Liquid dispensing apparatus and method and use thereof

Abstract

The application discloses a liquid dispensing device and method and application thereof. The device comprises a main body, which comprises a pressing plate, a liquid dispensing cavity and a playback liquid part penetrating through the liquid dispensing cavity arranged from top to bottom; the liquid dispensing cavity and the playback liquid part are closeable or separable, when the liquid dispensing cavity and the playback liquid part are closed, the device is used for quantitative dispensing of a liquid to be dispensed and storage of the quantitatively dispensed liquid; the pressing plate is used for separating the liquid dispensing cavity and the playback liquid part by applying external force, and the stored quantitatively dispensed liquid flows along the outer wall of the playback liquid part downward and flows to a liquid transfer plate. The liquid dispensing device and method can continuously and efficiently divide and dispense the liquid, and the device has a lower cost.

Description

Technical Field

[0001] This invention relates to a liquid dispensing apparatus and method, and their applications. Background Technology

[0002] Liquid dispensing equipment is mainly used for the quantitative transfer and equal distribution of liquids to a pipette. This primarily includes the quantitative aspiration, directional transfer, and discharge of solutions. Existing pipetting equipment mainly consists of manual and automatic single-channel and multi-channel pipetting devices.

[0003] Commonly used manual single-channel and multi-channel pipetting devices mainly include pipettes and multi-channel pipettes. For example, using an 8-channel multi-channel pipette to complete the liquid dispensing operation of a 96-well plate requires 12 operations, which is time-consuming and labor-intensive.

[0004] Commonly used automatic multichannel pipetting devices, such as CN217288466U, disclose a miniaturized twelve-channel high-precision dispensing device, which includes a base (1), a housing (2), and an extraction tube fixing platform (3). The device is characterized by: a multichannel dispensing assembly (4) being fitted inside the housing (2). The multichannel dispensing assembly (4) includes a solution transfer tank (401), an inlet pipe (402), and nozzles (403). The solution transfer tank (401) has equidistantly spaced cavities inside. Two adjacent sets of nozzles (403) are fixedly connected to the bottom of the cavities. Adjusting rods (405) are symmetrically arranged on both sides of the solution transfer tank (401). The nozzles (403) are fixedly connected to a peristaltic pump (6) via pipes. The extraction tube fixing platform (3) is slidably installed inside the housing (2). A drive assembly (5) is provided on the bottom surface of the housing (2). The extraction tube fixing platform (3) includes a connecting plate (301), a screw (302) and an extraction tube support plate (303). The extraction tube support plate (303) has positioning holes (304) arranged at equal intervals. The extraction tube support plate (303) is fixedly installed on the top of the screw (302). The screw (302) is threadedly connected to the connecting plate (301). The driving assembly (5) includes a lead screw (501), a motor (502) and a slider (503). A screw sleeve (504) is rotatably installed in the slider (503) through a bearing. The screw sleeve (504) is threadedly connected to the surface of the lead screw (501). The motor (502) is fixedly connected to one end of the lead screw (501) through a coupling. The lead screw (501) is rotatably connected to the housing (2). The connecting plate (301) is fixedly installed on the upper surface of the slider (503). In multichannel pipetting devices, the most common approach is to use a drive assembly, but this structure requires a high degree of automation and has a high equipment cost.

[0005] In summary, existing liquid dispensing equipment mainly suffers from problems such as time-consuming and labor-intensive operation and high equipment costs. Summary of the Invention

[0006] To address the problems existing in the prior art, the present invention provides a liquid dispensing device and method. The liquid dispensing device of the present invention can continuously and uniformly dispense liquids, and is highly efficient, labor-saving, and has a lower equipment cost.

[0007] The first aspect of the present invention provides a liquid dispensing device, which includes a main body 2. The main body 2 includes a pressure plate 21 arranged from top to bottom, a dispensing chamber 22, and a liquid return component 23 penetrating the dispensing chamber 22. The dispensing chamber 22 and the liquid return component 23 can be sealed or separated. When the dispensing chamber 22 and the liquid return component 23 are sealed, they are used to quantitatively dispense and store the quantitatively dispensed liquid. The pressure plate 21 is used to separate the dispensing chamber 22 from the liquid return component 23 by applying external force. The quantitatively dispensed liquid stored in the dispensing chamber 22 flows downward along the outer wall of the liquid return component 23 and flows to the pipette plate 4.

