A rhizoma polygonati sprout and a cultivating method thereof

Abstract

The application discloses a kind of Huangjing sprout cultivation methods, comprising the following steps: parent pretreatment;Sprout induction culture;Sprout culture;Parent includes conventional root rhizome, aseptic rhizome and Huangjing seed;Sprout induction culture includes conventional water culture, double-layer water culture and sand bed germination;Sprout culture includes spraying nutrient solution and nutrient solution water planting, and the culture temperature of sprout culture is 4-25 ℃.The application is obtained by sequentially carrying out parent pretreatment, sprout induction culture, sprout culture, Huangjing sprout, by pretreatment to parent, by sprout induction culture and sprout culture, on the one hand, Huangjing's sprouting rate can be improved, on the other hand, Huangjing sprout's stem length and stem thickness can be improved, and then the edibility of Huangjing sprout is improved;Through the cultivation method, the quality and edibility of Huangjing sprout are effectively improved, the cultivation time of Huangjing sprout is effectively shortened, and the promotion of Huangjing sprout is beneficial.

Description

TECHNICAL FIELD

[0001] The present application relates to a Polygonatum sibiricum sprout and a cultivation method thereof, and belongs to the technical field of Polygonatum sibiricum cultivation. BACKGROUND

[0002] Polygonatum sibiricum is a perennial herb of the genus Polygonatum in the family Asparagaceae. In the 2020 edition of Chinese Pharmacopoeia, the medicinal Polygonatum sibiricum is the dried rhizome of Polygonatum kingianum Coll. et Hemsl, Polygonatum sibiricum Red, or Polygonatum cyrtonema Hua. Polygonatum sibiricum has both medicinal and dietetic values. It is sweet in nature and is attributed to the spleen, lung, and kidney meridians. It has the effects of tonifying Qi and nourishing Yin, invigorating the spleen, moistening the lung, and benefiting the kidney. As a food material, it can be made into various functional foods with medicinal and health-care values, and has significant dietetic value. At present, the concept of health preservation and health care has been deeply rooted in society, and Polygonatum sibiricum products are increasingly accepted and loved by people. The most commonly used part of Polygonatum sibiricum for health food is the rhizome, while the stems and leaves also have beneficial effects such as anti-fatigue, anti-aging, and antioxidant, and are less researched and applied.

[0003] The production and consumption of sprout vegetables have a long history in China. The most commonly known and consumed sprout vegetables are soybean sprouts, mung bean sprouts, and pea sprouts. Based on the habit of eating these popular vegetables, people have developed more vegetables that can be used as sprouts, such as peanut sprouts, radish sprouts, pepper sprouts, Chinese toon sprouts, corn sprouts, and broccoli sprouts. These sprouts have the characteristics of using seeds as the main material for germination, easy availability of materials, short cultivation period of sprouts, large harvest, good taste, and certain health benefits.

[0004] However, in the prior art, the seed of Polygonatum sibiricum is not easy to germinate, is prone to moldy and rotten, and the cultivation period is long, which hinders the research and promotion of Polygonatum sibiricum. Chinese patent CN 107439189A discloses a method for rapidly cultivating high-quality seedlings from Polygonatum sibiricum rhizomes, which specifically discloses the steps of pretreatment of Polygonatum sibiricum rhizomes, treatment of Polygonatum odoratum waste rhizomes, activation treatment, bud induction treatment, heat shock treatment, root induction treatment, small seedling transplanting, and field management. The method reduces the water content in the Polygonatum sibiricum rhizomes by directly air-drying after excavation, slows down the rate of decay of Polygonatum sibiricum, and provides conditions for rapid germination in the later stage. However, the patent is intended to obtain Polygonatum sibiricum seedlings, and its main purpose is to prepare for subsequent field transplanting. The purpose is to pursue the germination rate and field transplanting survival rate, rather than to cultivate edible bud seedlings of Polygonatum sibiricum. Therefore, the disclosed patent method is not aimed at cultivating edible Polygonatum sibiricum bud seedlings, and even if the bud seedlings in the disclosed content are edible, the edibility of the Polygonatum sibiricum buds is very low. SUMMARY

