COMPOSITIONS AND METHODS FOR THE PURIFICATION OF CAPSID VARIANTS OF THE ADENO-ASSOCIATED VIRUS

Claims

Herein, compositions and methods for the differential separation of adeno-associated and recombinant viral capsid variants are described and provided, wherein the methods comprise at least two chromatographic polishing steps, e.g., cation exchange chromatography steps, anion exchange chromatography steps, hydrophobic interaction chromatography steps and / or mixed-mode chromatography steps.

1. A method for differentially separating capsid variants of recombinant adeno-associated virus (rAAV) vector, said method comprising: (a) contacting a sample comprising rAAV vector particles with an AAV affinity chromatography composition to produce a capture step eluate comprising rAAV vector particles; (b) contacting said capture step eluate with a first polishing chromatography composition to produce a first polishing eluate comprising rAAV vector particles; and (c) contacting said first polishing eluate with a second polishing chromatography composition to produce a second polishing eluate comprising differentially separated rAAV vector capsid variants.