Traditional Chinese medicine composition for improving sepsis-induced acute lung injury and preparation method thereof

Abstract

The present invention relates to the technical field of traditional Chinese medicine preparation, and specifically relates to a traditional Chinese medicine composition for improving sepsis-induced acute lung injury and a preparation method thereof. The traditional Chinese medicine composition is composed of the following raw materials: 15-25 parts of Astragalus membranaceus, 15-25 parts of Cornu Bubali, 20-30 parts of Acorus tatarinowii, 10-20 parts of stir-fried Lepidium apetalum, 10-20 parts of Polygonum cuspidatum, 10-20 parts of Immature Bitter Orange, 10-20 parts of Salvia miltiorrhiza, 10-15 parts of Ligusticum chuanxiong, 10-15 parts of Poria cocos, 10-15 parts of Houttuynia cordata, 5-15 parts of Platycodon grandiflorum, and 5-15 parts of Glycyrrhiza uralensis; the preparation method includes the following steps: Step 1: Make all the raw materials in the traditional Chinese medicine composition into coarse powder of 10-30 meshes, then add water with a weight 5-10 times that of the raw materials of the traditional Chinese medicine composition and decoct for 2-4 times, each time for 1-2 hours, and filter; Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.1-1.3, cool it, and sterilize it to obtain the traditional Chinese medicine composition.

Description

Technical field:

[0001] The invention belongs to the technical field of Chinese medicine preparation, and particularly relates to a Chinese medicine composition for improving sepsis-induced acute lung injury and a preparation method thereof. Background technology:

[0002] Sepsis is essentially a disordered host response to infection, leading to life-threatening multiple organ dysfunction. Acute lung injury is one of the most common complications in the early stage, which seriously threatens people's life, health and safety. Sepsis acute lung injury is characterized by diffuse alveolar damage, which is caused by excessive lung inflammation, apoptosis of alveolar epithelial cells, etc., causing damage to pulmonary capillary endothelial cells and alveolar epithelial cells, resulting in diffuse pulmonary interstitial and alveolar edema, leading to a variety of acute lung dysfunction. In recent years, the medical community has been seeking breakthroughs in treatment, but there is still a lack of specific treatment methods. How to reduce the mortality rate is still an important problem that needs to be solved urgently.

[0003] With the continuous development of Chinese medicine, the use of Chinese medicine to treat major diseases has aroused strong resonance in the world. The idea of ​​TCM to use the holistic concept as the starting point for disease treatment has been recognized by the medical community. According to TCM, the main pathogenesis of acute lung injury in sepsis is the weakness of the body's positive energy. If the body's qi is weak and the blood circulation is not smooth, the qi and blood are not transported, the internal organs are not nourished, and the toxic evil is generated internally, then the defense force is weak, the external toxins invade, and the internal and external toxins linger in the body, aggravating the loss of positive energy. Therefore, the current mainstream Chinese medicine prescriptions are mainly to treat acute lung injury in sepsis with the main purpose of invigorating qi, activating blood circulation and detoxifying. However, this type of prescription lacks the effect of clearing the bowels and clearing away heat, so the effect for emergency is not optimal. Summary of the invention:

[0004] In view of the deficiencies in the prior art, the present invention provides a Chinese medicine composition for improving sepsis-induced acute lung injury and a preparation method thereof.

[0005] Specifically, the present invention provides a traditional Chinese medicine composition for improving acute lung injury caused by sepsis, which is composed of the following raw materials: astragalus, buffalo horn, calamus, stir-fried scutellaria baicalensis seeds, polygonum cuspidatum, immature bitter orange, salvia miltiorrhiza, chuanxiong, poria and houttuynia cordata.

[0006] As a further illustration of the present invention, the Chinese medicine composition is composed of the following raw materials: 15-25 parts of astragalus, 15-25 parts of buffalo horn, 20-30 parts of calamus, 10-20 parts of stir-fried scutellaria baicalensis seeds, 10-20 parts of knotweed, 10-20 parts of immature bitter orange, 10-20 parts of salvia miltiorrhiza, 10-15 parts of chuanxiong, 10-15 parts of poria and 10-15 parts of houttuynia cordata.