[0005] In order to solve the above problems, a Polygonatum sibiricum bud seedling and a cultivation method thereof are provided. The Polygonatum sibiricum bud seedling is obtained by sequentially performing mother body pretreatment, bud induction culture, and bud seedling culture. The mother body is pretreated, so that through the bud induction culture and the bud seedling culture, on the one hand, the germination rate of Polygonatum sibiricum is improved, and on the other hand, the stem length and stem thickness of the Polygonatum sibiricum bud seedling are improved, and the edibility of the Polygonatum sibiricum bud seedling is improved. Through the cultivation method, the quality and edibility of the Polygonatum sibiricum bud seedling are effectively improved, the cultivation time of the Polygonatum sibiricum bud seedling is effectively shortened, and the promotion of the Polygonatum sibiricum bud seedling is facilitated.

[0006] The cultivation method of the Polygonatum sibiricum bud seedling provided in the present application mainly aims at the edibility as the main cultivation purpose, focuses on the overall edibility and edible value of different parts such as stems, tender buds, tender leaves, and young rhizomes, and directly and clearly aims at the edibility. There is no hard requirement for the growth conditions such as the propagation rate and survival rate of the remaining Polygonatum sibiricum rhizomes after harvesting the bud seedlings. Some material types can even not need subsequent propagation, and the harvesting of the bud seedlings is the end of the cultivation period.

[0007] According to one aspect of the present application, a cultivation method of a Polygonatum sibiricum bud seedling is provided, which comprises the following steps:

[0008] (1) mother body pretreatment; (2) bud induction culture; (3) bud seedling culture;

[0009] The mother body includes conventional rhizomes, sterile rhizomes, and Polygonatum sibiricum seeds;

[0010] The bud induction culture includes conventional water culture, double-layer water culture, and sand bed bud induction;

[0011] The bud seedling culture includes spraying of nutrient solution and nutrient solution water culture planting, and the culture temperature of the bud seedling culture is 4-25°C.

[0012] Optionally, the pretreatment method of the conventional rhizome includes sand storage and cold storage; the sand storage is that 1-3 year old healthy fresh rhizomes of Polygonatum are selected, mixed with fine sand with humidity of 60-70%, and buried in a leeward sunny place for wintering; the cold storage is that 1-3 year old healthy fresh rhizomes of Polygonatum are selected, cleaned, soaked in sodium hypochlorite solution for 15-20 min, rinsed with clean water, wrapped with water absorption paper, and then placed in a fresh-keeping bag for cold storage at 4-8℃ for 1-2 months.

[0013] Optionally, the pretreatment method of the sterile rhizome is that sterile rhizomes with a diameter of more than 2.5 cm and having not less than 3 obvious bud heads or bud bodies are selected, and subcultured at 0-8℃ for 1-2 times.

[0014] Optionally, the pretreatment method of the Polygonatum seed is that mature seeds are used in September-October, the seeds are cleaned, the outer black pulp is removed, the outer seed coat is reserved, and the seed coat is mixed with fine wet sand at a weight ratio of 1:1-3, and then packed in a mesh bag, and then the seed coat is layer-stored and sand-stored at 17-28℃, and then taken out in the next March-April; the humidity of the fine wet sand is 60-70%.

[0015] Optionally, the double-layer water culture includes the following steps: the pre-treated mother body is laid on the first cover, and the second cover is covered on the mother body, and the light-shielding cloth is covered on the second cover; and the nutrient solution is sprayed on the first cover and the second cover.

[0016] Optionally, the first cover and the second cover include sterile water moss or germination paper; the light-shielding cloth is of a breathable and light-shielding material; the spraying frequency of the nutrient solution is 3-6 times / day, and each time lasts for 3-5 min; and the nutrient solution is half to full MS macroelement solution.

