Microfluidic artificial lung and manufacturing method thereof

Abstract

The present invention discloses a microfluidic artificial lung and a manufacturing method thereof, comprising: a substrate having at least one blood flow channel, at least one first air flow channel, and at least one intersection region between the first air flow channel and the blood flow channel; and at least one biomimetic sealing microstructure array comprising a plurality of microstructures, wherein the plurality of microstructure arrays are arranged in the intersection region to form an interface between the blood in the blood flow channel and the gas in the first air flow channel. The microfluidic artificial lung of the present invention forms a biomimetic sealing microstructure array by arranging the plurality of microstructure arrays in the intersection region, so that the blood in the blood flow channel and the gas in the first air flow channel fill the gaps between the plurality of microstructures when flowing through the intersection region, thereby forming a stable interface. This prevents mixing of the blood and the gas, thereby achieving a sealing function without requiring additional control or input, while allowing blood and gas to exchange gas at the interface.

Description

Technical Field

[0001] The present invention relates to the technical field of artificial lungs, and in particular to a microfluidic artificial lung and a manufacturing method thereof. Background Art

[0002] Mechanical ventilation is a method of mechanically assisting or replacing spontaneous breathing, but this treatment often has side effects, such as ventilation-related lung injury. Some of these adverse consequences of mechanical ventilation can be addressed using extracorporeal circulation systems. Extracorporeal circulation and oxygenation systems aim to achieve extracorporeal blood oxygenation (ECMO) and extracorporeal carbon dioxide removal (ECCO2R).

[0003] The extracorporeal circulation and oxygenation systems in the existing technology are mainly divided into two categories.

[0004] The first type is membrane oxygenators represented by hollow fiber membranes, which usually rely on the use of gas exchange membranes. Specifically, the gas exchange membrane separates the extracorporeal circulating blood from the gas chamber, and a blood (liquid)-gas exchange interface is constructed on both sides of the gas exchange membrane. The carbon dioxide in the extracorporeal circulating blood passes through the gas exchange membrane and diffuses into the gas chamber. At the same time, the oxygen in the gas chamber passes through the gas exchange membrane and diffuses into the extracorporeal circulating blood. This type of oxygenator is characterized by a large size and high flow rate. However, when they use the liquid-gas exchange interface, blood coagulation easily occurs at the interface, and membrane thrombosis is serious. At the same time, it is necessary to prevent gas from entering the extracorporeal circulating blood and causing gas embolism.

[0005] The second category is microfluidic artificial lung oxygenators, centered around the emerging microfluidics technology. Microfluidics, a technology originating from micro-electro-mechanical systems (MEMS), is used to manipulate fluids at the micro- and nanoscale. With numerous advantages, such as small size, high speed, and high precision, it has been widely used in various microdevices across various fields and is gradually extending into clinical technology development. Microfluidic diffusion devices and systems disclosed in prior art serve as models of artificial lungs. These microfluidic artificial lungs, comprising micron-scale flow channels and diffusion membranes, can overcome the shortcomings of traditional artificial lung systems. This is primarily due to their reduced diffusion distance, resulting in excellent gas exchange efficiency, which facilitates miniaturization and portability. The blood-contact surface area is further reduced, reducing the risk of thrombosis. Thanks to the sophistication of microfluidics, microfluidic artificial lungs can also incorporate blood flow pathways that more closely mimic those found in natural lungs, potentially improving biocompatibility and increasing lifespan. However, the gas exchange of the currently designed microfluidic artificial lung must be carried out through the diffusion membrane and the device wall, and the further improvement of its gas exchange efficiency is fundamentally restricted. Further thinning of the diffusion membrane and wall will bring higher instability. Summary of the Invention

[0006] The purpose of the present invention is to provide a microfluidic artificial lung and a manufacturing method thereof, so as to solve the technical problem that the current microfluidic artificial lung must exchange gas through the diffusion membrane and the device wall, and the gas exchange efficiency is difficult to be further improved.