[0007] The present invention also provides another traditional Chinese medicine composition for improving sepsis-induced acute lung injury. The traditional Chinese medicine composition is composed of the following raw medicinal materials: astragalus membranaceus, buffalo horn, acorus tatarinowii, stir-fried lepidium apetalum willd, polygonum cuspidatum, aurantii fructus immaturus, salvia miltiorrhiza, ligusticum wallichii, poria cocos, houttuynia cordata, platycodon grandiflorum, and licorice root.

[0008] As a further illustration of the present invention, the traditional Chinese medicine composition is composed of the following raw medicinal materials: 15 - 25 parts of astragalus membranaceus, 15 - 25 parts of buffalo horn, 20 - 30 parts of acorus tatarinowii, 10 - 20 parts of stir-fried lepidium apetalum willd, 10 - 20 parts of polygonum cuspidatum, 10 - 20 parts of aurantii fructus immaturus, 10 - 20 parts of salvia miltiorrhiza, 10 - 15 parts of ligusticum wallichii, 10 - 15 parts of poria cocos, 10 - 15 parts of houttuynia cordata, 5 - 15 parts of platycodon grandiflorum, and 5 - 15 parts of licorice root.

[0009] As a further illustration of the present invention, the traditional Chinese medicine composition is composed of the following raw medicinal materials: 20 parts of astragalus membranaceus, 20 parts of buffalo horn, 25 parts of acorus tatarinowii, 15 parts of stir-fried lepidium apetalum willd, 15 parts of polygonum cuspidatum, 15 parts of aurantii fructus immaturus, 15 parts of salvia miltiorrhiza, 12 parts of ligusticum wallichii, 10 parts of poria cocos, 12 parts of houttuynia cordata, 8 parts of platycodon grandiflorum, and 10 parts of licorice root.

[0010] The present invention also provides a preparation method for the above-mentioned traditional Chinese medicine composition for improving sepsis-induced acute lung injury, comprising the following steps:

[0011] Step 1: Make all the raw medicinal materials in the traditional Chinese medicine composition into coarse powder of 10 - 30 meshes, then add water with a weight 5 - 10 times that of the raw medicinal materials in the traditional Chinese medicine composition and decoct for 2 - 4 times, each time for 1 - 2 hours, and filter;

[0012] Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.1 - 1.3, then cool and sterilize to obtain the traditional Chinese medicine composition.

[0013] As a further illustration of the present invention, take the above-prepared traditional Chinese medicine composition and make it into clinically acceptable tablets, powders, capsules or granules through conventional preparation methods.

[0014] Scutellaria baicalensis: Bitter in taste and cold in nature. It belongs to the lung, gallbladder, spleen, large intestine, and small intestine meridians. It has the effects of clearing heat and drying dampness, purging fire and detoxifying, and stopping bleeding. It is mainly used to treat damp-warm disease, summer dampness, chest distress and nausea, damp-heat stagnation, diarrhea, jaundice, cough due to lung heat, high fever and restlessness, hematemesis due to blood heat, carbuncles and sores, etc.

[0015] Buffalo horn: Bitter and sour in taste and cold in nature. It has the effects of clearing heat and cooling blood, detoxifying and calming endogenous wind, and is used for symptoms such as coma and delirium in febrile diseases, macules, hematemesis, epistaxis, etc.

[0016] Acorus tatarinowii: Bitter in taste and warm in nature. It has the effects of promoting digestion by resolving dampness, opening orifices and resolving phlegm, and awakening the mind and improving intelligence, and is used for symptoms such as opening orifices and awakening the mind.

[0017] Fried Semen Lepidii: Pungent, bitter in taste, cold in nature. It has the effects of purging the lung to lower qi, resolving phlegm and relieving asthma, promoting diuresis to alleviate edema, and purging heat to expel pathogenic factors. It is used for lung abscess, edema, abdominal and thoracic hydrops, dysuria, chronic lung diseases, dyspnea and swelling due to heart failure, etc.

[0018] Polygonum cuspidatum: Slightly cold in nature, slightly bitter in taste. It belongs to the liver meridian, gallbladder meridian, and lung meridian. It dispels wind and dampness, disperses stasis and relieves pain, relieves cough and reduces phlegm, and is beneficial for promoting qi flow in the intestines and stomach.

