A lactating sow reproduction promoting feed additive, and a preparation method and application thereof

Abstract

The present application relates to a lactating sow promoting reproduction feed additive and its preparation method and application, the component of the lactating sow promoting reproduction feed additive includes: fermented Chinese herbal medicine compound, carbohydrate, rutin and carrier material;The fermented Chinese herbal medicine compound is the product obtained by fermentation of Chinese herbal medicine fermentation substrate by complex bacteria and complex enzyme;The Chinese herbal medicine fermentation substrate includes the combination of salvia, radix puerariae, radix bupleuri, hibiscus syriacus and quercus.The present application develops a feed additive according to the physiological characteristics and nutritional requirements of lactating sows, and the active nutrients cooperate with each other, which can not only improve the milk yield and quality, but also promote follicular development in sows, shorten the number of days from weaning to estrus in sows, and improve the subsequent reproductive performance.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of pig breeding, and relates to a lactating sow reproduction-promoting feed additive as well as a preparation method and application thereof, in particular to a lactating sow reproduction-promoting feed additive for improving milk yield and milk quality as well as a preparation method and application thereof. BACKGROUND

[0002] The nutrient intake of sows during the lactation period not only affects the milk yield and quality, but also seriously affects the subsequent reproductive performance. However, during the lactation process of sows, the physiological state and nutrient metabolism of the body change significantly, which induces the body to produce excessive free radicals, resulting in oxidative stress. Oxidative stress can lead to endocrine function disorder in the body, and insufficient intake of functional nutrients by sows, thereby leading to insufficient lactation, poor milk quality, long weaning estrus interval, and serious harm to the health of sows and the subsequent reproductive performance.

[0003] At present, in the actual production, the quality of feed is often improved and the oxidative stress of lactating sows is reduced by directly adding antioxidants. For example, CN103931891A discloses a lactating sow health-care premix which is made of the following raw material components in parts by weight: lactating sow multi-mineral 21 parts, phytase 1.5 parts, lactating sow multi-vitamin 2 parts, ascorbic acid 1.5 parts, special enzyme 1.5 parts, probiotic 1.5 parts, organic chromium 2.5 parts, sweetener 2 parts, antioxidant 1 part, skin redness 2.5 parts, bacitracin zinc 2.5 parts, choline chloride 7 parts, and the like.

[0004] However, there are various types of antioxidants on the market, and the components are complex and diverse, and the antioxidants have certain toxic side effects, which leads to the failure to fully alleviate the oxidative stress of lactating sows. Therefore, it is very necessary to develop a green and safe product which can effectively alleviate the oxidative stress of lactating sows, improve the milk yield and quality, and improve the subsequent reproductive performance. SUMMARY

[0005] In view of the deficiencies of the prior art, the present application aims to provide a lactating sow reproduction-promoting feed additive as well as a preparation method and application thereof, in particular to provide a lactating sow reproduction-promoting feed additive for improving milk yield and milk quality as well as a preparation method and application thereof.

[0006] To achieve the object of the present application, the following technical solutions are adopted:

[0007] In a first aspect, the present application provides a lactating sow reproduction-promoting feed additive, and the components of the lactating sow reproduction-promoting feed additive comprise: a fermented Chinese herbal medicine compound, a carbohydrate, rutin, and a carrier material.

[0008] The fermented Chinese herbal medicine complex is a product obtained by fermenting a Chinese herbal medicine fermentation substrate with a composite bacteria and a composite enzyme; the Chinese herbal medicine fermentation substrate comprises a combination of sage, kudzu root, bupleurum, roselle and quercetin.

[0009] The present invention develops a feed additive based on the physiological characteristics and nutritional needs of lactating sows. The three functional components of fermented Chinese herbal medicine complex, carbohydrates and rutin are compounded. The rich active nutrients cooperate with each other, which can not only improve the production and quality of breast milk, but also promote the development of sow follicles, shorten the time from weaning to estrus, and improve the subsequent reproductive performance of sows.