[0017] Optionally, the conventional water culture includes the following steps: (1) the nutrient solution is filled in the water storage tank type planting box, the pre-treated mother body is wrapped with water moss or sponge, and the filter basket is placed in the water culture hole of the shelf;

[0018] (2) the thin film is covered on the mouth of the water storage tank type planting box, and the thin film is gradually uncovered after 7-10 days; and the thin film is completely removed after 15-20 days, and then the culture is transferred to the natural environment, and the temperature is 15-25℃, and the nutrient solution is replaced every 30 days;

[0019] The sand bed germination includes the following steps: the sand bed is filled with fine sand with a humidity of 60%, and the thickness of the fine sand is 20 cm; the pre-treated rhizome is uniformly and evenly laid in the sand bed in the same direction, and the plant spacing and row spacing are 5 cm; 5 cm of fine sand is covered, and the film is covered and kept moist, and the germination is carried out in the natural environment.

[0020] Optionally, the step (2) of bud induction culture further comprises spraying potassium sorbate solution to 70-80% of the rhizome sprouting every 2-3 days; the concentration of the potassium sorbate solution is 0.2 g / kg.

[0021] Optionally, the step of bud seedling management is performed after the aseptic rhizome sprouts, and the aseptic rhizome is cultured in the dark for 5-18 days, then the light is gradually increased, the light intensity is 1000-1500 lx, 8 hours per day, and the continuous light culture is performed for 2-7 days until the bud seedling stem and leaf are yellow-green and the stem height is 3-10 cm.

[0022] According to another aspect of the present application, the present application also provides a Polygonum sibiricum L. bud seedling obtained by the cultivation method.

[0023] The beneficial effects of the present application include but are not limited to:

[0024] 1. The cultivation method of the Polygonum sibiricum L. bud seedling according to the present application, which obtains the Polygonum sibiricum L. bud seedling by sequentially performing the mother body pretreatment, bud induction culture and bud seedling culture, and improves the bud emergence rate of the Polygonum sibiricum L. by pretreating the mother body, and improves the stem length and stem thickness of the Polygonum sibiricum L. bud seedling, and further improves the edibility of the Polygonum sibiricum L. bud seedling; the cultivation method effectively improves the quality and edibility of the Polygonum sibiricum L. bud seedling, effectively shortens the cultivation time of the Polygonum sibiricum L. bud seedling, and is beneficial to the popularization of the Polygonum sibiricum L. bud seedling; the cultivation method of the Polygonum sibiricum L. bud seedling provided by the present application mainly aims at the edibility, and focuses on the overall edibility and edible value of different parts such as stems, sprouts, leaves and young rhizomes, and the edibility purpose is direct and clear, and there is no hard requirement for the growth conditions such as the propagation rate and survival rate of the remaining Polygonum sibiricum L. rhizome after the bud seedling is harvested, and some material types can even not need subsequent propagation, and the bud seedling harvesting is completed, and the cultivation cycle is ended.

[0025] 2. The cultivation method of the Polygonum sibiricum L. bud seedling according to the present application, which further limits the pretreatment method of the conventional rhizome, the aseptic rhizome and the Polygonum sibiricum L. seed, and uses different pretreatment methods for different mother bodies, so as to reduce the mildewing and rotting phenomenon of the mother body, improve the bud emergence rate of the Polygonum sibiricum L., shorten the bud emergence time, and further shorten the cultivation cycle of the Polygonum sibiricum L. bud seedling.

[0026] 3. The method for cultivating the Polygonatum sibiricum sprouts according to the present application, by setting double-layer cultivation, the bottom and the upper part of the mother body are provided with the first cover and the second cover as the culture medium, to keep the mother body moist and store nutrients, by covering the shading cloth of breathable and light-proof material, to keep shading and moisture, by limiting the frequency, duration and nutrient solution of the sprayed nutrient solution, and taking the principle of "wet in dry", on the one hand, it can promote the germination of the mother body and improve the sprouting rate; on the other hand, the double-layer cultivation provided by the present application makes the stems have longer internodes and the stems are strong, which helps to grow the Polygonatum sibiricum sprouts with strong edibility.

