A method for high-altitude layering propagation of Aquilaria sinensis germplasm

Abstract

The present invention discloses a method for propagating white aurantia odorifera germplasm by high-altitude layering. The method comprises the following steps: making a girdling cut on a branch, first applying a substrate A containing a rooting agent to the girdling cut, then adding a substrate B and coating and fixing the girdling cut; the rooting agent comprises naphthaleneacetic acid, indoleacetic acid, and trace elements. The method can promote rapid rooting of white aurantia odorifera germplasm, with a rooting rate exceeding 80%, thus overcoming the difficulty of rooting white aurantia odorifera germplasm.

Description

Technical Field

[0001] The invention relates to the technical field of plant propagation, and in particular to a method for high-altitude layering propagation of Aquilaria sinensis germplasm. Background Art

[0002] Aquilaria sinensis (Lour.) Gilg is the only source of domestically produced agarwood listed in the Chinese Pharmacopoeia. Aquilaria sinensis is a species of agarwood. Currently, the Hainan Branch of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, has bred two superior varieties of Aquilaria sinensis, "Qixiang 21" and "Qixiang 31." Compared to common Aquilaria sinensis, Aquilaria sinensis possesses the advantages of being easy to produce agarwood and producing agarwood with a high oil content. However, since seed propagation cannot maintain the excellent agarwood-forming properties of the mother plant, asexual reproduction is the only option.

[0003] Common methods of asexual plant propagation include cuttings, grafting, layering, and tissue culture. In the breeding and production of Aquilaria species, aside from grafting, the other three techniques have not been widely used. High-altitude layering, also known as air layering, is a type of layering propagation. While it has great potential for breeding rare species and cultivating large seedlings, research on related technologies has been slow, and its current scope of application is extremely limited. Due to the difficulty in rooting white agarwood germplasm, high-altitude layering has not yet been successfully tested. Summary of the Invention

[0004] The invention aims to provide a method for high-altitude layering propagation of Aquilaria sinensis germplasm, so as to solve the problem of difficult rooting.

[0005] The scheme of the present invention is:

[0006] A method for high-altitude layering propagation of white aurantium germplasm comprises the following steps: making ring cuts on branches, first adding a matrix A containing a rooting agent to the ring cuts, then adding a matrix B and coating and fixing them; the rooting agent comprises naphthaleneacetic acid, indoleacetic acid and trace elements.

[0007] Preferably, the substrate A is slightly acidic soil with a pH of 5.5-6.5. Slightly acidic conditions of pH 5.5-6.5 can significantly increase the rooting rate.

[0008] Preferably, the mass ratio of the rooting agent to the slightly acidic soil is 1:10-20.

[0009] Preferably, the trace elements include MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O 24 .

[0010] Preferably, the trace elements include MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.2-0.6:2-3:2-5:0.2-0.4. 24 .

[0011] Preferably, the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 1-3:2-5:5.

[0012] Preferably, the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5.

[0013] Preferably, the branches are branches with a diameter of 1.5 cm or more.

[0014] Preferably, the matrix B is wood chips, perlite and peat soil in a mass ratio of 3:1:5.

[0015] Preferably, the white agarwood germplasm includes Qixiang No. 21 and Qixiang No. 31.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The present invention involves girdling branches of aloesia sinensis germplasm to obtain a girdling cut, wrapping the cut with a substrate A containing a rooting agent, and adding a loose, breathable substrate B. This allows the aloesia sinensis germplasm to begin taking root within four weeks, exhibiting rapid rooting and a robust root system, with a rooting rate exceeding 80% and a transplant survival rate exceeding 90%. This method can effectively improve the propagation efficiency of aloesia sinensis germplasm through aerial layering.