[0008] According to the liquid dispensing apparatus of the present invention, the liquid dispensing apparatus can be used for continuous production operations such as batch testing of medical reagents, for example, dispensing liquids, especially dispensing liquids into well plates, such as, but not limited to, 24-well plates, 96-well plates or 384-well plates.

[0009] According to the liquid dispensing device of the present invention, preferably, the dispensing chamber 22 is provided with a plurality of holes, each hole including a boss 221, a flow channel 222 and a liquid storage component 223; wherein, the boss 221 protrudes from the opening of the hole to prevent the liquid to be dispensed from flowing into the hole; the flow channel 222 is formed on the boss 221 to allow the liquid to be dispensed to flow into the hole for quantitative dispensing to form quantitatively dispensed liquid. In the present invention, the number of holes is determined as needed; for example, when the dispensing target is a 96-well plate, 96 holes can be provided accordingly.

[0010] According to the liquid dispensing device of the present invention, preferably, the liquid return component 23 includes a plurality of tubes 231 that match a plurality of holes in the dispensing chamber 22, each tube 231 having a liquid-locking component 232 on its sidewall, and each tube 231 having a tapered component 233 at its lower end; the top of each tube 231 contacts the pressure plate 21; the liquid-locking component 232 is used to seal and connect with the boss 221 and prevent the liquid to be dispensed from flowing into the holes.

[0011] According to the liquid dispensing device of the present invention, when the dispensing chamber 22 and the return liquid component 23 are sealed, the liquid to be dispensed enters the orifice through the flow channel 222, completing the quantitative dispensing of the liquid and storing it in the space formed by the storage component 223 and the return liquid component 23. In the present invention, the volume of the space formed by the storage component 223 and the return liquid component 23 is the same as the target volume of each orifice of the dispensing target. For example, if the target volume of each orifice of a 96-well plate is 250 μL, then the volume of the space formed by each storage component 223 and the return liquid component 23 is 250 μL.

[0012] According to the liquid dispensing device of the present invention, preferably, the top of each tube 231 is in contact with the pressure plate 21.

[0013] According to the liquid dispensing device of the present invention, preferably, the liquid-locking component 232 is used to seal and connect with the boss 221 to prevent the liquid to be dispensed from flowing into the hole.

[0014] According to the liquid dispensing device of the present invention, preferably, when the dispensing chamber 22 and the return liquid component 23 are in a closed state, the quantitatively dispensed liquid is stored in the space formed by the storage component 223 and the return liquid component 23. For example, when an external force presses the pressure plate 21, the tube 231 in contact with the pressure plate 21 moves downward, and the dispensing chamber 22 and the return liquid component 23 separate. The liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4, completing the quantitative dispensing of the liquid. In the present invention, the pipette plate 4 is the dispensing target, for example, but not limited to, a 24-well plate, a 96-well plate, or a 384-well plate.

[0015] According to the liquid dispensing device of the present invention, preferably, the diameter of the liquid-locking component 232 is larger than the diameter of the boss 221. When an external force presses down on the pressure plate 21, the tube body 231 in contact with the pressure plate 21 faces downward. At this time, the liquid-locking component 232 faces downward and is sealed to the boss 221, thereby preventing the liquid to be dispensed from flowing into the hole.

[0016] In the liquid dispensing device according to the present invention, preferably, the liquid-locking component 232 is provided with an exhaust port.

[0017] In the liquid dispensing device according to the present invention, preferably, a sealing ring is provided inside the liquid-locking component 232.

[0018] According to the liquid dispensing device of the present invention, preferably, a sealing ring 234 is provided on the outer wall of the conical component 233. The sealing ring provided on the outer wall of the conical component 233 is used to ensure the airtightness of the space formed by the liquid storage component 223 and the liquid return component 23.