[0027] 4. The method for cultivating the Polygonatum sibiricum sprouts according to the present application, by setting water moss or sponge to wrap the mother body, and placing it in a water filter basket for water culture, and further limiting the culture steps, culture time, culture temperature and other conditions of the conventional water culture, and limiting the time of covering the film and the time of removing the film, to facilitate the growth of the Polygonatum sibiricum sprouts with strong edibility.

[0028] 5. The method for cultivating the Polygonatum sibiricum sprouts according to the present application, when the conventional rhizome is used as the mother body, further comprising the step of spraying potassium sorbate solution, and limiting the concentration of the potassium sorbate solution, which is applied together with the nutrient solution, nutrient + bacteriostatic, effectively reducing the phenomenon of Polygonatum sibiricum rhizome rotting and improving the sprouting rate; when the sterile rhizome is used as the mother body, further comprising the step of sprout management, and specifically limiting the shading time, illumination time and illumination intensity, to obtain the Polygonatum sibiricum stem sprout with spiral leaves and improve its edibility. DETAILED DESCRIPTION

[0029] The present application will be described in detail below with reference to the examples, but the present application is not limited to these examples.

[0030] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. The reagents or raw materials used in the present application can be purchased through conventional channels, and unless otherwise specified, the reagents or raw materials used in the present application are used according to the conventional methods in the art or according to the product instructions. In addition, any method and material similar or equivalent to the described content can be applied to the method of the present application. The preferred implementation methods and materials described in the present patent are only for demonstration.

[0031] Example 1

[0032] (1) Mother body pretreatment: select sterile rhizomes with a diameter of 2.5 cm or more and with not less than 3 obvious bud heads or bud bodies, and subculture at 4℃ for 2 times;

[0033] (2) bud induction culture: the waterweed is soaked with water, and the water is squeezed out by hand. The wet waterweed is put into a suitable container, and the sterile waterweed is obtained after conventional autoclaving, and is cooled for standby. The pretreated rhizome is washed with sterile water to remove the culture medium attached to the surface of the rhizome, and is uniformly placed in a double-layer culture basket with a mesh. A layer of sterile waterweed is laid in the bottom of the double-layer culture basket in advance, and a layer of sterile waterweed is covered on the surface of the sterile rhizome. Finally, the sterile waterweed is covered with a light-shielding cloth made of breathable and light-proof material, and the culture temperature is 18℃;

[0034] (3) bud seedling culture: the full amount of MS nutrient solution is sprayed 4 times a day, each time for 5 min, and the culture temperature is 20℃;

[0035] (4) bud seedling management: after the sterile rhizome sprouts, the culture is shaded for 15 days, and then the light intensity is gradually increased to 1200lx for 8h / d. The continuous light culture is carried out until the harvest, and the rhizome sprouts of 10cm or with 2-5 nodes and spiral new leaves are obtained.

[0036] Example 2

[0037] (1) parent pretreatment: sterile rhizomes with a diameter of 2.5cm or more and with not less than 3 obvious bud heads or bud bodies are selected, and are subcultured at 0℃ for 2 times;

[0038] (2) bud induction culture: the pre-treated parent is wrapped with waterweed or sponge, and is placed in a filter basket. The filter basket is placed in the water culture hole of the shelf;

[0039] (3) the thin film is covered on the water tank type planting box, and is gradually removed after 7 days. After 15 days, the film is completely removed, and is cultured in a natural environment;

[0040] (4) bud seedling culture: the temperature is 15℃, the nutrient solution is replaced every 30d, and the height of the rhizome sprouts is about 10cm, with 2-5 nodes and spiral new leaves. Oxygen is supplied to the planting box by an oxygen generator every day to promote growth.

[0041] Example 3

[0042] (1) parent pretreatment: mature seeds are used in September-October, and the outer black pulp is removed. The outer seed coat is reserved, and is mixed with wet fine sand at a ratio of 1:1 and is packed into a mesh bag. The humidity of the wet fine sand is 70%, and the yellow sprout is obtained by layering sand at 25℃. The yellow sprout is washed with water and the excess fine sand is screened out.