[0010] Among them, the fermented Chinese herbal complex is made by compounding five specific Chinese herbal components as fermentation substrates. The Chinese herbal components are scientifically combined and synergistically enhanced, so that the fermentation product has significantly improved immunity-enhancing, antioxidant, and antiviral effects. It can not only improve the oxidative stress of lactating sows, improve the body's antioxidant capacity, and increase breast milk production and quality, but also inhibit the excessive accumulation of HCY (homocysteine), alleviate the toxic effects of HCY on follicular development, improve oocyte quality, and improve reproductive performance.

[0011] Among them, rutin can relieve stress stimulation of sows, and can also inhibit DNA methylation of mammary epithelial cells to avoid increased levels of DNA methylation in mammary epithelial cells, promote the synthesis and secretion of milk fat and lactose in mammary epithelial cells, thereby increasing sow milk production and quality.

[0012] Because lactose is the osmotic pressure regulator of breast milk, the yield and rate of its synthesis directly impact sow milk production. The carbohydrates in the feed additive described herein provide a continuous feedstock for lactose synthesis, thereby increasing lactose production and, in turn, improving breast milk yield and quality. Lactose is also the only sugar in breast milk that can be directly absorbed by piglets, providing them with the carbohydrates they need for growth.

[0013] In addition, since the effective ingredients of Chinese herbal medicines are mostly distributed in cells and intercellular spaces, and the cell walls are mainly composed of macromolecules such as cellulose, hemicellulose, and pectin, the fermented Chinese herbal medicine complex used in the present invention is a product obtained by fermenting Chinese herbal medicine fermentation substrates in a coordinated manner by composite bacteria and composite enzymes. It can effectively digest plant cell walls, release active substances, and produce new enzyme and bacterial active substances, thereby exerting a better effect.

[0014] In summary, the feed additive involved in the present invention can effectively alleviate the oxidative stress of lactating sows, and solve the current problems of lactating sows such as low milk production, poor milk quality, anestrus, long weaning-estrus interval, and poor reproductive performance.

[0015] Preferably, the Chinese herbal fermentation substrate comprises, by weight, a combination of 5-10 parts of sage, 1-5 parts of kudzu root, 1-10 parts of bupleurum, 1-5 parts of roselle and 1-10 parts of quercetin.

[0016] The present invention also creatively discovered that when the five Chinese herbal ingredients are compounded in the above-mentioned specific mass ratio, the fermentation product can have better effects in improving the oxidative stress of lactating sows, enhancing the body's antioxidant capacity, increasing breast milk production and quality, improving oocyte quality, and improving reproductive performance.

[0017] The mass fraction of the sage can be selected from 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the kudzu root can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.; the mass fraction of the bupleurum can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the roselle can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.; the mass fraction of the quercetin can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.

[0018] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0019] Preferably, the composite bacteria is a combination of Aspergillus oryzae, Lactobacillus and Bacillus subtilis.

[0020] The fermented herbal compound used in the present invention is preferably fermented with a combination of Aspergillus oryzae, Lactobacillus and Bacillus subtilis to provide a fermentation product with superior antioxidant capacity. Furthermore, the combined fermentation is performed in the following specific mass ratios to maximize the effect.

[0021] Preferably, the ratio of the viable counts of Aspergillus oryzae, Lactobacillus and Bacillus subtilis is 1:(2-4):(2-3), for example, 1:2:2, 1:2:3, 1:3:2, 1:3:3, 1:4:2, 1:4:3, etc.; the total viable count of the composite bacteria is greater than 1×10 9 CFU / g, for example 1×10 9 CFU / g, 5×10 9 CFU / g, 1×10 10 CFU / g, 5×10 10 CFU / g, 1×10 11 CFU / g, etc.

[0022] Preferably, the dosage of the composite bacteria is 1-5% of the mass of the Chinese herbal medicine fermentation substrate, such as 1%, 2%, 3%, 4%, 5%, etc.

[0023] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0024] Preferably, the complex enzyme is a combination of cellulase, xylanase and tannase.

[0025] The fermented herbal complex used in the present invention is preferably fermented with a combination of cellulase, xylanase, and tannase to provide a product with superior antioxidant capacity. Furthermore, the combined fermentation is performed in the following specific mass ratios to maximize the effect.

[0026] Preferably, the mass ratio of the cellulase, xylanase and tannase is (3-4):(1-3):1, for example, 3:1:1, 3:2:1, 3:3:1, 4:1:1, 4:2:1, 4:3:1, etc.