[0007] The above-mentioned purpose of the present invention can be achieved by adopting the following technical solutions:

[0008] The present invention provides a microfluidic artificial lung, comprising: a substrate, provided with at least one blood flow channel, at least one first air flow channel, and at least one intersection area between the first air flow channel and the blood flow channel; at least one bionic sealing microstructure array, comprising a plurality of microstructures, wherein the plurality of microstructure arrays are arranged in the intersection area to form an interface between the blood in the blood flow channel and the gas in the first air flow channel.

[0009] In an embodiment of the present invention, the number of the blood flow channels, the number of the first air flow channels and the number of the bionic sealing microstructure arrays are all multiple, the substrate is further provided with a blood inlet channel and a bleeding channel, the blood inlet channel is connected to the bleeding channel through the multiple blood flow channels, the multiple first air flow channels and the multiple blood flow channels are alternately arranged, and the multiple bionic sealing microstructures are arranged at intervals and located between the multiple first air flow channels and the multiple blood flow channels.

[0010] In an embodiment of the present invention, the base body is further provided with at least one second air flow channel, and the second air flow channel is separated from the blood flow channel by an oxygen exchange membrane.

[0011] In an embodiment of the present invention, the substrate includes a first substrate and a second substrate, the first surface of the first substrate is provided with at least one blood flow groove, at least one first airflow groove and at least one intermediate groove, the bionic sealing microstructure array is located in the intermediate groove, the first surface of the second substrate is provided with at least one second airflow groove, the first surface of the first substrate and the first surface of the second substrate are adhered to each other through the oxygen exchange membrane, the blood flow groove forms the blood flow channel, the first airflow groove forms the first airflow channel, the intermediate groove forms the intersection area, and the second airflow groove forms the second airflow channel.

[0012] In an embodiment of the present invention, a plurality of the microstructures are arranged in the intersection area along the transport direction of the blood.

[0013] In an embodiment of the present invention, the distance between two adjacent microstructures is 5 μm to 500 μm.

[0014] In an embodiment of the present invention, the microstructure includes an intermediate structure, a connecting structure, and two end structures. The two end structures are connected to the intermediate structure through the connecting structure and are symmetrically arranged relative to the intermediate structure. The two end structures cooperate with the connecting structure to form a first contact portion in contact with the blood, and the two end structures cooperate with the intermediate structure to form a second contact portion in contact with the gas.

[0015] In an embodiment of the present invention, the first contact portion includes a first side surface and two second side surfaces, and the two second side surfaces are connected through the first side surface; the second contact portion includes a third side surface, two fourth side surfaces and two fifth side surfaces, and the two fifth side surfaces are connected to the third side surface through the two fourth side surfaces; the first side surface, the third side surface and the fifth side surface extend along the conveying direction and are arranged parallel to each other, the fourth side surface extends in a direction perpendicular to the conveying direction, one end of the second side surface is connected to the first side surface, and the other end of the second side surface extends obliquely toward the fifth side surface and is connected to the fifth side surface.

[0016] In an embodiment of the present invention, the width of the intermediate structure in the conveying direction is 10 μm to 300 μm.

[0017] In an embodiment of the present invention, the width of the connection structure in the conveying direction is 5 μm to 500 μm.

[0018] In an embodiment of the present invention, an included angle is formed between the end structure and the middle structure, and the included angle is 0°-180°.

[0019] In an embodiment of the present invention, the width of the end structure in the conveying direction is 0.5 μm-20 μm, and the width of the end structure in a direction perpendicular to the conveying direction is 0.5 μm-50 μm.

[0020] The present invention also provides a method for manufacturing a microfluidic artificial lung, comprising the following steps: preparing a first template; injecting a molding material into a molding cavity of the first template and solidifying it to form a first matrix; wherein, the first surface of the first matrix forms at least one blood flow groove, at least one first airflow groove, and at least one intermediate groove connecting the blood flow groove and the first airflow groove, and a bionic sealing microstructure array is formed in the intermediate groove; the first surface of the first matrix is ​​fitted with a second matrix, so that the blood flow groove forms a blood flow channel, the first airflow groove forms a first airflow channel, and the intermediate groove forms an intersection area.