[0019] According to the liquid dispensing apparatus of the present invention, preferably, the liquid dispensing apparatus further includes a spring 24 for supporting the pressure plate 21. The position of the spring can be determined as needed to support the pressure plate 21.

[0020] According to the liquid dispensing device of the present invention, the pressure plate 21 may be provided with a plurality of holes that match the plurality of holes in the dispensing chamber 22, depending on the need.

[0021] According to one embodiment of the present invention, the liquid dispensing device includes, but is not limited to, a main body 2, the main body 2 including, from top to bottom, a pressure plate 21, a dispensing chamber 22, and a return liquid component 23 penetrating through the dispensing chamber 22, wherein the dispensing chamber 22 and the return liquid component 23 can be sealed or separable. When the dispensing chamber 22 and the return liquid component 23 are sealed, it is used to quantitatively dispense the liquid to be dispensed and store the quantitatively dispensed liquid; the pressure plate 21 is used to separate the dispensing chamber 22 and the return liquid component 23 by applying external force, and the stored quantitatively dispensed liquid flows downward along the outer wall of the return liquid component 23 and flows to the pipette plate 4;

[0022] The liquid distribution chamber 22 is provided with multiple holes, each hole including a boss 221, a flow groove 222 and a liquid storage component 223; wherein, the boss 221 protrudes from the opening of the hole to prevent the liquid to be distributed from flowing into the hole; the flow groove 222 is formed on the boss 221 to allow the liquid to be distributed to flow into the hole for quantitative distribution to form quantitatively distributed liquid;

[0023] The liquid return component 23 includes multiple tubes 231 that match the multiple holes of the dispensing chamber 22, each tube 231 has a liquid-locking component 232 on its side wall, and each tube 231 has a tapered component 233 at its lower end; the top of each tube 231 contacts the pressure plate 21; the liquid-locking component 232 is used to seal and connect with the boss 221 and prevent the liquid to be dispensed from flowing into the hole; the liquid-locking component 232 has an exhaust hole, the liquid-locking component 232 has a sealing ring inside, and the tapered component 233 has a sealing ring 234 on its outer wall; the liquid dispensing device also includes a spring 24 for supporting the pressure plate 21.

[0024] When the pressure plate 21 is pressed by an external force, the spring is compressed, the tube 231 in contact with the pressure plate 21 moves downward, and the entire liquid return component 23 moves downward. At this time, the dispensing chamber 22 separates from the liquid return component 23. The liquid stored in the space formed by the storage component 223 and the liquid return component 23 flows downward along the outer wall of the conical component 233 and flows to the transfer plate 4. At the same time, the liquid locking component 232 of the liquid return component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the hole, ensuring quantitative and uniform liquid dispensing. When the external force is released, the spring 24 springs up, the tube 231 in contact with the pressure plate 21 moves upward, and the entire liquid return component 23 penetrating the dispensing chamber 22 moves upward, making the dispensing chamber 22 and the liquid return component 23 sealed. The liquid to be dispensed enters the hole through the flow channel 222, completing the quantitative liquid dispensing and storage in the space formed by the storage component 223 and the liquid return component 23. When the pipette plate 4 is replaced and the pressure plate 21 is pressed down again, the spring is compressed, and the tube 231 in contact with the pressure plate 21 moves downward, thereby separating the dispensing chamber 22 from the return liquid component 23. The liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4. At the same time, the liquid-locking component 232 of the return liquid component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the hole, ensuring quantitative and uniform liquid dispensing. By repeating this process multiple times as needed, the liquid dispensing device of the present invention can continuously dispense liquid.

[0025] The second aspect of the present invention provides a method for liquid dispensing using the above-described liquid dispensing equipment. The method includes: pouring the liquid to be dispensed into the dispensing chamber 22, dispensing and storing it quantitatively in the dispensing chamber 22, separating the dispensing chamber 22 from the return liquid component 23 by applying external force, and allowing the stored liquid to flow downward along the outer wall of the return liquid component 23 and flow to the pipette plate 4.

