Lactobacillus rhamnosus JYLR-173 for assisting in improving malnutrition after dieting, bacterial agent and application thereof

Abstract

The present invention relates to the field of microbial application technology, and in particular to a Lactobacillus rhamnosus JYLR‑173 for assisting in improving malnutrition after dieting, and a bacterial agent and application thereof. Lactobacillus rhamnosus )JYLR‑173 was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on June 27, 2019, with the deposit number CGMCC No.18042, and the deposit address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing. Intragastric administration of Lactobacillus rhamnosus JYLR‑173 can significantly improve the malnutrition symptoms caused by dieting in rats, and can effectively regulate the intestinal microecological health of rats and repair intestinal damage. Therefore, Lactobacillus rhamnosus JYLR‑173 can help improve various problems caused by malnutrition after dieting, including dry hair, constipation, endocrine disorders, etc.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial application, and in particular to a Lactobacillus rhamnosus JYLR-173 for assisting in improving malnutrition after dieting, a bacterial agent and an application thereof. Background Art

[0002] In recent years, with the continuous improvement of material conditions, the proportion of overweight and obese people has increased rapidly. Dieting to reduce weight and control obesity is a relatively economical and accessible method, but long-term dieting may cause some harm to the body. Due to insufficient intake of nutrients in the body, lack of nutrients such as protein, sugar, fat, iron, calcium, etc., malnutrition may occur, which can be manifested as symptoms such as emaciation, dry hair, and a large amount of hair loss. Moreover, long-term dieting causes the gastrointestinal tract to not function normally, and the intestinal microecology is unbalanced, thereby affecting gastrointestinal motility and normal digestive function, and symptoms such as constipation, electrolyte disorders, and gastrointestinal inflammation. In addition, long-term dieting can induce endocrine disorders, functional decline of organs such as the pituitary gland and adrenal glands, and disordered hormone levels. The hypothalamus-pituitary-adrenal axis (HPA) is an important pathway for the central nervous system to regulate the gastrointestinal tract. The disorder of the secretion of pituitary adrenocorticotropic hormone (ACTH) will cause the secretion of glucocorticoids (GC) or cortisol (CORT) to be disordered, further aggravating gastrointestinal damage and anorexia, forming a vicious circle.

[0003] Malnutrition, gastrointestinal disorders and endocrine disorders caused by dieting can generally be alleviated by adjusting diet and supplementing the nutrients needed by the body. However, when gastrointestinal function is impaired, nutrients are not easily absorbed, and dietary adjustments alone are slow to take effect. If the symptoms are more serious, chemical drugs or traditional Chinese medicines need to be taken for treatment under the guidance of a doctor. Chemical drugs usually have certain side effects, and traditional Chinese medicines often have a poor taste and are less accepted by patients. Summary of the invention

[0004] In view of the technical problems in the prior art of alleviating malnutrition, gastrointestinal disorders and endocrine disorders caused by dieting, such as slow effect, large side effects or poor taste, the present invention provides a Lactobacillus rhamnosus JYLR-173 and a bacterial agent and application thereof for assisting in improving malnutrition after dieting.

[0005] In a first aspect, the present invention provides a method for improving malnutrition after dieting by using Lactobacillus rhamnosus JYLR-173. Lactobacillus rhamnosus ) JYLR-173 was deposited in the General Microbiology Center of China Microorganism Culture Collection on June 27, 2019, with the deposit number CGMCC No.18042, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0006] In a second aspect, the present invention provides a Lactobacillus rhamnosus JYLR-173 bacterial agent, comprising the above-mentioned Lactobacillus rhamnosus JYLR-173 bacterial cells, the number of which is 6×10 9 ~2×10 10 cfu / g.