[0021] In an embodiment of the present invention, the first surface of the first substrate is adhered to the first surface of the second substrate through an oxygen exchange film; the manufacturing method also includes preparing the second substrate, including the following steps: preparing a second template; injecting a molding material into a molding cavity of the second template and solidifying it to form the second substrate; wherein, at least one second airflow groove is formed on the first surface of the second substrate; laying the oxygen exchange film on the first surface of the second substrate and covering the second airflow groove, so that the second airflow groove forms a second airflow channel.

[0022] In an embodiment of the present invention, the following steps are also included: a blood flow input port and a blood flow output port connected to the blood flow channel, as well as a first air flow input port and a first air flow output port connected to the first air flow channel are opened on the second surface of the first substrate opposite to its first surface; a second air flow input port and a second air flow output port connected to the second air flow channel are opened on the second surface of the second substrate opposite to its first surface.

[0023] The characteristics and advantages of the present invention are:

[0024] The microfluidic artificial lung of the present invention transports blood with a low oxygen content through the blood flow channel and transports pure oxygen or gas with a high oxygen content through the first air flow channel. By arranging multiple microstructure arrays in an intersection area to form a bionic sealing microstructure array, the blood in the blood flow channel and the gas in the first air flow channel will fill the gaps between the multiple microstructures when flowing through the intersection area to form a stable interface, so that the blood and gas will not mix, thereby achieving a sealing function. No additional control and input are required to achieve the sealing function. At the same time, the blood and gas can exchange gases at the interface, that is, carbon dioxide in the blood diffuses into the gas from the interface, and oxygen in the gas diffuses into the blood from the interface.

[0025] The manufacturing method of the microfluidic artificial lung of the present invention can realize the application of the bionic sealing microstructure array in the microfluidic artificial lung by solidifying and molding the bionic sealing microstructure array with the first substrate as an integral unit, and then bonding it with the second substrate. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0027] Figure 1 Schematic diagram of the structure of the microfluidic artificial lung in the present invention.

[0028] Figure 2 Schematic diagram of the structure of the bionic sealing microstructure array in the present invention.

[0029] Figure 3 Schematic diagram of the microstructure of the present invention.

[0030] Figure 4 This is a top view of the first substrate of the microfluidic artificial lung in the present invention.

[0031] Figure 5 This is a top view of the second substrate of the microfluidic artificial lung of the present invention.

[0032] Figure 6 It is a partial cross-sectional view of the microfluidic artificial lung of the present invention.

[0033] In the picture:

[0034] 100. Bionic sealing microstructure array; 11. Microstructure; 12. First contact portion; 13. Second contact portion; 14. First side surface; 15. Second side surface; 16. Third side surface; 17. Fourth side surface; 18. Fifth side surface; 19. Intermediate structure; 110. End structure; 111. Connecting structure;

[0035] 200. Blood flow channel; 21. Blood inlet channel; 22. Bleeding channel; 23. Blood input port; 24. Blood output port; 300. First airflow channel; 31. First airflow input port; 32. First airflow output port; 400. Intersection area; 41. Interface; 500. Matrix; 51. First matrix; 52. Second matrix; 53. Oxygen exchange membrane; 600. Second airflow channel; 61. Second airflow input port; 62. Second airflow output port; B. Blood; G. Gas. DETAILED DESCRIPTION

[0036] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0037] Implementation Method 1

[0038] like Figure 1 and Figure 2 As shown, the present invention provides a microfluidic artificial lung, comprising: a substrate 500, having at least one blood flow channel 200, at least one first air flow channel 300, and at least one intersection region 400 between the first air flow channel 300 and the blood flow channel 200; at least one bionic sealing microstructure array 100, comprising a plurality of microstructures 11, wherein the plurality of microstructures 11 are arrayed in the intersection region 400 to form an interface 41 between the blood B in the blood flow channel 200 and the gas G in the first air flow channel 300.