[0027] Preferably, the dosage of the complex enzyme is 0.5-2% of the mass of the Chinese herbal medicine fermentation substrate, for example, 0.5%, 0.8%, 1%, 1.2%, 1.5%, 1.7%, 2%, etc.

[0028] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0029] In the present invention, the fermented Chinese herbal medicine complex is prepared by a preparation method comprising the following steps:

[0030] (1) Mix the various Chinese herbal fermentation substrates, crush them, sieve them, and set them aside; mix the composite bacteria with 30-40°C water for oxygen activation, and then mix them with the composite enzyme and set them aside;

[0031] (2) mixing the obtained bacterial enzyme liquid with the treated fermentation substrate, performing anaerobic fermentation, and drying to obtain the fermented Chinese herbal medicine complex.

[0032] Preferably, the oxygen supply activation treatment time is 3-5 hours, for example, 3 hours, 3.2 hours, 3.5 hours, 4 hours, 4.2 hours, 4.5 hours, 4.8 hours, 5 hours, etc.

[0033] Preferably, the temperature of the anaerobic fermentation is 15-40° C., for example, 15° C., 20° C., 25° C., 30° C., 35° C., 40° C., etc.; the time of the anaerobic fermentation is 3-7 days, for example, 3 days, 4 days, 5 days, 6 days, 7 days, etc. Other specific values ​​within the above numerical ranges can be selected and will not be detailed here.

[0034] The preparation process of the fermented Chinese herbal medicine complex of the present invention is simple and easy to operate, and is suitable for industrial large-scale production.

[0035] In the present invention, the carbohydrate is a combination of fast-fermenting carbohydrates and slow-fermenting carbohydrates.

[0036] The carbohydrates in the feed additive involved in the present invention are prepared in proportion from fast-fermenting carbohydrates and slow-fermenting carbohydrates according to their fermentation speeds in the sow's digestive tract and the synthesis mechanism of lactose. This can more effectively and continuously provide raw materials for the synthesis of lactose, thereby increasing lactose production and further improving breast milk production and quality.

[0037] Preferably, the mass ratio of the rapidly fermentable carbohydrates to the slowly fermentable carbohydrates is (2-4):(2-3), for example, 1:1, 3:2, 2:1, 2:3, 4:3, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0038] Preferably, the rapidly fermentable carbohydrates are derived from beta-yeast glucan and manno-oligosaccharides.

[0039] Preferably, the slowly fermentable carbohydrates are derived from fig peels and pea peels.

[0040] Preferably, the components of the lactating sow reproduction-promoting feed additive include, by mass, 200-500 parts of a fermented Chinese herbal compound, 50-200 parts of carbohydrates, 1-10 parts of rutin, and 200-800 parts of a carrier material.

[0041] The mass fraction of the fermented Chinese herbal medicine complex can be selected from 200 parts, 220 parts, 250 parts, 280 parts, 300 parts, 350 parts, 400 parts, 450 parts, 500 parts, etc.; the mass fraction of the carbohydrate can be selected from 50 parts, 80 parts, 100 parts, 120 parts, 140 parts, 150 parts, 170 parts, 180 parts, 200 parts, etc.; the mass fraction of the rutin can be selected from 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.; the mass fraction of the carrier material can be selected from 200 parts, 300 parts, 350 parts, 400 parts, 450 parts, 500 parts, 600 parts, 700 parts, 800 parts, etc.

[0042] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0043] Preferably, the carrier material comprises corn gluten meal.

[0044] In a second aspect, the present invention provides a method for preparing the lactating sow reproduction-promoting feed additive according to the first aspect, the preparation method comprising: uniformly mixing the fermented Chinese herbal medicine complex, carbohydrates, rutin and a carrier material.

[0045] In a third aspect, the present invention provides a lactating sow reproduction promoting feed, comprising a basal diet and the lactating sow reproduction promoting feed additive according to the first aspect, wherein the lactating sow reproduction promoting feed additive accounts for 0.5%-2% of the mass of the basal diet, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.2%, 1.5%, 2%, etc. Other specific point values ​​within this numerical range can be selected and will not be repeated here.