[0007] Furthermore, the preparation method comprises the following steps:

[0008] (1) Prepare MRS liquid medium and MRS plate medium;

[0009] (2) Activating the preserved Lactobacillus rhamnosus JYLR-173 on an MRS plate medium, inoculating the activated strain into an MRS liquid medium at a mass percentage of 1%, and culturing to obtain a bacterial liquid;

[0010] (3) After centrifuging the bacterial solution, the bacterial cells were collected, washed with sterile physiological saline, and resuspended in reconstituted skim milk with a mass concentration of 15% to obtain a suspension; the concentration of the suspension was adjusted to obtain a bacterial suspension; the bacterial suspension was freeze-dried to obtain Lactobacillus rhamnosus JYLR-173 bacterial powder;

[0011] (4) The Lactobacillus rhamnosus JYLR-173 bacterial powder is mixed with maltodextrin to prepare a Lactobacillus rhamnosus JYLR-173 bacterial agent.

[0012] Furthermore, in step (2), the culture temperature is 37° C. and the culture time is 24 h.

[0013] Furthermore, in step (3), the concentration of the suspension is adjusted to 5×10 9 ~2.0×10 10 cfu / mL.

[0014] In a third aspect, the present invention also provides a use of the above-mentioned Lactobacillus rhamnosus JYLR-173 in the preparation of a product that assists in improving malnutrition after dieting. Preferably, the product is a medicine.

[0015] Furthermore, the product is a product for improving dry hair and malnutrition-induced hair loss caused by dieting.

[0016] Furthermore, the product is a product for promoting intestinal motility and improving constipation after dieting.

[0017] Furthermore, the product is a product for regulating ACTH, CRH and CORT hormone levels.

[0018] Furthermore, the product is a product that reduces intestinal damage and regulates intestinal flora.

[0019] The beneficial effects of the present invention are:

[0020] The present invention provides a Lactobacillus rhamnosus JYLR-173, which has been verified by rat experiments. Gavage of Lactobacillus rhamnosus JYLR-173 can significantly improve the malnutrition symptoms caused by dieting in rats, including improving dry hair and shedding, promoting defecation in rats, restoring the levels of ACTH, CRH and CORT, and effectively regulating the intestinal microecological health of rats and repairing intestinal damage. This shows that Lactobacillus rhamnosus JYLR-173 can assist in improving various problems caused by malnutrition after dieting, including dry hair, constipation, endocrine disorders, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0022] Figure 1 This is a diagram showing the analysis results of the diversity of intestinal flora in Example 6 of the present invention. DETAILED DESCRIPTION

[0023] In order to enable those skilled in the art to better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0024] Example 1 Isolation and identification of bacterial species

[0025] 1. Strain screening and purification

[0026] (1) Source of strain: Feces were collected from healthy people in Haiyang City, Yantai City, Shandong Province in July 2018, placed in sterile test tubes, and transported back to the laboratory through cold chain for standby use.

[0027] (2) Sample preparation:

[0028] ① Take 9 mL of sterilized saline (0.85%) and place it in a sterile conical flask, then add 1 g of feces obtained in step (1) and stir and shake at 4°C for 30 min to obtain a sample original solution, which is labeled 1#;

[0029] ② Dilute the solution in step ① to prepare samples with different concentration gradients, which are 10 -2 , 10 -3 , 10 -4 , 10 -5, 10 -6 , 10 -7 , numbered 2#, 3#, 4#, 5#, 6#, and 7#, for use.

[0030] (3) Prepare MRS plate culture medium containing 0.5% CaCO3:

[0031] 10g of peptone, 5g of beef powder, 5g of sodium acetate trihydrate, 2g of potassium hydrogen phosphate heptahydrate, 1mL of Tween 80, 0.05g of manganese sulfate tetrahydrate, 2g of triammonium citrate, 20g of glucose, 0.2g of magnesium sulfate heptahydrate, 5g of calcium carbonate, 15g of agar, and 1000mL of distilled water; after mixing the above raw materials, adjust the pH to 6.8, stir the bacterial solution, and sterilize it at 121°C and 0.1MPa for 20min, pour the sterilized culture medium into a plate, and let it cool for use.