[0039] The microfluidic artificial lung of the present invention transports blood B with a low oxygen content through the blood flow channel 200 and transports pure oxygen or gas G with a high oxygen content through the first air flow channel 300. By arranging multiple microstructures 11 in an array in the intersection area 400 to form a bionic sealing microstructure array 100, the blood B in the blood flow channel 200 and the gas G in the first air flow channel 300 will fill the gaps between the multiple microstructures 11 when flowing through the intersection area 400 to form a stable interface 41, so that the blood B and the gas G will not mix, thereby achieving a sealing function, and no additional control and input are required to achieve the sealing function. At the same time, the blood B and the gas G can exchange gases at the interface 41, that is, the carbon dioxide in the blood B diffuses from the interface 41 into the gas G, and the oxygen in the gas G diffuses from the interface 41 into the blood B.

[0040] The design inspiration and parameter design of the microstructure 11 in the present invention come from the principles of bionics. The selected prototypes of the natural microstructure 11 with special wetting properties include the "lantern-shaped" microstructure of the leaf surface of Salvinia, the "T"-shaped structure of the hair of water spiders and springtails, etc. The microstructure 11 is extracted and summarized from these structures, so that the microstructure 11 can generally be "T"-shaped, umbrella-shaped, trapezoidal, triangular or "lantern-shaped", and then arranged in an array in the intersection area 400, so that the first contact parts 12 of multiple microstructures 11 are in contact with blood B, and the second contact parts 13 of multiple microstructures 11 are in contact with gas G, thereby forming a boundary surface 41 between blood B and gas G.

[0041] Specifically, when the incoming blood B flows through the intersection region 400, it expands at the biomimetic sealing microstructure array 100, forming a blood-gas interface 41 between the blood and the gas G. The morphology of the interface 41 is related to the array shape, spacing, and specific shape of the multiple microstructures 11 in the biomimetic sealing microstructure array 100. The morphology and size of the interface 41 affect the surface tension of the interface 41, which shrinks and decreases. The incoming blood B has a certain working pressure. When these two forces are balanced, the interface 41 can exist stably, thereby ensuring that the biomimetic sealing microstructure array 100 has a better sealing effect.

[0042] It can be seen that the sealing effect of the bionic sealing microstructure array 100 in the present invention is related to the stability of the interface 41, and the stability of the interface 41 is related to the surface tension of blood B and gas G at the interface 41 and the working pressure of blood B and gas G.

[0043] The surface tension between blood B and gas G at interface 41 is related to the shape, spacing, and array configuration of microstructures 11. Therefore, the shape, spacing, and array configuration of microstructures 11 in the present invention are not specifically limited and can be adjusted based on surface tension requirements. However, for optimal implementation, the present invention provides the following preferred adjustment ranges for various parameters through theoretical calculations, simulations, and experimental verification.

[0044] like Figure 1 As shown, for the array shape, blood flow channels 200 for conveying blood B and first air flow channels 300 for conveying gas G are provided on both sides of the intersection area 400, and the conveying directions of blood B and gas G are the same. The multiple microstructures 11 can be arranged side by side in a straight line along the conveying direction X; or they can be arranged in a wavy line in an alternating manner along the conveying direction X; or the multiple microstructures 11 can be arranged into multiple columns of microstructures 11 perpendicular to the conveying direction X, and the multiple columns of microstructures 11 are arranged along the conveying direction X; of course, other arrangements are also possible, as long as it is ensured that the multiple microstructures 11 can continue to intercept the fluids on both sides in the intersection area 400 like a "guardrail", thereby achieving sealing.

[0045] like Figure 2 As shown, a preferred range of the interval L between the plurality of microstructures 11 is 5 μm to 500 μm.