[0043] (2) Bud induction culture: the rhizoma polygonati buds were evenly placed in the double-layer culture basket with mesh, a layer of germination paper was laid in the bottom of the double-layer culture basket in advance, and a layer of germination paper was covered on the surface of the rhizoma polygonati buds, and finally a light-shielding cloth made of breathable and light-proof material was covered on the germination paper, and the culture temperature was 18℃;

[0044] (3) Bud seedling culture: regularly spray nutrient solution, 6 times / day, the nutrient solution is full amount MS macroelement nutrient solution, after the bud seedling grows, remove the upper germination paper and light-shielding cloth, continue to culture, until the rhizoma polygonati bud seedling with one bud and one leaf is grown.

[0045] Example 4

[0046] (1) Mother pre-treatment: select 2-year-old healthy rhizoma polygonati fresh rhizome, wash, cut into segments with apical buds and 2-4 stem nodes, soak in sodium hypochlorite solution for 20 min, rinse with water, wrap with absorbent paper and put into a fresh-keeping bag at 8℃ for 1 month;

[0047] (2) Bud induction culture: soak the sphagnum moss in water, cut it short appropriately, squeeze out the water with hands, put the wet sphagnum moss into a suitable container, sterilize it by high pressure to obtain sterile sphagnum moss, and air dry for standby; after the pre-treated conventional rhizome is washed with sterile water again, it is evenly placed in a double-layer culture basket with mesh, a layer of sterile sphagnum moss is laid in the bottom of the double-layer culture basket in advance, and a layer of sterile sphagnum moss is covered on the surface of the sterile rhizome, and finally a light-shielding cloth made of breathable and light-proof material is covered on the sterile sphagnum moss, and the culture temperature is 25℃;

[0048] (3) Bud seedling culture: regularly spray nutrient solution, 3 times / day, the nutrient solution is full amount MS macroelement nutrient solution, spray sorbic acid potassium solution every 3 days, the concentration of sorbic acid potassium solution is 0.2 g / kg, after the rhizome germinates, stop spraying sorbic acid potassium solution, and obtain the pen-shaped rhizoma polygonati bud seedling with a stem height of about 10 cm and more than one internode.

[0049] Example 5

[0050] (1) Mother pre-treatment: select 1-3-year-old healthy rhizoma polygonati fresh rhizome, mix with fine sand with humidity of 60-70%, and bury in the leeward sunny place for wintering;

[0051] (2) Fill the sand bed with fine sand with humidity of 60%, the thickness of the fine sand is 20 cm, evenly place the pre-treated rhizome in the same direction on the sand bed, the plant spacing is 5 cm, cover with 5 cm fine sand, cover film and keep moist, and accelerate germination in natural environment;

[0052] (3) Periodically spray nutrient solution, 4 times / day, the nutrient solution is full amount MS macroelement nutrient solution, cultivate in the dark for 7 days after germination, increase light for 3 days after 7 days, light intensity is 1500 LX, culture temperature is 20°C, relative humidity of growth environment is 70%, light until the stem and leaf are yellow-green, get stem height of about 10 cm, with more than one internode of polygonatum tuberous seedling.

[0053] Comparative Example 1

[0054] Select the polygonatum seed collected in December every year, remove the pulp and wash with water, soak in 0.5 g / L GA 3 (gibberellin) for 30 min, take out the polygonatum seed and then soak in 8% carbendazim solution for 30 min. Take out the polygonatum seed and directly sow on the disinfected sand, then cover with 35 cm thick sand, spray 8% carbendazim solution 10-35 kg / 100 square meters. Put the long-rooted polygonatum seed into the germination treatment solution (water solution containing 85 mg / L 6 BA, 0.05 g / L GA 3, 40 g / L carbendazim) for 15-30 min, take out and put into sterilized sand, maintain relative humidity at 60-75%, temperature control at 20-28°C for 10-15 days. Take out the polygonatum seed after the germination treatment is completed, place it flat on the substrate, light intensity is 1000-2000 LX, light time is 6-10 h / day, temperature is 15-22°C, spray water 10-15 times to ensure the substrate is moist, culture for 15-20 days. Take out the polygonatum seed with just sprouted sprouts, remove the upper half of the sprouts with scissors, soak in the germination treatment solution again for 20-35 min, take out and put into sterilized sand, light for 6-10 h every day, maintain relative humidity at 80-95%, temperature at 15-22°C, culture for 15-20 days. Treat the multi-bud polygonatum seed with root growth solution for a total of three times, the interval time between the three treatments is 3-5 days, spray 10-35 kg / day per 100 kg of polygonatum seed, and spray in 3-5 times, culture temperature is 15-22°C, light for 10-13 h every day, light intensity is 1000-2000 LX, relative humidity is controlled at 60-75%, culture for 25-45 days. Take out the multi-bud polygonatum seedlings with a large number of root hairs, and transplant them into nutrient soil, culture temperature is 18-25°C, light for 8-12 h every day, light intensity is 700-1000 LX, relative humidity is controlled at 50-70%, get polygonatum seedlings.

