Method for purifying resting eggs of seawater branchionus plicatilis

Abstract

The invention provides a method for purifying resting eggs of seawater branchionus plicatilis. The method comprises the steps of feeding fresh water into a container, and then feeding a sodium hypochlorite disinfectant to enable available chlorine in the solution to reach 10-20PPm; putting the resting egg raw material of the seawater branchionus plicatilis into the container, mixing, and enabling the resting eggs of the branchionus plicatilis to be fully hydrated; stirring light impurities floating on the upper layer water surface, and then standing still for 30-60 minutes; transferring lower-layer heavy impurities, containing the resting eggs of the branchionus plicatilis and silt, in a mesh bag into a container, wherein the container is filled with high-concentration brine, and the volume of the brine is 10-15 times that of the transferred material; stirring the resting eggs of the branchionus plicatilis floating on the upper layer water surface to enable the silt heavy impurity mixed in the resting eggs of the branchionus plicatilis to be fully separated, and standing still; removing the heavy impurities at the lower layer under the resting eggs of the branchionus plicatilis at the upper layer, and using the mesh bag to receive the resting eggs of the branchionus plicatilis at the upper layer. After the method is adopted, the purification rate of the resting eggs of the seawater branchionus plicatilis reaches up to more than 98%.

Description

technical field

[0001] The invention relates to the technical field of aquaculture. In particular, it relates to a method for purifying resting eggs of Brachionus plicata in seawater. Background technique

[0002] With the rapid development of the aquaculture industry, seawater rotifers have become an indispensable fresh and live bait for aquaculture seedlings. At present, the domestic aquaculture industry mainly uses wild rotifers and dormant eggs of seawater rotifers as provenance. After hatching, artificial cultivation is carried out to provide aquatic seedlings as opening bait. In my country, especially in northern regions, due to the influence of temperature, only in summer and autumn there are wild rotifers, and the quality and supply of harvested rotifers are unstable, which affects the survival rate of seedlings. However, using seawater rotifer dormant eggs as the provenance, after hatching, and then artificially cultivating, most users use the harvested rotifer do...

Examples

Embodiment 1

[0019] The processing and disinfection of embodiment 1 light impurities

[0020] Add fresh water into a transparent conical container, the volume of which is 10 to 15 times the raw material of Brachionus rugosa dormant eggs in seawater, and then add sodium hypochlorite disinfectant to make the available chlorine concentration of the solution 5, 10, 15, 20 , 25PPm. The same batch of harvested seawater dormant rotifer rotifers raw materials were placed in conical containers with different concentrations of available chlorine and stirred to fully hydrate the rotifer dormant eggs. After stratification, slowly stir the light impurities floating on the upper water surface, so that the rotifer dormant eggs mixed in the light impurities can be fully separated, and the yield of rotifer dormant eggs can be increased. Then stand still for 30-60 minutes, the lower layer contains heavy impurities such as rotifer dormant eggs and silt, and the upper layer contains light impurities such as ...

Embodiment 2

[0025] The processing of embodiment 2 heavy impurities

[0026] After draining the heavy impurities such as rotifer dormant eggs and silt received in the net bag, they are transferred to conical containers filled with salinity 15%, 20%, 25% and saturated brine respectively. The brine volume is 10 to 15 times the volume of the material, and stirred to fully dehydrate the dormant eggs of rotifers. Stop stirring, and after stratification, slowly stir the rotifer dormant eggs floating on the upper water surface, so that heavy impurities such as silt mixed in the rotifer dormant eggs can be fully separated, and the purification rate of rotifer dormant eggs can be improved. Then stand still for 30 to 60 minutes, and observe the layering status. In the container filled with brine with a salinity of 15%, after standing still for 60 minutes, some dormant eggs are still suspended in the brine, which shows that the dormant eggs are not completely dehydrated in the brine with a salinity ...

technical field

[0001] The invention relates to the technical field of aquaculture. In particular, it relates to a method for purifying resting eggs of Brachionus plicata in seawater. Background technique

[0002] With the rapid development of the aquaculture industry, seawater rotifers have become an indispensable fresh and live bait for aquaculture seedlings. At present, the domestic aquaculture industry mainly uses wild rotifers and dormant eggs of seawater rotifers as provenance. After hatching, artificial cultivation is carried out to provide aquatic seedlings as opening bait. In my country, especially in northern regions, due to the influence of temperature, only in summer and autumn there are wild rotifers, and the quality and supply of harvested rotifers are unstable, which affects the survival rate of seedlings. However, using seawater rotifer dormant eggs as the provenance, after hatching, and then artificially cultivating, most users use the harvested rotifer do...

Examples

Embodiment 1

[0019] The processing and disinfection of embodiment 1 light impurities

[0020] Add fresh water into a transparent conical container, the volume of which is 10 to 15 times the raw material of Brachionus rugosa dormant eggs in seawater, and then add sodium hypochlorite disinfectant to make the available chlorine concentration of the solution 5, 10, 15, 20 , 25PPm. The same batch of harvested seawater dormant rotifer rotifers raw materials were placed in conical containers with different concentrations of available chlorine and stirred to fully hydrate the rotifer dormant eggs. After stratification, slowly stir the light impurities floating on the upper water surface, so that the rotifer dormant eggs mixed in the light impurities can be fully separated, and the yield of rotifer dormant eggs can be increased. Then stand still for 30-60 minutes, the lower layer contains heavy impurities such as rotifer dormant eggs and silt, and the upper layer contains light impurities such as ...

Embodiment 2

[0025] The processing of embodiment 2 heavy impurities

[0026] After draining the heavy impurities such as rotifer dormant eggs and silt received in the net bag, they are transferred to conical containers filled with salinity 15%, 20%, 25% and saturated brine respectively. The brine volume is 10 to 15 times the volume of the material, and stirred to fully dehydrate the dormant eggs of rotifers. Stop stirring, and after stratification, slowly stir the rotifer dormant eggs floating on the upper water surface, so that heavy impurities such as silt mixed in the rotifer dormant eggs can be fully separated, and the purification rate of rotifer dormant eggs can be improved. Then stand still for 30 to 60 minutes, and observe the layering status. In the container filled with brine with a salinity of 15%, after standing still for 60 minutes, some dormant eggs are still suspended in the brine, which shows that the dormant eggs are not completely dehydrated in the brine with a salinity ...