[0046] like Figure 2 As shown, with respect to the shape of the microstructure 11, in order to make the shape of the microstructure 11 more specific, the present invention further extracts and summarizes that the microstructure 11 includes an intermediate structure 19, a connecting structure 111, and two end structures 110. The two end structures 110 are connected to the intermediate structure 19 via the connecting structure 111 and are symmetrical relative to the intermediate structure 19. The two end structures 110 cooperate with the connecting structure 111 to form a first contact portion 12, and the two end structures 110 cooperate with the intermediate structure 19 to form a second contact portion 13. The preferred shapes and sizes of the intermediate structure 19, the connecting structure 111, and the end structures 110 are provided below, so that sufficient surface tension can be provided at the bionic sealing microstructure array 100, thereby achieving a better sealing effect.

[0047] like Figure 3As shown, specifically, the cross-sectional shape of the intermediate structure 19 along the horizontal direction is rectangular, the cross-sectional shape of the connecting structure 111 along the horizontal direction is rectangular, and the end of the end structure 110 adjacent to the adjacent microstructure 11 is a sharp angle of any angle. The end structure 110 and the intermediate structure 19 form an angle, and the angle is 0° to 180°. The width W1 of the intermediate structure 19 in the conveying direction is 10μm to 300μm. The width W2 of the connecting structure 111 in the conveying direction is 5μm to 500μm. The width W3 of the end structure 110 in the conveying direction X is 0.5μm-20μm, and the width W4 of the end structure 110 in the direction perpendicular to the conveying direction X is 0.5μm-50μm. In this embodiment, the angle between the end structure 110 and the intermediate structure 19 is 90 degrees, and the cross-sectional shape of the end structure 110 along the horizontal direction is a right-angled trapezoid. The first contact portion 12 includes a first side surface 14 and two second side surfaces 15, and the two second side surfaces 15 are connected through the first side surface 14; the second contact portion 13 includes a third side surface 16, two fourth side surfaces 17 and two fifth side surfaces 18, and the two fifth side surfaces 18 are connected to the third side surface 16 through the two fourth side surfaces 17; the first side surface 14, the third side surface 16 and the fifth side surface 18 extend along the conveying direction X and are arranged parallel to each other, and the fourth side surface 17 extends in a direction perpendicular to the conveying direction X. One end of the second side surface 15 is connected to the first side surface 14, and the other end of the second side surface 15 extends obliquely toward the fifth side surface 18 and is connected to the fifth side surface 18.

[0048] Alternatively, the cross-sectional shape of the intermediate structure may be trapezoidal, triangular, elliptical, or other irregular shapes. The cross-sectional shape of the connecting structure may also be trapezoidal, circular, elliptical, or other irregular shapes. The end of the end structure adjacent to the adjacent microstructure may also be rounded or right-angled.

[0049] like Figure 1 As shown, the working pressure of blood B and gas G can be regulated within the range of 0-100 kPa (gauge pressure) so that the surface tension provided by the interface 41 formed at the bionic sealing microstructure array 100 is balanced with the working pressure of blood B and gas G.

[0050] like Figure 2 As shown, microstructure 11 is made of materials such as PDMS (polydimethylsiloxane), glass, or acrylic. In an embodiment of the present invention, to enable the biomimetic sealing microstructure array 100 to better separate and seal blood B and gas G, the first contact portion 12 is provided with a hydrophilic layer formed by a hydrophilic treatment agent. Furthermore, the second contact portion 13 is provided with a hydrophobic layer formed by a hydrophobic treatment agent.

[0051] like Figure 4As shown, in an embodiment of the present invention, the number of blood flow channels 200, the number of first air flow channels 300, and the number of biomimetic sealing microstructure arrays 100 are all multiple. The base 500 is further provided with a blood inlet channel 21 and a bleeding channel 22. The blood inlet channel 21 is connected to the bleeding channel 22 through the multiple blood flow channels 200. The multiple first air flow channels 300 and the multiple blood flow channels 200 are arranged alternately, and the multiple biomimetic sealing microstructure arrays 100 are arranged at intervals and located between the multiple first air flow channels 300 and the multiple blood flow channels 200. Blood B enters the multiple blood flow channels 200 from the blood inlet channel 21, and gas G flows into the multiple first air flow channels 300 respectively. As a result, the blood B and gas G form a stable interface 41 at the multiple biomimetic sealing microstructure arrays 100, allowing gas exchange. This further improves the efficiency of gas exchange and increases the contact area between blood B and oxygen.

