Conditioned medium of TERT-overexpressing brain pericytes and its application in protecting neurons from hypoxia-ischemia

By constructing a conditioned culture medium for TERT overexpressing pericytes, increasing FGF2 secretion and reducing APOE4 secretion, the problem of difficulty in effectively protecting hypoxic ischemic neurons in the existing technology is solved, and the effect of significantly reducing neuronal apoptosis and promoting survival is achieved.

CN116240171BActive Publication Date: 2025-04-22THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
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Patent Information

Application Number
CN202211492770.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2022-11-25
Publication Date
2025-04-22
Estimated Expiration
2042-11-25

AI Technical Summary

Technical Problem

The prior art is difficult to effectively protect hypoxic ischemic neurons, resulting in serious consequences of hypoxic ischemic encephalopathy in neonates, such as mental retardation, epilepsy, cerebral palsy or developmental delay.

Method used

By constructing a conditioned culture medium for TERT overexpressing pericytes, the second-generation pericytes were transfected with TERT-containing lentiviruses, increasing the secretion of FGF2 that promotes neuronal survival and reducing the secretion of the neuronal toxic molecule APOE4, thereby protecting hypoxic ischemic neurons.

Benefits of technology

This method significantly reduces the apoptosis induced by hypoxic ischemia, promotes neuronal survival, and provides a new technical solution to protect hypoxic ischemia neurons.

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Abstract

The present invention discloses a TERT-overexpressing pericyte conditioned medium and its application in protecting hypoxic-ischemic neurons. The TERT-overexpressing pericyte conditioned medium of the present invention uses a lentivirus containing TERT to transfect second-generation pericytes to construct pericytes overexpressing TERT; the pericytes overexpressing TERT are then added to a serum-free high-glucose DMEM medium for culture, thereby obtaining a TERT-overexpressing pericyte conditioned medium. The present invention proves through experiments that the TERT-overexpressing pericyte conditioned medium increases the secretion of FGF2 that promotes neuronal survival and reduces the secretion of the neuronal toxic molecule APOE4, thereby protecting hypoxic-ischemic neurons, reducing hypoxia-ischemia-induced neuronal apoptosis, and promoting their survival. Therefore, the TERT-overexpressing pericyte conditioned medium can be used to protect hypoxic-ischemic neurons.
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