[0019] Fructus Aurantii Immaturus: Bitter, pungent in taste, cold in nature. It is a qi-regulating traditional Chinese medicine and has the effect of breaking qi and reducing accumulation. It can be combined with Polygonum cuspidatum to achieve the purpose of purging the intestines and reducing excess.

[0020] Salvia miltiorrhiza: Slightly cold in nature, bitter in taste. It has the effects of removing stasis and relieving pain, promoting blood circulation and regulating menstruation, clearing the heart and relieving restlessness. It belongs to the category of drugs for activating blood circulation and regulating menstruation under the classification of drugs for removing blood stasis. It can be used for masses and accumulations, chest and abdomen stabbing pain, arthralgia due to heat pathogen, sores and ulcers, restlessness and insomnia; hepatosplenomegaly, angina pectoris, etc.

[0021] Codonopsis pilosula: Sweet in taste, neutral in nature. It has the effects of invigorating the middle-jiao and replenishing qi, quenching thirst, strengthening the spleen and nourishing the lungs, and nourishing blood and promoting fluid production. It is used for qi deficiency of the spleen and lungs, anorexia and lassitude, cough with deficiency asthma, qi and blood deficiency, sallow complexion, palpitation and shortness of breath, thirst due to fluid impairment, internal heat and consumptive thirst, etc.

[0022] Ligusticum chuanxiong: Warm in nature, pungent in taste. It has the effects of promoting blood circulation and qi movement, dispelling wind and relieving pain. It belongs to the category of drugs for activating blood circulation and relieving pain under the classification of drugs for removing blood stasis. It can be used for chest impediment pain, chest and hypochondrium stabbing pain, contusion and swelling pain, irregular menstruation, amenorrhea and dysmenorrhea, abdominal pain due to masses, headache, rheumatic arthralgia, etc.

[0023] Poria cocos: Sweet, light in taste, neutral in nature. It has the effects of promoting diuresis and percolating dampness, strengthening the spleen and regulating the stomach, and calming the mind. It is used for dysuria, edema and fullness, dizziness and palpitation due to phlegm-fluid retention, anorexia due to spleen deficiency, loose stools and diarrhea, restlessness of mind, palpitations and insomnia, cough and counterflow, vomiting and nausea, morning sickness, diarrhea, spermatorrhea, stranguria with turbid discharge, amnesia, etc.

[0024] Houttuynia cordata: Cold in nature and purging in function, it is good at clearing and resolving the heat of the lung. It is often combined with traditional Chinese medicines such as Scutellaria baicalensis, Fritillaria thunbergii, and Anemarrhena asphodeloides to treat diseases such as phlegm-heat and cough with asthma.

[0025] Explanation of the formula: Based on the method of supplementing qi, activating blood circulation and detoxifying in previous studies, combined with the characteristics of acute lung injury in sepsis, the present invention synergistically introduces the effects of clearing heat and opening orifices, and purging the intestines and reducing excess, so as to achieve a more targeted treatment method for patients with acute lung injury. In the present invention, Astragalus membranaceus, Cornu Bubali, and Acorus tatarinowii are used as the monarch drugs, clearing heat and opening orifices, supplementing qi and strengthening the healthy qi; fried Semen Lepidii, Polygonum cuspidatum, and Fructus Aurantii Immaturus are used as the minister drugs. Among them, the combination of fried Semen Lepidii and Polygonum cuspidatum has the effects of purging the lung and intestines, promoting blood circulation and removing blood stasis. When combined with Fructus Aurantii Immaturus, it can relieve fullness and excess; assisted by Salvia miltiorrhiza, Ligusticum chuanxiong, Poria cocos, and Houttuynia cordata; Polygonum cuspidatum, Salvia miltiorrhiza, and Ligusticum chuanxiong together play the role of promoting blood circulation, detoxifying and dredging the meridians, and Houttuynia cordata clears heat and detoxifies. Together with Platycodon grandiflorum guiding the drugs upward and Glycyrrhiza uralensis regulating the properties of the drugs, they jointly regulate the qi and blood and the functions of the zang-fu organs of the patients.