[0032] (4) Cultivation of strains: Use a spreader to apply solutions 1#, 2#, 3#, 4#, 5#, 6#, and 7# onto MRS plate culture medium, and culture at 37°C under anaerobic conditions for 48 h;

[0033] (5) Select colonies according to the following colony characteristics:

[0034] The diameter is 1~2mm, the colonies are round, the edges are neat, slightly white with a bulge in the middle, and the calcium dissolution circle is large.

[0035] (6) Separation and purification

[0036] According to the colony characteristics of step (5), a single colony was picked and inoculated onto an MRS plate medium by streak method. The plate was cultured at 37°C under anaerobic conditions for 48 h. The operation was repeated 2 to 3 times. A single colony was picked and stored in a glycerol tube at -70°C.

[0037] 2. Identification

[0038] The strain JYLR-173 obtained after separation and purification was sent for identification by Sangon Biotech (Shanghai) Co., Ltd. During the identification process, the primers used were as follows:

[0039] Primer sequences:

[0040] 27F: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0041] 1492R: 5'-GGTTACCTTGTTACGACTT-3';

[0042] The gene sequence of strain JYLR-173 was obtained as follows:

[0043]

[0044] According to the identification results, strain JYLR-173 is Lactobacillus rhamnosus ( Lactobacillus rhamnosus ), whose taxonomic units are: Bacteria ; Firmicutes ; Bacilli ; Lactobacillales ; Lactobacillaceae ; Lactobacillu s, and was therefore named Lactobacillus rhamnosus JYLR-173.

[0045] Lactobacillus rhamnosus JYLR-173 was sent to China General Microbiology Center (CGMCC) for preservation, and the preservation information is as follows;

[0046] Strain name: Lactobacillus rhamnosus JYLR-173, classification name: Lactobacillus rhamnosus Lactobacillus rhamnosus , Deposit date: June 27, 2019, Deposit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Postal Code: 100101, Deposit number: CGMCC No.18042.

[0047] Example 2 Preparation of Lactobacillus rhamnosus JYLR-173 bacterial agent

[0048] (1) Preparation of MRS liquid medium

[0049] Weigh 10 g of peptone, 5 g of beef powder, 5 g of sodium acetate trihydrate, 2 g of potassium hydrogen phosphate heptahydrate, 1 mL of Tween 80, 0.05 g of manganese sulfate tetrahydrate, 2 g of triammonium citrate, 20 g of glucose, 0.2 g of magnesium sulfate heptahydrate, and 1000 mL of distilled water, mix the above raw materials, adjust the pH to 6.8, stir the bacterial solution, and sterilize at 121°C and 0.1 MPa for 20 min;

[0050] (2) Activate the preserved Lactobacillus rhamnosus JYLR-173 on MRS plate medium, inoculate the activated strain into MRS liquid medium at a mass percentage of 1%, and then culture at 37°C for 24 hours to obtain a bacterial solution;

[0051] (3) After centrifuging the bacterial solution, collect the cells, wash them with sterile saline, and resuspend them in reconstituted skim milk with a mass concentration of 15% to obtain a suspension; adjust the concentration of the suspension to 5×10 9 ~2×10 10 cfu / mL, and obtain a bacterial suspension; after freeze-drying the bacterial suspension, obtain Lactobacillus rhamnosus JYLR-173 bacterial powder;

[0052] (4) Lactobacillus rhamnosus JYLR-173 powder and maltodextrin (purchased from Baolingbao Biotechnology Co., Ltd.) were mixed in different proportions to prepare bacterial cells of 6×10 9 cfu / g, 1×10 10 cfu / g, 2×10 10 cfu / g of Lactobacillus rhamnosus JYLR-173 bacterial agent.