[0052] Specifically, the base 500 is provided with a blood input port 23 connected to the blood inlet channel 21, a blood output port 24 connected to the bleeding channel 22, a plurality of first airflow input ports 31 connected to one end of the plurality of first airflow channels 300, and a plurality of first airflow output ports 32 connected to the other end of the plurality of first airflow channels 300.

[0053] like Figure 5 and Figure 6 As shown, to further increase the contact area between blood B and oxygen in the microfluidic artificial lung, in an embodiment of the present invention, the base 500 is further provided with at least one second airflow channel 600, which is separated from the blood flow channel 200 by an oxygen exchange membrane 53. The blood B in the blood flow channel 200 can exchange gas with the gas G in the first airflow channels 300 on either side horizontally at the biomimetic sealing microstructure arrays 100 on either side, and vertically with the gas G in the second airflow channel 600 through the oxygen exchange membrane 53. In this embodiment, the second airflow channel 600 is generally grid-shaped and laid below multiple blood flow channels 200, thereby enabling simultaneous gas exchange with the blood B in multiple blood flow channels 200.

[0054] Optionally, the base is also provided with a third air flow channel, which is also separated from the blood flow channel by an oxygen exchange membrane. The third air flow channel is generally in the shape of a grid and is laid above multiple blood flow channels, so that the blood in the blood flow channel can exchange gas with the gas above, below, left and right of it.

[0055] Since the blood flow channel 200, the first air flow channel 300, the biomimetic sealing microstructure array 100, and the second air flow channel 600 all need to be manufactured inside the microfluidic artificial lung, in order to reduce the manufacturing difficulty, in an embodiment of the present invention, the substrate 500 includes a first substrate 51 and a second substrate 52. The first surface of the first substrate 51 is provided with at least one blood flow groove, at least one first air flow groove, and at least one intermediate groove. The biomimetic sealing microstructure array 100 is located in the intermediate groove. The first surface of the second substrate 52 is provided with at least one second air flow groove. The first surface of the first substrate 51 and the first surface of the second substrate 52 are attached to each other through an oxygen exchange membrane 53. The blood flow groove forms the blood flow channel 200, the first air flow groove forms the first air flow channel 300, the intermediate groove forms the intersection area 400, and the second air flow groove forms the second air flow channel 600. The specific manufacturing steps of the microfluidic artificial lung are described in detail in the second embodiment and will not be described in detail here.

[0056] Implementation Method 2

[0057] like Figure 4 、 Figure 5 as well as Figure 6 As shown, the present invention also provides a method for manufacturing a microfluidic artificial lung, comprising the following steps: preparing a first template; injecting a molding material into the molding cavity of the first template and solidifying it to form a first substrate 51; wherein the first surface of the first substrate 51 is formed with at least one blood flow groove, at least one first airflow groove, and at least one intermediate groove connecting the blood flow groove and the first airflow groove, and a biomimetic sealing microstructure array 100 is formed in the intermediate groove; and the first surface of the first substrate 51 is bonded to the second substrate 52, so that the blood flow groove forms a blood flow channel 200, the first airflow groove forms a first airflow channel 300, and the intermediate groove forms an intersection area 400. The specific structure, working principle, and beneficial effects of the microfluidic artificial lung in this embodiment are the same as those of the microfluidic artificial lung in embodiment 1, and will not be repeated here.

[0058] The manufacturing method of the microfluidic artificial lung of the present invention is to solidify and mold the bionic sealing microstructure array 100 and the first substrate 51 as an integral whole, and then bond them to the second substrate 52, so that the bionic sealing microstructure array 100 is manufactured inside the microfluidic artificial lung, and the blood flow channel 200 and the first air flow channel 300 are formed inside the microfluidic artificial lung, and the operation is simple.

