Method for preparing a monolithic support immobilized thereon with uranyl cations, and related methods for capture and recovery

By preparing a whole carrier in the channel of the miniaturized analysis system, using a polymerization solution of phosphate groups, crosslinking agents and free radical polymerization initiator, UO22+ cations are synthesized and immobilized in situ, the reproducibility and scale-up problems of selectively bound uranium proteins in the prior art are solved, and the capture and recovery of selectively bound uranium proteins are achieved efficient and repeatable capture and recovery effects are achieved.

CN114364689BActive Publication Date: 2025-07-18COMMISSARIAT A LENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES +2
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Patent Information

Application Number
CN202080063980.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Priority Date
2019-09-12
Filing Date
2020-09-07
Publication Date
2025-07-18
Estimated Expiration
2040-09-07

AI Technical Summary

Technical Problem

The prior art is difficult to capture and recover selectively binding uranium proteins in miniaturized analysis systems efficiently and reproducibly, and there are reproducibility problems with microbead carriers and difficulties in maintaining glass frits.

Method used

Capture and recovery of selectively bound uranium proteins by preparing a monolithic carrier in the channels of a miniaturization analysis system, using polymerization solutions of phosphate groups, crosslinking agents, solvents and free radical polymerization initiators.

Benefits of technology

It realizes efficient and repeatable selective capture and recycling of selectively bound uranium proteins in miniaturized analysis systems, reducing solvent and reagent consumption, reducing the limitations of radioactive sample treatment and waste management costs.

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Abstract

The present invention relates to a method for preparing a monolithic support on which uranyl cations are immobilized in the internal volume of at least one channel. According to the present invention, such a method comprises: (a) activating the inner surface of the channel; (b) introducing into the internal volume of the channel a polymerization solution comprising: - a monomer comprising a phosphoric acid group, - at least one crosslinking agent, - several solvents, and - a free radical polymerization initiator; (c) polymerizing the polymerization solution; (d) rinsing the monolithic support obtained in step (c); and (e) contacting the previously rinsed monolithic support with a solution comprising uranyl cations. The present invention also relates to a method for capturing proteins that selectively bind uranium by means of a monolithic support prepared by the above method, and to a method for recovering proteins that selectively bind uranium using the capture method.
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