[0046] Compared with the prior art, the present invention has the following beneficial effects:

[0047] The present invention develops a feed additive based on the physiological characteristics and nutritional needs of lactating sows. The three functional components of fermented Chinese herbal medicine complex, carbohydrates and rutin are compounded. The rich active nutrients cooperate with each other, which can not only improve the production and quality of breast milk, but also promote the development of sow follicles, shorten the time from weaning to estrus, and improve the subsequent reproductive performance of sows.

[0048] Among them, the fermented Chinese herbal complex is made by compounding five specific Chinese herbal components as fermentation substrates. The Chinese herbal components are scientifically combined and synergistically enhanced, so that the fermentation product has significantly improved immunity-enhancing, antioxidant, and antiviral effects. It can not only improve the oxidative stress of lactating sows, improve the body's antioxidant capacity, and increase breast milk production and quality, but also inhibit the excessive accumulation of HCY (homocysteine), alleviate the toxic effects of HCY on follicular development, improve oocyte quality, and improve reproductive performance.

[0049] Rutin can alleviate stress in sows and inhibit DNA methylation in mammary epithelial cells, thereby preventing increased DNA methylation levels. It can also promote the synthesis and secretion of milk fat and lactose in mammary epithelial cells, thereby improving sow milk production and quality. Carbohydrates can continuously provide raw materials for lactose synthesis, thereby increasing lactose production and, in turn, improving breast milk production and quality.

[0050] In summary, the feed additive involved in the present invention can effectively alleviate the oxidative stress of lactating sows, and solve the current problems of lactating sows such as low milk production, poor milk quality, anestrus, long weaning-estrus interval, and poor reproductive performance. DETAILED DESCRIPTION

[0051] In order to further illustrate the technical means and effects adopted by the present invention, the technical solutions of the present invention are further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0052] The Aspergillus oryzae powder involved in the following is purchased from Jining Zechuan Biotechnology Co., Ltd., with a viable count of 1.5×10 10CFU / g product; Bacillus subtilis powder was purchased from Shandong Zhongke Jiayi Bioengineering Co., Ltd. with a viable count of 1×10 11 CFU / g of the product; Lactobacillus powder was purchased from Shaanxi Chenming Biotechnology Co., Ltd. with a viable count of 1×10 11 CFU / g of product;

[0053] Xylanase was purchased from Shaanxi Rankang Biotechnology Co., Ltd. with an enzyme activity of 52561 U / g;

[0054] Cellulase was purchased from Shaanxi Rankang Biotechnology Co., Ltd. with an enzyme activity of 112842 U / g;

[0055] Tanninase was purchased from Guangzhou Huayu Biotechnology Co., Ltd. with an enzyme activity of 300 U / g;

[0056] Rutin was purchased from Xi'an Youshuo Biotechnology Co., Ltd. with a specification of 95%;

[0057] β-yeast glucan was purchased from Nanjing Taixin Biotechnology Co., Ltd. and has a soluble β-yeast glucan content of 80%;

[0058] Mannan oligosaccharide was purchased from Shandong Hailongyuan Biotechnology Co., Ltd. and had a mannan oligosaccharide content of 90%;

[0059] Corn gluten meal was purchased from Shandong Yunze Biotechnology Co., Ltd. with a model number of 3540.

[0060] Preparation Example 1-1

[0061] This preparation example provides a fermented Chinese herbal medicine complex, and the preparation method thereof is as follows:

[0062] (1) Sage, Pueraria root, Bupleurum root, Roselle flower and Quercus bark were mixed in a mass ratio of 2:1:1:1:1, and passed through a 40-mesh sieve to prepare a fermentation substrate;

[0063] (2) Mix Aspergillus oryzae powder, Lactobacillus powder and Bacillus subtilis powder (total viable bacteria count greater than 1×10 10 After mixing, add 5 times the mass of 35℃ warm water, stir evenly, activate with oxygen supply for 4 hours, and then mix with cellulase, xylanase and tannase in a mass ratio of 3:2:1, where the mass ratio of total bacterial powder to total enzyme is 3:1, and use it as bacterial enzyme solution for standby use;

[0064] (3) The bacterial enzyme solution was mixed with an equal mass of water and then added to the fermentation substrate, wherein the amount of the composite bacteria was 3% of the mass of the fermentation substrate, and the amount of the composite enzyme was 1% of the mass of the fermentation substrate. The mixture was stirred evenly, sealed and bagged, and solid-state anaerobic fermentation was performed at 30°C for 5 days. The mixture was dried after fermentation to obtain a fermented Chinese herbal medicine complex.