[0059] like Figure 5 and Figure 6As shown, in an embodiment of the present invention, the first surface of the first substrate 51 is bonded to the first surface of the second substrate 52 through the oxygen exchange film 53; the manufacturing method also includes preparing the second substrate 52, including the following steps: preparing a second template; injecting a molding material into the molding cavity of the second template and solidifying it to form the second substrate 52; wherein, at least one second airflow groove is formed on the first surface of the second substrate 52; laying the oxygen exchange film 53 on the first surface of the second substrate 52 and covering the second airflow groove, so that the second airflow groove forms a second airflow channel 600.

[0060] Specifically, both the first and second templates can be fabricated from silicon wafers through soft photolithography. The molding material is PDMS (polydimethylsiloxane), glass, or acrylic. The first substrate 51, oxygen exchange membrane 53, and second substrate 52 can be connected using plasma bonding.

[0061] like Figure 4 and Figure 5 As shown, in an embodiment of the present invention, the following steps are also included: a blood input port 23 and a blood output port 24 communicating with the blood flow channel 200, as well as a first air flow input port 31 and a first air flow output port 32 communicating with the first air flow channel 300 are opened on the second surface (i.e., the top surface of the first substrate 51) of the first substrate 51 opposite to its first surface (i.e., the bottom surface of the first substrate 51), and a second air flow input port 61 and a second air flow output port 62 communicating with the second air flow channel 600 are opened on the second surface (i.e., the bottom surface of the second substrate 52) of the second substrate 52 opposite to its first surface (i.e., the top surface of the second substrate 52).

[0062] The above descriptions are only several embodiments of the present invention. Those skilled in the art may make various changes or modifications to the embodiments of the present invention based on the contents disclosed in the application documents without departing from the spirit and scope of the present invention.

Claims

1. A microfluidic artificial lung, characterized in that: include: A base body, having at least one blood flow channel, at least one first air flow channel, and at least one intersection area between the first air flow channel and the blood flow channel; at least one biomimetic sealing microstructure array, comprising a plurality of microstructures, wherein the plurality of microstructure arrays are arranged in the intersection area to form an interface between the blood in the blood flow channel and the gas in the first gas flow channel; The plurality of microstructures are arranged in the intersection area along the blood transport direction; The microstructure includes a middle structure, a connecting structure, and two end structures, wherein the two end structures are connected to the middle structure via the connecting structure and are symmetrically arranged relative to the middle structure, the two end structures cooperate with the connecting structure to form a first contact portion in contact with the blood, and the two end structures cooperate with the middle structure to form a second contact portion in contact with the gas; The surface tension provided by the interface formed at the bionic sealing microstructure array is balanced with the working pressure of the blood and the gas, so that the blood and the gas will not mix, while the blood and the gas can exchange gases at the interface.

2. The microfluidic artificial lung according to claim 1, characterized in that The number of the blood flow channels, the number of the first air flow channels and the number of the bionic sealing microstructure arrays are all multiple. The substrate is also provided with a blood inlet channel and a bleeding channel. The blood inlet channel is connected to the bleeding channel through the multiple blood flow channels. The multiple first air flow channels and the multiple blood flow channels are arranged alternately, and the multiple bionic sealing microstructures are arranged at intervals and located between the multiple first air flow channels and the multiple blood flow channels.

3. The microfluidic artificial lung according to claim 1, characterized in that: The base body is further provided with at least one second air flow channel, and the second air flow channel is separated from the blood flow channel by an oxygen exchange membrane.

4. The microfluidic artificial lung according to claim 3, characterized in that: The substrate includes a first substrate and a second substrate. The first surface of the first substrate is provided with at least one blood flow groove, at least one first airflow groove and at least one intermediate groove. The bionic sealing microstructure array is located in the intermediate groove. The first surface of the second substrate is provided with at least one second airflow groove. The first surface of the first substrate and the first surface of the second substrate are bonded together through the oxygen exchange membrane. The blood flow groove forms the blood flow channel, the first airflow groove forms the first airflow channel, the intermediate groove forms the intersection area, and the second airflow groove forms the second airflow channel.