[0055] Comparative Example 2

[0056] Fresh rhizome of Polygonatum sibiricum is collected in October to December every year, and is directly air-dried for 3 days after removing soil. The root hairs of the rhizome are removed, and the rhizome is washed with 6-10% carbendazim solution. The rhizome is cut into 2-4 stem segments, and is mixed with 10 kg of sterilized 3-mesh activated carbon. The relative humidity is maintained at about 50%, and the mixture is treated at 18°C for 15 days. The rhizome segments are placed in a low-temperature environment with a relative humidity of 65% at 6°C for 40 min. The treated rhizome segments are soaked in a water solution containing 70 mg / L 6-BA, 0.04 g / L GA3 and 30 g / L carbendazim for 30 min, and are then placed in sterilized sand. The relative humidity is maintained at 65%, the light intensity is 700 LX, and the light is on for 10 h per day at a temperature of 18°C. After 30 days, the rhizome segments grow sprouts. The rhizome segments with sprouts are subjected to heat shock treatment at 45°C for 5 s, and are then sprayed with water to keep the cloth wet. The relative humidity is maintained at 55%, and the temperature is 18°C. After 2 days, the rhizome segments are subjected to heat shock treatment at 45°C for 5 s again. After the heat shock treatment, the rhizome segments are treated with a water solution (rooting solution) containing 90 mg / L NAA and 15 g / L carbendazim for a total of three times. The interval between the three treatments is 3 days, and 20 kg of the rooting solution is sprayed per day in four times (i.e., 5 kg of the rooting solution is sprayed at a time). The temperature is 18°C, the light is on for 10 h per day, the light intensity is 1500 LX, and the relative humidity is controlled at 65%. After 40 days, the rhizome segments with a large number of root hairs are taken out, and are transplanted into nutrient soil (a mixture of 10 parts of garden soil, 5 parts of vegetable residue, 2 parts of river sand and 1 part of wood ash). The temperature is 20°C, the light is on for 12 h per day, the light intensity is 800 LX, and the relative humidity is controlled at 50%. The Polygonatum sibiricum seedlings are obtained.

[0057] Test Example

[0058] The growth of the Polygonatum sibiricum seedlings in Examples 1-5 and Comparative Examples 1-2 is investigated and counted. The average sprouting time, sprouting rate, number of new sprouts, single sprout growth, and reproduction coefficient are recorded. The results are shown in Table 1.

[0059] Table 1 Growth of Polygonatum sibiricum in different examples

[0060]

[0061] As shown in Table 1, the comprehensive performance of each example of the present application is better than that of Comparative Examples 1-2. The main differences are in the sprouting time, 20-day stem growth and rhizome subsequent reproduction.

[0062] From the average germination time, the aseptic rhizome requires the shortest time, which is significantly better than the seed and the conventional rhizome material. From the data of the examples, the longest germination time of the aseptic rhizome, the seed and the conventional rhizome is not more than 27 days, while the germination time of the seed in the two comparative examples is more than 45 days, which is significantly longer than the germination time of the examples.

[0063] From the stem growth amount at 20 days, the growth state of the seed in the examples and the seed in the comparative examples is similar, and the growth of the rhizome is quite different. The rhizome growth amount of the examples is significantly higher than that of the comparative examples. The rhizome growth in Example 5 is the largest, and the stem is the strongest.