[0026] Compared with the prior art, the present invention has the following beneficial technical effects:

[0027] In the present invention, astragalus, water buffalo horn and calamus are all monarch drugs, which can clear away heat, invigorate the orifices, and invigorate qi and strengthen the body; fried scutellaria baicalensis seeds, polygonum cuspidatum and immature bitter orange are assistant drugs, wherein the fried scutellaria baicalensis seeds and polygonum cuspidatum are used together to have the effects of purging the lungs and clearing the bowels, promoting blood circulation and removing blood stasis, and when used together with immature bitter orange, the effects of relieving fullness and fullness can be relieved; salvia miltiorrhiza, chuanxiong, poria and houttuynia cordata are used as adjuvants; polygonum cuspidatum, salvia miltiorrhiza and chuanxiong have the effects of promoting blood circulation, detoxifying and dredging menstruation, and houttuynia cordata clears heat and detoxifies, and platycodon grandiflorum leads the medicine upward, and licorice harmonizes the medicinal properties, and together regulates the qi and blood of patients and the functions of internal organs. The Chinese medicine composition provided by the present invention can have a good improvement effect on patients with acute lung injury caused by sepsis. Description of the drawings:

[0028] Figure 1 The expression of HSPA12B in lung tissue of rats in each group given the Chinese medicine capsules obtained in the examples and comparative examples.

[0029] Figure 2 This is the ultrastructure of lung tissue in the sham operation group.

[0030] Figure 3 This is the ultrastructure of lung tissue in the CLP-induced sepsis acute lung injury model group.

[0031] Figure 4 This is the ultrastructure of lung tissue in the capsule intervention group of Example 2. Specific implementation method:

[0032] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0033] Example 1 A method for preparing a Chinese medicine composition:

[0034] The Chinese medicine composition is composed of the following raw materials: 15 parts of astragalus, 15 parts of buffalo horn, 20 parts of calamus, 10 parts of stir-fried scutellaria, 10 parts of polygonum cuspidatum, 10 parts of immature bitter orange, 10 parts of salvia miltiorrhiza, 10 parts of chuanxiong, 10 parts of tuckahoe and 10 parts of houttuynia cordata, 5 parts of platycodon and 5 parts of liquorice.

[0035] The preparation method of the above-mentioned Chinese medicine composition comprises the following steps:

[0036] Step 1: All the raw materials in the Chinese medicine composition are made into 10-mesh coarse powder, and then decocted twice with water 5 times the weight of the raw materials in the Chinese medicine composition, each time for 1 hour, and filtered;

[0037] Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.1, then cool and sterilize it to obtain the traditional Chinese medicine composition.

[0038] Example 2 A preparation method of a traditional Chinese medicine composition:

[0039] The traditional Chinese medicine composition is composed of the following raw materials: 20 parts of Astragalus membranaceus, 20 parts of Cornu Bubali, 25 parts of Acorus tatarinowii, 15 parts of stir-fried Lepidium apetalum, 15 parts of Polygonum cuspidatum, 15 parts of Fructus Aurantii Immaturus, 15 parts of Salvia miltiorrhiza, 12 parts of Ligusticum chuanxiong, 10 parts of Poria cocos, 12 parts of Houttuynia cordata, 8 parts of Platycodon grandiflorum, and 10 parts of Glycyrrhiza uralensis.

[0040] The preparation method of the above traditional Chinese medicine composition includes the following steps:

[0041] Step 1: Make all the raw materials in the traditional Chinese medicine composition into coarse powder of 20 meshes, then add water with a weight 7 times that of the raw materials of the traditional Chinese medicine composition and decoct for 3 times, 1.5 hours each time, and filter;

[0042] Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.2, then cool and sterilize it to obtain the traditional Chinese medicine composition.

[0043] Example 3 A preparation method of a traditional Chinese medicine composition:

[0044] The traditional Chinese medicine composition is composed of the following raw materials: 25 parts of Astragalus membranaceus, 25 parts of Cornu Bubali, 30 parts of Acorus tatarinowii, 20 parts of stir-fried Lepidium apetalum, 20 parts of Polygonum cuspidatum, 20 parts of Fructus Aurantii Immaturus, 20 parts of Salvia miltiorrhiza, 15 parts of Ligusticum chuanxiong, 15 parts of Poria cocos, 15 parts of Houttuynia cordata, 15 parts of Platycodon grandiflorum, and 15 parts of Glycyrrhiza uralensis.