[0026] According to the liquid dispensing method of the present invention, for example, at the start of liquid dispensing, the liquid to be dispensed is poured into the dispensing chamber 22 through the liquid storage container 5.

[0027] According to the liquid dispensing method of the present invention, for example, after the liquid dispensing is completed, the remaining liquid in the dispensing chamber 22 can be poured out.

[0028] In the liquid dispensing method according to the present invention, preferably, the external force is selected from mechanical external force, airtight external force, or automated external force. Automated external force can be an external force controlled by an operating system such as PLC or DCS. Considering cost, mechanical external force or airtight external force is preferred. For example, the external force can be manually applied.

[0029] According to a specific embodiment of the present invention, the liquid dispensing method includes, but is not limited to: pouring the liquid to be dispensed into the dispensing chamber 22, the dispensing chamber 22 being provided with multiple holes, each hole being provided with a boss 221, a flow groove 222, and a liquid storage component 223; when the dispensing chamber 22 and the return liquid component 23 are in a closed state, in the dispensing chamber, the liquid to be dispensed flows into the holes through the flow groove 222 and is quantitatively dispensed to form a quantitatively dispensed liquid, the quantitatively dispensed liquid is stored in the liquid storage component 223 and the return liquid component 23. Within the space; spring 24 supports pressure plate 21, external force presses pressure plate 21, tube 231 in contact with pressure plate 21 moves downward, and return liquid component 23 moves downward as a whole. At this time, the dispensing chamber 22 separates from the return liquid component 23, and the liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the transfer plate 4. At the same time, the liquid-locking component 232 of the return liquid component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the hole, ensuring quantitative and uniform liquid dispensing. When the external force is removed, spring 24 springs up, tube 231 in contact with pressure plate 21 moves upward, and the return liquid component 23 penetrating the dispensing chamber 22 moves upward as a whole, so that the dispensing chamber 22 and the return liquid component 23 are sealed. The liquid to be dispensed enters the hole through the flow groove 222, completing the quantitative liquid dispensing and storage in the space formed by the storage component 223 and the return liquid component 23. When the pipette plate 4 is replaced and the pressure plate 21 is pressed down again, the spring is compressed, and the tube 231 in contact with the pressure plate 21 moves downward, thereby separating the dispensing chamber 22 from the return liquid component 23. The liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4. At the same time, the liquid-locking component 232 of the return liquid component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the hole, ensuring quantitative and uniform liquid dispensing. By repeating this process multiple times as needed, the liquid dispensing device of the present invention can continuously dispense liquid.

[0030] The third aspect of the present invention provides the application of the above-described liquid dispensing apparatus or the above-described liquid dispensing method in biology and chemistry.

[0031] This invention has at least one of the following advantages:

[0032] 1. Existing automated multichannel pipetting equipment is relatively expensive, costing approximately 100,000 yuan per unit. It also requires the use of pipette tips and corresponding accessories from the manufacturer. If any of the related components malfunction and affect the use of the equipment, the equipment cost and processing fees will be relatively high. However, the equipment of this invention can significantly reduce the cost of the equipment to only a few thousand yuan or less. The manufacturing cost is also lower, and the cost of the components is lower and they are easy to replace.

[0033] 2. The liquid dispensing equipment and method of the present invention can continuously transfer liquid and distribute liquid evenly, which has the advantages of simple operation, saving time and effort, and can greatly reduce operating costs.

[0034] Additional aspects and advantages of the invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description

[0035] Figure 1 This is a schematic diagram showing the disassembly of the liquid dispensing equipment according to Embodiment 1 of the present invention;

[0036] Figure 2 This is a schematic diagram of the liquid dispensing equipment after assembly according to Embodiment 1 of the present invention;

[0037] Figure 3 This is a cross-sectional view of the liquid return component of the liquid dispensing device according to Embodiment 1 of the present invention;

[0038] Figure 4 This is a cross-sectional view of the dispensing chamber of the liquid dispensing device according to Embodiment 1 of the present invention.