[0065] Preparation Example 1-2

[0066] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the fermentation substrate is different, namely, a mixture of Pueraria root, Bupleurum root, Roselle flower and Quercus bark in a mass ratio of 1:1:1:1, which is passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0067] Preparation Examples 1-3

[0068] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the fermentation substrate is different, which is a mixture of sage, bupleurum, roselle and quercetin in a mass ratio of 2:1:1:1, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0069] Preparation Examples 1-4

[0070] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the fermentation substrate is different, which is a mixture of sage, kudzu root, roselle and quercetin in a mass ratio of 2:1:1:1, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0071] Preparation Examples 1-5

[0072] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the fermentation substrate is different, namely, a mixture of sage, kudzu root, bupleurum and quercetin in a mass ratio of 2:1:1:1, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0073] Preparation Examples 1-6

[0074] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the fermentation substrate is different, which is a mixture of sage, kudzu root, bupleurum, and roselle in a mass ratio of 2:1:1:1, passed through a 40-mesh sieve, and the amount of the fermentation substrate remains unchanged.

[0075] Preparation Examples 1-7

[0076] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the complex enzyme is replaced with xylanase and tannase in a mass ratio of 2:1, but the amount of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0077] Preparation Examples 1-8

[0078] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the complex enzyme is replaced with cellulase and tannase in a mass ratio of 3:1, but the amount of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0079] Preparation Examples 1-9

[0080] This preparation example provides a fermented Chinese herbal medicine complex. The preparation method thereof differs from that of Preparation Example 1 only in that the complex enzyme is replaced with cellulase and xylanase in a mass ratio of 3:2, but the amount of the complex enzyme is still 1% of the mass of the fermentation substrate, and other conditions remain unchanged.

[0081] Preparation Example 2-1

[0082] This preparation example provides a carbohydrate raw material, and its preparation method is as follows:

[0083] The fig peel and pea peel are respectively crushed into 80 meshes, and then 10 parts of β-yeast glucan, 10 parts of manno-oligosaccharide, 5 parts of fig peel and 5 parts of pea peel are evenly mixed to obtain the product.

[0084] Preparation Example 2-2

[0085] This preparation example provides a carbohydrate raw material, and its preparation method is as follows:

[0086] Mix 15 parts of β-yeast glucan and 15 parts of mannooligosaccharide evenly to obtain the product.

[0087] Preparation Example 2-3

[0088] This preparation example provides a carbohydrate raw material, and its preparation method is as follows:

[0089] Crush fig peel and pea peel into 80 mesh respectively, then mix 15 parts of fig peel and 15 parts of pea peel evenly to obtain the product.

[0090] Example 1

[0091] This example provides a lactating sow reproduction-promoting feed additive, comprising, by weight, 300 parts of the fermented Chinese herbal compound prepared in Preparation Example 1-1, 100 parts of the carbohydrate prepared in Preparation Example 2-1, 10 parts of rutin, and 590 parts of corn gluten meal. The feed additive is prepared by uniformly mixing the fermented Chinese herbal compound, carbohydrate, rutin, and corn gluten meal.

[0092] Example 2

[0093] The embodiment provides a lactating sow reproduction promoting feed additive, which is composed of 350 parts of the fermented Chinese herbal compound prepared in Preparation Example 1-1, 50 parts of the carbohydrate prepared in Preparation Example 2-1, 10 parts of rutin and 590 parts of corn gluten meal in terms of mass fraction. The fermented Chinese herbal compound, the carbohydrate, the rutin and the corn gluten meal are uniformly mixed to obtain the lactating sow reproduction promoting feed additive.