[0053] Example 3 Establishment of diet rat test model and experimental treatment

[0054] 1. Rat modeling

[0055] Sixty 6-week-old male SPF rats (purchased from Changsha Tianqin Biotechnology Co., Ltd., weighing 154g±6g) were selected and placed in an SPF laboratory with a 12-h light / 12-h dark cycle, an ambient temperature of 20-24°C, and a humidity of 40%-60%. The rat diet used for feeding contained 65% carbohydrates, 4% fat, 18% protein, 5% fiber, and 8% lime and minerals.

[0056] After 2 weeks of feeding, 12 rats were randomly selected as the control group, and the remaining 48 rats were used for modeling. The average daily food intake of rats in each group in the last 3 days before dieting (27g / d) was used as the standard daily food intake for subsequent experiments, and the rats in the control group maintained the standard daily food intake. The feeding amount of the modeling rats was reduced to 1 / 3 of the standard daily food intake, that is, 9g / d. Three weeks after the modeling operation, the modeling rats lost weight, had dry hair, and had a lot of hair loss. They had obvious symptoms of difficulty in defecation (defecation time was longer than 200 minutes and feces water content was less than 35%), and the modeling was initially judged to be successful.

[0057] 2. Experimental grouping

[0058] 48 rats with successful modeling were selected and randomly divided into 4 groups. Twelve rats without modeling were used as the control group, and a total of 5 groups were divided into experiments. The treatment of each group was as follows:

[0059] Control group: 12 normal rats, gavaged with 0.2g maltodextrin every day for four weeks.

[0060] Model group: 12 rats with successful modeling were given 0.2 g maltodextrin by gavage every day for four weeks.

[0061] Low-dose bacterial agent group: 12 rats with successful modeling, 0.2g of bacterial cells per day, the number of cells was 6×10 9 cfu / g of Lactobacillus rhamnosus JYLR-173 was administered intragastrically for four weeks.

[0062] Medium-dose bacterial agent group: 12 rats with successful modeling, 0.2 g of bacterial cells per day, the number of which was 1×1010 cfu / g of Lactobacillus rhamnosus JYLR-173 was administered intragastrically for four weeks.

[0063] High-dose bacterial agent group: 12 rats with successful modeling, 0.2g of bacterial cells per day, the number of cells was 2×10 10 cfu / g of Lactobacillus rhamnosus JYLR-173 was administered intragastrically for four weeks.

[0064] During the administration period, rats in each group were provided with a rat chow that met the standard daily food intake.

[0065] 3. Changes in rat weight

[0066] After the last administration, the body weight of each rat was measured at 9 am. The results are shown in Table 1 below.

[0067] Table 1 Body weight changes of rats (mean ± SD)

[0068]

[0069] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose bacterial agent group, P<0.05.

[0070] Compared with the control group, the rats in the model group lost weight. After oral administration of Lactobacillus rhamnosus JYLR-173, the weight of the rats in the low-dose bacterial agent group recovered slightly, and the weight of the rats in the medium-dose bacterial agent group basically recovered to the weight level of the rats in the control group. However, the weight recovery effect of the rats in the high-dose bacterial agent group was not as good as that of the rats in the medium-dose bacterial agent group, indicating that excessive oral administration of Lactobacillus rhamnosus JYLR-173 may affect the balance of intestinal flora and weaken the beneficial effects.

[0071] Example 4 Effect of Lactobacillus rhamnosus JYLR-173 on the hair condition of dieting rats

[0072] After the diet model was successfully established, the rats on a diet had insufficient nutrient intake, and their hair became dry and dull due to lack of adequate nutritional supply, and a large amount of hair loss occurred, thus simulating the malnutrition-type hair loss caused by dieting.

[0073] After four weeks of administration, the hair status of rats in each group was scored. The hair scoring method was as follows: dry, messy, falling hair, gray hair, 0 points; dry, messy, slightly falling hair, slightly gray hair 1 point; dry, messy, no falling hair, 2 points; dry, no messy, no falling hair, 3 points; shiny, smooth, no falling hair, 4 points. The results are shown in Table 2.