[0018] The present invention screens out the optimal growth hormone and trace element components and ratios. When the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5, the rooting rate can reach more than 90%. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 : Example 1 White Aquilaria sinensis germplasm 2 months induced rooting diagram

[0020] Figure 2 : Example 1 White Aquilaria sinensis germplasm 4 months induced rooting diagram DETAILED DESCRIPTION

[0021] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0022] Example 1:

[0023] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut surface;

[0024] The rooting agent comprises naphthylacetic acid, indoleacetic acid and trace elements, wherein the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5. The mass ratio of the rooting agent to the slightly acidic soil is 1:10.

[0025] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0026] Twenty Aquilaria sinensis plants were selected, and one branch from each plant was subjected to the above treatment. This process was repeated three times. The rooting rate was calculated two months after treatment, and the transplant survival rate was calculated one month after the rooted seedlings were transplanted. The results showed that the above treatment method enabled rapid rooting and a strong root system, with a rooting rate (rooting rate = number of roots / total number of treatments × 100%) reaching 90%, and a transplant survival rate (transplant survival rate = number of transplant survivals / total number of transplants × 100%) reaching 95%.

[0027] Example 2:

[0028] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut surface;

[0029] The rooting agent comprises naphthylacetic acid, indoleacetic acid and trace elements, wherein the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5. The mass ratio of the rooting agent to the slightly acidic soil is 1:20.

[0030] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0031] The results showed that after treatment with the above method, the plants were able to take root quickly and the root system was strong, with a rooting rate of 88% and a transplanting survival rate of 95%.

[0032] Example 3:

[0033] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.5), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the surface;

[0034] The rooting agent comprises naphthylacetic acid, indoleacetic acid and trace elements, wherein the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5. The mass ratio of the rooting agent to slightly acidic soil is 1:10.

[0035] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:3:5:0.2 24 .

[0036] The results showed that after treatment with the above method, the plants were able to take root quickly and the root system was strong, with a rooting rate of 90% and a transplanting survival rate of 93%.

[0037] Example 4:

[0038] The method for propagating agarwood germplasm by aerial layering comprises the following steps: making a ring cut on a branch with a diameter of 1.5 cm or more (the size of the incision is 1.5 times the diameter of the branch), first applying a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 5.5), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the surface;

[0039] The rooting agent includes naphthaleneacetic acid, indoleacetic acid and trace elements in a mass ratio of 2:3:5. The mass ratio of the rooting agent to slightly acidic soil is 1:10.

[0040] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.2:3:5:0.4 24 .

[0041] The results showed that after treatment with the above method, the plants were able to take root quickly and the root system was strong, with a rooting rate of 86% and a transplanting survival rate of 93%.

[0042] Example 5:

[0043] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut surface;

[0044] The rooting agent includes naphthaleneacetic acid, indoleacetic acid and trace elements in a mass ratio of 1:3:5. The mass ratio of the rooting agent to slightly acidic soil is 1:10.

[0045] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0046] The results showed that after treatment with the above method, the plants were able to take root quickly and the root system was strong, with a rooting rate of 89% and a transplant survival rate of 93%.

[0047] Example 6:

[0048] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first applying a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and sandy peat soil in a mass ratio of 3:1:5), and then coating and fixing the ring cut surface with a film;

[0049] The rooting agent includes naphthaleneacetic acid, indoleacetic acid and trace elements, with the mass ratio of naphthaleneacetic acid, indoleacetic acid and trace elements being 1:5:5. The mass ratio of the rooting agent to slightly acidic soil is 1:10.

[0050] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0051] The results showed that after treatment with the above method, the plants were able to take root quickly and the root system was strong, with a rooting rate of 90% and a transplant survival rate of 92%.

[0052] Example 7:

[0053] The method for propagating agarwood germplasm by aerial layering comprises the following steps: making a ring cut on a branch with a diameter of 1.5 cm or more (the size of the incision is 1.5 times the diameter of the branch), first applying a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.1), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the surface;

[0054] The rooting agent comprises naphthylacetic acid, indoleacetic acid and trace elements, wherein the mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:5. The mass ratio of the rooting agent to the slightly acidic soil is 1:10.

