Device and method for detecting biomacromolecules by capillary electrophoresis

Abstract

The invention provides a method and device for detecting biomacromolecules by capillary electrophoresis, and belongs to the technical field of biochemical equipment. The method comprises the following steps of pumping a fresh separation glue solution into a capillary by using an automatic high-pressure pump machine, placing the separation glue solution in the capillary in treated macromolecular biosample sample, to be separated, on a sample tray (96-pore) for electrophoresis sample loading, allowing the macromolecular biosample to be analyzed to be at one end of the capillary, connecting the two ends of the capillary to a high-voltage power supply to separate the biomacromolecules to be separated in the capillary, performing back-and-forth scanning detection by using the automatic macromolecule detection device mounted at one end of the capillary after separation, stopping electrophoresis after the biomacromolecules are detected, transmitting a signal to a processor, and analyzing the components and content of the macromolecular biosample in the capillary by using a system. The device is mounted at the end of the capillary, and is used for continuously detecting biomacromolecules on line; electrophoresis is stopped after biomacromolecules are detected, and data is transmitted to the system.

Description

technical field

[0001] The utility model relates to a device for detecting biological macromolecules by using capillary electrophoresis technology, which belongs to the technical field of biochemical equipment. Background technique

[0002] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the electrophoresis phenomenon, but it was not until Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins in 1937 that the electrophoresis technology began to be applied. In the 1960s and 1970s, when media such as filter paper and polyacrylamide gel were successively introduced into electrophoresis, electrophoresis technology developed rapidly. The rich and colorful electrophoresis forms make it widely used. In addition to the separation and analysis of small molecular substances, it ...

Examples

Embodiment 1

[0024] The above-mentioned device can be as figure 1 and figure 2 In this way, it is installed into an all-in-one machine, including the upper cover 1, the pump status observation window 2, the sample tray inlet 3 and other components;

[0025] The internal structure of the above-mentioned device is as figure 2 As shown, it is composed of a high-pressure pump motor 4, a high-pressure pump 5, an optical scanning part 6, an optical scanning part driving motor 7, a carriage driving motor 8, a capillary sampling end 9, a macromolecule biological sample tray (96 holes) 10, and a capillary tube 11, a water tank 12, a waste liquid tank 13, and a buffer tank 14 are composed.

[0026] At the bottom of the main machine is a frame, on which a movable pallet is arranged, and the pallet is connected with a driving motor 8, and the driving motor can make the pallet move back and forth on the frame, and a buffer is sequentially arranged on the top of the pallet. Tank 14, waste liquid t...

technical field

[0001] The utility model relates to a device for detecting biological macromolecules by using capillary electrophoresis technology, which belongs to the technical field of biochemical equipment. Background technique

[0002] In a DC electric field, the phenomenon that charged particles move to electrodes with opposite signs is called electrophoresis (electrophoresis). In 1809, the Russian physicist Peнce first discovered the electrophoresis phenomenon, but it was not until Tiselius in Sweden established the boundary electrophoresis (boundary electrophoresis) for separating proteins in 1937 that the electrophoresis technology began to be applied. In the 1960s and 1970s, when media such as filter paper and polyacrylamide gel were successively introduced into electrophoresis, electrophoresis technology developed rapidly. The rich and colorful electrophoresis forms make it widely used. In addition to the separation and analysis of small molecular substances, it ...

Examples

Embodiment 1

[0024] The above-mentioned device can be as figure 1 and figure 2 In this way, it is installed into an all-in-one machine, including the upper cover 1, the pump status observation window 2, the sample tray inlet 3 and other components;

[0025] The internal structure of the above-mentioned device is as figure 2 As shown, it is composed of a high-pressure pump motor 4, a high-pressure pump 5, an optical scanning part 6, an optical scanning part driving motor 7, a carriage driving motor 8, a capillary sampling end 9, a macromolecule biological sample tray (96 holes) 10, and a capillary tube 11, a water tank 12, a waste liquid tank 13, and a buffer tank 14 are composed.

[0026] At the bottom of the main machine is a frame, on which a movable pallet is arranged, and the pallet is connected with a driving motor 8, and the driving motor can make the pallet move back and forth on the frame, and a buffer is sequentially arranged on the top of the pallet. Tank 14, waste liquid t...

Images