[0094] Example 3-7

[0095] The embodiment provides five kinds of lactating sow reproduction promoting feed additives, which are different from the lactating sow reproduction promoting feed additive of Example 1 only in that the fermented Chinese herbal compound prepared in Preparation Example 1-1 is replaced by the fermented Chinese herbal compound prepared in Preparation Example 1-2, Preparation Example 1-3, Preparation Example 1-4, Preparation Example 1-5 and Preparation Example 1-6 respectively in equal amount, and other components and contents remain unchanged.

[0096] Example 8-10

[0097] The embodiment provides three kinds of lactating sow reproduction promoting feed additives, which are different from the lactating sow reproduction promoting feed additive of Example 1 only in that the fermented Chinese herbal compound prepared in Preparation Example 1-1 is replaced by the fermented Chinese herbal compound prepared in Preparation Example 1-7, Preparation Example 1-8 and Preparation Example 1-9 respectively in equal amount, and other components and contents remain unchanged.

[0098] Example 11-12

[0099] The embodiment provides two kinds of lactating sow reproduction promoting feed additives, which are different from the lactating sow reproduction promoting feed additive of Example 1 only in that the carbohydrate prepared in Preparation Example 2-1 is replaced by the carbohydrate prepared in Preparation Example 2-2 and Preparation Example 2-3 respectively in equal amount, and other components and contents remain unchanged.

[0100] Comparative Example 1

[0101] The comparative example provides a lactating sow reproduction promoting feed additive, which is different from the lactating sow reproduction promoting feed additive of Example 1 only in that the rutin is not contained, and the reduced amount of the rutin is distributed on the mass of the fermented Chinese herbal compound, and other components and contents remain unchanged.

[0102] Test Example

[0103] A single factor test design is adopted, 126 two-year-old sows are selected, and are divided into 14 treatment groups under the premise that the number of piglets in each group is consistent, wherein 1 control group and 13 experimental groups, the control group is fed with a basic diet (corn 56%, soybean meal 27%, fish meal 3%, bran 10%, premix 4%, the premix is purchased from Guangzhou Aonong Biotechnology Co., Ltd.), and the experimental groups are respectively added with 1% of the additives prepared in Example 1-12 and Comparative Example 1 on the basis of the basic diet, three repetitions are set for each treatment, three sows are set for each repetition, the test period is from the parturition of the sows to the weaning estrus, and the piglets are weaned at 21 days.

[0104] (1) On day 7 after farrowing, blood was collected from the sows' ear veins. The blood samples were allowed to stand at 25°C for 10 min and centrifuged at 3000 rpm for 15 min to prepare serum. The serum was transferred to Eppendorf tubes and stored at -20°C. The HCY content in the serum was determined using an ELISA kit purchased from Nanjing Jiancheng Biotechnology Co., Ltd., and the specific procedures were performed according to the manufacturer's instructions. The HCY content results for each group are shown in Table 1.

[0105] Table 1

[0106] Group HCY content (μmol / L) Example 1 14.68 Example 2 14.83 Example 3 18.74 Example 4 17.52 Example 5 17.13 Example 6 17.85 Example 7 18.06 Example 8 17.67 Example 9 17.21 Example 10 17.03 Example 11 15.32 Example 12 14.97 Comparative Example 1 15.06 control group 21.92

[0107] The results in Table 1 show that the feed additive of the present invention has the effect of significantly inhibiting the excessive accumulation of HCY and alleviating the toxic effect of HCY on follicular development, and the fermented herbal compound plays a major contribution, wherein the formula and preparation process of the fermented herbal compound affect this effect.

[0108] (2) On day 7 after farrowing, approximately 30 mL of milk was collected from each sow and stored at -20°C. Milk fat, milk protein, and lactose were measured using an automated analyzer. IgG levels were analyzed by colorimetry using an automated biochemical analyzer. The results are shown in Table 2.

