detection sensitivity (2fmol), high recovery rate (89.65%), strong binding ability (100mg/g), and high enrichment selectivity (1:100) on glycopeptides.

The invention provides an ELISA kit for detecting an airborne allergen based on an IgM antibody, and belongs to the technical field of airborne allergen detection. The kit is prepared from the following reagents: a test board coated with the airborne allergen, a washing buffer, a sample diluent, a standard substance or a positive quality control material, a biotin-labeled anti-human IgM antibody,an enzyme-labeled avidin solution, a color-substrate solution and a stop buffer. According to the ELISA kit provided by the invention, IgM is selected for detecting the airborne allergen; in addition,the ELISA kit is prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can treat large quantities of samples at a time. The embodiment proves that the detection sensitivity of the kit provided by the invention reaches 10ng/ml.

The invention relates to a sensor, a preparation method and an application thereof, and a detection method of 2, 4, 6-trinitrophenol. The sensor comprises a substrate, a nano metal rod array vertically distributed on the surface of the substrate and molybdenum disulfide quantum dots loaded on nano metal rods, wherein the interval between every two adjacent nano metal bars does not exceed 1 nm, andthe average diameter of the molybdenum disulfide quantum dots is 2-4 nm. The sensor can emit green fluorescence with relatively high intensity, is high in human eye distinguishable degree, has wide universality and high efficiency, is high in fluorescence efficiency, can specifically recognize TNP, obviously quenches a fluorescence signal of to-be-detected TNP after the to-be-detected TNP is captured, is high in detection sensitivity, and can be used for detecting trace TNP.

The invention provides an ELISA kit for detecting a food allergen based on an IgD antibody, which belongs to the technical field of food allergen. The kit comprises the following reagents: a detectionplate coated with food allergen; a washing buffering solution; a sample diluting solution; a standard product or a positive quality control product; a biotin-marked anti-human IgD antibody preparation; an enzyme-labeled avidin solution; a substrate developing solution; and a terminating solution. The IgD is first used for detecting the food allergen; the ELISA kit prepared in an indirect method has the characteristics of high detection sensitivity, high accuracy and good repetition performance; and moreover, a great amount of samples can be treated at one time. Embodiments prove that the detection sensitivity of the kit provided by the invention reaches 2ng/ml.

The invention provides an ELISA kit for detecting airborne allergens based on an IgG4 antibody, and belongs to the technical field of the detection of airborne allergens. The ELISA kit includes the following reagents: a test plate coated with the airborne allergens; a washing buffer; a sample diluent; a standard or a positive quality control; a biotin-labeled anti-human IgG4 antibody; an enzyme-labeled avidin solution; a substrate color developing solution; and a stop solution. The ELISA kit adopts the IgG4 to detect the airborne allergens, and the ELISA kit is an ELISA kit prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can process large quantities of samples at one time. The embodiment proves that the ELISA kit for detecting the airborne allergens based on the IgG4 antibody has a detection sensitivity of 1 ng/ml.

The invention relates to single nucleotide polymorphism, in particular to a VKORC1 gene polymorphism detection genotyping kit based on an AllGlo probe and a genotyping method thereof. The kit comprises a real-time fluorescence quantification PCR (polymerase chain reaction) reagent, positive control and negative control, and VKORC1(1173C>T) is the SNP (single nucleotide polymorphism) locus, provided by NCBI(National Center of Biotechnology Information), of human chromosome 16 in the VKORC1 gene. The genotyping method includes the steps that DNA in an EDTA anticoagulant whole blood sample is extracted by adopting a conventional method; the real-time fluorescence quantification PCR reagent provided by the VKORC1 gene polymorphism detection genotyping kit based on the AllGlo probe is used for conducting real-time fluorescence quantification PCR amplification on DNA; the SNP locus VKORC1(1173C>T) of the human VKORC1 gene is genotyped according to detected fluorescence signals.

The invention provides a biological fluorescent probe for dynamically monitoring cholecystokinin and application thereof, and relates to the technical field of biological medicines. The biological fluorescent probe (CCK probe) is provided with an element capable of specifically recognizing neurons carrying Cre-protease, so that the biological fluorescent probe can be expressed in the specific neurons; the CCK probe can explore the release rule of the neuronal synapse CCK neurotransmitter by comparing the change of the fluorescence intensity of the biological fluorescent probe. The CCK probe can mark the cholecystokinin in specific neurons and dynamically detect the release process of the cholecystokinin after neuronal synapse, can specifically recognize the cholecystokinin before and after the synapse, and is high in detection sensitivity, strong in anti-interference capability and low in trauma.

The invention discloses a VKORC1 gene polymorphism detecting and genotyping kit based on an AllGlo probe and a genotyping method thereof and relates to single nucleotide polymorphisms. The kit comprises a real-time fluorescent quantitative PCR reagent, positive control and negative control. VKORC1(-1639A/G) provides an SNP locus in a VKORC1 gene of a chromosome 16 of a person for NCBI. The genotyping method includes the steps that firstly, DNA in an EDTA anticoagulant whole-blood sample is extracted through a conventional method; secondly, the real-time fluorescent quantitative PCR reagent provided by the detecting and genotyping kit performs real-time fluorescent quantitative PCR amplification on the DNA; thirdly, genotyping is performed on the single nucleotide polymorphisms locus VKORC1(-1639A/G) of the VKORC1 gene of a person according to a detected fluorescence signal. On the premise that high specificity and high sensitivity of the AllGlo probe are kept, the detection price is lower than that of a direction sequencing method, the process is simpler, and consumed time is shorter.

The invention provides an ELISA kit for detecting an airborne allergen based on an IgA antibody, and belongs to the technical field of airborne allergen detection. The kit is prepared from the following reagents: a test board coated with the airborne allergen, a washing buffer, a sample diluent, a standard substance or a positive quality control material, a biotin-labeled anti-human IgA antibody,an enzyme-labeled avidin solution, a color-substrate solution and a stop buffer. According to the ELISA kit provided by the invention, IgA is selected for detecting the airborne allergen; in addition,the ELISA kit is prepared based on an indirect method, has the characteristics of high sensitivity, high accuracy and good reproducibility, and can treat large quantities of samples at a time. The embodiment proves that the detection sensitivity of the kit provided by the invention reaches 10ng/ml.

The invention discloses a preparation method of a surface Raman enhanced optical fiber probe. The preparation method comprises the following steps: preparing a seed solution; adding the seed solution into a HAuCl4 solution to prepare a gold nanostar solution; processing the end surface of the optical fiber; and covering the gold nanostars on the surface of the end face of the processed optical fiber.

The invention discloses a CYP2C9*3 detection parting kit based on a probe AllGlo and a parting method of the CYP2C9*3 detection parting kit and relates to single nucleotide polymorphism (SNP). The kit comprises a real-time fluorescence quantification PCR reagent, positive control and negative control. CYP2C9*3 is an SNP locus in a CYP2C9 gene of human chromosome 10 provided by NCBI. The parting method comprises the steps that firstly, DNA in an EDTA anti-freezing whole blood sample is extracted through a conventional method; secondly, the real-time fluorescence quantification PCR reagent provided by the CYP2C9*3 detection parting kit based on the probe AllGlo is used for conducting real-time fluorescence quantification PCR amplification on the DNA; thirdly, parting is conducted on the CYP2C9*3 locus of the human gene CYP2C9 according to detected fluorescence signals. On the premise of keeping high specificity and sensitivity of the probe AllGlo, the detecting price is lower than that of a direct sequencing method, the process is simpler, and consumed time is shorter.