[0039] Among them, 2-main body; 21-pressure plate; 22-dispensing chamber; 23-liquid return component; 24-spring; 221-bore; 222-flow groove; 223-liquid storage component; 231-pipe body; 232-liquid locking component; 233-conical component; 234-sealing ring; 4-pipette; 5-liquid storage container. Detailed Implementation

[0040] The present invention will now be described with reference to specific embodiments. It should be noted that these embodiments are merely illustrative and should not be construed as limiting the present invention.

[0041] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. Identical components are indicated by the same reference numerals. It should be noted that the terms "front," "rear," "left," "right," "up," and "down" used in the following description refer to directions in the accompanying drawings, while the terms "inner" and "outer" refer to directions toward or away from the geometric center of a specific component, respectively.

[0042] Example 1

[0043] use Figure 1-4The liquid dispensing device shown has a main body 2, which has a pressure plate 21, a dispensing chamber 22, and a return liquid component 23 extending through the dispensing chamber 22 from top to bottom. The dispensing chamber 22 and the return liquid component 23 can be sealed or separated. When the dispensing chamber 22 and the return liquid component 23 are sealed, they are used to quantitatively dispense and store the quantitatively dispensed liquid. The pressure plate 21 is used to separate the dispensing chamber 22 and the return liquid component 23 by applying external force. The stored quantitatively dispensed liquid flows downward along the outer wall of the return liquid component 23 and flows to the pipette plate 4.

[0044] The dispensing chamber 22 has 96 holes, each hole having a boss 221, a flow channel 222, and a storage component 223. The boss 221 protrudes from the opening of the hole to prevent the liquid to be dispensed from flowing into the hole. The flow channel 222 is formed on the boss 221 to allow the liquid to be dispensed to flow into the hole for quantitative dispensing. When the dispensing chamber 22 and the return liquid component 23 are in a closed state, the quantitatively dispensed liquid is stored in the space formed by the storage component 223 and the return liquid component 23 (the volume of the space formed by each storage component 223 and the return liquid component 23 is 250 μL).

[0045] The liquid return component 23 has 96 tubes 231 that match the 96 holes of the dispensing chamber 22. Each tube 231 has a liquid-locking component 232 on its side wall and a tapered component 233 at its lower end. The top of each tube 231 contacts the pressure plate 21. The liquid-locking component 232 is used to seal the connection with the boss 221 and prevent the liquid to be dispensed from flowing into the holes. The liquid-locking component 232 has an exhaust hole, a sealing ring inside the liquid-locking component 232, and a sealing ring 234 on the outer wall of the tapered component 233. The liquid dispensing device also includes a spring 24 for supporting the pressure plate 21.

[0046] Example 2

[0047] Liquid dispensing is performed using the liquid dispensing equipment of Example 1. The liquid to be dispensed in the storage container 5 is poured into the dispensing chamber 22. When the dispensing chamber 22 and the return liquid component 23 are in a closed state, the liquid to be dispensed flows into the orifice through the flow channel 222 and is quantitatively dispensed. The quantitatively dispensed liquid is stored in the space formed by the storage component 223 and the return liquid component 23 (the volume of the space formed by each storage component 223 and the return liquid component 23 is 250 μL). The spring 24 supports the pressure plate. 21. When the pressure plate 21 is pressed by manual force, the tube 231 in contact with the pressure plate 21 faces downwards, and the entire return liquid component 23 faces downwards. At this time, the dispensing chamber 22 and the return liquid component 23 are separated. The liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downwards along the outer wall of the conical component 233 and flows to the pipetting plate 4 (96-hole plate). At the same time, the liquid-locking component 232 of the return liquid component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the hole, ensuring quantitative and uniform liquid dispensing. When the manual force is released, the spring 24 springs up, the tube 231 in contact with the pressure plate 21 faces upwards, and the entire return liquid component 23 penetrating the dispensing chamber 22 faces upwards, making the dispensing chamber 22 and the return liquid component 23 sealed. The liquid to be dispensed enters the hole through the flow channel 222, completing the quantitative liquid dispensing and storage in the space formed by the storage component 223 and the return liquid component 23.