[0074] Table 2 Hair status scoring results

[0075]

[0076] According to the data in Table 1, the hair of normal rats in the control group was shiny, smooth, and did not fall off, while the hair of the dieting rats in the model group was dry, messy, and fell off, and the hair color was gray, indicating that the dieting rat model was successfully established. The hair condition of the dieting rats treated with Lactobacillus rhamnosus JYLR-173 bacterial agent was improved, and the hair was shiny, smooth, and did not fall off. This shows that Lactobacillus rhamnosus JYLR-173 can effectively improve the dryness and shedding of hair caused by dieting in rats.

[0077] Example 5 Effect of Lactobacillus rhamnosus JYLR-173 on constipation after dieting

[0078] 1. Changes in defecation time, number of stool particles, and water content of stool

[0079] After the last administration, rats in each group fasted overnight and had free access to water. After fasting for 12 hours, they were gavaged with 0.2 g of carbon powder solution. Six rats were randomly selected from each group, and each rat was fed in a single cage. The time of first black stool, feces water content, and number of feces particles within 3 hours of each group were statistically analyzed. The results are shown in Table 3.

[0080] Table 3 Defecation time, number of feces particles and feces water content of rats in each group (mean ± SD)

[0081]

[0082] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose bacterial agent group, P<0.05.

[0083] Long-term dieting causes malnutrition in rats, damages intestinal function, and leads to constipation. According to the data in Table 3, it can be seen that after oral administration of Lactobacillus rhamnosus JYLR-173, the first defecation time of rats in each bacterial agent group was significantly shortened compared with the model group. This result shows that Lactobacillus rhamnosus JYLR-173 bacterial agent can significantly shorten the defecation time.

[0084] Compared with the control group, the number of fecal particles in the model group decreased significantly in 3 hours. Compared with the model group, the number of fecal particles in the rats in each bacterial agent group increased significantly, indicating that the Lactobacillus rhamnosus JYLR-173 bacterial agent has a defecation-promoting effect on constipated rats.

[0085] Compared with the control group, the fecal water content of the model group was significantly reduced. Compared with the model group, the fecal water content of the rats in each bacterial agent group was significantly increased, indicating that intragastric administration of Lactobacillus rhamnosus JYLR-173 bacterial agent can effectively increase fecal water content.

[0086] 2. Small Intestinal Propulsion Rate

[0087] One hour after intragastric administration of the carbon powder solution, the remaining rats were killed and blood was collected from the vein for preservation. The abdominal cavity of the rats was cut open, and the stomach and intestine were completely taken out and placed on a photographic background board. The distance of carbon powder advancement and the total length of the small intestine ( ). The small intestinal propulsion rate was calculated according to the following formula:

[0088]

[0089] Table 4 Small intestinal propulsion rate of rats in each group (mean ± SD)

[0090]

[0091] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose bacterial agent group, P<0.05.

[0092] The results are shown in Table 4. It can be seen that the small intestinal propulsion rate of the model rats was significantly lower than that of the control group; while the small intestinal propulsion rate of the rats taking Lactobacillus rhamnosus JYLR-173 was significantly higher than that of the model group. This result shows that oral administration of Lactobacillus rhamnosus JYLR-173 to rats can effectively promote intestinal peristalsis and help defecation.

[0093] Example 6 Effects of Lactobacillus rhamnosus JYLR-173 on regulating intestinal flora and alleviating intestinal damage

[0094] 1. Changes in intestinal microbial diversity

[0095] One day before the end of the four-week feeding, about 10 g of fresh feces from the rats were collected and placed in a sterile container, which was quickly placed in a -80°C refrigerator for ultra-low temperature storage. The fecal microbial composition was detected by 16S rDNA sequencing. The Omega Mag-bindsoi1 DNA kit (purchased from Shanghai Suobao Biotechnology Co., Ltd.) was used to extract microbial genomic DNA samples from the feces of each group of rats according to the instructions and stored at -20°C for subsequent high-throughput sequencing analysis. The results are shown in the figure. Figure 1 shown.