5. The microfluidic artificial lung according to claim 1, characterized in that The distance between two adjacent microstructures is 5 μm to 500 μm.

6. The microfluidic artificial lung according to claim 1, characterized in that The first contact portion includes a first side surface and two second side surfaces, and the two second side surfaces are connected through the first side surface; the second contact portion includes a third side surface, two fourth side surfaces, and two fifth side surfaces, and the two fifth side surfaces are connected to the third side surface through the two fourth side surfaces; The first side surface, the third side surface, and the fifth side surface extend along the conveying direction and are arranged parallel to each other. The fourth side surface extends in a direction perpendicular to the conveying direction. One end of the second side surface is connected to the first side surface, and the other end of the second side surface extends obliquely toward the fifth side surface and is connected to the fifth side surface.

7. The microfluidic artificial lung according to claim 1, characterized in that: The width of the intermediate structure in the conveying direction is 10 μm to 300 μm.

8. The microfluidic artificial lung according to claim 1, characterized in that The width of the connecting structure in the conveying direction is 5 μm to 500 μm.

9. The microfluidic artificial lung according to claim 1, characterized in that: An included angle is formed between the end structure and the middle structure, and the included angle is 0°-180°.

10. The microfluidic artificial lung according to claim 1, characterized in that: The width of the end structure in the conveying direction is 0.5 μm-20 μm, and the width of the end structure in a direction perpendicular to the conveying direction is 0.5 μm-50 μm.

11. A method for manufacturing a microfluidic artificial lung, for manufacturing the microfluidic artificial lung according to any one of claims 1 to 10, characterized in that: The manufacturing method comprises the following steps: preparing a first template; Injecting a molding material into the molding cavity of the first template and solidifying the material to form a first matrix; wherein the first surface of the first matrix is ​​formed with at least one blood flow groove, at least one first air flow groove, and at least one intermediate groove connecting the blood flow groove and the first air flow groove, and a bionic sealing microstructure array is formed in the intermediate groove; at least one biomimetic sealing microstructure array, comprising a plurality of microstructures, wherein the plurality of microstructure arrays are arranged in the intersection area to form an interface between the blood in the blood flow channel and the gas in the first gas flow channel; The microstructure includes a middle structure, a connecting structure, and two end structures, wherein the two end structures are connected to the middle structure via the connecting structure and are symmetrically arranged relative to the middle structure, the two end structures cooperate with the connecting structure to form a first contact portion in contact with the blood, and the two end structures cooperate with the middle structure to form a second contact portion in contact with the gas; The first surface of the first substrate is fitted to the second substrate, so that the blood flow groove forms a blood flow channel, the first air flow groove forms a first air flow channel, and the middle groove forms a junction area.

12. The method for manufacturing a microfluidic artificial lung according to claim 11, characterized in that: The first surface of the first substrate is bonded to the first surface of the second substrate via an oxygen exchange film; the manufacturing method further includes preparing the second substrate, including the following steps: preparing a second template; Injecting a molding material into the molding cavity of the second template and solidifying the molded material to form the second substrate; wherein at least one second airflow groove is formed on the first surface of the second substrate; The oxygen exchange membrane is laid on the first surface of the second substrate and covers the second air flow groove, so that the second air flow groove forms a second air flow channel.

13. The method for manufacturing a microfluidic artificial lung according to claim 12, characterized in that: The following steps are also included: A blood flow inlet and a blood flow outlet communicated with the blood flow channel, and a first air flow inlet and a first air flow outlet communicated with the first air flow channel are provided on a second surface of the first base body opposite to the first surface; A second airflow inlet and a second airflow outlet communicating with the second airflow channel are provided on a second surface of the second base body opposite to the first surface.

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