[0045] The preparation method of the above traditional Chinese medicine composition includes the following steps:

[0046] Step 1: Make all the raw materials in the traditional Chinese medicine composition into coarse powder of 30 meshes, then add water with a weight 10 times that of the raw materials of the traditional Chinese medicine composition and decoct for 4 times, 2 hours each time, and filter;

[0047] Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.3, then cool and sterilize it to obtain the traditional Chinese medicine composition.

[0048] Comparative Example 1

[0049] Except that Cornu Bubali is replaced with Codonopsis pilosula and Acorus tatarinowii is replaced with Angelica sinensis, the other components, component dosages, preparation steps, etc. are the same as those in Example 2.

[0050] Comparative Example 2

[0051] Except that Polygonum cuspidatum is replaced with Codonopsis pilosula and Fructus Aurantii Immaturus is replaced with Angelica sinensis, the other components, component dosages, preparation steps, etc. are the same as those in Example 2.

[0052] Comparative Example 3

[0053] Except for subtracting two components of buffalo horn and acorus tatarinowii, the other components, component dosages, preparation steps, etc. are all the same as those in Example 2.

[0054] Comparative Example 4

[0055] Except for subtracting two components of polygonum cuspidatum and fructus aurantii immaturus, the other components, component dosages, preparation steps, etc. are all the same as those in Example 2.

[0056] ① Drug preparation

[0057] Take the above traditional Chinese medicine composition, and refer to the production method of traditional Chinese medicine capsules in Chinese Pharmacopoeia (2005 Edition) to prepare Huaxian capsules by the First Affiliated Hospital of Heilongjiang University of Chinese Medicine. The specification is 0.75 g / capsule, and the dosage is 0.75g - 1.5g / time for adults, 3 times a day. The optimal dosage of Huaxian capsules is 200mg / kg. Using this optimal dosage, dilute Huaxian capsules with 0.9% normal saline to 20mg / ml. In the Huaxian capsule intervention group of this experiment, intragastric administration was given at 10ml / kg.

[0058] ② Model establishment, grouping, administration, and sample collection

[0059] Sepsis acute lung injury model: 120 SPF wistar male rats (body weight: 200 ± 20g) were purchased from the Experimental Animal Center of Heilongjiang University of Chinese Medicine. After the rats were bought, they were first adaptively raised under SPF conditions, and the experiment was carried out 3 days later. The cecal ligation and puncture (CLP) method was used to establish a sepsis acute lung injury model. The specific operation was as follows: The rats were anesthetized with 1.0% sodium pentobarbital (20 mg / kg) by intraperitoneal injection, fixed in the supine position, the abdomen was disinfected and depilated, the skin was incised along the midline ventral white line, the abdominal cavity was opened, the cecum was found, the middle part between the end of the cecum and the ileocecal valve was ligated, two punctures were made at the ligated cecum with an 18-gauge needle, the cecum was gently squeezed to extrude part of the intestinal contents into the abdominal cavity, the cecum was placed back into the abdominal cavity, and the layers were sutured to close the abdominal cavity. After the operation, fluid resuscitation was carried out with normal saline (30mL / kg). After the sepsis rat model was successfully prepared, the related indexes of rat lung function such as tidal volume (VT) and peak expiratory flow (PET) were detected to ensure that the sepsis rat model caused lung function damage in rats. Observe the general state of the rats, and record the vital signs such as body temperature and respiration of the rats to establish a sepsis acute lung injury model.

[0060] Sham operation group model: 20 SPF Wistar male rats (body weight: 200±20 g) were purchased from the Experimental Animal Center of Heilongjiang University of Chinese Medicine. After the rats were bought, they were first adaptively raised under SPF conditions and the experiment was carried out 3 days later. The rats in the sham operation group were only laparotomized to expose the cecum without ligation and perforation. The general state of the rats was observed, and the vital signs such as body temperature and respiration of the rats were recorded to establish the sham operation group model.