[0096] from Figure 1 It can be seen that at the phylum level, Firmicutes ( Firmicutes ) and Bacteroidetes ( Bacteroidetes ) is the main dominant group, followed by Proteobacteria ( Proteobacteria ) and Actinobacteria ( Actinobacteria Compared with the control group, the intestinal phyla of Proteobacteria, Actinobacteria, and Soft-walled Bacteria in the model group rats were significantly higher than those in the control group. Tenericutes) was significantly reduced (P<0.05). Compared with the model group, the content of Proteobacteria, Actinobacteria, and Soft-walled bacteria in the intestines of rats in the low-dose bacterial agent group increased significantly, and the content of Bacteroidetes and Firmicutes decreased significantly, with a tendency to return to the structure of the flora in the control group. It can be seen that Lactobacillus rhamnosus JYLR-173 can increase the number of Proteobacteria, Actinobacteria, and Soft-walled bacteria in the intestines of model rats, improve the richness and diversity of the intestinal flora of rats, and regulate the intestinal microecological health of rats.

[0097] 2. Changes in the content of short-chain fatty acids in feces

[0098] About 50 mg of rat fecal samples were soaked in a saturated NaCl solution and then acidified with 10% sulfuric acid. The content of short-chain fatty acids (SCFAS) was determined using a gas chromatography-mass spectrometer (GC-MS). GC-MS conditions: chromatographic column, Rtx-Waxcolum, 30m×0.25mm×0.25μm; carrier gas was helium (gas flow rate 2mL / min, injection volume 2μL, split ratio 10:1); injection port temperature was 240℃; ionization temperature was 220℃; mass spectrometry scanning was full wave. The standard curve was drawn using the external standard method, and the concentration of SCFAS (μmol / g sample) was calculated based on the standard curve. The results are as follows.

[0099] Table 5 Effects of three short-chain fatty acids, acetic acid, propionic acid and butyric acid, in feces (mean ± SD)

[0100]

[0101] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose bacterial agent group, P<0.05.

[0102] As shown in Table 5, compared with the normal rats in the control group, the SCFAS content of the diet rats in the model group was significantly reduced. However, Lactobacillus rhamnosus JYLR-173 can significantly increase the SCFAS content. Compared with the model group, the acetic acid, propionic acid and butyric acid contents of the rats in the medium-dose bacterial agent group increased by 1.4 times, 2.3 times and 1.0 times, respectively. Therefore, it can be inferred that increasing the SCFAS content is one of the main ways for Lactobacillus rhamnosus JYLR-173 to repair intestinal function damage.

[0103] Example 7 Regulatory effect of Lactobacillus rhamnosus JYLR-173 on endocrine disorders after dieting

[0104] (1) Measurement of plasma hormone levels

[0105] After the plasma specimens were taken out of the -80°C freezer and thawed, the levels of CRH, CORT, and ACTH in the plasma were detected by enzyme-linked immunosorbent assay (ELISA). The operating procedures were in accordance with the instructions of the corresponding kits.

[0106] Table 6 Hormone levels in plasma of rats in each group

[0107]

[0108] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose bacterial agent group, P<0.05.

[0109] (2) Measurement of hormone content in each endocrine gland

[0110] One day before the end of four weeks of feeding, about 10 mL of fresh urine was collected from the rats and placed in a sterile container. After the rats in each group were killed, they were quickly decapitated and the brains were removed on ice in a clean bench. The hypothalamus and pituitary gland were separated, washed with ice saline, and stored in liquid nitrogen for inspection. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of CRH in the hypothalamus, ACTH in the pituitary gland, and CORT in urine. The operating procedures followed the instructions of the corresponding kits.

[0111] Table 7 Hormone contents in endocrine glands of rats in each group

[0112]

[0113] Note: a means compared with the control group, P<0.05; b means compared with the model group, P<0.05; c means compared with the low-dose group, P<0.05.