[0064] From the rhizome propagation coefficient, the subsequent culture of the rhizome of the application after the bud seedling is obtained is obviously different from the comparative examples. The aseptic rhizome in Example 1 can continue to be regenerated and differentiated, and the propagation coefficient is 3.78. The seeds, aseptic rhizomes and conventional rhizomes in Examples 2-5 do not need to be cultured further after the bud seedlings are obtained and harvested. The culture of the bud seedlings is completed, which represents the end of the culture process. The rhizomes in Examples 2, 4 and 5 can be transplanted. The rhizomes in Comparative Examples 1-2 need to have a large number of root systems and more than one new bud that can germinate, and can be used for subsequent transplantation. After transplantation, it is necessary to survive as much as possible to obtain a high survival rate of the seedlings of the rhizome. This is obviously different from the purpose of the application, which only focuses on the culture of the bud seedlings.

[0065] In addition, although the test process to obtain the results in Table 1 cannot be directly shown in Table 1, it can be clearly seen from the above description of the examples and the comparative examples that the content of the application significantly simplifies the processing procedure and shortens the processing time compared with Comparative Examples 1-2.

[0066] The above is only an example of the application, and the protection scope of the application is not limited by these specific examples, but is determined by the claims of the application. The application can have various changes and variations for those skilled in the art. Any modification, equivalent replacement, improvement, etc. within the technical idea and principle of the application should be included in the protection scope of the application.

Claims

1. A method for cultivating Polygonatum sibiricum seedlings, characterized in that: The following steps are involved: (1) Pretreatment of mother plants; (2) Bud induction culture; (3) Bud culture; The mother body includes a conventional rhizome; The bud induction culture includes double-layer water culture and sand bed germination; The sprout culture includes spraying a nutrient solution, and the culture temperature of the sprout culture is 4-25° C.; the nutrient solution is a full-quantity MS macronutrient solution; The double-layer water culture comprises the following steps: laying the pretreated mother on a first cover, and covering the mother with a second cover, and the second cover is covered with a shade cloth; spraying a nutrient solution on the first cover and the second cover; the frequency of spraying the nutrient solution is 3 to 6 times per day, and each time lasts for 3 to 5 minutes; Conventional rhizomes in step (2) bud induction culture further includes spraying potassium sorbate solution once every 2 to 3 days until 70% to 80% of the rhizomes germinate; the concentration of the potassium sorbate solution is 0.2 g / kg.

2. The cultivation method according to claim 1, characterized in that Conventional rhizome pretreatment methods include sand storage and cold storage; the sand storage comprises selecting 1-3 year old healthy fresh rhizomes of Polygonatum sibiricum, mixing them with fine sand with a humidity of 60-70%, and burying them in a sheltered, sunny place to overwinter; the cold storage comprises selecting 1-3 year old healthy fresh rhizomes of Polygonatum sibiricum, washing them, soaking them in a sodium hypochlorite solution for 15-20 minutes, rinsing them with clean water, wrapping them with absorbent paper, and placing them in a fresh-keeping bag and refrigerating them at 4-8°C for 1-2 months.

3. The cultivation method according to claim 1, wherein The first covering and the second covering include sterile sphagnum moss; the blackout cloth is made of an air-permeable but light-proof material.

4. The cultivation method according to claim 3, characterized in that The sand bed germination includes the following steps: filling the sand bed with fine sand with a humidity of 60% and a thickness of 20 cm, placing the pretreated rhizomes evenly and flatly in the same direction on the sand bed with a plant spacing of 5 cm, covering with 5 cm of fine sand, covering with a film to retain moisture, and germinating in a natural environment.

5. The cultivation method according to claim 1, characterized in that When the conventional rhizome is used as the mother body, the method further includes a seedling management step, which includes shading culture for 5 to 18 days after germination, gradually increasing light intensity to 1000 to 1500 lx, 8 hours per day, and continuously culturing under light for 2 to 7 days until the stems and leaves of the seedlings turn yellow-green and the stem height is 3 to 10 cm.

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