[0048] When the pipette plate 4 (96-well plate) is replaced and the pressure plate 21 is pressed down manually again, the spring is compressed, and the tube 231 in contact with the pressure plate 21 moves downward, thereby separating the dispensing chamber 22 from the return liquid component 23. The liquid stored in the space formed by the storage component 223 and the return liquid component 23 flows downward along the outer wall of the conical component 233 and flows to the pipette plate 4. At the same time, the liquid-locking component 232 of the return liquid component 23 is sealed to the boss 221 to prevent the liquid to be dispensed from flowing into the orifice, ensuring quantitative and uniform liquid dispensing.

[0049] Manually apply external force to press the pressure plate 10 times in a cycle to complete 10 liquid dispensing cycles of the 96-well plate. After liquid dispensing is completed, manually pour out the remaining liquid in the dispensing chamber 22.

[0050] The liquid dispensing device of the present invention can continuously dispense liquids. The volume of liquid in any 10 wells of the pipette plate 4 (96-well plate) was measured using a pipette, and it was 250 μL in all cases, indicating that the liquid dispensing device of the present invention can accurately dispense liquids quantitatively.

[0051] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0052] Although embodiments of the invention have been shown and described, those skilled in the art will understand that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents.

Claims

1. A liquid dispensing device, characterized in that, The device includes a main body (2), which includes a pressure plate (21) arranged from top to bottom, a liquid dispensing chamber (22), and a liquid return component (23) penetrating the liquid dispensing chamber (22); wherein, The liquid dispensing chamber (22) and the liquid return component (23) can be sealed or separated. When the liquid dispensing chamber (22) and the liquid return component (23) are sealed, they are used to quantitatively dispense the liquid to be dispensed and store the quantitatively dispensed liquid. The pressure plate (21) is used to separate the liquid dispensing chamber (22) from the liquid return component (23) by applying external force, and the stored quantitatively dispensed liquid flows down along the outer wall of the liquid return component (23) and flows to the pipette plate (4). The liquid separation chamber (22) is provided with multiple holes, each hole including a boss (221), a flow groove (222) and a liquid storage component (223). When the liquid distribution chamber (22) and the liquid return component (23) are in a closed state, the quantitatively distributed liquid is stored in the space formed by the liquid storage component (223) and the liquid return component (23); The boss (221) protrudes from the opening of the hole; The flow groove (222) is provided on the boss (221) to allow the liquid to be distributed to flow into the hole for quantitative distribution to form quantitatively distributed liquid; The liquid return component (23) includes multiple tubes (231) that match the multiple holes of the liquid distribution chamber (22), each tube (231) has a liquid-locking component (232) on its side wall, and each tube (231) has a tapered component (233) at its lower end. The top of the tube (231) is in contact with the pressure plate (21); The liquid-locking component (232) is used to seal and connect with the boss (221) and prevent the liquid to be dispensed from flowing into the hole; The diameter of the liquid-locking component (232) is larger than the diameter of the boss (221); The liquid-locking component (232) is provided with an exhaust port; The liquid-locking component (232) is provided with a sealing ring inside; The liquid dispensing device also includes a spring (24) for supporting the pressure plate (21).

2. The liquid dispensing equipment according to claim 1, characterized in that, A sealing ring (234) is provided on the outer wall of the tapered component (233).

3. A method for liquid dispensing using the liquid dispensing equipment according to any one of claims 1-2, characterized in that, The method includes: pouring the liquid to be dispensed into the dispensing chamber (22), when the dispensing chamber (22) and the return liquid component (23) are sealed, quantitatively dispensing the liquid to be dispensed and storing the quantitatively dispensed liquid, and applying external force to separate the dispensing chamber (22) from the return liquid component (23), and the stored quantitatively dispensed liquid flows down along the outer wall of the return liquid component (23) and flows to the pipette plate (4).

4. The application of the liquid dispensing device according to any one of claims 1-2 or the liquid dispensing method according to claim 3 in biology and chemistry.

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