[0114] Combining Tables 6 and 7, it can be seen that compared with the control group rats, the ACTH concentration in the plasma and pituitary of the model group rats decreased, indicating that long-term malnutrition caused the pituitary function to decline and could not secrete enough hormones. Compared with the control group rats, the CRH and CORT concentrations in the plasma of the model group rats decreased (P<0.05), but the CRH content in the hypothalamus and the CORT content in the urine increased. Four weeks after intragastric administration of Lactobacillus rhamnosus JYLR-173, the ACTH, CRH, and CORT concentrations in the plasma and endocrine glands of the rats in the low-dose bacterial agent group recovered, while after intragastric administration of medium-dose Lactobacillus rhamnosus JYLR-173, the ACTH, CRH, and CORT concentrations in the plasma and endocrine glands of the rats basically returned to normal levels, and the hypothalamus and pituitary functions of the rats recovered, so that the hypothalamus-pituitary-adrenal axis was reactivated and the endocrine level returned to normal.

[0115] Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Without departing from the spirit and essence of the present invention, a person of ordinary skill in the art may make various equivalent modifications or substitutions to the embodiments of the present invention, and such modifications or substitutions shall be within the scope of the present invention. Any person of ordinary skill in the art may easily conceive of changes or substitutions within the technical scope disclosed by the present invention, and such changes or substitutions shall be within the scope of protection of the present invention.

Claims

1. A Lactobacillus rhamnosus JYLR-173 for assisting in improving malnutrition after dieting, characterized in that: Lactobacillus rhamnosus ( Lactobacillus rhamnosus ) JYLR-173 was deposited in the General Microbiology Center of China Microorganism Culture Collection on June 27, 2019, with the deposit number CGMCC No.18042, and the deposit address is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. A Lactobacillus rhamnosus JYLR-173 bacterial agent, characterized in that: The method comprises the bacterial cells of Lactobacillus rhamnosus JYLR-173 as claimed in claim 1, wherein the number of the bacterial cells is 6×10 9 ~2×10 10 cfu / g.

3. The Lactobacillus rhamnosus JYLR-173 bacterial agent according to claim 2, characterized in that The preparation method comprises the following steps: (1) Prepare MRS liquid medium and MRS plate medium; (2) Activating the preserved Lactobacillus rhamnosus JYLR-173 on an MRS plate medium, inoculating the activated strain into an MRS liquid medium at a mass percentage of 1%, and culturing to obtain a bacterial liquid; (3) After centrifuging the bacterial solution, the bacterial cells were collected, washed with sterile physiological saline, and resuspended in reconstituted skim milk with a mass concentration of 15% to obtain a suspension; the concentration of the suspension was adjusted to obtain a bacterial suspension; the bacterial suspension was freeze-dried to obtain Lactobacillus rhamnosus JYLR-173 bacterial powder; (4) The Lactobacillus rhamnosus JYLR-173 bacterial powder is mixed with maltodextrin to prepare a Lactobacillus rhamnosus JYLR-173 bacterial agent.

4. The Lactobacillus rhamnosus JYLR-173 bacterial agent according to claim 3, characterized in that In step (2), the culture temperature is 37°C and the culture time is 24 hours.

5. The Lactobacillus rhamnosus JYLR-173 bacterial agent according to claim 3, characterized in that In step (3), the concentration of the suspension was adjusted to 5×10 9 ~2.0×10 10 cfu / mL.

6. A use of Lactobacillus rhamnosus JYLR-173 as claimed in claim 1 in the preparation of a medicine for assisting in improving malnutrition after dieting, characterized in that: The drug is a drug that regulates the levels of ACTH, CRH and CORT hormones.

7. The use according to claim 6, characterized in that Medicines can improve dry hair and malnutrition-induced hair loss caused by dieting.

8. The use according to claim 6, characterized in that The medicine can promote intestinal motility and improve constipation after dieting.

9. The use according to claim 6, characterized in that The medicine can reduce intestinal damage and regulate intestinal flora.

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