[0061] Grouping and administration: 140 SPF Wistar rats with successful modeling as described above, male, body mass 200±20 g, were divided into 7 groups with 20 rats in each group, namely: sham operation group, CLP-induced sepsis acute lung injury model group, intervention group with the capsule of Example 2, intervention group with the capsule of Comparative Example 1, intervention group with the capsule of Comparative Example 2, intervention group with the capsule of Comparative Example 3, intervention group with the capsule of Comparative Example 4. Gastric gavage administration was started on the same day of modeling. The intervention group with the capsule of Example 2, the intervention group with the capsule of Comparative Example 1, the intervention group with the capsule of Comparative Example 2, the intervention group with the capsule of Comparative Example 3, and the intervention group with the capsule of Comparative Example 4 were respectively given the corresponding capsules by gastric gavage at a dose of 10 ml / kg once a day.

[0062] Sample collection: The experimental animals were sacrificed in batches at 6 h, 12 h, and 24 h respectively to obtain samples. After blood sampling at each time point after modeling, the rats were sacrificed by cervical dislocation, and the lung tissues were cut and placed in 4% formaldehyde solution for fixation, and then processed by dehydration, paraffin embedding, sectioning, dewaxing, etc., and stored in a refrigerator at 4°C for labeling and standby.

[0063] ③ Detection and analysis of animal experiment samples

[0064] In sepsis acute lung injury, how to reduce the degree of endothelial function injury and accelerate the repair rate of endothelial cells is a key issue related to the development trend of the disease. In public research, it has been confirmed that overexpression of HSPA12B can reduce the injury of vascular endothelium in lung tissue. Therefore, the present invention illustrates the improvement effect of the traditional Chinese medicine capsule on acute lung injury through the expression of HSPA12B.

[0065] Detection of HSPA12B by Western Blot: Pour 10% separating gel and 5% stacking gel. Add 1×SDS-PAGE protein loading buffer to the sample protein, add β-mercaptoethanol before loading, and denature at 100°C for 10 min. Load the sample after centrifuging at 10000 rpm for 5 min. The running voltage in the stacking gel is 8 V / cm (50 V). When reaching the interface of the separating gel, the voltage is adjusted to 415 V / cm (100 V), and continue electrophoresis for 40 - 60 min according to the protein Marker. Cut 4 PVDF membranes that completely match the size of the gel, rinse and soak them in methanol solution for 10 min, and then transfer them to the transfer buffer for equilibration for 15 min. Add a small amount of transfer buffer to a tray and soak 4 filter papers in it. Lay the filter papers and PVDF membranes. Assemble the transfer device in order, add transfer buffer. Transfer in an ice bath. Set it to 350 - 400 mA, 90 - 100 V, 1.5 - 2 h. After the electrotransfer is completed, disassemble the transfer device and mark by cutting a corner. Perform primary antibody and secondary antibody reactions. After the reaction, wash the membrane 3 times with PBST, 10 min each time. After blocking for 2 h, perform ECL color development, expose the film in a dark room and save the film. Finally, count the expression of HSPA12B in the lung tissues of rats in each group.

[0066] As Figure 1 shown, where CON is the blank control group, LPS is the CLP-induced sepsis acute lung injury model group, S2 is the capsule intervention group of Example 2, and D1 - D4 are the capsule intervention groups of Comparative Example 1 - Comparative Example 4 respectively; It can be seen from Figure 2 this that compared with the LPS model group, the expression of HSPA12B in the rats intervened with the traditional Chinese medicine capsules in Example 2 and Comparative Examples 1 - 4 of the present invention all increased significantly, and all showed a trend of increasing first and then decreasing. At the same time, compared with the traditional Chinese medicine capsules provided in Comparative Examples 1 - 4, the traditional Chinese medicine capsule provided in Example 2 of the present invention has a more significant effect on the expression level of HSPA12B, indicating that some of the monarch drugs and adjuvant drugs in the traditional Chinese medicine capsule provided by the present invention play an important role in improving sepsis acute lung injury and have a certain synergy.