[0055] The trace elements are CuSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0056] The results showed that after treatment with the above method, the plant can take root quickly and the root system is strong, with a rooting rate of 80% and a transplant survival rate of 98%.

[0057] Example 8:

[0058] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut surface;

[0059] The rooting agent includes indoleacetic acid and trace elements, with the mass ratio of indoleacetic acid to trace elements being 3:5. The mass ratio of the rooting agent to slightly acidic soil is 1:10.

[0060] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0061] After treatment with the above method, the rooting rate was 53% and the transplanting survival rate was 86%.

[0062] Example 9

[0063] The method for aerial layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of more than 1.5 cm (the incision size is 1.5 times the branch diameter), first adding a rooting agent to the ring cut surface with a substrate A (i.e., slightly acidic soil with a pH of 6.0), and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut surface;

[0064] The rooting agent includes naphthaleneacetic acid and trace elements, and the mass ratio of naphthaleneacetic acid to trace elements is 2:5.

[0065] The mass ratio of the rooting agent to the slightly acidic soil is 1:10.

[0066] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0067] The results showed that after treatment with the above method, the rooting rate was 60% and the transplanting survival rate was 86%.

[0068] Example 10

[0069] A method for high-altitude layering propagation of white agarwood germplasm comprises the following steps: making a ring cut on a branch with a diameter of 1.5 cm or more (the incision size is 1.5 times the branch diameter), first adding a substrate A (i.e., neutral soil with a pH of 7.2) containing a rooting agent to the ring cut, and then adding a substrate B (i.e., sawdust, perlite, and peat soil in a mass ratio of 3:1:5) and coating and fixing the ring cut;

[0070] The rooting agent includes naphthaleneacetic acid, indoleacetic acid and trace elements, with the mass ratio of naphthaleneacetic acid, indoleacetic acid and trace elements being 2:3:5. The mass ratio of the rooting agent to neutral soil is 1:10.

[0071] Trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.6:2:2:0.2 24 .

[0072] After treatment with the above method, the rooting rate was 74% and the transplanting survival rate was 81%.

[0073] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A method for high-altitude layering propagation of Aquilaria sinensis germplasm, characterized in that: The method comprises the following steps: making a ring cut on a branch, first adding a matrix A containing a rooting agent to the ring cut, then adding a matrix B and coating and fixing the ring cut; the rooting agent comprises naphthaleneacetic acid, indoleacetic acid and trace elements; the matrix A is slightly acidic soil with a pH of 5.5-6.5; the trace elements are MnSO4, H3BO3, ZnSO4, EDTA-2Na and (NH4)6Mo7O in a mass ratio of 1:0.2-0.6:2-3:2-5:0.2-0.

4. 24 ; The matrix B is wood chips, perlite and peat soil in a mass ratio of 3:1:

5.

2. The method for high-altitude layering propagation of the Aquilaria sinensis germplasm according to claim 1, characterized in that: The mass ratio of the rooting agent to the slightly acidic soil is 1:10-20.

3. The method for high-altitude layering propagation of the germplasm of Aquilaria sinensis according to claim 1, characterized in that: The mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 1~3:2~5:

5.

4. The method for high-altitude layering propagation of the germplasm of Aquilaria sinensis according to claim 1, characterized in that: The mass ratio of naphthylacetic acid, indoleacetic acid and trace elements is 2:3:

5.

5. The method for high-altitude layering propagation of the germplasm of Aquilaria sinensis according to claim 1, characterized in that: The branches are branches with a diameter of more than 1.5 cm.

6. The method for high-altitude layering propagation of the germplasm of Aquilaria sinensis according to claim 1, characterized in that: The matrix B is wood chips, perlite and peat soil in a mass ratio of 3:1:

5.

7. The method for high-altitude layering propagation of the germplasm of Aquilaria sinensis according to claim 1, characterized in that: The white agarwood germplasm includes Qixiang No. 21 and Qixiang No. 31.

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