[0109] Table 2

[0110] Group lactose(%) Milk protein (%) Milk fat (%) IgG (g / L) Example 1 7.12 4.38 5.52 49.65 Example 2 6.87 4.20 5.13 48.83 Example 3 5.85 3.82 4.78 47.79 Example 4 5.66 3.76 4.43 47.02 Example 5 5.73 3.80 4.56 46.62 Example 6 6.01 3.96 4.87 46.83 Example 7 6.12 3.85 4.76 46.53 Example 8 6.21 3.78 4.89 48.02 Example 9 6.15 4.01 5.02 48.24 Example 10 6.06 3.96 4.98 47.87 Example 11 5.88 3.87 4.68 48.32 Example 12 5.72 3.96 4.87 48.53 Comparative Example 1 5.53 3.68 4.65 46.57 control group 5.36 3.52 4.43 44.56

[0111] The results in Table 2 show that the feed additive of the present invention has the effect of significantly increasing the levels of lactose, milk protein, milk fat, and IgG in sow milk, and can significantly improve the quality of sow milk. The formula and preparation process of the fermented Chinese herbal compound and the carbohydrate formula all have a certain influence on this effect.

[0112] (3) On the 7th day after weaning, the weight loss of sows, piglet survival rate and piglet weight gain of each group were counted. The results are shown in Table 3.

[0113] Table 3

[0114]

[0115] The results in Table 3 show that the feed additive of the present invention has the effect of significantly improving the survival rate and weight gain of piglets, reducing the weight loss of lactating sows, and significantly improving the reproductive performance of sows. The formula and preparation process of the fermented Chinese herbal compound and the carbohydrate formula all have a certain influence on this effect.

[0116] The applicant declares that the present invention uses the above-mentioned embodiments to illustrate the lactating sow reproduction-promoting feed additive, its preparation method, and its application, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent replacements for the raw materials of the product of the present invention, the addition of auxiliary ingredients, the selection of specific methods, etc., all fall within the scope of protection and disclosure of the present invention.

[0117] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the scope of protection of the present invention.

[0118] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any appropriate manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A lactating sow reproduction promoting feed additive, characterized in that: The components of the lactating sow reproduction-promoting feed additive are as follows: 200-500 parts by mass of a fermented Chinese herbal medicine compound, 50-200 parts by mass of carbohydrates, 1-10 parts by mass of rutin, and 200-800 parts by mass of a carrier material; The fermented Chinese herbal medicine complex is a product obtained by fermenting a Chinese herbal medicine fermentation substrate with a composite bacteria and a composite enzyme; the Chinese herbal medicine fermentation substrate is a combination of 5-10 parts by weight of sage, 1-5 parts of kudzu root, 1-10 parts of bupleurum, 1-5 parts of roselle and 1-10 parts of quercetin; the composite bacteria is a combination of Aspergillus oryzae, Lactobacillus and Bacillus subtilis in a ratio of 1:(2-4):(2-3) of viable bacteria, and the total viable bacteria count of the composite bacteria is greater than 1×10 9 CFU / g; the complex enzyme is a combination of cellulase, xylanase and tannase in a mass ratio of (3-4): (1-3): 1; The dosage of the composite bacteria is 1-5% of the mass of the Chinese herbal medicine fermentation substrate, and the dosage of the composite enzyme is 0.5-2% of the mass of the Chinese herbal medicine fermentation substrate; The fermented Chinese herbal medicine complex is prepared by the following steps: (1) Mix the various Chinese herbal fermentation substrates, crush them, sieve them, and set them aside; mix the composite bacteria with 30-40°C water and perform oxygen activation treatment for 3-5 hours, then mix them with the composite enzyme and set them aside; (2) mixing the obtained bacterial enzyme liquid with the treated fermentation substrate, performing anaerobically fermentation at 15-40° C. for 3-7 days, and drying to obtain the fermented Chinese herbal medicine complex; The carbohydrates are beta-yeast glucan, manno-oligosaccharide, fig peel and pea peel.

2. The lactating sow reproduction promoting feed additive according to claim 1, characterized in that The carrier material includes corn gluten meal.

3. The method for preparing the lactating sow reproduction promoting feed additive according to any one of claims 1-2, characterized in that: The preparation method comprises: uniformly mixing the fermented Chinese herbal medicine complex, carbohydrates, rutin and a carrier material.

4. A lactating sow reproduction promoting feed, characterized in that: The lactating sow reproduction promoting feed contains a basic diet and the lactating sow reproduction promoting feed additive according to any one of claims 1 to 2, wherein the lactating sow reproduction promoting feed additive accounts for 0.5% to 2% of the mass of the basic diet.

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