[0067] SEM detection of pathological damage to the ultrastructure of lung tissue: Take a sample about 1 - 3 cm in size. After removing the cells and rinsing them in PBS, place them in a penicillin vial, add glutaraldehyde for fixation, wash twice with PBS, fix with osmium tetroxide, and wash twice with PBS. Dehydrate with acetone / isoamyl acetate (1:1) for 10 min, and then dehydrate with isoamyl acetate for 30 min. After treatment, soak in acetonitrile solutions of different concentrations for 15 - 20 min, and finally use 100% acetonitrile. After vacuum drying, take out the sample when its temperature rises to room temperature. Use the vacuum spraying method. Place the sample on the sample stage about 10 - 15 cm away from the evaporation source, rotate the sample, spray carbon first, and then spray gold. The spraying should be uniform. After completion, observe under the microscope. The ultrastructures of the lung tissues in the sham operation group, the CLP-induced sepsis acute lung injury model group, and the capsule intervention group of Example 2 are respectively as Figure 2 , Figure 3 and Figure 4 shown. It can be seen from the figure that the traditional Chinese medicine capsule provided by this application has an obvious improvement effect on the lung tissue, indicating that the traditional Chinese medicine capsule provided by the present invention can achieve the purpose of improving sepsis acute lung injury.

[0068] Typical clinical cases:

[0069] Case 1: Liu, female, 56 years old, on June 13, 2022. The patient was admitted to the ICU due to "persistent high fever, dyspnea, systemic edema, chest tightness, shortness of breath, and continuous rhonchi in both lungs". She had a history of hypertension. The diagnosis was sepsis acute lung injury and hypertension. Treat with anti-infection and symptomatic treatment. Administer 200 ml of the traditional Chinese medicine described in Example 2 by gavage once a day. On June 15, the rhonchi of the patient decreased, and there were no main symptoms such as abdominal pain, vomiting, and abdominal distension. On June 25, the patient's body temperature gradually dropped to normal, and the patient's vital signs were stable.

[0070] Case 2: Zhang, male, 48 years old, on October 26, 2022. The patient was hospitalized due to "persistent high fever, dyspnea, nasal flaring, chest tightness, shortness of breath, and continuous rhonchi in both lungs". He had no previous medical history. The diagnosis was sepsis acute lung injury. Treat with anti-infection and symptomatic treatment. Administer 200 ml of the traditional Chinese medicine described in Example 2 by gavage once a day. On October 28, the rhonchi of the patient decreased, and there were no main symptoms such as abdominal pain, vomiting, and abdominal distension. On November 3, the patient's body temperature gradually dropped to normal, and the patient's vital signs were stable.

[0071] It should be noted that in this article, terms such as "including", "comprising", or any other variant thereof are intended to cover non-exclusive inclusion, so that a process, method, article, or device including a series of elements not only includes those elements but also includes other elements not explicitly listed, or further includes elements inherent to such a process, method, article, or device.

[0072] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicine composition for improving acute lung injury caused by sepsis, characterized in that: The traditional Chinese medicine composition is composed of the following raw materials in parts by weight: 15-25 parts of astragalus, 15-25 parts of buffalo horn, 20-30 parts of calamus, 10-20 parts of stir-fried scutellaria, 10-20 parts of polygonum cuspidatum, 10-20 parts of immature bitter orange, 10-20 parts of salvia miltiorrhiza, 10-15 parts of chuanxiong, 10-15 parts of tuckahoe and 10-15 parts of houttuynia cordata, 5-15 parts of platycodon and 5-15 parts of liquorice.

2. The Chinese medicine composition for improving acute lung injury caused by sepsis according to claim 1, characterized in that: The Chinese medicine composition is composed of the following raw materials in parts by weight: 20 parts of astragalus, 20 parts of buffalo horn, 25 parts of calamus, 15 parts of stir-fried scutellaria, 15 parts of polygonum cuspidatum, 15 parts of immature bitter orange, 15 parts of salvia miltiorrhiza, 12 parts of chuanxiong, 10 parts of tuckahoe, 12 parts of houttuynia cordata, 8 parts of platycodon and 10 parts of liquorice.

3. A method for preparing a Chinese medicine preparation for improving acute lung injury caused by sepsis, characterized in that: The following steps are involved: Step 1: All the raw materials in the Chinese medicine composition of any one of claims 1-2 are made into 10-30 mesh coarse powder, and then 5-10 times the weight of the raw materials of the Chinese medicine composition are added with water to decoct for 2-4 times, each decoction is 1-2 hours, and filtered; Step 2: Concentrate the obtained filtrate into an extract with a relative density of 1.1-1.3, cool it, and sterilize it to obtain the Chinese medicine extract; The prepared Chinese medicine extract is prepared into clinically acceptable tablets, powders, capsules or granules through conventional preparation methods.

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